Fractions membrane lipids. Medical search. Frequent questions

Lipid rafts or ordered lipid domains may play a role in the localization, transport, and function of different proteins. (hindawi.com)
Isolation and proteomic analysis of mouse sperm detergent-resistant membrane fractions: evidence for dissociation of lipid rafts during capacitation. (umassmed.edu)
In the present study, we examined potential contributory roles of membrane-associated, cholesterol-enriched lipid rafts/caveolae and their constituent proteins (e.g., caveolin-1 [Cav-1]) as potential sites for IL-1β−induced nitric oxide (NO) release in the isolated β-cell. (diabetesjournals.org)
Disruption of lipid rafts (e.g., with cyclodextrin) markedly reduced IL-1β−induced gene expression of inducible NO synthase (iNOS) and NO release from β-cells. (diabetesjournals.org)
IL-1β treatment also increased (within 20 min) the translocation of H-Ras into lipid rafts. (diabetesjournals.org)
Traditionally, lipid rafts have been defined by their insolubility in ice-cold Triton X-100 and low-buoyant density. (upenn.edu)
The ability of the B-subunit of cholera toxin to bind GM1 has been exploited to visualize membrane rafts by confocal microscopy in patching and capping experiments. (upenn.edu)
Biochemically, membrane rafts are isolated by solubolization in ice-cold Triton X-100 and separation of the low-buoyant density fractions from soluble material on sucrose density gradients. (upenn.edu)
We describe the isolation of Jurkat cell-specific membrane rafts using 2% Triton X-100. (upenn.edu)
Microdomains or lipid rafts greatly affect the distribution of proteins and peptides in the membrane and play a vital role in the formation and activation of receptor/protein complexes. (frontiersin.org)
A prominent example for the decisive impact of lipid rafts on signaling is LRP6, whose localization to the same lipid rafts domain as the kinase CK1γ is crucial for its successful phosphorylation and the subsequent activation of the signalosome, hence WNT/β-catenin signaling. (frontiersin.org)
Several studies suggested an involvement of lipid rafts in the WNT /β-catenin pathway. (frontiersin.org)
Even though LRP6 is homogenously distributed in the membrane and only a minor fraction is raft-associated, its localization to lipid rafts is vital for the activation of the LRP6 signalosome, hence wnt/β-catenin signaling. (frontiersin.org)
Lipid rafts are local assemblies of highly concentrated sphingolipids and cholesterol in the cell membrane. (frontiersin.org)
For most individual signaling pathway, this quantitative point of view would imply, that rafts clearly outnumber the membrane-associated pathway components, such as receptor and kinases. (frontiersin.org)
Therefore, a simulation model is needed, that takes a realistic number of lipid rafts as compartments into account. (frontiersin.org)
The plasma membranes of lens fibre cells are one of the most cholesterol rich membranes in the human body, forming lipid rafts and contributing to the biophysical properties of lens fibre plasma membrane. (aston.ac.uk)
The domains (lipid rafts) enriched in cholesterol and sphigolipid have been implicated to play important roles in a wide range of biological processes. (ncu.edu.tw)
The formation of lipid rafts and cavioli in biological membranes provides examples of cooperative association of lipids presumably assisted by the presence of cholesterol acting as a lineactant in the two-dimensional system of the membrane. (lu.se)

It is shown that cholesterol segregates preferentially around the receptors in both leaflets of the lipid bilayer, interacting robustly with specific transmembrane sites and creating a network of bridging microdomains. (iucr.org)
These differences can produce significant variations in membrane physical properties, in the location of phospholipids in a lipid bilayer and in their biological activity. (thermofisher.com)
The attachment position of the fluorophore determines whether it is located in the nonpolar interior or at the water/lipid interface when the phospholipid analog is incorporated into a lipid bilayer membrane ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
There is a resemblance to liposome to plasmalemma and the microscope picture served as first real evidence for cell membrane being a bilayer lipid structure. (ijpsr.com)
Liposome is an artificially prepared vesicle primarily composed of a lipid bilayer. (ijpsr.com)
Drug ionization reduces a drug's ability to cross a lipid bilayer. (pharmacology2000.com)
In the first part of this study outer membrane preparations of three Borrelia relapsing fever strains have been studied for pore-forming activity in the black lipid bilayer assay. (uni-wuerzburg.de)
In the second part, for the B. burgdorferi HB19 and p66 knock out strain HB19/K02, their outer membrane preparations were characterized in the black lipid bilayer assay. (uni-wuerzburg.de)
The purified mutant proteins were reconstituted into lipid bilayer membranes and the single-channel conductance was studied. (uni-wuerzburg.de)
A simplified model membrane system called a supported lipid bilayer (SLB) is often used to mimic the membrane of the APC. (lu.se)

