• systematic name β-D-galactoside galactohydrolase), is a glycoside hydrolase enzyme that catalyzes hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. (wikipedia.org)
  • The active site of β-galactosidase catalyzes the hydrolysis of its disaccharide substrate via "shallow" (nonproductive site) and "deep" (productive site) binding. (wikipedia.org)
  • β-galactosidase , also called lactase , beta-gal or β-gal , is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond . (wikidoc.org)
  • This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. (thermofisher.com)
  • This hydrolysis is catalyzed by the enzyme lactase, a β-galactosidase located in the brush border membrane of small intestinal enterocytes. (go.jp)
  • This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. (megazyme.com)
  • Overview: Lactose utilization in GapMind is based on MetaCyc pathway lactose degradation II via 3'-ketolactose ( link ), pathway III via beta-galactosidase ( link ), or uptake by a PTS system followed by hydrolysis of lactose 6'-phosphate. (lbl.gov)
  • Plant beta-galactosidases ( EC 3.2.1.23 ) (lactases). (embl.de)
  • A few are evolved β-galactosidase (EBG), β-glucosidase, 6-phospho-β-galactosidase, β-mannosidase, and lactase-phlorizin hydrolase. (wikipedia.org)
  • About 70% of the adult world population is lactose-intolerant, due to low levels of intestinal lactase, also called lactase-phlorizin hydrolase (LPH), a β-D-galactosidase found in the apical surface of the intestinal microvilli. (researchgate.net)
  • In the 1980s, Goldstein demonstrated type B to type O conversion using α-galactosidase enzymes. (cap.org)
  • The GlcNac (β1-6) residue is the only anomeric configuration that can effect the specificity of the enzyme enabling cleavage of the non-reducing β1-4 Galactose. (neb.com)
  • The presence of sialic acid (but not fucose) linked to the galactose will block cleavage. (ludger.com)
  • Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose. (ludger.com)
  • The 4-aminophenyl releases upon cleavage by β-galactosidase. (goldbio.com)
  • 4-Nitrophenyl-α-D- galactopyranoside is a chromogenic substrate yielding a soluble chromophor for the colorimetric assay of alpha-D-galactosidase upon cleavage. (goldbio.com)
  • Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. (thermofisher.com)
  • Krabbe disease, also known as globoid cell leukodystrophy or galactosylceramide lipidosis, is an autosomal-recessive sphingolipidosis caused by deficient activity of the lysosomal hydrolase galactosylceramide beta-galactosidase (GALC). (medscape.com)
  • The LacZ protein codes for an enzyme called β-galactosidase, which is an essential part of the metabolism of lactose. (presenternet.com)
  • Also, "evolved beta-galactosidase" ebgA from E. coli is more active with its partner ebgC, but it retains activity on its own, so it is included in step lacZ instead. (lbl.gov)
  • It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. (novusbio.com)
  • The amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. (wikipedia.org)
  • Non-reducing terminal β(1-4)-Galactose. (ludger.com)
  • GALC degrades galactosylceramide, a major component of myelin, and other terminal beta-galactose-containing sphingolipids, including psychosine (galactosylsphingosine). (medscape.com)
  • Beta-gal is inhibited by L-ribose and by competitive inhibitors 2-phenylethyl 1-thio-β-D-galactopyranoside (PETG), D-galactonolactone, isopropyl thio-β-D-galactoside (IPTG), and galactose. (wikipedia.org)
  • [3] Beta-gal is inhibited by L-ribose , non-competitive inhibitor iodine , and competitive inhibitors phenylthyl thio-beta-D-galactoside (PETG), D-galactonolactone, isopropyl thio-beta-D-galactoside (IPTG) , and galactose. (wikidoc.org)
  • An acid optimum beta-D-galactoside galactohydrolase (β-galactosidase) derived from a non-GMO strain of Aspergillus oryzae . (enzymedevelopment.com)
