Function of podocytes proteins podocytes. Medical search. Frequent questions

Disruption of the filtration slits or destruction of the podocytes can lead to massive proteinuria, where large amounts of protein are lost from the blood. (wikipedia.org)
Tangshenning (TSN) has been shown to reduce proteinuria, protect renal function, and reduce podocyte damage. (hindawi.com)
Podocytes are the last barrier of the glomerular filtration membrane, and podocyte dysfunction can cause proteinuria and glomerular sclerosis, exacerbating the progression of DN [ 3 ]. (hindawi.com)
TSN has been applied clinically for many years to reduce proteinuria and improve renal function in patients with DN, and its therapeutic effects have been confirmed in clinical trials [ 10 ]. (hindawi.com)
Experimental studies have shown that the main bioactive components of TSN, astragaloside IV, calycosin, emodin, ferulic acid and quercetin have beneficial effects on diabetic nephropathy by reducing proteinuria, protecting renal function, and inhibiting renal fibrosis [ 13 - 18 ]. (hindawi.com)
Therefore, in diseases that can threaten the glomerular normal environment, the presence and the quantification of urinary podocytes can be of remarkable relevance, as it can herald or accompany the appearance of proteinuria, and could offer another view to the interpretation and clinical approach and outcome of proteinuria. (degruyter.com)
Patients with nephrotic syndrome lose too many necessary proteins into the urine (proteinuria). (petmd.com)
The direct impairment of migration capacity of renal cells by hantaviral N proteins may contribute substantially to proteinuria observed in the clinical picture of hantavirus infection. (biomedcentral.com)
Proteinuria is a major determinant of progression of chronic kidney disease (CKD), which is associated with more rapid worsening of renal function and earlier initiation of renal replacement therapy independent of estimated glomerular filtration rate (eGFR) [ 1 ]. (karger.com)
It can be classified on the basis of the amount of protein (nephrotic or non-nephrotic), the type of protein (albuminuria or low molecular weight proteinuria), or the underlying pathological damage (glomerular vs non-glomerular). (medscape.com)
Overflow proteinuria is most commonly associated with increased production of abnormal low molecular weight proteins (eg, light chains in multiple myeloma, myoglobin in rhabdomyolysis) that exceeds the reabsorption capacity of the tubules, leading to spilling of the protein into the urine. (medscape.com)
Transient proteinuria occurs in patients with normal renal function, bland urine sediment, and normal blood pressure. (medscape.com)
Patients have normal renal function and proteinuria usually is less than 1 g/day with no hematuria. (medscape.com)
Glomerular proteinuria associated with pathological damage to the glomerulus is categorized by protein quantity. (medscape.com)
3.5 g of proteinuria on a spot urine protein-to-creatinine ratio. (medscape.com)
Individuals with kidney disease-associated ACTN4 mutations tend to have mild to moderate proteinuria, with many developing decreased kidney function progressing to end stage kidney disease. (biomedcentral.com)
Specifically, our findings indicate that hic-5 protects podocytes from cell death by mediating cell cycle control through stabilization of p21 after genotoxic stress in vitro and in vivo, thereby maintaining the structural integrity of the glomerular filtration barrier, ameliorating proteinuria and preventing glomerular scarring. (grantome.com)
Alport syndrome is a rare genetic kidney disorder caused by mutations in COL4A3/4/5 genes, characterized by podocyte injury and impaired kidney filter function leading to proteinuria. (ozarksfirst.com)
These findings are generally consistent with previous assessments, providing stronger evidence of protein restoration and the disease modifying effect of ELX-02 and potential for improvement in proteinuria with longer duration of treatment. (ozarksfirst.com)
Manifestations include insidious onset of edema and heavy proteinuria with benign urinary sediment, normal renal function, and normal or elevated blood pressure. (msdmanuals.com)
The most prominent of these developmental defects is usually a dramatic reduction in late glomerular maturation, which appears to involve mis-regulation of VEGF-A production from podocytes subsequently leading to proteinuria and decreased glomerular filtration rate (GFR). (biodiversityhotspot.org)

