GlycoproteinsPeptidesSynthesisPeptide fragmentsAntibodyFragmentationAntibodiesGlycan fragmentsMucinAntibioticScFvCharacterizationGlycansVancomycinAntigenSyntheticGenesCollisionSpectrometrySequencesExperimentDetectionIdentificationBacterialMassInstrumentShortVarietyEssentialAchieveMethodTypeHighPotential
Glycoproteins1
- Moreover, our lab has developed techniques to detect glycoproteins and glycopeptides across digestion. (oregonstate.edu)
Peptides3
- Using the convergent glycopeptide synthesis it is possible to synthesize libraries of homogeneously glycosylated peptides. (uni-bayreuth.de)
- In particular, I am interested in bioactive milk proteins and fragments of proteins-peptides-released within the mammary gland and during digestion. (oregonstate.edu)
- Zylacta has developed and patented a biochemical platform for generating a vast variety of new biologically active glycopeptides and D-amino acids containing peptides from bacterial cellular components. (zylacta.com)
Synthesis3
- Chemical and chemoenzymatic synthesis of glycosyl-amino acids and glycopeptides related to Trypanosoma cruzi mucins. (ox.ac.uk)
- In this context, this work describes the synthesis of the hypoglycosylated alpha-DG mimetic glycopeptide NHAc-Gly-Pro-Thr-Val-Thr[alphaMan]-Ile-Arg-Gly-BSA (1) as a potential tool for the development of novel antibodies applicable to dystroglycanopathies diagnosis. (cnr.it)
- On-chip neo-glycopeptide synthesis for multivalent glycan presentation. (mpg.de)
Peptide fragments3
- 1,2 discussed a new glycopeptide instant expertise method which makes use of multiple collision energies to produce glycan fragments and peptide fragments inside the same MS/MS spectrum. (news-medical.net)
- 2. Henzel WJ, Billeci TM, Stults, JT, Wong SC, Grimley C and Watanabe C: Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases. (toddbilleci.com)
- 4. Henzel WJ, Billeci TM, Stults JT, Wong S, Grimley C and Watanabe C: Identifying proteins at the femtomole level by molecular mass searching of peptide fragments in a protein sequence database. (toddbilleci.com)
Antibody3
- The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. (wikipedia.org)
- Fcab fragments can be inserted into a full immunoglobulin by swapping the Fc region, thus obtaining a bispecific antibody (with both Fab and Fcab regions containing distinct binding sites). (wikipedia.org)
- After affinity isolation on immobilized BC3 antibody, MUC1 was partially deglycosylated by enzymatic treatment with alpha-sialidase/beta-galactosidase and fragmented by proteolytic cleavage with the Arg-C-specific endopeptidase clostripain. (nih.gov)
Fragmentation5
- During an MS/MS experiment, glycopeptides may not be selected for fragmentation, or may not fragment. (labnews.co.uk)
- It is possible to achieve glycopeptide fragmentation under standard collision induced dissociation (CID) conditions, in which the glycosidic bonds between carbohydrate bonds are favourably cleaved. (news-medical.net)
- To find a way around this issue, deeper investigation of the fragmentation of N-glycopeptides was carried out using Bruker oTOF instruments. (news-medical.net)
- In the case of N-glycopeptide fragmentation, the higher collision energies, which are required for peptide moiety fragmentation, also bring about a characteristic fragmentation pattern, which has earlier been described for MALDI fragmentation 3 . (news-medical.net)
- News in Proteomics Research: Optimal Fragmentation of N- and O- Glycopeptides! (blogspot.com)
Antibodies3
- Glycopeptide 1 was used for the development of polyclonal antibodies and recombinant monoclonal antibodies by Phage Display technology. (cnr.it)
- Accordingly, polyclonal antibodies were reactive to glycopeptide 1, which enables the application of anti-glycopeptide 1 antibodies in immune reactive assays targeting hypoglycosylated alpha-DG. (cnr.it)
- Along with his colleagues, he also studied antibodies against SARS S protein fragments to identify potential vaccines. (keepyourinnerarmystrong.com)
Glycan fragments2
- Whilst most approaches with ion mobility are focused on intact glycans, we dedicated this study to glycan fragments. (a-star.edu.sg)
