Genes jurkat cells. Medical search. Frequent questions

CD47-Dependent Regulation of Immune Checkpoint Gene Expression and MYCN mRNA Splicing in Murine CD8 and Jurkat T Cells. (nih.gov)
Secondly, we employed qRT-PCR and Western blotting to detect the mRNA and protein levels of GRHL2 in leukemia cell lines. (hindawi.com)
Then we investigated the demethylating effect of arsenic trioxide and 5-azacitidine on the mRNA and protein expression levels of GRHL2 in cell lines of acute leukemia. (hindawi.com)
The methylation level of the GRHL2 promoter region in acute leukemia patients and cell lines was significantly higher than the normal control group, and we found the elevated mRNA and protein levels of GRHL2 in acute leukemia cell lines after the use of the demethylation drug arsenic trioxide and 5-azacitidine. (hindawi.com)
Purification of total RNA including miRNA allows direct comparison of miRNA expression levels with those of housekeeping reference genes or any other mRNA of interest. (qiagen.com)
In these conditions, the conventional exon 1 was not identified in the mRNA from resting B cells. (biomedcentral.com)
Following substantial development, our screening platform uses freshly isolated and stimulated primary T cells which are first transduced with a lentiviral sgRNA library ahead of the introduction of Cas9 mRNA. (horizondiscovery.com)
DPP8 and DPP9 mRNA were upregulated in CCl₄ induced fibrotic livers but not in the lymphocytes isolated from such livers, while DPP9 was upregulated in EGF stimulated Huh7 cells. (eurekamag.com)