  • 4-Aminophenyl-β-D-galactopyranoside is a substrate for β-galactosidase. (goldbio.com)
  • β-Galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. (wikipedia.org)
  • There are currently no images for beta Galactosidase Antibody (NB100-2619V). (novusbio.com)
  • Find the beta Galactosidase antibody that fits your needs. (thermofisher.com)
  • Antibodies that detect beta Galactosidase can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA and Immunoprecipitation. (thermofisher.com)
  • Our beta Galactosidase polyclonal and monoclonal antibodies are developed in Rabbit, Mouse and Chicken. (thermofisher.com)
  • These antibodies have been verified by Knockdown and Relative expression to confirm specificity to beta Galactosidase. (thermofisher.com)
  • In recent years, β-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikipedia.org)
  • One example is GOS, which are synthesized from lactose by enzymatic trans-galactosylation using β-galactosidases, achieving a degree of polymerization between 3 and 10 (Van Leeuwen et al. (springer.com)
  • The production of GOS typically involves enzymatic transgalactosylation of galactose using β-Galactosidase. (reportsanddata.com)
  • It is also a weak β-D-galactosidase inducer and can be used in affinity chromatography for isolation of galactose-binding. (goldbio.com)
  • Galactosylation is the first chemical step in the reaction where Glu461 donates a proton to a glycosidic oxygen, resulting in galactose covalently bonding with Glu537. (wikipedia.org)
  • Reactions (A), (B) and (C) contained 2 units, 4 units and 8 units of β1-4 Galactosidase, respectively, and either 1X G4 or 1X G6 reaction buffer in a total reaction volume of 10 µl. (neb.com)
  • Reaction (A) contained 24 units of α1-3,6 Galatosidase and 100 units of Neuraminidase ( NEB #P0720 ), followed by a heat kill at 65°C for 10 minutes and a 2 hour digestion with 16 units of β1-4 Galactosidase ( NEB #P0730 ). (neb.com)
  • The reaction in (B) contained 4 units of α1-3, 6 Galactosidase, 1X G6 reaction buffer and 1X BSA in a total reaction volume of 20 µl. (neb.com)
  • β-Galactosidase can catalyze three different reactions in organisms. (wikipedia.org)
  • Reactions (B), (C) and (D) were treated with 8 units of β1-4 Galactosidase prior to treatment with β-N-Acetylglucosaminidase. (neb.com)
  • β-Galactosidase has many homologues based on similar sequences. (wikipedia.org)
  • Comment: The tagatose 6-phosphate pathway involves the isomerization of galactose 6-phosphate to tagatose-6-phosphate (by lacAB), phosphorylation to tagatose 1,6-bisphosphate (by lacC), and an aldolase. (lbl.gov)
  • Many adult humans lack the lactase enzyme, which has the same function as β-galactosidase, so they are not able to properly digest dairy products. (wikipedia.org)
  • β-Galactose is used in such dairy products as yogurt, sour cream, and some cheeses which are treated with the enzyme to break down any lactose before human consumption. (wikipedia.org)
  • When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. (megazyme.com)
  • In one, it can go through a process called transgalactosylation to make allolactose, creating a positive feedback loop for the production of β-galactose. (wikipedia.org)
  • The strain was unusual because it was negative for the production of lysine decarboxylase and β-galactosidase (ortho-nitrophenol test) and positive only for stx 1. (cdc.gov)
  • Or, lactose is oxidized to 3'-ketolactose by a periplasmic 3-component dehydrogenase (lacACB'), and then hydrolyzed by a periplasmic enzyme (klh) to 3-keto-beta-D-galactose and D-glucopyranose, and hypothetical reduction of the 3-ketogalactose. (lbl.gov)
  • Other blood types differ from type O in the presence of an additional sugar antigen, α- N-acetyl galactose for type A, α-galactose for type B, or both for type AB, on the core of the nonimmunogenic H-antigen (Figure 1). (cap.org)