Fluorescence species proteins genes bacterial. Medical search. Frequent questions

To do so, we mostly rely on fluorescence microscopy techniques such as confocal laser scanning microscopy and widefield epifluorescence microscopy. (canterbury.ac.nz)

Using this new technique, we can cut a strawberry's genome at a desired location so that existing genes can be removed or added. (ufl.edu)
Using de novo gene prediction, we identified 6,996 protein-encoding genes in the MAST-4 genome. (nature.com)
Analysis of the genome data using ab initio gene prediction identified 6,996 protein-encoding genes in the genome of the isolate. (nature.com)
E. coli is our desired "skeleton" bacteria for our project due to the bacteria's genome already containing many key proteins in the desired pathway. (igem.org)
Comparative genome analysis suggests genes that play a role in the construction or functioning of large cable bacteria cells: the genomes of Ca . Electrothrix gigas encode a novel actin-like protein as well as a species-specific gene cluster encoding four putative pilin proteins and a putative type II secretion platform protein, which are not present in other cable bacteria. (uibk.ac.at)
The search for specific gene fragments from the target bacterial genome, and the development of a corresponding detection method, could be the key factor to solve this fundamental problem. (biomedcentral.com)
Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. (sigmaaldrich.com)
1 Covering over 17,000 human and over 20,000 mouse genes with two optimized gRNAs for each of them, our Sanger Whole Genome Arrayed Libraries are powerful tools that can help you make the next exciting discovery. (sigmaaldrich.com)
Our exclusive Sanger CRISPR library provides extensive, high-quality gene knockout, so you can fast-track your research with the first whole genome arrayed library. (sigmaaldrich.com)
The effect of Buchnera genome evolution on gene expression levels has also been analysed in order to assess the constraints imposed by the obligate symbiosis with aphids, underlining the importance of some gene sets for the survival of the two partners. (biomedcentral.com)
The degree of organization has been shown to increase with genome size, overall GC composition and the presence of nucleoid-binding proteins [ 1 ]. (biomedcentral.com)
2006. Assessing the potential of regions of the nuclear and mitochondrial genome to develop a 'molecular tool box' for the detection and characterization of Phytophthora species . (hutton.ac.uk)
Expanding duplication of the testis PHD Finger Protein 7 (PHF7) gene in the chicken genome. (inra.fr)
We aimed to explore and characterise in the chicken genome the expanding family of the numerous orthologues of the unique mouse Phf7 gene (highly expressed in the testis), observing the fact that this information is unclear and/or variable according to the versions of databases. (inra.fr)
We showed that the PHF7 gene, which is highly expressed in the rooster testis, is a highly duplicated gene family in the chicken genome, and this phenomenon probably concerns other bird species. (inra.fr)
In the 1980s, Mario Capecchi 1st founded gene-targeting CAB39L technology, along with the concept of harnessing double-strand breaks (DSBs) for genome editing (Capecchi, 1980). (baxkyardgardener.com)
Therefore, in this study, to select P. odoriferum -specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. (ppjonline.org)
aquaculture production has experienced a great increase in the last decade and, consequently, the genome knowledge of the species is gaining attention. (metasystems-international.com)
In this sense, obtaining a high-density genome mapping of the species could offer clues to the aquaculture improvement in those aspects not resolved so far. (metasystems-international.com)
A total of 93 BAC clones were used to localize the chromosome complement of the species and 588 genes were annotated, thus almost reaching the 2.5% of the genome sequences. (metasystems-international.com)
As a result, important data about its genome organization and evolution were obtained, such as the lesser gene density of the large metacentric pair compared with the other metacentric chromosomes, which supports the theory of a sex proto-chromosome pair. (metasystems-international.com)
In its simplest form, it involves the introduction of a non-mutated gene at a random location in the genome to cure a disease by replacing a protein that may be absent in these individuals because of a genetic mutation . (opentextbc.ca)
The non-mutated gene is usually introduced into diseased cells as part of a vector transmitted by a virus, such as an adenovirus, that can infect the host cell and deliver the foreign DNA into the genome of the targeted cell ( Figure 10.8 ). (opentextbc.ca)

