Gene expression rna stability. Medical search. Frequent questions

Here, we explore the role of RNA stability in achieving balanced gene expression and present genome-wide RNA stabilities in Drosophila melanogaster male and female cells as well as male cells depleted of proteins essential for dosage compensation. (scilifelab.se)
Mechanically, m6A can be recognized by "reader" proteins or can directly modify RNA conformation, and it widely affects gene expression by mediating RNA stability, translation, splicing and export. (scienceopen.com)
The pleiotropic effects of both proteins are perfect examples of the importance of RNA decay and translation in maintaining correct cell functioning. (ens-lyon.fr)
The DNA that makes up genes contains the instructions to synthesise proteins, but it's wrong to think that, for a given gene, these instructions are always the same for all parts of the organisms . (evolutionnews.org)
Y-Complex Proteins Show RNA-De. (uni-marburg.de)
In living organisms, gene expression is a complex process that results in the production of proteins from the genome in a time- and space-regulated manner. (cea.fr)
This expression is strictly dependent on the synthesis of messenger RNAs transcripts from the DNA molecule and their subsequent translation into proteins by the ribosomes. (cea.fr)
As they are synthesized, mRNAs undergo chemical modifications that ensure their stability, their delivery to ribosomes and eventually their translation into proteins. (cea.fr)
In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)
The effect of miR-126 expression on target proteins was assessed using miR-126 mimics or miR-126 inhibitors in ESCC cell lines. (spandidos-publications.com)
DNA methyltransferases are not limited to catalyzing DNA methylation, but also take part in the regulation of gene expression through interactions with other proteins that repress transcription and modify chromatin structure. (caister.com)
Accurate regulation of messenger activity and stability are critical to determine the timing and the amount of the proteins that will be made and are essential for successful segmentation and embryonic survival. (dundee.ac.uk)
It will provide greater understanding of the mechanisms regulating the 'correct' amount of the messengers and proteins made by clock genes. (dundee.ac.uk)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. (thermofisher.com)
This type of RNA is called a messenger RNA ( mRNA ), as it serves as a messenger between DNA and the ribosomes, molecular machines that read mRNA sequences and use them to build proteins. (khanacademy.org)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)

A prerequisite for rapid adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA stability as well as in maturation. (uni-marburg.de)
The methods (RNA or codon optimization to increase mRNA stability transport and expression) had applications in both DNA and viral vaccine vectors and also in gene therapy protocols. (cancer.gov)
miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
Mature microRNAs (miRNAs) are a class of small, well-conserved, non-coding RNA molecules that silence gene expression usually by interfering with mRNA stability or protein translation ( 6 , 7 ). (spandidos-publications.com)
The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. (elifesciences.org)
Consequently, motif quality is a major determinant of mRNA stability for Puf3 targets in vivo and can be used for the prediction of mRNA targets. (elifesciences.org)

