Further cytotoxicity drug screening assays antitumor. Medical search. Frequent questions

The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
The aim of this study was to prepare and characterize new ruthenium NAMI-type compounds, to test their in vitro cytotoxicity and study the influence of electroporation on the cytotoxic activity of the synthesized compounds. (hindawi.com)
Triticum aestivum L. root growth inhibition test and Allium cepa L. assay were used to assess the cytotoxic potential of phosphonates. (japsonline.com)
Both ethyl acetate extract and alkaline ethyl acetate extract were tested for phytochemical content by qualitative analysis and evaluated for their biological activities such as antimicrobial (by agar well diffusion assay and minimum inhibitory concentration determination) and cytotoxic activities (by MTT assay in cell culture). (biomedres.info)

Our in vitro, ex vivo and in vivo results demonstrated that such antibody was able to promote depletion of the malignant cells by antibody-dependent cell-cytotoxicity or -phagocytosis. (hipi-lab-saint-louis.fr)

Sphingolipid-targeting drugs have been tested alone or in combination with chemotherapy, exhibiting antitumor activity alone and in synergism with chemotherapy in vitro and in vivo . (frontiersin.org)
Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
Integration of a machine learning-derived signature of response with in vitro assessment of LP-184 efficacy facilitated the derivation of manageable yet robust biomarkers which can be used to predict drug sensitivity with high accuracy and clinical value. (biomedcentral.com)
Tetrazolium reduction and resazurin assays are the mainstay of routine in vitro toxicity batteries. (cdc.gov)

Cells had been treated with raising dosages of either NVP-BEZ235 or GDC-0980 and viability and development inhibition were assayed by measuring mitochondrial activity at 72?h using an MTT assay. (biomasswars.com)
Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
FDA-approved drugs were screened for their ability to sensitize TRAIL resistant prostate cancer cells to TRAIL using an MTT assay for cell viability. (biomedcentral.com)

These results demonstrate differential sensitivity of standard cytotoxicity assessments on the PPP, thus (1) decoupling 'mitochondrial activity' as an interpretation of cellular formazan and Alamar Blue metabolism, and (2) demonstrating the implicit requirement for investigators to sufficiently verify interaction of these methods in routine cytotoxicity and proliferation characterization. (cdc.gov)

It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)
The major concern of chemotherapy is the side effects of existing drugs ( Baldo and Pham, 2013 ). (japsonline.com)
Our finding supports PDL-1 in CAFs as a chemotherapy response biomarker and as a drug delivery and therapeutic target for chemoresistant NSCLC. (cdc.gov)

Mab-ZAP can be utilized for screening mouse IgG antibodies for internalization and/or their suitability to make potent immunotoxins. (atsbio.com)
It had cytotoxicity against human lung carcinoma A549 cell line and was stable up to 100 °C. Thus, the study revealed that the strain A. fumigatus nHF-01 produces a potent broad-spectrum AMC 5-butyl-2-pyridine carboxylic acid that could be used against human food and topical pathogenic bacteria. (nature.com)
Potent cell growth inhibitory properties were shown by ligands MS47 and MS49 against human melanoma MDA-MB-435, colon cancer HCT-116 and COLO 205, and pancreatic cancer MIA PaCa-2 cell lines, as evidenced by MTT assay. (openmedicinalchemistryjournal.com)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)

In order to confirm the effect of the extract on motility of human endothelial cells, cell migration assay was conducted. (emanresearch.org)

To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
The phytochemical screening detected the presence of saponins, alkaloids, reducing sugars and coumarins. (researchgate.net)

Since discovering the life-supporting drug, Penicillin, the first β -lactam antibiotic, by Sir Alexander Fleming in the 1920s, lots of progress have been developed in the antibacterial compounds production, process development and characterization of active molecules. (nature.com)

This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)
The methanolic extracts of seaweeds Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata possess high total phenolic content and shows a good free radical scavenging activity and hence are proven to have better antioxidant activity and they might be good candidates for further investigations in order to develop potential anticancer drugs. (google.com)
Natural products from plants and microbial sources, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drugs discoveries due to unmatched chemical diversity 1 . (nature.com)
L. egeregia was regarded as the prototype of the anticancer species due to its profound flavonoid concentration (85.40 µg/mL) and cytotoxicity (9.46 µg/mL) compared to other extracts. (who.int)

Compounds of interest include not only classical "drug-like" organic small molecules, but also peptides, proteins, and other bioactive chemical classes. (cancer.gov)
Cytotoxicity and anticancer potential of newly synthetisized compounds were investigated. (japsonline.com)
The present work focused on (1) synthesis of β-amino- and β-iminophosphonates via a nucleophilic addition reaction of benzylamine and 2-aminopirydine to 1,2-alkadienephosphonates, i.e., dialkyl esters and amidoesters, as well as dimethyl- and diphenylphosphine oxides, and (2) investigation of cytotoxicity and anticancer potential of synthesized compounds. (japsonline.com)

Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines. (google.com)
Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)