However, using fluorescence and confocal microscopy (which are imaging techniques), the Cambridge team has dispelled this dogma concerning the requirement for the SPI-2 T3SS for intracellular replication in the body. (phys.org)
10 6 CFU/ml) and S. aureus to (10 7 CFU/ml) were concurrently added to 96-well tissue culture-treated chamber slides and incubated for 24 h at 37°C. Biofilm formation was monitored by fluorescence microscopy and scanning electron microscopy. (asmblog.org)
The team used an advanced imaging technology called "zero-mode waveguide single-molecule fluorescence microscopy," which they have adapted in recent years for real-time tracking of RNAs and proteins. (scripps.edu)
After 18 h cells were fixed with phosphate-buffered saline (PBS) plus 10% formalin and MNGCs were observed by fluorescence microscopy. (healthandwellnesssource.org)
The team led by Gerhard Hummer, Director at the Max Planck Institute of Biophysics, and Edward Lemke, Professor for Synthetic Biophysics at Johannes Gutenberg University Mainz, and Adjunct Director at the Institute of Molecular Biology Mainz has now used a novel combination of synthetic biology, multidimensional fluorescence microscopy and computer-based simulations to study nuclear pore IDPs in living cells. (mpg.de)
Use of this method to target doublesex (a gene involved in sex differentiation) or fork head (which encodes a salivary gland transcription factor) resulted in reduced target gene expression and/or protein immunofluorescence signal, as measured by quantitative Real-Time PCR (qRT-PCR) or fluorescence confocal microscopy, respectively. (cdc.gov)
We developed an optimized method for larval SG staining and surveyed hundreds of larval stage 4 (L4) SGs using fluorescence confocal microscopy. (cdc.gov)

Cobalamin riboswitch is a cis-regulatory element which is widely distributed in 5' untranslated regions of vitamin B12 (Cobalamin) related genes in bacteria. (wikipedia.org)
As of 2019, cobalamin riboswitches have been identified in over 5000 species of bacteria. (wikipedia.org)
Bioinformatic studies were initially unsuccessful in identifying AdoCbl riboswitches within the bacteria genomes, but subsequent studies of the intergenic regions of the eutG locus using Ribex identified an RNA element between the eutT and eutG genes. (wikipedia.org)
This in combination with bacteria that are either tagged with fluorescent protein genes, stained using fluorescence in situ hybridisation (FISH), or stained using fluorescent live/dead stains that allow to determine if individual bacterial cells are either alive or dead. (canterbury.ac.nz)
In contrast to the free-living, or planktonic, bacteria commonly studied in the lab, most prokaryotes in nature settle down in complex communities, often consisting of several interacting species. (the-scientist.com)
Biofilms form when groups of bacteria cover themselves in a sticky mixture of sugars, protein, and DNA. (the-scientist.com)
Bacteria sense these microenvironments and adapt by up or down regulating the expression of certain genes to influence different cell processes. (the-scientist.com)
The laboratory of Vladislav Verkhusha, Ph.D., associate professor of anatomy and structural biology at Einstein and the study's senior author, engineered a fluorescent protein from a bacterial phytochrome, the pigment that a species of bacteria uses to detect light. (laserfocusworld.com)
This ability has not been lost on human bioengineers who remove the DNA that the bacteria wants to deliver and replaces it with the gene being studied. (rosslandtelegraph.com)
It targets gram-positive and gram-negative bacteria and several Mycoplasma species. (goldbio.com)
The team also presented a new role for the NADPH phagocyte oxidase (Phox) (a host mechanism which generates reactive oxygen species which can inhibit the growth and/or kill the bacteria) in the control of Salmonella infection. (phys.org)
Bacteria are typically found as communities made up of several different species rather than as isolated, solitary organisms in nature ( 6 ). (frontiersin.org)
Fluorescence in situ hybridization with a species-level probe showed that large-sized cable bacteria belong to a novel species with the proposed name Ca . Electrothrix gigas. (uibk.ac.at)
The novel actin-like protein was also found in a number of other giant bacteria, suggesting there could be a genetic basis for large cell size. (uibk.ac.at)
This actin-like protein (denoted big bacteria protein, Bbp) may have a function analogous to other actin proteins in cell structure or intracellular transport. (uibk.ac.at)
IMPORTANCE In this study, we substantially expand the known diversity of marine cable bacteria and describe cable bacteria with a large diameter as a novel species with the proposed name Candidatus Electrothrix gigas. (uibk.ac.at)
The bbp gene was also found in a number of other giant bacteria, predominantly affiliated to Desulfobacterota and Gammaproteobacteria, indicating that there may be a genetic basis for large cell size. (uibk.ac.at)
Light blue cells represent bacteria with an active promoter, grey cells mark the total bacterial mass. (siamtech.net)
In order to develop a rapid and accurate method of bacterial identification, we studied the possibility of identifying bacteria with other characteristic fragments without the use of 16S rRNA as detection targets. (biomedcentral.com)
We analyzed the potential of using cas (CRISPR-associated proteins) gene as a target for bacteria detection. (biomedcentral.com)
Consequently, at the species level only a few bacteria can be identified using the technology, with an inability to distinguish intestinal flora among sub-species or strains. (biomedcentral.com)
Genomic studies on bacteria have clearly shown the existence of chromosomal organization as regards, for example, to gene localization, order and orientation. (biomedcentral.com)
Moreover, transcriptomic analyses have demonstrated that, in free-living bacteria, gene transcription levels and chromosomal organization are mutually influenced. (biomedcentral.com)
This is a perfect illustration of the mutual influence between gene expression levels and chromosomal organization in bacteria. (biomedcentral.com)
The occurrence of RIP genes has been described in a wide range of plant taxa, as well as in several species of bacteria and fungi. (cdc.gov)
In bacteria, the function of the Rabbit Polyclonal to SAA4 tmRNA molecules is to release BMS-354825 cost ribosomes that have become stalled during protein synthesis and to tag incomplete and unnecessary peptides for proteolysis. (baxkyardgardener.com)
Modern techniques use specific genes of microorganisms cloned into vectors and mass-produced in bacteria to make large quantities of specific substances to stimulate the immune system. (opentextbc.ca)
Although several recombinant proteins used in medicine are successfully produced in bacteria, some proteins need a eukaryotic animal host for proper processing. (opentextbc.ca)

