• The bacterial population structure of waste before and after treatment was monitored using 16S rRNA gene sequence libraries. (cdc.gov)
  • Another related tool, RDP Library Compare, was developed to facilitate microbial-community comparison based on 16S rRNA gene sequence libraries. (asm.org)
  • For example, a complete 16S rRNA gene sequence can be obtained by analyzing the sequences of genomes or large genome fragments, providing taxonomic information along with information on other functional genes. (frontiersin.org)
  • Each coding sequence (CDS) was amplified for 25 cycles with gene-specific primers and high-fidelity polymerase, minimizing PCR-induced mutations risk. (horizondiscovery.com)
  • An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
  • Extensive DNA sequence comparisons between various Xenopus histone gene clusters were undertaken. (bl.uk)
  • An artificial microRNA (amiFATB) was created by replacing a microRNA sequence in the camelina Csa-miR159a gene with a FATB gene specific sequence. (osti.gov)
  • We recommend taking a plurality consensus sequence for each gene segment, genome, and/or lineage/subtype. (cdc.gov)
  • Usage of reporter genes need to be specified in iLab before project start and the exact sequence information and gene co-ordinates need to be submitted to be included during sequencing read alignment. (lu.se)
  • Mutations in the IFNGR1 gene can lead to extreme susceptibility to Mycobacterial infections. (wikidoc.org)
  • [ 6 ] Ibrutinib has demonstrated efficacy as monotherapy for rituximab-resistant cases, especially those with mutations in the MYD88 gene. (medscape.com)
  • WGS was performed on all isolates to detect the presence of genes or mutations previously associated with resistance. (cdc.gov)
  • RT027 isolates also showed higher MICs to clindamycin and moxifloxacin and were more likely to harbor associated resistance genes or mutations. (cdc.gov)
  • The lack of vanA/B genes or mutations consistently associated with elevated vancomycin MICs suggests there are multifactorial mechanisms of resistance. (cdc.gov)
  • Using the DTS method, we have successfully identified an 1812-bp additional GUS gene from the complicated Arabidopsis seedling cDNA library. (nih.gov)
  • As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
  • DSN eliminates nearly all of the common sequences in the tester and driver cDNA samples after the first hybridization step, ensures accurate discrimination between the tester and the driver cDNA samples, and enriches the full-length differential cDNAs from the tester. (nih.gov)
  • To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of polymerase chain reaction (PCR) primer sequences. (frontiersin.org)
  • The poly(A) tailing method indicated that Desulfobacterales were the predominant Deltaproteobacteria, while most of the sequences in libraries constructed using RT-PCR were derived from Desulfuromonadales. (frontiersin.org)
  • A comparison of library diversity indices indicated that the poly(A) tailing method retrieves more phylogenetically diverse sequences from the environment. (frontiersin.org)
  • Considering the current limitations of NGS technology, full-length 16S rRNA gene sequences are better suited to downstream analytical methods such as fluorescence in situ hybridization. (frontiersin.org)
  • The original designs of most of the PCR primers used for the analysis of 16S rRNA genes were based on known sequences deposited in public databases. (frontiersin.org)
  • Mar. 9, 2022 Computational biologists have created a neural network model capable of predicting how changes to non-coding DNA sequences in yeast affect gene expression. (sciencedaily.com)
  • Consequently, established primers specifically amplifying the archaeal 16S ribosomal gene region are scarce compared to the variety of primers targeting bacterial sequences. (frontiersin.org)
  • The RDP Classifier is suitable both for the analysis of single rRNA sequences and for the analysis of libraries of thousands of sequences. (asm.org)
  • The full database consists of 6,076 contigs and 58,221 singletons assembled from both the traditional EST and 454 sequences. (usda.gov)
  • Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (nih.gov)
  • All NTM isolates, representing 183 rapidly growing and 131 slowly growing organisms, were previously identified by Sanger DNA sequencing of the full-length 16S rRNA gene, and region V of the rpoB gene. (cdc.gov)
  • But in this study, published in the American Journal of Infection Control , of the 12 isolates that underwent whole-genome sequencing, 42% of the CA-CRE isolates carried the carbapenemase gene. (medscape.com)
  • The fact that 5 of the 12 isolates harbored a carbapenemase gene introduces new challenges for controlling spread of CP-CRE. (medscape.com)
  • Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (nih.gov)
  • AlbaTraDIS automatically identified all well characterised Triclosan resistance genes, including the primary target, fabI . (biorxiv.org)
