• Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene. (wikipedia.org)
  • This asymmetry is specific to the C subfamily of ABC transporters and is generally not found in other transporters.ABCC1 is a highly conserved gene with polymorphisms occurring at very low frequencies of less than five percent. (wikipedia.org)
  • Polymorphisms in this gene are generally found in the form of a single-nucleotide polymorphism (SNP). (wikipedia.org)
  • The ABCC1 gene, the gene that encodes the ABCC1 protein, is found on chromosome 16 within the nucleus. (wikipedia.org)
  • A G2168A polymorphism and polymorphisms found in the 3'-UTR region of the gene have been shown to have a connection with increased susceptibility to lung cancer, especially in Chinese populations. (wikipedia.org)
  • When researchers compare their modified mice to control Black 6 mice, they may observe phenotypic differences that result from passenger mutations in 129 mouse DNA, rather than from changes to their actual gene of interest, according to the Ghent team. (the-scientist.com)
  • However, in 2011, researchers at Genentech showed that many Casp1 knockout mice also harbored a mutated Casp11 gene from 129 strain mice. (the-scientist.com)
  • Ting and colleagues in recent years have published studies showing that mice lacking the NLRP12 gene are highly susceptible to excessive inflammation, including experimental colon inflammation and associated colon cancer. (integrativepractitioner.com)
  • Ting and colleagues in this study therefore sought to determine whether mice lacking the NLRP12 gene are more susceptible to obesity. (integrativepractitioner.com)
  • The scientists fed mice that lacked the NLRP12 gene and ordinary mice a high-fat diet for several months. (integrativepractitioner.com)
  • They developed a 'knockout' mouse, in which the gene that codes for Abcc10 was missing, or knocked out. (news-medical.net)
  • The researchers isolated cells from the knockout mice and tested the cells' reactions to taxanes and two other anticancer drugs, vincristine and Ara-C. Compared to cells from normal mice that still possessed the gene for Abcc10, the knockout mouse cells were much more sensitive to the drugs. (news-medical.net)
  • And to investigate the potential role of p66Shc, we generated streptozotocin (STZ)-induced diabetic mice carrying p66Shc knockout (−/−) gene. (nature.com)
  • We further investigated the role of the RRBP1 gene using a transgenic mouse model and a human cell model. (biomedcentral.com)
  • Their experiments in lab mice and in human blood and tissue samples further showed that this gene not only is associated with the disease, as predicted computationally, but is also likely to play a major causal role. (stanford.edu)
  • When this clock gene is mutated, mice have impaired circadian behaviors and develop obesity. (knowingneurons.com)
  • FUNCTION: [Summary is not available for the mouse gene. (utsouthwestern.edu)
  • Studies in mice and human suggest that this gene maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression. (utsouthwestern.edu)
  • Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (utsouthwestern.edu)
  • Mutations in the PIK3R1 gene encoding phosphatidylinositol 3-kinase (PI3-kinase) have been found to cause severe insulin resistance [Baynes et al. (lu.se)
  • Germline mutation of one TP53 allele is found in patients with Li-Fraumeni syndrome who generally inherit a mutated TP53 gene from an affected parent. (medscape.com)
  • The mice had increased oral glucose tolerance and an increased insulin sensitivity compared to normal mice. (bruker.com)
  • Mice with a mosaic (mixed) muscle-specific knockout pattern also showed the same qualities of increased glucose tolerance and resistance to obesity and diabetes. (bruker.com)
  • Experiments repeated with liver-specific AIF knockout mice showed the same result--increased glucose tolerance and insulin sensitivity. (bruker.com)
  • A multisystem AIF knockout mouse, the Harlequin mouse, was also found to have the same qualities of improved insulin sensitivity, glucose tolerance, and resistance to diet-induced obesity and diabetes. (bruker.com)
  • The experiment returned the AIF knockout mice to normal glucose tolerance and plasma peak insulin levels. (bruker.com)
  • Because the AIF knockout shifted the metabolite balance--decreased ATP, decreased NAD, and increased AMP--activation of AMPKinase might have stimulated catabolic processes including glucose uptake and fatty acid oxidation. (bruker.com)
  • The knockout mice also showed signs of insulin resistance, which involves a reduced ability to clear glucose from the bloodstream and tends to follow the development of obesity. (integrativepractitioner.com)
  • Type 2 diabetes mellitus (T2DM) is a progressive metabolic disease characterized by pancreatic β-cell dysfunction and peripheral insulin resistance, leading to defects in glucose metabolism and chronic low-grade inflammation. (frontiersin.org)
  • Comparing to SUC-fed mice, HFCS-fed mice showed lower body weight but higher circulating glucose and insulin levels. (tamu.edu)
  • It seemed that the mice were always in an inactive, fasting phase, so more glucose was converted into fat for storage instead of being burned for energy. (knowingneurons.com)
