• Cancer-associated fibroblasts (CAFs) are one major type of component identified in the tumor microenvironment. (spandidos-publications.com)
  • Fibroblast activation protein (FAP) is overexpressed in cancer-associated fibroblasts and is involved in a variety of tumor-promoting activities such as matrix remodeling, angiogenesis, chemotherapy resistance, and immunosuppression. (snmjournals.org)
  • Cancer-associated fibroblasts modulate the microenvironment of malignant tumors by secreting factors that regulate both malignant cells and nonmalignant cells such as immune and endothelial cells ( 1 ). (snmjournals.org)
  • Although heterogeneous in their origin, cancer-associated fibroblasts have common properties distinct from normal fibroblasts and show expression of proteins not found in their normal counterparts. (snmjournals.org)
  • Cancer‑associated fibroblasts (CAFs) are pivotal in tumor progression. (spandidos-publications.com)
  • The tumor microenvironment consists of various cell types and fibroblasts in the tumor tissue, called cancer-associated fibroblasts (CAFs), which play pivotal role in tumor progression ( 7 , 8 ). (spandidos-publications.com)
  • While cancer-associated fibroblasts (CAFs) are increasingly appreciated as covering a spectrum of perturbed states, we lack a coherent understanding of their relationship-if any-with the steady-state fibroblast subtypes. (lu.se)
  • Prognostic value of genes related to cancer-associated fibroblasts in lung adenocarcinoma. (cdc.gov)
  • The effects of 13-cis retinoic acid (RA) and dexamethasone on the levels of epidermal growth factor (EGF) receptor and fibroblast derived proteoglycan core protein (PG40) mRNAs were studied in human skin fibroblasts. (medicaljournals.se)
  • RESULTS: Irrespective of disease process, synovial fibroblasts constitutively produced higher levels of IL-6 and monocyte chemoattractant protein 1 (MCP-1) (CCL2) than skin fibroblasts. (ox.ac.uk)
  • Seven genes were differentially expressed in synovial fibroblasts compared with skin fibroblasts. (ox.ac.uk)
  • Three other genes (aggrecan, biglycan and caldesmon) were expressed at higher levels in all types of synovial fibroblasts compared with skin fibroblasts even after stimulation with TNF-alpha and IL-1. (ox.ac.uk)
  • In the absence of exogenous cytokine, synovial and skin fibroblasts exhibited similar patterns of gene expression. (birmingham.ac.uk)
  • Interestingly, the transcriptional response of skin fibroblasts to IL-4 converged with that of TNF-alpha-treated synovial fibroblasts, suggesting resident tissue fibroblasts and their blood-borne precursors may be imprinted by inflammatory cytokines that are characteristic of different tissues. (birmingham.ac.uk)
  • Therefore, we examined here intracellular AA levels and stability throughout its exposure to human skin fibroblasts in vitro. (nih.gov)
  • Thus, these results indicate that human skin fibroblasts exposed to AA over time had rising levels of type 1/type 4 collagens and SVCT2 mRNA expression and type 1 procollagen synthesis. (nih.gov)
  • Cell cycle synchronization and analysis of apoptosis‐related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna). (logosbio.com.cn)
  • In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1 Silencing Transcription Factor (REST) inhibitn together with a combination of GABAergic fate determinants to convert human adult skin fibroblasts into GABAergic neurons. (lu.se)
  • FALDH or FAO activity is measured in cultured skin fibroblasts or leukocytes. (medscape.com)
  • Patients who are affected usually have less than 10% of the normal mean FALDH activity in cultured skin fibroblasts, and obligate Sjögren-Larsson syndrome heterozygotes demonstrate reduced enzyme activity to about 50% of the normal value. (medscape.com)
  • The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. (nih.gov)
  • Fibroblast growth factor receptor-like 1 is a protein that in humans is encoded by the FGFRL1 gene. (wikipedia.org)
  • The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. (wikipedia.org)
  • The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. (wikipedia.org)
  • Fibroblasts are most commonly found in connective tissue that synthesizes extra-cellular matrix and another main structural protein of animals, collagen. (medindia.net)
  • Of these genes, four [tissue factor pathway inhibitor 2 (TFPI2), growth regulatory oncogene beta (GRObeta), manganese superoxide dismutase (MnSOD) and granulocyte chemotactic protein 2 (GCP-2)] were all found to be constitutively overexpressed in synoviocytes derived from patients with osteoarthritis. (ox.ac.uk)
  • Of the 31 genes, the 21 upregulated genes were primarily associated with cell paracrine and intracellular signaling, transcription regulation and cell adhesion and migration, and their transcriptional products included transforming growth factor-β2 (TGF-β2), insulin-like growth factor-binding protein 2 and transcriptional factor AP-2α/γ ( 11 ). (spandidos-publications.com)
  • One of these proteins is the fibroblast activation protein (FAP), a type II transmembrane serine protease with both dipeptidyl peptidase activity and endopeptidase activity ( 2 - 4 ). (snmjournals.org)