  • The Kansas City Public Library hosts author Eugene Halton for his ruminations on the battle between autonomy and automation as presented in his book The Great Brain Suck and Other American Epiphanies on Thursday, January 8 , at 6:30 p.m. at the Central Library , 14 W. 10th St. (kclibrary.org)
  • Free parking is available in the Library District Parking Garage at 10th and Baltimore. (kclibrary.org)
  • METHODS: We combine three complementary approaches by examining Aedes albopictus mosquito gene expression (transcriptomics), bacterial flora (metagenomics) and CHIKV evolutionary dynamics (genomics) induced by viral infection and temperature changes. (bvsalud.org)
  • Mar. 20, 2019 As an organism grows and responds to its environment, genes in its cells are constantly turning on and off, with different patterns of gene expression in different cells. (sciencedaily.com)
  • By studying gene expression patterns of an organism, one can learn how that organism responds to its environment and thereby detect vulnerabilities in its lifecycle which can be exploited to develop control technologies. (usda.gov)
  • Serine/threonine-protein kinase Sgk3 is an enzyme that in humans is encoded by the SGK3 gene. (wikipedia.org)
  • In metabolic engineering, the overexpression of enzyme genes may fail to increase the production yield because cellular resources are excessively consumed for enzyme synthesis, resulting in decreased cell growth and thereby a decreased yield. (biomedcentral.com)
  • In addition, the non-optimized expression of enzyme genes may facilitate the accumulation of toxic intermediate metabolites, which decreases the production yield. (biomedcentral.com)
  • To resolve this problem, promoters with different strengths are widely employed to fine-tune the expression levels of enzyme genes [ 8 , 9 ]. (biomedcentral.com)
  • Find articles, book chapters, journals and books in the holdings of Lund University libraries. (lu.se)
  • If an article is not within the collections of the Lund University Libraries you can make a request for an interlibrary loan. (lu.se)
  • Find all digital and printed journals at Lund University Libraries. (lu.se)
  • This process led to the identification of several gene coding regions with possible roles in sex determination in the developing screwworm embryo. (usda.gov)
  • A recent refinement of this approach allows the roles of essential genes in conditional stress survival to be inferred by altering their expression. (biorxiv.org)
  • This resource, combining a unique new dataset and novel application of analysis methods to multiple relevant datasets, identifies numerous potential drug targets for manipulating circuit function, reveals neuron subtype-specific roles for disease-linked genes, and is useful for understanding gene expression changes observed in human patient brains. (jneurosci.org)
  • A genome-scale library of Gateway-adapted human ORF clones, the CCSB Human ORFeome Library (aka hORFeome V5.1) is ideal for transferring thousands of open reading frames into a variety of protein expression systems. (horizondiscovery.com)
  • Known as the hORFeome v5.1, it contains over 15,000 individual clones representing over 12,000 unique genes. (horizondiscovery.com)
  • These clones are derived from fully-sequenced Mammalian Gene Collection (MGC) full-length cDNAs and subsequently cloned into a Gateway recombinational entry vector. (horizondiscovery.com)
  • Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)
  • Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. (nih.gov)
  • This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. (xenbase.org)
  • Clones containing histone genes were isolated from a library prepared for this purpose. (bl.uk)
  • Microinjection of the major cluster clones in to Xenopus oocytes, confirmed that these genes were functional. (bl.uk)
  • A third of the transcriptome showed similarity with previously characterized immune-related genes that included both the known and putative immune genes. (biomedcentral.com)
  • The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
  • To overcome this problem, we developed a novel method to identify the unique full-length cDNAs in two complicated tissue or cell types. (nih.gov)
  • Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes. (jax.org)
  • J:80000 The FANTOM Consortium and The RIKEN Genome Exploration Research Group Phase I & II Team, Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. (jax.org)
  • Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). (horizondiscovery.com)
  • Follow the symbol links to get more information on the GO terms, expression assays, orthologs, phenotypic alleles, and other information for the genes or markers below. (jax.org)
  • In addition, cell growth and lysine (a precursor of cadaverine) production assays suggested that gene expression optimization through transcription tuning can afford a balance between the growth and precursor supply. (biomedcentral.com)
