Fluorescence luminescent proteins. Medical search. Frequent questions

Super-resolution microscopy using standard fluorescent proteins in intact cells under cryo-conditions. (ox.ac.uk)
We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, current applications of fluorescence cryo-microscopy to biological specimens achieve resolutions between 400-500 nm only. (ox.ac.uk)
1] Our previous works showed that nonlinear optical properties of ligated gold nanoclusters make them promising candidates for various bio-imaging techniques such as multiphoton excited fluorescence microscopy. (springernature.com)
In vivo vMSOT brain imaging of P301L mice showed higher retention of PBB5 in the tau-laden cortex and hippocampus compared to wild-type mice, as confirmed by ex vivo vMSOT, epi-fluorescence, multiphoton microscopy, and immunofluorescence staining. (springer.com)

We then combined single molecule fluorescence spectroscopy and Amplified Luminescent Proximity Homogeneous Assay Screen to analyse the interactions of subunits of several in vitro reconstituted multisubunit eukaryotic protein complexes such as Cavin, HOPS and Mediator that were previously refractory to reconstitution and analysis. (icam-vn.com)

Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. (intechopen.com)
The purpose of this report is to present an overview of the use of fluorescence imaging in vivo, with particular emphasis on oncology. (duke.edu)
If applied in vivo , this strategy could offer definitive advantages over visible-range fluorescence, particularly the lack of interfering autofluorescence typical for biological molecules. (springernature.com)

Our extensive SpectraMax® line of user-friendly microplate readers - the industry's most cited brand - provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation and include absorbance, fluorescence, and luminescence. (moleculardevices.com)
In our approach, the fluorescence intensity of the reporter dye is related to the luminescence signal of the MLNPs. (cdc.gov)

We tested various antibodies for their ability to neutralize Aß seeds before Aß deposition becomes detectable in Aß precursor protein-transgenic mice. (bvsalud.org)
Organelles can be detected by fluorescence or by detecting epitope tags with antibodies. (riken.jp)
The so-called Fluorescence Quenching Immunoassay uses two antibodies: one analyte specific antibody and one fluorophore specific antibody, whereby binding of the fluorophore specific antibody to the fluorophore reduces the fluorescence intensity. (trea.com)
Abcam: antibodies, proteins, kits. (abcam.com)

To facilitate the identification of potential antivirals, we developed two reporter-expressing ZIKVs, each capable of expressing an enhanced green fluorescent protein or an improved luminescent NanoLuc luciferase. (mdpi.com)
In this study, we genetically modified P. falciparum to simultaneously express GFP, a fluorescent protein, and NanoLuc, a luciferase. (nagasaki-u.ac.jp)
Nano-lantern is a brighter luminescent protein, consisting of luciferase and fluorescent protein. (riken.jp)

Excluding genetic constructs cloning, cell culturing and transfection, the whole protocol may take anywhere from 10 min to several hours, depending on motility of the protein being studied. (nih.gov)
The impact of hantaviral nucleocapsid proteins (N proteins) on motility was examined by transfection of podocytes. (biomedcentral.com)
The impaired motility depends on viral replication and transfection of podocytes with N protein of PUUV or HTNV reveals that the expression of N protein alone is sufficient to deteriorate podocyte function. (biomedcentral.com)

This demonstrates that therapeutically targetable pathogenic Aß seeds already exist during the lag phase of protein aggregation in the brain. (bvsalud.org)
Evidence for GO induced autophagy includes increased levels of isoform B-II microtubule-associated protein 1 light chain 3 (LC3), aggregation of green fluorescence protein-tagged LC3, and increased numbers of autophagic vacuoles in cells. (biomedcentral.com)
A standard approach to visualize aggregation is to fluorescently label the proteins of interest. (lu.se)
2D POLIM was also applied to explore the aggregation state of proteins in the biological system. (lu.se)

