• In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. (uni-goettingen.de)
  • In addition, using Congo red and Thioflavin T binding assays and negative-staining transmission electron microscopy, we show that the W proteins phase separate to form amyloid-like fibrils. (sciencegate.app)
  • This course is on advanced fluorescence microscopy imaging and spectroscopy techniques for quantitative characterization of molecular transport and interactions in cells. (ki.se)
  • Fluorescence microscopy and associated techniques are indispensable research tools for investigating molecular mechanisms of biological processes. (ki.se)
  • Versatility of fluorescence microscopy based techniques comes from the possibility to characterize fluorescence emission by spatial position, intensity, wavelength, lifetime and polarization. (ki.se)
  • In addition, fluorescence microscopy and spectroscopy based techniques allow us to quantitatively study the cellular dynamics of molecules and the kinetics of their interaction with high spatio-temporal resolution and ultimate, single-molecule sensitivity. (ki.se)
  • Spatially resolved fluorescence imaging: Multi-photon excitation, Total Internal Reflection Fluorescence (TIRF) Microscopy, Super-resolution techniques (STORM, PALM and STED). (ki.se)
  • Using X-ray fluorescence microscopy, transmission and scanning electron microscopy, and biological function assays, we tested the hypothesis that zinc release contributes to ZP hardening. (northwestern.edu)
  • In this study, we observed bacterial response to CBD exposure through biological assays, bacterial kinetics, and fluorescence microscopy. (veterinarycannabissociety.org)
  • Then, physicochemical characterisation methods such as XPS and fluorescence confocal microscopy were carried out on films together with in vitro cell-based assays. (upc.edu)
  • The proposal will apply an interdisciplinary approach in an international environment to maximize its chances of success: the biological role of reading domains will be evaluated with molecular biology (histone and chromatin pulldowns, ChIP-seq, confocal microscopy), biochemistry (Peptide arrays, mass spectrometry, methyltransferase activity assays) and biophysics techniques (fluorescence anisotropy, circular dichroism spectroscopy) as well as crystallography (solving the structure of reading domains). (europa.eu)
  • Correlation of in vitro transcription inhibition activity with in vivo mechanism of action was established using fluorescence microscopy and resistance passaging using Gram-positive bacteria showed no resistance development over 30 days. (edu.au)
  • Adhesion assays, time-lapse video microscopy and morphological examination utilising immunohistochemistry techniques in combination with fluorescence microscopy and scanning electron microscopy were applied to investigate behavioural alterations. (gla.ac.uk)
  • Molecular diagnostics uses in vitro biological assays such as PCR-ELISA or Fluorescence in situ hybridization. (wikipedia.org)
  • in vivo) activity per unit particle surface area showed that all in vitro assays correlated significantly with in vivo results, with the cellular assays correlating the best. (cdc.gov)
  • Osmolarity and peroxidation in both formulations was similar and the in vitro drug release assay indicated Quisinostat minimal release (2 %) of the doxorubicin content after 48 h. (dna-pkinhibitors.com)
  • Synthesis, in vitro transcription inhibition assays, and biological testing of the hybrids identified a range of potent anti-transcription inhibitors with activity against a range of pathogenic bacteria with MICs as low as 3.1 μM. (edu.au)
  • Fluorescent labels are stable for months and deliver high sensitivity, and the diversity in available dyes gives nearly unlimited possibilities in assay design. (labonline.com.au)
  • Learn how to master the challenges of cell-based fluorescence assays and improve the quality and sensitivity of your assays. (labonline.com.au)
  • The sensitivity of this assay makes it highly useful for hit finding and validation in drug discovery. (uzh.ch)
  • Time-resolved fluorescence provides high sensitivity for biological and cellular assays, even when sample is at a premium or in low concentration. (petroemphor.com)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds. (anaspec.com)
  • The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds, resulting in a high signal to background ratio. (hum-molgen.org)
  • This was supported by FMA, which showed a relative fluorescence intensity of ca. 3815% for the nanosystem, concerning bare QDs or EcTI alone. (bvsalud.org)
  • The resulting fluorescence intensity (λex/em = 530/585 nm) is linear to the L-phenylalanine concentration in the sample. (bioassaysys.com)
  • The Transcreener FI ADP Assay Kit produces a simple fluorescent intensity output which can be read on even the most basic fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers. (bellbrooklabs.com)
  • FLIP (FLuorescence IP) relies on the expression of the target protein as a chromophore-tagged protein and couples IP with the measurement of fluorescent signal coating agarose beads. (biomedcentral.com)
  • In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)
