ImmunohistochemistryMicroarrayParaffin embeddedImmunohistochemicalAzideHypermethylationLesionsHistological analysisMicroarraysSpecimensTherapeuticProteinsMolecularMalignantMalignanciesPathwaysExpressed genesPeripheral nervouTestisPromoterQPCRFrozenHumanClinicalMechanismProtein expressionExpressionCellsResultsBrainFoundCellResearchMouseSignalsLevelsHighOrigin
Immunohistochemistry8
- this array can help scientists high throughput analyze gene and protein expressions in multiple tissue samples, especially analyze different protein levels with antibodies by immunohistochemistry. (wikipedia.org)
- Tests commonly employed in frozen tissue array include immunohistochemistry, and in situ hybridization. (wikipedia.org)
- 18 With the exception of the ovarian neoplasms, the same carcinomas analyzed for gene expression were also examined for HE4 by immunohistochemistry on tissue microarrays (see below). (nature.com)
- Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Mouse thyroid tissue section stained with Thyroglobulin Antibody (2H11 + 6E1). (novusbio.com)
- Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. (novusbio.com)
- Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. (novusbio.com)
- Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). (novusbio.com)
- Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. (novusbio.com)
Microarray8
- Cytomics Frozen section procedure MicroArray and Gene Expression (MAGE) Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. (wikipedia.org)
- Schoenberg Fejzo M, Slamon DJ: Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. (wikipedia.org)
- To identify miRNAs that potentially cause the resistance to taxanes, such as PTX, we performed microarray analysis of miRNAs in KFr13 and PTX-resistant KFr13Tx cells. (nature.com)
- Here, we examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. (nature.com)
- In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. (frontiersin.org)
- A tissue microarray was used to study EDN3 protein expression in breast carcinoma (n = 150) and normal breast epithelium (n = 44). (biomedcentral.com)
- In this study, we tried to identify differentially expressed genes between largest (F1) and second-largest follicles (F2), and classify them by global gene expression profiling using a combination of microarray and quantitative real-time PCR (QPCR) analysis. (biomedcentral.com)
- Hierarchical cluster analysis of microarray data classified the follicles into two clusters. (biomedcentral.com)
Paraffin embedded2
- Most of the arrays can be easily made by microarraying instrument (Beecher Instruments Inc.). However, paraffin embedded tissues have limitations. (wikipedia.org)
- Since frozen tissue sections don't go through any fixation procedures, and therefore, the DNA, RNA, and protein in frozen tissues retain their native characteristics much more than in paraffin embedded tissues. (wikipedia.org)
Immunohistochemical1
- Immunohistochemical analysis showed increased FOXP3, IFN-γ, CD1a, and CD4 expression in CA than in the control tissue samples. (bvsalud.org)
Azide4
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. (neobiotechnologies.com)
- 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. (novusbio.com)
- Antibody with azide - store at 2 to 8C. (novusbio.com)
- Antibody without azide - store at -20 to -80C. (novusbio.com)
Hypermethylation2
- Furthermore, EDN3 promoter hypermethylation was detected in 70% of primary breast carcinomas with significant association to loss of EDN3 mRNA expression ( P = 0.005), whilst normal matched breast tissues revealed no EDN3 promoter methylation. (biomedcentral.com)
- Here we have investigated nc886 in gastric cancer where its expression is suppressed due to CpG DNA hypermethylation at its promoter region in a cohort of paired tumor/normal tissues from 88 gastric cancer patients. (oncotarget.com)
Lesions2
- Gene expression analysis revealed 31 differentially expressed genes in CA lesions. (bvsalud.org)
- The system of Bethesda is a classification's system that allows an excellent interaction between the doctor and the pathologist in the analysis of cytopathologics cervicovaginals' smears, resulting from neoplasics and viral lesions, caused by the papilomavírus. (ndltd.org)
Histological analysis3
- Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to allow simultaneous histological analysis. (wikipedia.org)