  • The LMNA protein was nearly absent in ~80% of the cardiac fibroblasts and ~25% of cardiac myocytes in the Pdgfra-CreLmna F/F mice . (bvsalud.org)
  • Diphenylhydantoin plays a role in gene expression related to cytoskeleton and protein adhesion in human normal palate fibroblasts « Dott. (carinci.org)
  • The FGF8 gene provides instructions for making a protein called fibroblast growth factor 8 (FGF8). (medlineplus.gov)
  • This protein is part of a family of proteins called fibroblast growth factors that are involved in many processes, including cell division, regulation of cell growth and maturation, and development before birth. (medlineplus.gov)
  • FGF8 attaches (binds) to another protein called fibroblast growth factor receptor 1 (FGFR1) on the cell surface, which triggers a cascade of chemical reactions inside the cell. (medlineplus.gov)
  • Most of the FGF8 gene mutations that cause Kallmann syndrome change single protein building blocks (amino acids) in the FGF8 protein. (medlineplus.gov)
  • Our results show a successful conversion in 25 days with upregulation of neuronal gene and protein expression levels. (lu.se)
  • FGF2 , also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene . (wikidoc.org)
  • Fibroblast growth factor protein was first purified in 1975, but soon afterwards others using different conditions isolated basic FGF, Heparin-binding growth factor-2, and Endothelial cell growth factor-2. (wikidoc.org)
  • Gene sequencing revealed that this group was in fact the same FGF2 protein and that it was a member of a family of FGF proteins. (wikidoc.org)
  • Basic fibroblast growth factor has been shown to interact with casein kinase 2, alpha 1 , [13] RPL6 [14] and ribosomal protein S19 . (wikidoc.org)
  • The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. (nih.gov)
  • In contrast to protein interaction networks, enriched gene ontology (GO) terms of AMI related genes were used to construct a gene ontology interaction (GOI) network, which can be used to simulate the functional interactions between differential expressed genes of disease. (frontiersin.org)
  • This loss is accompanied with an increased expression of the histone demethylase Jmjd3 and with the recruitment of the MLL1 protein, and correlates with the expression of the Ink4a/Arf genes. (plos.org)
  • APJ‑suppressed fibroblasts treated with an antagonist or small interfering RNA showed myofibroblast‑like properties, including increased proliferation and migratory abilities, via accelerated phosphorylation of Sma‑ and Mad‑related protein 2/3 (Smad2/3). (spandidos-publications.com)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
  • This study evaluated the effect of matrix metalloproteinases-2 (MMP-2) gene silencing using MMP-2 shRNA expression plasmids (pMMP-2) on IR induced cytotoxicity and DNA damage by MTT, dead green, γH2AX and comet assays in human normal dermal fibroblasts (HDFs) and MCF-7 human breast cancer cells. (biomedcentral.com)
  • The aim of the present investigation is to study the effect of MMP-2 gene silencing by transfection of MMP-2 shRNA expression plasmids (pMMP-2on radiation-induced DNA damage in human normal dermal fibroblasts (HDFs) and MCF-7 cells. (biomedcentral.com)
  • stopping stress-induced skin maturing and claim that its results are linked to the inhibition of GR signaling in individual dermal fibroblasts. (president2010.info)
  • Ionone Does not have any Influence on the Viability of Dexamethasone-Untreated and Dexamethasone-Treated Individual Dermal Fibroblasts To determine whether -ionone impacts cell viability, we performed the WST-1 assay in individual dermal fibroblasts which were either treated or neglected with dexamethasone. (president2010.info)
  • At concentrations to 200 M up, -ionone got no influence in the viability of dexamethasone-untreated and dexamethasone-treated individual dermal fibroblasts (Body 1a,b). (president2010.info)
  • Open up in another window Body 1 (a,b) Ramifications of -ionone in the viability of dexamethasone-untreated and dexamethasone-treated individual dermal fibroblasts. (president2010.info)
  • Ionone Attenuates Dexamethasone-Induced Suppression of Collagen Synthesis in Individual Dermal Fibroblasts To research whether -ionone can boost collagen creation in dexamethasone-treated individual dermal fibroblasts, we quantified procollagen type I C-peptide in the lifestyle moderate and gene appearance degrees of collagen type I 1 string (in individual dermal fibroblasts. (president2010.info)
  • In keeping with this total result, -ionone considerably elevated the gene appearance degrees of and in the dexamethasone-treated individual dermal fibroblasts (Body 2b). (president2010.info)
  • Open up in another window Body 2 Influence of -ionone on collagen synthesis in individual dermal fibroblasts. (president2010.info)
  • The cells had been treated with either automobile (proven as -) or three doses of -ionone (12.5, 25, or 50 M (shown as +)) and dexamethasone (1 M) for 24 h. (a) The procollagen type I C-peptide articles was assessed in the lifestyle supernatants from the dermal fibroblasts, and (b,c) the gene appearance degrees of collagen type I 1 string (had been quantitated in the cells. (president2010.info)