The podocytes have long foot processes called pedicels, for which the cells are named (podo- + -cyte). (wikipedia.org)
The foot processes known as pedicels that extend from the podocytes wrap themselves around the capillaries of the glomerulus to form the filtration slits. (wikipedia.org)
A loss of the foot processes of the podocytes (i.e., podocyte effacement) is a hallmark of minimal change disease, which has therefore sometimes been called foot process disease. (wikipedia.org)
Here, we show that Lmx1b(-/-) podocytes have reduced numbers of foot processes, are dysplastic, and lack typical slit diaphragms, indicating an arrest in development. (nih.gov)
Our results indicate that reduced levels of proteins associated with foot processes and the glomerular slit diaphragm likely contribute, along with reduced levels of GBM collagens, to the nephropathy associated with NPS. (nih.gov)
podocytes run the risk of detachment from the basement membrane due to a decrease in the adherence to the surrounding matrix after contraction, a process called foot processes effacement. (degruyter.com)
DN advanced phenotyping will include measures of podocyte number, precise morphometric analysis of glomerular expansion, glomerular volume and tubulointerstitial fibrosis, EM morphometry of podocyte foot processes, immunohistochemical analysis of podocyte specific proteins, and glomerular isolation. (mmpc.org)
and 2) Examine whether the presence of hic-5 in podocyte foot processes results in the activation of a pro-survival signature in podocytopathies in vivo by use of an innovative super-resolution immunofluorescence microscopy technique to validate our findings in human disease. (grantome.com)
Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. (joplink.net)
The epithelial cells, also known as podocytes, have specialized foot processes embedded in the outer layer of the GBM. (basicmedicalkey.com)
Podocytes deficient in Lmx1b fail to develop foot processes and have defective basement membrane formation (15). (biodiversityhotspot.org)

In 2002 Professor Moin Saleem at the University of Bristol made the first conditionally immortalised human podocyte cell line. (wikipedia.org)
The human podocyte cell line was derived from human normal podocytes conditionally transformed with a temperature-sensitive mutant of the simian virus 40 (SV40) large T antigen. (biomedcentral.com)

Recent evidence suggests that the factor could be released by T-cells or B-cells, podocyte cell lines can be treated with plasma from patients with nephrotic syndrome to understand the specific responses of the podocyte to the circulating factor. (wikipedia.org)
NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome. (scienceopen.com)
Nephrotic syndrome is a condition where the kidney's filtering units are damaged, allowing large amounts of protein that should be kept in the bloodstream to leak into the urine. (nstrust.co.uk)
Using this technique, the team were able to replace the original faulty gene in the podocytes, successfully treating several different laboratory-based models of nephrotic syndrome. (nstrust.co.uk)
Circulating relapse EVs are biologically active molecules that carry active RAC1 as cargo and induce recapitulation of the NS phenotype in podocytes in vitro.NEW & NOTEWORTHY Up to now, the role of extracellular vesicles (EVs) in the pathogenesis of steroid-sensitive nephrotic syndrome (NS) has not been studied. (izon.com)
Wt1 is the earliest marker of the podocyte, and depending upon the degree of Wt1 deficiency a spectrum of kidney defects can arise from adult-onset nephrotic syndrome to complete renal agenesis in Wt1? (biodiversityhotspot.org)

KLF family members are expressed in most if not all tissues and have diverse roles in organismal development and cell differentiation, function, and death. (jci.org)
Thus, LMX1B regulates the expression of multiple podocyte genes critical for podocyte differentiation and function. (nih.gov)
Experiments were performed with non-proliferating, differentiated podocytes expressing synaptopodin as podocyte-specific marker of differentiation. (biomedcentral.com)
We conclude that similar to those TFs mentioned above, Ebf1 is also an essential regulator of podocyte differentiation and glomerular maturation. (biodiversityhotspot.org)
Podocyte differentiation and glomerulogenesis in fetal life is controlled by specific genes and any disturbance of this process may lead to further structural defects with clinical consequences. (czytelniamedyczna.pl)
The WT1 protein mediates the mesenchymal-epithelial transition and differentiation during morphogenesis of the kidney and gonad by repressing genes that encode cell proliferation factors and by activating genes that encode markers of epithelial cell differentiation. (medscape.com)
Point mutations in the WT1 gene result in loss of its regulatory function, with the consequent abnormalities in glomerular formation and gonadal differentiation seen in Denys-Drash syndrome. (medscape.com)

