Fluorescent nucleotide. Medical search. Frequent questions

The primary amine on the aminoallyl nucleotide reacts with amino-reactive dyes such as a cyanine and patented dyes which contain a reactive leaving group, such as a succinimidyl ester (NHS).The amine groups directly attached to the ring of the base are not affected. (wikipedia.org)
After the enzymatic addition of the NTPs, amine reactant fluorescent dyes can be added for detection of the DNA molecule. (wikipedia.org)
Fluorescent dyes with aromatic amino or guanidine groups, such as propidium iodide (PI), diaminophenylindole (DAPI), acridine orange (AO), and Hoechst dyes, interact with nucleotides to emit fluorescence. (dojindo.com)
Cell membranes of viable cells are impermeable to these fluorescent dyes,except for the Hoechst dyes, and these dyes can therefore be used as fluorescent indicators of dead cells. (dojindo.com)
This has been enabled by introduction of chemically modified nucleotides with cleavable terminator groups and fluorescent dyes attached to them. (helsinki.fi)
Cy3 or Cy5-labeled), amine-labeled ddNTPs for subsequent coupling of NHS-ester-modified fluorescent dyes as well as biotin-labeled ddNTPs for subsequent detection with fluorescent streptavidin are available as catalog products. (jenabioscience.com)
This qualitative in-vitro diagnostics test uses oligonucleotide probes labeled with four different fluorescent dyes. (cdc.gov)
Thus, we could detect the odor substances through fluorescent quenching by using the fluorescence dyes. (fujipress.jp)
Fluorescence "in situ" hybridization (FISH) technique enables chromosomes to be tagged with fluorescent dyes to highlight genetic traits. (lu.se)

A cellular uptake assay with estrone-3-sulfate and a fluorescent probe 4', 5'-dibromofluorescein (DBF) as substrates was set up and optimized. (helsinki.fi)
In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)
The prevalence of single nucleotide polymorphisms in Plasmodium falciparum dihydropteroate synthase (pfdhps) and dihydrofolate reductase (pfdhfr) genes associated with SP resistance among 148 pregnant women from 2016 to 2018 within Rwanda's Southern Province (Huye and Kamonyi districts) was measured using a ligase detection reaction-fluorescent microsphere assay. (cdc.gov)

Dojindo offers a wide selection of fluorescent intracellular ion probes for calcium, proton, zinc, and chloride ions. (dojindo.com)
Dr. Tsien first developed fluorescent probes for calcium monitoring in viable cells. (dojindo.com)
Molecular beacons are hairpin shaped fluorescent oligonucleotide probes that can report the presence of a completely complimentary DNA target. (usda.gov)

Proteins were known to be composed of 20 distinct amino acids whereas DNA is composed of only 4 nucleotides. (proprofs.com)
In the early twentieth century, it was believed that proteins might carry genetic information because proteins were known to be composed of 20 distinct amino acids, whereas DNA is composed of only 4 nucleotides. (proprofs.com)
The most commonly stained cell components are cell membranes, proteins, and nucleotides. (dojindo.com)
The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
The effects of PAT on protein thiol levels were examined by reacting cellular proteins with the fluorescent thiol labeling probe monobromobimane. (cdc.gov)

However, it is now apparent that cAMP also exerts its effects by binding to cAMP-regulated guanine nucleotide exchange factors (Epac). (nih.gov)
The QPA reaction is based on formation of "an unusually stable monomolecular quadruplex" by a guanine rich sequence constructed with fluorescent nucleotides, Kankia said. (genomeweb.com)
Researchers sequence DNA to determine the order of the four chemical building blocks, or nucleotides, that make it up: adenine, thymine, cytosine and guanine. (indiatimes.com)
With the development of sequencing techniques, it is now possible to study the structure of the genome in detail, defined by the order of the nitrogenous bases (nucleotides) adenine, guanine, cytosine and thymine in the DNA. (lu.se)