  • MARCH1 KO mice of both sexes were more glucose tolerant than WT mice. (arizona.edu)
  • Senp2 knockout mice exhibited a lipodystrophy-like phenotype. (deepdyve.com)
  • 2010). Quickly, the man mouse phenotype contains insulin level of resistance with hyperinsulinemia, hyperlipidemia and hyperglycemia, while feminine mice possess isolated insulin hyperinsulinemia and level of resistance, but are normolipidemic and normoglycemic. (findadig.com)
  • Rag/MKR mice possess an identical metabolic phenotype weighed against MKR mice and also have been previously defined (Zelenko et al. (findadig.com)
  • Homozygous CyP-D knock-out mice display regular phenotype (Basso et al. (mingsheng88.org)
  • 19-25 TMPRSS2 knock-out mice have no phenotype in the absence of infection, 26 indicating that inhibiting TMPRSS2 function might not be associated with substantial unwanted side effects. (rsc.org)
  • Although less fat stor- adqcKO pose lipid storage in adipocyte-speci﫿c Senp2 knockout mice fed age was shown in Senp2 adipose tissues, the increased with high-fat diets (HFD). (deepdyve.com)
  • In mice, we showed that NLRP12 reduces inflammation in the gut and in adipose fat tissues. (integrativepractitioner.com)
  • Xenografts and liver organ tissues from OP449-treated mice demonstrated a 64% and 70% decrease in STAT5 activation, weighed against handles (p 0.01 and p=0.06, respectively). (findadig.com)
  • Type-2 diabetes, on the other hand, results from a phenomenon called insulin resistance: the tendency of cells in tissues throughout the body - but especially in fat, liver and muscle - to lose sensitivity and ignore the insulin's "gravy train" signal. (stanford.edu)
  • Interestingly, we also found that ghrelin deletion exacerbates HFCS-induced adiposity and inflammation in adipose tissues, as well as whole-body insulin resistance. (tamu.edu)
  • The levels of insulin receptor were generally higher in insulin-responsive tissues (especially the liver) from female MARCH1 KO mice compared to males, with the potential to account in part for the differences between male and female MARCH1 KO mice. (arizona.edu)
  • However, reduced production of TNF- α was detected in bone-marrow-derived macrophages (BMDM) from NOD1, NOD2, and Rip2 KO mice compared to C57BL/6. (hindawi.com)
  • The wild-type strain C57BL/6 mice were purchased from the Federal University of Minas Gerais (UFMG, Belo Horizonte, Brazil). (hindawi.com)
  • The initial finding that Abcc10, a member of a ubiquitous family of proteins called ATP-binding cassette transporters, thwarts taxanes' anti-tumor activity was something of a surprise, says Hopper-Borge, because none of the other family members seem to have that ability. (news-medical.net)
  • The results provide the first evidence from living organisms that Abcc10 is a cell's built-in protection against the effects of powerful drugs, and raises the possibility of using Abcc10 inhibitors to break down that resistance and sensitize tumor cells to anticancer agents. (news-medical.net)
  • Methods Using our conditional TrIP knockout mouse model, we have performed syngeneic tumor challenges in CD8 + T cell-specific TrIP knockout mice (TrIP fl/fl E8i cre ). (bmj.com)
  • In addition to increased tumor resistance, we have also found that tumors harvested from our TrIP fl/fl E8i cre knockout mice contain twice as many infiltrating T cells compared to their WT counterparts. (bmj.com)
  • Our CD8 + T cell-specific TrIP knockout mice are resistant to tumor challenge and show more robust tumor CD8 + T cell infiltrate. (bmj.com)
  • Though chemotherapy drugs show initial positive response, tumor acquires resistance gradually and patients experience continued tumor growth and metastatic disease. (springernature.com)
  • To provide a better understanding of β-catenin's function in breast tumor progression and metastasis formation, we have performed functional in vivo analysis in the MMTV-PyMT mouse model. (unibas.ch)
  • From the different mouse tumor genotypes, cell lines have been established to further characterize and examine the effects of an altered canonical Wnt signaling output. (unibas.ch)
  • These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)
  • Right here we present results helping an anti-neoplastic aftereffect of OP449 on individual MDA-MB-231 breast cancers xenografts and murine MVT-1 tumors in the Rag /MKR immunodeficient mouse style of hyperinsulinemia (Zelenko, et al. (findadig.com)
  • Results Our data show that CD8 + T cell-specific TrIP knockout mice (TrIP fl/fl E8i cre ) are resistant to growth of syngeneic tumors. (bmj.com)
  • [ 2 ] Targeted disruption of TP53 in the mouse leads to the development of various tumors (see image below). (medscape.com)
  • Tumors that developed in mice deficient in INK4A were enhanced by the topical application of carcinogens and ultraviolet light. (medscape.com)
  • We are currently generating prostate-specific Usp26 knockout mouse models using CRISP/Cas9 techniques to elucidate the role of USP26 in PCa. (tulane.edu)
  • Unexpectedly, 8-oxoguanine DNA glycosylase1 (Ogg1) knockout mice show enhanced stimuli-driven IFN expression that confers increased resistance to viral and bacterial infections and allergen challenges. (lu.se)
