Genes mice transgenic. Medical search. Frequent questions

Familial juvenile hyperuricemic nephropathy is caused by mutations in the UMOD gene encoding uromodulin. (elsevierpure.com)
Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Mutations of the B-cell CLL/lymphoma 6 ( BCL-6 or zinc finger protein 51) gene have been associated with LIP and mucosa-associated lymphoid tissue (MALT) lymphoma. (medscape.com)
This clonal expansion and subsequent growth of the tumors appears to be caused by mutations in oncogenes and tumor suppressor genes, and the whole process is accelerated by defects in genes required for maintaining genetic instability. (hopkinsmedicine.org)
Mutations in four or five such genes are required for a malignant tumor to form, while fewer mutations suffice for benign tumorigenesis. (hopkinsmedicine.org)
Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
[ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
Mutations in the RHO , GNAT1 , or PDE6B gene cause autosomal dominant congenital stationary night blindness. (medlineplus.gov)
Mutations in the RHO , GNAT1 , or PDE6B gene disrupt the normal signaling that occurs within rod cells. (medlineplus.gov)

The recent identification of mammalian circadian clock genes now makes it possible to examine time zone adjustments from the perspective of molecular events within the suprachiasmatic nucleus (SCN), the principal circadian oscillator. (jneurosci.org)
Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. (nature.com)
Mammalian parental imprinting is a form of epigenetic regulation that causes genes to be expressed from only one chromosome homolog according to parent-of-origin 1 , 2 . (nature.com)
To understand regulation of gene expression and to delineate the function of mammalian genes, we routinely create transgenic & gene knockout mice by performing DNA microinjection into mouse eggs, and genetic manipulation of mouse embryonic stem cells. (ccmb.res.in)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

Note that the primary transmission of France (3) isolate was performed on both hemizygous and homozygous mice. (cdc.gov)
Mice homozygous for a transgenic gene disruption exhibit decreased body size. (jax.org)
Homozygous mice die during embryonic development. (jax.org)
This causes the genes to become homozygous (same strain) at nearly all the loci on the chromosomes. (uwm.edu)
T2 mice were then crossed with the CaMKIIα-tTA activator line to create bi-transgenic mice (designated rT2/T2 when homozygous for the MAPT P301L transgene). (alzforum.org)

To investigate the possible effects of the expression of this tightly regulated gene in vivo, we produced transgenic mice in which CD40L expression was deregulated. (nki.nl)
CR-1 over-expression significantly increased in vivo xenograft tumor growth of HCC cells in nude mice and in vitro HCC cell proliferation, migration, and invasion. (aging-us.com)
To determine whether seizures affect GABA(A)-receptor gene transcription in vivo, a transgenic mouse line carrying a lacZ-fusion gene driven by GABA(A)-receptor δ-subunit promoter and upstream sequences was subjected to pentylenetetrazol (PTZ)-induced seizures. (psu.edu)
Using mouse models with distinct deletions in an intergenic region controlling imprinting across the Dlk1-Dio3 domain, we link changes in genetic and epigenetic states to allelic-expression and phenotypic outcome in vivo. (nature.com)
Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. (diabetesjournals.org)
Optical redox imaging of ex vivo hippocampal slices differentiates normal from Alzheimer's disease in mouse models World Molecular Imaging Congress, Miami Beach, FL 2022 Notes: Oral presentation. (upenn.edu)
Results: We examined R6/1 mice at 18-19 weeks of age using in vivo microdialysis. (lu.se)
2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
To model the effect in vivo, the authors used a transgenic mouse which over-expressed UMOD leading to salt-sensitive hypertension and interstitial nephritis . (medscape.com)

Behavioral Phenotyping of Transgenic and Knockout Mice (1st edition 2000, 2nd edition 2007, ISBN 978-0-471-47192-9), which was very well received. (wikipedia.org)
In an animal model of PFIC3, Abcb4 (Mdr2) knockout mice cannot excrete phospholipid into bile and develop progressive liver disease characterized by portal inflammation, proliferation of bile ducts, and fibrosis. (medscape.com)