The aim of this study was to identify any impact of CYP2A13 single-nucleotide polymorphisms (SNPs) on CYP2A13 expression in human lung. (aspetjournals.org)
To date, more than 20 single-nucleotide polymorphisms (SNPs) have been identified in CYP2A13 (Zhang et al. (aspetjournals.org)
Because of ARMS' ability to detect single nucleotide polymorphisms (SNPs) in heterozygote patients, the technique has been increasingly popular. (brighthub.com)
Single nucleotide polymorphisms (SNPs) are single base pair mutations at specific locations in the coding and non-coding regions of the genome that are found in more than 1% of a population [1] . (jenabioscience.com)
Single nucleotide polymorphisms (SNPs) are detected by enzymatic elongation of a primer by a SNP-complementary, fluorescently labeled ddNTP. (jenabioscience.com)
Single nucleotide polymorphisms (SNPs) were identified that are closely associated with rhg1 and rhg4, two important loci controlling resistance to SCN. (usda.gov)

Effects of antimony on mitochondrial function and protein thiol and adenine nucleotide status in cultured cardiac myocytes. (cdc.gov)
The effects of PAT on cellular ATP levels were assessed by measuring total adenine nucleotides by HPLC with uv detection. (cdc.gov)

designated ATPOS) shows a large fluorescence change in the presence of ATP, with submicromolar affinity, pH-independence, and high selectivity for ATP over ATP metabolites and other nucleotides. (elifesciences.org)
The reaction is also monitored by intrinsic fluorescence of the primer, which is constructed with fluorescent nucleic acids, whereas all other isothermal methods use external detection methods. (genomeweb.com)
The 5' end of a molecular beacon contains a fluorescent dye and the 3' end a fluorescence-quenching moiety. (usda.gov)

These nucleotides are known as 5-(3-aminoallyl)-nucleotides since the aminoallyl group is usually attached to carbon 5 of the pyrimidine ring of uracil or cytosine. (wikipedia.org)

Select a suitable nucleotide substrate for enzymatic DNA Labeling. (jenabioscience.com)
Table 1: Enzymatic incorporation of fluorescent Nucleotides. (jenabioscience.com)

Although direct labeling of dNTP is the quickest and cheapest method of fluorescent labeling, it is disadvantageous as the sequence allows for only one modified nucleotide for use. (wikipedia.org)
A confocal scanner then reads the fluorescent intensity of each hybridized sequence in the array. (medscape.com)
the mutant primer is matched at the mutant nucleotide and mismatched at the normal sequence. (brighthub.com)
For example, the Illumina NovaSeq can sequence roughly 150 billion nucleotides, the equivalent of 48 human genomes, in just three days. (indiatimes.com)
Nucleotide sequence analysis of viral RNA implicated a variant associated with silver-haired (Lasionycteris noctivagans) and eastern pipistrelle (Pipistrellus sp. (cdc.gov)

Figure 4: Sequencing by synthesis: single-nucleotide addition approaches. (nature.com)
A mismatched 3' end of a the primer prevents nucleotide expansion during DNA synthesis in a PCR reaction, and, consequently, no reaction occurs. (brighthub.com)

The 450 C-terminal nucleotides of the N gene in MV contains enough nucleotide variations to allow discrimination among genotypes. (brighthub.com)
yunnanensis should be divided into two types of genotypes: YN-I and YN-II according to single nucleotide polymorphism of internal transcribed spacer. (researchsquare.com)

Nucleotide staining with fluorescent intercalators is mostly used for dead cell detection. (dojindo.com)
Subsequent detection of the incorporated ddNTP is performed by fluorescent visualization of the extended primer by electrophoresis or array-based methods, thus revealing the nucleotide base at that position on the template strand. (jenabioscience.com)
To avoid pH dependence of the fluorescent signal in calcium detection, Dr. Tsien changed the aliphatic amines of EGTA to aromatic amines, thereby transforming EGTA into a new calcium chelator, BAPTA. (dojindo.com)

made several potential sensors by attaching various fluorescent tags to different locations on a protein that binds ATP. (elifesciences.org)
The order of these 4 nucleotides makes up the genetic code and provides the instructions to make every protein within an organism. (addgene.org)
This protein is known to act as an important sensor in the Global Genome Nucleotide Excision Repair (GG-NER) by recognizing sites of UV-induced DNA lesions [ 2 ]. (biomedcentral.com)

the method of aminoallyl nucleotide incorporation was opted instead. (wikipedia.org)