  • In a mouse model of bronchiolitis induced by RSV infection, functional ablation of OGG1 by a small molecule inhibitor (TH5487) enhances IFN-λ production, decreases immunopathology, neutrophilia, and confers antiviral protection. (lu.se)
  • In earlier work, Elizabeth A. Hopper-Borge, Ph.D., an assistant professor at Fox Chase, showed that Abcc10 confers resistance to a number of anticancer agents, particularly taxanes, which include paclitaxel (Taxol) and docetaxel (Taxotere). (news-medical.net)
  • Our findings suggest that HFCS and SUC have differential effects on lipid metabolism: while sucrose promotes obesogenesis, HFCS primarily enhances inflammation and insulin resistance, and ghrelin confers protective effects for these metabolic dysfunctions. (tamu.edu)
  • Scientists from the University of North Carolina School of Medicine discovered that the anti-inflammatory protein NLRP12 normally helps protect mice against obesity and insulin resistance when they are fed a high-fat diet. (integrativepractitioner.com)
  • The study , published in Cell Host & Microbe , showed that NLRP12's anti-inflammatory effect promotes the growth of a "good" family of gut-dwelling bacteria, called Lachnospiraceae , that produce small molecules butyrate and propionate, which in turn promote gut health and protect mice against obesity and insulin resistance. (integrativepractitioner.com)
  • Researchers can genetically modify Black 6 mice using Black 6 ESCs, or they can use CRISPR/Cas to make knockout mice in just about any background. (the-scientist.com)
  • To understand the role of potential kinase signaling in 5FU resistance in OSCC, a CRISPR based kinome-wide high throughput screening was performed using a lentiviral sgRNA library knocking out 840 kinases individually with a total number of 3214 sgRNA constructs, followed by treatment with a sub lethal dose of 5FU for 48h after which cell death was measured in high content analyzer using a fluorescent cell viability dye. (springernature.com)
  • The protein resides intracellularly on the basolateral side of the plasma membrane which differs from other ATP-binding cassette transporters that are found on the apical side of the membrane. (wikipedia.org)
  • We further found that SET domain bifurcated 1 (Setdb1) was a SUMOylated protein and that SUMOylation promoted Setdb1 occupancy on the promoter locus of Pparg and Cebpa genes to suppress their expressions by H3K9me3. (deepdyve.com)
  • We found that SET domain bifurcated 1 Since no difference in food intake was observed between adqcKO f/f (Setdb1) was a SUMOylated protein and that Senp2 de-SUMOylated Senp2 and Senp2 mice fedeitherwithNCD or HFD and regulated Setdb1 action in trimethylation at histone 3 lysine 9 (Supplementary Figure S2A). (deepdyve.com)
  • The Ghent researchers looked through the sequences for differences between Black 6 mice and the 129 strains that would affect protein production. (the-scientist.com)
  • New findings by Fox Chase Cancer Center researchers identify one protein, Abcc10 (also known as Mrp7), as being intimately involved in resistance to certain drugs used to treat breast, ovarian, lung, and other cancers. (news-medical.net)
  • It had been suggested that other proteins might take over for Abcc10 if that protein were knocked out, but the researchers found no evidence suggesting that had happened. (news-medical.net)
  • The fact that mice lacking the protein have no obvious health problems is encouraging, suggesting that Abcc10 inhibitors could be used in human patients without causing side effects that might be expected to result from interfering with the pump's normal functions. (news-medical.net)
  • Our results so far suggest that this protein may be a clinically relevant target, but we need to do more studies to find out for sure. (news-medical.net)
  • Western blot analysis on extracts of both embryonic fibroblasts and liver nuclear membranes from homozygous mice confirmed that no detectable protein was expressed from this allele. (jax.org)
  • The latter represents a conformationally modified form of a normal cellular PrP C , which is a normal host protein found on the surface of many cells, in particular neurons. (medscape.com)
  • The role of the smoothelin-like 1 (SMTNL1) protein in mediating vascular smooth muscle contractile responses to intraluminal pressure was examined in resistance vessels. (duke.edu)
  • however, augmented protein kinase C (PKC) signaling was found to contribute to the increased myogenic reactivity of SMTNL1 KO vessels across the 60-120 mmHg pressure range. (duke.edu)
  • In this study, we found that RING finger protein 4 (RNF4), a RING finger E3 ubiquitin ligase, is required for the RIPK1 autophosphorylation and subsequent cell death. (mdpi.com)
  • Somatic mutations at the phosphotyrosine-binding pocket of the C-terminal SH2 domain of GTPase-activating protein RASA1 have been found in a subset of Basal-cell carcinoma (BCC) [Friedman, 1995]. (lu.se)
  • 2003). Selective Cellular uptake of fatty acids and following storage in the form of disruption of Pparγ2 or adipocyte-speci﫿c Pparγ knockout leads TGs in adipocytes are key steps in lipid storage. (deepdyve.com)
  • We first analyzed breast cancer progression and metastasis formation in the complete absence of β-catenin by conditional knockout. (unibas.ch)
