Gene expression wnt4 protein. Medical search. Frequent questions

WNT4 drives a novel signaling pathway in ILC cells, with a critical role in estrogen-induced growth that may also mediate endocrine resistance. (nih.gov)
In the mouse uterus, DES altered the expression of chromatin-modifying proteins and Wnt signaling pathway members, caused epigenetic changes in the sine oculis homeobox 1 gene , and decreased the expression of angiogenic factors. (diethylstilbestrol.co.uk)
This mutant, which we called boygirl ( byg ), was shown to contain an early stop codon that disrupts the autosomal gene encoding MAP3K4, a component of the mitogen-activated protein kinase (MAPK) signaling pathway. (plos.org)
Although the etiology of dental agenesis The association between AXIN2 and CRC involves defects in the involved genetic and environmental factors, the genes more canonical WNT signaling pathway, which regulates and coordi- frequently associated with hypodontia in different populations are nates the AXIN complex for the degradation of β-catenin under 2,3 AXIN2, MSX1, PAX9, EDA, and WNT10. (sagepub.com)

Wnt4 - wingless-type MMTV integration site family. (wikigenes.org)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 4 (WNT4) were tested 20 times on one plate, respectively. (biomatik.com)
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 4 (WNT4) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 4 (WNT4). (biomatik.com)
No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 4 (WNT4) and analogues was observed. (biomatik.com)

One potential novel pathway is via the Wnt ligand WNT4, a critical signaling molecule in mammary gland development regulated by the progesterone receptor. (nih.gov)
The down-regulation was attributed to a lowered expression of Wnt4, a necessary ligand within the activation stages from the Wnt pathway, within the mammary cells of parous mice [56]. (scdinhibitor.com)
Stimulation with exogenous FGF boosts the expression of endogenous Fgf genes while repressing Bmp ligand genes. (biologists.com)

One important example is the stabilization of β catenin, which increases the expression of target genes. (wikipedia.org)
Consequently, cytosolic β-catenin is relieved from constitutive proteasomal degradation and induces the transcription of target genes through association with TCF/LEF family transcription factors. (researchsquare.com)
regulating the expression of target genes. (sagepub.com)
Assessment, transcription aspect binding web-site analysis In one more evaluation, we checked no matter whether the set our process to static information by removing the subscript referring on the time stage from Equation, of target genes containing regarded binding sites for any specific TF have been enriched among the youngster nodes of your JASPAR database. (cret-signal.com)

FZDs are receptors for WNT proteins. (guidetopharmacology.org)
Comments: FZD 6 -mediated G αo activation in an E. coli reconstitution system has been shown [ bona fide G Protein-Coupled Receptors.">9 ]. (guidetopharmacology.org)
These pericryptal myofibroblasts produce the Wnt signalling ligands which bind to Frizzled receptors as well as morphogenetic protein (BMP) antagonists gremlin 1(GREM1), gremlin 2 (GREM2) on basal epithelial stem cells and also can modulate notch signaling. (biomedcentral.com)
WNT signals are context-dependently transduced to both pathways based on the expression profile of WNT, SFRP, WIF, DKK, Frizzled receptors, coreceptors, and the activity of intracellular WNT signaling regulators. (aacrjournals.org)
The nulliparous hypermethylation of FZD1 suggests an up-regulation with the CCR8 Agonist Biological Activity Frizzled loved ones receptors and via this an up-regulation of all three forms of Wnt signaling, indeed, we observed a slight overexpression of this gene within the parous females (not statistically substantial). (scdinhibitor.com)
On the other hand, in spite of the Wnt signaling pathways getting seemingly up-regulated, crucial genes inside the pathways seem within our data to be down-regulated, thus altering the outcome of your signals sent by means of the Frizzled receptors. (scdinhibitor.com)
During vesiculation, bioactive lipids and receptors re- main associated with vesicle membranes, and cytosolic proteins and nucleic acids are contained within the vesicles [11]. (unicyte.ch)

Using ILC cell lines, we previously demonstrated that ER regulates a distinct gene expression program in ILC cells, and we hypothesized that these ER-driven pathways modulate the endocrine response in ILC. (nih.gov)
The Y-linked sex determining gene SRY regulates this process by initiating a pathway of gene and protein expression, including the expression of critical autosomal genes such as SOX9 . (plos.org)
Indian hedgehog (Ihh) and PTHrP signaling play crucial roles in regulating the onset of chondrocyte hypertrophy by forming a negative feedback loop, in which Ihh signaling regulates chondrocyte hypertrophy by controlling PTHrP expression. (silverchair.com)
During the development of the female reproductive system, the WNT4 protein regulates the formation of the Müllerian ducts. (medlineplus.gov)
In addition, the WNT4 protein regulates the production of androgens. (medlineplus.gov)
Rab7 regulates endothelial tight junction protein trafficking and paracellular permeability of the blood-brain barrier after ischemic stroke. (columbia.edu)

