• nucleic acids
  • While the type of biomolecule used can vary widely, biosensors can be classified according to common types bioreceptor interactions involving: anitbody/antigen, enzymes/ligands, nucleic acids/DNA, cellular structures/cells, or biomimetic materials. (wikipedia.org)
  • complementarity determi
  • This domain has a structure similar to antibody variable domains, with seven beta sheets forming a beta-sandwich and three exposed loops on each side corresponding to the three complementarity determining regions. (wikipedia.org)
  • scaffold
  • Lately, amino acids outside of the binding surface have been substituted in the scaffold to create a surface entirely different from the ancestral protein A domain. (wikipedia.org)
  • The native FN3 scaffold consists of 94 amino acids and has a molecular mass of about 10 kDa, fifteen times smaller than an IgG type antibody and comparable to the size of a single variable domain of an antibody. (wikipedia.org)
  • define
  • Glycan arrays, like that offered by the Consortium for Functional Glycomics and Z Biotech LLC, contain carbohydrate compounds that can be screened with lectins or antibodies to define carbohydrate specificity and identify ligands. (wikipedia.org)
  • Depending upon the requirements of the investigation, there are several techniques available to define a DNA-recognition domain that will confer the specificity of a ZFP-based transcription factor. (wikipedia.org)
  • IgG1
  • Phosphate-buffered saline and human IgG1 antibodies against fluorescein isothiocyanate were used as controls. (ahajournals.org)
  • One of the IgG1 antibodies significantly and dose-dependently reduced the extent of atherosclerosis as well as the plaque content of oxidized LDL epitopes and macrophages. (ahajournals.org)
  • Gene
  • The large and diverse population of antibody paratope is generated by random recombination events of a set of gene segments that encode different antigen-binding sites (or paratopes), followed by random mutations in this area of the antibody gene, which create further diversity. (wikipedia.org)
  • Subsequent cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). (wikipedia.org)
  • specific
  • However the dissimilarity between murine and human immune systems led to the clinical failure of these antibodies, except in some specific circumstances. (wikipedia.org)
  • Type-specific immunogenicity of a chemically synthesized peptide fragment of type 5 streptococcal M protein. (rupress.org)
  • Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies. (lsbio.com)
  • This antibody is specific for Homo sapiens keratin 19 (KRT19). (novusbio.com)
  • The furthest developed of these are bispecific tandem di-scFvs, known as bi-specific T-cell engagers (BiTE antibody constructs). (wikipedia.org)
  • The specificity of the binding is due to specific chemical constitution of each antibody. (wikipedia.org)
  • Collision-induced dissociation is used in mainstream applications to generate a set of fragments from a specific peptide ion. (wikipedia.org)
  • Different antibodies have the potential to discriminate among specific epitopes present on the antigen surface. (wikipedia.org)
  • Humanization can be necessary when the process of developing a specific antibody involves generation in a non-human immune system (such as that in mice). (wikipedia.org)
  • The DNA within the phage codes for the ZFPs expressed, so extracting and sequencing the DNA of bound phage provides information as to suitable amino acid configurations for binding a specific sequence. (wikipedia.org)
  • molecular
  • Alfred Nisonoff (1923-2001) was a 20th-century chemist who helped to experimentally determine the molecular structure of the antibody, and, as a result, made major contributions to the field of immunology. (wikipedia.org)
  • murine
  • Initially, murine antibodies were obtained by hybridoma technology, for which Jerne, Köhler and Milstein received a Nobel prize. (wikipedia.org)
  • 3. An isolated cell that produces the 6E6 HMGB1 monoclonal antibody (mAb) produced by the murine hybridoma deposited as ATCC Accession Number PTA-5433. (google.ca)
  • human
  • In cell culture studies, human monocytes were incubated with native LDL or oxidized LDL, in the presence of antibodies. (ahajournals.org)
  • Using a library of malondialdehyde (MDA)-modified polypeptides covering the complete amino acid sequence of human apoB-100, we have recently identified a large number of epitopes recognized by antibodies present in human plasma. (ahajournals.org)
  • and an H chain C region and L chain C region of a human antibody. (patentsencyclopedia.com)
  • Humanization is usually seen as distinct from the creation of a mouse-human antibody chimera. (wikipedia.org)
  • That is, after an antibody is developed to have the desired properties in a mouse (or other non-human), the DNA coding for that antibody can be isolated, cloned into a vector and sequenced. (wikipedia.org)
  • subsequent
  • The BCR is found only on the surface of B cells and facilitates the activation of these cells and their subsequent differentiation into either antibody factories called plasma cells or memory B cells that will survive in the body and remember that same antigen so the B cells can respond faster upon future exposure. (wikipedia.org)
  • 1995
  • The threonine and glutamic-acid proteases were not described until 1995 and 2004 respectively. (wikipedia.org)
  • Allele
  • Basically, the antibody paratope is polygenic, made up of three genes, V, D, and J. Each paratope locus is also polymorphic, such that during antibody production, one allele of V, one of D, and one of J is chosen. (wikipedia.org)
  • Glycosylation
  • Wright, Ann and Morrison, Sherie L., "Effect of Glycosylation on Antibody Function: Implications for Genetic Engineering," TIBTECH (1997) 15:26-32. (freepatentsonline.com)
  • The ability of an antibody to communicate with the other components of the immune system is mediated via its Fc region (located at the base of the "Y"), which contains a conserved glycosylation site involved in these interactions. (wikipedia.org)
  • different
  • Research and development is underway to create antibodies for diseases (such as rheumatoid arthritis, multiple sclerosis, Alzheimer's disease, Ebola and different types of cancers). (wikipedia.org)
  • Western Blot: Cytokeratin 19 Antibody (4E12) [NBP1-Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 19 monoclonal antibody. (novusbio.com)
  • The five different types of Fc regions allow antibodies to be grouped into five isotypes. (wikipedia.org)
  • Though the general structure of all antibodies is very similar, a small region at the tip of the protein is extremely variable, allowing millions of antibodies with slightly different tip structures, or antigen-binding sites, to exist. (wikipedia.org)
  • A large variety of different labels were introduced in the recent years, where 2-aminobenzamide (AB), anthranilic acid (AA), 2-aminopyridin (PA), 2-aminoacridone (AMAC) and 3-(acetylamino)-6-aminoacridine (AA-Ac) are just a few of them. (wikipedia.org)