  • Method: We examined that recombinant human bFGF affects the expression of genes involved in ECM synthesis and remodeling in human dermal fibroblasts cultures as judged by Northern blot analysis. (dsmc.or.kr)
  • Conclusion: The type Ⅰ and Ⅶ collagen gene expression in dermal fibroblasts is clearly subjected to modulation by the cytokines including bFGF with uncoordinate regulatory pathway. (dsmc.or.kr)
  • Chicken interferome: avian interferon-stimulated genes identified by microarray and RNA-seq of primary chick embryo fibroblasts treated with a chicken type I interferon (IFN-α). (essex.ac.uk)
  • This study focusses on the potentially anti-viral genes by identifying those induced just by interferon in primary chick embryo fibroblasts. (essex.ac.uk)
  • Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts. (who.int)
  • Detection of differentially expressed genes in synovial fibroblasts by restriction fragment differential display. (ox.ac.uk)
  • OBJECTIVE: To identify differentially expressed genes in synovial fibroblasts and examine the effect on gene expression of exposure to TNF-alpha and IL-1beta. (ox.ac.uk)
  • Differential gene expression was confirmed by reverse transcription-polymerase chain reaction and immunohistochemistry using a variety of synovial fibroblasts, including cells from patients with osteoarthritis and self-limiting parvovirus arthritis. (ox.ac.uk)
  • These four genes were only weakly expressed in other synovial fibroblasts (rheumatoid and self-limiting parvovirus infection). (ox.ac.uk)
  • While these differences may represent differential behaviour of synovial fibroblasts in in vitro culture, these observations suggest that TFPI2, GRObeta (CXCL2), MnSOD and GCP-2 (CXCL6) may represent new targets for treatments specifically tailored to osteoarthritis. (ox.ac.uk)
  • Co-culture of skin or synovial fibroblasts with synovial fluid-derived mononuclear cells provided further evidence that these transcriptional differences were functionally significant in an ex vivo setting. (birmingham.ac.uk)
  • Adenoviral gene transfer of the endogenous inhibitor IkappaBalpha into human osteoarthritis synovial fibroblasts demonstrates that several matrix metalloproteinases and aggrecanases are nuclear factor-kappaB-dependent. (ox.ac.uk)
  • OBJECTIVE: To investigate the role of the transcription factor nuclear factor-kB (NF-kappaB) in promoting inflammatory and destructive responses in human osteoarthritis (OA) synovial fibroblasts, by assessing the effect of NF-kappaB blockade on the production of cytokines and destructive enzymes. (ox.ac.uk)
  • CONCLUSION: In OA synovial fibroblasts, inhibition of NF-kappaB has a beneficial effect on the balance between the expression of proinflammatory cytokines and antiinflammatory mediators. (ox.ac.uk)
  • By contrast, the 10 downregulated genes were primarily associated with epithelial membrane proteins ( 11 ). (spandidos-publications.com)
  • Moreover, expression of senescence -associated beta-galactosidase was induced and levels of the senescence-associated secretory phenotype (SASP) proteins TGFß1, CTGF (CCN2), and LGLAS3 were increased as well as the transcript levels of additional genes encoding SASP proteins in the Pdgfra-CreLmna F/F mouse hearts . (bvsalud.org)
  • Deletion of the Lmna gene in fibroblasts partially recapitulates the phenotype of the LMNA-associated DCM, likely through induction of double-stranded DNA breaks , activation of the DDR pathway, and induction of expression of the SASP proteins . (bvsalud.org)
  • [3] [5] FGF2 binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins which themselves constitute a family of closely related molecules. (wikidoc.org)
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
  • Standing between a cancer cell in situ and the surrounding tissue of fibroblasts and extra-cellular matrix is the basement membrane, a thin sheet of fibers that normally cradles the cells above it. (medindia.net)
  • do cancer cells invade the basement membrane or do some fibroblasts help invading cancers? (medindia.net)
  • Using human colon cancer cells and primary human fibroblasts isolated from tumors and adjacent normal tissues, Alexandros Glentis and colleagues at the Institut Curie addressed the question of whether the cancer cells or the CAF cells were responsible for the breakdown of the basement membrane that leads to cancer progression. (medindia.net)
  • A combination of markers was used to discriminate fibroblasts from other cell types to validate the purity of isolated cells and to discriminate NAFs from CAFs. (medindia.net)
  • The researchers then devised an assay that deployed native basement membrane to separate cancer cells on one side and fibroblasts embedded in collagen on the other. (medindia.net)
  • Through imaging the tumors and fibroblasts, the researchers then found the smoking gun of cancer-CAF cooperation--long protrusions like puppet-strings that the tumor cells used to communicate with the fibroblasts well before the cancer cells moved to breach the basement membrane. (medindia.net)
  • The findings indicate that the phenotype in the LMNA-associated DCM is the aggregate consequence of the LMNA deficiency in multiple cardiac cells , including cardiac fibroblasts . (bvsalud.org)
  • The mRNA gene expression of ADAMTS4 was also inhibited by IkappaBa overexpression, particularly in IL-1-stimulated cells, but ADAMTS5 was unaffected. (ox.ac.uk)