It has antiproliferative and antitumor activities, as well as induction of apoptosis and the inflammatory response. (sigmaaldrich.com)
T umor Necrosis Factor-α R elated A poptosis I nducing L igand (TRAIL) and agonistic antibodies to death receptor 4 and 5 are promising candidates for cancer therapy due to their ability to induce apoptosis selectively in a variety of human cancer cells, while demonstrating little cytotoxicity in normal cells. (biomedcentral.com)
In the current work, we screened a small library of fifty-five FDA and foreign-approved anti-neoplastic drugs in order to identify candidates that sensitized resistant prostate and pancreatic cancer cells to TRAIL-induced apoptosis. (biomedcentral.com)
Sequential and simultaneous dosing modalities were investigated and the annexin V/propidium iodide assay, in concert with fluorescence microscopy, was employed to visualize cells undergoing apoptosis. (biomedcentral.com)
TRAIL has attracted significant attention in recent years due to its ability to selectively induce apoptosis in transformed (malignant) cells while demonstrating little cytotoxicity in normal cells [ 2 - 7 ]. (biomedcentral.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)

Results Drug sensitivity screening of mesothelioma cell lines In this study we aimed at determining systems accounting for level of sensitivity level of resistance towards dual PI3K/mTOR inhibitors in a big -panel of mesothelioma cell lines. (biomasswars.com)

Discuss the current challenges and key considerations to be taken when designing assays to identify new biomarkers in pan tumor applications. (hub-xchange.com)

It shows cytotoxicity with GI50 values ranging from 0.05 to 1 μm in different malignant cell lines with an average value of 0.35 μm. (rgcb.res.in)
These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
Explore how novel tissue staining technologies can be applied to interrogate new drug targets and immune cell while preserving tissue architecture for spatial analysis. (hub-xchange.com)
To be able to address this relevant query we performed a cytotoxicity display in 19 commercially obtainable mesothelioma cell lines. (biomasswars.com)
The activity is determined by the dose-dependent cytotoxicity of the human TF-1 cell line (human erythroleukemic indicator cell line)) ≤ 0.4. (sigmaaldrich.com)
In this study, lentiviral transfection of sh‑RNA was used to knock down the level of RRS1, to detect the effect of RRS1 on cell function and to explore the specific mechanism of RRS1 affecting cell invasion and metastasis by COIP and dual‑luciferase reporter gene assays. (morulaivf.com)
Drugs demonstrating the highest synergy were selected as leads and tested in different prostate and pancreatic cancer cell lines, and one immortalized human pancreatic epithelial cell line. (biomedcentral.com)
Open data on the gene expression of the NCI-60 cell lines, provides a unique opportunity to add another dimension to the preclinical development of such drugs by interrogating correlations with gene expression patterns. (biomedcentral.com)
LN metastases resist T cell-mediated cytotoxicity, induce antigen-specific regulatory T cells, and generate tumor-specific immune tolerance that subsequently facilitates distant tumor colonization. (stanford.edu)
Its activation can be monitored in real-time by following the formation of fluorescent ASC specks and measuring pyroptotic cell death with the LDH assay. (invivogen.com)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)

Finally, the antitumor effect of the leaves ethanolic extract was evaluated using in vivo human tumor xenograft model. (emanresearch.org)
Chemotherapeutic drugs target cancer-specific characteristics, such as alterations in DNA repair, which has complicated roles in oncogenesis and tumor progression. (biomedcentral.com)
When loaded with immune complexes (IC), consisting of tumor antigens bound to antitumor antibody, BMDC induce powerful antitumor immunity in mice. (stanford.edu)

Aslthough all these drugs have produced good results in preclinical studies of multiple cancers, the outcomes of clinical trials have not been similar. (frontiersin.org)
GDC-0980 continues to be tested in stage I studies where in fact the stage I expansion cohort demonstrated two objective reactions among 26 individuals with mesothelioma.17 Despite these motivating outcomes this drug will never be explored further due to side effects seen in another clinical trial.18 This however shouldn't deter us for looking for way to enhance the antitumor aftereffect of this course of agents. (biomasswars.com)

NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
Pharmacophore-based activity profiling screen demonstrated some biological targets that MS44-53 may modulate their biological response, and thus can be considered as potential drugs to treat different kinds of diseases, such as carcinoma, diabetes type II, bacterial infection and cardiovascular diseases. (openmedicinalchemistryjournal.com)
Using a dual luciferase assay, we assessed the effect of JMJD5 on the transcriptional activity of the c-Myc target gene. (morulaivf.com)
The pharmacological screening studies indicated that the aqueous extract of Z. spina-christi root bark has an antinociceptive activity in mice and rats [ 23 ] and a central depressant effect in mice [ 24 ]. (biomedres.info)

assays indicated possible low cy to to xicity to the extract. (researchgate.net)
Hexane extract of the stem bark and ethanolic leaves extract profoundly inhibited the sprouting of microvessels (by 89.56% and 87.12% respectively) in rat aortic ring assay. (emanresearch.org)

