ProteinsSodiumDimensional Polyacrylamide Gel ElectrophorCapillarySynovial fluidsLiquid chromatographyCerebrospinal fluidChromatographyAgaroseAscitic FluidBody FluidsProteinMolecular weightSupernatantPolymersSieveIonsBufferAnalysisPancreaticHumanDiagnosticMoleculesPAGEPreparationWesternTherapyUnitTechniquesMatrixSamplesChangeResearchPresentContentCells
Proteins17
- In this study, we characterized glycoproteins and nonglycoproteins on pooled mucinous (n = 10) and nonmucinous (n = 10) pancreatic cyst fluid to identify "proteins of interest" to differentiate between mucinous cyst from nonmucinous cyst and investigate these proteins as potential biomarker targets. (nih.gov)
- Boiled, fried, and roasted peanut proteins were subjected to digestion in a Simulated Gastric Fluid (SGF), and surviving allergenic fragments were using human serum IgE and other antibodies. (usda.gov)
- Boiled, fried, and roasted peanut proteins were subjected to digestion with pepsin in a Simulated Gastric Fluid (SGF), and were then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and Western Blot analysis using human serum IgE and specific anti-Ara h 1 and Ara h 2 antibodies. (usda.gov)
- The discovery of this homologue in tears offers the potential for disease diagnosis by screening tear fluid proteins. (edu.au)
- Studies on the Proteins of Various Body Fluids in the Field of Obstetrics by Means of DISC-Electropheresis (Polyacrylamide-Gel Electrophoresis) Part 1. (yamaguchi-u.ac.jp)
- Unlike Sandia's MicroChemLab - its patented version of a lab on a chip - which reports multiple protein signatures in fluids of interest, the clinical diagnostic instrument described in PNAS is a lab on a chip designed to quantify the amount of a specific protein (or panel of proteins) present in particular biological fluids. (sandia.gov)
- A low electrical current (measured in micro-amps) is passed through the gel and a process called electrophoresis moves charged proteins through it. (sandia.gov)
- Analysis of three tear proteins using gel electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis) was done at baseline, at the first and second post-treatment visits. (drcremers.com)
- a) Vertical Gel Apparatus: It is used for the separation of proteins in SDS-PAGE. (slideshare.net)
- High resolution two-dimensional electrophoresis of proteins. (pdbwiki.org)
- A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. (pdbwiki.org)
- 10: Sheen H. Quantitation of yeast total proteins in sodium dodecyl sulfate- polyacrylamide gel electrophoresis sample buffer for uniform loading. (moleculardepot.com)
- SDS PAGE electrophoresis is a widely used technique for separating and analyzing proteins based on their molecular weight. (heathnewsonlines.net)
- It involves using sodium dodecyl sulfate (SDS) to denature and uniformly charge the proteins, followed by electrophoresis through a polyacrylamide gel matrix. (heathnewsonlines.net)
- In SDS PAGE electrophoresis, the protein sample is mixed with an SDS-containing buffer, denatures the proteins, and imparts a uniform negative charge. (heathnewsonlines.net)
- The polyacrylamide gel matrix acts as a sieve, with smaller proteins migrating faster and farther through the gel than larger proteins. (heathnewsonlines.net)
- After electrophoresis, the proteins can be visualized using staining techniques, such as Coomassie Blue or silver stain, and identified using mass spectrometry or other analytical methods. (heathnewsonlines.net)
Sodium2
- FDPs were immunopurified from individual OCAF samples, characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis /western blots, enzyme-linked immunosorbent assays, and CAM assays. (mcmaster.ca)
- Purity of vaccines was also determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). (academicjournals.org)
Dimensional Polyacrylamide Gel Electrophor1
- An analytical reference map of normal human reflex tears was created using two- dimensional polyacrylamide gel electrophoresis (2-D PAGE) with pH 3.5-10 immobilized pH gradients (IPGs). (edu.au)
Capillary4
- Novotny was recognized for the development of PAGE Polyacrylamide Gel-filled Capillaries for Capillary Electrophoresis in 1993. (wikipedia.org)
