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  • reagents
  • Optimal staining depends on a number of factors including the antibody dilution, the staining chemicals, the preparation and/or fixation of the cells/tissue, and length of incubation with antibody/staining reagents. (wikipedia.org)
  • Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product. (wikipedia.org)
  • inhibition
  • Consistent with a role for the KKXX retrieval motif found at the cytoplasmic carboxyl terminus of p53/58 in retrograde traffic, inhibition of transport through VTCs correlates with the ability of the antibody to block recruitment of COPI coats to the p53/58 cytoplasmic tail and to p53/58-containing membranes. (nih.gov)
  • Inset) Antibody inhibition precedes the Ca2+-dependent fusion of VTCs to the Golgi stack. (nih.gov)
  • Viral hemagglutination inhibition tests for antibodies by the antibodies' ability to prevent viruses from agglutinating RBCs. (slideserve.com)
  • cells
  • The antitail antibody blocks transport before the delivery of cargo to the Golgi compartment containing α-1,2 mannosidase I. Semiintact CHO clone 15B cells were incubated at 32°C in a complete transport cocktail for indicated time in the absence (closed circles) or presence (open circles) of antitail antibody. (nih.gov)
  • 1994). The cells were pelleted, resuspended in a transport cocktail that contained either 5 mM EGTA (a), 0.1 μM Ca2+ (b), or 0.1 μM Ca2+ and 10 μg of antitail antibody (c), and incubated at 32°C for 90 min. (nih.gov)
  • As shown in Fig. 4 (closed circles), semiintact CHO 15B cells incubated in the absence of antibody at 32°C processed VSV-G to the Man5 form. (nih.gov)
  • This technique gives a picture of the protein-DNA interactions that occur inside the nucleus of living cells or tissues. (wikipedia.org)
  • Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. (wikipedia.org)
  • glycoprotein
  • Here we present evidence that this antibody arrests the anterograde transport of vesicular stomatitis virus glycoprotein and leads to the accumulation of p58 in pre-Golgi intermediates. (nih.gov)

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  • immunoperoxidase
  • In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction. (wikipedia.org)
  • (wikipedia.org)
  • In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining). (wikipedia.org)
  • procedure
  • The direct attachment of the messenger to the antibody reduces the number of steps in the procedure, saving time and reducing non-specific background signal. (wikipedia.org)
  • Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. (wikipedia.org)
  • IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). (wikipedia.org)
  • DAPI
  • A number of primary megakaryocytes treated with TPO were stained with DAPI, anti-α-tubulin antibody, anti-γ-tubulin antibody, anticentriole antibody, or anticentromere antibody. (nih.gov)
  • A) Megakaryocyte in prophase was stained with DAPI (a), anti-α-tubulin antibody (b), anticentriole antibody (c), or all three (d). (nih.gov)
  • B) Megakaryocyte in prometaphase was probed with DAPI (a), anti-α-tubulin antibody (b), anti-γ-tubulin antibody (c), or all three (d). (nih.gov)
  • C and D) Megakaryocytes in metaphase, with centrosomes numbering four (C) and eight (D), were stained with DAPI (a), anti-α-tubulin antibody (b), anticentriole antibody (c), or all three (d). (nih.gov)
  • E) Megakaryocyte in anaphase A was stained with DAPI (a), anti-α-tubulin antibody (b), anticentromere antibody (c), or all three (d). (nih.gov)
  • Diagnosis
  • By using a known database of previously catalogued organisms, similar techniques can produce results that reference back to this database, resulting in rapid diagnosis of microorganisms. (wikipedia.org)
  • enzyme
  • To localize the step in transport sensitive to antibody, we made use of the CHO clone 15B cell line, which lacks the cis/medial-Golgi enzyme N-acetylglucosamine-transferase I (GlcNAC Tr I) (Tabas and Kornfeld, 1979). (nih.gov)
  • The primary antibody can be directly tagged with the peroxidase enzyme which is then used to catalyse a chemical reaction to generate a coloured product. (wikipedia.org)
  • The secondary antibody is tagged with the peroxidase enzyme. (wikipedia.org)
  • cellular
  • In laboratory research, antibodies against specific markers of cellular differentiation can be used to label individual cell types. (wikipedia.org)
  • By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out of cellular lysates. (wikipedia.org)
  • Direct
  • Other catalytic enzymes such as alkaline phosphatase can be used instead of peroxidases for both direct and indirect staining methods. (wikipedia.org)
  • cell
  • Chondrocyte mechanical properties were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. (duke.edu)

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  • microscopy
  • Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. (jove.com)
  • Genetic combinatorial marking using lentiviral vectors encoding fluorescent proteins (FPs) enabled cell fate mapping through advanced microscopy imaging. (jove.com)
  • Core C will centralize all mice-related work across all three projects of this program, including generating new strains, breeding and housing of mice, generating preclinical VILI/ARDS and VILI+LPS models, accessing therapeutic effects of siRNAs and chemicals, performing lung inflammation assessment, and providing tissue samples and freshly dissociated murine lung vascular endothelial cells to each project for specific assays (including immunohisto- and immunocytochemistry, western blot analysis, fluorescent microscopy). (grantome.com)
  • Plates are subsequently probed with fluorescently labeled antibodies against a viral antigen, and fluorescence microscopy is used to count and quantify the number of foci. (wikipedia.org)
  • These phenomena are usually visualized using the laboratory techniques of staining and fluorescence microscopy. (wikipedia.org)
  • Chromatin bridges can be viewed utilizing a laboratory technique known as fluorescence microscopy. (wikipedia.org)