The role of the Atorvastatin-β-cyclodextrin complexation in modulating the binding strength of the drug to the model carrier protein bovine serum albumin is studied using absorption and fluorescence spectral measurements and molecular docking. (analchemres.org)
Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron. (ox.ac.uk)
This method can help answer key questions in the plant molecular and cell biology field such as, how can we proteins in the cell. (jove.com)
However, fluorescence brightness alone cannot discriminate micrometer-sized regions with high expression of non-aggregated proteins from regions where the proteins are aggregated on the molecular scale. (lu.se)
As a molecular chaperone, HSP90 regulates the conformational maturation and functional stability of many signaling proteins in cells, serves important roles in cell growth, differentiation, apoptosis and tumor development and is an important antitumor target ( 8 , 9 ). (spandidos-publications.com)

Methods developed to image fluorescence in whole mice have been valuable in studying patterns of tumor growth and metastases. (duke.edu)
To elucidate the dependency of downstream pathologies on Aß, we analyzed the trajectories of cerebral Aß accumulation, Aß seeding activity, and neurofilament light chain (NfL) in the CSF (a biomarker of neurodegeneration) in Aß-precursor protein transgenic mice. (bvsalud.org)
The presence of a LRRK2 protein extra in LRRK2 wild-type and G2019S mice showed exacerbated -synuclein A53T-mediated cytotoxicity. (campaignfornonviolentschools.org)

Applying subcutaneous tumor models in different experimental designs, specific correlations between measured total fluorescence intensity (FI) and the tumor volume (V) could be established. (nih.gov)
The naturally occurring dark state in the parent YFPs yields strong fluorescence modulation upon long-wavelength-induced dark state depopulation, allowing selective detection at the frequency at which the long wavelength secondary laser is intensity modulated. (academic-accelerator.com)

Gel bands or spots were then detected either by one-photon fluorescence (with a fluorescence scanner) or by two-photon excited fluorescence (with a multi-photon confocal microscope). (springernature.com)
Once robust gel protocols were established, we conducted gel imaging with a two-photon confocal microscope (two-photon excited fluorescence, TPEF). (springernature.com)

The microtubule-associated protein tau (MAPT) is located intracellularly and is composed of six isoforms classified into 4-repeat (4R) and 3-repeat (3R) species [ 2 ]. (springer.com)
Western blot was carried out to detect the expression levels of autophagic-related proteins (Unc-51 like autophagy activating kinase 1, beclin 1, autophagy related 5, microtubule-associated protein 1A/1B-light chain 3, ubiquitin-binding protein p62), glycolysis-related proteins (hexokinase 2, phosphofructokinase, pyruvate kinase M2) and activated protein kinase signalling pathway proteins. (ijpsonline.com)

This imaging method assesses homo-FRET between labels by measuring fluorescence polarization in excitation and emission simultaneously, which translates into higher contrast than fluorescence anisotropy imaging. (lu.se)

To address these issues we developed a pipeline for rapid production of recombinant proteins their interaction analysis in high throughput format. (icam-vn.com)
As the first proof of our concept of detection of the protein carbonyls in a native 1D gel, the aminooxy-Au15 NCs were mixed and incubated with oxidized and non-oxidized lysozyme recombinant protein. (springernature.com)
PBB5 showed specific binding to recombinant K18 tau fibrils by fluorescence assay, to post-mortem Alzheimer's disease brain tissue homogenate by competitive binding against [ 11 C]PBB3 and to tau deposits (AT-8 positive) in post-mortem corticobasal degeneration and progressive supranuclear palsy brains. (springer.com)

iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)

Multiplexed immunoassays for proteins using magnetic luminescent nanoparticles for internal calibration. (cdc.gov)
The present invention generally relates to the field of Fluorescence Quenching Immunoassays. (trea.com)
Specifically, the invention provides novel antibody conjugates suitable for use in Discrete Fluorescence Quenching Displacement Immunoassays and methods for producing these antibody conjugates. (trea.com)

