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Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsFungal ProteinsGenes, FungalGene Expression Regulation, FungalSaccharomycesMolecular Sequence DataMutationRNA, FungalAmino Acid SequenceBase SequenceDNA, FungalChromosomes, FungalGenome, FungalCloning, MolecularPlasmidsGenetic Complementation TestSequence Homology, Amino AcidGene DeletionSuppression, GeneticTranscription, GeneticPhenotypeSpores, FungalDNA-Binding Proteinsbeta-FructofuranosidaseRestriction MappingTranscription FactorsGenes, SuppressorHaploidyMutagenesisVacuolesTransformation, GeneticFermentationRecombination, GeneticTemperatureDiploidyCell Cycle ProteinsKineticsMitochondriaSilent Information Regulator Proteins, Saccharomyces cerevisiaeRecombinant Fusion ProteinsSequence AlignmentSchizosaccharomycesProtein BindingGlucoseAllelesMembrane ProteinsMeiosisGenes, Mating Type, FungalDNA RepairSaccharomycetalesNuclear ProteinsKluyveromycesCDC28 Protein Kinase, S cerevisiaeSpheroplastsEscherichia coliAdenosine TriphosphatasesDNA PrimersRNA, MessengerGalactoseRecombinant ProteinsCell CycleRepressor ProteinsCell WallErgosterolCandida albicansKiller Factors, YeastBinding SitesPheromonesSubstrate SpecificityCarrier ProteinsOpen Reading FramesPromoter Regions, GeneticBiological TransportGenesSequence DeletionCulture MediaProtein Structure, TertiaryXyloseSirtuin 2Genes, LethalDNA ReplicationChitin SynthaseSchizosaccharomyces pombe ProteinsMembrane Transport ProteinsCathepsin AMitosisModels, BiologicalSequence Homology, Nucleic AcidChromosome MappingEthanolWineModels, GeneticCell NucleusConserved SequenceSpecies SpecificityTelomereMannosyltransferasesIndustrial MicrobiologyGenotype
S288CYeast Saccharomyces cerevisiaeBaker'sChromosomeExpressionProteinsStrainsOrganismDeletionOverexpressionSequencesMutationFermentationYeastsFunctionalTranscriptionRegulationHomology6000 genesGrowthCharacterizationMRNAMulticopyGenomesSecretoryBiosyntheticHomologousIsolationGenome-wideCloned and expressedWhole-genomeInositolTarget genesClonesSpeciesRegulateGeneticTranscriptionalVectorsSyntheticSequenceMutantAminoMutations
S288C3
  • Saccharomyces cerevisiae S288C Cox15p (COX15), partial mRNA. (genscript.com)
  • Saccharomyces cerevisiae S288C was used as a control strain. (springeropen.com)
  • A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. (nyu.edu)
Yeast Saccharomyces cerevisiae3
Baker's1
  • Saccharomyces cerevisiae (/ˌsɛrəˈvɪsi.iː/) (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). (wikipedia.org)
Chromosome4
Expression37
  • Using a randomized crossover study design, 16 adult pointer dogs were used to compare changes in fecal characteristics, oxidative stress marker concentrations, and gene expression when fed a SCFP-supplemented diet or control diet. (asas.org)
  • Blood cyclooxygenase-2 and myeloperoxidase mRNA gene expression was affected by diet during transport stress, being increased in control dogs, but stable or decreased in dogs fed SCFP. (asas.org)
  • The rationale for the isolation of such genes was that their promoters may be useful in a biotechnological application, where the regulated expression of heterologous proteins is required. (le.ac.uk)
  • We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. (springeropen.com)
  • Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. (springeropen.com)
  • Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. (springeropen.com)
  • Analyses of genes whose expression is induced by high temperature are important for understanding the mechanisms of thermotolerance, and for the use of the genes in breeding thermotolerant yeasts. (springeropen.com)
  • In the present study, we attempted to identify genes whose expression is correlated with the degree of thermotolerance in S. cerevisiae . (springeropen.com)
  • We compared the gene expression profiles of three S. cerevisiae strains that showed different levels of thermotolerance. (springeropen.com)
  • Engineering dynamic, environmentally- and temporally-responsive control of gene expression is one of the principle objectives in the field of synthetic biology. (biomedcentral.com)
  • Using sucrose as an activator of gene expression circumvents the need for expensive inducer compounds and enables gene expression to be triggered during growth on a fermentable, high energy-yield carbon source. (biomedcentral.com)
  • The ability to fine-tune the timing and population density at which gene expression is activated from the SUC2 promoter was demonstrated by varying the ratio of glucose to sucrose in the growth medium. (biomedcentral.com)