  • In the assay, L-phenylalanine is oxidized by phenylalanine dehydrogenase, producing NADH, which reduces a fluorescent dye to a highly fluorescent product. (bioassaysys.com)
  • The Transcreener ® ADP 2 Assay Kit with FI readout measures ADP via antibodies selective to ADP and a fluorescent tracer. (bellbrooklabs.com)
  • In this study, we have evaluated and validated a two-component direct fluorescent-antibody (DFA) assay, using the monoclonal immunoglobulin (Ig) M antibody EAII-6G6-2-3 against the cell wall polysaccharide antigen (CW) ( 12 ) and the monoclonal IgG antibody FDF-1B9 against the capsule antigen (CAP) ( 16 ) for rapid identification of B. anthracis . (cdc.gov)
  • Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine optimal filters and settings for maximal assay performance. (bellbrooklabs.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • AnaSpec also offers a shorter wavelength thrombin assay, which employs an AFC (7-amido-4-trifluoromethylcoumarin) labeled substrate, the SensoLyte AFC Thrombin Activity Assay Kit. (hum-molgen.org)
  • Data from this study indicate that it is possible to predict acute in vivo inflammatory potential of NP with cell-free and cellular assays by using NP surface area-based dose and response metrics, but that a cellular component is required to achieve a higher degree of predictive power. (cdc.gov)
  • Cell-based assays are giving us deeper insight into cellular mechanisms in a true biological context, and fluorescence assays are playing a leading role. (labonline.com.au)
  • To further characterize the parental compound and its analogues, we developed a high-speed, low-cost, quantitative fluorescence quenching assay. (uzh.ch)
  • Molecular diagnostics is a collection of techniques used to analyze biological markers in the genome and proteome, and how their cells express their genes as proteins, applying molecular biology to medical testing. (wikipedia.org)
  • Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
  • Lysine methylation marks manifest their biological effect via so-called 'readers' (or reading domains) which recognize and bind the methylation mark and directly alter the chromatin structure or act as a scaffold for other proteins, which induce biological responses. (europa.eu)
  • Fluorescence-based surface markers can also be used to identify other factors of importance to the line of cells being produced, like the location of proteins on the cell, for example. (techcrunch.com)
  • Proteomics: LC-MS/MS can be used for the identification and quantification of proteins in complex biological samples, such as blood or tissue samples. (bmdrc.org)
  • However, finding a generic method that is applicable to membrane proteins has been a challenge as the high hydrophobicity of membrane proteins and the presence of detergents essential for their solubilization interfere with fluorescence-based detections. (nih.gov)
  • We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)
  • This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. (biolegend.com)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • Both DFA assays tested positive on 14 of 26 clinical specimens from the 2001 anthrax outbreak investigation. (cdc.gov)
  • The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens. (cdc.gov)
  • Earlier studies demonstrated the advantages of immunofluorescence assays, based on polyclonal antibodies to B. anthracis cell-surface antigens, for identifying B. anthracis isolates ( 8 ) and directly evaluating clinical specimens from infected guinea pigs ( 9 ). (cdc.gov)
  • In addition to use on isolates, this rapid DFA assay was applied successfully to detect B. anthracis directly in clinical specimens from several patients with laboratory-confirmed inhalational anthrax during the 2001 bioterrorism-associated anthrax outbreak in the United States ( 6 , 17 ). (cdc.gov)
  • There are no specific clinically validated assays for detection of ricin that can be performed by the hospital/healthcare facility clinical laboratory. (cdc.gov)
  • The assay detects a molecule, often in low concentrations, that is a marker of disease or risk in a sample taken from a patient. (wikipedia.org)
  • The SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. (anaspec.com)
  • Xenopus is increasingly being used as imaging test-bed to investigate the roles of cytoskeleton and intracellular trafficking in cell biological and morphogenetic contexts. (cshl.edu)
  • Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. (tocris.com)
  • Two cell-free and two cell-based assays were evaluated for their power in predicting in vivo toxicity of eight distinct particle types with widely differing physicochemical characteristics. (cdc.gov)
  • BioAssay Systems' L-Phenylalanine Assay Kit provides a convenient fluorimetric means to measure L-phenylalanine in biological samples. (bioassaysys.com)
  • The cell-free systems comprised fluorescence- and electron spin resonance-based assays of oxidant activity. (cdc.gov)
  • Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope. (tocris.com)
  • The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. (anaspec.com)
  • It is a simple one-step homogenous detection assay, and accommodates ATP concentrations from 0.1 to 100 µM ATP. (bellbrooklabs.com)
