AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Citrobacter freundiiCitrobacterCitrobacter rodentiumEnterobacteriaceae InfectionsTyrosine Phenol-LyaseEscherichiaCitrobacter koseriEnterobacteriaceaeCephalosporinasebeta-LactamasesEnterobacterEnterobacter cloacaeMicrobial Sensitivity TestsCefotaximeDicloxacillinPyronineSerratiaGram-Negative BacteriaProteus vulgarisCephalosporinsUranium CompoundsAnti-Bacterial AgentsEscherichia coliBacteriaCeftazidimeKlebsiella oxytocaSerratia marcescensCefotiamBacterial ProteinsGenes, BacterialCefmenoximeMonobactamsCephaloridinebeta-LactamsDrug Resistance, MicrobialPlasmidsPenicillinaseCephalosporin ResistanceKlebsiellaCloxacillinMolecular Sequence Databeta-Lactam ResistanceThienamycinsGram-Positive BacteriaDNA, BacterialConjugation, GeneticCefiximeProteus mirabilisCarbapenemsAmpicillinKlebsiella pneumoniaeProteusPenicillanic AcidSalmonellaCeftizoximeBase SequenceIsoelectric FocusingEscherichia coli ProteinsChromosomes, BacterialImipenem
EnterobacterKlebsiellaEscherichiaPlasmidKoseriIsolatesSerratiaCarbapenemaseAcinetobacterNucleotide sequenceBacteriaResistance genesStuartiiESBLSpeciesCharacterizationClinicalProteusCarbapenemasesAntibiotic-resistanceQuinoloneGenomicOxytocaPneumoniaeChromosomeBlaKPCDetectionESBLsFrequencyGenomeDetectResistantComplexExpressionPatients
Enterobacter6
Klebsiella4
  • ABSTRACT The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the bla gene that encodes for carbapenemase production have complicated the management of patient infections. (who.int)
  • Molecular Analysis of bla(KPC-2)-Harboring Plasmids: Tn4401a Interplasmid Transposition and Tn4401a-Carrying ColRNAI Plasmid Mobilization from Klebsiella pneumoniae to Citrobacter europaeus and Morganella morganii in a Single Patient. (helmholtz-hzi.de)
  • Survey and rapid detection of Klebsiella pneumoniae in clinical samples targeting the rcsA gene in Beijing, China. (omicsdi.org)
  • Strikingly, of the 32 K. pneumoniae strains, 18 contained the Klebsiella pneumoniae Carbapenemase (KPC)-encoding gene bla KPC-2 and had high resistance to β-lactam antibiotics. (omicsdi.org)
Escherichia2
  • 5. Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. (ac.ir)
  • Phylum barrier and Escherichia coli intra-species phylogeny drive the acquisition of antibiotic-resistance genes. (iame-research.center)
Plasmid4
  • The mobilizable IncP-6 plasmid pLNU-11 was discovered in a strain of Citrobacter freundii enriched from the wetland sediments with tetracycline and nalidixic acid, and encodes a novel AmpC-like ß-lactamase (blaWDC-1), which shares less than 62% amino acid sequence identity with the PDC class of ß-lactamases found in Pseudomonas aeruginosa. (pacb.com)
  • KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)
  • KPC-2 was the predominantly detected carbapenemase (n=19) and was located in 95% of cases on a highly-conserved multiple-drug-resistance-gene-carrying pMLST15 IncN plasmid. (helmholtz-hzi.de)
  • The complete and annotated nucleotide sequence of a 54,036-bp plasmid harboring a blaKPC-2 gene that is clonally present in Citrobacter isolates from different species is presented. (uni-giessen.de)
Koseri1
  • C. freundii, C. portucalensis, C. europaeus, C. koseri and C. braakii) were increasingly detected (n=56) within the study period. (helmholtz-hzi.de)
Isolates7
  • High resistance to most of the antibiotics tested was observed among the isolates, five ST25, three ST11, one ST45, and one ST505, which harbored a total of 44 plasmids, many of them predicted to be conjugative and carrying genes conferring resistance to a variety of antibiotic, metals, and disinfectants. (biorxiv.org)
  • This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the bla gene and compared the results with modified Hodge test. (who.int)
  • The two CR isolates of E. coli possessed blaKPC and blaOXA-48 genes. (bvsalud.org)
  • We analyzed the genomes of 512 isolates of 21 CRE species obtained from 61 hospitals within a three-year-period and found that Citrobacter spp. (helmholtz-hzi.de)