These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and embryogenesis. (wikipedia.org)
The ILC cell lines MDA-MB-134-VI, SUM44PE, and BCK4 were used to assess WNT4 gene expression and regulation, as well as the role of WNT4 in estrogen-regulated proliferation. (nih.gov)
We observed that ER regulation of WNT4 correlated with use of an ER binding site at the WNT4 locus, specifically in ILC cells. (nih.gov)
ILC-LTED cells remained dependent on WNT4 for proliferation, by either maintaining ER function and WNT4 regulation or uncoupling WNT4 from ER and upregulating WNT4 expression. (nih.gov)
and caused persistent down-regulation of basic-helix-loop-helix transcription factor expression ( Hes1, Hey1, Heyl ) in the vagina, leading to estrogen-independent epithelial cell proliferation. (diethylstilbestrol.co.uk)
In mice treated prenatally with DES there was a significant increase in enhancer of Zeste homolog 2 (EZH2) protein and EZH2 activity (measured by increased mammary histone H3 trimethylation)-a histone methyltransferase that may be linked to breast cancer risk and epigenetic regulation of tumorigenesis, as well as an increase in adult mammary gland EZH2. (diethylstilbestrol.co.uk)
Moreover, we describe experiments showing the presence of activated MKK4, a direct target of MAP3K4, and activated p38 in the coelomic region of the XY gonad at 11.5 d post coitum, establishing a link between MAPK signalling in proliferating gonadal somatic cells and regulation of Sry expression. (plos.org)
Loss of regulation by WNT4 likely disrupts development of the female reproductive system and induces abnormal production of androgens, leading to the features of Müllerian aplasia and hyperandrogenism. (medlineplus.gov)
structural genes encode proteins that are not involved in gene regulation. (cret-signal.com)
Although extensive knowledge has been obtained regarding biomarkers and the therapeutic targets that affect cancer cell properties, few studies have investigated the role of mutual regulation among these proteins in GC or their underlying mechanisms. (oncotarget.com)
Kinex antibody microarray analyses was used to investigate the regulation of 188 protein kinases, 24 protein phosphatases, and 170 other regulatory proteins during meiotic maturation of immature germinal vesicle (GV+) pig oocytes to maturing oocytes that had completed meiosis I (MI), and fully mature oocytes arrested at metaphase of meiosis II (MII). (appliedbioinfo.com)
The regulation of these proteins were also examined in parallel during the meiotic maturation of bovine, frog, and sea star oocytes with the Kinex antibody microarray. (appliedbioinfo.com)

In this review, we highlighted some of the main gene networks and regulatory mechanisms involved in the plasticity of sex-determining pathways that help to establish a functional ovary that can nurture the follicles to generate a good ovarian reserve, both in quantity and quality. (lidsen.com)
To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
These survival mechanisms include accumulation of organic osmolytes and increased expression of heat shock proteins through numerous pathways, resulting in osmotolerance. (biomedcentral.com)

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. (wikipedia.org)
The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)

Regardless of sex, though, WNT4 is needed for cell proliferation. (wikipedia.org)
In ILC and ILC-LTED cells, WNT4 led to suppression of CDKN1A/p21, which is critical for ILC cell proliferation. (nih.gov)
Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)

The canonical pathway will be the only 1 to involve beta-catenin, which can be the TCF/LEF binding protein responsible for improved transcription and EMT [57,58]. (scdinhibitor.com)
Sclerostin binds to LRP5 and LRP6 and inhibits canonical Wnt signaling by obstructing the binding of Wnt proteins towards the extracellular parts of LRP5 and LRP6. (tech-strategy.org)

The endoplasmic reticulum (ER) is the major subcellular compartment that is involved in protein folding and secretion. (elifesciences.org)
Hyperosmotic/-tonic media upregulated Lcn2-R by ~4-fold and decreased Lcn2 expression/secretion, along with Wnt/β-catenin activation, in IMCD cells. (biomedcentral.com)
In addition to their indirect effects on osteoclastogenesis that are mediated by controlling OPG expression and secretion by osteoblasts/osteocytes, Wnts act directly on osteoclasts. (tech-strategy.org)