There are many different interaction detection methods ranging from those that only identify direct, binary interactions between two proteins (e.g. (stackexchange.com)
AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies. (stratech.co.uk)
a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes). (stratech.co.uk)
Aminooxy-Au15 NCs, to enhance the detection of protein carbonyls by optical methods (see Figure 1). (springernature.com)
By incorporating LNA bases into the (GT)15-DNA sequence, we create sensors that are not only more resistant towards undesirable fluorescence modulation in the presence of Ca2+ but that also retain their capabilities for the label-free detection of dopamine. (academic-accelerator.com)

Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)

The main IRS-1 downstream signaling pathways include type I phosphatidylinositol 3-kinase (PI3K)/Akt (PKB: protein kinase B), mammalian target of rapamycin (mTOR), and mitogen activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK). (biomedcentral.com)

Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems. (intechopen.com)
mPEG-SCM (Amine PEGylation of Proteins) - PEGylation of lysine residues improves the proteolytic stability of fibronectin while retaining biological activity, Biotechnol. (creativepegworks.com)
Proteins carry out and assure the maintenance of all biological functions, therefore there is a paradigm arguing that aging and age-related diseases are the consequences of the accumulation of oxidative damage to proteins. (springernature.com)

Scholars@Duke publication: Intravital fluorescence facilitates measurement of multiple physiologic functions and gene expression in tumors of live animals. (duke.edu)

Here, we demonstrated that CNP activates the cAMP/protein kinase A (PKA) pathway and that this activation contributed to the elongation of the hypertrophic zone in the growth plate. (tokushima-u.ac.jp)
ROS decreased the mammalian target of rapamycin (mTOR)/p70 ribosomal protein S6 kinase signaling, while overexpression of IRS-1 attenuated this inhibition. (biomedcentral.com)
The inhibition effect of ROCOLRRK2 fragments will not be discussed.59 Small Molecule Kinase Inhibitors for LRRK2 LRRK2 is a large protein with several discrete domains. (campaignfornonviolentschools.org)
The mechanism may be related to the improvement of glycolysis dysfunction and alleviation of autophagy over activation of activated protein kinase. (ijpsonline.com)

The capsules were loaded with model proteins such as ovalbumin and green fluorescent protein (GFP), an. (researchgate.net)

This transgenic P. falciparum reporter line constantly expresses two reporter proteins, GFP and NanoLuc, at high levels in two different hosts, human and mosquito. (nagasaki-u.ac.jp)
We have created a transgenic P. falciparum that expresses two reporter proteins, GFP and NanoLuc, at high levels in both the human- and the vector mosquito stage. (nagasaki-u.ac.jp)

Distinct absorption cross-sections for the switching wavelengths in the fluorescent and the nonfluorescent state are not essential for efficient photochromism in fluorescent proteins, although they may facilitate the switching process. (ox.ac.uk)
Your dual-labeled chemical probe for organic application had been assessed by simply Ultra-violet as well as fluorescence to determine its electrochemical absorption as well as engine performance attributes. (hsp70inhibitor.com)

The use of these proteins gives unique opportunities to photolabel and track fusion proteins in a living cell. (nih.gov)
We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. (jove.com)
The main advantage of this technique is the high efficiency of co-expressing cellular fusion proteins in one expression vector. (jove.com)

This book chapter will comment on fluorescent reporter proteins and nanocrystals' applicability as fluorescent markers. (intechopen.com)
By utilising the expression of these two reporter proteins, P. falciparum can be successfully visualised and quantified with high sensitivity. (nagasaki-u.ac.jp)

The insulin receptor substrate (IRS) proteins are a family of cytoplasmic adaptor proteins recognized for their role in insulin signaling. (biomedcentral.com)
Autophagy is a process that relies on lysosomal pathways for the degradation of cytoplasmic proteins and organelles and plays an important role in the pathology of brain injury such as hypoxia ischemia[ 3 ]. (ijpsonline.com)

authors":{"en":[{"name":"Horikawa Kazuki"},{"name":"Nagai Takeharu"}],"ja":[{"name":"堀川一樹"},{"name":"Nagai Takeharu"}]},"description":{"en":"Bioluminescence imaging of cellular function is a promising strategy. (tokushima-u.ac.jp)
To ascertain the cellular goals, any photochemical along with luminescent dimethyl lithopermate W probe was made along with successfully created. (hsp70inhibitor.com)
Indeed, increased protein carbonyl content is a hallmark of cellular and organismal aging. (springernature.com)