He used a tool known as parallel sequential fluorescence in situ hybridization (par-seqFISH) on populations of Pseudomonas aeruginosa, a species often found in the lungs of people with cystic fibrosis. (the-scientist.com)
With that in mind, he and his colleagues turned to gene expression profiling, single-cell RNA sequencing, and fluorescence in situ hybridization (FISH) to assess uterus endometrial fibroblast and ovarian endometriotic lesion samples from individuals with endometriosis and samples from unaffected controls. (genomeweb.com)

The study by the Rutgers/UCLA team used single-molecule fluorescence spectroscopy. (phys.org)

Based on 16S rRNA gene studies, the group PAUC43f is one of the most frequently retrieved Gemmatimonadota in marine samples. (uibk.ac.at)
The traditional method of bacterial identification based on 16S rRNA is a widely used and very effective detection method, but this method still has some deficiencies, especially in the identification of closely related strains. (biomedcentral.com)
The casx gene-based method of bacterial identification could be used as a supplement to the conventional 16 s rRNA-based detection method. (biomedcentral.com)
Phylogenetic affiliations of mesopelagic acantharia and acantharian-like environmental 18S rRNA genes off the southern California coast. (mbl.edu)
Ribosome Inactivating Proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of the 28S rRNA. (cdc.gov)

This is a false colored mesoscopic image of two strains of E. coli expressing different fluorescent proteins. (the-scientist.com)
An expansion of the six current nomen species of Brucella has been proposed to include one ( B. maris ) or two ( B. pinnipediae and B. cetaceae ) new nomen species to categorize these strains ( 7 , 8 ). (cdc.gov)
For all major bacterial pathogens, including tuberculosis, strains such as XDR-TB that are resistant to current antibiotics have emerged. (phys.org)
MATERIALS AND METHODS Bacterial strains and mutant building. (healthandwellnesssource.org)
In some cases, such as the H1N1 flu vaccine, genes cloned from the virus have been used to combat the constantly changing strains of this virus. (opentextbc.ca)

Ligand binding mediates allosteric rearrangement of mRNA structure, and this results in modulation of gene expression or translation of mRNA to yield a protein. (wikipedia.org)
Ligand binding induces an allosteric structural rearrangement in the expression platform that results in the regulation of gene expression via transcriptional or translational mechanisms. (wikipedia.org)
The expression of the ethanolamine utilization genes (eutG) is influenced by two different mechanisms. (wikipedia.org)
Clearly, interspecies bacterial communication influences the regulation of gene expression. (asmblog.org)
Fusing the AOXI gene promoter with the DNA sequence encoding a fluorescent protein will allow the expression of the AOXI gene to be detected. (igem.org)
GFP emits green fluorescent light when exposed to blue or ultraviolet light, and the degree of fluorescence can be used to quantify the gene expression of whatever DNA sequence to which the gfp -reporter gene is fused. (uark.edu)
They use Hi-C and related techniques to study the control of expression of clustered biosynthetic pathway genes in Arabidopsis. (garnetcommunity.org.uk)
Gene organization in the bacterial chromosome has very well known effects on transcription and a standard example is the organization of genes into operons, which allows for a sophisticated regulation of gene expression [ 9 ]. (biomedcentral.com)
2006. An atlas of gene expression from seed to seed through barley development . (hutton.ac.uk)
We sought to better understand larval SG architecture, secretion, and gene expression. (cdc.gov)
Meta-analysis of microarray data showed that larval SG gene expression is divergent from adult SGs, more similar to larval gastric cecae, but different from other larval gut compartments. (cdc.gov)