  • Type 2 diabetes is typified by insulin-resistance in adipose tissue, skeletal muscle, and liver, leading to chronic hyperglycemia. (arizona.edu)
  • Since 129 strain mice are difficult to breed, researchers typically transfer their ESCs into the embryos of Black 6 mice and then repeatedly backcross the animals with Black 6 mates while selecting for their 129 strain-derived transgene, slowly eliminating 129 strain DNA surrounding the transgene through recombination. (the-scientist.com)
  • The researchers showed that the passenger mutation to Casp11 was partly responsible for the animals' resistance to shock. (the-scientist.com)
  • In recent years, researchers have found evidence that inflammation in the gut and in where fat is deposited promotes obesity. (integrativepractitioner.com)
  • The absence of NLRP12 in these mice led to increased signs of inflammation in the gut and in fat deposits, but it wasn't clear how this led to extra weight gain until the researchers moved the animals from one facility to another. (integrativepractitioner.com)
  • Following standard safety protocols to prevent disease spread, the researchers dosed the mice with antibiotics before the move. (integrativepractitioner.com)
  • Medical researchers would like to find ways of counteracting such resistance, but first they must understand why and how it happens. (news-medical.net)
  • For example, researchers found that 43 bacterial taxa were significantly different between Chinese obese individuals with T2DM and healthy people by LEfSe analysis, and Acidaminococcales , Bacteroides plebeius and Phascolarctobacterium sp. (frontiersin.org)
  • The researchers found that Bmal1 knockout mice were always in an insulin-resistant mode, similar to their inactive, fasting phase. (knowingneurons.com)
  • Newswise - Hamilton, ON (May 14, 2020) - McMaster University researchers have discovered a combination punch to treat drug-resistant infections that is showing promise based on testing in mice. (newswise.com)
  • Researchers found that a natural product called dephostatin is an effective partner for the antibiotic colistin in treating infections caused by the bacteria Salmonella . (newswise.com)
  • In their study, researchers found that dephostatin does not kill Salmonella or stop it from growing. (newswise.com)
  • While the initial findings were done using a method of experimentation called high-throughput screening, the researchers were excited to find that co-administering dephostatin and colistin in mice with a lethal Salmonella infection significantly prolonged animal survival and used a lower concentration of colistin than is normally required for treatment, thereby reducing its toxic effect. (newswise.com)
  • To investigate the function of CD97 in colorectal carcinogenesis, transgenic Tg(villin-CD97) mice overexpressing CD97 in enterocytes were generated and subjected to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated tumorigenesis. (nih.gov)
  • FLICKR, STEFANO Passenger mutations-unwanted alterations that accompany intentional genetic modifications-can confound experiments using certain types of transgenic mice. (the-scientist.com)
  • The new tool "empowers the scientific community to look a little closer" at the transgenic mice they are using, said Soren Warming , a senior scientist at Genentech who was not involved in the study. (the-scientist.com)
  • Passenger mutations affect experiments using transgenic animals models called congenic mice, whose modified genetic material comes from a different genetic background than the rest of their genome. (the-scientist.com)
  • Knockout and other transgenic mice are traditionally made by genetically modifying embryonic stem cells (ESCs) in a dish and then inserting them into a recipient mouse embryo. (the-scientist.com)
  • There are now ways to make transgenic mice that are less risky. (the-scientist.com)
  • But a substantial quantity of biology research has been built on traditionally constructed transgenic mice, and many labs continue to use them. (the-scientist.com)
  • Using TCR-transgenic TrIP KO mice, we have stimulated with varying affinity peptides to investigate how TrIP may alter activation/kinetics. (bmj.com)
  • Initial metabolic analysis of the mice who had OxPhos deficient skeletal muscle at 8 weeks revealed a surprise. (bruker.com)
  • People don't realize that recombination occurs very infrequently and that you're going to end up with this chunk of DNA from one strain on the background of the other," said Jake Lusis , who studies mouse genetics and cardiovascular and metabolic disorders at the University of California, Los Angeles, and was not involved in the study. (the-scientist.com)
  • Growth hormone (GH) plays important roles in maintaining metabolic equilibrium via GH receptor (GHR), illustrated previously by our GHR tissue specific knockout mouse models in either the liver (LKO) or adipose tissue (AKO). (tus.ac.jp)
  • PCOS is a heterogeneous disease characterized by hyperandrogenism, dysfunctional ovulation, and polycystic ovary morphology, accompanied by metabolic abnormalities, such as insulin resistance (IR) and obesity. (biomedcentral.com)
  • 2007) investigate whether defects in mitochondrial respiration cause T2DM and obesity using AIF to generate models of OxPhos deficiency in mice. (bruker.com)