Importantly, guest-responsive fluorescence modulation is evidenced through the remarkable turn-off sensing of the pesticide dichloran with admirable selectivity, a quick response time, and a very low LOD (124 nM), with sharp colorimetric changes under UV light. (academic-accelerator.com)
Excited state intramolecular proton transfer (ESIPT) mechanism-based solid state fluorescent materials showed unique photophysical properties such as a large Stokes shift and local environment (pH, polarity, ions and viscosity) responsive fluorescence modulation. (academic-accelerator.com)
The fluorescence modulation was shown to be reversible, with an increase of ≤20% with respect to the original value. (academic-accelerator.com)
Interestingly, we observe very different pKa shift and fluorescence modulation within α-CD compared to β- cyclodextrin (β-CD). (academic-accelerator.com)
The stimuli-induced reversible proton transfer system is composed of materials that release or capture protons in response to stimuli and switch molecules that control color and/or fluorescence modulation by protons, and it is applied in stimuli-responsive materials and devices, including bistable electronic/electrochromic devices, electrofluorochromic devices, water-jet rewritable paper, visible-light-responsive rewritable paper, and mechanochromic materials. (academic-accelerator.com)
For a photochromic spiro-diarylethene derivative (Spiro-DAE), its photocyclization reaction and multicolour fluorescence modulation performances were investigated based on a series of density functionals calculations in this work. (academic-accelerator.com)
This short review is aimed at emphasizing the most prominent recent works devoted to the fluorescence modulation of organic fluorescent or fluorogenic molecules by electrochemistry. (academic-accelerator.com)
These well-controlled fluorescence modulation and photoswitching behaviours not only provide insights into interchromophoric interactions but also contribute to the development of stimuli-responsive luminescent macrocycles. (academic-accelerator.com)

A simple and robust platform for quantitative multiprotein immunoanalysis has been developed with the use of magnetic Co:Nd:Fe(2)O(3)/luminescent Eu:Gd(2)O(3) core/shell nanoparticles (MLNPs) as a carrier. (cdc.gov)
mPEG-NHS (PEGylated Polymers for Gene Delivery) - BSA Nanoparticles for siRNA Delivery: Coating Effects on Nanoparticle Properties, Plasma Protein Adsorption, and In Vitro siRNA Delivery, International Journal of Biomaterials, Volume 2012, Article ID 584060, 10 pages, Text . (creativepegworks.com)
Carbon Dots (CDs) are luminescent quasi-spherical nanoparticles, possessing water solubility, high biocompatibility, and tunable chemical and physical properties for a wide range of applications, including nanomedicine and theranostics. (researchgate.net)

Fluorescence resonance energy transfer (FRET) localizes activated Rac1 in the direction of flow. (ox.ac.uk)

Padron0.9 is a reversibly switchable fluorescent protein with "positive" switching characteristics, exhibiting excellent spectroscopic properties. (ox.ac.uk)
Venus fluorescent protein targeting localization in neurites. (riken.jp)

Indeed, the migration depends only on the size and the charge of the molecules and had to be adapted to reflect the migration of the clusters and the proteins during the same experiment. (springernature.com)

Stably fluorescence protein (FP) expressing cell clones of colorectal carcinoma and germ cell tumor lines were generated by lentiviral transduction using the FPs eGFP, dsRed2, TurboFP635, and mPlum. (nih.gov)
Our microplate readers and software are the industry's most cited and have empowered life science researchers to advance protein and cell biology. (moleculardevices.com)
Hantaviral N protein was detected in tubular and glomerular cells and cell-to-cell contact structures were changed in biopsy samples of patients with serologically confirmed PUUV infections [ 3 ]. (biomedcentral.com)
Gene silencing by hpDNAzymes was assessed by measuring reduced fluorescence from DsRed2 and EGFP reporters in cell culture systems, and reduced 125I-alpha-bungarotoxin binding in cells transfected with cDNA encoding the AChR. (ox.ac.uk)
RESULTS: We show that hpDNAzymes show remarkable resistance to nucleolytic degradation, and demonstrate that in cell culture systems the hpDNAzymes are far more effective than standard 10-23 DNAzymes in down-regulating protein expression from target mRNA species. (ox.ac.uk)
mPEG-MAL (Thiol PEGylation of Proteins) - A Computationally Designed Inhibitor of an Epstein-Barr Viral Bcl-2 Protein Induces Apoptosis in Infected Cells, Cell, Volume 157, Issue 7, 19 June 2014, Pages 1644-1656, Text . (creativepegworks.com)

