Fission yeastMembraneMitogen-activaMutationsIntracellularEukaryoticPyrgMolecularCharacterizationESCRT-IIISecretoryHomologousInteractionRNAsAspergillusLysineLipidReceptorCytoplasmRibosomalBindEnzymesWhole genome duplicationSubunitNuclear exportLocalizationRegulateComplexesNucleusFilamentousZincEfficientlyTranscriptionDecayQuantitativeCellImportDiverseHighlyProcessDomains
Fission yeast4
- XPO1 (CRM1) originally was identified in the fission yeast Schizosaccharomyces pombe in a genetic screen, and investigators determined that it was involved in control of the chromosome structure. (wikipedia.org)
- Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus . (biorxiv.org)
- The fission yeast Schizosaccharomyces japonicus provides an attractive stripped-down model for metabolic compartmentalization in peroxisomes. (biorxiv.org)
- In the fission yeast S. pombe, the targeting complex, MTREC, consists of a core complex with the zinc-finger protein, Red1 ( Q9UTR8 ), and an RNA-helicase, Mtl1. (eu.org)
Membrane2
- Protein modules such as FVYE domains and PH domains that bind specifically to PtdIns 3-phosphate (PtdIns-3-P) and polyphosphoinositides, respectively, can direct such membrane targeting. (embl.de)
- Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)
Mitogen-activa1
- N-SLIT2 amplifies reactive oxygen species production in response to the bacteria by activating p38 mitogen-activated protein kinase that in turn phosphorylates NCF1, an essential subunit of the NADPH oxidase complex. (elifesciences.org)
Mutations1
- Juvenile neuronal ceroid lipofuscinosis (JNCL), one of the most frequent forms of the NCL storage diseases, is known to be caused by loss-of-function mutations in ceroid-lipofuscinosis neuronal protein 3 (CLN3), but its cell function has not been fully elucidated. (ac.ir)
Intracellular3
- The recruitment of specific cytosolic proteins to intracellular membranes through binding phosphorylated derivatives of phosphatidylinositol (PtdIns) controls such processes as endocytosis, regulated exocytosis, cytoskeletal organization, and cell signaling. (embl.de)
- Biosynthesis and intracellular targeting of the CLN3 protein defective in Batten disease. (ac.ir)
- Success will represent an unprecedented level of control over nanoscale motion, building an engineering capacity that will eventually be used to design protein nanoassemblies capable of sophisticated intracellular therapeutic functions such as genome repair. (nih.gov)
Eukaryotic2
- Exportin 1 (XPO1), also known as chromosomal region maintenance 1 (CRM1), is a eukaryotic protein that mediates the nuclear export of various proteins and RNAs. (wikipedia.org)
- Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, Nat. (db-engine.de)
Pyrg3
- molecular characterization of the niad and pyrg genes from penicillium camemberti, and their use as transformation markers. (liverpool.ac.uk)
- in this study, the nitrate reductase (niad) and orotidine-5'-monophosphate decarboxylase (pyrg) genes from penicillium camemberti were characterized, and their suitability as metabolic molecular markers for transformation was evaluated. (liverpool.ac.uk)
- pasop carries a selectable marker, pyrg, derived from aspergillus nidulans, and a strong promoter and a terminator of the amyb gene derived from a. oryzae. (liverpool.ac.uk)
Molecular2
- This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
- A next step in studying the complex interaction between host and microbe is to "directly" define the molecular factors that govern biological function (i.e. proteins, metabolites, posttranslational modifications) and delineate their associated mechanisms of action. (keystonesymposia.org)
Characterization2
- Despite the detailed in vitro characterization of the enzymatic properties of yeast Sac1p, the exact cellular function of this protein has remained obscure. (embl.de)
- functional characterization of nat/ncs2 proteins of aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. (liverpool.ac.uk)
ESCRT-III1
- Furthermore, the winged-helix domain of LEM2 activates the ESCRT-II/ESCRT-III hybrid protein CHMP7 to form co-oligomeric rings. (bvsalud.org)
Secretory4
- The PI3K/Akt/mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. (go.jp)
- For instance, how can cells control the diverse patterns of glycosylation of various secretory proteins in the endoplasmic reticulum and Golgi in an orderly manner with high accuracy? (go.jp)
- The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
- Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
Homologous2
- A homologous recombination system to generate epitope-tagged target genes in Chaetomium thermophilum. (db-engine.de)
- the filamentous fungus aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. (liverpool.ac.uk)
Interaction4
- The structural details of the interaction of XPO1 with its cargos were revealed two decades after the gene was identified. (wikipedia.org)
- Taking into account a crystal structure of Mtr4 interacting with a truncated, short Trf4 fragment (interaction of Mtr4 and short Air2 fragment is shown as well), one can argue for ring-interaction between the phenylalanine position and a tyrosine on the interacting protein (Mtr4). (eu.org)