This method relies on the extension of a primer , designed to bind one nucleotide upstream of the polymorphic spot, by a SNP-complementary, fluorescently labeled ddNTP [5] . (jenabioscience.com)

[ 7 ] They consist of a glass surface onto which oligonucleotides consisting of 25 bases are built a single nucleotide at a time. (medscape.com)
Furthermore, the aim was to study the effects of six naturally occurring nonsynonymous single nucleotide variants on OATP2B1 transport function in vitro. (helsinki.fi)
With site-directed mutagenesis, single nucleotide changes were introduced into the gene coding for OATP2B1. (helsinki.fi)
EcoRI, NotI, and BamHI from multiple suppliers were tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3'overhang or 5' overhang containing oligonucleotides. (neb.com)
The resolution is at the single nucleotide level. (neb.com)
1999) From gels to chips: Minisequencing primer extension for analysis of point mutations and single nucleotide polymorphisms. (jenabioscience.com)
2011) Clinical relevance of multiple single-nucleotide polymorphisms in Pneumocystis jirovecii Pneumonia: development of a multiplex PCR-single-base-extension methodology. (jenabioscience.com)
Single base extension and cytometric analysis of fluorescent microspheres was used to confirm the results of these two techniques. (usda.gov)
There is strong evidence that inherited genetic factors (mutations plus single nucleotide polymorphisms) can play a major role in breast cancer susceptibility [ 1 ]. (hindawi.com)
This process helps identify single or multiple nucleotide variations as well as areas of insertion or deletion. (msdmanuals.com)
2017 to March 2018, there was circulation of both detect a single target in a very small concentration of DNA influenza A and B, with influenza A(H1N1)pdm09 again or RNA because it uses a fluorescent dye that binds to predominating in Viet Nam. (who.int)

After DNA is cut up into fragments, short stretches of genetic material called adapters are added to give each nucleotide a different color. (indiatimes.com)

Their fluorescent signal intensities or wavelengths change because each intracellular calcium probe has a calcium-selective chelator and a fluorescent moiety in its molecule. (dojindo.com)
To prepare a calcium probe, which shifts its fluorescent wavelength and changes its fluorescent intensity upon calcium chelation, a suitable fluorescent part must be selected. (dojindo.com)
The rs3213172, rs3213173, and rs3213176 polymorphisms of the E2F1 gene were detected by the fluorescent probe method. (hindawi.com)

Red fluorescent genetically encoded calcium indicators (GECIs) have expanded the available pallet of colors used for the visualization of neuronal calcium activity in vivo. (mdpi.com)
Visualization of a cell with fluorescent compounds provides a wide variety of information for the analysis of cell functions. (dojindo.com)
Subsequent fluorescent visualization of the product reveals the incorporated nucleotide and hence the SNP genotype. (jenabioscience.com)

In this specific case, the aaUTP modified RNAs were tagged with fluorescent market Cy3. (wikipedia.org)

The plateau-free method also happens to be isothermal, and is intrinsically fluorescent, making it suitable for use in point-of-care diagnostic assays - an application the OSU group is now working with unnamed entities to develop. (genomeweb.com)

The library is loaded into a sequencer, which will identify the nucleotide bases in the DNA fragments. (cdc.gov)
One of the earliest methods scientists used in the 1970s and 1980s was Sanger sequencing, which involves cutting up DNA into short fragments and adding radioactive or fluorescent tags to identify each nucleotide. (indiatimes.com)

Whole-genome sequencing (WGS) is a laboratory procedure that determines the order of all or most of the nucleotides in the genome of these disease-causing microbes, enabling public health officials to better understand how microorganisms move through populations and change over time. (cdc.gov)

Because each pair of nucleotides disrupts the electrical current in a particular way, the sequencer can read these changes and upload them directly to a computer. (indiatimes.com)

If the calcium ion binds to indicators, donation of the lone electron pair on the aromatic nitrogen to the calcium ion causes a change in the fluorescent signaling. (dojindo.com)

Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. (utmb.edu)

Cybulski TR, Boyden ES, Church GM, Tyo KEJ, Kording KP (2017) Nucleotide-time alignment for molecular recorders, PLoS Computational Biology 13(5):e1005483. (mit.edu)