This T3SS is encoded by genes in a region of the bacterial chromosome called Salmonella Pathogenicity Island 2 (or SPI-2). (phys.org)
Nützmann HW, Doerr D, Ramírez-Colmenero A, et al (2020) Active and repressed biosynthetic gene clusters have spatially distinct chromosome states Proc Natl Acad Sci U S A. 2020;201920474. (garnetcommunity.org.uk)
This study reveals potential mechanisms that suggest gene clustering in the one-dimensional chromosome is accompanied by compartmentalization of the 3D chromosome. (garnetcommunity.org.uk)
Past genomic studies have comprehensively described the organization of the bacterial chromosome, for example in terms of gene localization, order and orientation. (biomedcentral.com)
This organization of the chromosome can be described as being an adaptive and functional tool, essential for the survival of the bacterial cell. (biomedcentral.com)
It has also been shown that neighbouring genes in a bacterial chromosome tend to be co-expressed, even if they are not in the same operon [ 10 ], suggesting the existence of a "supra-operonic" organization [ 11 ]. (biomedcentral.com)
We validated nine primer pairs by in silico PCR for their use in screening the chicken bacterial artificial chromosome (BAC) library to produce BAC-derived probes to detect and localise PHF7-like loci by fluorescence in situ hybridisation (FISH). (inra.fr)
This kind of result widens the knowledge of this species' chromosome dynamics and evolution. (metasystems-international.com)
We measured the meiotic recombination rate in two regions of chromosome 5, containing or not an NLR gene cluster. (metasystems-international.com)

A more detailed analyses on bacterial-fungal co-cultivation in narrow interaction channels of microfluidic devices revealed that the strongest inhibitory potential was found for Pseudomonas protegens CHA0, with its inhibitory potential depending on the presence of the GacS/GacA system controlling several bacterial metabolites. (frontiersin.org)
As the control center of the cell, the nucleus must be able to exchange important messenger molecules, metabolites or proteins with the rest of the cell. (mpg.de)

The fourth paper is from Cambridge and looks at the role of the ASY1 protein during meiotic recombination. (garnetcommunity.org.uk)
Deciphering the Impact of a Bacterial Infection on Meiotic Recombination in Arabidopsis with Fluorescence Tagged Lines. (metasystems-international.com)

This interplay of bacterial effects on the pathogen can be beneficial to protect plants from infection, as shown with A . thaliana root experiments. (frontiersin.org)
One major disease resistance mechanism is based on NB-LRR (NLR) proteins that specifically recognize pathogen effectors. (metasystems-international.com)

They used FISH probes informed by publicly available bacterial 16S ribosomal RNA databases to search for Fusobacterium species found in some vaginal samples in past studies. (genomeweb.com)
This island contains ribosomal proteins or RNA related elements and may indicate a False Positive Prediction! (up.ac.za)
The achievement, reported in Cell , reveals in unprecedented detail how strands of ribonucleic acid (RNA), cellular molecules that are inherently sticky and prone to misfold, are "chaperoned" by ribosomal proteins into folding properly and forming one of the main components of ribosomes. (scripps.edu)
This involved the transcription, or copying out from its corresponding gene, of a ribosomal RNA, and initial interactions of this RNA strand with a ribosomal protein. (scripps.edu)
A key finding was that the ribosomal protein partners guide the folding of the RNA strand through multiple temporary interactions with the strand, well before they nestle into their final places in the folded RNA-protein molecule. (scripps.edu)
Our study indicates that in ribosomal RNA-folding, and perhaps more generally in RNA-folding in cells, many proteins help fold RNA though weak, transient and semi-specific interactions with it," Duss says. (scripps.edu)

The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)
C) Detection of eDNA content in biofilms by Ethidium Homodimer III staining and fluorescence intensity measurements at 535/595 nm. (siamtech.net)

Translating this cell culture work into whole animals, it has become accepted dogma that the SPI-2 T3SS is also required for bacterial intracellular replication in cells inside the body. (phys.org)
In analogy to other actin proteins, Bbp could fulfill a structural role in the cell or potentially facilitate intracellular transport. (uibk.ac.at)
Pseudomallei group species are facultative intracellular parasites that spread efficiently from cell to cell by a mechanism involving the fusion of adjacent cell membranes. (healthandwellnesssource.org)

Fluorescence of the liver in the infected mice was first detected the second day after infection and reached a peak at day five. (laserfocusworld.com)
With the help of follow-up immunohistochemistry, chromatin immunoprecipitation-qPCR, cytokine profiling, mouse modeling, and other experiments, the team suggested that Fusobacterium infection can boost transforming growth factor beta (TGF-beta) activation, prompting quiescent fibroblast cells to become myofibroblast cells expressing a cytoplasmic microfilament-associated protein known as transgelin (TAGLN). (genomeweb.com)

