• This approach is easily incorporated into most existing phagocytosis assays and allows for reproducible results with high sensitivity. (lu.se)
  • These assays usually require labeling each antibody with a fluorescent tag, and in nearly all of these cases the labeled antibody must then be split from the free label via a wash step or column. (news-medical.net)
  • IgA3.0 ch14.18 was evaluated in binding assays and in ADCC and antibody-dependent cellular phagocytosis (ADCP) assays with human, neuroblastoma patient and non-human primate effector cells. (bmj.com)
  • In in vitro phagocytosis assays, all fish isolates of GBS activated a respiratory burst in P2 indicated by significant production of intracellular reactive oxygen species (ROS). (qld.gov.au)
  • Consequently, the new algorithm is able to segment and characterize unlabeled cells, thus reducing the time and expense that would be spent on the fluorescent labeling in preparation for phagocytosis assays. (hki-jena.de)
  • The Fc receptor-dependent function of antibody-dependent cellular phagocytosis (ADCP) provides mechanisms for clearance of virus and virus-infected cells, as well as for stimulation of downstream adaptive immune responses by facilitating antigen presentation, or by stimulating the secretion of inflammatory mediators. (frontiersin.org)
  • Disrupting the binding of CD47 to SIRPα has emerged as a promising immunotherapeutic strategy for advanced cancers by potentiating antibody-dependent cellular phagocytosis (ADCP) of targeted antibodies. (aacrjournals.org)
  • Antibody-dependent phagocytosis (ADP) is a potentially important immune mechanism to clear HIV. (usuhs.edu)
  • The plasma membranes and intracellular membranes of live cells and the artificial membranes of liposomes represent a significant area of application for fluorescent probes. (thermofisher.com)
  • Data on phagocytosis in intracellular, biotrophic parasites are scant. (uhi.ac.uk)
  • Phagocytosis, where parts of the host cell are consumed at once, is seemingly at odds with intracellular biotrophy. (uhi.ac.uk)
  • We document intracellular phagocytosis in P. brassicae and M. ectocarpii by transmission electron microscopy and fluorescent in situ hybridization. (uhi.ac.uk)
  • Our investigations confirm molecular signatures of phagocytosis in Phytomyxea and hint at a small specialized subset of genes used for intracellular phagocytosis. (uhi.ac.uk)
  • Microscopic evidence confirms the existence of intracellular phagocytosis, which in Phytomyxea targets primarily host organelles. (uhi.ac.uk)
  • Phagocytosis, where parts of the host cell are consumed at once, is seemingly at odds with intracellular biotrophy.Here we provide evidence that phagotrophy is part of the nutritional strategy of Phytomyxea, using morphological and genetic data (including a novel transcriptome of M. ectocarpii). (uhi.ac.uk)
  • We document intracellular phagocytosis in P. brassicae and M. ectocarpii by transmission electron microscopy and fluorescent in situ hybridization.Our investigations confirm molecular signatures of phagocytosis in Phytomyxea and hint at a small specialized subset of genes used for intracellular phagocytosis. (uhi.ac.uk)
  • By using dose-response curve principles for both experimental design and analysis, it is possible to increase the sensitivity and robustness, leading to accurate quantification of phagocytosis that is comparable across experiments and systems.Here, we describe how to quantify phagocytosis using flow cytometry with a robust, high-throughput, and easy-to-use approach. (lu.se)
  • Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms. (ias-iss.org)
  • Moreover, the BAM-functionalised RPs were not eliminated via phagocytosis by macrophages-cells that play a key role in the immune system's defensive actions against bacterial invasions. (ecancer.org)
  • Furthermore, acidity\delicate phagocytosis by macrophages was looked into in tumor environment of TC\1 model in vivo. (oscars2019info.com)
  • Many feasible factors may donate to take into account this total result, such as for example (1) some receptors on macrophages could be particular bind by LDH@miR,42, 43 (2) moderate size of NPs was also added to endocytosis of macrophages,44 and 3) macrophage possess stronger phagocytosis capability than various other cells. (oscars2019info.com)