  • Finally, we demonstrated that the system could also be used to repress gene expression (a process also required for many engineering projects). (biomedcentral.com)
  • We used the glucose/sucrose system to control a heterologous RNA interference module and dynamically repress the expression of a constitutively regulated GFP gene. (biomedcentral.com)
  • The capacity to repress gene expression using RNA interference makes the system highly versatile, with great potential for metabolic engineering applications. (biomedcentral.com)
  • Microorganisms possess a variety of mechanisms for modulating gene expression levels according to changes in their environment. (biomedcentral.com)
  • One of the most promising techniques to optimise cell factory performance is to control the expression/repression of relevant genes using dynamic regulation. (biomedcentral.com)
  • Previous efforts to implement dynamic regulation under industrially relevant conditions required the knockout of galactose utilization genes so that a small amount of galactose can be added to fermentations as a gratuitous inducer for gene expression [ 6 ]. (biomedcentral.com)
  • High concentrations of fermentable carbon sources repress gene expression from galactose promoters (GAL) via a carbon catabolite repression mechanism [ 7 ], and galactose utilisation genes are not activated during growth on sucrose [ 8 ]. (biomedcentral.com)
  • These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. (nyu.edu)
  • It is beneficial to improve the protein expression and product yield by controlling the promoter strength to fine regulate the transcription level of the target gene and maximize the transcription efficiency. (biomedcentral.com)
  • Ume6p was found to be a positive regulator of PIS1 gene expression. (umass.edu)
  • Saccharomyces cerevisiae showed only minor alterations in growth and gene expression when its 16 chromosomes were combined. (the-scientist.com)
  • The expression analysis of both gene family members reflect their preferential seed-specific expression, especially during the mature embryo development stage. (nature.com)
  • A curated database of genes associated with dietary restriction in model organisms either from genetic manipulation experiments or gene expression profiling. (senescence.info)
  • Projects focused on gene expression profiling of ageing and of dietary manipulations of ageing, such as caloric restriction. (senescence.info)
  • NCOR2 expression and its occupancy on peroxisome proliferator-activated receptor (PPAR) target gene promoters are increased with age in major metabolic tissues. (senescence.info)
  • Shifting its repressive activity towards PPARs, by selectively disabling one of its two major receptor-interacting domains, resulted in premature ageing in mice and related metabolic diseases accompanied by reduced mitochondrial function and antioxidant gene expression. (senescence.info)
  • Central to this investigation is an understanding of what gene products are required and expressed during a natural infection and how this expression changes over time (from initial colonization to causation of disease and spread of the pathogen to new hosts) and space (in different cells or tissues within the host). (cdc.gov)
  • Although analyses that give information on the expression of a few genes provide insight and have been responsible for a large proportion of the bacterial pathogenesis literature currently available, our ultimate goal is to understand expression changes across the whole genome. (cdc.gov)
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
  • Two of these methods, signature-tagged mutagenesis and in vivo expression technology, do not directly measure gene expression and do not allow true genomic-scale analysis, but they have been devised to identify genes necessary for pathogens during real infections. (cdc.gov)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae. (bvsalud.org)
  • states'' or gene expression patterns. (lu.se)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Proteins6
  • Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. (wikidata.org)
  • The interactions of proteins with chromosomal DNA control a variety of cellular processes, including gene transcription, DNA packing, replication, recombination, and DNA repair. (psu.edu)
  • Furthermore, their overexpression in wild‐type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that they may be rate‐limiting factors in this process. (vtt.fi)
