Loading...
  • homogenates
  • The metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate in homogenates and sub-fractions from normal rat liver and premalignant liver nodules was investigated. (oup.com)
  • The activities of 5-phosphatase, expressed as pmol converted substrate per minute and mg protein, were equal when using the two substrates, and did not differ between normal and nodular homogenates. (oup.com)
  • The receptors were first identified as specific molecules through the use of binding studies, in which opiates that had been labeled with radioisotopes were found to bind to brain membrane homogenates. (wikipedia.org)
  • vitro
  • littermates, display an " activated" phenotype in vitro and in vivo and have an elevated level of cAMP phosphodiesterase (PDE) activity. (diabetesjournals.org)
  • Prior, in vitro studies have shown that reactive oxygen species (ROS) are involved in AO induced toxicity causing peroxidative damage of lipids in various hepatic sub-cellular fractions including microsomes and mitochondria of rats. (wikipedia.org)
  • A cell-free system is an in vitro tool widely used to study biological reactions that happen within cells apart from a full cell system, thus reducing the complex interactions typically found when working in a whole cell. (wikipedia.org)
  • Both the full chlorophyllase and the cleaved, mature chlorophyllase, however, experienced similar levels of activity in an in vitro assay. (wikipedia.org)
  • synaptic
  • Ultimately, substantial evidence was obtained to support a role for miRNA and the components of PTGS in the functional compartmentalisation of neurons and the response to activity, though further methodological developments are required to elaborate the novel mechanisms of miRNA function and investigate the direct contribution of miRNA-mediated PTGS to enabling real-time, activity-driven synaptic modification. (dissertation.com)
  • Whittaker's work demonstrating acetylcholine in vesicle fractions from guinea-pig brain was first published in abstract form in 1960 and then in more detail in 1963 and 1964, and the paper of the de Robertis group demonstrating an enrichment of bound acetylcholine in synaptic vesicle fractions from rat brain appeared in 1963. (wikipedia.org)
  • The isolation of highly purified fractions of cholinergic synaptic vesicles from the ray Torpedo electric organ was an important step forward in the study of vesicle biochemistry and function. (wikipedia.org)
  • cholinergic
  • ChAT is found in high concentration in cholinergic neurons, both in the central nervous system (CNS) and peripheral nervous system (PNS). (wikipedia.org)
  • The pChAT isoform was discovered in 2000 based on observations that brain-derived ChAT antibodies failed to stain peripheral cholinergic neurons as they do for those found in the brain. (wikipedia.org)
  • binding protein
  • The major Paraoxon-insensitive Mipafox-sensitive binding protein (Mr 160 000) was found to be identical with one previously identified as neurotoxic esterase, an enzyme that has been proposed to be the target site for organophosphorus-compound-induced delayed neurotoxicity. (cdc.gov)
  • nuclear
  • Addition of noradrenaline to reserpine-treated perfused hearts enhances, compared with the control, the incorporation of precursor into RNA in all subcellular fractions other than the nuclear one, restores incorporation of labelled putrescine into polyamines, enhances ornithine decarboxylase and S-adenosylmethionine decarboxylase activities and causes a 12-fold increase in cytoplasmic Mn2+-dependent polyadenylate polymerase activity. (biochemj.org)
  • liver
  • The compartmentalization of inositol polyphosphate 5-phosphatase activity presents a possible explanation of earlier findings that premalignant liver tissue was able to respond with inositol 1,4,5-trisphosphate, but not inositol 1,3,4,5-tetrakisphosphate formation after agonist stimulation. (oup.com)
  • The remainder of the administered dose was found throughout the rat, with it being found primarily in the digestive tract lumen and the liver. (wikipedia.org)
  • In addition, one study done in rats found the greatest effect of BPF to be liver toxicity. (wikipedia.org)
  • molecular
  • A protein with identical molecular weight was also recognized in immunoblots by a polyclonal antibody against serine protease (anti-SP), with higher immunoreactivity in the vesicular fraction. (scielo.br)
  • Subcellular fractions can be isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components. (wikipedia.org)
  • vivo
  • Interestingly, the distinction between these two compounds disappeared in vivo, where their cancer chemopreventive efficacies were found to be very similar to each other[ in both methylnitrosourea and dimethylbenz( a )anthracene rat mammary tumor models]. (aacrjournals.org)