- Based on the collision cross section of glycan fragments, we can individually identify previously indistinguishable glycan isomers such as bisecting N-acetylglucosamine and tri-antennary glycans, galactose glycan arm extension and sialic acid extension, among others. (a-star.edu.sg)
Mucin1
- Using this knowledge, short glycopeptide fragments based on T. cruzi mucin sequences, Thr-Thr-[LacNAcThr]-Thr-Thr-Gly, were synthesised. (ox.ac.uk)
Antibiotic2
- Glycopeptide antibiotic complex from Actinoplanes teichomyceticus active against gram-positive bacteria . (lookformedical.com)
- The wrapping method is that the distance a gel for etching [22] table 4.9 glycerated gelatine suppositories [22] phosphoric acid ester of clinda- glycopeptide antibiotic vanconsycin (vancocin. (themauimiracle.org)
ScFv3
- The best-characterized VH and VL domains were cloned, expressed in E. coli Shuffle T7 cells, and used to construct a single chain fragment variable that recognized the Glycopeptide 1 (GpalphaDG1 scFv). (cnr.it)
- Molecular modelling of glycopeptide 1 and GpalphaDG1 scFv suggested that their interaction occurs through hydrogen bonds and hydrophobic contacts involving amino acids from scFv (I51, Y33, S229, Y235, and P233) and R8 and alpha-mannose from Glycopeptide 1. (cnr.it)
- performed a primary comparison from the in vitro level of sensitivity of each technique, using the same anti-cancer scFv fragments, aimed against a tumor-specific glycopeptide epitope Atractylodin for the sialomucin-like transmembrane glycoprotein OTS8, which outcomes type a cancer-specific mutation of Cosmc. (techieindex.net)
Characterization2
- For glycopeptide characterization, mass spectrometry has arisen as the go-to methodology. (news-medical.net)
- We are showcasing GlycanExplorer ™ , a software tool for high-throughput characterization of bio-therapeutic N -glycans using built-in robust in-silico fragment databases and HRAM glycan spectral databases. (premierbiosoft.com)
Glycans4
- Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions. (fu-berlin.de)
- In this study, we demonstrated that ion-mobility mass spectrometry can distinguish structural differences in similar glycans, by breaking the glycans into smaller fragments and then measuring their shape. (a-star.edu.sg)
- Pallister EG, Choo MSF, Walsh I, Tai JN, Tay SJ, Yang YS, Ng SK, Rudd PM, Flitsch SL, Nguyen-Khuong T. Utility of Ion-Mobility Spectrometry for Deducing Branching of Multiply Charged Glycans and Glycopeptides in a High-Throughput Positive ion LC-FLR-IMS-MS Workflow. (a-star.edu.sg)
- If you are working on released glycans ( N -linked or O -linked) or glycopeptides and are facing analytical challenges, our representative will be happy to discuss this in person during the conference. (premierbiosoft.com)
Vancomycin1
- Avoparcin, a glycopeptide antimicrobial agent related ed glycopeptide antimicrobial agent, has been widely used to vancomycin, has been used extensively as a growth pro- in Europe and other continents as an animal growth pro- moter in animal feeds for more than 2 decades, and evi- moter (Figure 1). (cdc.gov)
Antigen3
- This type of antigen-binding fragment is called Fcab. (wikipedia.org)
- The fragment contains tumour-associated carbohydrate antigen (TACA), Sialyl Tn or STn (Neu5Acα2-6GalNAcα-O-Ser) at Ser130 and Neu5Acα2-6GalNAcα-O-Thr at Thr131. (sussex-research.com)
- The fragment contains tumour-associated carbohydrate antigen (TACA), Sialyl Tn or STn (Neu5Acα2-6GalNAcα- O -Ser) at Ser140 and Neu5Acα2-6GalNAcα- O -Thr at Thr139. (sussex-research.com)
Synthetic1
- 1,2 employed synthetic, and therefore well-defined, N-glycopeptides for the optimization of CID energy parameters, so as to attain the greatest possible quantities of data on both the glycan and peptide moiety within just one tandem MS experiment. (news-medical.net)
Genes2
- A cluster and humans coming into contact with the animals (farm that includes three genes, vanH , vanA , and vanX , is required for high-level resistance to glycopeptides. (cdc.gov)
- Furthermore, nucleotide sequences related to the cluster vanHAX are present in this DNA, suggesting that the prolonged use of avoparcin in agriculture led to the uptake of glycopeptide resistance genes by animal commensal bacteria, which were subsequently transferred to humans. (cdc.gov)
