Functional normal mice mice knockout. Medical search. Frequent questions

To address this question, we created Treg-specific Cd28 conditional knockout mice. (jci.org)
Oocyte-specific, beta1 integrin conditional knockout mice allowed us to obtain mature eggs lacking. (lu.se)
Importantly, the regenerating epithelium around the DUM demonstrated heightened STAT3 phosphorylation and cell proliferation, which was suppressed in uteri of Stat3 conditional knockout mice. (jci.org)
To explore VHL as a regulator defining nephron progenitor self-renewal versus differentiation, we bred Six2-TGC tg mice with VHL lox/lox mice to generate mice with a conditional deletion of VHL from Six2 + nephron progenitors. (lww.com)
Conditional knockout of Gli3 causes a significant larger brain size, which can be recovered by knockdown of miR-7. (frontiersin.org)

Sperm and testis parameters of homozygous KO adult male mice were analyzed and compared with those of wild-type animals. (nih.gov)
We showed that homozygous KO males were fertile and displayed normal sperm parameters and a functional spermatogenesis. (nih.gov)
Furthermore, a knock-in mouse model confirmed that heterozygous or homozygous mutations within Zfp513 (p.R250W) or Kif3c (p.R412H) alone do not led to clear phenotypes with gingival fibromatosis, whereas the double mutations led to gingival hyperplasia phenotypes. (nature.com)
gem homozygous receptor mutant HCs display normal cell viability, afferent synaptogenesis, and peripheral innervation, yet exhibit strongly reduced extracellular potentials (∼50% of wild-type potentials). (jneurosci.org)
Intriguingly, LTP was normal in TEC slices from heterozygous knockouts of the alternate VPS26a subunit, whereas homozygous VPS26a knockouts died in utero. (alzforum.org)
White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (wikipedia.org)

By contrast, targeted deletion of genes such as Math1 (a mouse homolog of Drosophila proneural gene atonal ) or sequences encoding BRN 3.1 and BDNF produces congenital sensorineural anomalies of the inner ear. (nih.gov)
For example, the identification of regulatory sequences of genes expressed specifically in the vestibular system will allow the targeted introduction of foreign genes into the vestibular system of transgenic mice. (nih.gov)
To verify the implication of these candidate genes in NOA, we used the CRISPR/Cas9 system to invalidate the mouse orthologs 4930522H14Rik and Cct6b and produced two knockout (KO) mouse lines. (nih.gov)
To discover cell and molecular underpinnings of circuit tuning, her lab has conducted functional screens for genes regulated by neural activity. (stanford.edu)
Despite focusing on functional studies of correlatively pathogenic genes, less research has been done to investigate possibilities of their counter-regulatory genes as potential modulators of brain morphogenesis and therapeutic targets of malformation. (frontiersin.org)
Because Drosophila lack most of these redundant genes, we examined heart tubes of parkin knockout flies and observed accumulation of enlarged hollow donut mitochondria with dilated cardiomyopathy, which could be rescued by cardiomyocyte-specific Parkin expression. (wustl.edu)
A Female medaka lacking foxl3 genes, looking very much like a normal female medaka - but her ovaries (lower images) tell a different story. (discovermagazine.com)
Surprisingly, animals deficient for BOB.1 / OBF.1 showed normal expression of genes that contain an octamer motif in their regulatory regions. (nih.gov)
Humans share many genes with mice. (wikipedia.org)

Through the study of genetic mutations in mouse that result in no b-wave ERG phenotypes, several proteins have been identified that play crucial roles in On BC signal transmission. (louisville.edu)
They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (wikipedia.org)
In persons with autosomal dominant DKC and in terc-/- knockout mice, genetic anticipation (ie, increasing severity and/or earlier disease presentation with each successive generation) has been reported. (medscape.com)

To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)
By embryonic day 15.5, kidneys of nephron progenitor cell-specific VHL knockout mice begin to exhibit reduced maturation of nephron progenitors. (lww.com)
Our findings identify a novel role for VHL in mediating nephron progenitor differentiation through metabolic regulation, and suggest that VHL is required for normal kidney development. (lww.com)
Knockdown of microRNA (miRNA) miR-7 can rescue abnormal brain morphology in Gli3 knockout mice by recovering progenitor specification, neuronal production and migration through a counter-balance of the Gli3 activity. (frontiersin.org)