A transgenic mouse model was developed by introducing a human mutant UMOD (C148W) cDNA under control of the mouse umod promoter. (elsevierpure.com)
To this end, transgenic mice expressing enhanced green fluorescent protein under a DCT-specific parvalbumin promoter were subjected to Mg(2+)-deficient or Mg(2+)-enriched diets. (nih.gov)
After implantation, a secondary DMR is established at the promoter of the Gtl2 gene, sustaining its repression from the paternal allele. (nature.com)
Induction of tyrosine hydroxylase in the locus coeruleus of transgenic mice in response to stress or nicotine treatment: lack of activation of tyrosine hydroxylase promoter activity. (rochester.edu)
To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin (Adipoq) gene promoters, as well as a tamoxifen-inducible Cre driven by the aP2 gene promoter (iaP2), were bred to the Rosa26R (R26R) reporter. (diabetesjournals.org)
rTg4510 mice ("r" for regulatable) are produced by crossing the 4510 responder line, carrying a human MAPT P301L cDNA downstream of a tetracycline operon-responsive element (TRE), to an activator line expressing a tetracycline-controlled transactivator (tTA) under control of the CaMKIIα promoter. (alzforum.org)
There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)

While imprinting perturbations are widely associated with developmental abnormalities, the intricate regional interplay between imprinted genes makes interpreting the contribution of gene dosage effects to phenotypes a challenging task. (nature.com)
Yet, the intricate form of epigenetic control over the parent-specific expression of multiple genes in an imprinted cluster poses difficulties when trying to decipher the relative contribution of changes in imprinted gene dosage to the resulting physiological phenotypes. (nature.com)
These findings indicate that disruption of Fgf14 contributes to the neuropathological and neurodegenerative phenotypes observed in rTg4510 mice. (alzforum.org)
Keeping in mind the caveat that not all phenotypes in rTg4510 mice are necessarily due to tau P301L overexpression, the following summarizes the extensive literature describing rTg4510 mice. (alzforum.org)
However, the roles of the DUB module that is comprised of UBP8, SGF11, SGF73, and SUS1 genes in those phenotypes are not yet understood. (bvsalud.org)

Expression of both the lacZ transgene and the endogenous MBP gene followed a common developmental pattern in mouse brain. (elsevierpure.com)
The ectopic expression of the transgene was associated with the development of DNase I hypersensitive sites at the site of insertion which was found to be within the intron 1 region of the endogenous MBP gene. (elsevierpure.com)
GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
To create T2 mice, a targeted insertion strategy was used to place the MAPT P301L transgene in a location in the mouse genome where no endogenous mouse genes would be disrupted. (alzforum.org)
In addition, shrinkage of the dentate gyrus occurs in the CaMKIIα-tTA activator line, although it is not yet known whether this is due to the expression of tTA or to the disruption of one or more endogenous mouse genes. (alzforum.org)
Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. (lu.se)
The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. (lu.se)
An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)

Crawley is at the origin of several widely employed tests used to evaluate, for example, anxiety-related and social behavior in rodents, especially mice. (wikipedia.org)
More recently, she developed the three-chamber social approach assay to evaluate mouse social behavior. (wikipedia.org)
She has been editor-in-chief of Neuropeptides, associate editor of Molecular Autism and Current Protocols in Neuroscience, and member of several editorial boards, among them Autism Research, Genes, Brain and Behavior, and Trends in Pharmacological Sciences. (wikipedia.org)
Genes, Brain and Behavior. (wikipedia.org)
The repression of specific genes that determine synaptic plasticity and neuronal development may account for suppressed electrophysiological activities of cortex and impaired behavior in Ttpa -/- mice. (elsevierpure.com)
Approximately 40% of transgenic mice satisfied the requirements of a stable experimental AVM model by replicating nidal anatomy, arteriovenous hemodynamics, and microhemorrhagic behavior. (thejns.org)
Implanting the glowing gene only forms the first step in the genetic study of cheating, and even if they locate the appropriate gene, figuring out how and why it regulates complex social behavior is a different matter entirely. (popsci.com)
The object of our study aimed to review and to correlate genes involved in ASD and those related to ultrasonic communication in animal model studies of language-based social behavior at the PubMed database. (bvsalud.org)

IgE)-deficient mice and a genetic background (C57BL/6)-matched strain. (cdc.gov)

Transgenic mice that were created that had a mutation in the gene which codes for the transcription factor (NGFIB). (freeonlineresearchpapers.com)
In PFIC3, a mutation in the gene ABCB4 on chromosome 7q21 encodes the protein MDR3, which functions in the translocation of phosphatidylcholine across the canalicular membrane. (medscape.com)

