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  • reagents
  • Optimal staining depends on a number of factors including the antibody dilution, the staining chemicals, the preparation and/or fixation of the cells/tissue, and length of incubation with antibody/staining reagents. (wikipedia.org)
  • Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product. (wikipedia.org)

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  • immunoperoxidase
  • In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction. (wikipedia.org)
  • (wikipedia.org)
  • In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining). (wikipedia.org)
  • cells
  • Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. (wikipedia.org)
  • procedure
  • The direct attachment of the messenger to the antibody reduces the number of steps in the procedure, saving time and reducing non-specific background signal. (wikipedia.org)
  • Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. (wikipedia.org)
  • IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). (wikipedia.org)
  • cellular
  • In laboratory research, antibodies against specific markers of cellular differentiation can be used to label individual cell types. (wikipedia.org)
  • By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out of cellular lysates. (wikipedia.org)
  • Direct
  • Other catalytic enzymes such as alkaline phosphatase can be used instead of peroxidases for both direct and indirect staining methods. (wikipedia.org)
  • cell
  • Chondrocyte mechanical properties were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. (duke.edu)
  • sample
  • This can be seen in that it is rarely possible to precipitate even half of a given protein from a sample with a single antibody, even when a large excess of antibody is used. (wikipedia.org)
  • Depending on the method of fixation and tissue preservation, the sample may require additional steps to make the epitopes available for antibody binding, including deparaffinization and antigen retrieval. (wikipedia.org)