  • Lastly, DNA was extracted from the isolates to confirm the presence of bla genes that encode for ESBLs by agarose gel electrophoresis. (ung.edu)
  • The blaKPC gene was confirmed in 14/20 (70.0%) of these isolates. (who.int)
  • Ceftazidime-resistant (CAZ res ) isolates with a positive double-disc synergy test were screened for the presence of extended-spectrum β-lactamase-encoding genes. (advbiores.net)
Serratia1
Carbapenemase3
Acinetobacter1
  • Strain S2-1) , P. stutzeri (Strain R3-2) , P. chloritidismutans (Strain K4-1) , Achromobacter xylosoxidans (Strain K4-3) , Acinetobacter radioresistens (Strain S1-2) , Acinetobacter calcoaceticus (Strain K4-2) and Citrobacter freundii (Strain R3-1). (ac.ir)
Nucleotide sequence1
  • SRST2 uses nucleotide sequence to assign gene alleles from sequencing reads. (cdc.gov)
Bacteria2
  • To test this hypothesis, the phylogenetic composition of faecal microbiomes was investigated by a 16S rRNA gene amplicon sequence-based approach for Bacteria and Archaea . (springer.com)
  • Tigecycline is a new-generation of tetracycline (glycylcyclines) and is active in vitro against bacteria that possess any of the classical genes that confer tetracycline resistance through ribosomal protection or efflux pumps. (druglib.com)
Resistance genes3
  • Multidrug and carbapenem-resistant K. pneumoniae (CR- Kp ) are considered critical threats to global health and key traffickers of resistance genes to other pathogens. (biorxiv.org)
  • The plasmids ranged in size from 36 to 144 kb and encoded known resistance genes for most of the major classes of antibiotics used to treat Gram-negative infections (tetracyclines, sulfonamides, ß-lactams, fluoroquinolones, aminoglycosides, and amphenicols). (pacb.com)
  • ResFinderFG v2.0: a database of antibiotic resistance genes obtained by functional metagenomics. (iame-research.center)
Stuartii1
  • Dessa forma, este estudo demonstrou a disseminação do gene blaKPC-2 em 9 Enterobacteriaceae de diferentes regiões do Brasil, incluindo espécies nas quais o gene não havia sido previamente descrito, como P. agglomerans e P. Stuartii. (fiocruz.br)
ESBL2
  • The bla genes that code for ESBL can be passed via horizontal transmission. (ung.edu)
  • Since ESBL genes are transmissible, it is important that ESBLs be tested for in other organisms in hospital and long-term care facility patient populations where ESBLs are encountered. (asm.org)
Species2
  • These results underline the increasing importance of the Citrobacter species as emerging carriers of carbapenemases and therefore as potential disseminators of Carbapenem- and multidrug-resistance in the hospital setting. (helmholtz-hzi.de)
  • Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a α/β, ATP-binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. (utmb.edu)
Characterization1
Clinical3
  • This study investigates the contribution of efflux mechanism to carbapenem resistance in 61 acquired blaCHDL-genes-carrying A. baumannii clinical strains isolated in Warsaw, Poland. (bvsalud.org)
  • In this study, we first described a loop-mediated isothermal amplification (LAMP) method for the rapid detection of capsular polysaccharide synthesis regulating gene rcsA from K. pneumoniaein clinical samples by using two methods including real-time turbidity monitoring and fluorescence detection to assess the reaction. (omicsdi.org)
  • Our data showed the high prevalence of bla KPC-2 among K. pneumoniae and co-occurrence of many resistant genes in the clinical strains signal a rapid and continuing evolution of K. pneumoniae. (omicsdi.org)
Proteus1
Carbapenemases2
  • Ten plasmids encoding either KPC-2, NDM-1, or NDM-7 carbapenemases were characterized, including novel plasmids with increased resistance gene load and a novel genetic environment for bla KPC-2 gene. (biorxiv.org)
  • Multiplex PCR was used for detection of genes encoding carbapenemases from different classes (blaKPC, blaIMP, blaVIM, blaNDM, blaOXA-48). (bvsalud.org)