  • In addition, MMP-2 gene silencing using pMMP-2 decreased comet tail length in irradiated HDFs but increased in irradiated MCF-7 cells. (biomedcentral.com)
  • In earlier analyses from the same ONS cells and fibroblasts we found zero patient-control differences in gene expression or cell functions that may be ascribed to medication or smoking cigarettes.15 Our findings is highly recommended preliminary, being predicated on only a small amount of people. (mdm-inhibitors.com)
  • gene manifestation, with Gene Ontology evaluation displaying how the affected genes clustered in systems connected with cell development differentially, proliferation, and motion, functions regarded as affected in schizophrenia patient-derived cells. (mdm-inhibitors.com)
  • Olfactory neurosphere-derived (ONS) cells and fibroblasts offer comparison between schizophrenia-associated DNA methylation in adult cells from neural and non-neural roots. (mdm-inhibitors.com)
  • A second goal was to determine whether schizophrenia requires DNA methylation that's transported into adulthood, exemplified by patient-derived ONS fibroblasts and cells. (mdm-inhibitors.com)
  • These seeks had been attained by obtaining genome-wide DNA gene and methylation manifestation profiles from iPS cells, ONS cells, and fibroblasts through the same individuals, and controls had been obtained as well as the schizophrenia-associated genes had been subjected to practical annotation and pathway evaluation to recognize affected cell features and processes. (mdm-inhibitors.com)
  • How big is the represents the magnitude of difference in gene expression between control-derived and patient-derived cells. (mdm-inhibitors.com)
  • Network analysis confirmed p53 and NF-κB transcription factor-regulated gene clusters in irradiated and bystander cells and suggested novel regulators, such as KDM5B/JARID1B (lysine (K)-specific demethylase 5B) and HDACs (histone deacetylases), which could epigenetically coordinate gene expression after irradiation. (biomedcentral.com)
  • Although interferon was discovered in chicken cells, details of the response, particularly the identity of hundreds of stimulated genes, are far better described in mammals. (essex.ac.uk)
  • Bolandi Z, Ghanbarian H. Increasing the specific expression of Cdk9 gene by single-stranded adult microRNA-1 in fibroblast cells. (ac.ir)
  • Methods: Mouse fibroblast cells were cultured in DMEM containing 10% FBS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin. (ac.ir)
  • Results: miR-1-expressing plasmid (pPRO-miR-1) was transfected into fibroblast cells. (ac.ir)
  • Interestingly, transfection of synthetic 22 nucleotide mature single stranded miR-1 (miR-1-3p) induced Cdk9 expression in the fibroblast cells. (ac.ir)
  • We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
  • Using this technique, previous studies have shown that γ-aminobutyric acid (GABA) expressing interneurons can be generated from different cell sources, such as glia cells or fetal fibroblasts. (lu.se)
  • Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
  • A new study shows that an enzyme involved in appropriately repressing sets of neuronal genes in non-neuronal cells is also a target for certain monoamine oxidase inhibitors used to treat depression. (sciencedaily.com)
  • In 2005, professor Ramin Shiekhattar, Ph.D., at The Wistar Institute and his colleagues reported details about an enzyme involved in appropriately repressing sets of neuronal genes in non-neuronal cells. (sciencedaily.com)
  • The role of exosomes collected by ultracentrifugation were also analyzed as mediators of p53 expression in cancer cells and APJ expression in fibroblasts. (spandidos-publications.com)
  • APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
  • In addition, xenografts of HCT116 cells with APJ‑suppressed fibroblasts showed accelerated tumor growth. (spandidos-publications.com)
  • MicroRNA 5703 enriched in exosomes derived from HCT116 sh p53 cells inhibited APJ expression, and inhibition of miR‑5703 diminished APJ suppression in fibroblasts caused by cancer cells. (spandidos-publications.com)
  • The mutational inactivation of the TP53 gene in tumor cells has been reported to affect not only tumor cells but also the surrounding cells in the tumor microenvironment and to promote tumor-stromal activation and subsequent tumor growth ( 11 , 12 ). (spandidos-publications.com)
  • The cells' gene expression signatures were tested for association with clinical outcome of breast cancer patients using survival data from The Cancer Genome Atlas database. (lu.se)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • These primary myelofibrosis progeny cells stimulate bone marrow fibroblasts (which are not part of the neoplastic transformation) to secrete excessive collagen. (msdmanuals.com)
  • The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
  • Ascorbic acid (AA) is essential for collagen biosynthesis as a cofactor for prolyl and lysyl hydroxylase and as a stimulus for collagen gene expression. (nih.gov)
  • Moreover, we determined the effects on type 1 and type 4 collagen and sodium-dependent vitamin C transporter (SVCT) gene expression when medium containing 100 μM AA was replaced every 24h for 5 days to avoid depletion of AA. (nih.gov)