When infants are born with certain defects in these proteins, such as nephrin and CD2AP, their kidneys cannot function. (wikipedia.org)
Nephrin is a zipper-like protein that forms the slit diaphragm, with spaces between the teeth of the zipper big enough to allow sugar and water through but too small to allow proteins through. (wikipedia.org)
The slits are covered by slit diaphragms which are composed of a number of cell-surface proteins including nephrin, podocalyxin, and P-cadherin, which restrict the passage of large macromolecules such as serum albumin and gamma globulin and ensure that they remain in the bloodstream. (wikipedia.org)
Proteins that are required for the correct function of the slit diaphragm include nephrin, NEPH1, NEPH2, podocin, CD2AP. (wikipedia.org)
Results showed that TSN treatment enhanced the weakened nephrin expression and autophagic activity of podocytes and inhibited the mTORC1 pathway (p-mTOR, mTOR, p-p70S6K, p70S6K, ULK1, and 4EBP1) under high glucose conditions. (hindawi.com)
Using antibodies to podocyte proteins important for podocyte function, we found that Lmx1b(-/-) podocytes express near-normal levels of nephrin, synaptopodin, ZO-1, alpha3 integrin, and GBM laminins. (nih.gov)
This study suggested that WSJPR could effectively relieve renal damage and improve renal function of DN rats by ameliorating metabolism disorder and increasing the gene expression of nephrin and podocin, which might be a useful approach for the treatment of DN. (biomedcentral.com)
Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair. (musc.edu)

However, mRNA and protein levels for CD2AP and podocin were greatly reduced, suggesting a cooperative role for these molecules in foot process and slit diaphragm formation. (nih.gov)
NPHS2 is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. (scienceopen.com)
We found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier. (scienceopen.com)
Lmx1b and FoxC combinatorially regulate podocin expression in podocytes. (ox.ac.uk)
Podocin is a key protein of the kidney podocyte slit diaphragm protein complex, an important part of the glomerular filtration barrier. (ox.ac.uk)
The findings, which were published in Science Translational Medicine by the team in Bristol led by Professors Moin Saleem and Gavin Welsh, suggested that replacing one faulty gene that codes for a protein known as podocin could cure the condition. (nstrust.co.uk)
Podocin is a protein essential for the functioning of cells called podocytes which have a critical role within the kidney's filtration system. (nstrust.co.uk)

Co-overexpression of the two genes potently induced endogenous nphs2 expression in zebrafish podocytes. (ox.ac.uk)
Our results show that podocyte expression of some genes is combinatorially regulated by two transcription factors interacting synergistically with a common enhancer. (ox.ac.uk)
Exons 1-6 of the WT1 gene encode the regulatory domain, which regulates expression of target genes, and exons 7-10 encode the 4 zinc fingers of the DNA-binding region of the WT1 protein. (medscape.com)

CD2AP regulates the podocyte cytoskeleton and stabilizes the slit diaphragm. (wikipedia.org)

Podocytes are cells in Bowman's capsule in the kidneys that wrap around capillaries of the glomerulus. (wikipedia.org)
We propose the outline of such a mechanism based on following aspects: (1) The glomerulus is a complex structure, the stability of which depends on the cooperative function of the basement membrane, mesangial cells and podocytes, counteracting the distending forces originating from the high glomerular hydrostatic pressures. (scienceopen.com)
We were probably the first to describe the electron microscopy finding of the SARS-CoV-2 virus in kidney proximal tubular cells and also in podocytes of the glomerulus. (medscape.com)