  • In honor and in celebration of Native American Heritage Month, the City of Eugene Cultural Services and Office of Equity & Community Engagement are pleased to host "Oregon is Indian Country. (eugene-or.gov)
  • The City of Eugene plans are free to use. (eugene-or.gov)
  • As of 2020 that means that the City of Eugene plan (approx. (eugene-or.gov)
  • In cell engineering fields, such as synthetic biology and metabolic engineering, gene expression is fine-tuned for the robust operation of artificially designed genetic systems and enhanced production of a desired substance via manmade metabolic pathways [ 4 , 5 , 6 , 7 ]. (biomedcentral.com)
  • Its genomic and transcriptomic analyses have revealed that it possesses many genes that are involved in methane metabolism and secondary metabolite biosynthetic pathways, including the tricarboxylic acid (TCA) cycle, the ribulose monophosphate (RuMP) cycle, the Embden-Meyerhof-Parnas (EMP) pathway, the pentose phosphate (PP) pathway, the Entener-Doudoroff (EDD) pathway, and the methylerythritol 4-phosphate (MEP) pathway [ 10 , 11 ]. (biomedcentral.com)
  • DH-1 genome also contains a group of genes related to conventional type II methanotrophic metabolic pathways, such as tetrahydromethanopterin and tetrahydrofolate pathways [ 11 ]. (biomedcentral.com)
  • In the present study we constructed and analysed the immune transcriptome of the lepidopteran Antheraea mylitta , an economically important Indian tasar silkmoth with a view to unravel the potential immune-related genes and pathways. (biomedcentral.com)
  • We observe that (i) CHIKV infection upregulated most genes (mainly in immune and stress-related pathways) at 20°C but not at 28°C, (ii) CHIKV infection significantly increased the abundance of Enterobacteriaceae Serratia marcescens at 28°C and (iii) CHIKV evolutionary dynamics were different according to temperature. (bvsalud.org)
  • The Digital Library strives to provide access to its collections and resources to the Villanova University community and the global community of scholars, subject to any intellectual property or other restrictions on use. (villanova.edu)
  • Functional annotation of a full-length mouse cDNA collection. (jax.org)
  • To understand the molecular-genetic basis of functional specialization and identify potential drug targets specific to each neuron subtype, we performed a genome wide assessment of both gene expression and splicing across EXC, PV, SST and VIP neurons from male and female mouse brains. (jneurosci.org)
  • These results reveal numerous examples where neuron subtype-specific gene expression, as well as splice-isoform usage, can explain functional differences between neuron subtypes, including in presynaptic plasticity, postsynaptic receptor function, and synaptic connectivity specification. (jneurosci.org)
  • Microinjection analysis again showed the genes were functional. (bl.uk)
  • The organization of the SELPLG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters. (wikidoc.org)
  • Through three different approaches, we constructed a tunable promoter library comprising 33 promoters that can be used for the metabolic engineering of Methylomonas sp. (biomedcentral.com)
  • Numerous studies on natural microbial communities from a variety of environments have been undertaken using 16S rRNA gene sequencing mediated by polymerase chain reaction (PCR) with oligonucleotide primers. (frontiersin.org)
  • Finally, we analysed the expression profiles of 15 potential immune-related genes, and the majority of them were induced more prominently with E. coli compared to Micrococcus luteus . (biomedcentral.com)
  • With reference to insect immunity, the ESTs have been obtained from baculovirus-infected B. mori cultured cells and pupae, but no large scale information on bacteria-induced immune genes is as yet available. (biomedcentral.com)
  • Prior information on immune response genes in wild silkmoths is lacking except for a few peripheral studies. (biomedcentral.com)
  • We also validated the upregulation of a selected subset of genes from the immune transcriptome by semi-quantitative RT-PCR. (biomedcentral.com)
  • Liu Y, Zhang YZ, Imoto S . Microbial Gene Ontology informed deep neural network for microbe functionality discovery in human diseases. (google.com)
  • Furthermore, in-silico evaluation of primers, especially those for amplification of archaeal 16S rRNA gene regions, does not necessarily reflect the results obtained in experimental approaches. (frontiersin.org)
  • AMR, through acquired genes located on transposons or conjugative plasmids, is the horizontal transmission of genes required for a given bacteria to withstand antibiotics. (mdpi.com)
  • The genes for this are readily transferable between bacteria and help account for their spread in hospitals. (medscape.com)
  • Search across multiple databases and the library catalog simultaneously. (lu.se)