  • A literature review of in vivo studies of BPF found that four out of five studies yielded results that BPF is estrogenic, androgenic and thyroidogenic. (wikipedia.org)
  • It has been shown that elevation of NPEPPS activity in vivo could effectively block accumulation of hyperphosphorylated TAU protein and thus slow down the disease progression, suggesting increasing NPEPPS activity may be a feasible therapeutic approach to eliminate accumulation of toxic substrates, which are involved in neurodegenerative diseases. (wikipedia.org)
  • rats
  • In pregnant rats, the BPF was also found in the uterus, placenta, amniotic fluid and fetuses, indicating that BPF is able to be absorbed into the reproductive tract and pass through the placental barrier in pregnant rats. (wikipedia.org)
  • kinase
  • Vasopressin (0.2 or 2 microM) had no effect either on phosphatase or kinase activity. (oup.com)
  • PAK1 contains an autoinhibitory domain that suppresses the catalytic activity of its kinase domain. (wikipedia.org)
  • Receptors for activated C-kinase (RACK) have been found to anchor active PKC in close proximity to substrates. (wikipedia.org)
  • bacteria
  • This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). (wikipedia.org)
  • citation needed] By repeatedly evaluating their course, and adjusting if they are moving in the wrong direction, bacteria can direct their motion to find favorable locations with high concentrations of attractants (usually food) and avoid repellents (usually poisons). (wikipedia.org)
  • citation needed] In other words, bacteria like E. coli use temporal sensing to decide whether their situation is improving or not, and in this way, find the location with the highest concentration of attractant (usually the source) quite well. (wikipedia.org)
  • Using a direct phase microscope, he examined the urine specimens of several patients with urinary tract infections and found atypical bacteria in his samples. (wikipedia.org)
  • cell
  • To investigate this in a human genetic context, techniques were established for isolating distinct subcellular fractions of the SH-SY5Y neuroblastoma cell line and examining genome-wide miRNA and mRNA responses to neuronal cues such as differentiation and depolarisation. (dissertation.com)
  • In the 1960s and 1970s, the revolution of modern cell biology and biochemistry provided a series of novel techniques that became available to investigate the migratory responder cells and subcellular fractions responsible for chemotactic activity. (wikipedia.org)
  • One day, the enzyme activity of purified cell fractions which had been refrigerated for five days was measured. (wikipedia.org)
  • Nobel prize winner Eduard Buchner was arguably the first to present a cell-free system using yeast extracts, but since then alternative sources have been found. (wikipedia.org)
  • phosphodiesterase
  • 1971). The specific distribution of cyclic AMP in various brain regions reflects the relative activities of the synthetic and degradative enzymes, adenyl cyclase, and phosphodiesterase. (springer.com)
  • pathway
  • Many of these proteins serve as adaptors that link p97/CDC48 to a particular subcellular compartment to function in a specific cellular pathway. (wikipedia.org)
  • The lower-energy production, per glucose, of anaerobic respiration relative to aerobic respiration, results in greater flux through the pathway under hypoxic (low-oxygen) conditions, unless alternative sources of anaerobically oxidizable substrates, such as fatty acids, are found. (wikipedia.org)
  • cellular
  • It was found out that Al-AT activities under impairment of feeding regime depend on several factors including morphofunctional peculiarities of the brain structure, terms of protein-free feeding, studied cellular substrates and animal age. (ac.ir)
  • Our data are consistent with an increased role for PDE3 in regulating cAMP-dependent signaling in cp / cp VSMCs and identify PDE3 as a cellular activity potentially responsible for the phenotype of cp / cp VSMCs. (diabetesjournals.org)
  • p97/CDC48 is a member of the AAA+ (extended family of ATPases associated with various cellular activities) ATPase family. (wikipedia.org)
  • Stimulation of PAK1 activity is accompanied by a series of cellular processes that are fundamental to living systems. (wikipedia.org)
  • At the biochemical level, these activities are regulated by the ability of PAK1 to phosphorylate its effector interacting substrates, which in-turn set-up a cascade of biochemical events cumulating into a cellular phenotypic response. (wikipedia.org)
  • identical
  • The recovery of the binding activity in the 160 000-Mr band was found to be similar to the recovery of neurotoxic esterase activity, lending further support to the contention that this band is identical with neurotoxic esterase. (cdc.gov)