  • We describe a computational methodology allowing to systematically and quantitatively characterize a Boolean mathematical model of a biological network in terms of genetic interactions between all loss of function and gain of function mutations with respect to all model phenotypes or outputs. (researchgate.net)
  • Increase of fluorescence can be monitored at the commonly used excitation and emission wavelengths of 490 nm and 520 nm, respectively. (hum-molgen.org)
  • The immunoprecipitation (IP) assay is a valuable molecular biology tool applied across a breadth of fields. (biomedcentral.com)
  • The industrialisation of molecular biology assay tools has made it practical to use them in clinics. (wikipedia.org)
  • Although data concerning the potential toxicity of some NP have been reported, validated simple assays are lacking for predicting their in vivo toxicity. (cdc.gov)
  • The aim of this study was to evaluate new response metrics based on chemical and biological activity of NP for screening assays that can be used to predict NP toxicity in vivo. (cdc.gov)
  • This demonstrates that the currently accepted toxicity assays are poor predictors of the longer-term biological performance of a material. (birmingham.ac.uk)
  • This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. (anaspec.com)
  • A scratch wound-healing assay was used to detect the migration of VSMCs, and immunocytochemistry and western blotting for SM22ɑ were used to evaluate phenotypic transformation. (hindawi.com)
  • Forensic analysis: LC-MS/MS can be used to detect and quantify drugs and other illicit substances in biological or environmental samples, such as blood or urine, and to provide evidence in criminal investigations. (bmdrc.org)
  • In previous courses, we have guided students in the ablation of a wide variety of genes and helped them design suitable assays for their biological interests. (cshl.edu)
  • The Mouse Proinflammatory Chemokine Panel 2 (2-plex) is a multiplex bead-based assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. (biolegend.com)
  • AnaSpec has announced the launch of the industry s first FRET-based thrombin assay the SensoLyte 520 Thrombin Activity Assay Kit. (hum-molgen.org)
  • The SensoLyte 520 Thrombin Activity Assay Kit kit employs a novel internally quenched 5-FAM/QXL 520 FRET substrate (Figure 1). (hum-molgen.org)
  • The aim of this thesis was to realize single cell isolation by means of automated immunomagnetic bead enrichment and antibody-based fluorescence detection in a chip-based microfluidic system and the molecular biological characterization of these cells to assay tumor features from liquid biopsy. (fraunhofer.de)
  • Enzymatically generated ADP displaces the tracer from an antibody-quencher conjugate, resulting in increased fluorescence. (bellbrooklabs.com)
  • Sufficient antibody is included in the kit to complete 200 assays (Part #3013-A ), 1,000 assays (Part #3013-1K ), or 10,000 assays (Part #3013-10K ) at an ATP concentration up to 100 µM. (bellbrooklabs.com)
  • 2. Understand the buildup of fluorescence imaging instrumentation, identify different optical elements and describe their function. (ki.se)
  • It can be used to identify and quantify metabolites in different biological systems and to study their role in biological processes. (bmdrc.org)
  • Overall, LC-MS/MS is a powerful and versatile analytical tool that can be applied in many different fields to study a wide range of biological and environmental processes. (bmdrc.org)
  • Tissue culture is one of the most widely used techniques in any biological or biomedical research lab. (stoodnt.com)
  • A main challenge of Tissue engineering is to create sustainable scaffolds that provide optimal biological properties to mimic the extracellular matrix(ECM). (upc.edu)
  • Specific compounds detected in the binding assay were further analyzed by competitive ITC, NMR, and surface plasmon resonance, and the assay's utility in comparison with these biophysical methods is discussed. (uzh.ch)
  • Some of the common methods available for determining cyanide in biological media are reported in Table 7-1. (cdc.gov)
  • Al- direct demonstration or cultivation of the though a number of papers have reported causative organism, remain the definitive the existence of circulating antigens and laboratory diagnostic method for VL, sero- immune complexes in VL [ 16-19 ], none of diagnostic methods are needed to over- them has led to a useful antigen detection come the diagnostic difficulties assay for VL. (who.int)
  • This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)
  • Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples. (bellbrooklabs.com)
  • The panel has been validated by detecting expected changes in biological samples. (biolegend.com)
  • Metabolomics: LC-MS/MS can be used to analyze small molecules, such as metabolites, in biological samples. (bmdrc.org)
  • The potential uses of tests for either ricin or ricinine in human biological samples would be primarily for purposes of confirming exposure or assessing the prevalence of exposure rather than diagnostic use. (cdc.gov)
  • The efficiency sential y uniform after whole-body izing (i.e. removing electrons from) in causing damage and subsequent exposure to being highly heteroge- atoms or molecules of the medium biological effects is related not only neous in the case of non-uniform to the amount of energy transferred being traversed. (who.int)