  • RRBP1 deficiency in mice caused hyporeninemic hypoaldosteronism, resulting in lower blood pressure, severe hyperkalemia, and sudden cardiac death. (biomedcentral.com)
  • They engineered AIF knockout mice with muscle- or liver-specific OxPhos deficiency and confirmed that the animals were a valid model for OxPhos dependency of insulin resistance. (bruker.com)
  • In both KO and wildetype animals, equal amounts of C. jejuni can be found in the intestine, but only in the KO mice the bacterium is also found in the liver, suggesting that lower levels of available sulfate and sulfomucins enables the transit of the bacteria through the gut epithelial. (thermofisher.com)
  • To understand the reciprocal inter-organ regulatory routes, we reported the construction of liver/adipose double GHR knockout mouse model (DKO). (tus.ac.jp)
  • In an animal model of PFIC3, Abcb4 (Mdr2) knockout mice cannot excrete phospholipid into bile and develop progressive liver disease characterized by portal inflammation, proliferation of bile ducts, and fibrosis. (medscape.com)
  • adqcKO Mechanistically, adipocyte Senp2 de﫿ciency caused the downregula- Senp2 mice exhibit an ectopic lipid accumulation and tion of Pparg and Cebpa as well as their downstream target genes insulin resistance related to lipid storage. (deepdyve.com)
  • KO) female mice exhibit excessive weight gain and excessive visceral adiposity when reared on standard chow diet, without increased inflammatory cell infiltration of adipose tissue. (arizona.edu)
  • Some genetically engineered mice harbor unwanted mutations that hitchhike alongside desired modifications, affecting experimental outcomes. (the-scientist.com)
  • They found that 1,084 genes in 129 strain mice have insertions, deletions, or single-nucleotide mutations that cause them to diverge in sequence from Black 6 mice. (the-scientist.com)
  • 2005) have shown that deletion of mitochondrial flavoprotein apoptosis inducing factor (AIF) in mice leads to progressive OxPhos dysfunction. (bruker.com)
  • SMTNL1 deletion was associated with enhanced myogenic tone in vessels isolated from male, but not female, mice. (duke.edu)
  • Based on these findings, we conclude that deletion of SMTNL1 contributes to enhancement of pressure-induced contractility of mesenteric resistance vessels by influencing the activity of myosin phosphatase. (duke.edu)
  • We found that functional inhibition, genetic ablation, and inactivation by post-translational modification of OGG1 significantly augment IFN-λ expression in epithelial cells infected by human respiratory syncytial virus (RSV). (lu.se)
  • Our method finds groups of GO terms significantly over-represented amongst differentially expressed genes which are not found by Fisher's tests on individual GO terms. (biomedcentral.com)
  • The NLRP12-knockout mice ate and drank no more than their healthy cousins but accumulated significantly more fat and became heavier. (integrativepractitioner.com)
  • Moreover, p66Shc (−/−) diabetic mice show significantly less cognitive dysfunction and decreased levels of OS and the numbers of microglia. (nature.com)
  • The survival of Rrbp1 -KO mice significantly decreased under high potassium intake due to lethal hyperkalemia-induced arrhythmia and persistent hypoaldosteronism, which could be rescued by fludrocortisone. (biomedcentral.com)
  • To confirm the finding from the kinome screening, cell viability and cell death assays were performed which suggest that knocking out MINK1 (MINK1 KO) significantly resenitized chemoresistant cells to 5FU (Fig. 2A&B). (springernature.com)
  • To understand the mechanism by which MINK1 regulates 5FU resistance, we performed a high-throughput phosphorylation profiling in MINK1 WT and MINK1 KO 5FU resistant cells (Fig. 3A&B). From this study, we found p- p53 (Ser33) and p-53(Ser15) to be significantly up-regulated in MINK1KO cells whereas p-AKT (Ser473) and p-MDM2 (Ser166) were found to be down-regulated in MINK1KO cells as compared to MINK1WT cells. (springernature.com)
  • When exposing the C3-knockout INS-1 cells to β cells autophagy inducers (palmitate and IAPP), we observed significantly increased cell death caused by autophagy insufficiency. (lu.se)
  • We found that IRIS-1 expressing cells displayed significantly higher expression levels of Urocortin 3 and Pdx 1 (markers of mature β cells, which loss marks the beginning of β cells dedifferentiation), suggesting that IRIS-1 may be required for maintaining β cells identity and function. (lu.se)
  • Recent studies show that certain phenotypes of gliomas such as malignancy, resistance to therapy, and relapses are associated with the epigenetic alterations of tumour-specific genes. (hindawi.com)
  • In adipocyte Senp2-de﫿ciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)
  • Interferons (IFNs) are secreted cytokines with the ability to activate expression of IFN stimulated genes that increase resistance of cells to virus infections. (lu.se)
  • The most common way to do this is to perform Fisher's exact tests to find GO terms which are over-represented amongst the genes declared to be differentially expressed in the analysis of the microarray experiment. (biomedcentral.com)
  • Since all genes annotated to a given GO node are also annotated to all its parents, closely related nodes may be found separately significant. (biomedcentral.com)
