Among the genes that build HMGs in the mammary gland is the fucosyltransferase 2 (FUT2) gene, which catalyzes the transfer of fucose residues by an α1,2-linkage to glycans found in human milk. (biomedcentral.com)
Glycosylation of Notch receptors by O-fucose glycans regulates Notch ligand binding and Notch signaling during hematopoiesis. (bvsalud.org)
The combined results show that EOGT and O-GlcNAc glycans are required for optimal hematopoiesis and T and B cell development, and that they act synergistically with POFUT1 and O-fucose glycans to promote Notch signaling in lymphoid and myeloid differentiation. (bvsalud.org)
Although lectins were already known because of their ability to agglutinate red blood cells, 3 the involvement of lectins and glycans in PCD had not been elucidated. (nature.com)
Galactoside1
Galactoside 3(4)-L-fucosyltransferase is an enzyme that in humans is encoded by the FUT3 gene. (wikipedia.org)
Secretor6
The synthesis of Lewis antigens depends on the interaction of the transferases produced by the Lewis and secretor genes. (keepnotes.com)
The Lewis and secretor transferases preferentially fucosylate type 1 chains, whereas the H gene (FUT1) preferentially fucosylates type 2 chains. (keepnotes.com)
o The Lewis fucosyltransferase (FuT 3) is more active than the secretor fucosyltransferase (FuT 2) in newborns, so more type 1 chains are available for conversion to Lea. (keepnotes.com)
In secretor-fed infants, the relative abundance of the Bifidobacterium longum group was most strongly correlated with high percentages of the order Bifidobacteriale s. (biomedcentral.com)
Conversely, in non-secretor-fed infants, Bifidobacterium breve was positively correlated with Bifidobacteriale s, while the B. longum group was negatively correlated. (biomedcentral.com)
Known as the 'secretor' gene because of its role in the expression of ABO blood types in various secreted body fluids (tears, saliva, breast milk, and so on), this gene has well-known mutations that inactivate transferase activity which occur in most populations across the world, including in about 20% of the population of the United States [ 21 ]. (biomedcentral.com)
Precursor2
This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. (wikipedia.org)
The key signaling pathways in regulating neurogenesis and neural precursor cells properties, transforming growth factor-ß (TGF-ß) and its downstream signaling pathways were further examined to clarify the potential mechanism of L-Fucose on the injured ENS and ENPCs. (bvsalud.org)
Chromosome 191
The Lewis gene (FUT3) is linked to Se (FUT2) and H (FUT1), all located on chromosome 19. (keepnotes.com)
Phenotype2
They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. (wikipedia.org)
o As a result, cord blood and RBCs from newborn infants phenotype as Le(a -b-). o Some can be shown to be weakly Le(a+) when tested with a potent anti-Lea or with methods more sensitive than direct agglutination. (keepnotes.com)
ELISA2
Antibodies that detect Blood Group Lewis A can be used in several scientific applications, including Immunohistochemistry, Immunocytochemistry, Flow Cytometry, ELISA and SDS-PAGE. (thermofisher.com)
We also performed in vitro experiments with Sparstolonin B and detected changes in 1,3-fucosyltransferase activity by ELISA. (hindawi.com)
Genes3
Expression of human chromosome 19p alpha(1,3)-fucosyltransferase genes in normal tissues. (wikipedia.org)
Development of Lewis antigens o Depending on the genes inherited, Lea and Leb glycoproteins will be present in the saliva of ewborns, but Lewis glycolipids are not detectable in the plasma until about 10 days after birth. (keepnotes.com)
Genome-wide comparison between healthy nonsmokers (n = 60) grouped as "high MUC5AC expressors" vs "low MUC5AC expressors" identified 528 genes significantly up-regulated and 15 genes significantly down-regulated in the high vs low expressors. (biomedcentral.com)
Gene3
Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. (wikipedia.org)
Isolation of a novel human alpha (1,3)fucosyltransferase gene and molecular comparison to the human Lewis blood group alpha (1,3/1,4)fucosyltransferase gene. (wikipedia.org)
o Lewis antigens will start to appear shortly after birth, with Lea developing first when the Le gene is present. (keepnotes.com)
Enzyme1
It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. (wikipedia.org)
Bacterial1
Fucose was added to the bacterial culture medium to further study its effects on metabolism. (bvsalud.org)
Monoclonal2
Our Blood Group Lewis A monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. (thermofisher.com)
Murine leukocytes are thought to express α2-3-sialylated and α1-3-fucosylated selectin ligands such as sialyl Lewis x (sLe x ), although monoclonal antibodies (mAbs) to sLe x or Le x reportedly do not bind to murine leukocytes. (ashpublications.org)
Antibody1