  • Safety assessment of these materials traditionally relies on the use of simple two-dimensional assays, where cells are grown on the surface of the material over a relatively short time frame. (birmingham.ac.uk)
  • The technique helps view cell pictures in 2D by stacking the 1D spatial profiles after demodulating the detected transmission and fluorescence signals. (grandviewresearch.com)
  • We validated the assay using single Trp-to-Ala protease mutants and the competitive protease inhibitor aprotinin. (uzh.ch)
  • Serine proteases play crucial biological roles and have their activity controlled by inhibitors, such as the EcTI, a serine protease inhibitor purified from Enterolobium contortisiliquum seeds, which has anticancer activity. (bvsalud.org)
  • Concluding, the QD-EcTI represents a valuable nanotool to study the interaction of this inhibitor with cancer cells using fluorescence-based techniques with the potential to unravel the intricate dynamics of interplays between proteases and inhibitors in cancer biology. (bvsalud.org)
  • These techniques bring new biological insight at an unprecedented rate and are of crucial importance for the development of life sciences. (ki.se)
  • We make use of biochemical and cell biological techniques, such as radioimmunoassay, high pressure liquid chromatography, fluorescence immunocytochemistry and receptor binding assays, in order to examine the trafficking of the mutant p roinsulins. (nih.gov)
  • Over the years, cell sorting was combined with molecular biological techniques and proteomics for characterizing metastatic & primary cancer cell populations and single tumor cells. (grandviewresearch.com)
  • In the first case study, we observed that the real biological networks were most sensitive to overexpression/state-flip and edge-addition/-reverse mutations among node-based and edgetic mutations, respectively. (researchgate.net)
  • Fluorescence images and biological assays showed a decrease in cell proliferation and viability with an increase in cobalt concentration. (birmingham.ac.uk)
  • The power of Xenopus can be leveraged when live-cell fluorescence imaging is combined with microsurgery, grafting, and dissociated cell culture. (cshl.edu)
  • By using machine learning and AI to do the identification based on standard and well-accepted biological assays used by the FDA, the system could move away from human annotation and the variability that introduces into the process of human cell production. (techcrunch.com)
  • On the basis of technology, Fluorescence-based Droplet Cell Sorting (FACS) segment generated the highest revenue in 2018, as it is a highly accurate and precise sorting method. (grandviewresearch.com)
  • Concept of assessing nanoparticle hazards considering nanoparticle dosemetric and chemical/biological response metrics. (cdc.gov)
  • In contrast, nonspecifically binding compounds did not quench its fluorescence and showed similar IC(50) values with steep dose-response curves. (uzh.ch)
  • The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener assays perform optimally on your specific instrument. (bellbrooklabs.com)
  • We observed that specific analogues quenched dengue protease fluorescence and showed variation in IC(50) values. (uzh.ch)
  • However, if you don't have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. (biolegend.com)
  • The finished assay can be acquired on a variety of dual-laser flow cytometers and analyzed using FCAP Array™ Software or directly with FlowJo™ v10 Software using a downloadable plug-in. (bdbiosciences.com)
  • Flow cytometry-based assay help quantifies and characterizes infected cells in HIV patients. (grandviewresearch.com)
  • Biological activity of ABD-GCSF protein was assessed by proliferation assay on NFS-60 cells. (sciencegate.app)
  • 3. Describe the theoretical background behind specialized fluorescence based methodologies for studying molecular interactions in live cells. (ki.se)
  • However, filaments results being unreliable, further characterisation technics such as radiolabelling, radioactivity or biological assays with human cells have to be carried out to corroborate the results of this project. (upc.edu)
  • The defense of his dissertation on "Automated Isolation of Circulating Tumor Cells and their Molecular Biological Analysis" took place on January 30, 2023 at the Johannes Gutenberg University Mainz. (fraunhofer.de)
  • The immunoprecipitation assay (IP) is an important tool used in academic and industrial research labs for applications such as targeted protein purification, protein concentration, analysis of protein-protein interactions, identification/analysis of protein complexes and analysis of protein/DNA interactions using chromatin IP (ChIP). (biomedcentral.com)
  • Microsomal stability assay (mouse, human). (bmdrc.org)
  • This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • This assay is designed to be used with purified enzyme preparations. (bellbrooklabs.com)
  • Transcreener ® ADP 2 Assay Kits are designed for use with purified enzyme preparations. (bellbrooklabs.com)
  • There may be a decrease in signal, but overall, the assay results should not be affected. (biolegend.com)