• antigens
  • Some groups have cross-reactive gag antigens (e.g., the ovine, caprine, and feline lentiviruses). (wikipedia.org)
  • Antibodies to gag antigens in lions and other large felids indicate the existence of other as yet unidentified viruses related to feline lentivirus and the ovine/caprine lentiviruses. (wikipedia.org)
  • plasma membrane
  • Inhibition was associated with diffuse cytoplasmic staining of HIV Gag rather than accumulation at the plasma membrane, suggesting TRIM22 disrupts proper trafficking. (nih.gov)
  • B) Model illustrating how HIV Gag mimics the Tsg101-recruiting function of Hrs and redirects Tsg101 and the ESCRT-I complex to the plasma membrane to facilitate viral budding. (nih.gov)
  • HIV Gag is then tightly bound to the membrane surface via three interactions: 1) that between the MA HBR and the PI(4,5)P2 inositol phosphate, 2) that between the extruded myristoyl tail of MA and the hydrophobic interior of the plasma membrane, and 3) that between the PI(4,5)P2 arachidonic acid moiety and the hydrophobic channel along the MA surface. (wikipedia.org)
  • genome
  • Single genome amplification characterized the infections of 2 unprotected animals in the gag-env immunized group, both of which had reduced acute plasma viral loads that ended as transient infections indicating partial immune control. (nih.gov)
  • Human CHST7 genome location and CHST7 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • The RSV genome has terminal repeats enabling its integration into the host genome and also overexpression of RSV genes. (wikipedia.org)
  • The src gene was taken up by RSV and incorporated into its genome conferring it with the advantage of being able to stimulate uncontrolled mitosis of host cells, providing abundant cells for fresh infection. (wikipedia.org)
  • residues
  • A group of 41 peptides, each 24 amino acids long and overlapping with each other by 12 residues spanning the total gag open reading frame (orf) of HIV-1 (HTLV-IIIBH 10 isolate) were synthesized using Fmoc chemistry. (uni-regensburg.de)
  • Gag p24 (residues 261-269, GEIYKRWII) was used as a control HLA-B8 restricted peptide. (nih.gov)
  • Once Gag is translated on ribosomes, Gag polyproteins are myristoylated at their N-terminal glycine residues by N-myristoyltransferase 1. (wikipedia.org)
  • particles
  • Further studies showed that TRIM22 inhibited budding of virus-like particles containing Gag only, indicating that Gag was the target of TRIM22. (nih.gov)
  • Importantly, Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing native late domains.These observations indicate that Hrs normally functions to recruit Tsg101 to the endosomal membrane.HIV-1 Gag apparently mimics this Hrs activity, and thereby usurps Tsg101 and other components of the MVB vesicle fission machinery to facilitate viral budding. (nih.gov)
  • Importantly, Hrs222-777 can recruit Tsg101 and rescue the budding of virus-like Gag particles that are missing native late domains. (nih.gov)
  • binds
  • A conserved P(S/T)AP tetrapeptide motif within Gag (the "late domain") binds directly to the NH2-terminal ubiquitin E2 variant (UEV) domain of Tsg101. (nih.gov)
  • Thus TRIM22 binds specifically to HIV Gag. (nih.gov)
  • Their binding of PSG can be affected by heparin, which is a competitive inhibitor that binds to the GAG portion of a PG. (wikipedia.org)
  • capsid
  • These cleaved products include the matrix (MA), capsid (CA), and nucleocapsid (NC), which are able to enter other pathways to infect new cells. (wikipedia.org)
  • Antibodies
  • Precipitated Gag and TRIM22 were detected by Western blotting using p24CA or FLAG antibodies. (nih.gov)
  • TRIM22
  • We used transcriptional profiling to identify cellular genes that were induced by interferon treatment and identified TRIM22 as one of the most strongly up-regulated genes. (nih.gov)
  • Gene chip data on TRIM22 induction was confirmed using Northern blot analysis (Figure 1A). (nih.gov)
  • TRIM22 interacts with Gag.HOS-CD4/CXCR4 cells were co-transfected with pGag-GFP and pFLAG-TRIM22 (or empty vector control pFLAG) and immunoprecipitated with anti-GFP or anti-FLAG. (nih.gov)
  • Reverse immunoprecipitation using anti-FLAG pulled down Gag-GFP only when FLAG-TRIM22 was co-expressed. (nih.gov)
  • Treatment of the samples with RNaseA prior to immunoprecipitation did not interfere with the association of TRIM22 with Gag, indicating that an RNA bridge did not mediate the interaction. (nih.gov)
  • CXCR4
  • Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag. (nih.gov)
  • Expression of HIV-1 Gag attenuated downregulation of CXCR4, resulting in accumulation of undegraded receptors within intracellular compartments. (nih.gov)
  • gene's
  • In 2009, Mary Lou, an unwed mother from a previous relationship, became Gene's first mature love interest and in 2010 Gene offered Mary Lou his great-grandmother's ring. (wikipedia.org)
  • variants
  • Because there were only 2 infected animals in the gag-env vaccinated rhesus compared to 10 infected rhesus in the other 2 groups, the significance of finding single env variants in the gag-env vaccinated group could not be established. (nih.gov)
  • motif
  • The effect of Gag was dependent on an ESCRT-I interacting motif within the C-terminal p6 region of Gag. (nih.gov)
  • nucleotide
  • In the most common variant of sickle-cell disease, the 20th nucleotide of the gene for the beta chain of hemoglobin is altered from the codon GAG to GTG. (wikipedia.org)
  • shown to interact
  • Colony stimulating factor 1 receptor has been shown to interact with: Cbl gene, FYN, Grb2, Suppressor of cytokine signaling 1, This receptor is also linked with the cells of MPS. (wikipedia.org)
  • accessory
  • There are additional accessory genes depending on the virus (e.g., for HIV-1: vif, vpr, vpu, nef) whose products are involved in regulation of synthesis and processing viral RNA and other replicative functions. (wikipedia.org)
  • Sequence
  • The role of rev was further elucidated when it was found that Rev acts in trans to target a specific sequence present in the env gene of HIV-1 to initiate export of incompletely spliced HIV-1 RNA from the nucleus. (wikipedia.org)
  • LTR retrotransposons are further sub-classified into the Ty1-copia-like (Pseudoviridae), Ty3-gypsy-like (Metaviridae), and BEL-Pao-like groups based on both their degree of sequence similarity and the order of encoded gene products. (wikipedia.org)
  • expression
  • The regulated expression of each member of this gene family may be an important determinant of sulfated GAGs expression and the associated function of chondroitin sulfates as regulators of many biologic processes. (wikipedia.org)
  • Env expression is regulated by the gene product of rev. (wikipedia.org)
  • relatively
  • Following challenge with SIVsmE660, 5 of the 6 RhMs were infected in gag-env-GM-CSF group with relatively high PVLs that were comparable to the control group [Fig. 1C]. (nih.gov)