• Mice
  • Mice that are deficient in claudin-2 have reduced reabsorption of Na+ in the proximal tubule, consistent with a role in paracellular transport. (wikipedia.org)
  • Increased fatty acid (FA) synthesis enzymes and triglyceride deposition correlated with increased profibrotic factors were found in the kidney in diabetes in rats ( 4 ) and mice ( 5 ). (diabetesjournals.org)
  • kidney's
  • Co-first authors of the study Kimberly Homan, Ph.D., a Wyss Research Associate, and David Kolesky, Ph.D., a Wyss Postdoctoral Fellow, stress that the most exciting aspect of the work is that - far beyond mimicking the form of the kidney's proximal tubule - it is a credible in vitro model that functions like living kidney tissue, representing a significant advance from traditional 2D cell culture. (harvard.edu)
  • Type IV renal tubular acidosis (aldosterone resistance of the kidney's tubules) Gordon's syndrome (pseudohypoaldosteronism type II) ("familial hypertension with hyperkalemia"), a rare genetic disorder caused by defective modulators of salt transporters, including the thiazide-sensitive Na-Cl cotransporter. (wikipedia.org)
  • chronic
  • Indeed, advances in biomarker discovery and a greater appreciation of tubulointerstitial histopathology and the role of tubular hypoxia in the pathogenesis of chronic kidney disease have given us pause to reconsider the current "glomerulocentric" paradigm and focus attention on the proximal tubule that by virtue of the high energy requirements and reliance on aerobic metabolism render it particularly susceptible to the derangements of the diabetic state. (diabetesjournals.org)
  • Understanding of the causes of CAKUT and the mechanisms underlying disease progression towards chronic kidney disease (CKD) and ESKD is essential for prediction of prognosis. (clinicaladvisor.com)
  • cellular
  • The evidence of cellular change of mouse stem cells in response to a rat scaffold is a promising step toward establishment of a xenogenic scaffold source for engineered kidneys. (ufl.edu)
  • The data show that our model could reproduce ischemically induced cell swelling and characterized the response at the cellular level of tubules to different components of preservation solutions. (ebscohost.com)
  • Agonal resorption of the proximal tubular contents after interruption of circulation in the capillaries surrounding the tubule often leads to disturbance of the cellular morphology of the proximal tubule cells, including the ejection of cell nuclei into the tubule lumen. (wikipedia.org)
  • sodium
  • The alpha-ketoglutarate generated in this process is then further broken down to form two bicarbonate anions, which are pumped out of the basolateral portion of the tubule cell by co-transport with sodium ions. (wikipedia.org)
  • lateral
  • In vertebrates, tight junctions (TJs) are found at the apical-most portion of the lateral surfaces, where the TJs form barriers both between the apical and basolateral surfaces and between adjacent cells, limiting paracellular permeability 7 ( FIG. 1a ). (pubmedcentralcanada.ca)
  • glomerular
  • These findings are, however, juxtaposed with the more recent knowledge that some patients with advanced disease display neither substantial glomerular pathology nor proteinuria and that kidney function declines well before traditional indicators of kidney disease such as microalbuminuria or creatinine-based estimated glomerular filtration rate (eGFR) decline ( 2 ). (diabetesjournals.org)
  • Moreover, the diabetes-induced injury to the proximal tubule may in turn lead to glomerular pathology and postglomerular hypoperfusion, while fibrotic expansion of the interstitium compresses and further disrupts the local microvasculature. (diabetesjournals.org)
  • Cells delivered into the renal artery showed initial glomerular localization with subsequent expansion into adjacent vasculature, interstitium, and tubules. (ufl.edu)
  • Renal tubular acidosis (proximal type) (Fanconi syndrome) occurs when the PTECs are unable to properly reabsorb glomerular filtrate so that there is increased loss of bicarbonate, glucose, amino acids, and phosphate. (wikipedia.org)
  • functional
  • The current work further expands our bioprinting platform to create functional human tissue architectures with both technological and clinical relevance," said Lewis, who is also the Hansj√∂rg Wyss Professor of Biologically Inspired Engineering at the Harvard John A. Paulson School of Engineering and Applied Sciences. (harvard.edu)
  • In this video, see how the Wyss Institute team has advanced bioprinting to the point of being able to fabricate a functional subunit of a kidney, as reported in a new study published in Scientific Reports. (harvard.edu)
  • The use of functional tissue-like models during pre-clinical studies will provide unprecedented insights into human-relevant drug response prior to clinical development," said Moisan, a Laboratory Head in Mechanistic Safety at Roche and author of this study. (harvard.edu)
  • Human proximal tubule cells adhere to the hollow channel, forming a functional, 3D renal architecture. (harvard.edu)
  • urinary