An initially normal sensory thalamus was repatterned to match the aberrant S1 map by apoptotic deletion of thalamic neurons representing body parts with axons excluded from S1. (nature.com)
Figure 6: Top-down plasticity repatterns the VPN through exaggerated apoptosis that is selective for VPN neurons representing body parts deleted from S1 in cKO mice. (nature.com)
The findings, made in mice, explain why most but not all motor neurons are affected by the disease and identify a potential therapeutic target for this still-incurable neurodegenerative disease. (sciencedaily.com)
The neurons were taken from normal mice. (sciencedaily.com)
In the January 18 issue, Small, Beth Stevens at Boston Children's Hospital, and colleagues reported that knocking out a different retromer protein, VPS35, in hippocampal neurons in mice not only jammed neuronal endosome traffic but also caused microglia to assume shapes resembling those seen in AD. (alzforum.org)
In cultured primary neurons from mice, fluorescent antibodies to VPS26a (blue) and 26b (red) detected two distinct retromer complexes. (alzforum.org)
To see which brain cells carried which VPS26, Simoes cultured primary mouse neurons, astrocytes, microglia, and endothelial cells separately. (alzforum.org)
In paper №2, I describe how mesDA neurons transplanted in the adult SN of a PD mouse model, extended axons across millimetres into the striatum, functionally reforming the nigrostriatal pathway. (lu.se)
In paper №3, I also identify the specific mesDA population (A9) that is critical for functional recovery, with transplants that lack A9 neurons failing to improve motor recovery. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

Particularly, by performing transcriptome analysis, we observed that several pluripotent transcriptional factors increase in knockout cell line, which explains the underlying loss of pluripotency in Sirt6-null iPS-like cell line. (biomedcentral.com)
Methods and Results: Transcriptional profiling of Parkin knockout mouse hearts revealed compensatory upregulation of multiple related E3 ubiquitin ligases. (wustl.edu)

Blocking this activity in utero, or preventing vision after birth, disrupts normal tuning up of circuits and brain wiring. (stanford.edu)

Our work aims at understanding the mechanisms of leukemogenesis induced by these oncogenes, by a multidisciplinary approach that combines molecular genetics, functional genomics and proteomics with the analysis of transgenic mice or of knock-out mice. (umontreal.ca)
We observe that SCL blocks thymocyte differentiation and induces leukemia in transgenic mice by disrupting a combinatorial code between the E2A, HEB transcription factors and signalling by the pre-TCR. (umontreal.ca)

Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

In addition to identifying VHL as a critical regulator of nephron progenitors' metabolic switching, the authors' findings demonstrate that this switch also plays a large role in the differentiation process, and suggest that VHL is required for normal kidney development. (lww.com)
However, whether and how other sirtuins, especially nuclear epigenetic regulator Sirt6, regulate mouse somatic reprogramming still remains exclusive. (biomedcentral.com)

Although the existence of additional anomalies of the nervous system or other organ systems is difficult to exclude, the existing animal models are a valuable resource for understanding molecular bases for normal inner ear development. (nih.gov)
Many of these programs generated rich public data sets containing a variety of multi-dimensional molecular and phenotypic data from several organisms including mouse and human. (nih.gov)
Although a close connection between uterine regeneration and successful pregnancy in both humans and mice has been consistently observed, its molecular basis remains unclear. (jci.org)
This review will focus on the most recent/relevant molecular findings driving this functional impairment of mesenchymal stem cells in the aging process. (biomedcentral.com)
UXT deficient mice show drastic retinal defects at molecular, physiological and functional aspect [7]. (rna-seqblog.com)

OP9 feeder cell co-culture system was used to measure the hematopoietic differentiation from mouse ES and iPS cells. (biomedcentral.com)
Sirt1, the mouse homologue of yeast Sir2 deacetylates several non-histone proteins and plays roles in many key functions, including energy metabolism, differentiation, aging, and tumor suppression [ 10 ]. (biomedcentral.com)
During the preparation of our manuscript, another group reported that Sirt6 knockout ES cells skewed towards neuroectoderm differentiation [ 20 ]. (biomedcentral.com)
But the exact role of Sirt6 in mouse somatic reprogramming and iPS cell differentiation remains unrevealed. (biomedcentral.com)

By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
The first recorded knockout mouse was created by Mario R. Capecchi , Martin Evans , and Oliver Smithies in 1989, for which they were awarded the 2007 Nobel Prize in Physiology or Medicine . (wikipedia.org)

Mouse somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. (biomedcentral.com)

Here, making use of a newly generated Pdgfd knockout mouse, we reveal a functionally important malignant cell heterogeneity modulated by PDGF-DD signaling in pancreatic neuroendocrine tumors (PanNET). (lu.se)