These antibodies have been verified by Relative expression to confirm specificity to Blood Group Lewis A. Find the Blood Group Lewis A antibody that fits your needs. (thermofisher.com)
Development1
To investigate the effects of fucose on ISCs-mediated intestinal epithelial cells (IECs) development, we administered fucose to 4-week-old mice for 4 weeks. (bvsalud.org)
Human2
These antibodies target Blood Group Lewis A in Human, Mouse samples. (thermofisher.com)
Compared to human promyelocytic HL-60 cells, WEHI-3 cells and murine neutrophils expressed low α1-3-fucosyltransferase activities. (ashpublications.org)
Transfer1
Measured by its ability to transfer fucose from GDP-fucose to N -Acetyllactosamine The specific activity is >35 pmol/min/µg, as measured under the described conditions. (rndsystems.com)
Treatment3
The decrease in the number of neurons and glial cells in the ENS of diabetic mice were reversed by L-Fucose treatment. (bvsalud.org)
More importantly, L-Fucose treatment significantly promoted the proportion of ENPCs differentiated into neurons and glial cells both in vitro and in vivo, accompanied by inhibiting SMAD2 phosphorylation. (bvsalud.org)
The composition and functions of gut bacteria were altered by fucose treatment, while significant increases in Akkermansia and propanoate metabolism were noted. (bvsalud.org)
Results3
RESULTS: L-Fucose improved gastrointestinal motility in diabetic mice, including increased defecation frequency (p (bvsalud.org)
The results showed that fucose accelerated ISCs proliferation and secretory lineage differentiation in mice, whereas antibiotics eliminated these effects. (bvsalud.org)
In Japan, many studies on complex carbohydrates have long been accomplished by members of the research groups supported by the Ministry of Education, Science, Sports and Culture (MESSC), and excellent results have been produced. (gr.jp)
Cells2
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. (wikipedia.org)
Consistent with very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells or murine neutrophils by exogenous α1-3-fucosyltransferase FTVI and GDP-fucose created many new epitopes for anti-sLe x mAbs such as HECA-452 and CSLEX-1. (ashpublications.org)
Role1
To explore the role of α 1,3-fucosyltransferase in the mediation of rheumatoid arthritic inflammation, the protective effect of Sparstolonin B on rheumatoid arthritis (RA), and the mechanisms that regulate the NLRP3 inflammasome. (hindawi.com)
High1
Using diabetic mice in vivo and high glucose-treated primary ENPCs in vitro, the effects of L-Fucose on the injured ENS and ENPCs was evaluated by assessing gastrointestinal motility, ENS structure, and the differentiation of ENPCs. (bvsalud.org)
Type1
CONCLUSIONS: L-Fucose could promote neurogenesis and gliogenesis derived from ENPCs by inhibiting the SMAD2 signaling, thus facilitating ENS regeneration and gastrointestinal motility recovery in type 1 diabetic mice. (bvsalud.org)
Production1
These two groups are both involved in the production of short-chain fatty acids and lactate, which alter the pH of the environment, modulate the microbiota, and have other systemic properties [ 19 ]. (biomedcentral.com)
Addition of fucose2
This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. (wikipedia.org)
They can be further modified by the addition of fucose or sialic acid residues at the terminal positions and are joined by a multitude of linkages. (biomedcentral.com)
Biosynthesis2
identification of the sialylation sequence and the effects of sulfate, fucose, methyl, and fluoro substituents of the carbohydrate chain in the biosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha2,3(O)sialyltransferase. (nih.gov)
Fucose: biosynthesis and biological function in mammals. (research.com)
Antigens5
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. (nih.gov)
The presence of different ABH and Lewis antigens in different tissues and the different epistatic interactions needed to account for their synthesis are analysed in terms of new epistatic interactions with either the known fucosyltransferases or with new fucosyltransferases. (nih.gov)
ABH and related histo-blood group antigens in normal & malignant human endometrium in relation to genetic and hormonal factors. (nih.gov)
Using EL4 cell-derived transfectants with differential expression levels of exogenous human CC chemokine receptor 4 or human CD20 as target cells, ADCC of fucose variants of chimeric IgG1 antibodies specific for these antigens were measured. (aacrjournals.org)
We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLe(x)) and Lewis y (Le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltra. (shengsci.com)
Histo-blood1
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. (wikipedia.org)
Antigen10
Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. (wikipedia.org)
In this report, we investigated the relationship between enhanced ADCC and antigen density on target cells using IgG1 antibodies with reduced fucose. (aacrjournals.org)