The results reported here strongly suggest that the 1.34-kb 5′-flanking region of the MBP gene contains cis-regulatory elements that confer developmental regulation of the MBP gene, although this region appears to lack elements that restrict its expression to the nervous system. (elsevierpure.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
This determined how hierarchical interactions between regulatory elements orchestrate robust parent-specific expression, with implications for non-imprinted gene regulation. (nature.com)
Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)
Posttranscriptional regulation of adrenal TH gene expression contributes to the maladaptive responses triggered by insulin-induced recurrent hypoglycemia. (rochester.edu)
In this study, we have further characterized deficits in the regulation of the nigrostriatal dopaminergic pathway in R6/1 transgenic HD mice. (lu.se)
Spt-Ada-Gcn5 acetyltransferase complex contains two different post-translational modifications, histone acetylation (HAT) module and deubiquitination (DUB) module, which are decisive in gene regulation and highly conserved in eukaryotes. (bvsalud.org)
To understand the role of the epigenetic co-repressor protein TRIM28 in the control of transposable elements (TEs) and how TEs control gene regulation in the developing and adult brain. (lu.se)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)

There are over 10,000 described species of reptile, and the genome of each species contains around 25,000 protein-coding genes. (mongabay.com)

These findings demonstrate that liver-specific CR-1 overexpression in transgenic mice deregulates signaling pathways and genes associated with HCC. (aging-us.com)
The activation of cell proliferation pathways in Ttpa -/- livers was indicated by the induction of genes that encode growth factor-binding proteins, mitogen-activated protein kinase kinase 3, and apoptosis inhibitor 6. (elsevierpure.com)
The Bert Vogelstein Laboratory seeks to develop new approaches to the prevention or treatment o ... f cancers through a better understanding of the genes and pathways underlying their pathogenesis. (hopkinsmedicine.org)
We used a mouse model of methylene diphenyl diisocyanate (MDI) asthma to identify biological pathways that may contribute to asthma pathogenesis. (cdc.gov)

Therefore, a mouse model was developed with a genetics-based approach that conditionally deleted the causative activin receptor-like kinase 1 ( Acvrl1 or Alk1 ) gene. (thejns.org)
The laboratory has advanced the understanding of gene therapy, angiogenesis, intracranial implantation of biodegradable polymers to treat malignant glioma, tumor genetics and proteomics, microchip drug delivery and drug resistance studies. (hopkinsmedicine.org)
The mouse genetics laboratory includes well-furnished infrastructure for culture of embryonic stem cells, cryopreservation of embryos, micro-manipulation of embryos, animal surgery, construction of transgenes & gene knock-out vectors, identification of transgenic & knock-out mice, and analysis of gene expression. (ccmb.res.in)

ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Intrastriatal infusion of both KCl and amphetamine rapidly increased the extracellular levels of DA in the dialysates in both R6/1 and wild-type mice, but the increase was significantly greater in the wild-type animals. (lu.se)
In contrast to wild-type mice, the transgenic UMOD mice had marked improvement in blood pressure with furosemide (an inhibitor of NKCC2). (medscape.com)

They're not a model system like a mouse or zebrafish, and they don't cure any diseases like naked mole rats . (popsci.com)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

