Gene expression hybrid cells. Medical search. Frequent questions

The two expression profile datasets were integrated and we found out coexpression of differentially expressed genes (DEGs) using bioinformatics methods. (hindawi.com)
With the development of the gene expression profile, comparisons of differentially expressed genes that participate in the regulation of pathophysiological conditions in pathological tissue and normal tissue were available and convenient, and mRNAs gene expression profiles have been carried out in many diseases including TAD [ 6 ]. (hindawi.com)
Then we downloaded another microarray dataset GSE52093 [ 9 ] and screened out the coexpression of differentially expressed genes (DEGs) between TAD patients and non-TAD patients. (hindawi.com)
Out of 324 differentially expressed genes, 184 were up-regulated and 140 were down-regulated. (omicsdi.org)
For young rats, the stress response pathway was highly regulated (20 differentially expressed genes at each time point) and was accompanied by robust DNA demethylation, tissue remodeling, and isometric torque recovery. (cdc.gov)
For old rats, a muted transcriptional profile (13 and 2 differentially expressed genes at 6 and 120 h, respectively) coincided with deficiencies in demethylation, muscle remodeling, and torque recovery. (cdc.gov)

He and Martin Rodbell shared the 1994 Nobel Prize in Physiology or Medicine "for their discovery of G-proteins and the role of these proteins in signal transduction in cells. (wikipedia.org)
G proteins are a vital intermediary between the extracellular activation of receptors (G protein-coupled receptors) on the cell membrane and actions within the cell. (wikipedia.org)
It was Gilman who actually discovered the proteins that interacted with the GTP to initiate signalling cascades within the cell, and thus, giving the name G proteins. (wikipedia.org)
In this system when two proteins interact with each other, they activate the expression of a reporter gene that produces a colored product within the yeast cell. (genome.gov)
The yeast two-hybrid system was developed to allow researchers to detect proteins that physically interact with each other in the cell. (genome.gov)
These proteins get their name because they bind to genes and turn on transcription. (genome.gov)
The idea behind the yeast two-hybrid system is to separate the two domains of the transcription factor, attach each domain to different proteins being studied, and give them an opportunity to interact within the cell. (genome.gov)
Typically when using the yeast two-hybrid system, one bait protein is used and it is tested against a library of many different prey proteins. (genome.gov)
Expression of the reporter gene indicates that the bait and prey proteins interact within the cell. (genome.gov)
We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
This gene encodes a member of the LYR family of proteins that contain a highly conserved tripeptide (LYR) motif near the N-terminus. (nih.gov)
Gene sequencing revealed that this group was in fact the same FGF2 protein and that it was a member of a family of FGF proteins. (wikidoc.org)
It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins . (wikidoc.org)
GRFs are a large gene family with highly conserved proteins. (biomedcentral.com)
That's because EF-Tu is a hub protein, interacting with 50 or more other proteins as it carries out its function in the cell. (uncommondescent.com)
Photoreceptor proteins are proteins present on the cell membrane of optic nerve cells and absorb light. (justia.com)
Opsins are membrane proteins present on the cell membrane of optic nerves. (justia.com)
The WD-repeat protein family consists of a large group of structurally related yet functionally diverse proteins found predominantly in eukaryotic cells. (shengsci.com)
Finally, these data provide only a basis for understanding this complex network of functionally relevant genes and proteins related to the adaptive/maladaptive process, which is currently limited. (cdc.gov)
The most critical of these proteins is the signal transducer and activator of transcription 5b (STAT5b), which couples GH binding to the activation of gene expression that leads to the intracellular effects of GH, including synthesis of IGF-I, insulin-like growth factor binding protein 3 (IGFBP3), and ALS. (medscape.com)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
Two separate genes (RhCE and RhD), located on the short arm of chromosome 1, encode Rh proteins. (medscape.com)
Production of 2 distinct proteins from the RHCE gene is due to alternative splicing of messenger RNA. (medscape.com)
Although, the number of omic techniques is ever expanding, the most developed omics technologies are high throughput DNA sequencing, transcriptomics (focused on gene expression), epigenomics (focused on epigenetic regulation of gene expression), proteomics (focused on large sets of proteins, the proteome) and metabolomics (focused on large sets of metabolites, the metabolome). (who.int)
Oxidant damage to cellular DNA, proteins (including the epigenome), and lipids can occur when reactive oxygen species escape cell antioxidant and repair mechanisms. (who.int)

Our results resolve the outstanding question of how hybrids in native genomic contexts interact with replication to cause mutagenesis and shape genome organization. (biorxiv.org)
My lab studies how the genomic sequences that control gene expression function and evolve. (berkeley.edu)
For each of the approximately 40 transcription factors critical in shaping anterior-posterior and dorsal-ventral patterns, our goals are to: 1) measure the factor s in vitro affinity to each of its potential target sequences, 2) identify the genomic regions bound by each factor in living embryos, 3) determine the expression pattern of the factor and its targets in three-dimensions at cellular resolution. (berkeley.edu)
Chromosome localization and genomic structure of the KiSS-1 metastasis suppressor gene (KISS1). (shengsci.com)
100 predicted genomic off-target loci in 293T and 293FT cells. (cdc.gov)

Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping. (nih.gov)