- Most notably, Milos has worked a great deal with microcolumn separation techniques of liquid chromatography, supercritical fluid chromatography, and capillary electrophoresis. (wikipedia.org)
- 4. And a support for the buffer such as filter paper, cellulose acetate strips, polyacrylamide gel, or a capillary tube. (slideshare.net)
- The presence of HMW species, for instance, is frequently determined using size-exclusion chromatography (SEC) coupled with high-pressure liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC), while LMW species are detected using a capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) (reduced and non-reduced) technique. (pharmtech.com)
Synovial fluids2
Liquid chromatography1
- A multidimensional differential proteomic platform using dual-phase ion-exchange chromatography-polyacrylamide gel electrophoresis/reversed-phase liquid chromatography tandem mass spectrometry. (ufl.edu)
Cerebrospinal fluid6
- The major apolipoproteins (apo) on human cerebrospinal fluid lipoproteins are apoA-I and apoE. (elsevierpure.com)
- Given the association between inheritance of the ε4 allele of the apoE gene (APOE4) and increased susceptibility to Alzheimer's disease, we tested the hypothesis that cerebrospinal fluid apolipoproteins may be influenced by APOE genotype and Alzheimer's disease. (elsevierpure.com)
- 1.210 g/ml) were isolated from cerebrospinal fluid obtained from individuals with different APOE genotypes and with or without pathologically verified Alzheimer's disease. (elsevierpure.com)
- These differences in cerebrospinal fluid apolipoproteins may influence lipoprotein trafficking and may be an element in the stratification of risk for Alzheimer's disease with APOE genotype. (elsevierpure.com)
- Cerebrospinal Fluid Levels of Kininogen-1 Indicate Early Cognitive Impairment in Parkinson's Disease Background: Cognitive impairment is common in patients with PD. (pdbwiki.org)
- Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients. (ufl.edu)
Chromatography3
- Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (lookformedical.com)
- Both hybrid antibodies were purified from ascitic fluid by size exclusion chromatography. (docksci.com)
- Purified MAV-2(O) contained 3- to 4-fold higher levels of gp85 than AMV, as determined by polyacrylamide gel electrophoresis and guanidine hydrochloride column chromatography. (uky.edu)
Agarose2
- A quantidade de glicosaminoglicanos (GAGs) extraídos da cartilagem articular dos côndilos femorais foi medida após eletroforese em gel de agarose. (usp.br)
- Cartilage extracted glycosaminoglycans (GAGs) concentration was assessed after agarose gel electrophoresis. (usp.br)
Ascitic Fluid1
- In this work, we used cytotoxicity and enzymatic assays and morphological analysis to examine the effects of a proteolysis-inducing factor (PIF)-like molecule purified from ascitic fluid of Walker tumour-bearing rats (WF), which has been suggested to be responsible for muscle atrophy, on cultured C 2 C 12 muscle cells. (biomedcentral.com)
Body Fluids1
- It is a separation technique, easy, simple and highly sensitive test and used on body fluids such as blood serum ,urine and CSF. (slideshare.net)
Protein1
- In addition to their expertise in SDS PAGE electrophoresis, Kendrick Labs offers various other analytical services, including 2D gel electrophoresis, mass spectrometry, and protein sequencing. (heathnewsonlines.net)
Molecular weight2
- GAGs' molecular weight was evaluated after polyacrylamide gel electrophoresis. (usp.br)
- Mucinases are enzymes capable of degrading mucins (the complex high molecular weight molecules that are the major non-aqueous components of mucous gels). (bmj.com)
Supernatant2
- gp85, Drs. D. P. Bolognesi and J. Li for anti-gp85 sera, and Dr. A. Langlois for AMV-containing supernatant fluids. (uky.edu)
- 12,000 g ) and add ammonium sulphate to 20% (w/v), or PEG, M. Wt 6000, to 6% (w/v) to the supernatant fluid. (dpvweb.net)
Sieve1
- Using a disposable lab-on-a-chip cartridge, the device makes use of a molecular sieve made out of a polyacrylamide gel. (sandia.gov)
Ions1
- In the electric field the negative end pushes the ions or molecules through the gel, and a positive end pulls the ions or molecules through the gel. (slideshare.net)