  • Congenital anomalies of the kidney and urinary tract (CAKUT) occur in 1 in 500 births, account for the most cases of pediatric end-stage kidney disease (ESKD), and predispose an individual to hypertension and cardiovascular disease throughout life. (clinicaladvisor.com)
  • Increases in serum and urinary GST-A1 have been found in association with hepatocyte and renal proximal tubular necrosis respectively and have potential for monitoring injury to these tissues. (wikipedia.org)
  • subunit
  • Therefore, the bioprinted 3D renal architecture recapitulates a very small - yet critical - subunit of a whole kidney. (harvard.edu)
  • While thus far we have merely demonstrated a functioning subunit within the kidney, we are actively scaling up the method and its complexity to enable future in vivo applications. (harvard.edu)
  • transplant
  • Everolimus permits reduced calcineurin inhibitor (CNI) exposure, but the efficacy and safety outcomes of this treatment after kidney transplant require confirmation. (bioportfolio.com)
  • The purpose of this study was to identify the risk factors for everolimus discontinuation in kidney transplant recipients converted to everolimus with calcineurin inhibitor (CNI) minimization at a lat. (bioportfolio.com)
  • It is used to prevent GRAFT REJECTION in heart and kidney transplant patients by blocking cell proliferation signals. (bioportfolio.com)
  • differentiate
  • Examining the ability of these cells to proliferate and differentiate in a whole kidney extracellular matrix scaffold would serve as a valuable baseline for future studies in producing a functioning organ. (ufl.edu)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
  • mechanisms
  • To date, the mechanisms responsible for these kidney regeneration phenomena remain poorly understood. (jove.com)
  • regeneration
  • The field has aquired a good general base of knowledge, but scrutiny of the techniques and outcomes will be necessary to move toward a unified explanation of renal regeneration and the role of organ-specific stem cells in the kidney. (biomedcentral.com)
  • Tissue engineering is the development of biological substitutes and/or the fostering of tissue regeneration/remodelling. (ebscohost.com)
  • The zebrafish adult kidney is an excellent system for renal regeneration and disease studies. (jove.com)
  • The zebrafish model has emerged as a relevant system to study kidney development, regeneration and disease. (jove.com)
  • adult
  • Tissue engineering and regenerative medicine research seeks better treatment alternatives through the application of adult renal stem cells. (biomedcentral.com)
  • Stem cells sourced from the adult kidney have the potential benefits of improved renal engraftment and differentiation and can be utilized in autologous therapies. (biomedcentral.com)
  • commonly
  • This study will see how these two commonly used treatments (Everolimus and Panobinostat) work together in treating kidney cancer. (bioportfolio.com)
  • disease
  • Are you sure your patient has congenital anomalies/polycystic kidney disease? (clinicaladvisor.com)
  • Physicians taking a medical history may focus on kidney disease, medication use (e.g. potassium-sparing diuretics), which are common causes. (wikipedia.org)
  • genes
  • Animal experiments demonstrate that alterations in genes critical for kidney development can cause experimental CAKUT. (clinicaladvisor.com)
  • The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver (hepatocytes) and kidney (proximal tubules). (wikipedia.org)
  • cells
  • This work builds upon their demonstrated ability to bioprint tissue constructs composed of multiple types of living cells patterned alongside a vascular network in an extracellular matrix. (harvard.edu)
  • Lewis' team achieved this advance by adapting their earlier approach for bioprinting living cells to form thick tissues. (harvard.edu)
  • Although almost every eukaryotic cell is spatially asymmetric or polarized, polarity must be coordinated in space and time for individual cells to form a tissue 1 . (pubmedcentralcanada.ca)
  • the emergent properties of the tissue result from the combined roles of the individual cells that are involved. (pubmedcentralcanada.ca)
  • Accordingly, the best studied polarized tissue is the simple epithelium of the mammalian intestine and kidney, the cells of which are columnar in shape (that is, they are taller than they are wide). (pubmedcentralcanada.ca)
  • In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikipedia.org)
  • organ-specific
  • Members of the claudin protein family, such as CLDN2, are expressed in an organ-specific manner and regulate the tissue-specific physiologic properties of tight junctions (Sakaguchi et al. (wikipedia.org)
  • found
  • In 1895 Thomas Morgan removed one of a frog's two blastomeres and found that amphibians are able to form whole embryos from the remaining part. (wikipedia.org)
  • whole
  • Decellularization of whole rat kidneys was achieved by perfusion of detergent-based solutions through the renal artery. (ufl.edu)
  • Our novel analysis forms the basis of future whole-organ studies that ultimately may allow us to propose an optimum platform for improved preservation solutions. (ebscohost.com)
  • distinct
  • Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. (wikipedia.org)