We further investigated IgG1 binding to natural killer (NK) cells and NK cell activation during ADCC induction to elucidate the mechanism by which low-fucose IgG1 induces ADCC upon target cells with low antigen expression. (aacrjournals.org)
Low-fucose IgG1s showed potent ADCC at low antigen densities at which their corresponding high-fucose counterparts could not induce measurable ADCC. (aacrjournals.org)
The quantitative analysis revealed that fucose depletion could reduce the antigen amount on target cells required for constant degrees of ADCC induction by 10-fold for CC chemokine receptor 4 and 3-fold for CD20. (aacrjournals.org)
IgG1 binding to NK cells was increased by ligating IgG1 with clustered antigen, especially for low-fucose IgG1. (aacrjournals.org)
Our data showed that fucose removal from IgG1 could reduce the antigen amount required for ADCC induction via efficient recruitment and activation of NK cells. (aacrjournals.org)
Cloning, expression and identification of a gene CgFUT5 associated with Lewis antigen synthesis of Oyster norovirus receptors[J]. South China Fisheries Science. (schinafish.cn)
Lewis antigen is regarded as a specific binding receptor for norovirus, and Lewis-like antigen is also present in oysters as a vehicle for norovirus transmission, but the pathway for synthesis of this carbohydrate in oysters has not been elucidated. (schinafish.cn)
To clarify the pathway of norovirus receptor-like Lewis antigen synthesis in oysters, we cloned the CgFUT5 gene from the Pacific oyster ( Crassostrea gigas ) genome and analyzed it for expression in five tissues. (schinafish.cn)
Molecular2
Isolation of a novel human alpha (1,3)fucosyltransferase gene and molecular comparison to the human Lewis blood group alpha (1,3/1,4)fucosyltransferase gene. (wikipedia.org)
12. Human lung adenocarcinoma alpha1,3/4-L-fucosyltransferase displays two molecular forms, high substrate affinity for clustered sialyl LacNAc type 1 units as well as mucin core 2 sialyl LacNAc type 2 unit and novel alpha1,2-L-fucosylating activity. (nih.gov)
Sialyl4
Myeloid type of alpha-3-fucosyltransferase, which transfers fucose on to H type 2, serum type of alpha-3-fucosyltransferase, which transfers fucose on to H type 2 and sialyl-N-acetyl-lactosamine and Lewis or alpha-3/4-fucosyltransferase, which transfers fucose on to H type 1 and H type 2 and the corresponding sialylated structures. (nih.gov)
8. Selectin ligands and tumor-associated carbohydrate structures: specificities of alpha 2,3-sialyltransferases in the assembly of 3'-sialyl-6-sialyl/sulfo Lewis a and x, 3'-sialyl-6'-sulfo Lewis x, and 3'-sialyl-6-sialyl/sulfo blood group T-hapten. (nih.gov)
His study explores the link between Fucosyltransferase and topics such as Sialyl-Lewis X that cross with problems in Cell adhesion. (research.com)
His work investigates the relationship between Fucosyltransferase and topics such as Sialyl-Lewis X that intersect with problems in Glycosylation and High endothelial venules. (research.com)
Serum1
Its larger kit size is ideal for high-throughput automated workflows used for the analysis of biological samples such as blood serum or plasma (see our workflow below). (ludger.com)
Alpha1
Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha(1,3)-fucose and core xylose. (shengsci.com)
Immune system4
In the subject of general Immunology, his work in Immune system, Chemokine and Lymphocyte homing receptor is often linked to Cell fate determination, thereby combining diverse domains of study. (research.com)
Goblet cells dispersed among the intestinal epithelial cells are chiefly responsible for the synthesis and secretion of mucins within the gut and are heavily influenced by interactions with the immune system. (frontiersin.org)
As the bridge between the internal and external environments, it is no surprise then that roughly 70% of the immune system resides within the GI tract ( 1 ). (frontiersin.org)
Rhesus macaques, Macaca mulatta (Mm) share approximately 93% sequence identity with humans but display important differences in their adaptive immune system that complicates their use in validating therapeutics and vaccines that rely on Fc effector functions. (bvsalud.org)
Evidence1
6. Evidence for an O-glycan sialylation system in brain. (nih.gov)
Analysis2
Classes for this protein according to Protein ANalysis THrough Evolutionary Relationships (PANTHER) Classification System. (nih.gov)
Ludger V-Tag system is designed for the analysis of glycopeptides generated from the digestion of therapeutic glycoproteins such as monoclonal antibodies (mAb) . (ludger.com)
Presence1
Up-regulation of an activation marker, CD69, on NK cells, particularly the CD56 dim subset, in the presence of both the antibody and target cells was much greater for the low-fucose antibodies. (aacrjournals.org)