Inability of 50 Hz magnetic fields to regulate PKC- and Ca(2+)-dependent gene expression in Jurkat cells med. (emf-portal.org)
Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
Imbalances or disturbances in gene expression are sometimes deleterious for living things. (intechopen.com)
For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
In other words, expression of specific genes might have relevance to pathogenesis of diseases. (intechopen.com)
Subsequently array chambers were disassembled submerged in Agilent Gene Expression Buffer 1 and washed 1 minute in another dish with the same solution with a magnetic stirrer at 200 rpm at room temperature, followed by 1 minute in gilent Gene Expression Buffer 2 with a magnetic stirrer at 200 rpm at 37ºC and immediate withdrawal from the solution and air drying. (nih.gov)
Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. (nih.gov)
At this time, there is a paucity of information as to the expression of the Gimap genes in specific cell types. (hindawi.com)
We found a lower level of GRHL2 expression not only in acute leukemia patients but also in cell lines when compared with normal controls. (hindawi.com)
At the same time, arsenide trioxide and 5-azacitidine are associated with the inhibition of cellular proliferation of acute leukemia cells and also promote the elevated expression of TGF- β signaling pathway-linked proteins, including TGF- β , Smad2, Smad3, and Smad4. (hindawi.com)
Epigenetics indicates the alterations of gene expression levels mediated by nongene sequence changes. (hindawi.com)
Methylated DNA can prevent transcription factors from binding to it, resulting in low or no gene expression, which is an important alteration in the early initiation and development of malignant neoplastic diseases [ 7 ]. (hindawi.com)
research shows that about 90% of the blood system of malignant tumors with at least the height of single gene methylation is closely related since this is a reversible process of genetic modification can be through to handle to restore the normal expression of gene methylation, to achieve the purpose of prevention and treatment of tumor [ 10 ]. (hindawi.com)
Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
DBC1 functions as a coactivator for AR-V7 and is required for the expression of AR-V7 target genes including CDH2, a mesenchymal marker linked to CRPC progression. (cancerindex.org)
The RNA-stabilizing properties of RNAprotect Tissue Reagent prevent gene induction or down-regulation triggered by sample manipulation allowing you to preserve and analyze the gene expression profile. (qiagen.com)
Potential effects of forced CD20 expression on T-cell function were investigated by comparing CD20- and mock-transduced cytomegalovirus (CMV) specific T cells for cytolysis, cytokine release and proliferation. (haematologica.org)
Additionally, the relative expression of the P53, P21, and BCL-2 genes was evaluated using a real-time polymerase chain reaction. (celljournal.org)
Peptide-treated Jurkat cells were associated with increased expression of P21, and P53 genes. (celljournal.org)
DPP8 and DPP9 expression were measured in mouse splenic CD4⁺ T-cells, CD8⁺ T-cells and B-cells (B220⁺), human lymphoma cell lines and mouse splenocytes stimulated with pokeweed mitogen (PWM) or lipopolysaccharide (LPS), and in dithiothreitol (DTT) and mitomycin-C treated Raji cells. (eurekamag.com)
DPP8 and DPP9 expression were measured in epidermal growth factor (EGF) treated Huh7 hepatoma cells, in fibrotic liver samples from mice treated with carbon tetrachloride (CCl₄) and from multidrug resistance gene 2 (Mdr2/Abcb4) gene knockout (gko) mice with biliary fibrosis, and in human end stage primary biliary cirrhosis (PBC). (eurekamag.com)
DPP8 and DPP9 expression were upregulated in both PWM and LPS stimulated mouse splenocytes and in both Jurkat T- and Raji B-cell lines. (eurekamag.com)
We evaluated global gene expression patterns associated with IL32 expression. (biomedcentral.com)
We also evaluated the impact of proinflammatory molecules TNFα and IFNγ on IL32 expression and dedifferentiation in melanoma cell lines in vitro. (biomedcentral.com)
A significant proportion of established human melanoma cell lines express IL32, with its expression being highly correlated with a dedifferentiation genetic signature (high AXL/low MITF). (biomedcentral.com)
Cis -acting elements within this 5′ upstream region of the human IL32 gene appear to govern both induced and constitutive gene expression. (biomedcentral.com)
In the tumor microenvironment, IL32 expression is highly correlated with genes related to T cell infiltration, and also positively correlates with high AXL/low MITF dedifferentiated gene signature. (biomedcentral.com)
Nevertheless the over-expression from the traditional GR transcript didn't result in an increased degree of mGR (22), recommending how Rabbit Polyclonal to Histone H2A the membrane isoform isn't an unmodified GR localized for the cell surface area simply. (fabretp.org)
Alpha-lipoic acid upregulates antioxidant enzyme gene expression and enzymatic activity in diabetic rat kidneys through an O-GlcNAc-dependent mechanism. (ac.rs)
a Western blot analysis of CRBN protein expression in T-ALL cells. (biomedcentral.com)
c Knockdown of CRBN expression by sh-CRBN lentivirus at 5 days in 6 T-CEM, Molt4 and Jurkat cells. (biomedcentral.com)
Furthermore, this thesis outlined the impact of non-thermal plasma treatment on the gene expression level of immune cells. (uni-greifswald.de)
It became obvious that plasma exposure modulated the expression of several genes in both cell types. (uni-greifswald.de)
Differential expression of distinct target genes was further validated by quantitative PCR in the immune cell lines. (uni-greifswald.de)
Here, elevated gene expression levels of JUN and FOS in Jurkat cells and increased transcription of JUND in THP-1 cells in response to plasma treatment were made visible. (uni-greifswald.de)
JUN, FOS and JUND are components of the transcription factor AP-1, which is involved amongst others in gene expression of IL-8 and HMOX-1. (uni-greifswald.de)
While Jurkat cells showed no plasma-mediated regulation of cytokine expression, THP-1 cells revealed an increased IL-8 secretion after long plasma time duration (360 s). (uni-greifswald.de)
This indicates that GCLM transgene expression could be used for busulfan protection, hence selection of genetically modified cells. (seraphinstitute.org)

Finally, we studied the effects of arsenide trioxide and 5-azacitidine on the proliferation of leukemia cells and the TGF- β signaling pathway. (hindawi.com)
and promoted the proliferation and invasiveness of breast cancer cells both in vivo and in vitro. (cancerindex.org)
Additionally, DBC1 knockdown eliminated the up-regulation of MMP7, EMT-related proteins, and cell cycle-related proteins as well as the enhanced proliferation and invasiveness induced by ZNF326. (cancerindex.org)
ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. (reliatech.de)
Like BAFF, APRIL also stimulates the proliferation of B and T cells. (reliatech.de)
Analogous studies in a murine syngeneic tumor model using surrogate antibodies demonstrated significant synergy between LAG-3 and PD-1 blockade-combination treatment led to a marked improvement in therapeutic efficacy, increased T-cell proliferation, IFNγ production, and elicited durable immunologic memory upon tumor rechallenge. (aacrjournals.org)
When LPA was not present in the VZ, neural progenitor cells underwent proliferation, differentiation, and cell death. (molcells.org)
Laminin 521 has been shown to improve cell attachment, survival, and proliferation. (kactusbio.com)
Cell survival and proliferation were measured daily by Nucleocounter NC- 200 and flow cytometry using PI and 7-AAD staining protocols. (seraphinstitute.org)