Collision1
- Collision energy stepping implemented in the Glycopeptide Instant Expertise™ method. (news-medical.net)
Spectrometry3
- The PAP20 glycopeptides were isolated by reversed phase high pressure liquid chromatography and subjected to the structural analyses by quadrupole time-of-flight electrospray ionization mass spectrometry and to the sequencing by Edman degradation. (nih.gov)
- Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. (fu-berlin.de)
- By using mass spectrometry approaches, the researchers were able to determine site-specific differences in the IgG glycopeptide. (newsstatix.com)
Sequences2
- Establishment of transgenic fly lines in which the GFP reporter gene was placed under the control of 1.5 kb of metchnikowin gene upstream sequences indicates that this fragment is able to confer full immune inducibility and tissue specificity of expression on the transgene. (cnrs.fr)
- Primers 16S 440F and 16S 1491R (Table) were ing of glycopeptide antimicrobial agents to their designed to amplify partial 16S rDNA sequences. (cdc.gov)
Experiment1
- Additionally, we applied multiple digestion methods on each sample, and confirmed the results for individual glycosylation sites from different experiment conditions to improve the identification and quantification of glycopeptides. (cdc.gov)
Detection1
- Until recently, classification of N-glycopeptide spectra in ProteinScape (detection of glycopeptide spectra and calculation of glycan and peptide moiety mass) has centered on glycan fragment distances and oxonium ion detection when using typical CID conditions on ion trap or qTof instruments (see application note LCMS-66)4. (news-medical.net)
Identification5
- As well as the instrumental arrangement, a suitable software solution is essential to enable glycopeptide identification and cut laborious processing and de novo analysis time. (news-medical.net)
- GlycoQuest (search algorithm in ProteinScape) is Bruker's proven solution and delivers dependable results with regards to glycopeptide identification. (news-medical.net)
- Compound classification and ensuing glycopeptide identification is executed with ProteinScape 4.0 and the embedded search algorithm GlycoQuest. (news-medical.net)
- They handily fragment well right at the peptide bond, but these authors show what appears to be a huge difference in N-glycopeptide identification with an HCD change from 30 to 35 (!yikes! (blogspot.com)
- Identification of multiple druggable secondary sites by fragment screening against DC-SIGN. (mpg.de)
Bacterial1
- It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear. (lookformedical.com)
Mass1
- Despite the fact that the classification workflow in ProteinScape has an explicit parameter to address this issue, the peptide mass of low abundant N-glycopeptides can be miscalculated at times, which can result in no or incorrect identifications. (news-medical.net)
Instrument2
- You might want to verify that your instrument(s) are really doing what they say they're doing if you're thinking about intact glycopeptide work! (blogspot.com)
- It would suck to optimize on your Fusion 1 and then move that to another instrument and waste all those glycopeptide runs! (blogspot.com)
Short1
- We decided to generate and then analyse short glycopeptides as a means of gaining positional as well as structural information. (labnews.co.uk)
Variety1
- Any variety of glycopeptide sample can be employed in this workflow - low complex and high complex, with and without glycopeptide enrichment. (news-medical.net)
Essential1
- Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. (wikipedia.org)
Achieve1
- They achieve this by binding to the fragment crystallizable (Fc) region of IgG, preventing opsonization and phagocytosis of bacteria. (newsstatix.com)
Method1
- Typical glycopeptide CID spectrum achieved by using the Glycopeptide Instant Expertise™ method. (news-medical.net)
High1
- High-level glycopeptide resistance is conferred by a nol-chloroform extractions. (cdc.gov)
Potential1
- We discovered that certain peptidoglycan fragments represent important new classes of potential drugs, which Zylacta is uniquely positioned to develop. (zylacta.com)