  • Objective: For further investigation of the effect of bFGF on extracellular matrix homeostasis in the skin,we evaluated the expression of type I and type VII collagen gene at the transcriptional levels. (dsmc.or.kr)
  • Results: The steady state levels of type I and VII collagen gene mRNA were decreased with age dependent pattern up to 0.13 and 0.44 folds respectively. (dsmc.or.kr)
  • But there were no synergistic effects bFGF and TNP-α on type I collagen gene expression. (dsmc.or.kr)
  • The TNF-α showed slightly antagnostic effects on type VII collagen gene expression. (dsmc.or.kr)
  • The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. (nih.gov)
  • Surprisingly, the proliferation rate of the fibroblasts was increased in the presence of dexamethasone under conditions similar to those which caused slight decrease in the EGF receptor mRNA levels. (medicaljournals.se)
  • The DNA sequence of this gene is identical to the sequence of its mRNA and is devoid of introns. (elsevierpure.com)
  • DNA methylation regulates gene manifestation, so that it was also appealing to explore mRNA manifestation profiles A 839977 in the three cell types. (mdm-inhibitors.com)
  • Background: MicroRNAs (miRNAs) are endogenous, non-coding short RNAs (~22 nt) that can downregulate gene expression by translational repression, mRNA degradation, or transcriptional repression. (ac.ir)
  • Mutations in the genes encoding FGFRs, types 1-3, are responsible for various skeletal dysplasias and craniosynostosis syndromes. (nih.gov)
  • Mutations in the LMNA gene , encoding Lamin A /C (LMNA), are established causes of dilated cardiomyopathy (DCM). (bvsalud.org)
  • At least seven mutations in the FGF8 gene have been identified in people with Kallmann syndrome, a disorder characterized by the combination of hypogonadotropic hypogonadism (a condition affecting the production of hormones that direct sexual development) and an impaired sense of smell. (medlineplus.gov)
  • Researchers estimate that mutations in the FGF8 gene account for a small percentage of all cases of Kallmann syndrome. (medlineplus.gov)
  • Some people with Kallmann syndrome resulting from FGF8 gene mutations have additional features, such as a split in the lip (cleft lip) with an opening in the roof of the mouth (a cleft palate), and a condition called bimanual synkinesis, in which the movements of one hand are mirrored by the other hand. (medlineplus.gov)
  • It is unclear how mutations in the FGF8 gene lead to these other signs and symptoms. (medlineplus.gov)
  • Some affected individuals have mutations in one of several other genes in addition to FGF8 , and these genetic changes may contribute to the varied features of the condition. (medlineplus.gov)
  • Several mutations in the FGF8 gene have been found to cause a form of hypogonadotropic hypogonadism that occurs without an impaired sense of smell. (medlineplus.gov)
  • To date, more than 70 different FALDH gene mutations have been found in patients with Sjögren-Larsson syndrome. (medscape.com)
  • This syndrome is a result of mutations in the gene that codes for fatty aldehyde dehydrogenase. (medscape.com)
  • TP53 is a significant tumor suppressor gene, and its somatic mutations are one of the most frequent alterations in ~50% of all human cancers, including colorectal cancer ( 10 ). (spandidos-publications.com)
  • Mutations of the Janus kinase 2 ( JAK2 ) gene are present in a high proportion of cases of primary myelofibrosis. (msdmanuals.com)
  • Mutations of the thrombopoietin receptor gene ( MPL ) or the calreticulin ( CALR ) gene also may be the cause of primary myelofibrosis. (msdmanuals.com)
  • Taken together, our study demonstrated that GO-AgNPs potentially induce oxidative damage to DNA, which result in toxicity and cell apoptosis in caprine fetal fibroblast cell due to an increased generation of ROS. (researchsquare.com)
  • In each category, studies related to growth control genes, cell proliferation, apoptosis, DNA repair genes, antioxidant and drug detoxification genes, genes of drug metabolizing enzymes, tissue remodeling genes and genes of antibody-dependent cellular cytotoxicity were discussed separately. (researchsquare.com)
  • TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
  • The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
  • Despite the well-known difficulty in converting adult fibroblasts into functional neurons in vitro, we could detect functional maturation in the induced neurons. (lu.se)
  • Here we show that in young proliferating embryonic fibroblasts (MEFs) the Polycomb Repressive Complex 2 (PRC2) member EZH2 together with PRC1 members BMI1 and M33 are strongly expressed and localized at the INK4/ARF regulatory domain (RD) identified as a DNA replication origin. (plos.org)
  • In the absence of Bmi1, M33, or Phc2, primary embryonic fibroblasts (MEFs) are unable to progress into S phase, undergo premature senescence after only a few passages in culture and show an increased accumulation of the tumor suppressors p16 INK4a , p19 ARF and p15 INK4b [4] , [10] . (plos.org)
  • Fibroblast growth factors and fibroblast growth factor receptors (FGFRs) play important roles in human axial and craniofacial skeletal development. (nih.gov)