RNA:DNA hybrids such as R-loops affect genome integrity and DNA replication fork progression. (biorxiv.org)
The overall impacts of naturally occurring RNA:DNA hybrids on genome integrity, and the relative contributions of ribonucleases H to mitigating the negative effects of hybrids, remain unknown. (biorxiv.org)
Here, we investigate the contributions of RNases HII (RnhB) and HIII (RnhC) to hybrid removal, DNA replication, and mutagenesis genome-wide. (biorxiv.org)
Evo2 can save genealogical information for each genome so users may review the progression of the genetic algorithm to see how certain genes have evolved over time. (modulusfe.com)
Scientists armed with whole genome sequence data can now ask questions about genome structure and organization that impact gene expression. (genengnews.com)
We are a hybrid computational and experimental lab who couple genome-scale computational and experimental analysis of gene regulation in Drosophila melanogaster and Saccharomyces cerevisiae with extensive analysis of comparative sequence data and experimental analysis of species closely related to these model systems. (berkeley.edu)
We focus on short evolutionary timescales where it is possible to couple specific changes in genome sequences with alterations in gene regulation and expression. (berkeley.edu)
The detailed experimental data we are generating for D. melanogaster , and the genome sequences of 12 Drosophila species are a tremendous resource for studying the evolution of gene regulation. (berkeley.edu)
Both crRNA-tracrRNA duplexes and sgRNAs can be used to target SpCas9 for multiplexed genome editing in eukaryotic cells 1 , 3 . (cdc.gov)

Mules are infertile because they inherit 32 chromosomes from their horse parent, but only 31 chromosomes from their donkey parent-and so have an odd chromosome that they cannot pair-off when they make sperm or egg cells. (elifesciences.org)
However, even if a hybrid inherits the same number of chromosomes from each parent, if the chromosomes from the two parents have different structures, the hybrid may still be infertile. (elifesciences.org)
Although hybrids of these two species inherit three chromosomes from each parent, the majority of spores (the yeast equivalent of sperm) that these hybrids produce fail to develop into new yeast cells. (elifesciences.org)
Evo2 simulates every natural process from mate selection to DNA packaging and complete meiosis (cell division that reduces chromosomes by half). (modulusfe.com)
In this study, 20 GRF gene members of ginseng were systematically identified and found to be distributed on 13 chromosomes. (biomedcentral.com)
The ginseng GIF gene family has only ten members, which are distributed on ten chromosomes. (biomedcentral.com)
The Rh-negative phenotype represents absence of D protein on RBCs and most commonly results from deletion of the RHD gene on both chromosomes. (medscape.com)

The aim of the present study was to investigate the association between tumor protein 53 (TP53) gene deletion and protein expression and clinical features in esophageal squamous cell carcinoma (ESCC), and to evaluate the predictive value of these two characteristics in the prognosis of ESCC. (spandidos-publications.com)
The positive rate of p53 protein expression was 54.5% (201/369) in the multi‑ethnic group, and was significantly different between sex (P=0.026) and between tumor differentiation groups (P=0.032). (spandidos-publications.com)
Tumor protein 53 ( TP53 ) is a recognized tumor suppressor gene located on chromosome 17q13.1. (spandidos-publications.com)
Mutations in TP53 can lead to the loss of these functions, inducing uncontrolled cell growth and the promotion of tumor development ( 11 , 12 ). (spandidos-publications.com)
We have investigated the sensitivity of the cisplatin-resistant enterohepatic tumor cell lines LS174T/R (human colon adenocarcinoma), WIF-B9/R (rat hepatoma-human fibroblast hybrid), and Hepa 1-6/R (mouse hepatoma) to free and liposome-encapsulated cytostatic bile acid derivatives Bamet-R2 and bamet-UD2. (aspetjournals.org)
Using a lentiviral vector containing either H-Ras oncogene and a hairpin targeting p53 or both tumor suppressor genes, NF-1 and p53, Dr. Friedmann-Morvinski can induce the formation of a glioma tumor in mice injected in the brain. (genengnews.com)
The induction of the dedifferentiation occurs only when two hits occur in the same cell: either H-Ras overexpression and loss of p53 or loss of tumor suppressors NF1 and p53. (genengnews.com)
Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)
Compared with conventional cancer treatments, the main advantage of oncolytic virotherapy is its tumor-selective replication followed by the destruction of malignant cells without damaging healthy cells. (biomedcentral.com)
Oncolytic viruses (OVs) specifically induce cancer cells death by infection of and spreading on tumor cells through different mechanisms such as direct lysis effect or adjacent cells fusion. (biomedcentral.com)
The ability of OVs to selectively infect tumor cells is mainly associated with the disruption of type-I interferon pathway, which represents the first line of defense against viral infections. (biomedcentral.com)
In this case, the stimulation of endoplasmic reticulum (ER) stress and subsequent immunogenic cell death (ICD) process in OVs-infected cells elicit a range of anti-tumor immune responses. (biomedcentral.com)
On the other hand, the proliferation of oncolytic viruses into target cells and subsequent recognition of viral elements by pattern recognition receptors (PRRs) such as toll-like receptors can positively affect the development of the innate and adaptive immune responses against tumor cells (Fig. 1 ) [ 6 ]. (biomedcentral.com)
Schematic illustration of the multimodality role of virotheranostics in treatment and tracking of tumor cells. (biomedcentral.com)