  • We identified interactions between genes involved in mitochondrial dysfunction ( POLG2 ), cholesterol metabolism ( SOAT2 ), lipid metabolism ( CYP11B2 ), cell adhesion ( EZR ), cell proliferation ( MAP2K5), and insulin resistance ( IGF1R ). (biomedcentral.com)
  • Using Nod-deficient mice, several in vivo studies have demonstrated a role for Nod1 and Nod2 in host defense against pathogens such as Helicobacter pylori, Listeria monocytogenes, Staphylococcus aureus, and Legionella pneumophila [ 7 - 10 ]. (hindawi.com)
  • NOD1, NOD2, and RIP2 genetically deficient mice (NOD1 −/− , NOD2 −/− , and RIP2 −/− ) were kindly gifted by Dr. Richard Flavell (Yale University) and maintained in the animal facility of the University of São Paulo (FMRP/USP). (hindawi.com)
  • Wild-type and deficient mice were maintained at UFMG and used at 6-8 weeks of age. (hindawi.com)
  • We found that the lipid accumulation in the adipose tissue of DKO was further aggravated compared to AKO, while the insulin resistance and hepatic steatosis were abolished in DKO, compared to LKO. (tus.ac.jp)
  • This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. (wikipedia.org)
  • For example, sulfate transporter knockout (KO) mice show low levels of circulating serum sulfate and reduction of sulfomucins in the gut. (thermofisher.com)
  • It is also found in the endothelium cells of the blood-brain barrier. (wikipedia.org)
  • As these proteins accumulate, they begin to form plaques that interfere with signaling between cells of the nervous system found within the brain. (wikipedia.org)
  • Mice primed with either ovalbumin or methylated BSA also showed enhanced antigen-specific immune response of both T and B cells, an immunological abnormality that exacerbated antigen-induced arthritis. (jci.org)
  • Until recently, these cells almost always came from 129 strain mice, a group of substrains of mice that produce ESCs that are unusually robust and easy to manipulate. (the-scientist.com)
  • In the new research, Hopper-Borge and colleagues wanted to further explore, in both cultured cells and mice, the role of Abcc10. (news-medical.net)
  • For shot of individual breast cancers cells, an immunodeficient MKR+/+ mouse was used. (findadig.com)
  • Specifically, ICC was found to be the primary source of cancer-associated fibroblasts, while HCC exhibited disrupted metabolism and greater individual heterogeneity of T cells. (bvsalud.org)
  • An immunohistochemical study revealed renin accumulation in the juxtaglomerular cells of Rrbp1 -KO mice. (biomedcentral.com)
  • Reprogramming resistant cells to undergo drug induced cell death is a feasible approach to overcome drug resistance. (springernature.com)
  • As compared to the β-catenin knockout cells, these cells were viable, suggesting a key role of β-catenin's adhesion function in cell survival. (unibas.ch)
  • In that regard, a disruption in the balance of pro-inflammatory CD4+ T helper (Th) 17 cells and homeostatic regulatory T (Treg) cell responses in favor of Th17 cells has been reported in non-cancer-related aging humans and mice. (tulane.edu)
  • Type 2 diabetes (T2D) is characterised by defective insulin exocytosis from the pancreatic β cells, accompanied by insulin resistance. (lu.se)
  • We found that C3 is upregulated in pancreatic islets during T2D as a factor against β cells dysfunction caused by attenuated autophagy. (lu.se)
  • C3 was found intracellularly in isolated human pancreatic β cells. (lu.se)
  • We found that IRIS-1 localizes in the nuclei of pancreatic β cells. (lu.se)
  • mouse, which lacks older B and T lymphocytes. (findadig.com)
  • Insuf﫿cient adipose lipid storage is asso- ciated with many pathological conditions including hyperlipidemia, insulin resistance, and type 2 diabetes. (deepdyve.com)
  • Adipocyte Senp2 de﫿ciency resulted in less adipose lipid storage accompanied by an ectopic fat accumulation and insulin resistance under high-fat diet feed- ing. (deepdyve.com)
  • As expected, knockout mice treated with paclitaxel had smaller spleens and thymus glands and underdeveloped bone marrow, compared to normal mice treated with the same drug. (news-medical.net)
  • The second project presented in this thesis aimed to examine mechanisms of evasive resistance to sorafenib in hepatocellular carcinoma. (unibas.ch)
  • Dephostatin appears to knock out two important regulatory pathways that control Salmonella virulence and antibiotic resistance mechanisms," said Coombes, corresponding author and a professor in the Department of Biochemistry and Biomedical Sciences at McMaster University. (newswise.com)
  • 2019). We are currently working on generating testis-specific Usp26 knockout mice to elucidate the mechanisms of USP26 in spermatogenesis and sperm maturation. (tulane.edu)
  • We used quantitative Western blotting to observe Lcn2 serum levels in lean and obese mouse models. (diabetesjournals.org)
  • This, in turn, impaired insulin sensitivity in mouse models. (arizona.edu)
  • 2003]. The absence of STAT5B is associated with diminished post-natal growth, as demonstrated by mouse knockout models [Udy et al. (lu.se)
  • I'd like to stress that we did this work in a mouse model,' Hopper-Borge says. (news-medical.net)