Proline-, glutamic acid- and leucine-rich protein 1 (PELP1) also known as modulator of non-genomic activity of estrogen receptor (MNAR) and transcription factor HMX3 is a protein that in humans is encoded by the PELP1 gene. (wikipedia.org)
PELP1 plays critical roles in 60S ribosomal subunit synthesis and ribosomal RNA transcription. (wikipedia.org)
The steady state levels of RNAs, often referred to as expression levels, result from a well-balanced combination of RNA transcription and decay. (scilifelab.se)
Alterations in RNA levels will therefore result from tight regulation of transcription rates, decay rates or both. (scilifelab.se)
The first of these responds to acute and global changes in transcription and thus counteracts potentially harmful gene mis-expression by shifting the RNA stability in the direction opposite to the transcriptional change. (scilifelab.se)
Noncoding RNAs (ncRNAs) are noncoding transcripts that take part in epigenetic mechanisms by providing RNA-directed silencing, aiding recruitment of chromatin modifying complexes and in some instances, presenting enhancer-like functions to boost transcription. (duke.edu)
Our aim is to define the molecular bases of the interactions that are established between chromatin and the components of the nuclear envelope, and determine how they 1) regulate epigenetic state of genes and their transcription, 2) participate in spatial organization of the genome, and 3) contribute to cellular functions during mammalian development. (duke.edu)
G-quadruplex (G4) DNA, an alternate structure formed by Hoogsteen hydrogen bonds between guanines in G-rich sequences, threatens genomic stability by perturbing normal DNA transactions including replication, repair, and transcription. (nih.gov)
For example, the transcription, translation, and degradation of an mRNA is crucial to any gene expression event, and all three processes are controlled by a combination of elements including promoters, ribosome binding sites (RBSs), and cis -regulatory signals encoded in untranslated regions (UTRs). (universityofcalifornia.edu)
In agreement with these observations, the Pcif1 protein is distributed all along the polytene chromosomes at the level of active transcription sites and interferes with chromatin-dependent gene expression regulation. (cea.fr)
Dr Philip Murray, co-investigator on the project explains, "Almost all the existing mathematical models of the segmentation clock describe how clock protein inhibits clock gene transcription via a negative feedback loop. (dundee.ac.uk)
We describe MAGICAL, a hierarchical Bayesian approach that leverages paired scRNA-seq and scATAC-seq data from different conditions to map disease-associated transcription factors, chromatin sites, and genes as regulatory circuits. (simonsfoundation.org)
Moreover, expression levels of DEGs were verified in HNSCC cells and tissues using reverse transcription‑quantitative (RT‑q)PCR. (spandidos-publications.com)
Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (nih.gov)
miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (nih.gov)
MicroRNAs (miRNAs) are small non-coding RNAs that modulate gene expression at the post-transcriptional level by affecting both the stability and translation of complementary mRNAs. (wjgnet.com)
Besides classical gene regulation, miRNAs have emerged as post-transcriptional regulators of angiogenesis. (plos.org)
It has been suggested that abnormal expression of microRNAs (miRNAs) is associated with carcinogenesis. (spandidos-publications.com)
Up to 30% of human protein-coding genes may be regulated by miRNAs ( 8 , 9 ). (spandidos-publications.com)
Still other RNA molecules, such as tiny microRNAs ( miRNAs ), act as regulators of other genes, and new types of non-protein-coding RNAs are being discovered all the time. (khanacademy.org)
MicroRNAs (miRNAs or miRs) are involved in the post-transcriptional regulation of gene expression via complete or partial base pairing with the 3′untranslated region of target genes ( 3 ). (spandidos-publications.com)

Although biochemical studies indicate that N(6)-methyladenosine (m(6)A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and functions has been impeded by a lack of robust analytical methods. (scienceopen.com)
Gas-Exchange and Ultrastructural Analysis of Transgenic Potato Plants Expressing a Messenger-Rna Antisense Construct Targeted to the Cp-Fbpase. (mpg.de)
When genes are activated, a copy is made of the desired gene: the messenger RNA, which then subsequently gets translated into the protein that is required. (dundee.ac.uk)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)

Our long-term goal is to delineate the molecular bases and order of genetic, epigenetic and cellular processes that link altered expression of long ncRNAs and pathways driven by these molecules to cancer. (duke.edu)
Small molecules, genes and complex traits. (mpg.de)
Many non-coding sequences produce RNA molecules that regulate gene expression by turning them on and off. (evolutionnews.org)
For instance, some genes specify ribosomal RNAs ( rRNAs ), which serve as structural components of ribosomes, or transfer RNAs ( tRNAs ), cloverleaf-shaped RNA molecules that bring amino acids to the ribosome for protein synthesis. (khanacademy.org)

Here we report that a minimal combination of cis- regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. (nature.com)
This stable silencing requires components of the small RNA pathway and can silence homologous sequences in trans . (nature.com)
The male sterilizing factors are produced by recombination of the mitochondrial genome and consist of unusual genes or open reading frames (ORFs) that usually contain a portion of functional mitochondrial genes and sequences of unknown origin ( Hanson and Bentolila, 2004 ). (frontiersin.org)
We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

He was one of the first researchers to discover ribosomal frameshifting as a gene expression mechanism in plant RNA viruses and to demonstrate recombination between transgene mRNA and genomic RNA of an infecting virus. (bio5.org)

Expression of Foreign Genes in Potato: Promoters, RNA-Stability and Protein Accumulation. (mpg.de)
Indeed, FANTOM5's survey of 95 percent of protein-coding genes found near universal coverage by promoters, based on the first few bases of RNA transcripts. (evolutionnews.org)
FANTOM5 extends the third and fourth FANTOM atlases by including 4,721 human and 5,127 mouse genes, focusing on primary cells, cell lines and tissues, but that's not all: "The atlas also detected signals from the promoters of short RNA primary transcripts, and long non-coding RNAs . (evolutionnews.org)
We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)