B-cell lymphomas arise from mutations affecting B-cell differentiation, particularly within germinal centers (GCs) where B-cells interact with T follicular helper cells ( 1 ). (iiarjournals.org)
It has an essential role in B-cell development, influencing the transition of memory B-cells to plasma cells, while mutations in the TBL1XR1 gene have been observed to inhibit this progression and promote lymphomagenesis ( 7 , 8 ). (iiarjournals.org)
However, the RHD gene has significant heterogeneity, and several inherited mutations and rearrangements in its structure can result in a lack of expressions of the RhD phenotype as well. (medscape.com)
Important examples of such mutations include the RHD pseudogene and RHD-CE-D hybrid gene. (medscape.com)

For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
Human sodium/iodide symporter (hNIS) is considered as one of the most prevalent nuclear imaging reporter transgenes that provides precise information regarding the kinetics of gene expression, viral biodistribution, toxicity, and therapeutic outcomes using the accumulation of radiotracers at the site of transgene expression. (biomedcentral.com)

While imprinting perturbations are widely associated with developmental abnormalities, the intricate regional interplay between imprinted genes makes interpreting the contribution of gene dosage effects to phenotypes a challenging task. (nature.com)
Yet, the intricate form of epigenetic control over the parent-specific expression of multiple genes in an imprinted cluster poses difficulties when trying to decipher the relative contribution of changes in imprinted gene dosage to the resulting physiological phenotypes. (nature.com)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

The δ subunit mRNA was present at wild-type levels in the mutant granule cells, indicating a post-translational loss of the δ subunit. (jneurosci.org)
In the present study, we use mRNA microarray to acquire differential expression profiles in human TAD tissues and non-TAD tissues. (hindawi.com)
Furthermore, elevated levels of R-loops and of mRNA for paraspeckle genes in patient cells are linearly correlated with Multiple Myeloma progression. (uni-koeln.de)
Himasthla elongata: effect of infection on expression of the LUSTR-like receptor mRNA in common periwinkle haemocytes. (evrogen.ru)
Cytoplasmic expression systems triggered by mRNA yield increased gene expression in post-mitotic neurons. (ox.ac.uk)
We have developed and evaluated a hybrid mRNA/DNA system designed to bypass the nuclear barrier to transfection and facilitate cytoplasmic gene expression. (ox.ac.uk)
This system, based on co-delivery of mRNA(A64) encoding for T7 RNA polymerase (T7 RNAP) with a T7-driven plasmid, produced between 10- and 2200-fold higher gene expression in primary dorsal root ganglion neuronal (DRGN) cultures isolated from Sprague-Dawley rats compared to a cytomegalovirus (CMV)-driven plasmid, and 30-fold greater expression than the enhanced T7-based autogene plasmid pR011. (ox.ac.uk)
Cell-free assays and in vitro transfections highlighted the versatility of this system with small quantities of T7 RNAP mRNA required to mediate expression at levels that were significantly greater than with the T7-driven plasmid alone or supplemented with T7 RNAP protein. (ox.ac.uk)
We have also characterized a number of parameters, such as mRNA structure, intracellular stability and persistence of each nucleic acid component that represent important factors in determining the transfection efficiency of this hybrid expression system. (ox.ac.uk)
The results from this study demonstrate that co-delivery of mRNA is a promising strategy to yield increased expression with plasmid DNA, and represents an important step towards improving the capability of non-viral vectors to mediate efficient gene transfer in cell types, such as in DRGN, where the nuclear membrane is a significant barrier to transfection. (ox.ac.uk)

The protein transducin β-like 1 X-linked receptor 1 (TBL1XR1) regulates gene expression as part of the nuclear receptor corepressor (NCoR)/silencing mediator for retinoid and thyroid receptors (SMRT) corepressor complex ( 4 ). (iiarjournals.org)

Here, removal of intact α 6 protein from cerebellar granule cells allowed perturbations in other subunit levels to be studied. (jneurosci.org)
Exon 8 of the mouse α 6 subunit gene was disrupted by homologous recombination. (jneurosci.org)
In α 6 −/− granule cells, the δ subunit was selectively degraded as seen by immunoprecipitation, immunocytochemistry, and immunoblot analysis with δ subunit-specific antibodies. (jneurosci.org)
Because in α 6 −/− neurons the remaining α 1 , β 2/3 , and γ 2 subunits cannot rescue the δ subunit, certain potential subunit combinations may not be found in wild-type cells. (jneurosci.org)
For example, cerebellar granule cells express six subunit genes abundantly (α 1 , α 6 , β 2 , β 3 , γ 2 , and δ), and so they probably have several distinct GABA A receptor subtypes of unknown subunit stoichiometry. (jneurosci.org)
The FaeG subunit is essential for the binding of the three F4 variants to host cells. (k-state.edu)
Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. (k-state.edu)
IGF-I deficiency can be the result of GH resistance or insensitivity due to genetic disorders of the GH receptor causing GH receptor deficiency (growth hormone receptor deficiency [GHRD], Laron syndrome) or postreceptor defects, including the principal transduction agent STAT5b, the IGF-I/IGFBP3 stabilizer acid labile subunit (ALS), the IGF-I gene, or the IGF-I receptor. (medscape.com)

At least three distinct meiotic drive alleles, one on each S. kambucha chromosome, independently contribute to hybrid infertility by causing nonrandom spore death. (elifesciences.org)
Mammalian parental imprinting is a form of epigenetic regulation that causes genes to be expressed from only one chromosome homolog according to parent-of-origin 1 , 2 . (nature.com)

