Gene open reading frames. Medical search. Frequent questions

Open reading frames (ORFs) predicted by at least two of the programs were amplified and spotted onto glass slides. (the-scientist.com)
We found 191 putative coding genes (ORFs) of which 72 genes were previously known, and 110 of which remain unidentified despite literature and similarity searches. (nih.gov)
The hepatitis E virus (HEV) genome contains three open reading frames (ORFs). (medscape.com)
It is an unenveloped, non-covalently closed, bacilliform double-stranded DNA (dsDNA) virus with a monopartite genome of ~7.2-7.8 kb encoding three open-reading frames (ORFs). (nature.com)
The male sterilizing factors are produced by recombination of the mitochondrial genome and consist of unusual genes or open reading frames (ORFs) that usually contain a portion of functional mitochondrial genes and sequences of unknown origin ( Hanson and Bentolila, 2004 ). (frontiersin.org)
DNA sequence analysis revealed that two major open reading frames (ORFs) are in the LR gene. (unl.edu)
325 open reading frames (ORFs) were compared with those of the recently sequenced genome of the Serva vaccine strain. (usda.gov)
The Center for Cancer Systems Biology of the Dana-Farber Institute created a collection of human open reading frames (ORFs) cloned into a Gateway-entry vector, known as the CCSB Human ORFeome Collection. (horizondiscovery.com)
These Gateway-adapted ORFs are ideal for easily moving gene content into compatible destination vectors for various proteomics studies. (horizondiscovery.com)
Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). (horizondiscovery.com)

The fact that the Gimap genes are located together in a tight cluster on RNO4 (and in conserved synteny with many other species), combined with their sequence similarities, suggests the possibility that the proteins carry out similar function. (hindawi.com)
In the majority of cases, Rf genes produce proteins that act directly on the CMS conferring mitochondrial transcripts by binding them specifically and promoting processing events. (frontiersin.org)
In the majority of cases, Rf genes produce proteins that bind specifically to the CMS conferring transcripts in the mitochondria and promote processing events leading to a strong reduction in the production of mitochondrial CMS-inducing proteins (reviewed in Chen and Liu, 2014 ). (frontiersin.org)
Exclusive to the genomes of the Serva vaccine strains, 7 non-synonymous mutation were identified in the predicted translation products of the genes encoding glycoproteins gB, gE, gL and gM and three nonstructural proteins UL28 (DNA packaging protein), UL5 (helicase-primase) and the immediate early protein ICP4. (usda.gov)
As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (ucsc.edu)
Genome annotation disclosed 29 SARS-CoV-2 gene products - including 16 non-structural proteins, 4 structural proteins and 9 accessory factors. (news-medical.net)
In short, from a total of 437 high-confidence interacting proteins that bind to one or more SARS-CoV-2 genes, the researchers have identified several gene products, M protein, NSP6, ORF3a, ORF6 and ORF7b that interacted with host cell membrane proteins and complexes. (news-medical.net)
The 16 non-structure proteins (NSPs) are cleaved products of the large polyprotein open reading frame (ORF)1ab or ORF1a. (news-medical.net)
Although some scientists expected to find at least 100,000 genes coding for proteins, only about 30,000-35,000 of such genes appear to be in the human genome. (learner.org)
They included genes coding for proteins with domains homologous to calcium transporters, casein kinase II, UBC13, AMSH, Vps23p, and Vps27p of Saccharomyces cerevisiae. (duke.edu)
Since many of these gene products are homologous to the proteins involved in vesicle transport, sterility caused by inactivation of ste12 may be due to a disordered vesicle transport system. (duke.edu)

At least five genes were shown to be essential for the synthesis of AHBA, five genes were determined to encode the modular type I PKS that uses AHBA as the starter unit, and 20 or more genes appear to govern modification of the polyketide-derived framework, and rifamycin resistance and export. (nih.gov)
Disruption of the PKS genes at the end of rifA abolished rifamycin B production and resulted in the formation of P8/1-OG, a known shunt product of rifamycin biosynthesis, whereas disruption of the orf6 and orf9 genes, which may encode deoxysugar biosynthesis enzymes, had no apparent effect. (nih.gov)
The mauB, mauA, and mauC genes encode the periplasmic methylamine dehydrogenase (MADH) large and small subunit polypeptides and amicyanin, respectively. (caltech.edu)
The genomes of Antiqua and Nepal516 are 4.7 Mb and 4.5 Mb and encode 4,138 and 3,956 open reading frames, respectively. (asm.org)

Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR. lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background. (hindawi.com)
We have cloned and sequenced cDNA of trypsin gene from Aedes (Ochlerotatus) taeniorhynchus (Weidemann) adult. (usda.gov)
More than 50 million high-quality reads from a cDNA library were generated and de novo assembled. (biomedcentral.com)
We tried several gene selection strategies, and built classifiers using the resulting cDNA microarray gene lists. (lu.se)

Putative regulatory genes were also identified. (nih.gov)

taeniorhynchus (AetTryp) was obtained which encodes an open reading frame of 717 bp (i.e., 239 aa). (usda.gov)
The M protein gene ( emm) encodes the cell surface M virulence protein responsible for at least 100 Streptococcus pyogenes M serotypes. (cdc.gov)
This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. (ucsc.edu)
KJ892031 - Synthetic construct Homo sapiens clone ccsbBroadEn_01425 RPL22 gene, encodes complete protein. (ucsc.edu)
This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. (ucsc.edu)

Within LR promoter sequences, a small open reading frame (ORF) was identified (ORF-E) that is antisense to the LR-RNA, and downstream of the bICP0 gene. (nebraska.edu)
One tRNA gene (proK) and two insertion sequences (IS5 and IS150) are located in this segment. (nih.gov)
These sequences are usually linked to M protein genes rather than the similar mrp or enn genes. (cdc.gov)
This helps to avoid confounding of the 180-base M protein gene segment by the similar emm- like mrp and enn sequences (see CDC Streptococcus Laboratory GAS bioinformatic pipeline for S. pyogenes external icon ). (cdc.gov)
Comparison of the genome sequences of phenotypically different strains could help to identify genes involved in virulence. (usda.gov)
The transmembrane domain prediction also indicated that these viral gene products contain at least one transmembrane domain in their protein sequences - with the exception of ORF6, which is actually a short protein with only 61 amino acids. (news-medical.net)
Comparison of amino acid sequences of polypeptides encoded by mau genes from M. extorquens AM1, P. denitrificans, and Thiobacillus versutus shows that they have considerable similarity. (caltech.edu)
Advances in genome resources and gene targeting have increased the attractiveness of the rat model for genetic studies but progress has been hampered by absence of relevant rat genome sequences. (europa.eu)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
We have investigated potential mechanisms of regulation of rotavirus gene expression by functional evaluation of two differentially expressed viral mRNAs. (microbiologyresearch.org)

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)
Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
These sequence variants, along with variants controlling dysregulated gene expression that we characterised previously, most likely include the major determinants of SHR cardiovascular and metabolic disease phenotypes. (europa.eu)

Further functional annotation identified conserved protein domains and assigned gene ontology terms to the PUTs. (figshare.com)
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes. (yeastgenome.org)

[ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
Each coding sequence (CDS) was amplified for 25 cycles with gene-specific primers and high-fidelity polymerase, minimizing PCR-induced mutations risk. (horizondiscovery.com)
Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)
The various forms of Waardenburg syndrome (WS), a neurocristopathy, arise from mutations in multiple genes. (medscape.com)
[ 6 ] Therefore, mutations in the PAX3 gene could affect regulation of the MITF gene, leading to abnormalities of melanocyte differentiation. (medscape.com)
Most, if not all, cases of WS1 are caused by mutations in the PAX3 gene located on chromosome band 2q35. (medscape.com)
Novel mutations of PAX3 , MITF , and SOX10 genes have been described in Chinese patients with WS1 or WS2. (medscape.com)
Mutations in the MITF gene, located on chromosome band 3p14.1-p12.3, cause some cases of WS2. (medscape.com)

The mauA, mauD, and mauE genes from Paracoccus denitrificans and the mauD and mauG genes from Methylophilus methylotrophus W3A1 were able to complement corresponding mutants of M. extorquens AM1, confirming their functional equivalence. (caltech.edu)
We shall determine the functional consequences of these variants by creating and phenotyping transgenic and knockout rats on the SHR and WKY genetic backgrounds, using transposon-mediated transgenesis and zinc-finger nuclease-mediated gene deletion recently shown to be highly efficient in rats. (europa.eu)
Here, we present a genome-wide analysis of the CDPK gene family in maize for the first time, and this genomic analysis of maize CDPK genes provides the first step towards a functional study of this gene family in maize. (biomedcentral.com)
This member alters functional properties of the KCNA4 gene product. (ucsc.edu)