During evolution, this protein naturally lost its catalytic activity in Drosophila where Pcif1 is, like its human counterpart, expressed in the nucleus and associated with the C-terminal domain of RNA polymerase (RNA Pol II). (cea.fr)
This protein binds to the phosphorylated form of serine 5 of RNA polymerase II ( Figure ) and may directly modulate its activity or promote the recruitment of chromatin components. (cea.fr)
These results suggest a similar contribution of PCIF1 to the fine regulation of RNA polymerase II activity in mammals, in addition to its mRNA methylation activity, whose role in mRNA translation efficiency is actively studied but still controversial. (cea.fr)

PELP1 modulates the expression of miRs, PELP1-mediated epigenetic changes play important role in the regulation miR expression and many of PELP1 mediated miRS are involved in promoting metastasis. (wikipedia.org)
In the Yildirim lab, we study how epigenetic mechanisms, particularly those that are mediated by long noncoding RNAs (ncRNAs), complement gene expression, impact genome stability and define cell fate decisions. (duke.edu)
Our laboratory uses primarily XCI as a model to understand how long ncRNAs 1) regulate gene dosage and maintain epigenetic state, 2) impact genome stability, and 3) participate in cell fate decisions. (duke.edu)
Positive feedback alone, however, does not guarantee the stability of an epigenetic change across generations. (nature.com)
In mammals, cytosine methylation at CpG positions of the DNA sequence is one of the hallmarks of epigenetic gene silencing. (caister.com)
While differential expression analysis failed to show predictive value, MAGICAL identified epigenetic circuit biomarkers that distinguished MRSA from MSSA. (simonsfoundation.org)
orthogonal exPeriMental changes and pathways induced by EGE investigated the impact of aSSeSSMent of ePigenetic environmental factors in cancer causation, both current H. pylori infection and driver geneS and their link to underpinning studies of etiology e , pigenetic memory of past (eradicated) environMental carcinogenS carcinogen evaluation, and prevention. (who.int)

Studies carried out by scientists at IRIG, in collaboration with the University of Geneva, on the fruit fly model Drosophila melanogaster have revealed the role of Pcif1 in the control of gene expression, despite the fact that this protein has completely lost its RNA methyltransferase activity compared to its mammalian counterpart PCIF1. (cea.fr)
Researchers at IRIG, in collaboration with the University of Geneva, focused on a RNA mammalian methyltransferase, the PCIF1 protein (homologous to the Drosophila Pcif1 protein), which adds an extra methyl group to m 6 A (m 6 adenosine) to form m 6 Am when the first transcribed nucleotide is an adenosine. (cea.fr)
The work of the scientists shows that mutation of Pcif1 gene in Drosophila results in a deregulation of the expression of a set of genes, reduced body weight and a significant drop in fertility which indicates an important role for this protein in the physiology of the organism. (cea.fr)
The lack of catalytic activity of Pcif1 in Drosophila reveals a new mechanism of gene expression regulation. (cea.fr)

We identify over 12,000 m(6)A sites characterized by a typical consensus in the transcripts of more than 7,000 human genes. (scienceopen.com)

A transcriptional arrest leads to a strong change in localization and dynamics of YmcA, YlbF and YaaT, supporting their involvement in global RNA degradation. (uni-marburg.de)
1991 ) Thermal stability of turnip yellow mosaic virus RNA: effect of pH and multivalent cations on RNA deaggregation and degradation Bba - Gene Structure and Expression . (academictree.org)

Genetic systems often behave unpredictably due to structural interactions between DNA, RNA and protein components as well as functional interactions with host factors and metabolites. (universityofcalifornia.edu)
Some viruses use RNA, not DNA, as their genetic material, but aren't technically considered to be alive (since they cannot reproduce without help from a host). (khanacademy.org)
Genetic analysis found a 3-bp deletion in the covR gene of the HH/LC variant. (cdc.gov)
More than 20 different genetic mutations resulting in the functional deletion of both pairs of alpha-globin genes (--/--) have been identified. (medscape.com)
There are more than 15 different genetic mutations that result in decreased production of alpha globin, usually through functional deletion of 1 or more of the 4 alpha-globin genes. (medscape.com)
In rare cases, a deletion of genetic material near the beginning of the LMNB1 gene leading to increased production of lamin B1 causes ADLD. (medlineplus.gov)
In at least one family with ADLD, the condition is instead caused by a loss (deletion) of genetic material near the beginning of the gene. (medlineplus.gov)