Bowman's capsule filters the blood, retaining large molecules such as proteins while smaller molecules such as water, salts, and sugars are filtered as the first step in the formation of urine. (wikipedia.org)
This phylogenetically preserved cell that is responsible for the virtually absence of proteins in the urine lacks of the capacity to divide under normal conditions. (degruyter.com)
Noteworthy, the podocytes that are encountered in the urine are viable despite the glomerular hostile environment and the urinary acidity. (degruyter.com)
Neph1 is essential for the maintenance of podocytes--neuron-like cells with long finger-like projections that wrap around the glomerular capillaries, serving as the final barrier between the blood and the urine. (sciencedaily.com)
When podocyte structure fails, the kidney is no longer able to act as an effective filter, and excessive protein escapes the capillaries into the urine. (sciencedaily.com)
The glomeruli are penetrable clusters of capillaries in the kidney that function to filter waste from the blood, establishing the formation of urine, one of the body's main methods for disposing of waste products. (petmd.com)
Also, since proteins essential for breaking down blood clots are lost into the urine, the blood coagulates much more easily and blood clots can become lodged in blood vessels, causing paralysis or strokes. (petmd.com)
Protein electrophoresis may help to identify which proteins are being lost into the urine through the kidneys so that a prognosis can be established. (petmd.com)
Your veterinarian may prescribe medication to stop the loss of protein into your dog's urine and to increase its blood pressure. (petmd.com)
The chemical blood profile is useful for monitoring kidney function, and the urinalysis will indicate the amount of protein being lost into the urine. (petmd.com)
The primary outcome was 24-h urine protein reduction. (karger.com)
Amiloride reduced 24-h urine protein by 38.7% ( p = 0.002) and decreased systolic BP by 12.3 mm Hg ( p = 0.04). (karger.com)
Interestingly, triamterene reduced 24 h urine protein as well, by 32.8% ( p = 0.02). (karger.com)
These patients require close follow-up and may need a kidney biopsy if they have abnormal urine microscopy results and/or impairment of kidney function. (medscape.com)
Normal urine protein excretion is up to 150 mg/day. (medscape.com)
Therefore, the detection of abnormal quantities or types of protein in the urine is considered an early sign of significant renal or systemic disease. (medscape.com)
The detection of various types of proteins excreted in the urine has been extensively used in the assessment of renal diseases. (medscape.com)
As previously announced, Patient 4401-02 achieved remission based on Urine-Protein Creatine ratio (UPCR). (ozarksfirst.com)
Renal function, sex and age influence purines and pyrimidines in urine and could lead to diagnostic misinterpretation. (uib.no)

The podocytes form one layer of the filtration barrier. (wikipedia.org)
Genetic mutations can cause podocyte dysfunction leading to an inability of the filtration barrier to restrict urinary protein loss. (wikipedia.org)
It is present in cow's milk and beef protein and can escape the intestinal barrier and cause antibody formation. (medscape.com)
The glomerular filtration barrier mainly exerts its function via three layers: fenestrated endothelium, glomerular basement membrane (GBM), and podocytes. (biomedcentral.com)
MAGI-2 scaffold protein is critical for kidney barrier function. (nephcure.org)
The kidneys play a major role in the retention of plasma proteins, using renal tubules to reabsorb them as the proteins pass through the glomerular filtration barrier. (medscape.com)
Hic-5, a member of the LIM domain family of proteins, is increased in podocytes along the glomerular filtration barrier in human podocytopathies. (grantome.com)
Some of the glomerular cells, such as the epithelial cells, have phagocytic function that can remove macromolecules trapped within the filtration barrier. (basicmedicalkey.com)

Moreover, relapse EVs induced significantly higher levels of RAC-GTP and phospho-p38 and decreased the levels of synaptopodin in podocytes. (izon.com)

The glomerular podocyte is particularly sensitive to mitochondrial dysfunction, as seen in various genetic disorders manifesting as progressive glomerulosclerosis. (jci.org)
Podocyte-specific deletion of Klf6 expression in mice leads to mitochondrial dysfunction and apoptosis, followed by glomerulosclerosis. (jci.org)
Disruption of the exocyst induces podocyte loss and dysfunction. (musc.edu)
Conditional Loss of the Exocyst Component Exoc5 in Retinal Pigment Epithelium (RPE) Results in RPE Dysfunction, Photoreceptor Cell Degeneration, and Decreased Visual Function. (nih.gov)

Fatty acid-binding protein 3 (FABP3) located in renal mesangial and distal tubular cells, and had been shown to be a sensitive marker of renal injury, potentially be a mediator in pathogenesis of chronic kidney disease (CKD). (medsci.org)

Millions of people are affected by irreversible loss of renal function and thus by a significantly increased cardiovascular risk. (scienceopen.com)
There is a presence of crescents around the nephron which can lead to rapid and progressive loss of renal function. (freezingblue.com)