  • We investigated the extent to which fibroblasts isolated from diverse tissues differ in their capacity to modulate inflammation by comparing the global gene expression profiles of cultured human fibroblasts from skin, acute and chronically inflamed synovium, lymph node and tonsil. (birmingham.ac.uk)
  • After irradiation, signaling pathways rapidly modulate gene expression, which leads to additional signaling in the cell population both as a response to the initial damage and to maintain tissue homeostasis while the damage is being repaired [ 12 ]. (biomedcentral.com)
  • In order to investigate microRNA functions in gene regulation and/or to modulate their expression in pathogenic conditions, microRNAs are overexpressed by using plasmid or synthetic RNA duplexes designed to mimic the miRNA of interest. (ac.ir)
  • A pseudogene located on chromosome 1 has been found for this gene. (nih.gov)
  • The gene defect is located on chromosome 8, characterized as the intestinal zinc transporter gene, or SLC39A4 gene, at locus 8q24.3. (medscape.com)
  • Scholars@Duke publication: Tumour suppressor genes and angiogenesis: the role of TP53 in fibroblasts. (duke.edu)
  • RNA sequencing of LMNA-deficient and wild-type cardiac fibroblasts identified differential expression of ~410 genes , which predicted activation of the TP53 and TNFA/NFκB and suppression of the cell cycle pathways. (bvsalud.org)
  • Germline mutation of one TP53 allele is found in patients with Li-Fraumeni syndrome who generally inherit a mutated TP53 gene from an affected parent. (medscape.com)
  • Background: bFGF, a member of the fibroblast growth factor family, potently induces vascular smooth muscle cell proliferation and decreased synthesis of the collagens. (dsmc.or.kr)
  • It was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase . (wikidoc.org)
  • Little cell proliferation was observed for fibroblasts from CP and DGH as compared to NG. (bvsalud.org)
  • The expression of ALP is increased in CP and DGH, and fibroblasts in CP and DGH show low proliferation. (bvsalud.org)
  • Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
  • Here we illustrate the ability of human CMV (HCMV) to stimulate cellular thymidylate synthase (TS) gene expression in quiescent human embryonic lung fibroblasts. (elsevierpure.com)
  • Amphiregulin modulates murine lung recovery and fibroblast function following exposure to agricultur e organic dust. (cdc.gov)
  • In murine and human lung fibroblasts, DE induced the release of AREG and inflammatory cytokines. (cdc.gov)
  • Fibroblast recellularization of primary human lung mesenchymal matrix scaffolds and wound closure was inhibited by DE and enhanced with recombinant AREG alone. (cdc.gov)
  • Senior author Ramin Shiekhattar is a professor in two programs at Wistar, the gene expression and regulation program and molecular and cellular oncogenesis program. (sciencedaily.com)
  • Regulation and function of Zip4, the acrodermatitis enteropathica gene. (medscape.com)
  • CONCLUSIONS: Seven genes have been identified with differential expression patterns in terms of disease process (osteoarthritis vs rheumatoid arthritis), state of activation (resting vs cytokine activation) and anatomical location (synovium vs skin). (ox.ac.uk)
  • A total of 7 possible genetic alterations and epigenetic changes in CAFs are discussed, including gene differential expression, karyotype analysis, gene copy number variation, loss of heterozygosis, allelic imbalance, microsatellite instability, post‑transcriptional control and DNA methylation. (spandidos-publications.com)
  • The present review summarizes the recent achievements in understanding the gene profiles of CAFs and pays special attention to their possible epigenetic alterations. (spandidos-publications.com)
  • CAFs are different from NFs, and their unique phenotypes and functions are partly determined by differences in gene expression. (spandidos-publications.com)
  • in one study, 31 genes in breast CAFs, which were identified using Affymetrix Human Genome U133 Plus 2.0 and an empirical Bayesian model, were different from those in NFs ( 11 ). (spandidos-publications.com)
  • Genes mainly involved in coding adhesion molecules and growth factors have also been found to be upregulated in other types of CAFs, including colon ( 12 ) and pancreatic ( 13 ). (spandidos-publications.com)
  • These results are consistent with another study concerning the gene expression profiling of breast CAFs, which were detected using a complementary DNA microarray ( 15 ). (spandidos-publications.com)
  • To address this, we here established two autologous CAF lines representing inflammatory CAFs (iCAFs) and myofibroblast CAFs (myCAFs) and compared them with already established interlobular- and lobular fibroblasts with respect to their origin and impact on tumor formation. (lu.se)
  • GO-AgNPs induced significant cytotoxicity by the loss of cell viability, production of reactive oxygen species (ROS), cell cycle arrest, increasing leakage of lactate dehydrogenase (LDH) and level of Malondialdehyde (MDA), increasing expression of pro-apoptotic genes and decreasing expression of anti-apoptotic genes. (researchsquare.com)