Buffer2
- The buffer capacity of single- site, resting, human dental-plaque fluid. (moleculardepot.com)
- M. Optimization of buffer solutions to analyze inflammatory cytokines in gingival crevicular fluid by multiplex flow cytometry. (moleculardepot.com)
Analysis1
Pancreatic1
- chlorophenylalanine (PCPA), a serotonin depletor, profoundly increased pancreatic fluid and bicarbonate secretion but remarkably inhibited pancreatic amylase secretion in anesthetized rats. (kyobobook.co.kr)
Human1
- In 1955, Oliver Smithies found that separation of human tissue extracts with high resolution by starch gel electrophoresis. (slideshare.net)
Diagnostic1
- We have on-going efforts to use the diagnostic platform to detect biotoxins and other markers in bodily fluids to be able to diagnose exposure to a biological agent. (sandia.gov)
Molecules2
- The different sized molecules form distinct bands on the gel. (slideshare.net)
- Mucous gels exhibit non-specific antibacterial capabilities as they contain molecules such as lactoferrin, lysozyme, and immunoglobulins. (bmj.com)
PAGE6
- C. Thin layer Electrophoresis and D. Gel Electrophoresis (SDS, PAGE, etc..) 2. (slideshare.net)
- As in SDS-PAGE (polyacrylamide gel electrophoresis), the LMWs are separated based on size. (pharmtech.com)
- Kendrick Labs is a premier provider of SDS PAGE electrophoresis services, offering high-quality results and customized solutions to meet the unique needs of researchers and organizations. (heathnewsonlines.net)
- How Does SDS PAGE Electrophoresis Work? (heathnewsonlines.net)
- Why Choose Kendrick Labs for SDS PAGE Electrophoresis? (heathnewsonlines.net)
- Kendrick Labs is a premier provider of SDS PAGE electrophoresis services, with a team of experienced scientists and technicians who are experts in the field. (heathnewsonlines.net)
Preparation1
Western2
- Apolipoproteins were separated by SDS-polyacrylamide gel electrophoresis and identified by silver nitrate staining, Western blotting, and N-terminal amino acid sequencing. (elsevierpure.com)
- Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
Therapy1
- Oral rehydration therapy (ORT) is the preferred treatment for fluid and electrolyte losses due to diarrhoea in children with mild to moderate dehydration. (bvsalud.org)
Unit1
- Components of Electrophoresis unit 1. (slideshare.net)
Techniques1
- In between 1950 to 1970, an enumeration of techniques and instrumentation for Electrophoresis were developed. (slideshare.net)
Matrix1
- The model is then loaded into wells on a polyacrylamide gel matrix placed in an electrophoresis chamber. (heathnewsonlines.net)
Samples1
- Samples were collected after written consent was giv- nization Program and, after March 2006, became available en by the parents. (cdc.gov)
Change1
- It was also revealed in SDS-polyacrylamide gel electrophoresis that the qualitative change of amylase was induced by PCPA. (kyobobook.co.kr)
Research2
- Because the amount of sample fluid needed for testing is so small, Amy sees further applications in other disease areas - including potentially improved diagnosis of prostate and breast cancer - as well as rapid measurements of serum in animal models employed in vaccine development research. (sandia.gov)
- The work, funded by the National Institute of Dental and Craniofacial Research (NIDCR) - one of 20 institutes in the National Institutes of Health - is the first application using microliters of saliva, a painlessly and easily secured fluid. (sandia.gov)
Present2
- The present study revealed that SALSA was present in the amniotic fluid (AF) and exceptionally enriched in both meconium and feces of infants. (researchgate.net)
- Consequently, the objective of the present study was to investigate the effect of EW on the gel-forming ability of rohu (Labeo rohita). (scirp.org)
Content1
- Non-toxic low sugar content so suitable for electrophoresis of polysaccharides. (slideshare.net)
Cells1
- Dysregulation of the NF-κB pathway in B cells from PPARγ +/- was indicated by spontaneous NF-κB activation, as well as increased IκBα phosphorylation and gel-shift activity following LPS stimulation. (jci.org)