Genes will be prioritised for study by statistical and informatic analyses using our extensive physiological, gene expression and linkage data in these rat strains, and by comparative analysis with data from human genome-wide association studies. (europa.eu)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Although its basic precursors (3-amino-5-hydroxy benzoic acid AHBA, and acetic and propionic acids) had been established, and several biosynthetic intermediates had been identified, very little was known about the origin of AHBA nor had the PKS and the various genes and enzymes that modify the initial intermediate been characterized. (nih.gov)

This is particularly true when expressing eukaryotic genes in bacterial hosts. (neb.com)

Six genes were cloned and the open reading frames responsible for the suppressor activity were identified. (duke.edu)

376 bp of C9orf43 are antisense to spliced gene POLE3, indicating possible regulation of alternate expression. (wikipedia.org)

Curated mutant alleles for the specified gene, listed alphabetically. (yeastgenome.org)

The SHR genome contains over 750 genes that are completely or partly deleted, or have a frameshift in their open reading frame. (europa.eu)

The arrangement of proposed genes relative to possible promoters and terminators suggests 90 potential transcription units. (nih.gov)
Transcription of an early trypsin gene, controlled by juvenile hormone, is part of the normal post-emergence maturation of the midgut in these females. (usda.gov)
Euchromatin is a lightly packed form of chromatin (DNA, RNA, and protein) that is enriched in genes, and is often (but not always) under active transcription. (bcgsc.ca)
methylates H3, including histones in the HOX area for allowing chromatin to be open to transcription. (atlasgeneticsoncology.org)
[ 4 ] The 6 genes involved in WS are PAX3 (encoding the paired box 3 transcription factor), MITF (microphthalmia-associated transcription factor), EDN3 (endothelin 3), EDNRB (endothelin receptor type B), SOX10 (encoding the Sry bOX10 transcription factor), and SNAI2 (snail homolog 2), with different frequencies. (medscape.com)

however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. (ucsc.edu)

We measured the levels of gene 5 and gene 6 mRNA and showed that they were not significantly different, and protein analysis indicated no difference in stability of NSP1 compared with VP6. (microbiologyresearch.org)
Polyribosome analysis showed that the majority of gene 6 mRNA was present on large polysomes. (microbiologyresearch.org)
In contrast, sedimentation of more than half of the gene 5 mRNA was subpolysomal. (microbiologyresearch.org)
The change in distribution of gene 5 mRNA in polyribosome gradients in response to treatment with low concentrations of cycloheximide suggested that gene 5 is a poor translation initiation template compared with gene 6 mRNA. (microbiologyresearch.org)
The Arc gene, which contains remnants of a structural GAG retrotransposon sequence, produces a protein that self-assembles into capsid-like structures harboring Arc mRNA. (lu.se)

E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
R. D. Possee, S. C. Howard, (1987) Analysis of the polyhedrin gene promoter of the Autographa californica nuclear polyhedro-sis virus. (scirp.org)

Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. (ucsc.edu)

We showed that sarcolamban, encoding two smORFs of 28 and 29aa, is the true homologue of the vertebrate genes sarcolipin and phospholamban. (cabd.es)

Expression of latency-related (LR) gene products is controlled by a 980-bp fragment (LR promoter). (unl.edu)

The molecular steps in the replication pathway that regulate the levels of viral gene expression are not well defined. (microbiologyresearch.org)
These data define a regulatory mechanism for the difference in amounts of VP6 and NSP1 and provide evidence for post-transcriptional control of rotavirus gene expression mediated by the translational efficiency of individual viral mRNAs. (microbiologyresearch.org)
Furthermore, stable viral gene expression was done in cells and subsequently verified by immunoblotting technique. (news-medical.net)

Most maize CDPK genes exhibited different expression levels in different tissues and developmental stages. (biomedcentral.com)

ORF-2 contains genes encoding the capsid. (medscape.com)
Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. (microbiologyresearch.org)

Positional cloning of lymphopenia ( lyp ) in the BB rat revealed a frameshift mutation in Gimap5 , a member of at least seven related GTPase Immune Associated Protein genes located on rat chromosome 4q24. (hindawi.com)
Our data substantiates the Gimap5 frameshift mutation as the primary defect with only limited contributions to lymphopenia from the remaining Gimap genes. (hindawi.com)
Positional cloning of the lyp gene revealed a frame shift mutation in Gimap5 (previously known as Ian5 or Ian4L1 ). (hindawi.com)
Gimap5 was identified as the lyp gene in the BBDP rat through a frameshift mutation and premature truncation of the Gimap5 protein [ 2 , 6 ] and can be rescued in a P1-derived artificial chromosome (PAC) transgenic rat [ 7 ]. (hindawi.com)