- Sections from this block are cut using a cryostat, mounted on a microscope slide and then analyzed by any method of standard histological analysis. (wikipedia.org)
- The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
Microarrays3
- Tissue microarrays from frozen tissues-OCT technique. (wikipedia.org)
- Using tissue microarrays and full tissue sections of normal and 448 neoplastic tissues, HE4 immunoreactivity was found in normal glandular epithelium of the female genital tract and breast, the epididymis and vas deferens, respiratory epithelium, distal renal tubules, colonic mucosa, and salivary glands, consistent with HE4 gene expression. (nature.com)
- Here, we used oligonucleotide and tissue microarrays to survey HE4 gene and protein expression in a large group of malignant and normal tissues from adults. (nature.com)
Specimens1
- EDN3 mRNA expression was assessed by Northern blotting in normal human tissues (n = 9) as well as in matched pairs of normal and tumourous tissues from breast specimens (n = 50). (biomedcentral.com)
Therapeutic2
- Scientists who have been developing antibodies for therapeutic purpose all need their preliminary results from frozen tissues to get approval from FDA. (wikipedia.org)
- In particular, the present invention provides neurogenesis inducing genes coding for Zic proteins, vectors containing such genes, host cells containing such vectors, proteins produced by such host cells, antibodies raised to such proteins, and therapeutic agents or agents for gene therapy for nervous diseases. (justia.com)
Proteins2
- Buffered formalin solutions cross link proteins and nucleic acids when they are used for fix tissues. (wikipedia.org)
- Analysis of Protein Array containing more than 19,000 full-length human proteins using CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (neobiotechnologies.com)
Molecular1
- Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
Malignant1
- however, the expression in normal and malignant adult tissues has not been examined in detail. (nature.com)
Malignancies1
- Cutaneous tumors encompass a vast array of malignancies, ranging from neuroendocrine to lymphoid in origin. (medscape.com)
Pathways3
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
- In particular, PBPK models require quantitative information on the levels and activity of individual pathways involved in drug disposition across different tissues and populations (healthy vs. diseased or children vs. adults). (njacs.org)
- The immunomarkers were chosen based on the evaluation of the innate and adaptive immune pathways using qPCR analysis of 92 immune-related genes, applying a TaqMan Array Immune Response assay in HPV 6 or HPV 11 positive samples (n = 27). (bvsalud.org)
Expressed genes1
- Transcriptomic analysis on 72 h-exposed fetal testis explants revealed 187 differentially expressed genes (DEGs), including genes involved in steroid synthesis and toxic substance response. (biomedcentral.com)
Peripheral nervou2
- Leprosy (Hansen disease), a chronic infectious disease caused by Mycobacterium leprae , primarily affects the peripheral nervous system and involves skin and other tissues ( 1 ). (cdc.gov)
- A balanced regulation of this EDNRA/EDNRB interplay - also referred to as the endothelin axis (ET-axis)- is essential for, for example, homing processes to tissue destinations, where cells differentiate into numerous lineages such as the peripheral nervous system, structural and connective tissue components, cardiac cells or pigment-producing melanocytes [ 9 ]. (biomedcentral.com)
Testis1
- Depending on the molecules and testis age, highly deleterious effects of phytocannabinoid exposure were observed on testis tissue after 14 days, including Sertoli and germ cell death. (biomedcentral.com)
Promoter1
- EDN3 promoter methylation was analysed by methylation-specific PCR in breast cell lines (n = 6) before and after demethylating treatment, normal breast tissues (n = 17) and primary breast carcinomas (n = 128). (biomedcentral.com)
QPCR1
- Global gene expression profiles of F1 (10.7 +/- 0.7 mm) and F2 (7.8 +/- 0.2 mm) were analyzed by hierarchical cluster analysis and expression profiles of 16 representative genes were confirmed by QPCR analysis. (biomedcentral.com)
Frozen4
- Consequently, frozen tissue array should be the best tool for high throughput analysis on this purpose. (wikipedia.org)