We previously showed that peroxisome proliferator-activated receptor (PPAR)-γ in β-cells regulates pdx-1 transcription through a functional PPAR response element (PPRE). (diabetesjournals.org)

The F81Y knockin mouse has a surprising phenotype: though the point mutation shifted the λmax from 360 nm to 420 nm, it also led to 2- to 3-fold reduced expression, which we have shown is due to ER-associated degradation, which can be completely reversed by increasing 11-cis retinal exogenously. (ucdavis.edu)
It is impossible to investigate normal retinal function with 1P imaging, because the rods and cones are too sensitive to visible light. (ucdavis.edu)
Functional analyses demonstrate that the wild-type Pk1a partially suppresses the gene knockdown retinal defects but not the mutant forms. (biologists.com)
My data not only add to the literature on animal models of cCSNB, but to the understanding of retinal circuitry in the normal retina. (louisville.edu)
They knockout, overexpress and minimize the UXT gene expression to pattern its effect on retinal degeneration and compared it with normal retina by RNA sequencing analysis . (rna-seqblog.com)

Mouse embryonic fibroblasts isolated from transgenic Oct4-GFP reporter mice with or without Sirt6 were used for reprogramming by Yamanaka factors. (biomedcentral.com)
Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (wikipedia.org)

We show that the size of S1 in mice is significantly reduced by cortex-specific deletion of Pax6 , resulting in a reduced body map and loss of body representations by an exclusion of later-differentiating sensory thalamocortical input. (nature.com)
These studies will employ multiple innovative reagents including mouse models of inducible, cardiomyocyte-specific L2A deletion and overexpression, a recently developed small molecule activator of CMA, and a new CMA reporter mouse. (elsevierpure.com)

Islet GIP-R expression and immunohistochemistry studies were performed in pancreas-specific PPARγ knockout mice (PANC PPARγ −/− ), normoglycemic 60% pancreatectomy rats (Px), normoglycemic and hyperglycemic Zucker fatty (ZF) rats, and mouse islets incubated with troglitazone. (diabetesjournals.org)
In vivo verification was shown by a 70% reduction in GIP-R protein expression in islets from PANC PPARγ −/− mice and a twofold increase in islets of 14-day post-60% Px Sprague-Dawley rats that hyperexpress β-cell PPARγ. (diabetesjournals.org)
Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)

Lung inflammation was assessed following oropharyngeal aspiration of CB or oxidized CB(ox) particles (20g per mouse) in C57BL/6J mice. (cdc.gov)

We used histologic, immunofluorescence, RNA sequencing, and metabolic assays to characterize kidneys from these mice and controls during development and up to postnatal day 21. (lww.com)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
Toxicity and functional assays were also performed on murine macrophage (RAW 264.7) and endothelial cell lines (C166) with and without pharmacological inhibitors. (cdc.gov)

Knocking it out in mice slowed glutamate receptor recycling and weakened synaptic transmission, but only in the transentorhinal cortex, hinting at why this region is so vulnerable to AD pathology in people. (alzforum.org)
Indeed, LTP weakened and glutamate receptor GluA1 expression was lower in TEC brain slices, while both were normal in the medial entorhinal cortex. (alzforum.org)

C57BL6 male mice were exposed for 3hours by whole body inhalation and acute toxicity determined after 24h. (cdc.gov)

In a different experiment, they also found that functional blockade of GLAST in normal adult mice results in similar abnormalities as seen in the knockout mice. (hokudai.ac.jp)

In another experiment, the researchers tested whether MMP-9 has a functional role in ALS by crossing MMP-9 knockout mice with SOD1 mutant mice (a standard mouse model of ALS). (sciencedaily.com)
A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or " knocked out ", an existing gene by replacing it or disrupting it with an artificial piece of DNA . (wikipedia.org)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)

Using Western blot analysis, the affinity-purified polyclonal VCY2 antibody gave a single specific band of approximately 14 kDa in size, corresponding to the expected size of VCY2 in all the collected human testicular biopsy specimens with normal spermatogenesis. (bioone.org)

yet, eggs null for the alpha6 integrin exhibit normal fertilization. (lu.se)
VHL knockout nephron progenitors also exhibit persistent Six2 and Wt1 expression, as well as decreased mitochondrial respiration and prolonged reliance on glycolysis. (lww.com)
Embryos lacking maternal lilli expression show specific defects in the establishment of a functional cytoskeleton during cellularization, and exhibit a pair-rule segmentation phenotype. (sdbonline.org)