simultaneously bind to major histocompatibility complex published a study of seven patients with severe streptococ- class II molecules and T-cell receptor molecules bearing a cal infections: SPE-A was detected in serum samples from four patients (27). (cdc.gov)
The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)
Classically, glucocorticoids (GCs)1 exert their immunomodulatory impact by activating the cytosolic glucocorticoid receptor (cGR), which translocates towards the nucleus and regulates gene manifestation (1). (fabretp.org)
The lifestyle of a glucocorticoid receptor (GR) in plasma membrane was initially reported inside a mouse lymphoma cell range (S-49) and it had been proposed to become functionally connected with glucocorticoid-induced cell loss of life (5). (fabretp.org)
The lifestyle of such a receptor was also reported inside a mouse pituitary cell range (22), suggesting a second gene can be mixed up in manifestation of the GC-binding proteins at least in the central anxious system. (fabretp.org)
The hypothesis can be backed by These data how the mGR result from the NR3C1 gene, as the cytosolic receptor (12). (fabretp.org)
To day liposome-based fluorescence amplification methods have been utilized (26), permitting the recognition of only 50 receptor substances per cell. (fabretp.org)
The channels interact with another type of calcium channel called ryanodine receptor 1 (RYR1) channels (produced from the RYR1 gene). (medlineplus.gov)
Genotype-phenotype correlations of DHP receptor alpha 1-subunit gene mutations causing hypokalemic periodic paralysis. (medlineplus.gov)

CD8 exists on the cell surface, where the CD8α chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8α. (peprotech.com)
Interleukin 32 (IL32) is produced by T cells, NK cells and monocytes/macrophages, but also by a subset of melanoma cells. (biomedcentral.com)
Through the use of this technique, Bartholome confirmed the current presence of the mGR on CCRF-CEM cells and proven how the mGR can be physiologically within monocytes and B-cells from healthful donors, while circulating T-lymphocytes had been consistently adverse (22). (fabretp.org)
In addition, alterations in the protein activation levels were analyzed in plasma-treated Jurkat, THP-1 cells and primary monocytes. (uni-greifswald.de)
In contrast to Jurkat and primary monocytes, the anti-apoptotic HSP27 was only induced in THP-1 cells in response to plasma exposure. (uni-greifswald.de)

Fucosyltransferase 4 is a protein that in humans is encoded by the FUT4 gene. (wikipedia.org)
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. (nih.gov)
The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. (nih.gov)
This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. (avivasysbio.com)
Positional cloning of lymphopenia ( lyp ) in the BB rat revealed a frameshift mutation in Gimap5 , a member of at least seven related GTPase Immune Associated Protein genes located on rat chromosome 4q24. (hindawi.com)
Gimap5 is a member of at least seven related GTPase Immune Associated Protein ( Gimap ) genes located within 150 Kilobases (Kb) on rat chromosome (RNO) 4 [ 2 , 3 ]. (hindawi.com)
Gimap5 was identified as the lyp gene in the BBDP rat through a frameshift mutation and premature truncation of the Gimap5 protein [ 2 , 6 ] and can be rescued in a P1-derived artificial chromosome (PAC) transgenic rat [ 7 ]. (hindawi.com)
Human CD20 has been proposed as non-immunogenic protein with a dual function as selection marker and suicide gene. (haematologica.org)
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (thermofisher.com)
Alternative splicing of this gene results in two transcript variants that encode the same protein. (thermofisher.com)
The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. (reliatech.de)
Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. (exbio.cz)
Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (peprotech.com)
Sequences that Confer Response to a Particular Stimulus Consensus Sequences CTNGAATNTT CTAGA Response to Heat Protein Factor Heat-shock transcription Gene Containing Sequences hsp70, hsp83, has a ring of glutamates near the inner entryway of the channel (Brelidze et al. (forextipsnow.com)
Autophagy-dependent and -independent involvement of AMP-activated protein kinase in 6-hydroxydopamine toxicity to SH-SY5Y neuroblastoma cells. (ac.rs)
KACTUS's Laminin 521 is a recombinant protein that can be used as a matrix protein for culture of human pluripotent stem cells and other cell types. (kactusbio.com)
Jurkat, CEM, and TF-1a cell lines were transduced with a lentiviral vector that expresses GCLM and green fluorescent protein (GFP) genes (Fig. 2A). (seraphinstitute.org)
Mutations in the CACNA1S gene change single protein building blocks (amino acids) used to make the CACNA1S protein, which alters the structure and function of calcium channels in skeletal muscle cells. (medlineplus.gov)