- It is a large modular protein with several well-described interaction surfaces and partners. (eu.org)
- The interaction of Red1 with Ars2 ( O94326 ) is mediated by the Red1 EDGEI motif that recruits the Ars2-Cbc submodule, with cap-binding proteins, Cbc1 ( O14253 ) and Cbc2 ( Q9P383 ). (eu.org)
RNAs1
- The selection of RNAs is determined by binding of protein sub-modules to the MTREC core complex. (eu.org)
Aspergillus2
- comprehensive genomic analysis of cell wall genes in aspergillus nidulans. (liverpool.ac.uk)
- construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in aspergillus oryzae. (liverpool.ac.uk)
Lysine1
- Proteins synthesized at the ER are subject to covalent modifications that include N- and O-glycosylation, disulfide bond formation, and in some cases, proline and lysine hydroxylation. (stanford.edu)
Lipid1
- These regulators include canonical genes that control lipid metabolism as well as genes involved in ubiquitination, transcription, and mitochondrial function. (stanford.edu)
Receptor1
- It was later shown to be the nuclear transport receptor for cargos with leucine-rich nuclear export signals (NES). (wikipedia.org)
Cytoplasm1
- Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. (swisspalm.org)
Ribosomal1
- The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation. (db-engine.de)
Bind2
- We found that the FERM domain in the tail region, which is known to bind to lipids as well as other proteins, is essential for both nuclear and chloroplast positioning, whereas the proximal MyTH4 domain plays a supporting role in chloroplast transport. (go.jp)
- The C-terminal arm is a ZnF domain that can bind RNA and protein partners through adjacent surfaces. (eu.org)
Enzymes1
- Members of a subfamily of these enzymes share a specific domain that was first identified in the yeast Sac1 protein [1]. (embl.de)
Whole genome duplication1
- The duplication of the polymerase Trf and the zinc knuckle protein Air arose due to a whole genome duplication event in Saccharomyces cerevisiae ( 15004568 ). (eu.org)
Subunit1
- It forms a stable complex with CBC through its C-terminal disordered tail and interacts with RNA, proteins and multi-subunit complexes. (eu.org)
Nuclear export2
- For example, it is required for the nuclear export of HIV-1 RNA in complex with the viral protein Rev, an event that is a crucial part of the infection cycle. (wikipedia.org)
- Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
Localization1
- A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
Regulate1
- Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)
Complexes1
- Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes. (db-engine.de)
Nucleus1
- It exports several hundreds of different proteins from the nucleus. (wikipedia.org)
Filamentous1
- elimination of marker genes from transformed filamentous fungi by unselected transient transfection with a cre-expressing plasmid. (liverpool.ac.uk)
Zinc2
- In Fungi , it is formed by a RNA Helicase, Mtr4, in addition with a poly(A)polymerase, Trf4 or Trf5 and a RNA-binding zinc knuckle protein, Air1/2. (eu.org)
- The TRAMP complex consists of a RNA helicase (MTR4), a poly(A)polymerase (Trf4 or Trf5) and a RNA-binding zinc knuckle protein (Air1 or Air2). (eu.org)
Efficiently1
- Instead, damaged or incorrectly synthesized proteins must be rapidly and efficiently destroyed lest they form toxic aggregates. (stanford.edu)
Transcription1
- In humans the RNA-binding protein ARS2 ( Q9BXP5 ) has been implicated in transcriptional, post-transcriptional as well as early transcription termination activities. (eu.org)
Decay2
- Thus, GCGs contain proteins involved in translation initiation, translation control, and mRNA decay, which is consistent with their proposed role in the regulation ofs maternal mRNA expression. (rupress.org)
- Proteins of diverse protein families harbour such a motif often in two or three adjacent copies ( Foucher,2022 ), enabling mutually exclusive binding of different RNA decay/processing/splicing factors to the CBC-ARS2 complex ( Schulze,2018 ). (eu.org)
Quantitative1
- At its core, the laboratory develops and applies multiplexing quantitative proteomics tools to simultaneously track thousands of protein dynamics and associated post-translational modifications in an accurate and high throughput fashion. (keystonesymposia.org)
Cell2
- here, we present a comprehensive inventory of the cell wall-related genes in a. nidulans. (liverpool.ac.uk)
- The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
Import1
- As peroxisomes grow and mature, cargo import into the peroxisomal matrix takes place. (biorxiv.org)
Diverse1
- It has a positively charged patch on its surface that is bound by diverse protein partners involved in different steps of RNA processing through the acidic EDGEI motif. (eu.org)
Highly1
- In most proteins the motif is highly conserved among vertebrates, but in some cases also in for example, invertebrates and yeasts ( Foucher,2022 ). (eu.org)
Process1
- Some mRNAs are programmed for immediate translation, a process that remodels the protein coat and assembles a polysome. (rupress.org)
Domains1
- However, domains required for cargo transport in vivo have not been mapped. (go.jp)