This in vivo functional impairment was accompanied by dampened expression of CTLA-4, PD-1, and CCR6. (jci.org)
In this review, we discuss the functional cross talk of proteostasis and mitostasis in cellular homeodynamics and the impairment of mitochondrial quality control during ageing, cancer, and neurodegeneration. (hindawi.com)

Objective: We used germline mutants and cardiac-specific RNA interference to interrogate Parkin regulation of cardiomyocyte mitochondria and examine functional crosstalk between mitophagy and mitochondrial dynamics in Drosophila heart tubes. (wustl.edu)
Conclusions: Parkin deficiency and resulting mitophagic disruption produces cardiomyopathy in part by contamination of the cardiomyocyte mitochondrial pool through fusion between improperly retained dysfunctional/ senescent and normal mitochondria. (wustl.edu)
The maintenance of "healthy" and fully functional mitochondria is thus essential for cellular homeodynamics. (hindawi.com)

Besides impairing ATP production by compromising the normal function of the respiratory chain machinery, excessive ROS generation and the ensuing oxidative stress cause oxidative damage to proteins, lipids, and DNA, accelerating the aging phenotype [ 5 , 6 ]. (aging-us.com)
Since the phenotype of this model is relatively modest, we developed a muscle specific FKRP knock-out mouse model, based on Cre-lox recombination technology. (mdaconference.org)

On June 21 and 22, 1999, the National Institute on Deafness and Other Communication Disorders (NIDCD) and the Life Sciences Division of the National Aeronautics and Space Administration (NASA) co-sponsored a program planning workshop, "Role of Transgenic and Knockout Studies in Understanding Sensory-Motor Performance in Altered Gravitational Performance. (nih.gov)
These results indicate that none of the integrins known to be present on mouse eggs or to be ADAM receptors are essential for sperm-egg binding/fusion, and thus, egg integrins may not play the role in gamete fusion previously attributed to them. (lu.se)
This review summarises our current state of knowledge of the functional role of TASK-1 channels in the pulmonary circulation in health and disease, with special emphasis on current advancements in the field. (ersjournals.com)
In this study, we sought to determine the role of Sirt6 in mouse somatic reprogramming. (biomedcentral.com)
By studying and/or generating knockout mice, the lab is exploring a role for these molecules in synaptic plasticity, learning, memory and neurological disorders. (stanford.edu)
To probe VPS26b's role in brain function, the scientists turned to VPS26b knockout mice. (alzforum.org)
Aim 1, will define the functional role of CMA in heart failure, using both pressure overload and MI models. (elsevierpure.com)

RNA sequencing studies can be used to characterize transcriptome of normal or diseased retina [5]. (rna-seqblog.com)

Mouse VPS26b knockouts had more Aβ, less entorhinal cortex neurotransmission, and worse memory function. (alzforum.org)

Tissue microarrays and proteomics found GPNMB to be upregulated in recurrent GBMs compared to primary (p=0.0349 and p=0.0033 respectively), particularly in macrophage populations while being absent in normal tissues. (bmj.com)

DUMs were transplanted into the mouse uteri with artificially induced defects, and all the uterine layers were recovered at the DUM transplantation sites within a month. (jci.org)
cCSNB is caused by postsynaptic defects in On bipolar cells (BCs) and is identified by the presence of an electroretinogram (ERG) with a normal a-wave, corresponding to photoreceptor function, and the absence of a b-wave, corresponding to a failure of On BC function. (louisville.edu)

The knockout males were seemingly unaffected by the loss of foxl3. (discovermagazine.com)

The same effect on motor neuron function was seen when MMP-9 was inactivated in SOD1 mutant mice using chemical injections or virally mediated gene therapy. (sciencedaily.com)
Professor Masahiko Watanabe of Hokkaido University and his colleagues in Japan compared the wiring of Purkinje cells in normal mice and mutant mice lacking GLAST. (hokudai.ac.jp)
The wiring of Purkinje cells in the mutant mice was laden with abnormalities. (hokudai.ac.jp)
However, in the mutant mice, Purkinje cells were innervated by multiple climbing fibers, which apparently caused the Purkinje cells to be atypically excited. (hokudai.ac.jp)