Altered metabolism of membrane lipids has been proposed as a mechanism for the beneficial effects of postharvest calcium treatment on apple quality. (ashs.org)
The present study of lipids in "control" fruit was conducted as a prelude to further in-depth analyses of the effects of postharvest calcium and heat treatments on lipid metabolism in apples during and after cold storage. (ashs.org)
Fatty acids are liberated by the enzymatic action of phospholipase A on phospholipids ( Probes for Lipid Metabolism and Signaling-Section 17.4 ) and also by various other lipases. (thermofisher.com)
2004). LCPUFA are known to regulate hepatic lipid metabolism by modulating related enzyme expression at the level of gene transcription, mRNA processing and decay, and post-translational protein modifications, directing FA away from TAG storage and into oxidation and secretion (Clarke, 2004). (arasysperfector.com)
It includes bioactive compounds, such as cortisol and fatty acids, which may be linked via the mother's lipid metabolism. (biomedcentral.com)
Of these, especially cortisol plays a key role in gluconeogenesis, lipolysis and energy metabolism and thus potentially affects the lipid content and composition of human milk. (biomedcentral.com)
Bioactive sphingolipids in health and disease: lipidomic analysis, metabolism and roles in membrane signaling and autophagy. (sphingolipidclub.com)
This suggests that increasing brain acetyl-CoA metabolism may also influence lipid synthesis. (und.edu)
In addition, we performed experiments to determine whether treatment had an effect on disease progression, protein levels of enzymes involved in lipid metabolism, cytoskeletal structure, and myelin structure. (und.edu)
acetyl-CoA metabolism altered CNS lipid content in mice subject to EAE to suggest that treatment may alter CNS lipid metabolism in this model. (und.edu)
These data test the hypothesis that treatment may alter CNS lipid metabolism in mice subjected to EAE. (und.edu)
Therefore, targeting lipid metabolism and LD biogenesis may represent potential strategies for anti-ZIKV treatment development. (bvsalud.org)
We analyzed a donor Patatin-like phospholipase domain-containing protein 3 (PNPLA3) rs738409 polymorphism, hepatic expression of proteins involved in lipid metabolism by RT-PCR, hepatic stellate cell (HSC) activation by α-SMA immunohistochemistry and, one year after transplantation, histological progression of fibrosis due to Hepatitis C Virus (HCV) recurrence. (bvsalud.org)

With these molecules in hand, it became possible to investigate the phase behaviour of liquid ordered/liquid disordered supported membranes doped with the Gb 3 species by means of fluorescence and atomic force microscopy. (springer.com)
In contrast, globosides are neutral lipid molecules. (springer.com)
As reported earlier, the helical arrangement of the protein component of the vesicles facilitates image averaging and enables us to determine how cholesterol and phospho-lipid molecules are distributed in the surrounding matrix, using headgroup size as a means to discriminate between the two kinds of lipid. (iucr.org)
Here, we performed smFRET experiments on functionally active human A 2A adenosine receptor (A 2A AR) molecules embedded in freely diffusing lipid nanodiscs to study their intramolecular conformational dynamics. (nature.com)
Furthermore, even for height-matched adhesion molecules the 2D Kd of the TCR-pMHC interaction was found to be dependent on the relative density fraction of TCR to adhesion molecules, with low fractions of TCR molecules giving 2-3 times weaker binding. (lu.se)
The aim of the present study is to characterize the molecular effect of different classes of molecules on SC lipid and protein components. (lu.se)
Indeed, a number of signaling functions of (phospho)lipid molecules has been discovered. (cdc.gov)
While the locations and inferred H-bond connec- water molecules in a membrane protein, despite tivities of these internal water molecules are essen- being deeply buried and extensively H-bonded to tial, one would also like to quantify their mobility. (lu.se)

Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
During the last decades, the approach to overcome the complexity of biological membranes is to use simplified biomimetic models mainly consisting of binary or ternary mixtures of lipids [ 1 , 2 ]. (hindawi.com)
One of the most debated issues in the biophysics of lipids is lipid miscibility and its relevance to the function and organization of biological membranes. (hindawi.com)
In 1997, Simons and Ikonen renewed the interest in lipid phase studies, suggesting that lateral phase separation in biological membranes could be functionally important [ 3 ]. (hindawi.com)
Lipid biosensors are molecular tools used both in vivo and in vitro applications, capable of selectively detecting specific types of lipids in biological membranes. (qxmd.com)
Maintaining the barrier properties and the functioning (energy production, signal transduction, material transport etc.) of a biological membrane, a given membrane dynamics is required. (biomedcentral.com)
The biological functioning is assured by the dynamics of the membrane proteins, which may depend also on lipid-protein interactions. (biomedcentral.com)
Weak acid at acid pH: more lipid-soluble, becauses it is uncharged--the uncharged form more readily passes through biological membranes. (pharmacology2000.com)
Many evidences suggest that lipid domains coexist in biological membranes of living cells. (ncu.edu.tw)