Advances in the genetic manipulation of mice, modeling various aspects of the AD pathology, have provided an excellent tool to dissect the effects of genes, environment, and their interactions. (ox.ac.uk)
Unlike cancer arising in the human population, tumors in genetically engineered mouse models arise in mice with well defined genetic backgrounds where genetic variability can be minimized. (molvis.org)
Her translational research program focuses on testing hypotheses about the genetic causes of autism spectrum disorders and discovering treatments for the diagnostic symptoms of autism, using mouse models. (wikipedia.org)
On specific genetic backgrounds, some alleles cause partial or complete sex-reversal of chromosomally XY mice. (jax.org)
AVM pathogenesis can be understood as the combination of conditional Alk1 gene deletion during embryogenesis and angiogenesis that is hyperactive in developing and newborn mice, which translates to a congenital origin in most patients but an acquired condition in patients with a confluence of genetic and angiogenic events later in life. (thejns.org)
One naturally occurring genetic defect in mice results in an animal with no hair that is called a nude. (uwm.edu)
The researchers say they hope that exploring different gene functions in Anolis lizards will aid in the study of genetic defects in humans. (mongabay.com)
Mice that are widely used for genetic studies don't have a fovea and so studying in albinism in mice may not shed light on the link between albinism and impaired vision. (mongabay.com)
The ARVCF gene was isolated in the search for the genetic defect responsible for the autosomal dominant Velo-Cardio-Facial syndrome (VCFS), a relatively common human disorder with phenotypic features including cleft palate, conotruncal heart defects and facial dysmorphology. (nih.gov)
The laboratory uses both human brain samples and translational mouse models to study and treat genetic epilepsies such as Tuberous Sclerosis Complex and CDKL5 deficiency disorder, as well as more common forms of epilepsy, caused by status epilepticus, traumatic brain injury and neurodegenerative brain disorders. (upenn.edu)
Organisms which have altered or foreign genetic material, are now being referred to as transgenic. (freeonlineresearchpapers.com)
DNA microinjections is a common technique used for gene targeting as it involves manipulation and reintroduction of the genetic material into the same species. (freeonlineresearchpapers.com)
Despite their genetic distinctiveness, PFIC1 and PFIC2 have few clinical differences, and both are caused by the absence of a gene product required for canalicular export and bile formation. (medscape.com)
PFIC1 is caused by a genetic mutation in the ATP8B1 gene on chromosome 18q21-22. (medscape.com)

A 13-fold decrease in the expression of retinoic acid receptor-related orphan receptor-α mRNA predicts staggerer-like phenotype (ataxia and deficits of motor coordination) of Ttpa -/- mice. (elsevierpure.com)
Transgenic mice with AVFs had a recognizable phenotype of hereditary hemorrhagic telangiectasia but were less suitable for experimental modeling. (thejns.org)

These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)

Nevertheless, several proteins involved in DCT-mediated Mg(2+) reabsorption remain to be discovered, and a full expression profile of this complex nephron segment may facilitate the discovery of new Mg(2+)-related genes. (nih.gov)
This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
Cortex of Ttpa -/- mice revealed repression of genes encoding synaptic proteins, protein kinase C family members, and myelin proteins. (elsevierpure.com)
Mice have their own set of genes that code for the proteins that will make and sustain a mouse, much like we have our own genes that make us human. (mcgill.ca)
Latest achievements involve production of medically important proteins like anti-clotting factors through expression of the gene in lactating cows, goats and pigs. (freeonlineresearchpapers.com)
The proteins produced from these genes are necessary for normal vision, particularly in low-light conditions. (medlineplus.gov)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)

Gene-environment interaction research and transgenic mouse models of Alzheimer's disease. (ox.ac.uk)

Overexpression in mature T cells is compatible with life, but in one-third of the cases, mice developed atypical lymphoid proliferations which, occasionally, progressed into frank lymphomas. (nki.nl)
Sustained APPsα overexpression in aged mice with already preexisting pathology and amyloidosis restored synaptic plasticity and partially rescued spine density deficits. (springer.com)

To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. (jneurosci.org)
Enhanced expression of RNase L as a novel intracellular signal generated by NMDA receptors in mouse cortical neurons. (nih.gov)
We identified dopamine neurons that uniquely coexpress the Onecut3 and Nmur2 genes, and placed these in the periventricular nucleus with many synaptic afferents arising from neuromedin S + neurons of the suprachiasmatic nucleus. (nature.com)

Gene editing can also be used to curb invasive species populations. (mongabay.com)
Its most widely known application is to control mosquito populations, but with advances in gene editing this could be extended to invasive reptiles as well. (mongabay.com)
We demonstrate that the R6/2 transgenic mouse model expressing a short fragment of mutant HTT displays hypothalamic neuropathology with discrete loss of the neuronal populations expressing orexin, MCH, CART, and orexin at 12 weeks of age. (lu.se)
There was no effect of hypothalamic injections of QA on the neuronal populations expressing orexin, MCH, CART or oxytocin in neither HD mouse model. (lu.se)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
In this study we used a lentiviral vector that drives transgenic populations (BloÈmer et al. (lu.se)

