Cycloheximide exerts its effects by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking eukaryotic translational elongation. (wikipedia.org)
Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). (wikipedia.org)
Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. (wikipedia.org)
Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein of interest can show differences in protein half-life. (wikipedia.org)
Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation. (wikipedia.org)
Cycloheximide is a protein synthesis inhibitor in eukaryotic cells. (goldbio.com)
IL-1 beta also induces the accumulation of cGMP by the insulinoma cell line Rin-m5F, and both NMMA as well as the protein synthesis inhibitor cycloheximide prevent this cGMP accumulation. (jci.org)
Nuclear movement and flagellar root contraction and extension are not sensitive to inhibitors of protein synthesis (cycloheximide), or drugs that influence either microtubules (colchicine) or actin-based microfilaments (cytochalasin D). Detergent-extracted cell models contract and extend their flagellar roots and move their nuclei in response to alterations of free calcium levels in the medium. (rupress.org)
The effect of arachidonic acid on GluR1,3 currents was not observed in Ca2+-free extracellular solution, and the potentiation was blocked by either KN-93, a selective Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor, or NP217, an active CaMKII inhibitor peptide, when co-expressed with the receptors. (msu.ru)
Furthermore, studies using cycloheximide indicate that these GH-induced changes in mRNA expression do not require protein synthesis and hence are primary, not secondary events. (aacc.org)
Bacteria2
Cycloheximide has also been used to make isolation of bacteria from environmental samples easier. (wikipedia.org)
Cellulose breakdown was only partially inhibited in the presence of cycloheximide, suggesting that bacteria participated in this process. (mpg.de)
Elongation2
The translational elongation freezing properties of cycloheximide are also used for ribosome profiling / translational profiling. (wikipedia.org)
Polysome fractionation begins with immobilizing ribosomes on mRNAs using inhibitors of translation elongation, for example, cycloheximide. (bvsalud.org)
Fungal1
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Amino acids2
Cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of Neurospora crassa" (PDF). (wikipedia.org)
In contrast to the influx of amino acids, the degradation of proteins is also the important intracellular mechanism for releasing free amino acids both under steady-state conditions and during cellular stresses. (spandidos-publications.com)
In general, GoldBio's Molecular Grade cycloheximide is suitable for most experiments. (goldbio.com)
Our experiments show that cyanobacterial cultures are easily contaminated by sterol-producing rust fungi, which can be eliminated by treatment with cycloheximide affording sterol-free samples. (wegrow.live)
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Dermatophytes1
Cycloheximide has been used to isolate dermatophytes and inhibit the growth of fungi in brewing test media. (wikipedia.org)
Impurities2
Cyclohex99 should be used for the most sensitive applications as it is free of impurities. (goldbio.com)
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Vitro2
Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. (wikipedia.org)
In vitro treatment with TIMP-1 inhibited cycloheximide-induced cell death of primary mouse hepatocytes. (biomedcentral.com)
Fungi1
The following represents susceptibility data for a few commonly targeted fungi: Candida albicans: 12.5 μg/ml Mycosphaerella graminicola: 47.2 μg/ml - 85.4 μg/ml Saccharomyces cerevisiae: 0.05 μg/ml - 1.6 μg/ml Neoscytalidium dimidiatum is an Athlete's foot like infection resistant to most antifungals but is rather sensitive to cycloheximide, so, it should be cultured in a medium free of cycloheximide. (wikipedia.org)
Rapidly1
Because cycloheximide rapidly breaks down in a basic environment, decontamination of work surfaces and containers can be achieved by washing with a non-harmful alkali solution such as soapy water or aqueous sodium bicarbonate. (wikipedia.org)
Cellular1
Cycloheximide can be used to study cellular processes and inhibit yeast growth. (goldbio.com)
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Translation1
Translation is halted via the addition of cycloheximide, and the DNA/RNA in the cell is then nuclease treated. (wikipedia.org)
Medium1
Anterior displacement of the nucleus and flagellar root contraction require free calcium in the medium. (rupress.org)
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Cells1
It can be grown on any of these cell lines treated with cycloheximide: McCoy, HEp-2, HeLa, or buffalo green monkey cells. (medscape.com)
Cell Death2
Anti-apoptotic actions of cycloheximide: Blockade of programmed cell death or induction of programmed cell life? (johnshopkins.edu)
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ACCESS1
The journal initiated the Clinical Chemistry Trainee Council to give doctoral level trainees in 25 countries free online access to instructional materials, including the launch of a Spanish-language version. (aacc.org)
Research1
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Disease-free1
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Action1
The purpose of this investigation was to determine if the beta-cell, which is selectively destroyed during insulin-dependent diabetes mellitus, is both a source of IL-1 beta-induced nitric oxide production and also a site of action of this free radical. (jci.org)