Antibiotic-resistance1
  • This analysis does not include mutations that may result in antibiotic resistance or resistance determinants added to newer versions of used database or other antimicrobial resistance gene databases. (cdc.gov)
Quinolone1
  • The aim of this study was the frequency assay of qnr plasmid's genes in quinolone resistant Entrobacteriaceae strains isolated from urban and hospital wastewaters. (wwjournal.ir)
Genomic1
  • The high genomic diversity of C. freundii is represented by 22 ST-types. (helmholtz-hzi.de)
Oxytoca1
  • As espécies mais resistentes foram E. aerogenes, E. cloacae, C. freundii e K. oxytoca. (fiocruz.br)
Pneumoniae2
  • KPC prévalence de la non sensibilité à l'ertapénème dans un hôpital de soins tertiaires en Égypte était principalement imputable aux mécanismes de résistance médiés par les carbapénèmases dans les isolats de K. pneumoniae . (who.int)
  • La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)
Chromosome2
  • Altered gene is located on this chromosome. (edu.au)
  • Of the genes that lie within chromosome area defined by the microsatellite markers the most likely genes to be involved with this strain are. (edu.au)
BlaKPC5
  • Contudo, pouco se sabe sobre a epidemiologia da disseminação do gene blaKPC-2 nestas outras espécies. (fiocruz.br)
  • A variante alélica de blaKPC, assim como a participação do transposon Tn4401 e a análise da presença de outros genes de beta-lactamases (TEM, SHV e CTX-M) foram realizadas por PCR e sequenciamento. (fiocruz.br)
  • Análise plasmidial e hibridação foram realizadas para determinar o ambiente genético do gene blaKPC. (fiocruz.br)
  • A associação de KPC com outras beta-lactamases foi encontrada em 53% das amostras (45,8% produtoras de TEM, 6% produtoras de SHV e 22,9% produtoras de CTX-M). O gene blaKPC-2 foi encontrado associado a uma estrutura parcial do transposon Tn4401 com diferentes isoformas. (fiocruz.br)
  • Todas as amostras apresentaram o gene blaKPC-2 inserido em plasmídios de vários tamanhos e grupos de incompatibilidade Observamos grande diversidade genética entre todas as espécies estudadas, mas encontramos, para algumas espécies, a presença de alguns grupos clonais prevalentes em determinados estados. (fiocruz.br)
Detection1
  • Sensitive and rapid detection of the new Delhi metallo-beta-lactamase gene by loop-mediated isothermal amplification. (omicsdi.org)
ESBLs2
  • The earliest variants of ESBLs originated as a result of point mutations in the genes for broad-spectrum β-lactamases whilst newer ones, including the most successful such as CTX-M, arose by acquisition from the environmental metagenome through horizontal gene transfer. (ajlmonline.org)
  • ESBLs are typically inhibitor-susceptible β-lactamases that hydrolyze penicillins, cephalosporins, and aztreonam and are encoded by mobile genes. (asm.org)
Frequency1
  • A number of resistance mechanisms are responsible for the emergence and prevalence of antimicrobial resistance, and such mechanisms have been divided into genetic mutation occurred at a low frequency and acquisition of various genes mediated resistance to their host microorganisms. (biomedcentral.com)
Genome2
  • Studies were conducted using phenotypic (susceptibility testing to carbapenems ± efflux pump inhibitors (EPIs)) and molecular (determining expression levels of efflux operon with regulatory-gene and whole genome sequencing (WGS)) methods. (bvsalud.org)
  • The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. (utmb.edu)
Detect1
  • This quantitative approach uses TaqMan chemistry from Life Technologies to detect pathogen-specific gene sequences. (retrogen.net)
Resistant1
Complex1
Expression1
  • Contrasting patterns of gene expression indicate differing pyrethroid resistance mechanisms across the range of the New World malaria vector Anopheles albimanus. (cdc.gov)
Patients1
  • Methylenetetrahydrofolate reductase gene polymorphisms in Egyptian Turner Syndrome patients. (edu.pl)