- Frozen tissue cores with 2 mm diameter from the regions of interest are removed from frozen tissue OCT blocks at different freezing temperature since each tissue type has their own temperature preference at frozen stage. (wikipedia.org)
- Then all the frozen tissue cores are inserted in a recipient OCT frozen block in a precisely spaced, array pattern. (wikipedia.org)
- Each frozen tissue array block can be cut into 100-500 sections, which can be subjected to independent tests. (wikipedia.org)
Human2
- Here, we aimed at a first comprehensive analysis on EDN3 expression and its implication in human breast cancer. (biomedcentral.com)
- Although non-CYP enzymes are expressed in multiple human tissues, differential tissue expression and interindividual variability in the expression of these enzymes are not well studied. (njacs.org)
Clinical1
- 459 Hughes Drive Traverse City, MI 49686 Accutest Research Laboratories offers clinical trials, method development, pharmacokinetic analysis, BA/BE studies, and herbal medicine screening. (contractresearchmap.com)
Mechanism2
- We investigated the mechanism of chemoresistance by analysis of microRNAs using the ovarian cancer cell line KFr13 and its PTX-resistant derivative (KFr13Tx). (nature.com)
- Among other attributes, successful ADCs optimize targeting antibody, conjugation chemistry, and payload mechanism of action. (aacrjournals.org)
Protein expression1
- High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. (frontiersin.org)
Expression3
- We found RCC tissues displayed significantly higher miR-34a expression level than their corresponding noncancerous tissues, particularly in chromophobic subtype. (hindawi.com)
- MIR-34A has its own transcript and is expressed at higher levels than MIR-34B/C in most tissues, and this expression could be dysregulated in multiple diseases, especially in cancers [ 11 ]. (hindawi.com)
- Using RNA sequencing (RNA-seq) data from primary and xenograft SCLC samples, we identified seizure-related homolog 6 (SEZ6) as a surface-expressed SCLC target with broad expression in SCLC and minimal normal tissue expression by both RNA-seq and IHC. (aacrjournals.org)
Cells3
- In this presentation, we will discuss the development of in situ/vivo approaches by cellular and subcellular mass spectrometry that enabled our lab to determine the proteomic and metabolomic profile of identified cells in live Xenopus laevis frog embryos developing to tadpoles and neurons in mouse brain tissues. (njacs.org)
- Flow Cytometric Analysis of PFA-fixed U87MG cells. (neobiotechnologies.com)
- Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. (novusbio.com)
Results1
- Therefore, many scientific experimental results from formalin fixed tissues are not reliable. (wikipedia.org)
Brain1
- The ECS, which is sensitive to phytocannabinoids, plays a fundamental physiological role in the brain and peripheral tissues, as well as in many pathological conditions [ 14 ]. (biomedcentral.com)
Found1
- Through biological methods and immunofluorescence analysis both in vivo and in vitro , we found that this improvement was attributable in part to the clearance of M1 macrophages populations and the increase in M2 subtypes with the change of macrophage-mediated factors. (ijbs.com)
Cell1
- With antikeratin antibodies, the clear cell acanthomas stain for AE1 and AE3, but did not stain for CAM5.2. (medscape.com)
Research1
- This antibody is available for research use only and is not approved for use in diagnosis. (neobiotechnologies.com)
Mouse1
- SDS-PAGE Analysis Purified CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5). (neobiotechnologies.com)
Signals2
- We used a custom, fast tissue-clearing protocol based on a high refractive-index aqueous solution to increase the imaging depth and allow us the detection of faint signals and we characterized the autofluorescence (AF) from the various cellular and sub-cellular components of the ENS. (frontiersin.org)
- Z-scores are described in units of standard deviations (SD s) above the mean value of all signals generated on that array. (neobiotechnologies.com)
Levels1
- The DNA, RNA, and protein within the tissues are damaged in various levels during the fixation. (wikipedia.org)
High1
- ROC analysis showed high diagnostic performance of miR-34a (AUC = 0.854), MET (AUC = 0.765), and E2F3 (AUC = 0.761). (hindawi.com)
Origin1
- Adenocarcinomas of other-than-thyroid origin do not react with this antibody. (novusbio.com)