The CaMKIIα-tTA transgene inserted on chromosome 12, resulting in a 508 kb deletion that affects five mouse genes: Vipr2 (vasoactive intestinal peptide receptor 2), Wdr60 (WD repeat-containing protein 60), Esyt2 (extended synaptotagmin-like protein 2), Ncapg2 (non-SMC condensin II complex, subunit G2), and Ptprn2 (protein tyrosine phosphatase, receptor type, N polypeptide 2). (alzforum.org)
The integration site of the MAPT P301L transgene is within the Fgf14 (fibroblast growth factor 14) gene on chromosome 14, resulting in a 244 Kb deletion that includes exon 1. (alzforum.org)
PFIC2 is caused by a mutation in the ABCB11 gene on chromosome 2q24 that encodes the bile salt export pump (BSEP). (medscape.com)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)

William Kaelin, Jr. was in 1995 engaged in the study of the von Hippel-Lindau tumor suppressor gene, and after isolation of the first full-length clone of the gene showed that it could suppress tumor growth in VHL mutant tumorigenic cell lines. (nobelprize.org)
In this work, we made use of the inducible SDHD-ESR mouse, a conditional mutant in the SdhD gene, which encodes the small subunit of MCII, and that acts as a tumor suppressor gene in humans. (ed.ac.uk)
[ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)

Phenotypic similarity of transmissible mink encephalopathy in cattle and L-type bovine spongiform encephalopathy in a mouse model. (cdc.gov)
Here we investigated the role of innate immunity in a mouse model of ALS. (nih.gov)
In humans with ALS, the analogous monocytes (CD14+CD16-) exhibited an ALS-specific microRNA inflammatory signature similar to that observed in the ALS mouse model, linking the animal model and the human disease. (nih.gov)
this model provides high sensitivity, cellular resolution, and ready quantification of levels of proenkephalin gene expression. (huji.ac.il)
Here we describe use of this transgenic model to study modulation of stress-regulated gene expression in the PVN by opiates. (huji.ac.il)
The nef gene product induces an LIP-like syndrome in a transgenic mouse model. (medscape.com)
The laboratory focuses on developing new therapies for brain and skull base tumors , and has established the first primary skull base chordoma xenograft mouse model. (hopkinsmedicine.org)
Here, we used a gene therapy approach to directly overexpress APPsα in the brain using AAV-mediated gene transfer and explored its potential to rescue structural, electrophysiological and behavioral deficits in APP/PS1∆E9 AD model mice. (springer.com)
The BACHD mouse model expressing full-length mutant HTT does not display any hypothalamic neuropathology at 2 months of age. (lu.se)
this mouse has become a popular model, as it phenocopies the tau pathology and pronounced neurodegeneration observed in human tauopathies, in addition to providing researchers with temporal control over mutant tau transgene expression. (alzforum.org)
Analysis of lung gene expression reveals a role for Cl- channels in diisocyanate induced airway eosinophilia in a mouse model of asthma pathology. (cdc.gov)
Expression changes in a core set of genes occurs independent of IgE in a mouse model of chemical-induced airway eosinophilia. (cdc.gov)

There are over 400 documented inbred strains of mice. (uwm.edu)
Two strains may be bred together to produce a mixture of the genes of each strain. (uwm.edu)
A modern mouse facility houses transgenic mice and other experimental mouse strains needed for creation of transgenic and gene knock-out mice. (ccmb.res.in)

rT2/T2 mice express even more tau P301L than rTg4510 mice, yet neurodegeneration is delayed and tau pathology occurs later and is less extreme in rT2/T2 mice than in rTg4510 mice. (alzforum.org)

This ®nding will lead to the development of lentiviral vectors with autoregulatory capacities that may be very useful for gene therapy to the brain. (lu.se)
Our technical expertise include lentiviral vectors, transgenic mice and stem cells cultures. (lu.se)

These mice have reduced glycogen stores in adipose tissue, liver, heart, and skeletal muscle, corresponding with decreased glycogen synthase activity and glycogen synthesis rate. (jci.org)

In contrast, mice kindled by repeated administration of initially subconvulsive doses of PTZ did not show a change in transgene expression, even shortly after the last PTZ-induced seizure. (psu.edu)
Bi-transgenic progeny constitutively express human tau P301L until transgene expression is inactivated by administration of the tetracycline analog doxycycline (dox). (alzforum.org)
The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. (lu.se)