PCR arrays looking into target genes from the main signaling pathways indicated how the mGR will not exert its results through the transcriptional activity of the most common kinases in these leukemic cells, but RhoA signaling surfaced from our pathway evaluation. (fabretp.org)
Since some of the identified target genes are known to be associated with the MAPK pathways, the regulation of these cascades was further investigated by western blot analysis. (uni-greifswald.de)

Recent global analyses of gene transcripts revealed that specific transcription factors (TFs) and their networking systems physiologically correspond to the onset of human diseases, including cancer. (intechopen.com)
Molecular mechanisms of the initiation of transcription from TATA box have been well known as the most essential nuclear events in mammalian cells. (intechopen.com)
Alternative exon 1 structures may initiate transcription of CD5 using two different promoters, one being operative in the resting B cells and the other in any T cells and B cells only when activated. (biomedcentral.com)
As transcription silencing is key for establishing the HIV reservoir, significant efforts have been made to understand the mechanism that regulate HIV gene transcription, and the role of the elongation machinery in promoting this step. (biomedcentral.com)
However, while the role of the super elongation complex (SEC) in enhancing transcription activation of HIV is well established, the function of SEC in modulating viral latency is less defined and its cell partners are yet to be identified. (biomedcentral.com)
FUS inhibits the activation of HIV transcription by AFF4 and ELL2, and silences overall HIV gene transcription. (biomedcentral.com)
Concordantly, depletion of FUS elevates the occupancy of AFF4 and Cdk9 on the viral promoter and activates HIV gene transcription. (biomedcentral.com)
Finally, effects of FUS on HIV gene transcription are also exhibited genome wide, where FUS mainly occupies gene promoters at transcription starting sites, while its knockdown leads to an increase in AFF4 and Cdk9 occupancy on gene promoters of FUS affected genes. (biomedcentral.com)
Our observations show that FUS regulates both HIV and global gene transcription and modulates viral latency, thus can potentially serve as a target for future therapy that sets to reactivate HIV from its latent state. (biomedcentral.com)
Early studies on the regulation of gene transcription of the proviral human immunodeficiency virus (HIV) have laid the foundations to our current understanding of how metazoan transcription elongation is regulated [ 1 ]. (biomedcentral.com)

We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)

Our data substantiates the Gimap5 frameshift mutation as the primary defect with only limited contributions to lymphopenia from the remaining Gimap genes. (hindawi.com)
Positional cloning of the lyp gene revealed a frame shift mutation in Gimap5 (previously known as Ian5 or Ian4L1 ). (hindawi.com)
After multivariable adjustment, statistically significant associations between primary resistance, pre-transplantation measurable residual disease, DNMT3A mutation, or white blood cell count at diagnosis and early relapse were observed. (bvsalud.org)

Western Blot analysis of (A) RAW264.7 and (B) NIH-3T3 cell lysate using Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) (Product # MA1-91637). (thermofisher.com)

Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate. (thermofisher.com)
Lane 2: HeLa Cell Lysate (Prod. (enzolifesciences.com)

However, very little is known regarding the regulation of the gene activity in B cells, compared with T cells. (biomedcentral.com)
In order to study the transcriptional regulation of IL32 in these cell lines, we cloned up to 10.5 kb of the 5′ upstream region of the human IL32 gene into a luciferase reporter vector. (biomedcentral.com)
While short plasma treatment caused no alteration of the number of cells expressing IL-8 an up-regulation of the intracellular IL-6 level occurred after 30 s of plasma treatment. (uni-greifswald.de)