  • We generated immortalized wild-type (WT) and menin-null mouse embryo fibroblast (MEF) cell lines and evaluated their characteristics, including global expression patterns. (johnshopkins.edu)
  • However, LMNA is also expressed in other cardiac cell types, including fibroblasts . (bvsalud.org)
  • Extremely lethal consequences of IR causes double-strand breaks, which are mainly responsible for genomic instability, altered gene expression, and cell death. (biomedcentral.com)
  • Just five gene loci were methylated in every 3 cell types differentially. (mdm-inhibitors.com)
  • The ultimate goal of this research was to recognize which cell features would be suffering from schizophrenia-associated variations in DNA methylation and gene manifestation. (mdm-inhibitors.com)
  • Nevertheless, the generation of neurons from adult human fibroblasts, an easily accessible cell source to obtain patient-derived neurons, has proved to be challenging due to the intrinsic blockade of neuronal commitment. (lu.se)
  • Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)
  • and fibroblast-epithelial cell coculture, the latter coupled with fluorescence-activated cell sorting. (nih.gov)
  • Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • APJ suppression from a specific microRNA in cancer cell‑derived exosomes induced CAF‑like properties in fibroblasts. (spandidos-publications.com)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • Using an enzymatic digestion procedure, gingival cell suspensions containing ALP-positive fibroblasts were prepared without affecting ALP activity. (bvsalud.org)
  • Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
  • Replication may be categorized into immediate early, delayed early, and late gene expression based on time of synthesis after infection. (medscape.com)
  • Fibrostenotic eosinophilic esophagitis might reflect epithelial lysyl oxidase induction by fibroblast-derived TNF-α. (nih.gov)
  • We find that fibroblast-derived TNF-α stimulates epithelial LOX expression through activation of nuclear factor κB and TGF-β-mediated signaling. (nih.gov)
  • Transient transfection of promoter-chloramphenicol O-acetyltransferase reporter constructs into primary human connective tissue fibroblasts shows that a 904 bp fragment that hybridizes to a murine TIMP-1 promoter fragment contains a functional promoter. (uea.ac.uk)
  • Conclusion: Unlike miR-expressing vector or synthetic RNA duplexes designed to mimic the miRNA of interest that can downregulate gene expression, synthetic 22 nucleotide mature single stranded microRNA probably induces gene expression in a locus-specific manner. (ac.ir)
  • Deletion of the Lmna gene in fibroblasts causes senescence-associated dilated cardiomyopathy by activating the double-stranded DNA damage response and induction of senescence-associated secretory phenotype. (bvsalud.org)
  • These results and further mapping with 5' deletion mutants from the -738/+95 region have demonstrated that an AP-1 site at -92/-86 is essential for basal expression of the gene. (uea.ac.uk)
  • Fibroblast Growth Factor 2 Levels in Patients with Major Depressive Disorder: A Meta-analysis. (nih.gov)
  • Autosomal dominant disorders of skeletal and cranial development have been linked to fibroblast growth factor receptor ( FGFR) 2 and FGFR3 . (aacrjournals.org)
  • FGFR 3 encodes a transmembrane tyrosine kinase receptor involved in signaling via interaction with the family of fibroblast growth factors. (aacrjournals.org)
  • Database searches and analysis of available genomic DNA sequence from the region revealed the presence of the fibroblast growth factor homologous factor gene, FHF2, within the duplication breakpoint interval. (edu.au)
  • Specifically, transcripts for fibulin 2 (Fbln2), periostin (Postn), and versican [chondroitin sulfate proteoglycan (Cspg2)], genes critical for the developing heart and known to be induced by transforming growth factor-β (TGF-β), were decreased in their expression in menin-null MEFs. (johnshopkins.edu)
  • The Lmna gene was deleted by crossing the platelet-derived growth factor receptor α-Cre recombinase (Pdgfra-Cre) and floxed Lmna (Lmna F/F) mice . (bvsalud.org)
  • Fusions of fibroblast growth factor receptor genes with other partner genes were recently discovered in cholangiocarcinoma. (mayo.edu)
  • The Hepatobiliary Cancer Lab is identifying and characterizing fibroblast growth factor receptor family gene aberrations and determining the effectiveness of targeting the aberrations in the treatment of cholangiocarcinoma. (mayo.edu)
  • Drug resistance to fibroblast growth factor receptor inhibitors (FGFR) has emerged as a major challenge to their clinical use. (mayo.edu)
  • Inference of origin in interlobular and lobular fibroblasts relied on RNA-Seq profiles, immunocytochemistry and growth characteristics. (lu.se)
  • Affected individuals carry a germ-line loss-of-function mutation of the MEN1 gene, and tumors arise after loss of the second allele. (johnshopkins.edu)
  • FAPI-04 represents a promising tracer for both diagnostic imaging and, possibly, targeted therapy of malignant tumors with a high content of activated fibroblasts, such as breast cancer. (snmjournals.org)
  • Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)