The viral transactivating protein p40Tax activates the genes for interleukin-2 (IL-2) and its receptor's high-affinity alpha chain. (medscape.com)
The use of viral vectors to deliver foreign genes to the brain is highly logical states in the human brain (Eng et al. (lu.se)

succinate dehydrogenase, Sdh) genes cause familiar pheochromocytoma/paraganglioma tumors. (ed.ac.uk)
We are interested in how gene expression is regulated in the brain and how this process influence neurodegenerative diseases, psychiatric disorders and brain tumors. (lu.se)
[ 2 ] Targeted disruption of TP53 in the mouse leads to the development of various tumors (see image below). (medscape.com)
Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)
Tumors that developed in mice deficient in INK4A were enhanced by the topical application of carcinogens and ultraviolet light. (medscape.com)

Direct evidence linking the INK4A locus to tumorigenesis was provided by the targeted disruption of exon 2 of INK4A in mice. (medscape.com)

To confirm that the selected candidate genes were regulated by dietary Mg(2+) availability, the expression levels of solute carrier family 41, member 3 (Slc41a3), pterin-4 α-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1α (Pcbd1), TBC1 domain family, member 4 (Tbc1d4), and uromodulin (Umod) were determined by RT-PCR analysis. (nih.gov)
Moreover, they demonstrated that phosphorylated NKCC2 levels rose in tandem with UMOD gene dosage. (medscape.com)

Conventional MOs are typically used to constitutively disrupt targeted genes in an organism-wide manner. (cdc.gov)

The brain homogenates of three different tTA/TUL mice (no Dox) and three different tTA/TUL mice fed Dox chow for 7 days, along with non-transgenic brain homogenates and mice transgenic for only the TUL responder transgene were analyzed. (biomedcentral.com)
In both live and fixed brain preparations from these mice, detailed microanatomical features of EGFP-expressing interneurons were readily observed. (jneurosci.org)
We have explored genome-wide changes in mRNAs from brain cortex and liver of Ttpa-deficient (Ttpa -/- ) mice and wild-type (Ttpa +/+ ) mice. (elsevierpure.com)
Alk1 -floxed mice were crossed with deleter mice to generate offspring in which both copies of the Alk1 gene were deleted by Tagln -Cre to form brain AVMs in the mice. (thejns.org)
These molecular changes are evaluated for their potential as therapeutic targets and are often mutated genes, or genes that are over-expressed during the development of a brain cancer. (hopkinsmedicine.org)
Our laboratory uses large-scale genomic approaches to locate and analyze the genes that are mutated during brain cancer development. (hopkinsmedicine.org)
To understand the functional analyses of miRNAs and their role in neural stem cells by characterizing and identifiying known and novel RNAs bound to the mouse and human RNA induced Silencing Complex in various cell types of the brain. (lu.se)
To develop vector-based strategies of CRISPR/Cas9-mediated gene editing in the brain for experimental and therapeutic purposes. (lu.se)

We have generated four lines of transgenic mice carrying a transgene in which 1.34 kb of the 5′-flanking sequence of the mouse MBP gene was fused upstream of the coding region of the Escherichia coli lac Z gene in order to investigate developmental and tissue-specific expression of the MBP gene. (elsevierpure.com)
Instead of performing gene-editing on fertilized eggs or single-cell embryos, we performed gene-editing on unfertilized eggs while they are still physically attached to the ovary of adult female lizards," said Menke, a geneticist and developmental biologist. (mongabay.com)

The bi-transgenic mice accumulate an early burden of tau pathology in the form of argyrophilic tangle-like inclusions. (alzforum.org)

Regarding animal models in ASD, many studies focus on gene expression, cortical neuronal migration and cell maturation, and neural network deficits. (bvsalud.org)
Con respecto a los modelos animales en TEA, muchos estudios se centran en la expresión génica, la migración y maduración neuronal cortical y los déficits de la red neuronal, pero muy pocos son los estudios que relacionen directamente la comunicación oral animal y la expresión génica en áreas corticales del lenguaje. (bvsalud.org)

The remaining distal part of the IG-DMR was shown to bind pluripotency transcription factors in mouse embryonic stem cells (mESCs), exhibit active enhancer marks (H3K27ac), and nascent transcription 18 , 19 . (nature.com)
It involves introduction of modified DNA into embryonic stem-cells, which will take up the DNA and hopefully express the desired genes. (freeonlineresearchpapers.com)