Dr. Xiong research encompasses a wide range of subjects that include virus diversity, recombination, RNAi suppression, host resistance and resistance-breaking, replication and movement of RNA viruses, and how all these are related to the control and management of viral diseases. (bio5.org)
His recent research on Citrus tristeza virus has revealed its genome stability, unusual for an RNA virus, and the promiscuous recombination between viral strains as the major factor driving Citrus tristeza virus evolution. (bio5.org)
ADAR can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). (thermofisher.com)
Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). (thermofisher.com)
Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. (thermofisher.com)
Gene therapy involves viral, plasmid, and oligonucleotide-based approaches. (medscape.com)

We are interested in defining the molecular bases of these interactions and delineating their significance in driving gene expression and genome functions. (duke.edu)
To elucidate the role of FANCJ in genomic stability, its molecular functions in G4 metabolism were examined. (nih.gov)
Molecular characterization of CNOT1 variants revealed normal CNOT1 expression levels, with both mutant and wild-type alleles expressed at similar levels. (bvsalud.org)
This progression from DNA to RNA to protein is called the " central dogma " of molecular biology. (khanacademy.org)
Circular RNAs (circRNAs) are molecular sponges that are involved in regulation of multiple types of cancer. (spandidos-publications.com)

The C-terminal domain of YTHDF2 selectively binds to m6A-containing mRNA whereas the N-terminal domain is responsible for the localization of the YTHDF2-mRNA complex to cellular RNA decay sites. (scienceopen.com)
In the past, we have been working on two cellular factors, the translation initiation factor INT6/EIF3E and the RNA helicase UPF1, that act at the crossroads of RNA translation and decay. (ens-lyon.fr)
Yet traditional genome-wide approaches fail to address complex patterns of gene expression, which include both synthesis and decay. (escholarship.org)
Although candidate studies have revealed important mechanisms of post-transcriptional regulation of gene expression via mRNA decay, there is a lack of global information on mRNA decay during development. (escholarship.org)
Decreased expression of the RNA-binding protein, Pumilio, stabilizes predicted neural mRNA targets and presence of a 3'UTR PRE is sufficient to trigger mRNA decay in the nervous system. (escholarship.org)
This work demonstrates a dynamic post-transcriptional program including mRNA decay allows fine-tuning of gene expression during neural development. (escholarship.org)

The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (nih.gov)
This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)

This allows the cell to switch networks of related genes off together without affecting the mRNAs it still needs. (elifesciences.org)

Regulation of RNA stability and translation are two key steps of cellular homeostasis. (ens-lyon.fr)
This includes gene, protein and metabolic networks, cellular architecture and intracellular dynamics, cell communication and motility, cell division and differentiation, tissue formation and organogenesis, tissue and organ functions, changes in population characteristics as a consequence of interaction of organisms with their physical environment, with individuals of their own species, and with organisms of other species. (nih.gov)
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. (biomedcentral.com)
Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). (thermofisher.com)
Therapeutic strategies for the dystrophinopathies can be categorized into 3 groups based on their approach: (1) Supportive pharmacologic therapy, (2) research gene therapy, and (3) research cellular therapy. (medscape.com)

Due to the development of RNA sequencing technology, the distribution pattern of m6A in the transcriptome has been uncovered. (scienceopen.com)
With the increasing application of high-throughput sequencing, transcriptome analysis has become an effective method for identifying novel stable reference genes. (biomedcentral.com)
In this study, we identified candidate reference genes based on transcriptome data covering embryos and larvae of early development, normal adult tissues, and the hindgut under sulfide stress using the coefficient of variation (CV) method in the echiuran Urechis unicinctus , resulting in 6834 (15.82%), 7110 (16.85%) and 13880 (35.87%) candidate reference genes, respectively. (biomedcentral.com)
Our study is the first to select reference genes for RT-qPCR from transcriptome data in Echiura and provides important information for future gene expression studies in U. unicinctus . (biomedcentral.com)
With the increasing application of high-throughput sequencing, RNA-seq has provided a new strategy for identifying new highly stable reference genes from transcriptome data. (biomedcentral.com)
Moreover, results from bulk transcriptome sequencing (RNA-seq) and SCT experiments revealed distinct transcriptomic signatures representing cell injury and stress in primary RPTE in contrast to the uncultured, freshly dissociated PT from human kidney. (simonsfoundation.org)