  • For practical application of the promoter library, we fine-tuned the expressions of cadA and cadB genes, required for cadaverine synthesis and export, respectively. (biomedcentral.com)
  • The work of Eugene artist Jimmy Prahl, saturated with ink, paint and markers and containing a digital slant, is on display at WOW Hall through December. (eugeneweekly.com)
  • This gene is a member of the serine/threonine protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. (wikipedia.org)
  • Interferon gamma receptor 1 ( IFNGR1 ) also known as CD119 ( C luster of D ifferentiation 119 ), is a protein that in humans is encoded by the IFNGR1 gene . (wikidoc.org)
  • The genes provide instructions for making the MTHFR protein. (medlineplus.gov)
  • The gene IFNGR1 encodes IFN-γR1, which is the ligand-binding chain (alpha) of the heterodimeric gamma interferon receptor, which is found on macrophages . (wikidoc.org)
  • Loss of Tada2b or Taf5l inhibited hematopoiesis in vivo and was associated with upregulation of interferon response gene expression. (biorxiv.org)
  • The picture of histone gene cluster structure in X. borealis that emerges from these studies, is compared at the nucleotide, gene, and chromosomal levels with X. laevis. (bl.uk)
  • This database will facilitate the design of experiments to study screwworm gene expression on a larger scale than previously possible. (usda.gov)
  • These Gateway-adapted ORFs are ideal for easily moving gene content into compatible destination vectors for various proteomics studies. (horizondiscovery.com)
  • Psychoactive medications in water affect the gene expression profiles of fathead minnows in a way that mimics the gene expression patterns associated with autism spectrum disorder in genetically susceptible humans, according to new research. (sciencedaily.com)
  • Psychoactive medications in water affect the gene expression profiles of fathead minnows in a way that mimics the gene expression patterns associated with autism spectrum disorder in genetically susceptible humans, according to research published June 6 in the open access journal PLoS ONE . (sciencedaily.com)
  • They found that the only gene expression patterns affected were those associated with idiopathic autism spectrum disorders, caused by genetic susceptibility interacting with unknown environmental triggers. (sciencedaily.com)
  • This rate is patterns, gene sequencing, immuno- Centre for Disease Prevention and higher than that of severe acute respira- genicity and the animal-human and hu- Control (ECDC) and the National In- tory syndrome coronavirus (SARS), man-human interfaces. (who.int)
  • Documents regarding divorce from Kathleen Pitt-Smith, and child support for Eugene O'Neill, Jr. (yale.edu)
  • Researchers around the world are achieving targeted gene silencing in cells that had previously been beyond the reach of conventional RNAi products due to toxicity caused by transfection reagents or undesirable viral responses. (horizondiscovery.com)
  • Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum . (nature.com)
  • Two normalized cDNA libraries were constructed from RNA isolated from embryos and 2nd instar larvae from the Panama 95 strain. (usda.gov)
  • In our study we have used olf413 gene disruption strain, olf413MI02014 homozygous and in transheterozygous condition with another allele isolated in our lab, olf413SG1.1. (who.int)
  • Additionally, the identification of sex determination genes in the screwworm will assist in the development of a control technology based on transgenic strains of screwworm which produce only males, a technology which would be applicable to the USDA-APHIS screwworm eradication program's production facilities. (usda.gov)
  • Park H, Imoto S , Miyano S. Comprehensive information-based differential gene regulatory networks analysis (CIdrgn): Application to gastric cancer and chemotherapy-responsive gene network identification. (google.com)
  • Eugene Public Library "Connections," quilts by members of Tactile Expressions, through Dec. 31. (eugeneweekly.com)
  • Your reference library headers must also contain unique substrings associated with each group. (cdc.gov)
  • Overall our results indicate a rather limited comparability between community structures investigated and determined using different primer pairs as well as between metagenome and 16S rRNA gene amplicon based community structure analysis. (frontiersin.org)
  • a software application for rapid large-scale comparative analysis of TraDIS experiments that predicts the impact of transposon insertions on nearby genes. (biorxiv.org)
  • This prompted more detailed analysis of histone gene arrangements in X. borealis, which is the subject of this thesis. (bl.uk)
  • AlbaTraDIS can identify genes which are up or down regulated, or inactivated, between multiple conditions, producing a filtered list of genes for further experimental validation as well as several accompanying data visualisations. (biorxiv.org)