Podocytes make up the epithelial lining of Bowman's capsule, the third layer through which filtration of blood takes place. (wikipedia.org)
Podocytes are also involved in regulation of glomerular filtration rate (GFR). (wikipedia.org)
When podocytes contract, they cause closure of filtration slits. (wikipedia.org)
Researchers used small-angle X-ray scattering to determine the full structure of the motor protein Myo1c and its complex with Neph1, a protein crucial for kidney filtration. (sciencedaily.com)
The motor protein Myo1c binds to Neph1, a protein crucial for ensuring effective filtration by the kidney, and serves as one mode of its cellular transport, according to findings by investigators at the Medical University of South Carolina (MUSC) and their collaborators reported in the May 16, 2016 issue of Molecular and Cellular Biology . (sciencedaily.com)
Faulty podocyte structure and filtration also characterize diabetic nephropathy and glomerular diseases (e.g., focal and segmental glomerular sclerosis), which were once considered orphan diseases but have become more prevalent in recent years as sedentary lifestyles and diets high in processed foods have become commonplace. (sciencedaily.com)
When filtration cells (podocytes) in the kidney's glomeruli become damaged due to either immune complexes in the blood (called glomerulonephritis), or due to dense deposits of hard protein (amyloid), abnormal accumulation of which is called amyloidosis, degeneration of the kidney's tubular system occurs. (petmd.com)
Dr. Mundel's focus is on the makeup and function of podocytes, key cells found in each of the one million separate filtration units packed into a single human kidney. (nephcure.org)
Targeting Neph1 and ZO-1 protein-protein interaction in podocytes prevents podocyte injury and preserves glomerular filtration function. (musc.edu)

We did a solution-based structure where we could actually get the structure of the entire protein and study that structure in complex with a cargo protein (Neph1). (sciencedaily.com)
When injury occurs, Neph1 and other surface proteins are dislodged into the cytoplasm and must find their way back to the surface of the cell membrane to trigger the events needed for recovery. (sciencedaily.com)
Solution structure analysis of cytoplasmic domain of podocyte protein Neph1 using small/wide angle x-ray scattering (SWAXS). (musc.edu)

Two of these proteins are albumin, which helps to maintain blood pressure and keep blood in the vessels, and antithrombin III, which prevents blood clots from forming. (petmd.com)
Relapse EVs were efficiently internalized by podocytes and induced significantly enhanced motility and albumin permeability. (izon.com)
Those patients do not respond to any antiproteinuric treatment and after short time of vigorous supplementation of protein and albumin - bound vital substances, most of the patients must finally undergo bilateral nephrectomy, to avoid life-threatening complications of massive, untreatable urinary loss of protein. (czytelniamedyczna.pl)

signaling and fibrosis in podocytes. (musc.edu)

RSV can improve mitochondrial function, reduce oxidative stress in podocytes, and inhibit apoptosis induction through the Sirt1/PGC-1a axis ( 12 ). (frontiersin.org)

You keep the same podocytes for life, so if you can change their gene expression right at the beginning of the disease, we should be able to prevent this disease from progressing. (nstrust.co.uk)
For gene therapy to be successful, researchers must make sure the new genetic material reaches the right cells and is used by those cells for a long time to restore their normal function. (nstrust.co.uk)
Gene Ontology classification analysis and Ingenuity Pathway Analysis were performed on selectively expressed EV proteins during relapse. (izon.com)
Using the Dignet dynamic gene interaction literature mining program, we have identified over 700 podocyte-associated gene-gene interactions . (cellcards.org)
Furthermore, we have developed a SciMinor and BioBERT-based literature mining program, and used it to identify more podocyte-associated gene interactions from the PubMed literature. (cellcards.org)
Young adult with Inherited gene defect chromosome 16 with protein called polycystin-1. (freezingblue.com)
So-called syndromic nephrosis is mainly presented by infants with other co-morbidities, however it should be stressed, that it may be diagnosed also in adolescent or adult patients, as it is in Charcot-Marie-Tooth disease, where there is a link between INF2 gene mutation, formin protein, podocytes and Schwann-cell function (the last causing severe neuropathy) (3). (czytelniamedyczna.pl)
The precise ratio of the KTS-positive/negative isoforms seems to be crucial for the normal function of the WT1 gene. (medscape.com)

Accumulation of α-synuclein mediates podocyte injury in Fabry nephropathy. (uib.no)