  • As with all types of fluorescence microscopy, the correct absorption wavelength needs to be determined in order to excite the fluorophore tag attached to the antibody, and detect the fluorescence given off, which indicates which cells are positive for the presence of the virus or bacteria being detected. (wikipedia.org)
  • The attached fluorophore can be detected via fluorescent microscopy, which, depending on the messenger used, will emit a specific wavelength of light once excited. (wikipedia.org)
  • Due to its tight and selective binding to F-actin, derivatives of phalloidin containing fluorescent tags are used widely in microscopy to visualize F-actin in biomedical research. (wikipedia.org)
  • Related techniques are also useful in sub-typing lymphocytes which all look quite similar on light microscopy. (wikipedia.org)
  • recognize
  • This study demonstrated that the recombinant anti-intimin antibody obtained is able to recognize the conserved region of intimin (Int 388-667 ) in purified form and the EPEC isolate. (biomedcentral.com)
  • There have been efforts in epitope mapping since many antibodies can bind the same epitope and levels of binding between antibodies that recognize the same epitope can vary. (wikipedia.org)
  • covalently
  • However, the direct method is far less practical than the indirect method, and is not commonly used in laboratories, since the primary antibodies must be covalently labeled, which require an abundant supply of purified antibody. (wikipedia.org)
  • The hapten is covalently linked to the primary antibody by succinyl imidesters or conjugated IgG Fc-specific Fab sections. (wikipedia.org)
  • specific
  • This will include quantitative analysis of the size of specific cell populations (identified by chromagenic or fluorescent antibody staining) in a specified anatomic location (e.g., the frequency of macrophages in the parafollicular T cell zone). (grantome.com)
  • Specific Aim #5 will evaluate the function of ARDS- associated single nucleotide polymorphisms (SNPs) and sites of functional protein post-translational modification (PTM), utilizing mutated cDNA (high efficiency expression plasmids) targeting the lung endothelium (with ACE antibody-conjugated liposome) in the endothelial conditional knockout mice. (grantome.com)
  • Immunocytochemistry with a 5-HT 1A -specific antibody demonstrated that subsets of TRCs were immunopositive for 5-HT 1A . (physiology.org)
  • A homogeneous immunoassay method and system for quantitative determination of total immunoglobulin E and specific antibody levels to a plurality of allergens, in which a relatively small sampling of blood is required. (patentsencyclopedia.com)
  • 8. The system of claim 1, wherein the allergen-specific antibodies from the patient's serum sample are allergen-specific IgE antibodies, and the anti-human antibody in the first binding agent is an anti-human IgE antibody. (patentsencyclopedia.com)
  • During the manufacture of these antibodies, antigen specific antibodies are sequestered by attaching the antigenic peptide to an affinity column and allowing nonspecific antibody to simply pass through the column. (wikipedia.org)
  • Hence, the specificity of the antibody is established by the specific reaction with the protein or peptide that is used for immunization by specific methods, such as immunoblotting or immunoprecipitation. (wikipedia.org)
  • This technique can be used to quickly determine if a subject has a specific viral or bacterial infection. (wikipedia.org)
  • The direct attachment of the messenger to the antibody reduces the number of steps in the procedure, saving time and reducing non-specific background signal. (wikipedia.org)
  • Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. (wikipedia.org)
  • In laboratory research, antibodies against specific markers of cellular differentiation can be used to label individual cell types. (wikipedia.org)
  • The protein of interest is isolated with a specific antibody. (wikipedia.org)
  • amyloid
  • The name amyloid comes from the early mistaken identification by Rudolf Virchow of the substance as starch (amylum in Latin, from Greek ἄμυλον amylon), based on crude iodine-staining techniques. (wikipedia.org)
  • substantially
  • d) means for substantially simultaneously detecting changes in fluorescent emission by the target species through the annular wall of each capillary. (google.ca)
  • direct
  • Next to direct transmission from person-to-person also indirect transmission from contaminated surfaces is well documented and important route of infections. (beds.ac.uk)
  • The BAD shall hold responsibility for the accuracy of the information published, and neither the scheme operator nor the scheme owner shall have any responsibility for costs, losses, or direct or indirect damages or costs arising from inaccuracy of information or omissions in information published on the website on behalf of the BAD. (bad.org.uk)
  • There are two methods involved in immunolabeling, the direct and the indirect methods. (wikipedia.org)
  • Also, the direct method is potentially far less sensitive than the indirect method. (wikipedia.org)
  • Other catalytic enzymes such as alkaline phosphatase can be used instead of peroxidases for both direct and indirect staining methods. (wikipedia.org)
  • method
  • Depending on the method of fixation and tissue preservation, the sample may require additional steps to make the epitopes available for antibody binding, including deparaffinization and antigen retrieval. (wikipedia.org)
  • Combining with other new techniques, this method can be used to screen protein-protein interactions and their modulators, DERB. (wikipedia.org)
  • However, this method also depends on the availability of suitable antibodies. (wikipedia.org)
  • tissue
  • This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized. (wikipedia.org)
  • Thus
  • Thus, as these TRCs are not directly connected with the afferent nerve, they must, when stimulated by tastants, utilize indirect mechanisms to produce an afferent neural discharge. (physiology.org)