  • proteins
  • However, two other binding proteins with suitable binding characteristics were also found in smaller amounts, one of which has not been detected previously. (cdc.gov)
  • Di-isopropyl phosphorofluoridate was found to phosphorylate all three of these proteins at rates similar to the rate at which neurotoxic esterase is inhibited by di-isopropyl phosphorofluoridate. (cdc.gov)
  • The virus proteins, reverse transcriptase (RT) and p30, were found to increase with time in the subcellular fractions of lymphocytic tissue from either the thymus or spleen of AKR mice with spontaneous lymphocytic leukaemia. (semanticscholar.org)
  • For example, p97 mutant proteins carrying single point mutations found in patients with IBMPFD (inclusion body myopathy associated with Paget disease of the bone and frontotemporal dementia) (see below) have 2-3fold increase in ATPase activity. (wikipedia.org)
  • These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. (wikipedia.org)
  • concentrations
  • The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. (ovid.com)
  • organisms
  • They are found in all organisms studied and participate in blood clotting and complement activation cascade reactions, phage maturation and fertilization, as well as any number of other fields of biological phenomena (Rawling & Barret 1994). (scielo.br)
  • Introns are found in the genes of most organisms and many viruses. (wikipedia.org)
  • cells
  • 2000 ) and found that these cells contained CYP2B1/2, 3 CYP2C11, CYP2E1, CYP4A2/3, and GSTα but not CYP3A1/2, GSTμ, or GSTπ. (aspetjournals.org)
  • Freshly isolated hPT cells were found to express high levels of certain P450s and GSH-dependent enzymes. (aspetjournals.org)
  • The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. (ovid.com)
  • In addition, another study found BPF to be genotoxic when introduced to Hep G2 cells. (wikipedia.org)
  • VMAT1 may be found in neuroendocrine cells, particularly chromaffin and enterochromaffin granules which are largely found in the medulla of the adrenal glands. (wikipedia.org)
  • genes
  • Two different genes encoding PDE3 activity have been identified, PDE3A and PDE3B , and each is expressed in rat and human VSMCs ( 20 , 21 ). (diabetesjournals.org)
  • hydrolysis
  • After treatment with noradrenaline the increase in radioactivity was found solely in AMP after hydrolysis of microsomal RNA to nucleoside monophosphates. (biochemj.org)
  • The lumen's pH (4.5-5.0) is optimal for the enzymes involved in hydrolysis, analogous to the activity of the stomach. (wikipedia.org)
  • The enzymes responsible for this hydrolysis require an acidic environment for optimal activity. (wikipedia.org)
  • acid
  • In an attempt to affirm the concept, the present study was designed to evaluate the activity of methylseleninic acid, a compound that represents a simplified version of Se-methylselenocysteine without the amino acid moiety, thereby obviating the need for β-lyase action. (aacrjournals.org)
  • To estimate this enzyme activity, they used that of standardised enzyme acid phosphatase, and found that the activity was only 10% of the expected value. (wikipedia.org)
  • Study
  • That study has been widely credited as the first definitive finding of an opioid receptor, although two other studies followed shortly after. (wikipedia.org)
  • different
  • However it was subsequently found that it shares little sequence similarity with the other opioid receptors, and has quite different function. (wikipedia.org)
  • various second messengers in different combinations can act on the C1 domain to direct subcellular translocation of PKCε. (wikipedia.org)
  • studies
  • These studies identified a pattern of activity-associated miRNA expression changes unique to the neurites that was revealed to be connected to the release of exosomes from this compartment. (dissertation.com)
  • domain
  • The two ATPase domains of p97 (D1 and D2) are not equivalent because the D2 domain displays higher ATPase activity than the D1 domain in wild-type protein. (wikipedia.org)
  • total
  • The main objective of this research is to identify SMase activity of T. vaginalis in the total extract (TE), P30, and S30 subfractions from brooked trophozoites. (hindawi.com)
  • mammalian
  • The coding sequence contains five regions showing a high degree of conservation in vertebrates, predominantly in exons 2, 5, 6, 7 and 8, but the sequences found in invertebrates show only distant resemblance to mammalian TP53. (wikipedia.org)
  • mice
  • Two lines of independently derived PrPC gene-ablated (Prnp0/0) mice exhibit severe reductions in the copper content of membrane-enriched brain extracts and similar reductions in synaptosomal and endosome-enriched subcellular fractions. (eurekamag.com)
