• mutations
  • The λ-red recombineering system was published in 1998 and allows for insertion, deletion, or mutations to E. coli genes. (wikipedia.org)
  • Humans born with mutations that deactivate the p53 gene suffer from Li-Fraumeni syndrome, a condition that dramatically increases the risk of developing bone cancers, breast cancer and blood cancers at an early age. (wikipedia.org)
  • vector
  • Cells that were transformed with a vector containing the neomycin resistance gene and the herpes tk+ gene are grown in a solution containing neomycin and Ganciclovir in order to select for the transformations that occurred via homologous recombination. (wikipedia.org)
  • In order to ensure growth of only transformed bacteria (which carry the desired plasmids to be harvested), a marker gene is used in the destination vector for selection. (wikipedia.org)
  • genetic
  • They say it is laying the technological foundation for a radically different 21st century health care system, in which drug companies will use genetic maps to find new drugs and doctors will screen for thousands of disease-causing genes. (sfgate.com)
  • Moreover, cells that do not integrate any of the genetic material test negative for both genes and therefore die as a result of poisoning with neomycin. (wikipedia.org)
  • 2002
  • This remained the main library building until it was moved to its current location at West 10th Avenue and Charnelton Street in 2002. (wikipedia.org)
  • diseases
  • Information obtained using sequencing allows researchers to identify changes in genes, associations with diseases and phenotypes, and identify potential drug targets. (wikipedia.org)
  • marker
  • In this case, a gene cassette with a dual selectable marker can be incorporated into the DNA at the specific location of mutagenesis. (wikipedia.org)
  • The embryonic stem cells that incorporated the knocked-out gene are isolated from the unaltered cells using the marker gene from step 1. (wikipedia.org)
  • So, for example, the "marker gene" could be for resistance to the antibiotic ampicillin. (wikipedia.org)
  • 1990
  • The Bethel Branch of Eugene Public Library is located at 1990 Echo Hollow Road. (krvm.org)
  • Eugene "Gene" Honda is a popular public address announcer for the Chicago White Sox (starting in 1985, full-time since 1991), Chicago Blackhawks (since 1990), DePaul University basketball, Big Ten Tournament, Illinois Fighting Illini football, and the NCAA Final Four (since 2010). (wikipedia.org)
  • bacteria
  • In addition to highly conserved primer binding sites, 16S rRNA gene sequences contain hypervariable regions that can provide species-specific signature sequences useful for identification of bacteria. (wikipedia.org)
  • While 16S hypervariable regions can vary dramatically between bacteria, the 16S gene as a whole maintains greater length homogeneity than its Eukaryotic counterpart, which can make alignments easier. (wikipedia.org)
  • synthetic biology
  • CAMBRIDGE, England--( BUSINESS WIRE )--Today, Monday 30th June, 2014, Isogenica, Cambridge UK and Imperial College London secure funding from the Technology Strategy Board (TSB), the UK's innovation agency, for a £347,000 project to optimise a gene library assembly technology for potential use in synthetic biology. (businesswire.com)
  • We are now interested in further optimising the technology for high throughput, automated manufacture of whole gene libraries and we believe that this could represent a powerful and valuable tool for commercialisation in the synthetic biology arena" said Kevin Mathews, CEO of Isogenica. (businesswire.com)
  • molecular
  • In a report in the current online issue of the journal Nature Methods, Dr. Hugo Bellen (http://flypush.imgen.bcm.tmc.edu/lab/), a professor of molecular and human genetics at BCM and a Howard Hughes Medical Institute investigator, and his colleagues describe the new libraries. (innovations-report.com)
  • It is suggested that 16S rRNA gene can be used as a reliable molecular clock because 16S rRNA sequences from distantly related bacterial lineages are shown to have similar functionalities. (wikipedia.org)
  • researchers
  • A knockout mouse or knock-out mouse is a genetically modified mouse (Mus musculus) in which researchers have inactivated, or "knocked out", an existing gene by replacing it or disrupting it with an artificial piece of DNA. (wikipedia.org)
  • By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
  • Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)
  • recombination
  • citation needed] At its inception, SCOPE was developed as a homology-independent recombination technique to enable the creation of multiple crossover libraries from distantly related genes. (wikipedia.org)
  • University
  • University of New Mexico, University Libraries, Center for Southwest Research. (wikipedia.org)
  • The Eugene C. Eppley Administration Building is located on the University of Nebraska at Omaha north campus in Omaha, Nebraska. (wikipedia.org)