Experimental research found a markedly decreased expression of autophagy-related proteins such as Beclin-1, Atg12-Atg5, and LC3-II in a diabetic mouse model and in a high glucose-induced podocyte injury model [ 4 ]. (hindawi.com)
mTORC1 activation inhibits autophagy, and it is considered to be a molecular marker of diabetic nephropathy [ 7 , 8 ], as the decrease in podocyte autophagic activity caused by mTORC1 activation plays a vital role in the progression of this disease [ 9 ]. (hindawi.com)
More importantly, astragaloside IV (the main component of Astragali Radix) and emodin (the main component of Rhei Radix Et Rhizoma) ameliorate podocyte damage in diabetic nephropathy by enhancing podocyte autophagy [ 13 , 16 ]. (hindawi.com)
Role of podocyte B7-1 in diabetic nephropathy. (nephcure.org)
where Mdm2 was revealed as a relevant downregulated protein in the diabetic kidney disease. (uni-muenchen.de)
In conclusion, therapeutic Mdm2 blockade may hold the risk of damaging renal function in patients with no underlying kidney diseases and also in those with diabetic nephropathy. (uni-muenchen.de)

In zebrafish, double knockdown of Lmx1b and FoxC orthologs using morpholino doses that caused no or minimal phenotypic changes upon individual knockdown completely disrupted podocyte development in 40% of injected embryos. (ox.ac.uk)

Although various viscera have epithelial layers, the name visceral epithelial cells usually refers specifically to podocytes, which are specialized epithelial cells that reside in the visceral layer of the capsule. (wikipedia.org)
Hantaviral N protein was detected in tubular and glomerular cells and cell-to-cell contact structures were changed in biopsy samples of patients with serologically confirmed PUUV infections [ 3 ]. (biomedcentral.com)
when podocytes are lost for any reason they cannot be replaced by new cells. (scienceopen.com)
Its administration in a mouse AKI model showed a diminished capacity of healing and the specific deletion of Mdm2 in both podocytes and tubular cells induced p53-overexpression dependent cell death (podoptosis). (uni-muenchen.de)
For this reason we hypothesized that the administration of Nutlin-3a to healthy mice damages renal function by promoting podoptosis in podocytes and tubular cells. (uni-muenchen.de)
Consistent with the hypothesis, we observed that stimulation with Nutlin-3a increased cell death in cultured mouse podocytes and tubular cells. (uni-muenchen.de)
Podoplanin is expresed in kidney podocytes, type 1 lung alveolar cells and lymphatic endothelial cells. (ox.ac.uk)
In the present study, we evaluated the content of oxidized proteins and phospholipid fatty acid profiles of EVs released by human bronchial epithelial BEAS-2B cells treated with CSE. (izon.com)
After confirmation that CSE reduces viability of BEAS-2B cells and elevates intracellular ROS levels, in a dose-dependent manner, we demonstrated that 24 h exposure at 1% CSE, a concentration that only slight modifies cell viability but increases ROS levels, was able to increase carbonylated protein levels in cells and released EVs. (izon.com)
The release of oxidatively modified proteins via EVs might represent a mechanism used by cells to remove toxic proteins in order to avoid their intracellular overloading. (izon.com)
a new one was just discovered - but the main one that the spike protein uses in order to enter the cells is angiotensin converting enzyme (ACE) type 2 . (medscape.com)

DN progression is associated with podocyte damage due to reduced autophagy caused by mTORC1 activation. (hindawi.com)
Podocyte damage caused by weak autophagic activity is an essential mechanism underlying DN progression [ 5 ]. (hindawi.com)
Progression of glomerular diseases: is the podocyte the culprit? (scienceopen.com)
Pathologically, podocyte loss remains the single unifying observation and closely correlates with disease progression. (grantome.com)

Normally, activation of the CB 1 receptor regulates renal vascular hemodynamics and stimulates the transport of ions and proteins in different nephron compartments. (degruyter.com)

Understanding the role of hic-5 in preventing podocyte loss by regulating the podocyte cell cycle may guide our efforts to discover pathways to nephron loss, identify prognostic biomarkers and develop targeted podocyte-specific therapies for proteinuric kidney diseases. (grantome.com)
Young adults and children, often associated with HIV, heroin use, igA nephropathy and maladaption of nephron loss damage to podocyte initiates to global sclerosis. (freezingblue.com)