The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
In multicellular organisms, signal transduction is essential to these processes, yet while our understanding of how signals regulate gene expression is relatively advanced, our understanding of how signals direct the acquisition of specific shapes and forms is less advanced. (stanford.edu)
Antibody Microarray Analyses of Signal Transduction Protein Expression and Phosphorylation during Porcine Oocyte Maturation. (appliedbioinfo.com)

Lastly, MuSK is the receptor for WNT4, activated through tyrosine phosphorylation. (wikipedia.org)
We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)
Anatomical profiling of G protein-coupled receptor expression. (guidetopharmacology.org)

To date, several of the Wnt proteins have been shown to play a role in skeletal development and homeostasis aswell as joint development in human beings and mice, including Wnt1, Wnt3a, Wnt4, Wnt5, Wnt5a, Wnt7a, Wnt10b, and Wnt14. (tech-strategy.org)

If both are deficient in XY mice, the outcome is less expression of SRY and downstream targets. (wikipedia.org)
If this signaling is deficient in XY mice, female genes are unrepressed. (wikipedia.org)
Moreover, we showed enhanced expression of GM3 via activation of the GM3 synthase gene upon administration of valproic acid (VPA) is effective in suppressing albuminuria and podocyte injury in mice with anti-nephrin antibody-induced podocytopathy. (bvsalud.org)
Here, we investigated the therapeutic effect of enhanced GM3 expression via VPA on podocyte injury using streptozotocin-induced diabetic nephropathy model mice. (bvsalud.org)
Prenatal exposure to DES caused hypermethylation of the Hoxa10 gene in the uterus of mice and was linked to uterine hyperplasia and neoplasia later in life. (diethylstilbestrol.co.uk)
DES also altered the expression of genes commonly involved in metabolism or endometrial cancer in mice, and it activated nongenomic signaling in uterine myometrial cells and increased the incidence of cystic glands in rats. (diethylstilbestrol.co.uk)
These data demonstrate that MAP3K4-dependent signalling events are required for normal expression of Sry during testis development, and create a novel entry point into the molecular and cellular mechanisms underlying sex determination in mice and disorders of sexual development in humans. (plos.org)
To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)

a protein component of a ubiquitin ligase complex) and hypoxia-inducible factors (a family of transcription factors activated by hypoxia). (lww.com)
In cells receive the WNT signal, β-catenin is stabilized and joins the addition, AXIN2 has also been independently associated with DNA-bound T-cell factor family of transcription proteins for tooth agenesis and non-syndromic cleft lip palate (NSCLP). (sagepub.com)
Below is a small sample of the many proteins, growth factors, transcription factors it contains. (plurisomes.com)

WNT4 is involved in a couple features of pregnancy as a downstream target of BMP2. (wikipedia.org)
ILC and ILC-LTED cell lines were used to identify upstream regulators and downstream signaling effectors of WNT4 signaling. (nih.gov)
Among the main element downstream targets of the systems are NF-B, mitogen-activated proteins kinases, and people from the IRF family members (5). (cancer-ecosystem.com)
Neonatal exposure to DES caused persistent changes in expression of IGF-1 and its downstream signaling factors in mouse vaginas. (diethylstilbestrol.co.uk)
In the developing male (XY) gonad, sex-determining region of the Y (SRY) protein acts to up-regulate expression of the related gene, SOX9 , a transcriptional regulator that in turn initiates a downstream pathway of testis development, whilst also suppressing ovary development. (plos.org)
Our data indicate, for the first time, a requirement for MAPK signaling in the developing XY gonad in order to facilitate normal expression of SRY and the downstream testis-determining genes and also suggest that reduced dosage of MAP3K4 may be the cause of a previously described autosomal sex-reversing mutation in the mouse. (plos.org)
Yes-associated protein 1 (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) function as the key downstream effectors of the Hippo pathway [ 10 ]. (oncotarget.com)

Several mutations are known to cause loss of function in WNT4. (wikipedia.org)
nonpolyposis CRC syndrome (Lynch syndrome), involve mutations Somatic mutations in the AXIN-complex proteins associated with in germline or in repair genes. (sagepub.com)
Mutations in the WNT4 gene cause Müllerian aplasia and hyperandrogenism. (medlineplus.gov)
Lately, missense mutations in the gene had been identified in a kind of autosomal dominating early-onset osteoporosis and a serious type of osteogenesis imperfecta [26]. (tech-strategy.org)