  • The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. (vtt.fi)
  • In accordance with the high homology level of the secretory pathway proteins in different yeast species, the polyclonal antibodies raised against S. cerevisiae Seb1p, Sso2p and Sec4p can detect homologous proteins in cell lysates of K. lactis and Pichia pastoris, the latter also in Candida utilis. (vtt.fi)
  • To implement this system, three orthogonal Cas proteins were utilized: dLbCpf1 fused to a transcriptional activator, dSpCas9 fused to a transcriptional repressor, and SaCas9 for gene deletion. (illinois.edu)
Strains5
  • The intestine of Polistes dominula, a social wasp, hosts S. cerevisiae strains as well as S. cerevisiae × S. paradoxus hybrids. (wikipedia.org)
  • 2016) showed that the intestine of Polistes dominula favors the mating of S. cerevisiae strains, both among themselves and with S. paradoxus cells by providing environmental conditions prompting cell sporulation and spores germination. (wikipedia.org)
  • The growth properties of S. cerevisiae at high temperatures differ according to the strain used, but the optimum temperature of proliferation is around 30°C. Some high-temperature-resistant strains have been isolated from tropical areas and obtained by mutational improvement, breeding, or the cell fusion of existing strains (Pasha et al. (springeropen.com)
  • Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. (nyu.edu)
  • We conducted a multicopy suppressor screen for genes that suppress the loss of silencing in strains overexpressing Mec1p. (uky.edu)
Organism1
  • Saccharomyces cerevisiae (often known as budding yeast, or brewers yeast) is a single-celled micro- organism that is easy to grow and genetically manipulate. (lu.se)
Deletion3
  • 2003 ]) and a genome-wide screening of an S. cerevisiae deletion mutant collection (Auesukaree et al. (springeropen.com)
  • A wild-type strain, S. cerevisiae BY4743 and the deletion strain fmp21 Δ derived from BY4743 were obtained from EUROSCARF (EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis). (springeropen.com)
  • Consequently, the WGD scenario requires that almost an entire gene complement be deleted, and that deletion within regions (termed 'blocks') establishing DS patterns proceed with precision and without leaving massive evidence of relics (gene remnants) or pseudogenes (Fig. 1A ). (biomedcentral.com)
Overexpression4
  • Overexpression of these genes can rescue temperature‐sensitive (ts) growth defect of many sec mutants impaired in protein secretion. (vtt.fi)
  • Design and construction of an optimal microbial cell factory typically requires overexpression, knockdown, and knockout of multiple gene targets. (illinois.edu)
  • RNA-sequencing analysis showed that the overexpression of differential gene HSP30 increased the transcriptional abundance of ATF1 gene and production of ethyl acetate. (biomedcentral.com)
  • Furthermore, the results proved that the Hsf1, an upstream transcription factor of Hsps, had a positive effect on alleviating the unfolded protein response and that overexpression of Hsf1 reprogramed the pattern of ATF1 gene transcript levels. (biomedcentral.com)
Sequences2
  • The following COX15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • We focus on (1) pairwise comparison of gene arrangement sequences in A. gossypii and S. cerevisiae , (2) reconstruction of gene arrangements ancestral to A. gossypii , S. cerevisiae , and K. waltii , (3) synteny patterns arising within and between lineages, and (4) expected gene orientation of duplicate gene sets. (biomedcentral.com)
Mutation2
Fermentation2
Yeasts1
Functional2
  • Replacing yeast genes with their human equivalents reveals functional conservation despite a billion years of divergent evolution. (the-scientist.com)
  • Our research also focuses on the synthesis mechanisms of telomerase, and aims for the identification and functional analysis of the genes that regulate telomerase. (lu.se)
Transcription9
  • Transcription of these six genes has been examined under a variety of growth conditions and in various mutant backgrounds. (le.ac.uk)
  • In synthetic biology, the strength of promoter elements is the basis for precise regulation of target gene transcription levels, which in turn increases the yield of the target product. (biomedcentral.com)
  • However, the results of many researches proved that excessive transcription levels of target genes actually reduced the yield of the target product. (biomedcentral.com)
