• mRNA
  • This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. (mybiosource.com)
  • Coronavirus replication is known to affect cellular translation, involving activation of stress-induced signaling pathways, and employing viral proteins that affect cellular mRNA translation and RNA stability. (pubmedcentralcanada.ca)
  • The translational initiation step includes the recognition of an mRNA by the host translational machinery and assembly of the 80S complex, in which a methionyl initiator tRNA (Met-tRNA Met ) binds at the peptidyl (P) site of the mRNA. (pubmedcentralcanada.ca)
  • Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl-heidelberg.de)
  • 40S Ribosomal Subunits scanning the mRNA from 5' have TC bound and translate the first upstream open reading frame (uORF). (wikipedia.org)
  • Translation is again initiated, uORF2,3 or 4 translated and the 40S Subunits subsequently dissociate from GCN4 mRNA. (wikipedia.org)
  • By using a tyrosine hydroxylase antibody, we found that the dopaminergic neurons of the substantia nigra and the noradrenergic neurons of the locus ceruleus express mRNA for the alpha subunits for each of Gi, Go, and Gs. (ubc.ca)
  • High levels Golf mRNA and protein were detected in the striatum and nucleus accumbens, in addition to the expected high levels in the olfactory tubercle. (ubc.ca)
  • novel
  • Discovery of these novel GAP, GDI, and GEF activities have helped to illuminate a new role for Gα subunit GDP/GTP cycling required for microtubule force generation and mitotic spindle function in chromosomal segregation. (ijbs.com)
  • We have identified the novel Galphaz-binding protein, which is referred to as the G-protein-regulated inducer of neurite outgrowth (GRIN1) using the far-western method. (genes2cognition.org)
  • intracellular
  • Signaling molecules bind to a domain of the GPCR located outside the cell, and an intracellular GPCR domain then in turn activates a particular G protein. (wikipedia.org)
  • Being obligate intracellular parasites, viruses heavily depend on host cell structures and functions to complete their life cycle, and they also use the translational apparatus of the infected cell to express their proteins. (pubmedcentralcanada.ca)
  • ribosomal
  • Translational initiation requires at least nine eukaryotic initiation factors (eIFs) and comprises two steps: the formation of 48S initiation complexes with established codon-anticodon base pairing in the P-site of the 40S ribosomal subunits, and the joining of 60S subunits to 48S complexes to form the 80S complex. (pubmedcentralcanada.ca)
  • interaction
  • Coexpression of RGS11 with different Gbeta subunits reveals specific interaction between RGS11 and Gbeta5. (embl-heidelberg.de)
  • The Gγ subunit is considerably smaller than Gβ, and is unstable on its own, requiring interaction with Gβ to fold, explaining the close association of the dimer. (wikipedia.org)
  • This prenylation is thought to be involved in directing the interaction of the subunit both with membrane lipids and other proteins. (wikipedia.org)
  • polypeptide
  • Folding of the β-subunit is thought to be aided by the chaperone CCT (chaperonin containing tailless-complex polypeptide 1), which also prevents aggregation of folded subunits. (wikipedia.org)
  • 1. A nucleic acid encoding a mutated Gq protein identical to the polypeptide contained in SEQ ID NO:1 or 37 except for the substitution of an aspartic acid residue for theglycine residue at position 66. (patentgenius.com)
  • 6. A nucleic acid encoding a mutated Gq protein which is identical to the polypeptide contained in SEQ ID NO:1 except for the replacement of the last 5 amino acid residues with the 5 amino acid residues of the carboxy terminus of Golf ortransducin. (patentgenius.com)
  • specificity
  • The Gβ genes have very similar sequences, while significantly greater variation is seen in the Gγ genes, indicating that the functional specificity of the Gβγ dimer may be dependent on the type of Gγ subunit involved. (wikipedia.org)
  • membrane
  • This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. (wikipedia.org)
  • The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. (abcam.com)
  • Finally, PhLP is released, exposing the binding site for Gα, allowing for formation of the final trimer at the endoplasmic reticulum, where it is targeted to the plasma membrane. (wikipedia.org)
  • The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. (wikipedia.org)
  • tRNA
  • In elongation, aminoacyl tRNAs enter the acceptor (A) site and, if the correct tRNA is bound, the ribosome catalyzes the formation of a peptide bond. (pubmedcentralcanada.ca)
  • Binding of uncharged tRNA to this synthetase-like domain induces a conformational change in which the GCN2 domains rotate 180° normal to the dimerization surface and thereby transpose from their antiparallel to a parallel orientation. (wikipedia.org)
  • amino
  • Mutagenesis of the amino terminus of the alpha subunit of the G protein Go. (wikipedia.org)
  • This protein contains a conserved, 120 amino acid motif called the RGS domain and a cysteine-rich region. (wikipedia.org)
  • The non-WD-repeat amino terminal alpha helix of G beta does not inhibit folding because G beta does not fold even when this region is removed. (embl-heidelberg.de)
  • Elevated levels of GCN4 stimulate the expression of amino acid biosynthetic genes, which code for enzymes required to synthesize all 20 major amino acids. (wikipedia.org)
  • E coli -derived fusion protein as test antigen (amino acids 1-85 of human RGS8 + 52kDa in-frame fusion partner). (abcam.com)
  • regulate
  • Although these hypotheses were initially controversial, Gβγ has since been shown to directly regulate as many different protein targets as the Gα subunit. (wikipedia.org)
  • Complex
  • The Gs alpha subunit of the stimulated G protein complex exchanges GDP for GTP and is released from the complex. (wikipedia.org)
  • Upon activation of the GPCR, the Gβγ complex is released from the Gα subunit after its GDP-GTP exchange. (wikipedia.org)
  • The free Gβγ complex can act as a signaling molecule itself, by activating other second messengers or by gating ion channels directly. (wikipedia.org)
  • The individual subunits of the G protein complex were first identified in 1980 when the regulatory component of adenylate cyclase was successfully purified, yielding three polypeptides of different molecular weights. (wikipedia.org)
  • However, downstream signalling effects of Gβγ were later discovered when the purified Gβγ complex was found to activate a cardiac muscarinic K+ channel. (wikipedia.org)
  • It may associate with other G-protein-activated potassium channel subunits to form a heterotetrameric pore-forming complex. (wikipedia.org)
  • RNA polymerase II (RNAP II and Pol II) is a protein-protein complex. (wikipedia.org)
  • A 550 kDa complex of 12 subunits, RNAP II is the most studied type of RNA polymerase. (wikipedia.org)
  • Once RPB1 enters the complex, other subunits such as RPB5 and RPB7 can enter, where RPB5 binds to RPB6 and RPB8 and RPB3 brings in RPB10, RPB 11, and RPB12. (wikipedia.org)
  • ligand
  • Each GPCR binds to and is activated by a specific ligand stimulus that ranges in size from small molecule catecholamines, lipids, or neurotransmitters to large protein hormones. (wikipedia.org)
  • The protocol involves overexpression of the GFP-fused protein, cell lysis in non-denaturing conditions, and detection of MST signal in the presence of varying concentrations of the ligand. (jove.com)
  • mammalian
  • The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. (genes2cognition.org)
  • sub.q protein variants can be expressed in mammalian cell lines or Xenopus oocytes, and then evaluated using calcium fluorescence imaging and electrophysiological recording. (patentgenius.com)
  • eukaryotic
  • G protein signaling mediates the perception of environmental cues in all higher eukaryotic organisms, including yeast, Dictyostelium, plants, and animals. (patentgenius.com)