Increases in apparent protein levels of protein kinases accounted for most of the detected changes during the GV to MI transition, whereas reduced protein kinase levels and increased protein phosphorylation characterized the MI to MII transition. (appliedbioinfo.com)
Western blotting analyses confirmed altered expression levels of Bub1A, IRAK4, MST2, PP4C, and Rsk2, and the phosphorylation site changes in the kinases Erk5 (T218 + Y220), FAK (S722), GSK3-beta (Y216), MEK1 (S217 + S221) and PKR1 (T451), and nucleophosmin/B23 (S4) during pig oocyte maturation. (appliedbioinfo.com)

By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
Furthermore, we found that these hypoxic exosomes contained Wnt4 depending on HIF1α. (ijbs.com)
Taken together, the results of our study indicate that hypoxia may stimulate tumour cells to release Wnt4-rich exosomes that are delivered to normoxic cells to enhance prometastatic behaviours, which might provide new targets for CRC treatment. (ijbs.com)
As previously reported, colorectal cancer cell-derived exosomes have important roles in tumour progression including invasion, angiogenesis, immune modulation and distal metastasis through effectively delivering microRNAs, mRNAs and proteins ( 10 - 12 ). (ijbs.com)
Consistent with these findings, we found that Wnt4 was enriched in exosomes released from hypoxic CRC cells and mediated the functions of endothelial cells ( 13 ). (ijbs.com)

WNT4 deficiency--a clinical phenotype distinct from the classic Mayer-Rokitansky-Kuster-Hauser syndrome: a case report. (medlineplus.gov)

For instance, TAFIIs 105 is now encoded, a subunit of the TATA binding protein for RNA polymerase in ovarian follicle cells. (wikipedia.org)
In 2013, Dylan began working at QIMR Berghofer and has undertaken the functional follow-up of large-scale genetic studies of breast, endometrial and ovarian cancer to identify the likely causal variants and genes that mediate associations with cancer risk and survival. (edu.au)

This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
Exosomal Wnt4 mediated hypoxic exosome-mediated migration and invasion of normoxic CRC cells. (ijbs.com)
Moreover, exosomal Wnt4 enhanced β-catenin translocation to the nucleus in normoxic CRC cells. (ijbs.com)

It also up-regulated Wnt4 , a factor correlated with the stratification of epithelial cells , in mouse vaginas. (diethylstilbestrol.co.uk)

Surprisingly, there was an increase in Bmp15, but not Gdf9, Figla, Nobox transcripts or somatic-specific transcripts Amh and Wnt4 in cultured Lats1 mutant ovaries. (appliedbioinfo.com)

In addition, we found that bone morphogenetic protein (Bmp) and Wnt/β-catenin signaling in the cartilage may both mediate the effect of upregulated Ihh signaling in promoting chondrocyte hypertrophy. (silverchair.com)

Human ortholog(s) of this gene implicated in Mullerian aplasia and hyperandrogenism. (zfin.org)

Hence, molecules Dental agenesis is one of the most common congenital anomalies such as WNT (WNT4, WNT6, and WNT10) and AXIN2 play an in human dentition. (sagepub.com)
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. (appliedbioinfo.com)

Wnt4 promotes the proliferation of cancer stem cells in response to progesterone in breast cancer ( 20 ). (ijbs.com)

Afterwards, he continued working at the University of Otago as a Research Fellow, studying the biological function of genes involved with inflammatory bowel disease. (edu.au)

The absence of WNT4 is required for male sex development. (wikipedia.org)
Lastly, the absence of WNT4 also affects the expression of other genes that function in lung development such as Sox9 and FGF9. (wikipedia.org)
Sex determination in mammals is controlled by the presence or absence of the Y-linked gene SRY . (plos.org)
In the absence of AXIN2 SNP rs2240308 has been mapped at human chromosome WNT, β-catenin protein is degraded by the proteasome via action 17q23-q24. (sagepub.com)

Consistently, in vitro analysis confirmed the same expression pattern of WNT7b. (researchsquare.com)
We found that upregulating Ihh signaling in the developing cartilage by treating PTHrP -/- limb explants with sonic hedgehog (Shh) protein in vitro, or overexpressing Ihh in the cartilage of PTHrP -/- embryos or inactivating patched 1( Ptch1 ), a negative regulator of hedgehog (Hh) signaling, accelerated chondrocyte hypertrophy in the PTHrP -/- embryos. (silverchair.com)

Second, networks of genes and hormones regulate the advancement of sex determination and gonad differentiation, known as the gonadal sex phase. (lidsen.com)
Wnt4 action in gonadal development and sex determination. (medlineplus.gov)