  • In this study, promoters PGK1p and TDH3p with different strengths were used to regulate the transcription level of alcohol acetyl transferase encoding gene ATF1 . (biomedcentral.com)
  • Hsp26, Hsp78, Hsp82) decreased the production of ethyl acetate, suggesting that the Hsp family was also involved in the regulation of ATF1 gene transcription. (biomedcentral.com)
  • The fine-tuning and precise control of key gene transcription levels are the important basis for the successful implementation of these strategies. (biomedcentral.com)
  • Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p. (umass.edu)
  • In Saccharomyces cerevisiae, transcription of most of the phospholipid biosynthetic genes (e.g. (umass.edu)
  • String-based protein-protein interaction analysis suggested that bZIP53, a transcription factor might be involved in the activation of transcription of ELO / KCS genes. (nature.com)
Regulation5
  • Title: Regulation of the heme A biosynthetic pathway: differential regulation of heme A synthase and heme O synthase in Saccharomyces cerevisiae. (genscript.com)
  • Ribosomes have been suggested to directly control gene regulation, but regulatory roles for ribosomal RNA (rRNA) remain largely unexplored. (nih.gov)
  • Together, these studies unravel unexpected gene regulation directly mediated by rRNA and how ribosome evolution drives translation of critical developmental regulators. (nih.gov)
  • Thus, we demonstrate novel regulation of the PIS1 gene by Ume6p. (umass.edu)
  • FAE ( Fatty Acid Elongation ) like genes take part in the biosynthesis of VLCFAs, growth regulation, and stress responses, and are further comprised of KCS ( Ketoacyl-CoA synthase ) and ELO ( Elongation Defective Elongase ) sub-gene families. (nature.com)
Homology2
  • In both studies, regions of 'double synteny' (DS) were identified in which single genes or groups of genes expressed homology relationships with alternating chromosomal regions of S. cerevisiae . (biomedcentral.com)
  • Although the vast majority of protein-encoding genes (95%) show homology to S. cerevisiae genes, only ~10% are gene duplicates in DS patterns. (biomedcentral.com)
6000 genes1
Growth6
  • The optimum temperature for growth of S. cerevisiae is 30-35 °C (86-95 °F). Two forms of yeast cells can survive and grow: haploid and diploid. (wikipedia.org)
  • Both of the thiamine genes, THI4 and THI5, are subject to repression by exogenous thiamine and become derepressed on depletion of the vitamin from the growth medium. (le.ac.uk)
  • Genomic regions related to the high-temperature growth of S. cerevisiae were identified by quantitative trait locus (QTL) mapping (Steinmetz et al. (springeropen.com)
  • The QTL map contained MKT1 , END3 , and RHO2 as high-temperature growth quantitative trait genes. (springeropen.com)
  • URA3 null mutant exhibits growth characteristics typical of other VMA disruptant mutants in genes encoding subunits of the catalytic sector. (tau.ac.il)
  • Starting from simple modelling of individual growth curves, a Bayesian hierarchical model can be built with variable selection indicators for inferring pairs of genes that genetically interact. (lu.se)
Characterization1
MRNA1
  • The PRP17 gene product is required for the second step of pre-mRNA splicing reactions. (iisc.ac.in)
Multicopy2
  • Petes, Thomas D. / The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy suppressor of mec1 telomeric silencing defects . (uky.edu)
  • Craven, RJ & Petes, TD 2001, ' The Saccharomyces cerevisiae suppressor of choline sensitivity (SCS2) gene is a multicopy suppressor of mec1 telomeric silencing defects ', Genetics , vol. 158, no. 1, pp. 145-154. (uky.edu)
Genomes1
  • With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
Secretory2
  • S. cerevisiae is currently the only yeast cell known to have Berkeley bodies present, which are involved in particular secretory pathways. (wikipedia.org)
  • The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. (vtt.fi)
Biosynthetic2
  • A further two, previously undescribed genes, encode a second small, 12kDa, stress-induced protein (HSP12) and a thiamine biosynthetic enzyme (THI4). (le.ac.uk)
  • DNA sequence analysis revealed the THI5 gene to encode a second thiamine biosynthetic enzyme homologous to the product of the Schizosaccharomyces pombe gene nmtl. (le.ac.uk)
Homologous1
  • When a 14-kDa polypeptide was suggested to be a subunit of a specialized V-ATPase in Manduca sexta, and a homologous short sequence was detected downstream of the UGA1 gene in yeast, we cloned this counterpart gene from yeast. (tau.ac.il)