Relapse remains the leading cause of death in acute myeloid leukemia (AML) patients following allogeneic hematopoietic stem-cell transplantation (allo-HSCT), limiting the efficacy of allo-HSCT. (bvsalud.org)
MATERIALS AND METHODS: Patients with MDS-EB who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) from March 5, 2005, to November 8, 2020, were included. (bvsalud.org)
PLAIN LANGUAGE SUMMARY: Poor minimal residual disease clearance pretransplanation is an independent prognostic risk factor for long-term survival after allogeneic hematopoietic stem cell transplantation for patients with myelodysplastic syndrome with excess blasts. (bvsalud.org)
The use of Bcl-2 inhibitor Venetoclax (VEN) combined with hypomethylating agents or chemotherapy has shown efficacy in treating acute myeloid leukemia (AML) as frontline treatment and for relapse, allowing more patients to bridge to allogeneic hematopoietic stem cell transplantation (allo-HSCT). (bvsalud.org)
The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons. (exbio.cz)
CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. (exbio.cz)
Lack of sufficient engraftment in gene-modified hematopoietic stem/progenitor cells (HSPCs) therapies has been the biggest challenge. (seraphinstitute.org)

Therefore, we concluded that ZNF326 is highly expressed in breast cancer, is associated with poor prognosis, and plays a vital role in promoting the malignant phenotype of breast cancer cells by interacting with DBC1. (cancerindex.org)
1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
Some cationic anti-microbial peptides show a wide range of cytotoxic action versus malignant cells, which may lead to developing a novel group of antitumor medications. (celljournal.org)
Anal.Vol. Identification of p21 as a target of cycloheximide-mediated facilitation of CD95-mediated apoptosis in human malignant glioma cells. (forextipsnow.com)
CACNA1S gene mutations account for a very small percentage of all cases of malignant hyperthermia. (medlineplus.gov)

At least 11 mutations in the CACNA1S gene have been identified in people with hypokalemic periodic paralysis, a condition that causes episodes of extreme muscle weakness, usually in the arms and legs. (medlineplus.gov)
Lehmann-Horn F, Jurkat-Rott K, Rudel R. Periodic paralysis: understanding channelopathies. (medlineplus.gov)

Horizon scientists have recently successfully completed a pooled lentiviral CRISPR-Cas9 knockout screen in primary T cells. (horizondiscovery.com)
Gene knockout efficiency of our CRISPR Cas9 analyzed in nucleofected 293T, Jurkat, and T cells using TIDE analysis. (kactusbio.com)

Flow cytometry verified that the apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that of HDF and PBMCs. (celljournal.org)
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10 6 cells in a suspension. (exbio.cz)
Flow cytometry analysis (surface staining) of CD90 in Jurkat cells and SP2 cells with anti-CD90 (5E10) PE-Cy5. (exbio.cz)
Flow Cytometric Immunophenotyping Is Sensitive for the Early Diagnosis of De Novo Aggressive Natural Killer Cell Leukemia (ANKL): A Multicenter Retrospective Analysis. (bdbiosciences.com)
Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells. (bdbiosciences.com)
The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
This recent study used a FlowCam, Flow Imaging Microscope (FIM) assisted by machine learning software, to measure cell viability and concentration, and to quantify debris particulates. (fluidimaging.com)
First, cell survival and apoptosis induction was assessed in response to non-thermal plasma treatment by growth curves and flow cytometric assays. (uni-greifswald.de)
Moreover, modulation of cytokine production and secretion was examined in the different immune cell types and co-cultured THP-1 and HaCaT keratinocytes by ELISA or flow cytometry. (uni-greifswald.de)
The altered channels open more slowly than usual, reducing the flow of calcium ions into these cells. (medlineplus.gov)

Lane 3: Jurkat Cell Lysate (Prod. (enzolifesciences.com)

Imidazo[1,2-b]pyrazole-7-carboxamides induce apoptosis in human leukemia cells at nanomolar concentrations. (celljournal.org)
Riedl S, Leber R, Rinner B, Schaider H, Lohner K, Zweytick D. Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine. (celljournal.org)
Tested by its ability to induce IgM secretion by CpG/CD40L treated human naive B cells, at a concentration of 500 ng/ml. (reliatech.de)
Determined by its ability to induce plate adhesion of PHA-stimulated Jurkat cells. (peprotech.com)

