• metabolism
  • In the presence of toluene, menadione reduced TodS activity whereas toluene did not stimulate activity in the presence of menadione.The opposing effects of menadione on glucose and toluene metabolism may be partially responsible for the interwoven regulation of both catabolic pathways.This work provides mechanistic detail on how complex sensor kinases integrate different types of signal molecules. (nih.gov)
  • mRNA expression
  • JNK1, JNK2 and JNK3 mRNA expression levels were quantified by real-time PCR. (biomedsearch.com)
  • Treatment of cells with siRNA resulted in a dose-dependent decrease in kallikrein mRNA levels, which impacted on other components of the kallikrein-kinin system, dose-dependently reducing bradykinin B2 receptor mRNA expression. (biomedsearch.com)
  • d,e) Embryos were injected with 30 ng skp2 Mo (d) or 30 ng Con Mo (e) in one blastomere at the two cell stage, along with ßgal mRNA as a lineage tracer, and analyzed for nßt mRNA expression at stage 15 ((d) arrow to show expansion of primary neurons). (nih.gov)
  • Mice
  • Examination of beta-galactosidase expression in heart, lung, kidney, liver, spleen, and brain of adult mice demonstrated robust signal in large and medium-sized blood vessels. (biomedsearch.com)
  • Western blotting of microdissected kidney nephron segments confirmed that expression of the thiazide-sensitive NCC (Na⁺-Cl⁻ co-transporter) was increased, as expected, in the distal convoluted tubule when transgenic mice were induced with doxycycline. (biomedsearch.com)
  • This inducible model shows, for the first time, that in vivo expression of a mutant WNK4 protein is sufficient to cause the rapid and reversible appearance of a PHAII disease phenotype in mice. (biomedsearch.com)
  • Furthermore, kidney arginase-2 expression increased in Ins2(Akita) mice compared with control. (nih.gov)
  • Molecular
  • To investigate this molecular mechanism, the protein synthesis of two subunits of Rubisco was characterized in transgenic rice (Oryza sativa) plants with overexpression or antisense suppression of the RBCS gene. (biomedsearch.com)
  • induction
  • These findings indicate that arginase-2 plays a major role in induction of diabetic renal injury and that blocking arginase-2 activity or expression could be a novel therapeutic approach for treatment of DN. (nih.gov)
  • Enzyme induction is a process in which a molecule (e.g. a drug) induces (i.e. initiates or enhances) the expression of an enzyme. (wikipedia.org)
  • effects
  • Finally, "in vivo" effects of S6K suppression were analyzed in the permanent middle cerebral artery occlusion model of ischemia , in which absence of S6K expression increased mortality and infarct volume. (curehunter.com)
  • inhibits
  • OGD-induced apoptosis was increased by the combined deletion of S6K1 and S6K2 genes, as well as by treatment with rapamycin that inhibits S6K1 activity by acting on the upstream regulator mTOR (mammalian target of rapamycin ). (curehunter.com)
  • In contrast, over-expression of skp2 inhibits neurogenesis in a manner dependent on its ability to act as part of the SCFskp2 complex. (nih.gov)
  • enzyme
  • Alternatively, the enzyme is recruited in response to the stimulus of gene activation (Adelman et al. (nih.gov)
  • Enzyme inhibition can refer to the inhibition of the expression of the enzyme by another molecule interference at the enzyme-level, basically with how the enzyme works. (wikipedia.org)
  • demonstrate
  • Using Xenopus as a model system, here we demonstrate that skp2 has an additional role in regulation of differentiation of primary neurons, the first neurons to differentiate in the neural plate. (nih.gov)
  • major
  • The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes. (biomedsearch.com)
  • cell
  • Xenopus skp2 shows a dynamic expression pattern in early embryonic neural tissue and depletion of skp2 results in generation of extra primary neurons.We show that SCFskp2 degrades Xic1 in embryos and this contributes to the ability of skp2 to regulate neurogenesis.We conclude that the SCFskp2 complex has functions in the control of neuronal differentiation additional to its role in cell cycle regulation. (nih.gov)
  • While its role in regulation of cell division has been well documented, additional functions in differentiation, including in the nervous system, have not been investigated. (nih.gov)
  • Moreover, inhibition of neurogenesis by skp2 occurs upstream of the proneural gene encoding NeuroD and prior to cell cycle exit. (nih.gov)
  • We conclude that the SCFskp2 complex has functions in the control of neuronal differentiation additional to its role in cell cycle regulation. (nih.gov)
  • mechanisms
  • Although regulation of Akt can be achieved through several mechanisms, including its phosphoinositide-binding Pleckstrin homology (PH) domain, how SGK kinases are targeted and regulated remains to be elucidated. (nih.gov)
  • levels
  • CONCLUSIONS: The regulation of ATP and adenosine levels by NTPDase and ADA activities may be important to preserve cellular integrity and to modulate the immune response in MS. (biomedsearch.com)
  • Rescue of either S6K1 or S6K2 expression by adenoviral infection revealed that protective functions were specifically mediated by S6K1, because this isoform selectively promoted resistance to OGD and reduction of ROS levels. (curehunter.com)
  • Role
  • The aim of the current investigation was to delineate the role of kallikrein in the regulation of fibrosis, by 'knocking down' its expression using specific small interfering RNAs (siRNA). (biomedsearch.com)
  • To determine 1) whether renal arginase activity or expression is increased in diabetes and 2) whether arginase plays a role in development of diabetic nephropathy (DN). (nih.gov)
  • early
  • Xenopus skp2 shows a dynamic expression pattern in early embryonic neural tissue and depletion of skp2 results in generation of extra primary neurons. (nih.gov)
  • Here we describe the cloning and expression of an early zebrafish melanoblast marker, dopachrome tautomerase. (zfin.org)
  • primary
  • Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. (cancerindex.org)
  • determine
  • 2009). To determine which situation applies to the macrophage Nos2 gene, we analyzed pol II association by ChIP. (nih.gov)