Isolation2
  • Here we report isolation of Kluyveromyces lactis homologues of these genes. (vtt.fi)
  • The additional information generated by whole genome studies goes far beyond that derived by characterizing in isolation more genes and gene products, because analysis of the whole genome allows complete regulatory networks to be identified and characterized. (cdc.gov)
Genome-wide1
  • The comparative genome-wide analysis and mode of evolution of KCS and ELO gene families have not been investigated in tetraploid Brassica carinata and its diploid progenitors. (nature.com)
Cloned and expressed1
Whole-genome3
  • Recent comparative genomic studies claim local syntenic gene-interleaving relationships in Ashbya gossypii and Kluyveromyces waltii are compelling evidence for an ancient whole-genome duplication event in Saccharomyces cerevisiae . (biomedcentral.com)
  • Furthermore, phylogenetic trees reconstructed under alternative hypotheses placed the putative whole-genome duplication event after the divergence of the S. cerevisiae and K. waltii lineages, but in the lineage leading to K. waltii . (biomedcentral.com)
  • The existence of an ancient whole-genome duplication (WGD) event in Saccharomyces cerevisiae [ 1 ] has been debated over the past several years. (biomedcentral.com)
Inositol1
  • We identified SCS2 (suppressor of choline sensitivity), a gene previously isolated as a suppressor of defects in inositol synthesis. (uky.edu)
Target genes1
  • NCOR2, also known as SMRT, is a transcriptional corepressor that maintains the transcriptional silencing of certain target genes. (senescence.info)
Clones1
  • Six cDNA clones, which showed differential hybridisation to mRNAs of exponential and stationary phase cells, were used as probes to recover the cognate genes from a library of yeast genomic DNA fragments carried in the bacteriophage vector, EMBL3. (le.ac.uk)
Species1
  • GENE-UP® BREWPRO™ BACTERIA PLUS provides genomic-level detection of the lactic-acid bacteria species paired with risk-based detection by targeting the horA/horC hop resistance genes in the same assay. (biomerieux.com)
Regulate1
  • Because the environment encountered within a living host will be quite different from the external environment, pathogens must be able to regulate the necessary genes in coordination as they move from the environment to the host and from one host niche to another. (cdc.gov)
Genetic1
Transcriptional2
  • Therefore the aims of this project were to identify the unknown members of this set of genes and to determine the transcriptional regulatory mechanisms acting on all six. (le.ac.uk)
  • Thus, although these genes show apparent coordinate induction they are regulated via different transcriptional control mechanisms. (le.ac.uk)
Vectors1
  • We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. (nyu.edu)
Synthetic1
  • Synthetic biologists introduce bacterial and poppy plant genes into yeast to manufacture morphine. (the-scientist.com)
Sequence2
  • A single transcript chosen for a gene which is the most conserved, most highly expressed, has the longest coding sequence and is represented in other key resources, such as NCBI and UniProt. (ensembl.org)
  • DNA sequence analysis carried out in this study has characterised the two remaining genes as HXT4=LGT1, a low affinity hexose transporter and a new thiamine regulated gene, THI5. (le.ac.uk)
Mutant1
  • However, a viable nonsense mutant has been isolated for the prfB gene of S. typhimurium encoding RF2 release factor. (biomedcentral.com)
Amino1
  • Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change. (wikidata.org)
Mutations6
  • Nonsense mutations in the essential gene SUP45 can be isolated in the absence of tRNA nonsense suppressors. (biomedcentral.com)
  • Also it has been shown that nonsense mutations (UAA) in the SUP45 gene lead to lethality in the absence of SUQ5 suppressor [ 29 ]. (biomedcentral.com)
  • Only mutations in the N-terminal nonconserved domain of PRP17 are synthetically lethal in combination with mutations in PRP16 and PRP18, two other gene products required for the second splicing reaction. (iisc.ac.in)
  • ALG6 -CDG is caused by mutations in the ALG6 gene. (medlineplus.gov)
  • ALG6 gene mutations lead to the production of an abnormal enzyme with reduced or no activity. (medlineplus.gov)
  • which means both copies of the gene in each cell have mutations. (medlineplus.gov)