Prof Hampton's research is focused on how understanding how cells sense and respond to oxidants, and how these pathways might be modulated to prevent and treat human disease. (otago.ac.nz)
1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
Human TRIM5α: Autophagy Connects Cell-Intrinsic HIV-1 Restriction and Innate Immune Sensor Functioning. (nih.gov)
Both GIMAP4 and GIMAP7 from human Jurkat cells [ 3 ] localize to the endoplasmic reticulum and Golgi apparatus while mouse Gimap3 from murine IL-3-dependent 32D myeloid precursor cells was expressed at the outer mitochondrial membrane [ 15 ]. (hindawi.com)
RNeasy Protect Mini Kits enable stabilization of RNA in tissue samples, RNA and DNA in sorted or cultured cells, RNA in human saliva samples and RNA in bacterial samples. (qiagen.com)
We investigated the use of human CD20 as suicide gene in T-lymphocytes. (haematologica.org)
Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)
In the present study, the anticancer activity of pleurocidin-like peptide WF3 isoform X2 (AMP-WF3), from the Poecilia Mexicana fish, against leukemic cell line Jurkat was evaluated, and the cytotoxicity compared with the effects on normal cells, including peripheral blood mononuclear cells (PBMCs) and human dermal fibroblast (HDF) cells. (celljournal.org)
The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
The human immunodeficiency virus (HIV) cell reservoir is currently a main obstacle towards complete eradication of the virus. (biomedcentral.com)
This technology will enable researchers to greatly accelerate their studies in primary T cells and opens the possibilities for building increasingly complex screening paradigms applicable to human disease. (horizondiscovery.com)
PeproTech's CHO cell-derived Recombinant Human sCD8α is a monomeric glycoprotein of 161 amino acid residues, which corresponds to the extracellular domain of CD8α. (peprotech.com)
Haynes BF, Denning SM, Singer KH, Kurtzberg J. Ontogeny of T-cell precursors: a model for the initial stages of human T-cell development. (bdbiosciences.com)
We analyzed RNA sequencing data from 53 in-house established human melanoma cell lines and 479 melanoma tumors from The Cancer Genome Atlas dataset. (biomedcentral.com)
However, the role of IL32 in human melanoma cells is less well understood. (biomedcentral.com)
The human melanoma cell lines were established from patient biopsies. (biomedcentral.com)
In CCRF-CEM cells, a human being T-cell lymphoblast-like cell range the recognition was possible just after enrichment of mGR+ cells using immunopanning strategies (19, 24, 25). (fabretp.org)
However, less is known about the plasma-mediated induction of intracellular signaling pathways in human immune cells, which play a leading part in the process of wound recovery and removal of pathogens. (uni-greifswald.de)
Therefore, this thesis examined the cellular effects of a non-thermal atmospheric pressure plasma treatment on human immune cells using the argon plasma jet kinpen 09. (uni-greifswald.de)

It is involved in cell differentiation, maintenance of cell differentiation state, tumorigenesis, and cell senescence, mainly through DNA methylation, histone acetylation, and RNA-related silencing [ 6 ]. (hindawi.com)
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (peprotech.com)
Differentiation of naïve CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-β1. (peprotech.com)
Leucocyte Typing VI: White Cell Differentiation Antigens. (bdbiosciences.com)

In HIV infected cells, knockout of FUS delays the gradual entry of HIV into latency, and similarly promotes viral activation in a T cell latency model that is treated with JQ1. (biomedcentral.com)

They can act as signalling molecules that regulate a number of life and death pathways in cells, and they are generated by the immune system to fight microbes. (otago.ac.nz)

Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)

Therefore, DNA microarray analysis was performed with the cell lines Jurkat and THP-1. (uni-greifswald.de)

The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
Administration of QIV with the two adjuvants, individually or combined, resulted in enhanced HA-specific serum ELISA IgG titers, serum hemagglutination inhibition (HAI) titers and splenic T cell responses as examined by IFN-γ and IL-4 enzyme-linked immunosorbent spot (ELISPOT) assays, 4-weeks post-prime and post-boost vaccination in BALB/c mice. (researchsquare.com)

The goal of this study was to explore a RIG-I agonist (SDI-nanogel) and a TLR7/8 agonist (Imidazoquinoline (IMDQ)‐PEG‐Chol) as adjuvants, when co-administered with a licensed quadrivalent inactivated influenza vaccine (QIV), and to determine the role of these adjuvants in directing helper T (Th) cell responses for their role in the immunoglobulin (Ig) class switching. (researchsquare.com)
Cereblon was a powerful helper for ARV-825 in T-ALL cells. (biomedcentral.com)
Here, the CD4+ T helper cell line Jurkat, the monocyte cell line THP-1 as well as the corresponding primary cells were investigated. (uni-greifswald.de)