Approximately one-half of new cases each year are recorded in China, and the majority is squamous cell carcinoma ( 3 - 5 ). (spandidos-publications.com)
HNSCC is the sixth oropharyngeal squamous cell carcinoma (OSCC). (cdc.gov)
the goal is to cause as little functional squamous cell carcinoma type (HNSCC) and includes and cosmetic damage as possible ( 6 , 9 ). (cdc.gov)
A growing body of research shows that human papillomavirus (HPV) is a common and increasing cause of oropharyngeal squamous cell carcinoma (OSCC). (cdc.gov)
We focus on the role of HPV in the increased incidence of oropharyngeal squamous cell carcinoma (OSCC), the head and neck cancer in which HPV is most commonly found ( 4 ). (cdc.gov)
Head and neck cancer most commonly is of the squamous cell carcinoma type (HNSCC) and includes cancers of the oral cavity, oropharynx, hypopharynx, larynx, sinonasal tract, and nasopharynx. (cdc.gov)

These data demonstrate that African green monkey-like natural killer cell differentiation profiles can be rescued in rhesus macaques to promote viral clearance in tissues. (nature.com)
define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)
Background/Aim: B-cell lymphomas are characterized by diverse genetic anomalies affecting B-cell differentiation. (iiarjournals.org)
The complexities in B-cell lymphoma, including the common diffuse large B-cell lymphoma (DLBCL) subtype, originate from accumulated genetic anomalies during this differentiation process ( 3 ). (iiarjournals.org)
[10] FGF2, in conjunction with BMP4 , promote differentiation of stem cells to mesodermal lineages. (wikidoc.org)
After differentiation, BMP4 and FGF2 treated cells generally produce higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells. (wikidoc.org)
The proliferation and differentiation of hBMSC, the markers of bone formation (ALP, COL-1) and angiogenesis (VEGF, HIF-1α), and wnt/ß-catenin related-signaling pathway gene were studied by cell culture. (bvsalud.org)
Transferable cell-secreted extracellular matrices enhance osteogenic differentiation. (ca.gov)
We then investigated its capacity to accelerate naïve hMSC osteogenic differentiation by quantifying gene expression, intracellular alkaline phosphatase production, and calcium deposition when cultured on DMs or tDMs. (ca.gov)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

Wild-type TP53 can inhibit the cell cycle and activate apoptosis-related genes that induce apoptosis and regulate cell proliferation ( 10 ). (spandidos-publications.com)
Amy Radunz, a nutritionist from the department of animal sciences at the University of Wisconsin in River Falls, has partnered with molecular biologist Hasan Khatib, an associate professor at University of Wisconsin-Madison, to ask how differences in maternal diet during pregnancy induce gene expression and DNA methylation changes in fetal tissues in sheep. (genengnews.com)
The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)

Here, we identify and characterize the single Drosophila ortholog of the HB9/MNR2 gene family. (jneurosci.org)
Thus, Drosophila HB9 is required in a subset of motoneurons and interneurons for establishing proper axon projections but does not have a general role in distinguishing motoneuron and interneuron cell types. (jneurosci.org)
Drosophila genes expressed specifically in all motoneurons have not been described, although a growing number of genes are known to be expressed in subsets of motoneurons. (jneurosci.org)
My lab is applying the high-resolution fluorescent imaging methods developed for D. melanogaster to systematically analyze gene expression, and dissect regulatory networks, in other Drosophila species and in several inbred lines of D. melanogaster . (berkeley.edu)
To take advantage of sequence diversity outside of the genus Drosophila, we are sequencing developmentally important loci from several non-Drosophilid fly families to provide insights into the underlying principles of gene regulation. (berkeley.edu)

Across all cells, hybrids accumulate most strongly in non-coding RNAs and 5′-UTRs of coding sequences. (biorxiv.org)
For Δ rnhB , hybrids accumulate preferentially in untranslated regions and early in coding sequences. (biorxiv.org)
Improved access to bioinformatics tools enables scientists to process, manage, and store raw data, annotate sequences to define genes, and quantitate RNA sequence data to get insight into gene expression patterns. (genengnews.com)
The locus control region (LCR) of mammalian beta-globin genes covers at least 17 kb at the 5' end of the gene cluster and has been implicated in chromatin domain opening, enhancement, and insulation from neighboring sequences. (shengsci.com)
We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
AtGIF1 , the first member of the GIF family, has been used as bait in yeast two-hybrid assays [ 14 ]. (biomedcentral.com)
Global transcriptome assays of both types of cells with or without 5-azacytidine treatment identified 640 genes, whose expression might be methylation-dependently down-regulated in the MG cells. (oncotarget.com)

In SIVagm infection, systemic IL-15 was associated with NK cell proliferation in lymph node (LN), while systemic IFNα correlated with NK cell cytotoxicity in LN 17 . (nature.com)
The associated immunodeficiency often involves impaired natural killer cell activity, absent delayed-type hypersensitivity, and a poor cell proliferation response to antigenic challenge. (medscape.com)

Deletion of either rnhB or rnhC triggers RNA:DNA hybrid accumulation, but with distinct patterns of mutagenesis and hybrid accumulation. (biorxiv.org)
Similarly, TP53 mutation does not always lead to the accumulation of p53 protein in cells. (spandidos-publications.com)
In resistant cells, overexpression of MRP1 and MRP2 was accompanied by reduced accumulation of cisplatin. (aspetjournals.org)
We systematically show that depletion of Che-1 produces a marked accumulation of RNA:DNA hybrids. (uni-koeln.de)