Here, we investigated the effect of pretreatment with exogenous melatonin on the net photosynthetic rate, the maximum photochemical efficiency of PSII, chlorophyll content, lipid peroxidation, reactive oxygen species (ROS) accumulation, antioxidant potential, and redox homeostasis in leaves of tea plants following cold stress. (mdpi.com)

For the past 20 years, scientists have used a variety of colored fluorescent proteins, derived from jellyfish and corals, to visualize cells and their organelles and molecules. (sciencedaily.com)
This area has given rise to organisms such as fish that glow in the dark due to the addition of jellyfish genes that cause fluorescence. (rosslandtelegraph.com)

Velanis CN, Perera P, Thomson B, et al (2020) The domesticated transposase ALP2 mediates formation of a novel Polycomb protein complex by direct interaction with MSI1, a core subunit of Polycomb Repressive Complex 2 (PRC2) PLoS Genet. (garnetcommunity.org.uk)

In addition, bacterial pathogens that may be used in bioterrorism can be engineered, and, in the former Soviet Union, were intentionally engineered to be resistant to current antibiotics. (phys.org)
The scientists found out how intrinsically disordered proteins in the center of the pore can form a spaghetti-like mobile barrier that is permeable for important cellular factors but blocks viruses or other pathogens. (mpg.de)
There also is a need to monitor for potential adverse consequences of intrapartum antibiotic prophylaxis (e.g., emergence of bacterial antimicrobial resistance or increased incidence or severity of non-GBS neonatal pathogens). (cdc.gov)

The next paper introduces genes from the parasitic plant Striga hermonthica into Arabidopsis to show that strigolactone signaling can replace GA signaling in the control of seed germination. (garnetcommunity.org.uk)
Christos Velanis is first author on this research led by the Goodrich group at the University of Edinburgh that looked at the function of the Arabidopsis ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 (ALP1) gene, which has arose by domestication of the Harbinger class of transposable elements (TEs). (garnetcommunity.org.uk)

Bacterial, fungal, and acid-fast bacilli stains were negative. (cdc.gov)
Thus, the secondary metabolite mediated effect of Pseudomonas isolates on Verticillium species results in a complex transcriptional response, leading to decreased growth with precautions for self-protection combined with the initiation of a change in fungal growth direction. (frontiersin.org)

We analyzed the genomes of cable bacterium filaments from 11 coastal environments of which the resulting 23 new genomes represent 10 novel species-level clades of Candidatus Electrothrix and two clades that putatively represent novel genus-level diversity. (uibk.ac.at)
To help you unravel your genes and pathways of interest, we collaborated with The Wellcome Sanger Institute to make the first arrayed lentiviral CRISPR knockout libraries for human and mouse genomes. (sigmaaldrich.com)
Indeed, bacterial genomes carry, on average, from 78% (for genomes containing the polymerase PolC) to 58% (for the other genomes) of their genes on the leading strand [ 3 ]. (biomedcentral.com)

This highly flexible, user-friendly, low-cost, time-efficient method of dsRNA delivery could be broadly applicable to target genes important for insect vector physiology and beyond. (cdc.gov)
Figure 10.8 This diagram shows the steps involved in curing disease with gene therapy using an adenovirus vector. (opentextbc.ca)
The HGH gene was cloned from a cDNA (complementary DNA) library and inserted into E. coli cells by cloning it into a bacterial vector. (opentextbc.ca)

Comprehensive set of (differentially portrayed) genes. (siamtech.net)

the new research opens up the possibility of designing future antibiotics that target bacterial ribosomes with greater specificity-and thus, fewer side effects. (scripps.edu)

2021. On the systematics and the phylogenetic position of the poorly known, montane dragon-lizard species Pseudocalotes austeniana (Annandale, 1908) (Squamata, Agamidae, Draconinae) . (ncbs.res.in)

Our analysis showed that mRNA abundances, gene organization (operon) and gene essentiality are correlated in Buchnera (i.e., the most expressed genes are essential genes organized in operons) whereas no link between mRNA abundances and gene strand bias was found. (biomedcentral.com)
This bias is even more important when the essentiality of genes is taken into account, and essential genes distribution bias reaches 76% and 94% in Escherichia coli and Bacillus subtilis respectively [ 4 ]. (biomedcentral.com)
For at least a decade now, different insect control strategies that induce RNAi by delivering double stranded RNA (dsRNA) targeting essential genes have been proposed. (cdc.gov)