S. Gomi, C. E. Zhou, W. Y. Yih, K. Majima, Maeda S. (1997) Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV. (scirp.org)
Gene reduction analysis revealed that the gene deletion processes are under selective pressure, and many of the inactivations are probably related to the organism's interaction with its host environment. (asm.org)

Disruption of these genes resulted in suppression of the defects of the ste12Delta cells, including low mating efficiency and formation of large vacuoles. (duke.edu)

emm typing is based on sequence analysis of the portion of the emm gene that dictates the M serotype. (cdc.gov)
W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)

The green ash transcriptome we have developed provides important sequence information, genetic markers and stress-response candidate genes. (figshare.com)
A combination of de novo transcriptome assembly and DGE analysis was shown to be a powerful method for identifying genes encoding enzymes potentially involved in the biosynthesis of important secondary metabolites in a non-model plant. (biomedcentral.com)

A short open reading frame upstream of the main open reading frame may reduce efficiency of translation. (wikipedia.org)

The male sterilizing factors usually consist of unusual genes or open reading frames encoded by the mitochondrial genome. (frontiersin.org)

It indi-cated that the products of the polh gene ex-pressed in this system can be easier to use for raising antibodies. (scirp.org)

Recent genetic studies have identified hundreds of susceptibility genes for common human diseases but genetic effects are small and identifying underlying mechanisms remains challenging. (europa.eu)

Most of the mutant genes turned out to be recessive. (duke.edu)

However, expression problems are most often due to open reading frames with non-optimal codons for E. coli. (neb.com)

Additionally, 193 cytochrome P450 (CYP450), 280 methyltransferase and 144 isomerase genes were identified, that are potential candidates for enzymes involved in RIN and IRN synthesis. (biomedcentral.com)

The involvement of some of the genes in the biosynthesis of rifamycin B was examined. (nih.gov)

Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)

The nucleotide sequence of the methylamine utilization (mau) gene region from Methylobacterium extorquens AM1 was determined. (caltech.edu)

Penalty of 0.5% subtracted from overall percentage identity score for each out of frame codon. (cdc.gov)
While nine spCas9 gene editing sites surround the Arc start codon, the accuracy of the editing was highly sequence-dependent, with only a single target resulting in an in-frame reporter integration. (lu.se)

Rifamycin production in A. mediterranei is governed by a single gene cluster consisting of structural, resistance and export, and regulatory genes. (nih.gov)
The mauJ product is the only polypeptide encoded by the mau gene cluster which is predicted to be cytoplasmic. (caltech.edu)

The Open reading frame (ORF) of the AnpeNPV consists of 738 nucleotides encoding 245 amino acids with molecular masses of 29 kDa. (scirp.org)

This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)
Our data have provided evidence for a comparable prediction of clinical outcome in CMF-treated breast cancer patients using conventional clinical variables and gene expression based markers. (lu.se)
Evidence suggests that the MITF gene transactivates the tyrosinase gene, which is involved in melanocyte differentiation. (medscape.com)

Expression analyses suggested that maize CDPK genes are important components of maize development and multiple transduction pathways. (biomedcentral.com)

A) SARS-CoV-2 genome annotation, predicting 29 virus gene products. (news-medical.net)
The result showed that the bacterium expression system was suitable for the virus gene expression. (scirp.org)

The three define an ancient gene family and their peptides are structurally and functionally homologous. (cabd.es)

Anatomy11

Organisms50

Diseases3

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Psychiatry and Psychology1

Phenomena and Processes104

Disciplines and Occupations1

Information Science5

Health Care1

ORFs10

Proteins11

Encode4

CDNA4

Putative1

Encodes5

Sequences8

Differentially expressed2

Phenotypes4

Ontology3

Mutations8

Functional4

Strains2

Biosynthetic1

Bacterial1

Suppressor1

Regulation1

Alleles1

Genome contains1

Transcription5

Ribosomal protein1

MRNA5

Polyhedrin gene2

Transcript1

Vertebrate1

Promoter1

Viral3

Developmental1

Capsid2

Mutation4

Deletion2

Disruption1

Sequence Analysis2

Transcriptome2

Upstream1

Consist1

Antibodies1

Human Diseases1

Mutant1

Codons1

Enzymes1

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Nonstructural1

Nucleotide sequence1

Codon2

Cluster2

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Homologous1