  • the ability of the macrophages to phagoytose indictor fluorescent beads and to migrate towards aC5a were determined. (hw.ac.uk)
  • the ability of the macrophages to phagoytose indictor fluorescent beads and to migrate towards aC5a were determined.Results: Ultrafine particles induced more PMN recruitment, epithelial damage, and cytotoxicity than their fine counterparts, exposed at equal mass. (hw.ac.uk)
  • Expression of sialic acids on virulent yeast forms of P. brasiliensis (3.7 X 10(6) residues per cell) may inhibit fungal phagocytosis during early infection, when the immunological response is still being built up. (unifesp.br)
  • Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions. (antibodiesinc.com)
  • Fluoresbrite® carboxylate microspheres are fluorescent monodispersed polystyrene microspheres that have carboxylate groups on their surfaces which can be activated for the covalent coupling of proteins. (parkscientific.com)
  • Further we studied phagocytosis of SWCNT by microglial cells. (cdc.gov)
  • Here, by immunohistochemical and immunofluorescence approaches, using the murine model of PHARC, we show, that upon an inflammatory stimulus, activated microglial cells in the cerebellum of mice deficient in ABHD12 have an amoeboid morphology, increased soma size, and display heightened phagocytosis activity. (fatcat.wiki)
  • Next, to complement these findings, using biochemical approaches in cultured microglial cells, we show that the pharmacological inhibition and/or genetic deletion of ABHD12 results in increased phagocytic uptake in a fluorescent bead uptake assay. (fatcat.wiki)
  • Together, our studies provide compelling evidence that ABHD12 plays an important role in regulating phagocytosis in cerebellar microglial cells, and provides a possible explanation, as to why human PHARC subjects display neuroinflammation and atrophy in the cerebellum. (fatcat.wiki)
  • Phagocytosis is involved in several functions including nutrient uptake, immune response, inflammation, tissue homeostasis, cellular apoptotic bodies and debris elimination. (allbiosolution.com)
  • Results support the hypothesis that surface of cell culture plates affects the cellular phagocytosis mechanism which play a critical role in the functions in many cases such as in vivo retina. (allbiosolution.com)
  • Phagocytosis is a complex trait of eukaryotes, well documented for free-living unicellular eukaryotes and specific cellular types of animals. (uhi.ac.uk)
  • The cellular response includes phagocytosis and encapsulation by hemocytes and pericardial cells. (vanderbilt.edu)
  • We continue to investigate mosquito cellular immune responses by using genetically engineered fluorescent bacteria to study hemocyte migration, adhesion, and pathogen killing efficacy in an organismal context, collecting data in vivo and in real-time, and visualizing the interaction between diverse physiological systems. (vanderbilt.edu)
  • Phagocytosis is an important evolutionary conserved process, essential for clearing pathogens and cellular debris in higher organisms, including humans. (fatcat.wiki)
  • The primary regulators of these immune responses are cells called hemocytes, which kill pathogens via phagocytosis and via the production of soluble antimicrobial factors. (biomedcentral.com)
  • In broad terms, mosquitoes kill pathogens via three mechanisms: cell-mediated phagocytosis, melanization, and lysis. (vanderbilt.edu)
  • Polysciences' Fluoresbrite® particles are used worldwide in phagocytosis and neural retrograde transport studies, and as markers for cell bound antigens. (parkscientific.com)
  • Aims: To compare the ability of ultrafine and fine particles of titanium dioxide and carbon black to induce inflammation, cause epithelial injury, and affect the alveolar macrophage clearance functions of phagocytosis and chemotaxis in vivo. (hw.ac.uk)
  • Fluorescent microscopy was applied and revealed that NBD-PS-SWCNT were a more preferred target of recognition and uptake by microlgial cells than NBD-PC-SWCNT. (cdc.gov)
  • Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. (wikipedia.org)
  • When acridine orange is excited by blue light, the fluorescent dye can differentially stain human cells green and prokaryotic cells orange (600 nm), allowing for rapid detection with a fluorescent microscope. (wikipedia.org)