Neutral endopeptidase, a podocyte antigen that can digest biologically active peptides, was identified as the target antigen in a subset of patients with antenatal membranous nephropathy. (medscape.com)

Podocytes secrete and maintain the basement membrane. (wikipedia.org)
The podocyte is a highly differentiated cell located in the outer space of the glomerular basement membrane that deals with many different functions. (degruyter.com)
Podocytes change their shape and under constant mechanical stress they finally detach, rendering the glomerular basement membrane unprotected unless other contiguous podocytes are capable of covering the surface. (degruyter.com)

Results Ebf1-deficient mice have decreased GFR While examining Ebf1s role in osteoblast function (6) we identified an incongruity between the quality of bone, and the level of circulating osteocalcin (Ocn). (biodiversityhotspot.org)
Fig. 1 GFR Is certainly LOW IN Mice And Accompanied By Albuminuria The main path of clearance for Ocn may be the Mocetinostat kidney, and its own circulating amounts correlate inversely with reduced renal function where it could be 2C200xs greater than amounts in healthy people (18C22). (biodiversityhotspot.org)
The noticed GFR uncovered an 80% decrease in kidney function in mice in comparison to their handles (Fig. 1D). (biodiversityhotspot.org)

In that case, Myo1c could be an attractive therapeutic target, as inhibiting it would prevent these proteins from leaving the cell membrane, an event that triggers the injury response. (sciencedaily.com)
The Use of High-Throughput Transcriptomics to Identify Pathways with Therapeutic Significance in Podocytes. (musc.edu)
Ginseng ( Panax ginseng C. A. Mey) has a wide range of their therapeutic functions anti-stress, health promotion, maintaining and enhancing central and immune systems, preventing certain chronic diseases, as well as aging deterrent properties. (frontiersin.org)

Hakroush S, Cebulla A, Schaldecker T, Behr D, Mundel P, Weins A. Extensive podocyte loss triggers a rapid parietal epithelial cell response. (nephcure.org)

The exclusive expression of NPHS2 in podocytes reflects its unique function and raises interesting questions about its transcriptional regulation. (ox.ac.uk)
Here, we further define a 2.5-kb zebrafish nphs2 promoter fragment previously described and identify a 49-bp podocyte-specific transcriptional enhancer using Tol2-mediated G0 transgenesis in zebrafish. (ox.ac.uk)

In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. (thermofisher.com)
Relapse NS EVs selectively expressed proteins that involved actin cytoskeleton rearrangement. (izon.com)

Primary podocytes (Lonza) were maintained in RPMI medium (Capricorn) supplemented with 10% fetal calf serum (FCS). (biomedcentral.com)

When podocytes receive molecular insults, which normally occur during stress conditions as glomerulonephritis, hyperfiltration or metabolic disturbances, they adapt to the new situation by contracting their actin fibers. (degruyter.com)
The translated protein has a molecular weight of about 60 kD and exhibits NAD + -dependent deacetylase activity. (frontiersin.org)
It comprises low molecular proteins such as beta-2 microglobulin, which in normal conditions are completely reabsorbed by proximal tubules. (medscape.com)
These low molecular proteins can be toxic to the tubules and can cause acute kidney injury. (medscape.com)
Podocyte death has recently gained acceptance as a key pathway to podocyte loss, although the molecular mechanisms remain elusive. (grantome.com)

The impact of hantaviral nucleocapsid proteins (N proteins) on motility was examined by transfection of podocytes. (biomedcentral.com)
The impaired motility depends on viral replication and transfection of podocytes with N protein of PUUV or HTNV reveals that the expression of N protein alone is sufficient to deteriorate podocyte function. (biomedcentral.com)
Here, we found that relapse NS EVs contain significantly increased active RAC1, induce enhanced podocyte motility, and increase expression of RAC-GTP and phospho-p38 expression in vitro. (izon.com)

DR advanced testing will include measures of retinal vascular permeability, retinal cell death and non-lethal measures of retinal morphology using optical coherence tomography and visual function using optokinetic response. (mmpc.org)
Bradykinin decreases podocyte permeability through ADAM17-dependent epidermal growth factor receptor activation and zonula occludens-1 rearrangement. (musc.edu)

Normal kidney filter function depends on the integrity of GBM and of tubular and glomerular monolayers. (biomedcentral.com)