This study developed a prototype lateral flow immunochromatographic assay (LFIA) employing antibodies against L. monocytogenes Internalin A (InlA) and Internalin B (InlB) proteins, that are involved in non-phagocytic cell invasion. (bvsalud.org)
A portion of this plasmid was derived from a plasmid made by BiFC fragment DNA was generated synthetically to generate peptides described in: Y. Kodama, C.-D. Hu, An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio, BioTechniques. (addgene.org)
This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)

As a postdoctoral researcher in Dianne Newman 's lab at the California Institute of Technology, Dar developed a way to track gene activity across the entire biofilm with submicrometer resolution. (the-scientist.com)
Total biofilm (BF) mass as well as PIA- and protein-mediated biofilm proportions were determined by sodium-periodate and proteinase K treatments, respectively, as explained in Methods. (siamtech.net)

2012). By generating more than one DSB, it becomes possible to produce even more types of changes, including chromosomal deletions, gene inversions and, with DSBs on two different chromosomes, chromosomal translocations (Morgan et al. (baxkyardgardener.com)
The virulence of this bacterium results from the 32 Y pestis chromosomal genes and two Y pestis -specific plasmids, constituting the only new genetic material acquired since its evolution from its predecessor. (medscape.com)

For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
Oxytetracycline has been used to study its resistance gene, otrA and in fluorescence analysis of liver cells. (goldbio.com)
E264 (35) and Bp340 [1026b Δ(E264 included ((((transposon comprising genes from Bp340 (to -kanamycin resistance gene (39) and a hemagglutinin (HA) tag cloned between the XbaI-SacI sites. (healthandwellnesssource.org)

em ssrA /em gene which encodes the tmRNA molecule has been identified in all known bacterial phyla [1,2]. (baxkyardgardener.com)
ABSTRACT The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the blaKPC gene that encodes for carbapenemase production have complicated the management of patient infections. (who.int)

A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and lean twins. (thesdmconsultancy.com)

The final two papers are focused on research in wheat, first led from the University of Leicester that looks at recombination in durum wheat and secondly from Rothamsted in which they have identified a whole family of NPF membrane transporter genes. (garnetcommunity.org.uk)
Christophe Lambing is first author on this study from the Henderson lab at the University of Cambridge that investigates the role of the ASY1 protein in the control of recombination frequency during meiosis. (garnetcommunity.org.uk)
ASY1 is localized in an ascending telomere-to-centromere gradient and this informs the role that it plays to antagonize telomere-recombination to ensure this occurs in more gene-rich regions of the chromosomes. (garnetcommunity.org.uk)
2006. Construction of a 10,000 marker ultradense genetic recombination map of potato: providing a framework for accelerated gene isolation and genomewide physical map . (hutton.ac.uk)
The cluster organization of the NLR gene family could favor sequence exchange between NLR genes via recombination, favoring their evolutionary dynamics. (metasystems-international.com)

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. (goldbio.com)
Some current antibiotics, including a class known as aminoglycosides, work by binding to sites on bacterial ribosomes that are not present on human ribosomes. (scripps.edu)
For six decades, antibiotics have been our bulwark against bacterial infectious diseases, but this bulwark now is collapsing," said Ebright. (phys.org)

ABSTRACT Bacterial cells can vary greatly in size, from a few hundred nanometers to hundreds of micrometers in diameter. (uibk.ac.at)

The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
Using an oligonucleotide-based microarray, we normalized the transcriptomic data by genomic DNA signals in order to have access to inter-gene comparison data. (biomedcentral.com)
Gene duplications increase genetic and phenotypic diversity and occur in complex genomic regions that are still difficult to sequence and assemble. (inra.fr)
There are other duplicated genes in the identified BAC sequences, suggesting that these genomic regions exhibit a high rate of tandem duplication. (inra.fr)

In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms. (genomeweb.com)

This means they regulate genes involved in the same metabolic pathways as the metabolite they bind, which creates regulation through a negative feedback loop. (wikipedia.org)
Metabolic pathways exist for the metabolism of methanol and ethanol within some species of the Pichia taxa to include the yeast of our interest, Pichia pastoris . (igem.org)

After assembly, we performed annotation with the FGENESH program: there were a total of 116 peptides, including 39 PHF7-like proteins identified by BLASTP. (inra.fr)

22 By ligating the app ProR- gfp -reporter gene sequence of pUA321 to pG + host4, the resulting plasmid, pUA625, contained a broad enough host range to transform both gram-negative E. coli and gram-positive B. subtilis . (uark.edu)
The nucleotide sequence of RsaE is usually conserved between both species. (siamtech.net)