These include the functional co-expression of both mouse cone opsins (S- and M-) in most cones, the requirement for the G-protein receptor kinase Grk1 (formerly "rhodopsin kinase") in cone opsin deactivation, and the functional co-expression of two arrestins (Arr1, Arr4), establishing that at least one of the arresting be present for normal deactivation of both S- and M-opsin. (ucdavis.edu)
The study of RP1L1 −/− mice also showed that the RP1L1 protein is located in the axoneme of the outer segments and connecting cilia exclusively in rod photoreceptors. (molvis.org)
Glycoprotein nonmetastatic melanoma protein B (GPNMB) has previously been identified as a clinically relevant target in GBM while being absent in normal brain tissues and shown to be active in the tumor immune microenvironment. (bmj.com)
Thiazolidinedione activation (72 h) of this pathway in normal mouse islets caused a threefold increase of GIP-R protein and a doubling of insulin secretion to 16.7 mmol/l glucose/10 nmol/l GIP. (diabetesjournals.org)
For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumours by arresting cell division and/or inducing apoptosis. (wikipedia.org)

The differences between cCSNB animal models provide important clues into the functional roles of the proteins effected by the mutations. (louisville.edu)
In the presence of functional DNA replication, RTEL1 mutations produce a large amount of extrachromosomal T-circles. (medscape.com)

Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer , obesity , heart disease , diabetes , arthritis , substance abuse , anxiety , aging and Parkinson's disease . (wikipedia.org)
Resistin was downregulated in the mouse by thiazolidinediones (TZDs), which are agonists for the antidiabetic peroxisome proliferator-activated receptor γ (PPAR-γ), and was proposed to link obesity to diabetes ( 2 ). (diabetesjournals.org)

The S-opsin knockout mouse has revealed a competitive inhibition between S- and M-opsin mRNA during translation in cones that co-express both opsins, and has provided an invaluable control for many experiments in which we assess biochemical and histological properties of S-opsin. (ucdavis.edu)
The results of the study indicate that a dose of 5e12 vg/kg corrects to the wild-type level the myopathology at the histological and functional levels. (mdaconference.org)

In addition, our research aims at understanding how SCL and LMO2 influence normal hematopoietic cell fate. (umontreal.ca)

4- to 7-week-old WT or Cd28 -ΔTreg mice were pulsed with BrdU every 12 hours for 3 days and sacrificed, and relevant tissues were analyzed by flow cytometry. (jci.org)
Methods Immunohistochemistry was performed on patient derived xenograft (PDX) brains and tissue microarrays of 16 patient matched primary/recurrent GBMs as well as 23 normal organ tissues. (bmj.com)
They identified a gene which was active in the egg cells but not in the surrounding ovary tissues - foxl3 - and sought to determine its purpose by creating knockout fish lineage that lacks expression of that gene. (discovermagazine.com)
Hamartomas are malformations of mucosa, consisting of disorganized normal tissues. (czytelniamedyczna.pl)
The term of hamartoma corresponds to a non-neoplastic tumour, consisting of disorganized normal tissues and characteristic of the site of manifestation of the tumour. (czytelniamedyczna.pl)

Disease occurrence was not due to subversion of Cd28 -deficient Tregs into pathogenic cells, as complementation with normal Tregs prevented disease occurrence. (jci.org)
Interestingly, a mouse ortholog of HAS2-AS1, Has2as, clearly exhibited a differential splicing pattern among three major splice variants in the Pnn-deficient mouse cornea. (molvis.org)

We found that the beta1 integrin null eggs were fully functional in fertilization both in vivo and in vitro. (lu.se)
Functional studies demonstrated that the ZNF513 p.R250W and KIF3C p.R410H variants significantly increased the expression of ZNF513 and KIF3C in vitro and in vivo. (nature.com)

We here established a mouse model of decellularized uterine matrix (DUM) transplantation. (jci.org)
In ovariectomized mice, uterine regeneration of the DUM transplantation was similarly observed, suggesting that ovarian hormones are not essential for this regeneration process. (jci.org)
A ) A schematic illustration of a mouse model of decellularized matrix transplantation (DMT). (jci.org)
D ) A macroscopic image of the transplantation site in a recipient mouse uterus immediately after DMT (day 0). (jci.org)

Experimental procedures of a mouse DMT model. (jci.org)
Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model. (nih.gov)
This effect on nerve-muscle synapses is the largest ever seen in a mouse model of ALS," said Dr. Spiller. (sciencedaily.com)
We previously published a proof-of-principle of the efficiency of AAV-mediated transfer of the FKRP gene to correct the myopathology in a KI mouse model reproducing the most frequent mutation (L276I) in LGMD-R9. (mdaconference.org)
This new mouse model, named HSA-FKRPdel, has a normal life span but present a severe dystrophic process in the skeletal muscle. (mdaconference.org)
The HSA-FKRPdel mouse model was then used for a dose effect study of an AAV9 expressing FKRP under the expression of a muscle promoter. (mdaconference.org)
In paper №1, I detail the neurodegenerative and behavioural outcomes in a mouse lesion model of PD, which can be used as a platform for the development of novel therapeutic strategies. (lu.se)