  • After attending an Omaha University football game with then-college president Milo Bail in 1949, Gene Eppley was asked what the growing university needed. (wikipedia.org)
  • The overall growth of the university in the years after the building's dedication is attributed to the opening of the library, as well. (wikipedia.org)
  • proteins
  • These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or distantly related parental sequences to identify functional novelty among the encoded proteins. (wikipedia.org)
  • contain
  • Such naïve libraries usually contain only antibodies with low affinity to the desired antigen, making it necessary to apply affinity maturation by random mutagenesis as an additional step. (wikipedia.org)
  • Some (hyper)thermophilic archaea (i.e. order Thermoproteales) contain 16S rRNA gene introns that are located in highly conserved regions and can impact the annealing of "universal" primers. (wikipedia.org)
  • recognition
  • Selected by W. H. Hutchinson, 1957 Recognition : the poems of Eugene Manlove Rhodes / illustrated by Martha Julian, 1997 "Cultural Resource Information System (CRIS)" (Searchable database). (wikipedia.org)
  • process
  • To create the corresponding library of genes, the breeding scheme of Gregor Mendel was adapted into a PCR strategy to selectively cross hybrid genes, a process of iterative inbreeding to create all possible combinations of coding segments with variable linkages. (wikipedia.org)
  • The process is similar, comprising gene libraries from immunized or naïve donors and display techniques for identification of the most specific antigens. (wikipedia.org)
  • full
  • While cheaper and allowing for deeper community coverage, Illumina sequencing only produces reads 75-150 base pairs long, and has no established protocol for reliably assembling the full gene in community samples. (wikipedia.org)
  • Many community studies select semi-conserved hypervariable regions like the V4 for this reason, as it can provide resolution at the phylum level as accurately as the full 16S gene. (wikipedia.org)
  • together
  • The bacteriophage λ-red operon consists of the exo, bet, and gam genes which, together, are responsible for recombineering. (wikipedia.org)
  • Together they had had five sons (Eugene being the youngest) and three daughters, but neither parent spent much time with the children: his father, a brave, unglamorous French soldier, spent much of his time away campaigning, while Olympia's passion for court intrigue meant the children received little attention from her. (wikipedia.org)
  • eBooks
  • Things like hits on library websites, use of Wi-Fi in libraries or digital checkouts of downloadable, digital media, eBooks and things like that," she says, "three things which I think Eugene would do really well in. (eugeneweekly.com)
  • product
  • Any insertion of DNA that occurred via random insertion will die because they test positive for both the neomycin resistance gene and the herpes tk+ gene, whose gene product reacts with Ganciclovir to produce a deadly toxin. (wikipedia.org)
  • Both the PCR product containing the mammalian gene with the new restriction sites and the destination plasmid are subjected to restriction digestion, and the digest products are purified by gel electrophoresis. (wikipedia.org)
  • construction
  • Their collaborators at Lawrence Berkeley National Laboratory, Drs. Roger Hoskins and Joseph Carlson, played a key role in the design, construction, and annotation of the libraries. (innovations-report.com)
  • High
  • Gene graduated from Chicago's Senn High School in 1972. (wikipedia.org)
  • Gene Honda also is a teacher for the After School Matters (ASM) Sports Broadcasting program at Curie High School for the past 10 years. (wikipedia.org)
  • years old
  • Now 19 years old, Eugene applied directly to Louis XIV for command of a company in French service, but the King-who had shown no compassion for Olympia's children since her disgrace-refused him out of hand. (wikipedia.org)
  • reading room
  • In 1898, a group of women founded the Fortnightly Club and opened a private reading room in a store building in downtown Eugene. (wikipedia.org)
  • sites
  • Open Library is an initiative of the Internet Archive , a 501(c)(3) non-profit, building a digital library of Internet sites and other cultural artifacts in digital form. (openlibrary.org)
  • contains
  • The bacterial 16S gene contains nine hypervariable regions (V1-V9) ranging from about 30-100 base pairs long that are involved in the secondary structure of the small ribosomal subunit. (wikipedia.org)
  • approximately
  • The number of users of the library approximately tripled after the new building opened, and then further increased following the economic downturn of 2008. (wikipedia.org)
  • selection
  • In addition, a second gene, such as herpes tk+, is also included in the construct in order to accomplish a complete selection. (wikipedia.org)