It has advantages over fluorescence imaging such as high sensitivity, no phototoxicity or no autofluorescence, and compatibility to deep-tissue imaging or optogenetics. (tokushima-u.ac.jp)

mPEG-Maleimide (Thiol PEGylation of Proteins) - In vitro folding of KvAP, a voltage gated K+ channel, Biochemistry. (creativepegworks.com)
A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine) and SiO2 was developed. (researchgate.net)

The magnitude of quenching of the fluorescence of bovine serum albumin due to drug binding, an d the Fӧrster energy transfer efficiency between the protein and the drug are decreased in the presence of β-cyclodextrin. (analchemres.org)

By adding additional unlabeled analyte, via applying a sample, the steady state shifts and more fluorescence signal can be quenched by the fluorophore specific antibody. (trea.com)

Processes such as fluorescence and phosphorescence are examples of intramolecular deactivation processes. (wikipedia.org)

I want to have a network of the interactions between the proteins of these 100 genes. (stackexchange.com)
Thiamine pyrophosphate (TPP) holds in order to thi-box riboswitch RNA along with selleck chemicals llc allosterically prevents body's genes that program code with regard to protein mixed up in the biosynthesis as well as transport of thiamine. (hsp70inhibitor.com)

The brightness of their initial and photoconverted states, along with the high dynamic ranges of both proteins, make them an attractive tool for protein photolabeling. (nih.gov)
Amplify DNA fragments that are necessary for the construction of the semi-independent protein expression cassettes by standard PCR reactions with their corresponding primers and high-fidelity polymerase. (jove.com)

Synergic Bistability between Spin Transition and Fluorescence in Polyfluorene Composites with Spin Crossover Polymers. (academic-accelerator.com)

While the structural and functional information is encoded in a single protein molecule, accessing this information is technically and economically challenging. (icam-vn.com)
mPEG-Maleimide (Thiol PEGylation of Proteins) - Structural and Biochemical Consequences of Disease-Causing Mutations in the Ankyrin Repeat Domain of the Human TRPV4 Channel, Biochemistry, 2012, 51 (31), pp 6195-6206, Text . (creativepegworks.com)

FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)
Currently, protein carbonylation is detected and quantified with 2D gel-based methods using specific fluorescent dyes coupled with hydrazide that reacts with protein carbonyls. (springernature.com)

I have used STRING pretty heavily, and have compared it to various other databases of protein interactions and signaling pathways. (stackexchange.com)

A hallmark of Parkinson's disease is the formation of large protein-rich aggregates in neurons, where α-synuclein is the most abundant protein. (lu.se)

Yeast two-hybrid ), those that also find proteins that are in the same complex even if there is no direct interaction between them (e.g. (stackexchange.com)

mPEG-Alkyne (Clickable PEGylation of Proteins) - Visualization of O" GlcNAc Glycosylation Stoichiometry and Dynamics Using Resolvable Poly(ethylene glycol) Mass Tags, Current Protocols in Chemical Biology, 5:281-302, Text . (creativepegworks.com)

The concept is demonstrated for a simultaneous immunoassay for three model proteins (human, rabbit, and mouse IgGs). (cdc.gov)
The present study aimed to investigate the effects of changes in heat shock protein (HSP)90β expression and verify whether HSP90β regulates EAAT2 expression in a cerebral ischemia‑reperfusion injury model. (spandidos-publications.com)

Chemicals and Drugs18

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