The amount of mGR molecules per cell is low particularly. (fabretp.org)
In all investigated cell types the pro-proliferative signaling molecules ERK 1/2 and MEK 1/2 as well as the pro-apoptotic signaling proteins p38 MAPK and JNK 1/2 were activated in a plasma treatment time dependent manner. (uni-greifswald.de)

The positional cloning and subsequent identification of the Gimap5 gene on RNO4 were in part established through generation of the DR. lyp congenic rat line along with recombination events following our method of marker assisted breeding of DP with F344 rats [ 2 , 4 , 5 ]. (hindawi.com)
The CD5 T cell marker is present on a minute fraction of B cells. (biomedcentral.com)
Concurrent alteration of p16 and PTEN tumor suppressor genes could be considered as potential molecular marker for specific subgroups of NSCLC patients. (ac.rs)

Identification of Streptococcus pneumoniae genes associated with hypothiocyanous acid tolerance through genome-wide screening. (otago.ac.nz)

Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
B cells were isolated from tonsils, CLL blood and the control Daudi cell line cells, while T cells were obtained from normal peripheral blood (PB) and the control Jurkat cell line. (biomedcentral.com)
Activity of peripheral blood polymorphonuclear cells. (ac.rs)

miRNeasy Kits enable purification of total RNA from all types of animal tissues and cells, including difficult-to-lyse tissues and small tissue and cell samples. (qiagen.com)
The NEW miRNeasy Tissue/Cells Advanced Mini Kit - get excellent results faster without phenol/chloroform using our QIAzol free Advanced chemistry. (qiagen.com)
CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. (exbio.cz)
For example, tissue-forming cells (fibroblasts) migrate into the porous polymer framework to generate new skin indocator. (forextipsnow.com)

IgG2a also engages in high affinity interactions with Fc receptors on immune cells which can result in antibody-dependent cell-mediated cytotoxicity (ADCC) as well as antibody-dependent cellular phagocytosis (ADCP) by phagocytosing cells like macrophages 3-8 . (researchsquare.com)

Do not freeze the reagent or expose it to direct light during storage or incubation with cells. (bdbiosciences.com)

It was noted that light obscuration, one of the standard pharmacopeial methods for quantification of subvisible particle impurities, is not capable of discriminating between cells and other particulates (due to size, shape and opacity). (fluidimaging.com)
High-throughput microscopy methods that can provide morphological data are promising tools to evaluate particulates in cell-based produ cts. (fluidimaging.com)

Furthermore, after a 48-hour stimulation with PMA, the conventional exon 1 was used in activated B- as in the T-cells. (biomedcentral.com)

Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
In our validation screen, we performed a drug-gene interaction analysis by exposing an edited pool of primary T cells to prolonged treatment with a sub-lethal dose of a specific small molecule inhibitor. (horizondiscovery.com)
We took multiple time points and used T cells isolated from multiple donors, allowing for the analysis of response kinetics and evaluation of donor-specific effects. (horizondiscovery.com)
To validate our platform, we monitored the rate of response of the T cells to a control set of sgRNAs and compared the performance of the primary T cell screen directly and side-by-side to a Jurkat cell line analysis. (horizondiscovery.com)
We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)

Microdroplets can barcode more cells but worries persist about the differential chemistry and resulting biases accomplished in each droplet. (nature.com)

Using methyl thiazole tetrazolium salt reduction (MTT test), the effects of the AMP-WF3 on cell viability and toxicity were evaluated. (celljournal.org)
Busulfan is an agent that has higher toxicity on bone marrow stem cells and limited toxicity to other organs. (seraphinstitute.org)

Next, we used methylation-specific polymerase chain reaction (MSP) technology for detecting the methylation of GRHL2 in clinical samples with acute leukemia and cell lines. (hindawi.com)
Non IL32-expressing differentiated melanoma cell lines exposed to TNFα or IFNγ can be induced to express the three predominant isoforms (α, β, γ) of IL32. (biomedcentral.com)
Melanoma cell lines were treated with 1000 IU/mL TNFα and 100 IU/mL IFNγ (PeproTech US, Rocky Hill, NJ) for the indicated time points. (biomedcentral.com)
All cell lines examined displayed suprisingly low degrees of mGR on the surface area. (fabretp.org)
Furthermore, the mGR seems to have a home in caveolae and its own association with caveolin-1 (Cav-1) was obviously recognized in two from the four cell lines looked into using double reputation closeness ligation assay. (fabretp.org)
On the one hand it could be shown that primary cells are more susceptible to plasma treatment than the respective cell lines. (uni-greifswald.de)