  • The hyperinsulinemic MKR mouse model continues to be previously defined (Ferguson, et al. (findadig.com)
  • 2009). We have generated an Usp26 KO mouse model, the first of its kind, and found that disruption of Usp26 causes male subfertility associated with spermatogenesis defects in mice (Biol Reprod. (tulane.edu)
  • Cannabinoids may impact the balance between the inflammatory response and also host resistance with dysregulation of cytokine manufacturing. (kingsilvernews.com)
  • Shandong First Medical University (SDFMU) (Shandong Academy of Medical Sciences), founded in February 2019, is a key construction university in Shandong Province and the largest medical science research institution in Shandong Province. (tus.ac.jp)
  • We found that the mutant mice were resistant to OS and produced less ROS in the brain. (nature.com)
  • Direct evidence linking the INK4A locus to tumorigenesis was provided by the targeted disruption of exon 2 of INK4A in mice. (medscape.com)
  • The overactivation of the sympathetic system and the promotion of a pro-inflammatory state in the adipose tissue [ 8 ] are common features of insulin resistance [ 9 - 12 ]. (biomedcentral.com)
  • These processes to severe lipodystrophy and induces insulin resistance in mice (He et al. (deepdyve.com)
  • NMR studies have tracked a correlation between OxPhos and insulin resistance in skeletal muscle. (bruker.com)
  • Ultrastructural analysis revealed that overexpression of CD97 strengthened lateral cell-cell contacts between enterocytes, which, in contrast, were weakened in CD97 knockout (Ko) mice. (nih.gov)
  • In contrast, mice were less sensitive to insulin during their inactive, fasting phase (sleep-state) , which is termed insulin resistance . (knowingneurons.com)
  • By contrast, male MARCH1 KO mice had similar weight gain and visceral adiposity to wildtype (WT) male mice. (arizona.edu)
  • We investigated the contribution of the p66Shc signaling pathway to diabetes-related cognitive decline using p66Shc knockout (−/−) mice. (nature.com)
  • In breast cancer, aberrations in the canonical Wnt signaling pathway have been found in correlation with poor prognosis. (unibas.ch)
  • p66Shc (−/−) diabetic mice also showed significant amelioration of cognitive dysfunction, as well as decreased levels of OS and inflammatory markers. (nature.com)
  • Future studies might use receptor-knockout mice to research the impacts of marijuana on inflammatory markers. (kingsilvernews.com)
  • The greatest ethnic differences in polymorphisms within the ABCC1 are found between Caucasian and Asian populations. (wikipedia.org)
  • isoforms Trichostatin-A supplier found in subcellular compartments including the cytoplasm (CyP-D, CyP-NK, CyP-40), endo(sarco)plasmic reticulum (CyP-B, CyP-C), nucleus (CyP-E), and mitochondria (CyP-D) (Lee and Kim, 2010). (mingsheng88.org)
  • Both isoforms exist in human and mouse pancreatic islets. (lu.se)
  • In fact, Cys203 residue of Cyp-D offers been shown to play a crucial part in oxidative stress induced activation of mPTP in mouse embryonic fibroblasts (Nguyen et al. (mingsheng88.org)
  • However, NOD1, NOD2, and Rip2 knockout mice were no more susceptible to infection with virulent B. abortus than wild-type mice. (hindawi.com)
  • Next, Ectopic expression of MINK1 efficiently rescued the 5FU resistance phenotypes in MINK1 KD (knock down) OSCC (Fig. 2C&D), which further depicts MINK1 dependency of 5FU resistance in OSCC. (springernature.com)
  • These mice were also resistant to diet-induced obesity and diabetes when fed a high fat diet. (bruker.com)
  • It actually made the mice resistant to the disease--a finding with potential implications for development of therapies for diabetes. (bruker.com)
  • p66Shc (−/−) mice have less OS in the brain and are resistant to diabetes-induced brain damage. (nature.com)
  • Still, treatment resistance inevitably develops and leads to an abysmal prognosis of stage-castration resistant PCa (CRPC). (tulane.edu)
  • By the numbers, treatment with colistin alone extended survival of almost 88 per cent of mice to approximately five days post infection and 25 per cent of mice survived to the end of the experiment. (newswise.com)
  • This study shows that a primary OxPhos defect does not cause T2DM in mice. (bruker.com)
  • Unexpectedly, we found a CD97 cDNA copy number-dependent reduction of DSS-induced colitis in Tg compared to wild-type (WT) mice that was confirmed by applying a simple DSS protocol. (nih.gov)
  • This mouse also showed an ectopic lipid in﫿ammation indicates that HFD induced adipocyte death would distribution and insulin resistance. (deepdyve.com)
  • The ideal result is an almost entirely Black 6 mouse with a 129 strain transgene. (the-scientist.com)
  • Specifically, 129 strain congenic mice on a Black 6 background that have been backcrossed for 10 generations have more than a 90 percent chance of having around 1 million base pairs of 129 strain DNA on either side of the transgene. (the-scientist.com)
  • Basically, they made the mice very inefficient consumers of fuel, which prevented them from gaining weight on a high fat diet or developing insulin resistance. (bruker.com)