This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
The development of biomimetic nanoparticles (NPs) has revolutionized the concept of nanomedicine by offering a completely new set of biocompatible materials to formulate innovative drug delivery systems capable of imitating the behavior of cells. (frontiersin.org)
Fourteen drugs were identified as having synergy with TRAIL, including those whose TRAIL sensitization activities were previously unknown in either prostate or pancreatic cancer cells or both. (biomedcentral.com)
GFP with the NF-κB inducer TNF-α and stimulation using SARS-CoV-2 infectious particles, A549-ASCoV2-NLRP1 cells display ASC specks, visible as fluorescent green dots and undergo pyroptosis, readily measurable by the LDH assay. (invivogen.com)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

M. Gaba, C. Mohan, Development of drugs based on imidazole and benzimidazole bioactive heterocycles: recent advances and future directions. (dntb.gov.ua)

The Molecular Targets Program (MTP) provides the focus and infrastructure that enables CCR investigators to pursue molecularly targeted drug discovery research by promoting an interdisciplinary, collaborative, team-oriented approach to identifying and validating potential cancer-pertinent targets. (cancer.gov)

Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)

The objective of this review was to analyze the results from preclinical and clinical trials of these drugs for the treatment of cancer. (frontiersin.org)
The effectiveness or lack of a major therapeutic effect of sphingolipid modulation by some drugs as a cancer therapy and other aspects related to their mechanism of action are discussed in this review. (frontiersin.org)
Cancer AND drug name. (clinicaltrialsregister.eu)
The search for targeted drugs for cancer treatment includes consideration of natural products, which may provide new opportunities for antitumor cytotoxicity as single agents or in combination therapy. (cancer.gov)
These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
The identification of a number of FDA-approved drugs as TRAIL sensitizers can expand chemotherapeutic options for combination treatments in prostate and pancreatic cancer diseases. (biomedcentral.com)
The Part 2 post in this series is concerned with mainline anti-cancer drug interventions that simultaneously address multiple growth pathways, ones that are entering clinical practice. (anti-agingfirewalls.com)
In particular, the discovery of the new use of artemisinin derivatives as excellent anti-cancer drugs is also reviewed. (anti-agingfirewalls.com)

The current investigation aimed to demonstrate how interpretation of results from several standard cytotoxicity and proliferation assays vary in dependence on contributions from the pentose phosphate pathway (PPP). (cdc.gov)

Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
We performed an overexpression screen with 41 human proteases to identify which deubiquitinases stabilize c-MYC. (morulaivf.com)

Overall, our study offers a novel conceptual framework for biomimetic NPs using a DoE strategy and consolidates the high therapeutic potential of leukosomes as a viable drug delivery system for anti-inflammatory and antineoplastic applications. (frontiersin.org)

Brad Nelson has twenty five years of experience in leading rapid growth life science organizations, delivering new technology and applications for drug discovery, development and clinical markets. (hub-xchange.com)
Joseph Krueger serves as the scientific leader for Invicro's Advanced Pathology Services and is responsible for developing new biomarker applications to meet drug development needs. (hub-xchange.com)
Prior to joining Invicro, Joseph was Chief Scientific Officer at Flagship Biosciences where he led the R&D and Scientific efforts in applying novel quantitative tissue image analysis approaches to immuno-oncology and rare diseases to support drug and companion diagnostic development. (hub-xchange.com)
Prior to Flagship Biosciences, he served in senior scientific roles in oncology drug development for OSI Pharmaceuticals and Pfizer. (hub-xchange.com)
Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
The plant can be used as promising candidate for anti-neoplasic drug development. (emanresearch.org)

Drug Metab Dispos 5:198-204. (cdc.gov)

The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. (scialert.net)

Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)

The need to control all these variables often leads to time-consuming and very expensive screening studies that require the analysis of a single variable at a time. (frontiersin.org)

Various anti-oxidants have found to possess properties such as anti-atherosclerotic, antitumor, anti-mutagenic, anti-carcinogenic to name a few selected ones [ 4 ]. (biomedcentral.com)

Analysis of variance was used to identify drugs that exhibited synergy with TRAIL. (biomedcentral.com)

To develop mAbs to potentially target oncofetal antigens and be repurposed for antibody or antibody drug conjugate (ADC) therapy. (atsbio.com)

Anatomy25

Organisms14

Diseases33

Chemicals and Drugs117

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes36

Disciplines and Occupations1

Technology, Industry, Agriculture5

Information Science1

Brine Shrimp Lethality Assay4

Cytotoxic5

Antibody-dependent ce1

Vitro5

Viability3

Mitochondrial activity1

Chemotherapy3

Potent4

Endothelial cells1

Phytochemical2

Antibacterial1

Extracts4

Compounds3

Anticancer4

Apoptosis6

Sensitivity1

Biomarkers1

Cell11

Tumor3

Outcomes2

Activity5

Extract2

Cells5

Bioactive1

Targets1

Compound2

Cancer8

Demonstrate1

Human2

Therapeutic1

Development6

Dispos1

Biological activities1

Immune1

Studies1

Properties1

Identify1

Therapy1