• fluorescence
  • In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. (abcam.com)
  • It involves administration of sodium fluorescein into the systemic circulation, and then an angiogram is obtained by photographing the fluorescence emitted after illumination of the retina with blue light at a wavelength of 490 nanometers. (wikipedia.org)
  • Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. (wikipedia.org)
  • proteins
  • Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing biologists to target the fluorophore to specific proteins or structures within cells. (wikipedia.org)
  • probes
  • Fluorescein-labelled probes can be imaged using FISH, or targeted by antibodies using immunohistochemistry. (wikipedia.org)
  • FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the complementary nucleic acids or primers for polymerase chain reaction. (wikipedia.org)
  • primary
  • Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining. (vectorlabs.com)
  • A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. (wikipedia.org)
  • product
  • This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. (abcam.com)
  • solution
  • In pure state, fluorescein is a red or orange powder which is dissolved in alkaline solutions to make up a yellow solution which will glow green. (wisegeek.com)
  • The color of its aqueous solution varies from green to orange as a function of the way it is observed: by reflection or by transmission, as can be noticed in bubble levels, for example, in which fluorescein is added as a colorant to the alcohol filling the tube in order to increase the visibility of the air bubble contained within (thus enhancing the precision of the instrument). (wikipedia.org)
  • The Confederation negotiated a solution to the difficulties posed by the use of fluorescein impregnated paper strips when Bausch & Lomb discontinued the production of Fluorets. (wikipedia.org)
  • uses
  • One of its more recognizable uses was in the Chicago River, where fluorescein was the first substance used to dye the river green on St. Patrick's Day in 1962. (wikipedia.org)
  • Other uses of fluorescein include using it as a water-soluble dye added to rainwater in environmental testing simulations to aid in locating and analyzing any water leaks, and in Australia and New Zealand as a methylated spirit dye. (wikipedia.org)
  • flow
  • Oceanographers and hydrologists have historically used fluorescein to trace the flow of currents through bodies of water of interest. (wisegeek.com)
  • In addition to providing data about current flow, the movement of the fluorescein can also be used to trace contaminants, by tagging the contaminant with the compound and watching to see where it goes. (wisegeek.com)
  • active
  • Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. (netdoctor.co.uk)
  • Fluorescein dye solutions, typically 15% active, are commonly used as an aid to leak detection during hydrostatic testing of subsea oil and gas pipelines and other subsea infrastructure. (wikipedia.org)
  • group
  • After the first group of pictures is taken, a dye called fluorescein is injected into a vein. (scripps.org)
  • After the group's demise, Van Leeuwen and Scott went on to tour with the band Failure while Rubenstein joined the group Fluorescein. (wikipedia.org)