Both mechanisms are global, act on housekeeping as well as non-housekeeping genes and were observed in both flies and mammals. (scilifelab.se)
Regulatory mechanisms for chromosomal genes encoding multidrug resistance (MDR) efflux pumps (EPs) in Staphylococcus aureus are poorly defined. (manchester.ac.uk)

In various organisms, components of the nuclear envelope interact with chromatin that is spatially organized within the nucleus (Fig 2) and subsequently regulate gene expression. (duke.edu)
Resolving chromatin remodeling-linked gene expression changes at cell type resolution is important for understanding disease states. (simonsfoundation.org)

Recovery is also observed in most cases when double-stranded RNA is used to silence the same coding sequence in different regulatory contexts that drive germline expression. (nature.com)
Methods and/or tools to facilitate the combination of various regulatory elements originating from various different sources to predictably control the activity of any desired RNA would be beneficial for numerous biotechnology applications. (universityofcalifornia.edu)
However, regulatory elements can unpredictably interact with each other through the formation of RNA structures and the recruitment of factors that affect global transcript accessibility and stability. (universityofcalifornia.edu)
The invention allows for the combination of multiple regulatory elements in a fashion that predictably affects RNA activity. (universityofcalifornia.edu)
The project aims to identify the regulatory processes governing regulation of messenger activity and stability of segmentation clock genes. (dundee.ac.uk)
It is thought that this deletion removes a regulatory region of DNA that helps control the expression of the LMNB1 gene. (medlineplus.gov)

Microbiological and gene expression data correlated in four instances, existing knowledge of the substrate specificity of NorC resulted in correlation in two others, and a transcriptional/translational disconnect is possible for the remaining two. (manchester.ac.uk)
the CRISPR-associated nuclease Cas9 (SpCas9), a specificity-determining CRISPR RNA (crRNA), and an auxiliary trans-activating crRNA (tracrRNA) 11 . (cdc.gov)

The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. (wiley-vch.de)
The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. (wiley-vch.de)
Before this information can be used for protein synthesis, however, an RNA copy (transcript) of the gene must first be made. (khanacademy.org)
Genes that regulate both the synthesis and the structure of different globins are organized into 2 separate clusters. (medscape.com)
Alpha thalassemia syndromes are caused by deficient expression of 1 or more of the 4 alpha-globin genes on chromosome 16 and are characterized by absent or reduced synthesis of alpha-globin chains. (medscape.com)

Silencing the m(6)A methyltransferase significantly affects gene expression and alternative splicing patterns, resulting in modulation of the p53 (also known as TP53) signalling pathway and apoptosis. (scienceopen.com)

During evolution, CpG rich regions, so-called CpG islands, have been established as prominent features of promoter regions of genes. (caister.com)

Selecting suitable reference genes is extremely important for the accuracy of RT-qPCR results. (biomedcentral.com)
Commonly used reference genes are not always stable in various organisms or under different environmental conditions. (biomedcentral.com)
Subsequently, ten genes including five new candidate reference genes and five commonly used reference genes, were validated by RT-qPCR. (biomedcentral.com)
The comprehensive results indicated that the new candidate reference genes were more stable than most commonly used reference genes. (biomedcentral.com)
The candidate reference genes STX12 , EHMT1 , and LYAG were the most stable genes in early development, normal adult tissues, and hindgut under sulfide stress, respectively. (biomedcentral.com)
Reference genes are used for the normalization of gene expression because of the stability of expression levels among different tissues, different developmental stages, or under various treatments [ 5 ]. (biomedcentral.com)
Therefore, two or more reference genes are desirable to improve the reliability and accuracy of the RT-qPCR results. (biomedcentral.com)

Subsequently, immune-related gene expression was profiled using the PanCancer Immune Profiling Panel of NanoString technology. (eur.nl)
Mutations and gene deletions causing the thalassemia genotype have arisen independently in different populations but have subsequently propagated by means of natural selection. (medscape.com)

This could reveal genes that work together, helping to unravel their roles inside cells. (elifesciences.org)