Neutral lipid, glycolipid (GL), and phospholipid (PL) fractions were obtained by column chromatography followed by TLC separation of GL and PL classes. (ashs.org)
Thus, I aimed to mimic the phospholipid composition of plant ER membrane in yeast and evaluate the activity of the two plant CYPs expressed in this host and their interactions with a suitable reductase (POR). (europa.eu)
Lipids, as fatty acid content and composition of neutral (triacylglycerol-rich) and polar (phospholipid-rich) lipids, were measured with gas chromatography. (biomedcentral.com)
In phospholipid-rich lipids, high cortisol level was associated with higher myristic (14:0, mass %) and docosenoic (22:1 n − 9, mass %) acids. (biomedcentral.com)
High-resolution31P magic angle spinning (MAS) NMR spectroscopy is presented as a direct and non-perturbing method for measuring changes in surface charge density occurring in mixed phospholipid membranes upon binding of charged surface-active peptides. (ox.ac.uk)
Concurrent decrease in phosphatidylethanolamine content from 23% of total phospholipid to 7%, accumulation of 14C-PL to 2.6% of total phospholipid, and increase in lysophosphatidylethanolamine from 1 to 7% of total phospholipid durkng 2-hexanone + 14CHCl3 treatment indicated that the amine moiety of phosphatidylethanolamine polar head groups was the probable target of phosgene-lipid interaction, and that a degradative pathway existed which removed the abnormal phospholipid from hepatic membranes. (cdc.gov)

The ascorbate-dependent lipid peroxidation in the microsomal fraction of at term placentas and fetal membranes were studied. (karger.com)
Lipid peroxidation was significantly higher in the chorion and amnion compared to the placenta. (karger.com)
The results indicate that the ascorbate-dependent lipid peroxidation in the fetal membranes is specific for prostaglandin synthetase activity and that these tissues are a major site of prostaglandin synthesis. (karger.com)
Hemorrhoid therapy with medicinal plants: Astringency and inhibition of lipid peroxidation as Key factors. (scialert.net)
Ionising radiation (IR) is a cause of lipid peroxidation, and epidemiological data have revealed a correlation between exposure to IR and the development of eye lens cataracts. (aston.ac.uk)

Here we provide the first evidence to suggest that tyrosine phosphorylation of Cav-1 and subsequent interaction among members of the Ras signaling pathway within the membrane lipid microdomains represent early signaling mechanisms of IL-1β in β-cells. (diabetesjournals.org)
These low-density membrane microdomains have been referred to as detergent-resistant membranes, Triton-insoluble membranes, and Triton-insoluble floating fraction. (upenn.edu)

Furthermore, its protein-lipid composition, organization and size are subject to modification by physiological events, making it an important mediator of neuronal plasticity. (iucr.org)
The lipid composition of these membrane fractions changed far more with ripening of field grown than greenhouse grown fruit. (ashs.org)
Future studies will assess how differences in membrane lipid composition affect postharvest storage life of the fruit. (ashs.org)
A previous study of lipids from pericarp tissue of tomato fruit ranging from mature-green to red-ripe showed a large increase in total sterols accompanied by dramatic changes in sterol composition and conjugation with ripening. (ashs.org)
The research described here also help address basic scientific questions on the molecular regulation and biochemical understanding of the role of membrane composition on functionality of membrane-associated proteins of plant origin that may be involved in formation of yet questionable metabolic complexes or metabolons. (europa.eu)
This pathway is highly suited as a read out of the effect of membrane composition on membrane-bound complex performance in yeast. (europa.eu)
Capitalizing on the existing expertise in reconstruction of carnosic acid in yeast, I applied an interdisciplinary approach that involve metabolic engineering, lipid biochemistry and biophysics to study the role of membrane lipid composition on the CYPs activity and the assembly of the metabolic complex involved in this pathway. (europa.eu)
Influence of membrane lipid composition on cellular process i.e. (edu.au)
The role of membrane lipid composition in ageing i.e. (edu.au)
Summarizing the literature data, it seems that there is consensus that lipid composition and dynamics have the primary role in low-temperature adaptation and cold stresses. (biomedcentral.com)
This study revealed certain significant associations between milk cortisol and the fatty acid composition of human milk, indicating that cortisol might be one of the factors affecting the origin of the lipids in human milk. (biomedcentral.com)

Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
Localization of low-density detergent-resistant membrane proteins in intact and acrosome-reacted mouse sperm. (umassmed.edu)

Membrane probes include fluorescent analogs of natural lipids, as well as lipophilic organic dyes that have little structural resemblance to natural biomolecules ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
We offer fluorescent and, in a few cases, biotinylated analogs of five naturally occurring lipid classes-phospholipids, sphingolipids (including ceramides), fatty acids, triglycerides and steroids. (thermofisher.com)
Phospholipids are the principal building blocks of cell membranes. (thermofisher.com)
Certain fluorescent fatty acids ( Fatty Acid Analogs and Phospholipids-Section 13.2 ) are readily metabolized by live cells to phospholipids, mono-, di- and triacylglycerols, cholesteryl esters and other lipid derivatives. (thermofisher.com)
The membranes of Acanthamoeba palestinensis were studied by examination in fixed cells, and then by following the movements of glycerol- 3 H-labeled phospholipids by cell fractionation. (silverchair.com)
The changes in specific activity of glycerol- 3 H-labeled phospholipids in these membranes during incorporation, turnover, and after pulse-labeling indicated an ordered sequence of appearances of newly synthesized phospholipids, first in nuclei and RER, then successively in Golgi membranes, collapsed vesicles, and finally, plasma membrane. (silverchair.com)
These observations are consistent with the hypothesis that membrane phospholipids are synthesized, perhaps as integral parts of membranes, in RER and nuclei. (silverchair.com)
Subsequently, some of the newly synthesized phospholipids are transported to the Golgi complex to become integrated into the membranes of collapsed vesicles, which are precursors of the plasma membrane. (silverchair.com)
These are composed of natured phospholipids and may contain mixed lipid chains with surfactant properties (e.g. egg phosphatidyl ethanolamine). (ijpsr.com)
The Pomegranate culture is rich in selective cell membrane lipids (main ones: phospholipids, glycolipids, glycerides and free fatty acids). (vytrus.com)
A huge diversification of phospholipids, forming the aqueous interfaces of all biomembranes, cannot be accommodated within a simple concept of their role as membrane building blocks. (cdc.gov)
Radiolabel was not detected in neutral lipids of control or 2-hexanone-treated animals, but was enhanced 33-fold in phospholipids of 2-hexanone-treated animals. (cdc.gov)

It is concluded that weight loss improves the n-3 LCPUFA status of obese patients in association with significant amelioration in the biomarkers of oxidative stress, membrane FA insaturation, and n-3 LCPUFA biosynthesis capacity, thus representing a central therapeutic issue in the improvement of obesity-related metabolic alterations involved in the mechanism of hepatic steatosis. (arasysperfector.com)

In turn, only recently surface-sensitive techniques have explored the phase behaviour of lipid layers in contact with solid surfaces, such as supported lipid bilayers (SLBs) and supported layers of vesicles (SLVs). (hindawi.com)

Although fatty acids are ionized at neutral pH in water (pK a ~5), their pK a is typically ~7 in membranes, and thus a significant fraction of membrane-bound fatty acids are neutral species. (thermofisher.com)
Weak acid: in the protonated form, the molecule will be electrically neutral (no charge) and therefore be more lipid-soluble. (pharmacology2000.com)
31P MAS NMR was used to investigate mixed lipid membranes of neutral phosphatidylcholine and negatively charged phosphatidylglycerol where the molar fraction of the charged lipid was varied from 0 to 1. (ox.ac.uk)

Extrapolating/Translating these values to the membrane of a "normal sized" cell yields a raft abundance, that, by far, outnumbers the membrane-associated pathway components of most individual signaling pathway, such as receptor and kinases. (frontiersin.org)

Cyanobacterial and plant thylakoid membranes, mammalian mitochondria membranes, and for comparison, lysozyme were investigated. (biomedcentral.com)

Defects in the lysosomal breakdown of sphingolipids are the underlying cause of lipid storage disorders such as Niemann-Pick, Tay-Sachs, Krabbe and Gaucher diseases. (thermofisher.com)

Its unique signature that distinguishes it from other forms of cell death is the formation and accumulation of lipid hydroperoxides, particularly oxidized forms of polyunsaturated phosphatidylethanolamines (PEs), which drives cell death. (cdc.gov)
Fatty liver is excessive accumulation of lipid in hepatocytes. (msdmanuals.com)