This genetic inventory was sufficient to place the cell within the ToL using multigene phylogenetics and provided preliminary insights into the complex evolutionary history of horizontal gene transfer (HGT) in the MAST-4 lineage. (nature.com)
Using these partial data we included the MAST-4 cell in the ToL using multigene phylogenetics and gained insights into its complex evolutionary history of horizontal gene transfer (HGT). (nature.com)
This then injects bacterial proteins into the host cell, which in turn enhance bacterial replication inside that cell. (phys.org)
OmcB,OmcE,OmcS, and MacA are our target genes, since their proteins are the major electron smugglers out of the cell. (igem.org)
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. (downduck.com)

Riboswitches are grouped into classes by the ligand that they bind because the ligand-binding or aptamer domain is highly conserved across species. (wikipedia.org)

Biophotonics researchers at Albert Einstein College of Medicine of Yeshiva University have developed a fluorescent protein that enables scientists to clearly "see" the internal organs of living animals without the need for a scalpel or imaging techniques that can have side effects or increase radiation exposure. (laserfocusworld.com)
The researchers targeted their fluorescent protein to the liver-an organ particularly difficult to visualize because of its high blood content. (laserfocusworld.com)
The findings, according to the researchers, also hint at the existence of unknown RNA assembly factors, most likely proteins, that were not present in their lab-dish-type imaging experiments but are present in cells and boost the efficiency of RNA folding. (scripps.edu)
Most experimental techniques that researchers use to image proteins only work with a defined 3D structure. (mpg.de)

Riboswitches are RNA-based genetic regulatory elements present in the 5' untranslated region (5'UTR) of primarily bacterial RNA. (wikipedia.org)
By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)
Transgenic technology' refers to the transfer of a genetic material from one species to another. (ufl.edu)
However, P. odoriferum shares a vast range of hosts with P. carotovorum , P. versatile , and P. brasiliense , and has similar biochemical, phenotypic, and genetic characteristics to these species. (ppjonline.org)
Knowledge of the genetic makeup of our species, the genetic basis of heritable diseases, and the invention of technology to manipulate and fix mutant genes provides methods to treat diseases. (opentextbc.ca)
Genetic testing is also offered for fetuses (or embryos with in vitro fertilization) to determine the presence or absence of disease-causing genes in families with specific debilitating diseases. (opentextbc.ca)
Gene therapy is a genetic engineering technique that may one day be used to cure certain genetic diseases. (opentextbc.ca)

Some individuals focus on reproduction to expand the colony, while others specialize in construction, oozing polysaccharides and proteins that make up the extracellular matrix. (the-scientist.com)

Once the viruses and their gene cargoes infected liver cells, the infected cells expressed the gene and produced iRFP protein. (laserfocusworld.com)
Other applications that rely on MDA of single cells include targeted metagenomics, whereby marker genes are PCR-amplified from the DNA sample to decipher their distribution in ecosystems or larger fragments of DNA are assembled for analysis of gene content 11 , 17 . (nature.com)
They observed that this system inhibits bacterial escape from host cells, and that normally the SPI-2-encoded T3SS counters this system to facilitate bacterial exit from infected cells. (phys.org)
In supplying the yeast cells with ethanol and methanol simultaneously, the cells will produce the fluorescent protein once the ethanol is utilized. (igem.org)
LA JOLLA, CA - A team of scientists from Scripps Research and Stanford University has recorded in real time a key step in the assembly of ribosomes-the complex and evolutionarily ancient "molecular machines" that make proteins in cells and are essential for all life forms. (scripps.edu)
The team will now be able to extend this research further to study not only the rest of ribosome assembly, which involves multiple RNA strands and dozens of proteins, but also the many other types of RNA-folding and RNA-protein interaction in cells. (scripps.edu)
Transcription is the first step in the process cells employ to read and carry out the out instructions contained in genes. (phys.org)
We propose a model in which a unique fusogenic activity at the C terminus of VgrG5 JWH 133 facilitates intercellular spread by and related species following injection over the plasma membranes of contaminated cells. (healthandwellnesssource.org)

This characterization can be accomplished by fusing app ProR to the gfp- reporter gene, which codes for Green Fluorescent Protein (GFP). (uark.edu)
Characterization of horizontally acquired ribotoxin encoding genes and their transcripts in Aedes aegypti. (cdc.gov)

Ribosomes are made of both RNA and proteins, reflecting a molecular partnership that is widely believed to go back nearly to the dawn of life on Earth. (scripps.edu)
The discoveries significantly advance our understanding of the structure and function of the molecular machine that carries out transcription, setting the stage for new opportunities in combating the bacterial diseases that kill 13 million persons each year worldwide. (phys.org)