  • Certain fluorescent fatty acids ( Fatty Acid Analogs and Phospholipids-Section 13.2 ) are readily metabolized by live cells to phospholipids, mono-, di- and triacylglycerols, cholesteryl esters and other lipid derivatives. (thermofisher.com)
  • In both the culicine and anopheline mosquito lineages there are several morphologically distinct classes of hemocytes: granulocytes are involved in the phagocytosis response, oenocytoids are involved in the melanization response, and prohemocytes are small cells of unknown function that have been hypothesized to serve as hematopoietic progenitors [ 14 - 16 ]. (biomedcentral.com)
  • tissue culture plate polystyrene and glass coverslip, on phagocytosis performed by RPE cells growing on each surface. (allbiosolution.com)
  • ARPE-19 cells were incubated for 4 and 24 hours with 0.2 and 1 μl red fluorescent latex beads, and then images were taken to measure the average numbers of cells, cells performing no phagocytosis and phagocytosis and engulfed red fluorescent latex beads. (allbiosolution.com)
  • There was no significant difference between numbers of ARPE-19 cells growing on polystyrene and glass surface performing latex bead phagocytosis. (allbiosolution.com)
  • Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture. (allbiosolution.com)
  • Likewise, Sertoli cells isolated from pre-pubertal rat testes have been marked for later detection by the phagocytosis of latex beads [ 10 ]. (biomedcentral.com)
  • Although the presence of cortactin in Sertoli cells has been addressed relative to its role in cell-cell adhesion [ 18 ], its role in phagocytosis has not yet been investigated. (biomedcentral.com)
  • Their surface location was inferred from fluorescent-lectin (Limulus polyphemus agglutinin) binding to whole cells abrogated by previous treatment with neuraminidase. (unifesp.br)
  • As the full total leads to Body ?Body2E,F,2E,F, LDH@miR demonstrated strong fluorescent indicators in Organic264.7 cells after incubation for 3h. (oscars2019info.com)
  • The cells in P2 had higher phagocytic index and capacity when incubated with fluorescent latex beads. (qld.gov.au)
  • However, unlike cold hemagglutinin disease, in which the IgM-complement interaction results in the cells' removal (via extravascular phagocytosis), paroxysmal cold hemoglobinuria occurs upon completion of complement lysis within the vascular circulation. (medscape.com)
  • Phagocytosis seems to coexist with the manipulation of host physiology typical of biotrophic interactions. (uhi.ac.uk)
  • Acridine orange is recommended for the use of fluorescent microscopic detection of microorganisms in smears prepared from clinical and non-clinical materials. (wikipedia.org)
  • Labelled beads were coated with both biotinylated HIV gp140 envelope protein and a fluorescent internalisation probe, opsonised with Abs and incubated with a monocytic cell line. (usuhs.edu)
  • The fluorescence derived from the fluorescent internalisation probe on surface-bound beads, but not from internalised beads, was quenched by the addition of a complementary quencher probe. (usuhs.edu)
  • Macrophage uptake of fluorescent liposome containing DiI. (encapsula.com)
  • As an example, we provide an experimental protocol for studying phagocytosis of opsonized Streptococcus pyogenes using the THP-1 cell line. (lu.se)
  • These data illustrated that whereas CD47/SIRPα inhibition could potentiate tumor cell phagocytosis, CD40-mediated activation of a type I interferon response provided a bridge between macrophage- and T-cell-mediated immunity that significantly enhanced durable tumor control and rejection. (aacrjournals.org)
  • The induced IgG and IgM antibodies were able to stimulate various Fc-mediated effector mechanisms associated with protection against malaria, including phagocytosis, release of reactive oxygen species, production of IFN-γ as well as complement activation and fixation. (nature.com)
  • abstract = "Phagocytosis is relevant for many research fields and is often measured as a functional outcome. (lu.se)
  • We also report that upon an inflammatory stimulus, cerebellar levels of ABHD12 increase to possibly metabolize the heightened oxidized PS levels, temper phagocytosis and in turn control neuroinflammation during oxidative stress. (fatcat.wiki)
  • Two immune functions are under study with same design: phagocytosis activity and oxidative burst. (hal.science)