We will combine our mRNAs expression profile (6 TAD tissues versus 6 non-TAD tissues) and GSE52093 downloaded from the Gene Expression Omnibus (GEO) database. (hindawi.com)
The two mRNAs expression profiles contained 13 TAD aortic tissues and 11 non-TAD tissues. (hindawi.com)
The study also looked at the impact of paternally expressed genes: IGF2, PEG1, PEG3, DLK1, and DIO3 for transcriptomic and epigenomic alterations of the imprinted genes in fetal tissues. (genengnews.com)
Based on the available public RNA-Seq data, the expression patterns of PgGRF and PgGIF genes were analysed from 14 different tissues. (biomedcentral.com)
GRF genes are usually expressed at higher levels in young tissues but at lower levels in mature tissues [ 6 ]. (biomedcentral.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Expression is limited to RBCs, with an increasing density during their maturation, unlike the ABH system, which exists in a wide variety of tissues. (medscape.com)

Using mouse models with distinct deletions in an intergenic region controlling imprinting across the Dlk1-Dio3 domain, we link changes in genetic and epigenetic states to allelic-expression and phenotypic outcome in vivo. (nature.com)
One of the largest imprinted clusters in mammals is a 1.2 Mb domain encompassing the Dlk1 and Dio3 genes. (nature.com)

Upon antigen exposure, B-cells in the GC undergo somatic hypermutation, promoting the evolution of high-affinity antigen receptors and differentiating into memory B-cells or plasma cells ( 2 ). (iiarjournals.org)
These results described the changes of CH on the CB gene expression profile, affecting a possible interaction in between NOS and ET-1 receptors, as part of the adaptive CB response to CH. (omicsdi.org)
In this manner, the reduced detection of self MHC-I molecules by NK cell-inhibitory receptors coupled with an increase in activating signals by recognition of stress-induced surface ligands promote the cytolytic activity of NK cells. (biomedcentral.com)
In addition to these receptors, hybrid receptors consisting of a dimer from the IGF-I receptor paired with the insulin receptor, are ubiquitous and the respective expression of these receptors varies from tissue to tissue. (medscape.com)

B-cell receptor diversification, essential for adaptive immunity, depends on processes like V(D)J recombination and somatic hypermutation ( 1 ). (iiarjournals.org)
Structural organization of the human prostaglandin EP3 receptor subtype gene (PTGER3). (shengsci.com)
The GH molecule binds to its specific cell surface receptor (GHR), which dimerizes with another GHR molecule so that the single GH molecule is enveloped by 2 GHR molecules. (medscape.com)

The WRC domain can be combined with the cis-acting regions of downstream genes to regulate their expression. (biomedcentral.com)
For the DEGs at 2 dpi, Ingenuity Pathway Analysis predicted inhibition of multiple signaling pathways in response to NDV that regulate immune cell development and activity, neurogenesis, and angiogenesis. (escholarship.org)

This study aims at integrating two gene expression profile datasets and verifying hub genes and pathways involved in TAD as well as exploring potential molecular mechanisms. (hindawi.com)
With the emergence of hundreds of single-cell RNA-sequencing (scRNA-seq) datasets, the number of computational tools to analyze aspects of the generated data has grown rapidly. (biomedcentral.com)

0.05), and these candidate genes could be used as potential diagnostic biomarkers and therapeutic targets of TAD. (hindawi.com)

Histone gene expression and chromatin structure in mammalian cell hybrids. (rupress.org)

Notably, ZNF350 knockdown accelerated migration of the non-MG cells, while overexpression of ZNF350 in the MG cells significantly impaired their migration. (oncotarget.com)
This stress condition can also lead to the attraction of immune cells, notably antigen presenting cells (APCs) (i.e. immature dendritic cells), natural killer cells (NK cells), and cytotoxic T-cells to the site of infection [ 5 , 6 ]. (biomedcentral.com)

A promising new study for the future of neural stem cell (NSCs) from the University of Plymouth sheds light on how NSCs are activated to regenerate neurons and glial cells when damage or deterioration occurs. (rndsystems.com)
Dinorah Friedmann-Morvinski, a post-doctoral fellow in Inder Verma's lab at the Salk Institute, has shown how tumors originated from mature neurons dedifferentiate into glioma stem-like progenitor cells. (genengnews.com)
Administration of gene therapy viruses into skeletal muscle, where distal terminals of motor and sensory neurons reside, has been shown to result in extensive transduction of cells within the spinal cord, brainstem, and sensory ganglia. (frontiersin.org)
We then used a genetically-encoded hybrid voltage sensor (hVOS) to image voltage from a specific population of neurons. (stanford.edu)
This activity-induced gene enabled targeting of neurons that are activated when a mouse hears a pure 15 kHz tone. (stanford.edu)
By targeting a genetically-encoded hybrid voltage sensor (hVOS) to identify neurons activated during the presentation of sound, the properties and function of these neurons become accessible to study in slices of mouse ventral cochlear nucleus. (stanford.edu)

The median TBL1XR1 expression was remarkably diminished in FL, yet, intriguingly, GCB-subtypes of DLBCL-NOS exhibited significantly enhanced expression compared to FL (p=0.001). (iiarjournals.org)
The expression of the PgGRF gene was significantly upregulated under GA3 induction and three weeks of heat treatment. (biomedcentral.com)
All markers were significantly higher in hMSCs seeded on DMs or tDMs compared to cells on TCP. (ca.gov)