CRISPR/Cas9 was used to eliminate expression in GBM lines to measure proliferation and mouse survival times. (bmj.com)
Eliminating GPNMB in cell lines decreased proliferation (P≤0.001) and prolonged survival times in all mouse models (P≤0.01) indicating its functional relevance. (bmj.com)

C ) BrdU incorporation in thymectomized mice, as per the protocol in A . CD4 + FOXP3 + cells from blood were analyzed at the indicated times after the last BrdU pulse. (jci.org)
Resected mouse uteri were processed with SDS to make DUMs without any intact cells. (jci.org)
Uterine epithelial cells rapidly migrated and formed a normal uterine epithelial layer within a week, indicating a robust epithelial-regenerating capacity. (jci.org)
A molecule produced by insulating glial cells facilitates the functional wiring of brain cells involved in motor coordination. (hokudai.ac.jp)
In spite of the environment surrounding the germ cells being female, the fact that functional sperm has been made surprised me greatly. (discovermagazine.com)
Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)
For this example, we will take stem cells from a white mouse. (wikipedia.org)

GPNMB knockout clones displayed downregulation of hallmark signalling pathways of GBM such as PDGFR, TGF-beta, Integrins and Stats, as well as decreased innate/adaptive immune activation. (bmj.com)

A trimmed DUM was exactly fitted into a rectangular defect in a recipient mouse uterus and fixed to the recipient uterus with intermittent sutures. (jci.org)

Woolsey, T.A. & Van der Loos, H. The structural organization of layer IV in the somatosensory region (SI) of mouse cerebral cortex. (nature.com)

In contrast, 12-month-old knockouts had less metabolic activity than controls in the same region. (alzforum.org)

Using high-resolution functional MRI, the authors found the tiny transentorhinal cortex lit up more in 3-month-old knockouts than it did in age-matched wild-type controls (see image below). (alzforum.org)

In other words, having MMP-9 is an absolute predictor that a motor neuron will die if the disease strikes, at least in mice. (sciencedaily.com)

Given the metabolic decline in the 12-month-old VPS26b knockouts, did synaptic signaling wane as well? (alzforum.org)

The generation of mutant and transgenic animal models, involving such species as the mouse, Drosophila, zebrafish and Caenorhabditis elegans (C. elegans), provides powerful tools for elucidation of these functions. (nih.gov)

Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
The demonstration of what could happen was great, and they used mice because it's a more tractable system, but what we cared about were humans. (medscape.com)

By illustration, a large number of zebrafish and mouse mutants with vestibular system involvement have been developed. (nih.gov)
Notable exceptions are the tilted and tilted head mouse mutants, having only one identified phenotypic defect, the absence of otoconia, and 100% penetrance. (nih.gov)

GPNMB knockout clones were generated in GL261 and engrafted in immunocompetent mice to examine single cell transcriptomes using sciRNAseq at endpoint. (bmj.com)

In the regenerated uteri, normal hormone responsiveness in early pregnancy was observed, suggesting the regeneration of functional uteri. (jci.org)

Anatomy3

Organisms8

Diseases1

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes1

Conditional5

Homozygous6

Genes9

Genetic3

Progenitor4

Neurons10

Transcriptional2

Utero1

Transgenic mice2

Neuronal3

Regulator2

Molecular5

Differentiation4

Physiology2

Pluripotent1

Functionally1

Transcription1

Retinal5

Embryonic2

Deletion2

Rats3

C57BL1

Assays3

Glutamate2

Toxicity1

Adult1

Researchers3

Spermatogenesis1

Exhibit3

Impairment2

Mitochondria3

Phenotype2

Role7

Transcriptome1

Neurotransmission1

Proteomics1

Defects2

Males1

Mutant mice4

Protein5

Mutations2

Obesity2

Histological2

Hematopoietic1

Tissues5

Deficient2

Vivo2

Transplantation4

Model7

Proliferation2

Cells7

Activation1

Uterus1

Cerebral1

Contrast1

Found1

Neuron1

Metabolic1

Drosophila1

Humans2

Mutants2

Examine1

Regeneration1