  • NET) as a prognostic marker in respiratory isms, including phagocytosis, degranulation2, tract infectious diseases. (bvsalud.org)
  • When acridine orange associates with RNA, the fluorescent dye experiences a maximum excitation shift from 525 nm (green) to 460 nm (blue). (wikipedia.org)
  • and for investigating synaptosome recycling ( Probes for Following Receptor Binding and Phagocytosis-Section 16.1 ) and lipid-mediated signal transduction processes ( Probes for Signal Transduction-Chapter 17 ). (thermofisher.com)
  • Following this they aim to utilise the Lux 3's fluorescent settings to assess the impact of hypoxia on microglial processes including phagocytosis. (dwscientific.com)
  • Despite the biomedical significance of this lipase, detailed mechanistic studies and the specific contribution of ABHD12 to innate processes like phagocytosis remain poorly understood. (fatcat.wiki)
  • Incucyte ® FabFluor Antibody Labeling Reagents are Fc-region targeting Fab fragments that are conjugated to a pH-sensitive fluorescent probe. (news-medical.net)
  • THP1 are exposed to set of increasing concentration of toxic agent, cadmium and cell mortality are revealed using a fixable fluorescent probe. (hal.science)
  • Experimental factors like reaction volume, time, and phagocyte-prey concentrations often have a large impact on the outcome, as is the choice of detection strategy with different fluorescent or colorimetric labels of prey and phagocyte. (lu.se)
  • Based on this observation, we hypothesized that this actin binding protein may be associated with a non-junction-related physiological function, such as phagocytosis. (biomedcentral.com)
  • Next, we further looked into whether phagocytosis difference been around between weakened acid and regular physiological condition in vitro, in account of the weakened acid solution condition of tumor environment (pH 6.5). (oscars2019info.com)
  • There are five mannosylated fluorescent control liposome products (m-Fluoroliposome®) for m-Clodrosome® (mannosylated clodronate liposomes). (encapsula.com)
  • All five mannosylated fluorescent liposomes incorporate a lipophilic dye inside their membranes. (encapsula.com)
  • Membrane probes include fluorescent analogs of natural lipids, as well as lipophilic organic dyes that have little structural resemblance to natural biomolecules ( Figure 13.2.1 in Fatty Acid Analogs and Phospholipids-Section 13.2 ). (thermofisher.com)
  • Other, slightly less lipophilic probes are used as membrane markers of endocytosis and exocytosis ( Probes for Following Receptor Binding and Phagocytosis-Section 16.1 ). (thermofisher.com)
  • Our findings resolve long debated questions on the feeding behaviour of Phytomyxea, suggesting an unrecognized role for phagocytosis in biotrophic interactions. (uhi.ac.uk)
  • Next, the researchers attached a fluorescent "Alexa488-BSA" conjugate to the BAM-functionalised RPs, thus creating a bacterial complex with a trackable fluorescent marker. (ecancer.org)
  • These findings are very crucial when it comes to transfer a treatment approach based on phagocytosis to the clinics and that the materials and the conditions of the experiments can have very important consequences. (allbiosolution.com)
  • Bulk Order Inquiry for Anti-Green Fluorescent Protein Antibody ------- (please add any order requirements, including desired quantity, timing, etc. (antibodiesinc.com)
  • Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. (antibodiesinc.com)
  • Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund's adjuvant. (antibodiesinc.com)
  • Role of neutrophil extracellular la NETosis en las enfermedades infecciosas pulmonares. (bvsalud.org)
  • Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. (wikipedia.org)
  • Fluorescent phospholipid analogs ( Fatty Acid Analogs and Phospholipids-Section 13.2 ) can be classified according to where the fluorophore is attached. (thermofisher.com)
  • Our results show that maternal dietary n-3 PUFA deficiency increases microglia-mediated phagocytosis of synaptic elements in the rodent developing hippocampus, partly through the activation of 12/15-lipoxygenase (LOX)/12-HETE signaling, altering neuronal morphology and affecting cognitive performance of the offspring. (nature.com)
  • There are many ways to measure phagocytosis, but what is often overlooked is the importance of experimental design and how the analysis is planned and performed. (lu.se)