After each base is incorporated by a polymerase and imaged, its fluorescent tag is removed and another base can be added. (acs.org)

These sequences are composed of chemical building blocks known as nucleotide bases. (cdc.gov)

Fluorescent esterase substrates may also be used in cell viability assays in place of tetrazolium analogs such as MTT or WST. (dojindo.com)

This means that a deprotonation step is involved in the calcium binding process, which causes a change in the fluorescent signal. (dojindo.com)
Thus, this type of calcium indicator is practically non-fluorescent. (dojindo.com)
Once the calcium complex is formed, the contribution of the lone electron pair disappears and the original xanthene fluorescent signal is markedly recovered (as shown in Fig. 1). (dojindo.com)

They are reactive with N-Hydroxysuccinimide ester group which helps attach a fluorescent dye to the primary amino group on the nucleotide. (wikipedia.org)
Each amino acid is encoded for by 3 nucleotides termed a codon. (addgene.org)

This research along with others, which included various types of direct and non-direct labeling via: analogs, addition via enzymes, or other methods made labeling of nucleotides much safer for scientist to study DNA. (wikipedia.org)

By the joining one or more phosphate groups to a nucleoside through ester linkages, a nucleotide is formed. (wikibooks.org)
Succinimidyl ester compounds can also be used to improve retention of the fluorescent derivative within the cell. (dojindo.com)

Fluorescent staining using CTC is another method used to detect viable bacterial cells. (dojindo.com)

The nucleotide includes the nucleoside, a nitrogenous base bonded to a deoxyribose or ribose group. (wikibooks.org)

The first base modification for fluorescent labeling occurred in 1971 with a 4-thiouridine and 4-thiouracil. (wikipedia.org)
But so far this year, the group has published five studies on QPA, describing its modification with a new fluorescent nucleotide and compatibility with a range of temperatures and polymerases . (genomeweb.com)

The deoxyribose sugars form the structural backbone for DNA via a phosphodiester bond between the 3' carbon of one nucleotide and the 5' carbon of the next. (wikibooks.org)

Positive samples were inoculated in MDCK cells and virus phenotypic susceptibility to neuraminidase inhibitors (NAIs) was assessed using fluorescent NA inhibition. (who.int)

An avidity reagent with a fluorescent tag (green) includes multiple moieties of the same nucleotide, which can match multiple copies of the same template strand (inset box), increasing overall binding strength. (acs.org)

Now, companies are introducing sequencing platforms that separate fluorescent labeling from the extension of the complementary DNA strand, touting improvements in accuracy that result from optimizing each step. (acs.org)

Once converted into fluorescent products by esterase, these compounds are retained by cells because of their negative charges. (dojindo.com)

In the study, researches took 30 isolates from MV outbreaks and analyzed the samples with a method of RT-ARMS PCR along with SYBR green fluorescent dye. (brighthub.com)

In order to avoid false-negative results, two pairs of specific primers for YN-I and YN-II were designed, respectively, and specific visual fluorescent identification systems was established by SYBR Green I fluorescent dye was directly introduced into the PCR system which can be observed directly with the naked eye for the green fluorescent color of PCR system. (researchsquare.com)

However, on October 18, rabies was diagnosed by the direct fluorescent antibody (DFA) test from postmortem brain samples tested by the Houston Department of Health and Human Services. (cdc.gov)

Like individual Velcro hooks, each nucleotide can base pair with one identical copy of an amplified template, increasing the overall strength of the interaction. (acs.org)

Anatomy1

Organisms1

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes34

Information Science4

Dyes9

Assay3

Probes3

Proteins5

Guanine4

SNPs6

Adenine2

Fluorescence3

Cytosine1

Enzymatic2

Sequence5

Synthesis2

Genotypes2

Detection3

Protein3

Incorporation1

Polymorphic1

Single11

Genetic1

Probe3

Visualization3

RNAs1

Suitable1

Fragments2

Genome1

Sequencer1

Indicators1

Terminator1

Molecular1

Polymerase1

Bases1

Substrates1

Calcium3

Amino2

Enzymes1

Ester2

Detect1

Nitrogenous1

Modification2

Structural1

Susceptibility1

Template strand1

Complementary1

Products1

Method1

Color1

Human1

Base pair1