  • In order to solve this problem, a comprehensive network analysis method was established based-on "component-gene ontology-effect" interactions. (frontiersin.org)
  • In conclusion, the three-mode gene ontology network can be an effective network analysis workflow to evaluate the pharmacological effects of a multi-drug complex system. (frontiersin.org)
  • For the construction of a "component-gene ontology" network of Schisandra in the treatment of AMI, the HPLC-Q-TOF-MS was used to analyze the Schisandra extract. (frontiersin.org)
  • Targets of Schisandra ingredients were further screened to enrich and construct the "component-gene ontology" network, which was a component for the integration of a three-mode network. (frontiersin.org)
  • RESULTS: Gene ontology and pathway analyses linked TNF-α and LOX expression in patients with EoE, which was validated in independent sets of patients with fibrostenotic conditions. (nih.gov)
  • A recombinant λ bacteriophage isolated from a human genome library contains the gene for fibroblast interferon (IFN-β1). (elsevierpure.com)
  • Viruses induce interferon-stimulated genes but they also regulate the expression of many hundreds of cellular metabolic and structural genes to facilitate their replication. (essex.ac.uk)
  • 80%) and biological pathways (such as adipocytokine, Notch, Hedgehog and NOD-like receptor signaling) were not identified by previous gene array studies. (mdpi.com)
  • The goal of our search was to identify publications describing the role of specific genes, gene signatures, or molecular pathways and their effects in response to environmental exposures. (cdc.gov)
  • Genetic alterations include genetic mutation, gene copy number variation (CNV), loss of heterozygosity (LOH), allelic imbalance (AI) and microsatellite instability (MSI). (spandidos-publications.com)
  • Sjögren-Larsson syndrome (SLS) is diagnosed by demonstrating the enzyme deficiency or by mutation analysis of the FALDH gene. (medscape.com)
  • The AE mutation creates reduced zinc uptake and abnormal zinc metabolism in human fibroblasts. (medscape.com)
  • Our data supports the concept that fibroblasts are heterogeneous, and that they contribute to the tissue-specificity of inflammatory reactions. (birmingham.ac.uk)
  • Collectively, these studies demonstrate that inhibition of AREG reduced, whereas AREG supplementation promoted, the airway inflammatory recovery response following environmental bioaerosol exposure, and AREG enhanced fibroblast function, suggesting that AREG could be targeted in agricultur al workers repetitively exposed to organic dust environments to potentially prevent and/ or reduce disease. (cdc.gov)
  • This suggests that periodontal inflammation and diphenylhydantoin may influence ALP expression and human gingival fibroblast expansion. (bvsalud.org)
  • Simultaneous treatment of the fibroblasts with 13-cis-RA and dexamethasone resulted in similar decreases in EGF receptor and PG40 mRNAs as with 13-cis-RA alone. (medicaljournals.se)
  • The apelin‑apelin receptor (APJ) system has been implicated in suppressing fibroblast‑to‑myofibroblast transition in non‑neoplastic organ fibrosis. (spandidos-publications.com)
  • Fibroblasts are therefore likely to play an active role in the persistence of chronic inflammatory reactions. (birmingham.ac.uk)
  • This proved to be biologically relevant, as TNF-alpha induced the secretion of different patterns and amounts of IL-6, IL-8 and CCL2 (MCP-1) in the two fibroblast types. (birmingham.ac.uk)
  • Functionally, myCAFs and lobular fibroblasts are almost equally tumor promoting as opposed to iCAFs and interlobular fibroblasts. (lu.se)
  • In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1. (lu.se)
  • The gene structure of FHF2 was determined and two new exons were identified, including a new 5′ end exon, 1B. (edu.au)
  • FHF2 is a large gene extending over ∼200 kb in Xq26.3 and is composed of at least seven exons. (edu.au)
  • AREG treatment rescued the DE-induced inhibitory fibroblast effects. (cdc.gov)
  • In addition, these alterations affect 3 principal categories of genes, as follows: proto-oncogenes, tumor suppressor genes, and DNA repair genes. (medscape.com)
  • The increase in TS gene expression was due to an increase in gene transcription, since the expression of a reporter gene driven by the human TS promoter was strongly induced by HCMV infection. (elsevierpure.com)
  • Our data suggest that HCMV activates human TS gene transcription by mechanisms that independent of E2F and different from those used by MCMV to stimulate the mouse TS promoter. (elsevierpure.com)
  • The findings implicate epigenetic control of gene expression in addition to transcription factor networks. (biomedcentral.com)
  • Reverse transcription quantitative polymerase chain reaction and Western blot analyses further confirmed that the expression of osteogenic-specific genes and major matrix components were up-regulated. (researchgate.net)
  • Understanding the interactions between our environment and our genes and how they affect health outcomes offers a multitude of potential opportunities in public health. (cdc.gov)
  • Finally, expression of pH2AFX, a bonafide marker of the double-stranded DNA breaks , was increased in cardiac fibroblasts isolated from the Pdgfra-CreLmna F/F mouse hearts . (bvsalud.org)