Interaction between species also contributes to successful microbial persistence. (asmblog.org)

This shows that we now can examine in detail how RNAs fold while they are being synthesized and proteins are assembling on them," says first author Olivier Duss, PhD, a postdoctoral research fellow in the Department of Integrative Structural & Computational Biology at Scripps Research. (scripps.edu)

Cbl-IIb riboswitches also differ in terms of the nature of the genes they regulate, with Cbl-IIb riboswitches primarily associated with genes involved in ethanolamine utilization. (wikipedia.org)
The six recognized species of Brucella are primarily associated with terrestrial mammals and rodents. (cdc.gov)
To date, gene therapies have been primarily experimental procedures in humans. (opentextbc.ca)

The specific aim of this project was to incorporate the app ProR- gfp -reporter gene complex first into Eschericheria coli ( E. coli ), and then into Bacillus subtilis ( B. subtilis ). (uark.edu)
Those two bacterial species were chosen as hosts for the transformations, for E. coli and B. subtilis are known for being more receptive to recombinant DNA techniques than C. difficile . (uark.edu)
The work provided a detailed look at a complex, and until-now mysterious, part of E. coli ribosome assembly-the formation of an entire major component, or domain, of the E. coli ribosome, with assistance from eight protein partners that end up incorporated into the structure. (scripps.edu)

Salmonella enterica is a major threat to public health, causing systemic diseases (typhoid and paratyphoid fever), gastroenteritis and non-typhoidal septicaemia (NTS) in humans and in many animal species worldwide. (phys.org)

The blaKPC gene was confirmed in 14/20 (70.0%) of these isolates. (who.int)

These organisms are unique in that they are only distantly related to other bacterial sulfate reducers, and because they can grow at extremely high temperatures. (up.ac.za)
This potential pest management method could be easily adapted to target different genes or similar organisms. (cdc.gov)
Nevertheless, this approach provides many drawbacks, like the arbitrary nature from the gene insertion, the chance of disrupting useful genes, public problems over genetically improved organisms (GMOs), as well as the failure to Silmitasertib cost utilize the native hereditary repertoire from the flower. (baxkyardgardener.com)

Thereafter, the specificity of the selected gene was tested through blast analysis. (ppjonline.org)

The asymmetry of the distribution of the genes between the two strands of DNA is explained as being a means of minimizing interruptions in gene transcription due to collisions between the DNA and RNA polymerases. (biomedcentral.com)

In my lab, we are mainly interested how bacterial species grow on plant leaves (the phyllosphere), which growth pattern they exhibit and how they interact with other species that share the leaf with them. (canterbury.ac.nz)
Described in the July 17 online edition of Nature Biotechnology , the new protein could allow doctors, for example, to noninvasively monitor the growth of tumors in order to assess the effectiveness of anti-cancer therapies. (laserfocusworld.com)
This highlights a previously unknown interplay between SPI-2 T3SS and innate immunity in the dynamics of within-host bacterial growth and spread. (phys.org)
We found that the potential for formation of bacterial lipopeptide syringomycin resulted in stronger growth reduction effects on saprophytic Aspergillus nidulans compared to Verticillium spp. (frontiersin.org)
Genes required for degradation of and growth on the carbon source pectin were down-regulated, whereas transcripts involved in redox processes were up-regulated. (frontiersin.org)

Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species. (downduck.com)

In addition, chromosomes with a high number of linked genes that are conserved, even in distant species, were detected. (metasystems-international.com)

Anatomy12

Organisms20

Diseases3

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Psychiatry and Psychology1

Phenomena and Processes76

Disciplines and Occupations5

Technology, Industry, Agriculture5

Information Science5

Health Care5

Confocal laser scan1

Genome23

Microscopy7

Bacteria28

Situ hybridization2

Single-molecule fluorescence1

RRNA5

Strains5

Expression11

Chromosome9

Metabolites2

Meiotic recombination2

Pathogen2

Ribosomal6

Biofilms2

Intracellular3

Infection2

Reactive oxygen1

Jellyfish2

20201

Pathogens3

Arabidopsis2

Fungal2

Genomes3

Vector3

Differentially1

Target bacterial1

20211

Essential genes3

Assay4

Biofilm2

Chromosomal2

Resistance gene3

Encodes2

Bacterium1

Recombination5

Antibiotics3

Abstract1

Genomic4

Promoters1

Metabolic2

BLASTP1

Sequence2

Cell5

Ligand1

Researchers4

Genetic7

Extracellular1

Cells8

Characterization2

Molecular2

Interaction1

RNAs1

Primarily3

Coli3

Diseases1

Isolates1

Organisms3

Specificity1

Strands1

Growth5

Methods1

Chromosomes1