In spite of the relevance of lipid-protein interactions, the phase behaviour of the lipid components of the cell membrane is believed to be of major importance to uncover some underlying principles behind the membrane function. (hindawi.com)
Cholesterol interactions with the receptor are apparently essential for stabilizing and maintaining its physiological architecture, since the transmembrane structure contracts, involving displacements of the helices at the outer membrane surface by ∼2 Å (1-3 Å), when this lipid is extracted. (iucr.org)
Concerning barrier properties, the dynamics of membrane lipids and lipid-protein interactions are thought to be more important. (biomedcentral.com)
At the high temperature end, however, it is not clear, whether proteins or lipids, or their specific interactions are the primary actors in maintaining membrane structure and/or stress signalling. (biomedcentral.com)
Organisms usually contain large numbers of sphingolipid subspecies (for a pathway based compilation, see www.sphingomap.org) and knowledge about the types and amounts is imperative because they influence membrane structure, interactions with the extracellular matrix and neighboring cells, vesicular traffic and the formation of specialized structures such as phagosomes and autophagosomes, as well as participate in intracellular and extracellular signaling. (sphingolipidclub.com)
Therefore, smFRET has been limited to studies of inter-receptor interactions in cellular membranes and receptors in detergent environments. (nature.com)

Effects of biophysical membrane properties on recognition of phosphatidylserine, or phosphatidylinositol 4-phosphate by lipid biosensors LactC2, or P4M. (qxmd.com)

and for investigating synaptosome recycling ( Probes for Following Receptor Binding and Phagocytosis-Section 16.1 ) and lipid-mediated signal transduction processes ( Probes for Signal Transduction-Chapter 17 ). (thermofisher.com)

The phase transitions of binary lipid mixtures are studied by a combination of Peltier-element-based adiabatic scanning calorimetry (pASC) and quartz crystal microbalance with dissipation monitoring (QCM-D). pASC, a novel type of calorimeter, provides valuable and unambiguous information on the heat capacity and the enthalpy, whereas QCM-D is proposed as a genuine way of determining phase diagrams by analysing the temperature dependence of the viscosity. (hindawi.com)

The results clearly highlight the influence of the different fatty acids of the Gb 3 sphingolipids on the phase behaviour and the binding properties of Shiga toxin B subunits, even though the membranes were only doped with 5 mol% of the receptor lipid. (springer.com)
Extraction procedures are optimized to precipitate and remove proteins and nucleic acids while maximizing solubilization of GSLs along with other lipids. (springer.com)
Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)
however, fatty acids transfer more readily between aqueous and lipid phases. (thermofisher.com)
Bioactive constituents in human milk include cortisol, fatty acids (FAs), such as polyunsaturated fatty acids (PUFAs), and parts of the milk fat globule membrane. (biomedcentral.com)
Among these signaling lipids, a particular group of oxygenated polyunsaturated fatty acids (PUFA), so called lipid mediators, has been thoroughly investigated over several decades. (cdc.gov)

2018 ). Here, we will solely focus on the less recognized globosides and in particular the globoside Gb 3 , which is the membrane receptor for vero toxins. (springer.com)
The cholinergic postsynaptic membrane is an acetyl-choline receptor-rich membrane mediating fast chemical communication at the nerve-muscle synapse. (iucr.org)
Structure of the ACh receptor in Torpedo postsynaptic membrane. (iucr.org)
Other, slightly less lipophilic probes are used as membrane markers of endocytosis and exocytosis ( Probes for Following Receptor Binding and Phagocytosis-Section 16.1 ). (thermofisher.com)
Thus, we investigated changes in P2X3 receptor distribution in the lipid raft membrane compartment, their phosphorylation state, as well as their function with patch clamping. (biomedcentral.com)
Anantin application caused preferential P2X3 receptor redistribution to the lipid raft compartment and decreased P2X3 serine phosphorylation, two phenomena that were not interdependent. (biomedcentral.com)
Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments. (biomedcentral.com)
Our simulation experiments indicate, that for receptor/raft ratios smaller than 1, i.e., when the number of raft compartments clearly exceeds the number of pathway specific membrane proteins, we observe significant decrease in LRP6 phosphorylation and downstream pathway activity. (frontiersin.org)

Liquid chromatography followed by mass spectrometry was used to analyse bovine eye lens lipid membrane fractions after exposure to 5 and 50 Gy and eye lenses taken from whole body 2 Gy irradiated mice. (aston.ac.uk)
Furthermore, one fraction obtained by anion exchange chromatography of the p66 knock out outer membrane protein preparation showed a uniform channel-forming activity with a single channel conductance of 300 pS. (uni-wuerzburg.de)