However, histological signs of precancerous lesions, hepatocyte dysplasia or HCC formation were not observed in the livers of 3-, 6- or 8-month-old hepatocyte-specific CR-1-overexpressing transgenic mice. (aging-us.com)
Selective inductions of genes regulated by antioxidant response elements were detected in Ttpa -/- livers compared to Ttpa +/+ livers, suggesting increased oxidant stress in Ttpa -/- livers. (elsevierpure.com)

Heterozygous mice have skeletal abnormalities. (jax.org)

By elucidating the Mg(2+)-sensitive DCT transcriptome, new candidate genes in renal Mg(2+) handling have been identified. (nih.gov)

The scientists were able to reproduce these findings in other mice. (mcgill.ca)
Researchers are thus urged to be cautious in ascribing findings in these mice to the expression of transgenic tau P301L . (alzforum.org)
Some findings showed critical genes, which modulate several aspects of verbal communication. (bvsalud.org)

Rhodopsin then attaches (binds) to and activates the protein produced from the GNAT1 gene, alpha (α)-transducin. (medlineplus.gov)

If a transgene integrates in the middle of an important gene in the mouse's genome, it will disrupt this gene, much like a highway is suddenly rendered unusable thanks to a roadblock. (mcgill.ca)
The new molecular targets for cancer therapy are first located by large scale gene expression analysis, whole-genome scans for altered gene copy number and high throughput sequence analysis of cancer genomes. (hopkinsmedicine.org)
Knowing the base pair composition of the genome is only half the task done, the other half would be the identification of gene function. (freeonlineresearchpapers.com)
The genome with inactivated gene is introduced into embryo and then selected for. (freeonlineresearchpapers.com)
Lung tissue gene expression was assessed using whole-genome mRNA microarrays. (cdc.gov)

The first genetically modified animal was a transgenic mouse, created in 1974. (mongabay.com)
Mice are genetically altered to replicate conditions like cancer, Alzheimer's, diabetes, and other human diseases. (aerzte-gegen-tierversuche.de)

Indeed, all four genes show significant upregulation in the DCT of mice fed a Mg(2+)-deficient diet. (nih.gov)

This offers significant advantages for studying tumor pathogenesis and molecular mechanisms of oncogenesis caused by a single initiating oncogenic event introduced through the mouse germ line. (molvis.org)

Almkvist O, Basun H, Wagner SL, Rowe BA, Wahlund LO, Lannfelt L (1997) Cerebrospinal fluid levels of alpha-secretase-cleaved soluble amyloid precursor protein mirror cognition in a Swedish family with Alzheimer disease and a gene mutation. (springer.com)

Deletion of the TTP gene (Ttpa) in mice results in systemic deficiency of α-T and neurological dysfunctions described in patients with mutated Ttpa. (elsevierpure.com)
We generated mice that possess a heterozygous deletion of the PTG gene. (jci.org)
Therefore, we performed microarray analysis of adrenal medulla and kidney in order to identify other early gene expression changes elicited by SdhD deletion. (ed.ac.uk)

Current studies are aimed at the further characterization of the mechanisms through which these genes act, the identification of other genes that play a role in this tumor type, and the application of this knowledge to patient management. (hopkinsmedicine.org)

Our results revealed that each mutant tissue displayed different variations in their gene expression profiles affecting to different biological processes. (ed.ac.uk)

Procurement and maintenance of transgenic and knock-out mouse models of human diseases where such animals are available elsewhere without restriction and provide facility and expertise to use the same in drug screening. (ccmb.res.in)
Development of new transgenic and gene knock-out mice models of human diseases. (ccmb.res.in)

Stressful stimuli strongly induce proenkephalin gene expression within the paraventricular nucleus (PVN) of the hypothalamus. (huji.ac.il)
Figure 1: Cell-type diversity in the mouse hypothalamus. (nature.com)
In the present study, we wanted to examine whether transgenic HD mice display altered sensitivity to excitotoxicity in the hypothalamus. (lu.se)

We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)

These results show that transient changes in transcription of the GABA(A)-receptor δ-subunit gene occur after acute seizures, but not after kindling. (psu.edu)
Through the combined work of these three laureates it was thus demonstrated that the response by gene expression to changes in oxygen is directly coupled to oxygen levels in the animal cell, allowing immediate cellular responses to occur to oxygenation through the action of the HIF transcription factor. (nobelprize.org)

