• cause apoptosis
  • The goal of this study was to determine whether plasmid expressed Bcr-Abl could cause apoptosis of K562 cells when specifically directed to the nucleus via strong nuclear localization signals (NLSs). (pubmedcentralcanada.ca)
  • In the nucleus, Bcr-Abl may cause apoptosis due to nuclear Abl's ability to stabilize p73 and activate its pro-apoptotic functions. (pubmedcentralcanada.ca)
  • When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
  • inhibits
  • Subsequent clinical trials led by Dr. Brian J. Druker at Oregon Health & Science University in collaboration with Dr. Charles Sawyers and Dr. Moshe Talpaz demonstrated that STI-571 inhibits proliferation of BCR-ABL-expressing hematopoietic cells. (wikipedia.org)
  • The drug is most commonly an organic small molecule that activates or inhibits the function of a biomolecule such as a protein, which in turn results in a therapeutic benefit to the patient. (wikipedia.org)
  • cells
  • When transfected into K562 cells, only 4NLS-Bcr-Abl translocated to the nucleus. (pubmedcentralcanada.ca)
  • After washout of Gleevec, Bcr-Abl's tyrosine kinase activity is re-activated, and the cells undergo spontaneous apoptosis. (pubmedcentralcanada.ca)
  • Growth arrest of BCR-ABL positive cells with a sequence-specific polyamide-chlorambucil conjugate. (nih.gov)
  • The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. (wikipedia.org)
  • There are many different cellular components involved in the cycle of apoptosis such as, BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
  • Like in HIV's replication cycle, these proteins are needed for viral budding from the host cell's plasma membrane, where the fully formed virions leave the cell to infect other cells. (wikipedia.org)
  • As their name implies, heat shock proteins protect cells when stressed by elevated temperatures. (wikipedia.org)
  • They account for 1-2% of total protein in unstressed cells. (wikipedia.org)
  • However, when cells are heated, the fraction of heat shock proteins increases to 4-6% of cellular proteins. (wikipedia.org)
  • This protein was first isolated by extracting proteins from cells stressed by heating, dehydrating or by other means, all of which caused the cell's proteins to begin to denature. (wikipedia.org)
  • oncogene
  • In the cytoplasm Bcr-Abl acts as an oncogene by interacting with multiple signal transduction pathways that transmit anti-apoptotic and mitogenic signals[ 14 ]. (pubmedcentralcanada.ca)
  • [ 5 ] KIT is a member of the receptor tyrosine kinase family of proteins, a transmembrane protein encoded by the c- kit proto-oncogene. (medscape.com)
  • The gag-onc fusion protein (also written as Gag-v-Onc, with "v" indicating that the Onc sequence resides in a viral genome) is a general term for a fusion protein formed from a group-specific antigen ('gag') gene and that of an oncogene ('onc'), a gene that plays a role in the development of a cancer. (wikipedia.org)
  • interaction
  • Homotypic interaction between specific domains of the partner protein leads to dimerization or oligomerization of the fusion protein mimicking the normal process of ligand-mediated dimerization and resulting in constitutive activation of the tyrosine kinase moiety. (haematologica.org)
  • Barilá D, Superti-Furga G. An intramolecular SH3-domain interaction regulates c-Abl activity. (springer.com)
  • cytoplasmic
  • Being a cytoplasmic protein requires that the protein be globular in structure, that is largely non-polar on the inside and polar on the outside, so as to be dissolved by water. (wikipedia.org)
  • isoform
  • It has also been shown to bind (in a different conformation from the VEGF binding) to the BCR-ABL fusion protein, specifically inhibiting the drug-resistant T315I mutant isoform. (wikipedia.org)
  • PHLPP1α, which was the first PHLPP isoform to be characterized, lacks the N-terminal portion of the protein, including the Ras association domain. (wikipedia.org)
  • inhibitor
  • They used Gleevec to stimulate Bcr-Abl to go to the nucleus (by unknown mechanism), followed by nuclear entrapment by leptomycin B (LMB), a general inhibitor of nuclear export. (pubmedcentralcanada.ca)
  • Here we show that the BCR-ABL/Src kinase inhibitor dasatinib decreases PRH phosphorylation and increases PRH-dependent repression of Vegf and Vegfr-1. (biomedsearch.com)
  • cellular
  • It deals with the structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules. (wikipedia.org)
  • Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. (wikipedia.org)
  • As a specific case, a Gag-v-Onc fusion protein from the Rous sarcoma virus is useful in illustrating the dual role that the fusion protein plays in both the viral and host cellular life cycle. (wikipedia.org)
  • Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species. (wikipedia.org)
  • tumor
  • Mislocalization of tumor suppressors, oncogenes, or factors involved in apoptosis result in aberrant functioning of these proteins, leading to disease. (pubmedcentralcanada.ca)
  • Presently, the majority of tumor biomarkers are proteins or peptides. (antibodies-online.com)
  • It also stabilizes a number of proteins required for tumor growth, which is why Hsp90 inhibitors are investigated as anti-cancer drugs. (wikipedia.org)
  • sequence
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • This typically involves removing the stop codon from a cDNA sequence coding for the first protein, then appending the cDNA sequence of the second protein in frame through ligation or overlap extension PCR. (wikipedia.org)
  • That DNA sequence will then be expressed by a cell as a single protein. (wikipedia.org)
  • The protein can be engineered to include the full sequence of both original proteins, or only a portion of either. (wikipedia.org)
  • Hsp90 is highly conserved and expressed in a variety of different organisms from bacteria to mammals - including the prokaryotic analogue HtpG (high-temperature protein G) with 40% sequence identity and 55% similarity to the human protein. (wikipedia.org)
  • tumors
  • Most but not all of these substances are proteins that can be measured in body fluids, tumors, or other tissues depending on the kind and the stage of various cancerous conditions. (antibodies-online.com)
  • signaling pathway
  • A biomolecular target (most commonly a protein or nucleic acid) is a key molecule involved in a particular metabolic or signaling pathway that is associated with a specific disease condition or pathology or to the infectivity or survival of a microbial pathogen. (wikipedia.org)
  • isoforms
  • Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. (cancerindex.org)
  • receptor
  • The conventional PKCs can be activated by calcium and diacylglycerol, two important mediators of G protein-coupled receptor signaling. (wikipedia.org)
  • decrease
  • Other mechanisms for resistance include Bcr-Abl amplification or overexpression, clonal evolution, a decrease in Gleevec bioavailability or cell exposure, and upregulation of drug efflux pumps[ 6 , 10 ]. (pubmedcentralcanada.ca)
  • This mechanism is thought to be more efficient, resulting in an approximately 80% to 95% decrease in the protein and mRNA expression. (wikipedia.org)