Sterol fraction, such as campesterol of this oil (a therapeutic component found in argan oil) has great potential against microbes, as well. (healthyy.net)
In this work, we study the properties of model membranes composed of unsaturated and saturated lipid, and the effect of sterol using fluorescence microscopy. (ncu.edu.tw)
Addition of sterol to unsaturated lipid/saturated lipid membranes results in a more well-defined bright/dark boundary. (ncu.edu.tw)
In these sterol-containing membranes, the bright and dark phases correspond to where the lipids are in the ld and lo phases, respectively. (ncu.edu.tw)
A slight structural difference in the sterol affects the membrane order significantly. (ncu.edu.tw)

However, despite their extensive use, there is a lack of systematic characterization of their binding properties in various membrane conditions. (qxmd.com)

This procedure yielded a consistent raft product that was enriched in cholesterol, gangliosides sphingo-myelin and membrane raft protein markers including lck and lat 1. (upenn.edu)
However, according to various experimental measurements, approximately 25 to 35 % of the cell plasma membrane is covered by nanoscopic raft domains with diameters ranging between 10 to 200 nm. (frontiersin.org)

Although cholesterol levels do not change significantly, IR dose-dependent formation of the oxysterols 7β-hydroxycholesterol, 7-ketocholesterol and 5, 6-epoxycholesterol in bovine lens nucleus membrane extracts was observed. (aston.ac.uk)
Replacing cholesterol with ergosterol substantially reduces the dark phase in unsaturated lipid/saturated lipid membranes. (ncu.edu.tw)

We mix the azo-PC with various fractions of either unsaturated or saturated lipids (DOPC or DPPC). (aps.org)
Comparing the azo-PC/DPPC membranes to the fully liquid azo-PC/DOPC membranes - both phase separated and single phase - we conclude that the finite yield-stress of the solid domains plays a key role in the buckling. (aps.org)
We found that the bright and dark phases coexist in model membranes containing unsaturated and saturated lipids such as DOPC/DPPC and POPC/DPPC. (ncu.edu.tw)

Plastids and microsomal membranes were isolated from pericarp tissue of mature green and red-ripe tell pepper fruit harvested from greenhouse and field grown plants. (ashs.org)
This study was conducted to determine whether similar changes occur in microsomal membranes derived from tomato fruit pericarp. (ashs.org)

Here, cryo-EM is used to examine the protein-lipid architecture of this membrane in tubular vesicles obtained from the (muscle-derived) electric organ of the Torpedo ray. (iucr.org)
Here, we analyse by cryo-EM the architecture of this membrane in vesicles isolated from the (muscle-derived) electric organ of the Torpedo ray, with view to defining the protein-lipid interplay required to achieve an optimal neurotransmitter response. (iucr.org)
Two previously undescribed structures were observed: collapsed cytoplasmic vesicles of cup shape, and plaques in food vacuole and plasma membrane similar in size to the collapsed vesicles. (silverchair.com)
It appeared that the plaques formed by insertion of collapsed vesicles into membranes and/or that collapsed vesicles formed by pinching off of plaques. (silverchair.com)
Collapsed vesicles from the plasma membrane by inserting into it as plaques. (silverchair.com)
When portions of the plasmalemma from food vacuoles, collapsed vesicles pinch off from their membranes and are recycled back to the cell surface. (silverchair.com)
We investigate ways to tune the morphology and permeability of giant unilamellar vesicles (GUVs) containing a photo-isomerizing lipid, azo-PC, which has an azobenzene moiety in one of its hydrocarbon tails. (aps.org)

Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)
Diconjugates appear to be formed at the cell membrane and may require the presence of the UDPGT tetramer. (medscape.com)
Monensin and nigericin are ionophores that carry protons across the plasma membrane, while the 2-deoxyglucose and NaN 3 deprive the cell from ATP and thus block energy-dependent pH maintenance. (elifesciences.org)
Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)
Due to their low toxicity and stable retention, some lipid probes are particularly useful for long-term cell tracing ( Tracers for Membrane Labeling-Section 14.4 ). (thermofisher.com)
The cell culture is also rich in its polyphenol fraction (plant chromophores), especially ellagic acid as a precursor of urolithins. (vytrus.com)
The two plant cell cultures have also been enriched with two components of plant origin (sugars) that play a key role as potent protectors and strengtheners of microbial cell membranes. (vytrus.com)
Each tight-junction strand within a plasma membrane associates laterally with another tight-junction strand in the apposed membrane of an adjacent cell to form a paired tight-junction strand, obliterating the intercellular space (kissing point). (pharmacology2000.com)
Lipid droplets (LD) are evolutionarily conserved lipid-enriched organelles with a diverse array of cell- and stimulus-regulated proteins. (bvsalud.org)
In many T cell activation studies, the SLB contains the nickel-chelating lipid DGS-NTA(Ni) to functionalize the SLB with histidine-tagged proteins. (lu.se)
It was found that increasing the concentration of DGS-NTA(Ni) both increased cell adhesion and the fraction of signaling cells. (lu.se)
Adding bovine serum albumin (BSA) functioned as a blocking agent, preventing unspecific cell adhesion and decreased the fraction of signaling cells down to a basal level. (lu.se)
Ferroptosis is an iron dependent form of cell death, that is triggered by the discoordination of iron, lipids, and thiols. (cdc.gov)
Inflammation may result from lipid peroxidative damage to cell membranes. (msdmanuals.com)

They randomly assigned 700 patients with ischemic cardiomyopathy (left ventricular ejection fraction [LVEF] ≤ 35%) and anatomy amenable to PCI and substantial myocardial viability to either PCI or optimal medical therapy. (medscape.com)

The results again demonstrate that the fatty acid of Gb 3 plays a pivotal role for the overall membrane organisation. (springer.com)
The postsynaptic membrane, by eliciting this response, plays a critical role in determining the efficacy and speed of synaptic transmission. (iucr.org)
These findings provide valuable insights into the role of membrane properties on the binding properties of lipid biosensors. (qxmd.com)
To understand the role of fats (lipids) in health, ageing and disease. (edu.au)
The balance between lipid- and protein-dynamics and the role of lipid-protein interaction in maintaining membrane functionality is still not fully understood. (biomedcentral.com)

C ) Outer membrane vesicle (OMV) quantification of WT and Δ mlaF deletion mutants harvested at 4 hr and quantified by measuring 3-deoxy-D- manno -oct-2-ulosonic acid (Kdo) levels using the Purpald assay. (elifesciences.org)
However, scattering originating from flagella was replaced by a term accounting for the oligosaccharide cores of the lipopolysaccharide leaflet of the outer membrane including its cross-term with the cellular body. (iucr.org)
Specifically, this approach provides detailed insight into structural features of the cellular envelope, including the distance of the inner and outer membranes, as well as the scattering length densities of all bacterial compartments. (iucr.org)
In the first part outer membrane preparation of three relapsing fever Borrelia were investigated. (uni-wuerzburg.de)
Histograms of conductance fluctuations were obtained from single-channel experiments with outer membrane preparations of B. hermsii, B. recurentis and B. duttonii. (uni-wuerzburg.de)
Furthermore the proteins of the outer membrane of B. duttonii were separated by chromatographic methods. (uni-wuerzburg.de)
In this study the expression of an outer membrane component of a putative drug efflux system of B. burgdorferi was shown for the first time. (uni-wuerzburg.de)

2.1.1 Assume that the soluble fraction of isolated circulating blood will be reconstituted into lipid fractions and the surface membrane of the respective column. (takepharmacologyexam.com)

For SLVs, experiments are so far restricted to pure lipids using dissipation-mode quartz crystal microbalance and heat-transfer studies [ 20 , 21 ]. (hindawi.com)

It is known that GSLs interact with both intracellular as well as exogenous proteins and are critical for membrane organisation, signalling, and recognition events. (springer.com)
The plasma membranes and intracellular membranes of live cells and the artificial membranes of liposomes represent a significant area of application for fluorescent probes. (thermofisher.com)

In GUVs with azo-PC and gel-forming DPPC, UV irradiation can induce large membrane deformations with notable buckling and pinning. (aps.org)

Through recent developments, Polarization Transfer Solid-State NMR (PT ssNMR) method together with almost complete peak assignment of SC components permits the detection of small changes in the molecular dynamics of the minor fluid lipid and protein components upon the added chemicals. (lu.se)

This work can be considered as a departure point for further investigations on more complex lipid mixtures displaying relevant phases such as the liquid-ordered phase and solid-lipid interfaces with biologically functional importance. (hindawi.com)
A comparison with previous issues reveal that the fraction of biologically oriented projects is increasing and we foresee that this trend will be sustained for some time. (lu.se)

In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
Total RNA was isolated from 200 mg rat adipose tissue using the RNeasy Lipid Tissue Midi Kit (with QIAzol). (qiagen.com)