The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)

Moreover, new genes were identified that are potentially involved in renal Mg(2+) handling. (nih.gov)

Expression of mRNAs of pancreatic and L type RNase inhibitors as a function of age in different tissues of SAMP8 and BDF1 mice. (nih.gov)
In addition, the lacZ gene product was expressed in epithelial cells of certain nonneural tissues, namely kidney, epididymis, ureter, and seminal vesicles. (elsevierpure.com)
However, we found that the Cdkn1a gene was up-regulated in both tissues. (ed.ac.uk)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)

We also demonstrated upregulation of ETS-1 and ETS-2 target expressions in Tyrp-1-TAg mice when comparing with the same target expressions in control mice. (molvis.org)

This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)

The ARVCF gene encodes a protein containing two motifs, a coiled coil domain in the N-terminus and a 10 armadillo repeat sequence in the midregion. (nih.gov)

We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)

Then the DNA for some or all of the variable portion of the mouse antibody is merged with DNA for human immunoglobulin. (msdmanuals.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)

Uromodulin accumulation was observed in the thick ascending limb cells in the kidney of transgenic mice. (elsevierpure.com)

In this study, we investigated the role of embryonic gene Cripto-1 (CR-1) in hepatocellular carcinoma (HCC) using hepatocyte-specific CR-1-overexpressing transgenic mice. (aging-us.com)
These animals are used to study the function of the various genes. (uwm.edu)
They are used to study the diseases associated with defective genes. (uwm.edu)
These mice have deficiencies in their immune systems and thereby prove valuable in the study of immune diseases and cancer. (uwm.edu)
It can be used to study a human disease in mice, for example. (mcgill.ca)
In this study, we found that DUB module genes UBP8, SGF11, and SUS1, but not SGF73 positively regulate histone H2B DUB. (bvsalud.org)

In this paper we present several environmental factors implicated in the etiology of AD that have been tested in transgenic animal models of the disease. (ox.ac.uk)
Relevant mouse models of human uveal melanoma still remain to be developed. (molvis.org)
Current models of the clockwork posit interlocked transcriptional/post-translational feedback loops based on the light-sensitive Period ( Per ) genes and the Cryptochrome ( Cry ) genes, which are indirectly regulated by light. (jneurosci.org)
Aydin D, Weyer SW, Muller UC (2012) Functions of the APP gene family in the nervous system: insights from mouse models. (springer.com)
Breeding of currently available transgenic and knock-out mouse models. (ccmb.res.in)
1 In particular, morpholino oligonucleotides (MOs) have been used to block the expression of targeted genes in several invertebrate and vertebrate models. (cdc.gov)
Previous studies have shown that the nigrostriatal dopaminergic system is impaired in transgenic mouse models of HD. (lu.se)

Anatomy30

Organisms26

Diseases15

Chemicals and Drugs60

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Psychiatry and Psychology4

Phenomena and Processes51

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science3

Health Care1

Mutations9

Mammalian5

Homozygous5

Vivo9

Knockout mice2

Promoter8

Phenotypes5

Endogenous8

Behavior8

C57BL1

Mutation in the gene2

Regulation10

Protein-codin1

Pathways4

Genetics3

Wild-type mice3

Zebrafish2

Genetic14

Phenotype2

Metabolism1

Proteins7

Alzheimer's Disease1

Overexpression2

Neurons3

Populations6

Chromosome5

Tumor supp3

Model12

Strains3

Neurodegeneration1

Lentiviral2

Adipose tissue1

Transgene expression3

Viral2

Tumors5

Disruption1

Umod2

Constitutively1

Brain8

Developmental2

Pathology1

Neuronal2

Embryonic stem2

Livers2

Abnormalities1

Transcriptome1

Findings3

Activates1

Genome5

Genetically2

Deficient1

Pathogenesis1

19971

Deletion3

Characterization1

Mutant1

Human Diseases2

Hypothalamus3

Conditional1

Transcription2

Spontaneous1

Renal1

Tissues4

Upregulation1

Experimental1

Encodes a protein1

Mechanisms1

Immunoglobulin2

Kidney1

Study6

Models7