Mechanistic studies showed that PELP1 interaction with the arginine methyltransferase PRMT6 plays a role in RNA splicing. (wikipedia.org)

It has been reported that miR-126 is located on chromosome 9q34.3 within the host gene encoding for epidermal growth factor like-7 (EGFL-7), an endothelial cell-derived, secreted inhibitor of smooth muscle cell migration and a regulator of blood vessel formation ( 12 , 13 ). (spandidos-publications.com)
A chromosome may contain tens of thousands of genes , each providing instructions on how to make a particular product needed by the cell. (khanacademy.org)
The alpha-globin genes are encoded on chromosome 16, and the gamma-, delta-, and beta-globin genes are encoded on chromosome 11. (medscape.com)
Alpha-chain genes in duplication on chromosome 16 pairing with non-alpha chains to produce various normal hemoglobins. (medscape.com)

Modulation of RNA stability regulates gene expression in two opposite ways: through buffering of RNA levels upon global perturbations and by supporting adapted differential expression. (scilifelab.se)
The second response enhances inter-individual differential gene expression by adjusting the RNA stability in the same direction as a transcriptional change. (scilifelab.se)
The most common approach in transcriptomics (RNA-seq and microarrays) is differential gene expression analysis (DGEA). (mdpi.com)
This Special Issue includes 12 research articles and one review on the topic of differential gene expression and coexpression. (mdpi.com)
While differential gene expression largely returned to baseline levels after the virus became undetectable, some differentially methylated sites persisted for months of follow-up, with a pattern resembling autoimmune or inflammatory disease. (simonsfoundation.org)
However, as the present case will illustrate, selective pressures in vivo may cause differential expression of certain GBS surface components during colonization or dissemination. (cdc.gov)

However recent works clearly demonstrate that RNA stability per-se is critical for cell homeostasis. (ens-lyon.fr)
Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)

however, restoration of CBY1 expression in tumor cells reduces BCSC and its enrichment, thus lnc408 plays an essential role in maintenance of BCSC stemness. (nature.com)
The expression of miR-126 was significantly lower in ESCC tissues, which was associated with tumor differentiation, lymph node metastasis, tumor in-depth and TNM stage. (spandidos-publications.com)
FUT6 and CGNL1 were downregulated in HNSCC compared with adjacent healthy tissue and the expression levels of these genes were associated with tumor stage. (spandidos-publications.com)

These findings suggest FANCJ is a specialized Fe-S cluster helicase that preserves chromosomal stability by unwinding unimolecular G4 DNA likely to form in transiently unwound single-stranded genomic regions. (nih.gov)
The hnRNP protein has a strong affinity for polypyrimidine-rich RNA and for single-stranded polypyrimidine-rich DNA. (bvsalud.org)

PELP1 functions as a coactivator of several NRs and regulates genes involved in proliferation and cancer progression. (wikipedia.org)
PELP1 also interacts with MTp53, regulates its recruitment, and alters MTp53 target gene expression. (wikipedia.org)

Regulation of mRNA translation is another important aspect of the posttranscriptional control of gene expression. (ens-lyon.fr)
To gain insights into the relationship between genotype, phenotype and disease, FMI scientists work to understand genome output by molecularly defining the control of gene expression and of RNA and protein stability. (fmi.ch)

CNOT1 is a member of the CCR4-NOT complex, which is a master regulator, orchestrating gene expression , RNA deadenylation, and protein ubiquitination . (bvsalud.org)
Here, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs) and Pumilio-response elements (PREs). (elifesciences.org)
Shortening or removal of mRNA poly(A) tails (deadenylation) represses gene expression in eukaryotes. (elifesciences.org)

Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)

Besides its role in the regulation of genes, DNA methylation silences repetitive elements and appears to be important for the stability of the mammalian genome. (caister.com)

Differentially expressed genes (DEGs) may be responsible for phenotypic differences between various biological conditions. (mdpi.com)
Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed genes (DEGs). (spandidos-publications.com)

While Xist expression is crucial for initiation of XCI, its role in maintenance of XCI is not clear. (duke.edu)

and facilitates activation of genes involved in proliferation and cancer progression. (wikipedia.org)
A novel potential application for cancer is DNA-delivered immunotherapies, based on the development of efficient expression vectors for cytokines and other immunomodulators. (cancer.gov)
Studies have shown that the expression of miR-126 is downregulated in cancers such as non-small cell lung cancer and colon cancer ( 14 , 15 ), suggesting that the downregulation of miR-126 is significantly related to the occurrence and development of cancer. (spandidos-publications.com)