In the vertebrate CNS, HB9/MNR2 transcription factors are specifically expressed in all somatic motoneurons and are necessary to distinguish motoneurons from interneurons, in part by repressing interneuron-specific gene expression. (jneurosci.org)
On the basis of recent vertebrate studies, the HB9/MNR2 gene family is a prime candidate for a general determinant of somatic motoneuron cell type. (jneurosci.org)
Mouse HB9 maintains expression in somatic motoneurons, whereas chick HB9 is expressed only in postmitotic somatic motoneurons. (jneurosci.org)
Imprints entail the maintenance of germline-derived differential epigenetic marks, mostly in the form of DNA methylation, through to the somatic cells of the offspring. (nature.com)

We find that chromosomal rearrangements and related recombination defects are major but not sole causes of hybrid infertility. (elifesciences.org)
Fluorescence in situ hybridization (FISH) is a molecular pathological method with high sensitivity and specificity, which uses specific DNA probes to detect chromosomal aberrations, as well as gene deletion and amplification ( 21 , 22 ). (spandidos-publications.com)
The mouse biliary glycoprotein gene (Bgp): partial nucleotide sequence, expression, and chromosomal assignment. (shengsci.com)

Conclusion: Our exploratory findings unravel the expression patterns of TBL1XR1/NCOR1 in B-cell lymphoma variants. (iiarjournals.org)
the rest have above nonfunctional variants of the RHD gene. (medscape.com)

To characterize the effect of CH, primary rat CB cells were exposed to 7 days of CH, total RNA was extracted, cDNA-32P synthesized and hybridized with 1185 genes printed on a nylon membrane. (omicsdi.org)
Rb-MBG scaffold can load effectively and release Rb ions and ENX continuously to damage the bacterial cell membrane with the synergistic effect, and therefore achieve antibacterial results. (bvsalud.org)

The MG cells substantially lost their migratory properties after treatment with a methyltransferase inhibitor, 5-azacytidine, suggesting a role of DNA methylation in this process. (oncotarget.com)
Global methylation analysis revealed that 35 out of the 640 genes were hyper-methylated in the MG cells. (oncotarget.com)
Taken together, our results suggest that hyper-methylation of the ZNF350 proximal promoter may be one of the crucial determinants for acquiring increased migratory capabilities in colon cancer cells. (oncotarget.com)

The gene ontology and pathway enrichment of DEGs were performed by DAVID and Kyoto Encyclopedia of Genes and Genomes online analyses, respectively. (hindawi.com)
Traditional bioinformatics methods including gene ontology (GO) and pathways enrichment analyses of DEGs combined protein-protein interaction (PPI) network and cytohubber calculating software as well as Molecular Complex Detection (MCODE) analysis were used to analyze the key gene and pathway. (hindawi.com)

The TP53 gene mutation is the most common gene alteration in many tumors, including esophageal cancer. (spandidos-publications.com)
Patients usually do not increased incidence of oropharyngeal squamous cell carci- seek counseling until the tumors are large because small noma (OSCC), the head and neck cancer in which HPV is tumors cause little distress and may not be noticed by the most commonly found ( 4 ). (cdc.gov)

This determined how hierarchical interactions between regulatory elements orchestrate robust parent-specific expression, with implications for non-imprinted gene regulation. (nature.com)
We are driven by a desire to understand the molecular basis of organismal diversity, and the belief that many differences in physiology, morphology and behavior arise from changes in gene regulation. (berkeley.edu)
To provide a solid experimental foundation for our evolutionary studies, we are working with several other labs in Berkeley to systematically dissect gene expression and regulation in the early D. melanogaster embryo. (berkeley.edu)
Upon the stage of tumorigenesis, down-regulation of the major histocompatibility complex class-I (MHC-I) molecules and also the overexpression of the stress-induced ligands on the surface of malignant cells to avoid recognition by cytotoxic T-lymphocytes (CTLs), make them more sensitive to NK cell-mediated death. (biomedcentral.com)
This demonstrates that age-related constitutive elevations in stress response gene expression was accompanied by diminished SSC-induced responsiveness in epigenomic regulation and tissue remodeling. (cdc.gov)

Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
Thoracic aortic dissection (TAD) is a catastrophic disease worldwide, but the pathogenic genes and pathways are largely unclear. (hindawi.com)

In this study, transgenic hybrid poplar ( Populus alba × grandidentata ) was generated to express a hyperthermophilic endoglucanase from Thermotoga neapolitana with an optimal temperature over 100 °C. Functional hyperthermoactive endoglucanase was successfully produced in the transgenic events, and altered phenotypic growth was observed in transgenic lines. (biomedcentral.com)
The activation of Tn CelB by a post-harvest heat treatment resulted in enhanced saccharification efficiencies of transgenic poplar lines with moderate Tn CelB expression and without alteration of cellulose and lignin when not heat-treated. (biomedcentral.com)
Expression of hybrid cry3aM-licBM2 genes in transgenic potatoes ( Solanum tuberosum ). (evrogen.ru)

Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. (nature.com)
Our work indicates that parental origin of an epigenetic state is irrelevant as long as appropriate balanced gene expression is established and maintained at imprinted loci. (nature.com)
Epigenetic theory describes how changes in gene expression may be caused by mechanisms other than changes in the underlying DNA sequence, temporarily or through multiple generations, by influencing a network of chemical switches within cells collectively known as the epigenome. (modulusfe.com)

Since the evolutionary forces driving genetic changes that cause infertility between species are likely also acting within species, the study of hybrid sterility also promises significant insight into mechanisms underlying infertility within species. (elifesciences.org)
Although a number of workers have contributed to the concepts underlying genetic engineering based on recombinant DNA, Professor Stanley N. Cohen and his colleagues were the first to construct, in 1973, a biologically functional hybrid plasmid and in the following year to achieve actual expression of a foreign gene implanted in E. coli by the recombinant DNA method. (wolffund.org.il)
Dr. Cohen has exercised a central and seminal role in discovering and developing this methodology, and in demonstrating its usefulness for analysing complex genetic elements, and for obtaining expression of foreign genes in bacterial cells. (wolffund.org.il)
Virus-mediated gene therapy has the potential to deliver exogenous genetic material into specific cell types to promote survival and counteract disease. (frontiersin.org)
Crucially, some viral vectors (i.e., viruses specifically used to deliver genetic material into cells) have the potential to circumvent the blood-brain- (BBB) and blood-spinal cord barriers (BSCB) when intravenously injected. (frontiersin.org)
by "transduction," we mean the introduction of genetic material into target cells. (frontiersin.org)
[ 3 ] The Rh blood group system uses Fisher-Race nomenclature, and the Rh gene complex consists of 3 genetic loci each with 2 major alleles. (medscape.com)

Identifying the molecular and evolutionary bases of hybrid sterility is necessary for understanding the mechanisms of speciation. (elifesciences.org)
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
[3] Generally, the 155 aa/18 kDa low molecular weight (LMW) form is considered cytoplasmic and can be secreted from the cell, whereas the high molecular weight (HMW) forms are directed to the cell's nucleus. (wikidoc.org)

These results indicated that MgCu-MOF74 enhanced cellular viability, alkaline phosphatase levels, collagen (COL) synthesis and osteogenesis-related gene expression. (bvsalud.org)

In a recent study from the non-profit, Breast Cancer Now, a new potential therapeutic targeting cancer stem cells (CSCs) may have been uncovered. (rndsystems.com)
Redistribution of accumulated cell iron: a modality of chelation with therapeutic implications. (evrogen.ru)
Virus-based immunostimulatory cytokine and chemokine expression can recruit and activate T cells, antigen-presenting cells (APCs), and natural killer (NK) cells, and subsequently, improve the therapeutic activity of OVs. (biomedcentral.com)

Hybrid sterility is one of the earliest postzygotic isolating mechanisms to evolve between two recently diverged species. (elifesciences.org)
The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
The key is in how it interacts with cellular DNA, provide new insights into the mechanism highlight the importance of the protein stabilizing mechanisms that influence both gene control and protein quantities during developmental processes. (rndsystems.com)
These findings also emphasize the growing importance of single cell level studies to better understand these mechanisms. (rndsystems.com)
the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. (wikidoc.org)
In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
establish mechanisms of action by studying the effects of the same chemicals in experimental animals and on human cells in vitro, allowing for a better prediction of human carcinogenicity and assessment of carcinogenic mechanisms. (who.int)

In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. (biomedcentral.com)

This characteristic of the TP53 gene limits the use of IHC in TP53 gene research, which may be the cause of the conflicting results in the study of TP53 in esophageal cancer. (spandidos-publications.com)
Although high-throughput sequencing technologies have provided many diverse expressed genes, different expression profiles in TAD provided various results and no reliable results have been identified up to now [ 6 - 8 ]. (hindawi.com)
The results of this study may be helpful for further study of the function of PgGRF and PgGIF genes and lay a foundation for further study of their role in the growth and development of Panax ginseng . (biomedcentral.com)
The third form of Griscelli syndrome, whose expression is restricted to the characteristic hypopigmentation, results from mutation in the gene that encodes melanophilin, MLPH , the ortholog of the gene mutated in leaden mice. (medscape.com)
It has also been shown that an identical phenotype to Griscelli syndrome type 3 results from the deletion of the MYO5A F-exon, an exon with a tissue-restricted expression pattern. (medscape.com)
RESULTS: Gene array data indicated that Akt 1 and 2 transcript levels were decreased in old rats compared with young rats. (cdc.gov)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
The former leads to a stop codon in RHD gene and results in a lack of transcription product despite all intact exons. (medscape.com)

The chemi- appear to interact with the structural elements of the cell, cal analysis was assessed at DATA CHEM Laboratories using plasma- with apparent binding to the cytoskeleton [Porter et al. (cdc.gov)

Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were performed to detect the expression of p53 protein and gene deletion in ESCC tissue samples from different ethnic groups in Xinjiang, in order to analyze their association with clinicopathological characteristics and patient prognosis, as well as the sensitivity and specificity of the two methods. (spandidos-publications.com)

Transcriptome Analysis Reveals Inhibitory Effects of Lentogenic Newcastle Disease Virus on Cell Survival and Immune Function in Spleen of Commercial Layer Chicks. (escholarship.org)
The relative amount by which the average fitness of a POPULATION is lowered, due to the presence of GENES that decrease survival, compared to the GENOTYPE with maximum or optimal fitness. (bvsalud.org)