  • As only a minute fraction of peripherally injected vaccines reaches the lymph nodes, vaccinations of mice and humans were performed by direct injection of vaccine into inguinal lymph nodes, i.e. intralymphatic injection. (jove.com)
  • D-leucine also terminates seizures in mice after the onset of seizure activity, at least as effectively as diazepam and without sedative effects. (wikipedia.org)
  • extracts
  • The protein was localized to the trans-Golgi compartment but also found in soluble extracts, leading the authors to propose that RGP1 is made on cytosolic polysomes and associates postsynthetically with Golgi membranes as a peripheral protein. (plantcell.org)
  • phospholipid
  • APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. (mybiosource.com)
  • Our studies indicate that the C-terminus of ATP8A2 plays an important role in promoting efficient protein folding and regulating phospholipid flippase activity. (pubmedcentralcanada.ca)
  • fatty
  • Hepatic lipogenic enzymes, such as fatty acid synthase (FAS), malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PDH) activities were inhibited by SSC treatment. (biomedsearch.com)
  • differential
  • A partially purified secretory granule fraction, isolated from rat islets of Langerhans by differential centrifugation, was used for investigating the stability of the beta granules during incubation in various conditions. (rupress.org)
  • concentration
  • ChAT is found in high concentration in cholinergic neurons, both in the central nervous system (CNS) and peripheral nervous system (PNS). (wikipedia.org)
  • This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). (wikipedia.org)
  • citation needed] In other words, bacteria like E. coli use temporal sensing to decide whether their situation is improving or not, and in this way, find the location with the highest concentration of attractant (usually the source) quite well. (wikipedia.org)
  • unclear
  • As these previous studies were performed in the absence of uncouplers that were recently found to influence PINK1 stability, it remains unclear how and where endogenous PINK1 is degraded. (rupress.org)
  • dependent
  • Nevertheless, their activities are dependent of each other. (wikipedia.org)
  • The human PAK1 was identified as a GTP-dependent interacting partner of Rac1 or Cdc42 in the cytosolic fraction from neutrophils, and its complementary DNA was cloned from a human placenta library by Martin and Colleagues in 1995. (wikipedia.org)
  • However
  • The solubilized protein, however, assembled with CDC50A and displayed phosphatidylserine flippase activity. (pubmedcentralcanada.ca)
  • However, albumin is lost at each process stage, with roughly 20% of the albumin lost through precipitation stages before fraction V. In order to purify the albumin, there is an extraction with water, and adjustment to 10% ethanol, pH of 4.5 at −3 °C. Any precipitate formed here is done so by filtration and is an impurity. (wikipedia.org)
  • Examples
  • While the recognition of gene sharing is relatively recent-the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes-recent studies have found many examples throughout the living world. (wikipedia.org)
  • mutant
  • A 2011 study of a Brazilian birth cohort found an association between the non mutant arginine TP53 and individuals without a family history of cancer. (wikipedia.org)
  • significant
  • Meta-analyses from 2011 found no significant associations between TP53 codon 72 polymorphisms and both colorectal cancer risk and endometrial cancer risk. (wikipedia.org)
  • availability
  • Argemone seeds find use as a substitute because of the easy availability, low cost and their complete miscibility of their oil with mustard oil. (wikipedia.org)
  • study
  • A study of Arab women found that proline homozygosity at TP53 codon 72 is associated with a decreased risk for breast cancer. (wikipedia.org)
  • A 2011 study found that TP53 codon 72 polymorphism was associated with an increased risk of lung cancer. (wikipedia.org)
  • Another 2011 study found that the p53 homozygous (Pro/Pro) genotype was associated with a significantly increased risk for renal cell carcinoma. (wikipedia.org)
  • liquid
  • The fibrinogen and thrombin fractions were further combined during the War into additional products, including liquid Fibrin Sealant, solid Fibrin Foam and a Fibrin Film. (wikipedia.org)
  • higher
  • The pH is 5.2 and it is conducted at −5 °C. Several variations of Cohn fraction were created to account for lower cost and higher yield. (wikipedia.org)
  • another
  • Another use for BPF is in dental materials, where it can be found in restorative materials, liners, adhesives, oral prosthetic devices and tissue substitutes. (wikipedia.org)