Genes are the "code" for building the biological elements that form an organism. (evolutionnews.org)
An alternative approach is gene coexpression analysis, which detects groups of genes with similar expression patterns across unrelated sets of transcriptomic data from the same organism. (mdpi.com)
This study highlights an important contribution for Pcif1 in gene expression and physiology of the organism independently of any catalytic activity. (cea.fr)

We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
Impairs HCV replication via RNA editing at multiple sites. (thermofisher.com)
The protein also plays a role in the copying (replication) of DNA in preparation for cell division and the activity (expression) of many genes by being involved in the organization of chromosomes within the nucleus. (medlineplus.gov)

De Novo Variants in CNOT1, a Central Component of the CCR4-NOT Complex Involved in Gene Expression and RNA and Protein Stability, Cause Neurodevelopmental Delay. (bvsalud.org)
Conversely, the HH and LH variants exhibited low capsule (LC) and high capsule (HC) expression, respectively. (cdc.gov)

Additionally, we show that, in contrast to mammals, modulation of RNA stability does not detectably contribute to dosage compensation of the sex-chromosomes in D. melanogaster. (scilifelab.se)

Post-transcriptional regulation is essential for temporal and spatial control of protein expression. (escholarship.org)

Previously, we showed that DNA vaccine expression in the tissues is a major limiting step for meaningful immunogenicity and we developed general methods for efficient plasmid expression. (cancer.gov)

and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. (thermofisher.com)

ART exhibited significant anti-angiogenic role on granulomas illustrated by remarkable milder intensity and significantly lower expression values of VEGF and CD34 immunostaining compared to PZQ and non-treated groups. (degruyter.com)
In addition, 192/730 genes showed significantly higher gene counts in Tempus samples, 157/730 genes showed higher gene counts in PBMCs. (eur.nl)

In addition to the RNA helicase UPF1, we develop an axis of research based on the G4 resolvase DHX36 (project 2) . (ens-lyon.fr)

PELP1 binds RNA, and participates in RNA splicing. (wikipedia.org)

GBS production of β-h/c is encoded by the genes of the cyl operon ( 5 , 6 ) and is associated with direct lysis of a variety of eukaryotic cell types ( 7 - 9 ), inflammatory activation ( 10 - 12 ), and virulence in animal models ( 10 , 13 , 14 ). (cdc.gov)

FANCJ belongs to a conserved iron-sulfur (Fe S) cluster family of helicases important for genomic stability including XPD (nucleotide excision repair), DDX11 (sister chromatid cohesion), and RTEL (telomere metabolism), genetically linked to xeroderma pigmentosum/Cockayne syndrome, Warsaw breakage syndrome, and dyskeratosis congenita, respectively. (nih.gov)

The fly Pcif1 expressed in the nucleus and binds the phosphorylated C-terminal domain (CTD) of RNA Pol II (at the level of phosph orylated s erine 5). (cea.fr)

An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. (scienceopen.com)
with analyses of structural variation, gene modeling and transcriptomes. (bio5.org)

Anatomy14

Organisms15

Diseases2

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes107

Disciplines and Occupations3

Information Science8

Health Care4

Proteins18

MRNA stability6

Transcription14

MiRNAs8

Messenger RNA4

Molecules4

Sequences4

Ribosomal1

Promoters4

Polymerase3

Epigenetic7

Drosophila4

Transcripts1

Degradation2

Genetic7

Viral6

Molecular5

Decay6

MiRNA2

MRNAs1

Cellular5

Transcriptome6

Mechanisms2

Chromatin2

Regulatory6

Specificity2

Synthesis5

Signalling pathway1

Promoter Regions1

Reference Genes7

Subsequently2

Roles1

Methyltransferase1

Chromosome4

Differential6

Demonstrate2

Tumor3

Single-stranded2

Regulates2

Control of gene expression2

Deadenylation3

Enhances1

Methylation1

Differentially2

Initiation1

Cancer3

Organism3

Replication3

Variants2

Mammals1

Transcriptional1

Plasmid1

Interactions1

Significantly2

Helicase1

Splicing1

Eukaryotic1

Genomic stability1

Binds1

Structural2