The quantitative protein excretion is less than 1 g/day. (medscape.com)

Several proteins are required for the pedicels to wrap around the capillaries and function. (wikipedia.org)
Podocytes have primary processes called trabeculae, which wrap around the glomerular capillaries. (wikipedia.org)

Still, the effect of TSN on the autophagic activity of podocytes remains unclear. (hindawi.com)
Podocytes depend on high autophagic activity to maintain their physiological functions [ 6 ]. (hindawi.com)
We found Plk1 plays a critical role in maintaining intralysosomal pH by regulating ATP6V1A phosphorylation, and inhibition of Plk1 impaired lysosomal function leading to blockade of autophagic flux. (nature.com)

The M-type phospholipase A2 receptor (PLA2R) in the glomerular podocyte has been identified as the major target antigen in deposited immune-complexes. (msdmanuals.com)

Herein, in vitro experiments using a high glucose-induced podocyte injury model were performed. (hindawi.com)
To solve that mystery, Nihalani and his collaborators have developed a mouse model in which Myo1c is knocked out in the podocytes and are conducting experiments to better understand the effects of Myo1c knockout on the injury/recovery response. (sciencedaily.com)
The overarching goal of this proposal is to elucidate the pro-survival role of hic-5 following podocyte injury by studying its effect on cell cycle checkpoint control. (grantome.com)

In patients with idiopathic FSGS, investigational findings for an underlying etiology-such as systemic lupus erythematosus (serum complement C4/C3 levels, antinuclear antibody/anti-DNA titers), hepatitis B or C or HIV infection, vasculitis (antineutrophil cytoplasmic antibody titers, serum protein electrophoresis)-are generally negative. (medscape.com)
ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)
The C-type lectin-like protein (CLEC-2) is a type 2 transmembrane protein with an extracellular C-type lectin-like domain and a short cytoplasmic domain containing a single YXXL motif. (ox.ac.uk)

Renal replacement therapy (RRT) is yet the primary treatment for end-stage renal disease (ESRD) patients that does not actually improve kidney function and also creates a heavy economic burden in several countries [ 1 , 2 ]. (nature.com)

Both CB 1 and CB 2 receptors, which share a low level (44%) of sequence homology [6], are G protein-coupled receptors that mainly signal via G i /G o proteins, even though they may also activate G s , G q/11 , and G protein-independent signaling pathways [ 7 ]. (degruyter.com)

All of the disease-causing ACTN4 mutations identified to date are located within the actin-binding domain of the encoded protein, increasing its binding affinity to F-actin and leading to abnormal actin rich cellular aggregates. (biomedcentral.com)
There are four human α-actinin (ACTN1-4) family members, encoding closely related actin crosslinking proteins. (biomedcentral.com)
Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. (joplink.net)

Estrogen decreases tight junction protein ZO-1 expression in human primary gut tissues. (musc.edu)

Glomerular epithelium cell (podocyte) (at protein level). (joplink.net)
Other sites of abnormal cell vacuolization include the renal glomerular podocytes and in the fibroblasts of the liver's periportal spaces. (medscape.com)

Several studies have determined that the extent of tubulointerstitial involvement is correlated better with renal function deterioration than glomerular changes. (nature.com)

Anatomy11

Organisms1

Diseases13

Chemicals and Drugs11

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Proteinuria21

Foot processes11

Human podocyte cell line2

Nephrotic syndrome6

Differentiation7

Nephrin8

Podocin7

Genes3

Slit diaphragm1

Glomerulus3

Urine19

Barrier8

Synaptopodin1

Dysfunction4

Mesangial1

Loss of renal function2

Filtration9

NEPH13

Albumin3

Fibrosis1

Mitochondrial function1

Gene8

Mediates1

Diabetic6

LMX1B1

Cells11

Progression4

Regulates1

Nephron loss2

Biologically active1

Basement membrane3

Mice3

Therapeutic3

Epithelial1

NPHS22

Cytoskeleton2

Fetal1

Molecular5

Motility3

Permeability2

Kidney filter function1

Excretion1

Capillaries2

Autophagic3

Antigen1

Injury3

Cytoplasmic3

Patients1

Pathways1

Actin3

Decreases1

Cell2

Tubulointerstitial1