After synthesis, lipids are flipped across the IM and anterograde transported to the OM. (elifesciences.org)
To test this hypothesis, we used Western blot analysis to measure protein levels of enzymes involved in lipid synthesis and lipid breakdown. (und.edu)
This is significant regarding the treatment of demyelinating diseases because developing a therapy to promote lipid synthesis and/or reduce lipid breakdown may prevent, possibly replace, the lipid loss found in EAE. (und.edu)

In contrast, neither membrane charge, nor membrane fluidity significantly affect the binding affinity of P4M to PI4P. (qxmd.com)

Recent findings reveal that lipid remodelling and increased LD biogenesis play important roles for Zika virus (ZIKV) replication and pathogenesis in neural cells. (bvsalud.org)

In nonalcoholic steatohepatitis animal models, an increased lipid droplet size in hepatocytes is associated with fibrogenesis. (bvsalud.org)

EAE resulted in a significant increase of cytosolic phospholipase A2 protein levels, but treatment returned those levels back to control to suggest treatment may modulate cytosolic phospholipase A2-mediated lipid breakdown. (und.edu)

Both techniques give consistent phase diagrams, which compare well with literature results, showing their capability to map the phase behaviour of pure lipids as well as lipid mixtures. (hindawi.com)
From a fundamental viewpoint, the interest in lipid phase behaviour stems from the nature and the thermodynamics of phase transitions, the mechanisms behind lipid miscibility and lateral phase separation, and the existence of critical phenomena [ 8 ]. (hindawi.com)
Information about the phase behaviour of supported lipid layers is relevant for lipid-based nanobiotechnology applications such as inmunosensors and lipid nanodevices [ 22 , 23 ]. (hindawi.com)

Correlation of lipid disorder with membrane protein dynamics has been studied with infrared spectroscopy, by combining data characterizing lipid phase, protein structure and, via hydrogen-deuterium (H/D) exchange, protein dynamics. (biomedcentral.com)
Around the low-temperature functioning limit of the biomembranes, lipids affected protein dynamics since changes in fatty acyl chain disorders and H/D exchange exhibited certain correlation. (biomedcentral.com)
Seemingly, at temperatures where protein self-dynamics allows large-scale H/D exchange, lipid-protein coupling is so weak that proteins prefer aggregating to limit the exposure of their hydrophobic surface regions to water. (biomedcentral.com)
In all membranes studied, dynamics seemed to be governed by lipids around the low-temperature limit, and by proteins around the high-temperature limit of membrane functionality. (biomedcentral.com)
This dynamics is adapted to the physiological conditions (e.g. temperature, light in photosynthetic organisms, different stresses) of the given membrane. (biomedcentral.com)

In membrane proteins, in contrast to lysozyme, the onsets of sizable H/D exchange rates were the onsets of irreversible denaturing as well. (biomedcentral.com)

The chemical shifts of the individual membrane lipids showed a simple variation in response to changes in the fraction of the negatively charged component phosphatidylglycerol. (ox.ac.uk)

The response depends on azo-PC fraction and UV light intensity. (aps.org)

In both fetal membranes, but not in the placenta, the reaction was enhanced by arachidonic acid and inhibited by indomethacin. (karger.com)
Total 14C binding in 2-HX-pretreated rats reached a plateau 2 hr post-14CHCl3 administration and was distributed 52% in protein, 41% in lipid, and 7% in acid soluble fractions 6 hr post-14CHCl3 administration. (cdc.gov)

Anatomy44

Organisms12

Diseases1

Chemicals and Drugs104

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes60

Disciplines and Occupations1

Technology, Industry, Agriculture7

Information Science3

Health Care3

Rafts19

Bilayer10

Metabolism13

Molecules8

Biological9

Phospholipid6

Peroxidation5

Microdomains2

Composition11

Localization2

Phospholipids12

Hepatic1

Supported lipid bilayers1

Neutral3

Signaling pathway1

Thylakoid membranes1

Cause of lipid1

Accumulation of lipid2

Interactions6

Biophysical1

Signal transduction1

Binary lipid1

Acids6

Receptor8

Chromatography2

Sterol5

Characterization1

Raft2

Cholesterol2

DOPC3

Microsomal2

Vesicles7

Cell15

Ventricular1

Role5

Outer7

Soluble fraction1

Experiments1

Intracellular2

DPPC1

Molecular1

Biologically2

Adipose tissue2

Synthesis3

Significantly1

Roles1

Hepatocytes1

Phospholipase1

Phase behaviour3

Dynamics5

Contrast1

Negatively charged1

Depends1

Acid2