In contrast, the changes in P21, P53, and BCL-2 genes differed in PBMCs and HDF cells. (celljournal.org)

caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death. (enzolifesciences.com)

Given that OKSM (Yamanaka) factors convert somatic cells into induced pluripotent stem (iPS) cells, alterations in transcriptional state could affect destiny of the cells. (intechopen.com)

The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)
The data support the broad value of CD20 as safety switch in adoptive T-cell therapy. (haematologica.org)
5 - 8 A drawback of TCR gene therapy is the formation of mixed TCR dimers, consisting of transgenic and endogenous TCR α and β chains. (haematologica.org)
Co-transfer of a suicide gene would provide a desirable safety switch in clinical TCR gene therapy. (haematologica.org)
In this study, we investigated the use of CD20 as safety switch after retroviral transfer to T cells with different cytomegalovirus (CMV) specificities, and conclude that CD20 may be broadly applicable as safeguard in adoptive T-cell therapy. (haematologica.org)
We're looking forward to hearing the latest advancement in cell and gene therapy and understanding current hurdles around clinical trials and commercialization. (kactusbio.com)
Our team hopes to offers solutions for cell and gene therapy commercialization with our selection of GMP-Grade and GMP-Ready enzymes as well as AAV ELISA Kits for AAV titer quantification. (kactusbio.com)

This binding results in the activation have been implicated with streptococcal toxic shock syn- of a large proportion of antigen-presenting cells and T drome (STSS). (cdc.gov)

Our screen was able to identify novel genes that are essential for the survival of primary T cells and genes that modulate the response of the T cells to the small molecule inhibitor treatment. (horizondiscovery.com)

Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR. lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background. (hindawi.com)

PMID 17216583 ] The functional haplotype of peptidylarginine deiminase IV (S55G, A82V and A112G) associated with susceptibility to rheumatoid arthritis dominates apoptosis of acute T leukemia Jurkat cells. (snpedia.com)

Additionally, we offer a Cas9 Nuclease ELISA kit for detection of residual Cas9 in edited cells. (kactusbio.com)

The foundation as well as the function of the GR isoform had been further looked into in the S-49 mouse T-lymphoma cell range (13C18). (fabretp.org)

Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. (nih.gov)

Nevertheless, in rats a GR immunoreactive proteins was detected for the plasma membrane of liver organ cells (10), of hippocampal and hypothalamic neurons (11), and of glial and neuronal cells Pyrantel pamoate in the lateral amygdala. (fabretp.org)

We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
Next to its well-established anti-bacterial properties, plasma can have lethal as well as stimulating effects on mammalian cells. (uni-greifswald.de)

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Vaccine-induced antibody and T cell responses correlated with protection against lethal influenza virus infection. (researchsquare.com)
Protective anti-influenza immunity often correlates with antibody responses to influenza surface glycoproteins, particularly hemagglutinin (HA), the main antigenic determinant on the surface of both influenza virus and infected cells. (researchsquare.com)

Anatomy15

Organisms3

Diseases4

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes21

Information Science3

MRNA8

Expression38

Proliferation9

Receptor13

Monocytes5

Protein18

Target genes2

Transcription10

Vitro1

Mutation3

Blot1

HeLa2

Regulation3

Hematopoietic7

Malignant6

Periodic Paralysis2

CRISPR-Cas92

Flow11

Lysate1

Induce4

Human20

Differentiation4

Knockout1

Regulate1

Kinase1

Microarray1

Inhibition2

Helper3

Molecules2

Marker3

Genome-wide1

Peripheral3

Tissue4

Macrophages1

Incubation1

Methods2

Conventional1

Analysis5

Differential1

Toxicity2

Lines6

Contrast1

Apoptotic1

Induced plur1

Therapy7

Antigen-prese1

Survival1

Line1

PMID1

ELISA1

Lymphoma cell1

Transcript1

Neuronal cells1

Mammalian cells2

Antibody3