This protein is also involved in development of the ovaries, from before birth through adulthood, and is important for development and maintenance of egg cells (oocytes) in the ovaries. (medlineplus.gov)
Here, the expression of hippo pathway components were including Wwtr1, Stk4, Stk3, Lats2 and Yap1 transcripts were decreased by 50% in mouse ovaries between 2 and 8 days of age while expression was maintained from 8 days to 21 days and after priming with eCG. (appliedbioinfo.com)

CDKN1A knockdown partially reversed the effects of WNT4 knockdown. (nih.gov)
During the MI to MII period, many of the MI-associated increased levels of the proteins and phosphosites were completely or partially reversed. (appliedbioinfo.com)

The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)

This condition is inherited in an autosomal dominant pattern, which means one copy of the altered gene in each cell is sufficient to cause the disorder. (medlineplus.gov)

WNT4 probably functions by activating BMP4, a known smooth muscle differentiation factor. (wikipedia.org)

A WNT4 mutation associated with Mullerian-duct regression and virilization in a 46,XX woman. (medlineplus.gov)

González, F. 2019-10-16 00:00:00 BDJOpen www.nature.com/bdjopen ARTICLE OPEN Variations in AXIN2 predict risk and prognosis of colorectal cancer 1 2 3 4 3 5 L. Otero , E. Lacunza , V. Vasquez , V. Arbelaez , F. Cardier and F. González OBJECTIVE: Colorectal cancer (CRC) and hypodontia are frequent and different diseases with common genes are involved in their etiology. (sagepub.com)

FGF signaling suppresses WNT4, acting in a feed forward loop triggered by SOX9. (wikipedia.org)
Expression analysis of mutant XY gonads at the same stage also reveals a dramatic reduction in Sox9 and, crucially, Sry at the transcript and protein levels. (plos.org)

WNT, fibroblast growth factor (FGF), Notch, Hedgehog, and transforming growth factor β/bone morphogenetic protein signaling network are implicated in the maintenance of tissue homeostasis by regulating self-renewal of normal stem cells as well as proliferation or differentiation of progenitor (transit-amplifying) cells. (aacrjournals.org)

WNT4 is a secreted protein that in humans is encoded by the WNT4 gene, found on chromosome 1. (wikipedia.org)
In humans, WNT4 also suppresses 5-α reductase activity, which converts testosterone into dihydrotestosterone. (wikipedia.org)

We then checked for enrichment of these genes among the inferred child nodes of the corresponding TFs in every single network with Fishers actual check. (cret-signal.com)

Proper rules from the gene items comprising these systems by transcriptional and post-transcriptional digesting isn't just very important to selective pathogen eradication also for avoiding excessive build up of cytokines such as for example interferon-, interferon-, IL-6, and TNF- that start the host protection against microbial assault (6). (cancer-ecosystem.com)

Analysis of embryonic XY gonads suggests that sex reversal is caused by delayed and reduced expression of the sex-determining gene SRY . (plos.org)

DES caused histone deacetylation in the promoter region of the cytochrome P450 side chain cleavage (P450scc) gene . (diethylstilbestrol.co.uk)
Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. (appliedbioinfo.com)

Gene knockouts result in hair and bristle patterning defect. (guidetopharmacology.org)

In this study, WNT7b expression was measured by immunohistochemical staining of 100 cases of surgically resected human colorectal cancerous tissues as well as matched adjacent normal tissues constructed as tissue microarrays. (researchsquare.com)
Immunohistochemical analysis demonstrated that in the fluoxetine-exposed group HoxB5 is more expressed in the mesenchymal cells, but in the control group the expression is limited to alveolar cells. (ac.ir)

The human WNT gene family consists of 19 members, encoding evolutionarily conserved glycoproteins with 22 or 24 Cys residues ( 1 ). (aacrjournals.org)

Anatomy26

Organisms17

Diseases5

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes91

Disciplines and Occupations3

Information Science7

Health Care2

Signaling pathway4

Wingless-type MMTV integration site family5

Ligand3

Target genes4

Receptors7

Regulates6

Regulation12

Pathways3

Encode2

Cell proliferation3

Canonical2

Secretion3

Signal Transduction3

Receptor3

Wnt51

Mice8

Transcription3

Downstream7

Mutations4

Kinases2

Exosomes5

Phenotype1

Ovarian2

Exosomal3

Epithelial1

Somatic1

Mediate1

Aplasia and hyperandrogenism1

Molecules2

Promotes1

Biological1

Absence4

Vitro2

Gonadal2

Ovaries2

Partially reversed2

Germline1

Autosomal1

BMP41

Mullerian1

Diseases1

Sox92

Differentiation1

Humans2

Enrichment1

Transcriptional1

Gonads1

Cleavage2

Defect1

Immunohistochemical2

Family consists1