Transferred decellularized matrices (tDMs) were created by culturing human mesenchymal stem cells (hMSCs) on tissue culture plastic (TCP) under a controlled microenvironment to deposit a highly osteogenic DM, followed by collection, mechanical homogenization and transfer to a secondary culture surface. (ca.gov)

Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
An important tool for studying protein-protein interactions is the yeast two-hybrid system. (genome.gov)
In the yeast interactome study, the two-hybrid system was scaled up allowing researchers to catalog all protein-protein interactions within the yeast cell. (genome.gov)

While it is impractical to repeat every experiment done in D. melanogaster in every other strain and species, we are extending several classes of experiment to selected strains and species so that we can better understand regulatory variation at each of its multiple levels: how sequence variation affects binding, how binding variation affects expression, and how expression variation affects phenotype. (berkeley.edu)

Successful CRISPR/Cas9 mediated homologous recombination in a chicken cell line. (evrogen.ru)

one is used to bind to the DNA and the second is used to activate transcription of the gene. (genome.gov)
The DNA binding hybrid is a fusion protein that can bind to the reporter gene but cannot activate transcription. (genome.gov)
The transcription activation hybrid is a fusion protein that cannot activate expression of the reporter gene because it lacks the ability to bind to the DNA. (genome.gov)
When the bait protein interacts with a prey protein, the resulting complex can both bind to the reporter gene and activate its expression. (genome.gov)

Therefore, the overexpression of p53 does not always indicate a mutation in the TP53 gene. (spandidos-publications.com)
Overexpression of hyperthermophilic endoglucanase in feedstock had impacts on plant growth and cell wall composition, especially when the enzyme was highly expressed. (biomedcentral.com)

Kacar then synthesized that gene and inserted it into E. coli in place of the existing version. (uncommondescent.com)
The hybrid E. coli clearly suffered from the archaic component. (uncommondescent.com)
Within a couple of months - about 500 generations - the hybrid E. coli were growing as well as their modern counterparts. (uncommondescent.com)
Adding Rb can increase the activity of ALP, the secretion of VEGF and COLI, and the expression of HIF-1α of hBMSCs. (bvsalud.org)

For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)
Module analysis revealed that TAD was associated with significant pathways including cell cycle, vascular smooth muscle contraction, and adrenergic signaling in cardiomyocytes. (hindawi.com)
TBL1XR1 over-expression has been linked to poor prognosis in several solid organ cancers, impacting various oncogenic signaling pathways ( 6 ). (iiarjournals.org)

Although the biological significance of TP53 gene mutation is well characterized, its clinical significance in esophageal cancer remains controversial, especially as a prognostic biomarker. (spandidos-publications.com)

The hybrid inhibitor ameliorated obesity-induced kidney morphological and functional changes via decreasing kidney inflammation, fibrosis, oxidative stress, and renal injury. (omicsdi.org)
Gene array, RT-PCR, and morphological localization, distribution, and quantification of Akt, and its influence on young and old skeletal muscle of rats following chronic high-intensity mechanical loading via stretch-shortening contractions (SSCs) was assessed. (cdc.gov)

This is in contrast to control macaques, where less differentiated, interferon gamma-producing natural killer cells predominate. (nature.com)
It has been previously demonstrated that NK cell functionality is responsive to immunotherapies with IL-21 and interferon alpha (IFNα). (nature.com)
We provide evidence that such failure to resolve R-loops causes sustained activation of a systemic inflammatory response characterized by an interferon (IFN) gene expression signature. (uni-koeln.de)

Rh gene complex is described by 3 loci, and, therefore, 8 gene complexes are possible. (medscape.com)

The remaining distal part of the IG-DMR was shown to bind pluripotency transcription factors in mouse embryonic stem cells (mESCs), exhibit active enhancer marks (H3K27ac), and nascent transcription 18 , 19 . (nature.com)
Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

The TP53 gene plays an important role in regulating the cell cycle, apoptosis and DNA damage repair ( 8 , 9 ). (spandidos-publications.com)
Importantly, TBL1XR1's pronounced association with several DNA Damage repair (DDR) genes was noted suggesting influence on DNA repair. (iiarjournals.org)

Anatomy29

Organisms13

Diseases10

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes76

Disciplines and Occupations3

Information Science5

Health Care2

Differentially expressed genes6

Proteins26

Genomic5

Electron transport c1

Genome9

Chromosomes7

Tumor14

Mutations4

Transgene expression2

Phenotypes3

MRNA10

Regulates Gene Expression1

Subunit8

Chromosome2

Squamous cell carc6

Differentiation10

Induce3

Drosophila5

Sequences5

Assays3

Proliferation2

Accumulation4

Tissues7

Dlk12

Receptors4

Receptor3

Regulate2

Datasets2

0.051

Chromatin1

Notably2

Neurons6

Significantly3

Somatic4

Chromosomal3

Variants2

Membrane2

Methylation3

DEGs2

Tumors2

Regulation5

Pathogenic2

Transgenic3

Epigenetic3

Genetic7

Molecular3

Alkaline phosphatase1

Therapeutic3

Mechanisms7

Recombinant1

Results8

Interact1

Deletion1

Survival2

Mesenchymal stem1

Yeast3

Phenotype1

Homologous recombination1

Activate4

Overexpression2

Coli4

Pathways3

Mutation1

Morphological2

Interferon3

Loci1

Embryonic2

Damage2