• Mice
  • Examination of beta-galactosidase expression in heart, lung, kidney, liver, spleen, and brain of adult mice demonstrated robust signal in large and medium-sized blood vessels. (biomedsearch.com)
  • Western blotting of microdissected kidney nephron segments confirmed that expression of the thiazide-sensitive NCC (Na⁺-Cl⁻ co-transporter) was increased, as expected, in the distal convoluted tubule when transgenic mice were induced with doxycycline. (biomedsearch.com)
  • This inducible model shows, for the first time, that in vivo expression of a mutant WNK4 protein is sufficient to cause the rapid and reversible appearance of a PHAII disease phenotype in mice. (biomedsearch.com)
  • mRNA expression
  • JNK1, JNK2 and JNK3 mRNA expression levels were quantified by real-time PCR. (biomedsearch.com)
  • Treatment of cells with siRNA resulted in a dose-dependent decrease in kallikrein mRNA levels, which impacted on other components of the kallikrein-kinin system, dose-dependently reducing bradykinin B2 receptor mRNA expression. (biomedsearch.com)
  • d,e) Embryos were injected with 30 ng skp2 Mo (d) or 30 ng Con Mo (e) in one blastomere at the two cell stage, along with ßgal mRNA as a lineage tracer, and analyzed for nßt mRNA expression at stage 15 ((d) arrow to show expansion of primary neurons). (nih.gov)
  • Vascular
  • Decreased CREB and β-catenin transcriptional activity restricts the aberrant transcription of key genes like EP4, cyclooxygenase (COX)-2, vascular endothelial growth factor and c-myc, which ultimately control cell survival, proliferation and angiogenesis. (cancerindex.org)
  • loci
  • These extended gene loci may harbor binding sites for nervous tissue-specific transcription factors. (nih.gov)
  • Whole gene loci, including promoters, UTRs, introns, and distant intergenic and spacer regions were included in this analysis. (nih.gov)
  • effects
  • Finally, "in vivo" effects of S6K suppression were analyzed in the permanent middle cerebral artery occlusion model of ischemia , in which absence of S6K expression increased mortality and infarct volume. (curehunter.com)
  • We present here the phenotypic effects of the ectopic expression of RNaseT2, a highly conserved ribonuclease encoded by chromosome 6q27, in SV40 immortalized cell lines. (nih.gov)
  • tissue
  • Xenopus skp2 shows a dynamic expression pattern in early embryonic neural tissue and depletion of skp2 results in generation of extra primary neurons.We show that SCFskp2 degrades Xic1 in embryos and this contributes to the ability of skp2 to regulate neurogenesis.We conclude that the SCFskp2 complex has functions in the control of neuronal differentiation additional to its role in cell cycle regulation. (nih.gov)
  • Xenopus skp2 shows a dynamic expression pattern in early embryonic neural tissue and depletion of skp2 results in generation of extra primary neurons. (nih.gov)
  • PK genes up-regulated in the highly proliferative testicular tissue are fast evolving and small, with short introns and transcribed regions. (nih.gov)
  • Structure and evolutionary divergence of tissue-specific PK genes is related to the proliferative activity of the tissue where these genes are predominantly expressed. (nih.gov)
  • Our data provide evidence that physiological requirements for transcription intensity, ubiquitous expression, and tissue-specific regulation shape gene structure and affect rates of evolution. (nih.gov)
  • Characteristic transcription factor binding sites over-represented in evolutionarily conserved regions of PK genes predominantly expressed in nervous tissue. (nih.gov)
  • PK genes up-regulated in the nervous tissue had significantly longer 5′-spacers and introns then genes down-regulated in the nervous tissue. (nih.gov)
  • To identify brain-specific regulatory elements, we analyzed conserved synteny regions of PK genes predominantly expressed in the nervous tissue with the DiRE program. (nih.gov)
  • Conserved synteny regions of PK genes with similar overall expression levels and low expression in the brain tissue were used as a background set. (nih.gov)
  • inhibits
  • OGD-induced apoptosis was increased by the combined deletion of S6K1 and S6K2 genes, as well as by treatment with rapamycin that inhibits S6K1 activity by acting on the upstream regulator mTOR (mammalian target of rapamycin ). (curehunter.com)
  • mutations
  • Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. (cancerindex.org)
  • CONCLUSIONS
  • CONCLUSIONS: The regulation of ATP and adenosine levels by NTPDase and ADA activities may be important to preserve cellular integrity and to modulate the immune response in MS. (biomedsearch.com)
  • levels
  • The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes. (biomedsearch.com)
  • Rescue of either S6K1 or S6K2 expression by adenoviral infection revealed that protective functions were specifically mediated by S6K1, because this isoform selectively promoted resistance to OGD and reduction of ROS levels. (curehunter.com)
  • The levels of transcripts involved in Akt signalling pathway, cell cycle control and pathways related to cell proliferation decreased 2-10-folds in SV40 immortalized cells in response to RNaseT2 expression. (nih.gov)
  • demonstrate
  • Using Xenopus as a model system, here we demonstrate that skp2 has an additional role in regulation of differentiation of primary neurons, the first neurons to differentiate in the neural plate. (nih.gov)
  • Role
  • The aim of the current investigation was to delineate the role of kallikrein in the regulation of fibrosis, by 'knocking down' its expression using specific small interfering RNAs (siRNA). (biomedsearch.com)
  • While its role in regulation of cell division has been well documented, additional functions in differentiation, including in the nervous system, have not been investigated. (nih.gov)
  • We conclude that the SCFskp2 complex has functions in the control of neuronal differentiation additional to its role in cell cycle regulation. (nih.gov)
  • cells
  • This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts. (nih.gov)
  • Although RNaseT2 expression is elevated in normal diploid fibroblasts approaching senescence (passage 64), forced expression of the gene in immortalized cells does not cause them to senesce. (nih.gov)
  • activity
  • The activity and expression of NTPDase is altered in lymphocytes of multiple sclerosis patients. (biomedsearch.com)
  • We evaluated the activity and expression of NTPDase and adenosine deaminase (ADA) activity in lymphocytes from patients with the relapsing-remitting form of MS (RRMS). (biomedsearch.com)
  • level
  • Relationship between the expression of IP-10 and IP-10 mRNA in peripheral blood and HBV DNA level in. (biomedsearch.com)
  • Enzyme inhibition can refer to the inhibition of the expression of the enzyme by another molecule interference at the enzyme-level, basically with how the enzyme works. (wikipedia.org)
  • early
  • Here we describe the cloning and expression of an early zebrafish melanoblast marker, dopachrome tautomerase. (zfin.org)
  • small
  • Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome. (biomedsearch.com)
  • health
  • These transgenic eNOS promoter-reporter lines will be a valuable resource for ongoing studies addressing the regulated expression of eNOS in vivo in both health and disease. (biomedsearch.com)