  • To test whether disruptions in the sleep-awake cycle influenced body fat levels, wild-type mice were placed in a lit environment for three months, which completely disrupted their circadian cycles, and were fed a high-fat diet. (knowingneurons.com)
  • We discovered that the amounts and weights of individual MDA-MB-231 breast cancers xenografts were better in hyperinsulinemic mice weighed against handles (p 0.05), and Rabbit Polyclonal to SirT1 IR phosphorylation was 4.5-fold higher in these mice (p 0.05). (findadig.com)
  • Further, to understand the kinase driven mechanism regulating 5FU resistance, we performed a phosphorylation profiling using a phospho-antibody array in presence or absence of the target kinase. (springernature.com)
  • We found that the knockout of β-catenin and hence the loss of its signaling and adhesion function leads to apoptosis in vitro and in vivo. (unibas.ch)
  • Rrbp1 - knockout (KO) mice had lower blood pressure and were more likely to die suddenly from severe hyperkalemia caused by phenotypically hyporeninemic hypoaldosteronism than wild-type controls. (biomedcentral.com)
  • To mimic soft drinks, 10-week-old male wild-type (WT) and ghrelin knockout (Ghrelin-/-) mice were subjected to ad lib. (tamu.edu)
  • In this way, the mice had reduced insulin resistance, so insulin's actions were similar to wild-type mice, and they didn't gain any excess weight! (knowingneurons.com)
  • Mesenteric arterioles from wild type (WT) and SMTNL1 global knock-out (KO) mice were examined with pressure myography. (duke.edu)
  • That's because, according to a 1995 study and subsequent work, Casp1 knockout mice did not go into septic shock when challenged with molecules signaling foreign invaders. (the-scientist.com)
  • We noticed that the mice treated with antibiotics gained less weight than the mice that stayed in the old facility," said study co-first author Agnieszka Truax, PhD, a postdoctoral researcher in the Ting lab during the study. (integrativepractitioner.com)
  • In this study, we discovered that RRBP1 genetic variants were also associated with blood pressure in a large family-based genome-wide linkage and regional fine mapping of the Stanford Asia-Pacific Program for Hypertension and Insulin Resistance (SAPPHIRe) cohort. (biomedcentral.com)
  • In this study, mice were shown to be more sensitive to insulin during the high activity, feeding phase . (knowingneurons.com)
  • The goal of this study is to find out the potential kinase(s) those are major driver(s) of 5FU resistance in OSCC. (springernature.com)
  • The rise of antibiotic resistance has ushered in the post-antibiotic age, and alternatives to antibiotics are urgently required," said Caressa Tsai, first author of the study and a PhD student in biochemistry and biomedical sciences in the Coombes lab at McMaster. (newswise.com)
  • During normal development, RUNX3 is found to be expressed in the hematopoietic system, in osteoblasts and chondrocytes and in neurotrophin-positive neurons of the dorsal root ganglia [ 8 ] indicating its role in neuronal development [ 9 ]. (hindawi.com)
  • Mitochondria have been thought to contribute to insulin resistance, but their role has been unclear. (bruker.com)
  • They found that their interpretation of Casp3 's role in septic shock had also been confounded by the Casp11 passenger mutation. (the-scientist.com)
  • Herein, we have shown that NOD1, NOD2, or the adaptor molecule Rip2 plays no role in enhancing resistance to B. abortus infection in vivo . (hindawi.com)
  • We also explored a potential role for MARCH1 in human type 2 diabetes risk through genetic association testing in publicly-available datasets, and found evidence suggestive of association. (arizona.edu)
  • To specifically dissect its transcriptional function without affecting its role in cell adhesion, we have used mice expressing mutant forms of β-catenin which retained their function in cell adhesion but lacked either the N- or C-terminal transcriptional output or both, resulting in the abrogation of β-catenin's transcriptional activity and thus the canonical Wnt signaling output. (unibas.ch)
  • Consistently, we found that Rrbp1 - knockout (KO) mice had lower blood pressure and hyporeninemic hypoaldosteronism, which cause severe hyperkalemic cardiac arrhythmia-induced sudden death. (biomedcentral.com)
  • Drugs now used to treat insulin resistance can't reverse the progression to full-blown type-2 diabetes. (stanford.edu)
  • Remarkably, the knockout mice were also protected from excess weight gain when they were co-housed with control mice, hinting that "good" bacteria from the control mice were getting into them and helping to protect them. (integrativepractitioner.com)
  • We found that functional inhibition, genetic ablation, and inactivation by post-translational modification of OGG1. (lu.se)
  • The authors found that sites with the greatest inhibition of C. jejuni growth in vitro and the least colonization in vivo had the highest number of O-glycans. (thermofisher.com)
  • These mice appeared normal and healthy in every other respect, suggesting that Abcc10 is not essential for overall health and survival. (news-medical.net)
  • Such studies have also found that obesity tends to cluster within families, and that monozygotic twins show greater concordance in Body Mass Index (BMI) and adiposity metrics versus dizygotic twins. (biomedcentral.com)