• cytometer
  • A flow cytometer allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. (wikipedia.org)
  • A common variation involves linking the analytical capability of the flow cytometer to a sorting device, to physically separate and thereby purify particles of interest based on their optical properties. (wikipedia.org)
  • The first label-free high-frequency impedance flow cytometer based on a patented microfluidic "lab-on-chip", Ampha Z30, was introduced by Amphasys (2012). (wikipedia.org)
  • At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to commonly use the name "flow cytometry", a term that quickly became popular. (wikipedia.org)
  • A flow cytometer is similar to a microscope , except that, instead of producing an image of the cell, flow cytometry offers high-throughput, large-scale, automated quantification of specified optical parameters on a cell-by-cell basis. (wikipedia.org)
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (wikipedia.org)
  • Thousands of cells can be analyzed by a flow cytometer in a single second. (scripps.edu)
  • The development of a specialized flow cytometer in the 1970s, triggered by the HIV pandemic, paved the way to a broad range of applications such as protein modifications studies, immunophenotyping, and analysis of intracellular antigens. (news-medical.net)
  • The SOEST Flow Cytometry Facility in the Pacific Ocean Sciences and Technology Building, Room 20 (POST 20) has two flow cytometers: one is a research-grade flow cytometer capable of sorting cells (Beckman-Coulter Altra) and the other is a bench top analyzer (Beckman-Coulter XL). (hawaii.edu)
  • In order to be qualified for running samples with no technical assistance on the Altra flow cytometer, the user must have spent a minimum of 2 hours technician-assisted run time for training and be deemed by the facility director to be qualified to use the instrument un-assisted. (hawaii.edu)
  • The XL flow cytometer can be used with more minimal training. (hawaii.edu)
  • These systems combine the sample delivery and data output of a flow cytometer with the detection capabilities of a Time of Flight mass spectrometer. (ncl.ac.uk)
  • In this medical test, the sample is suspended in fluid which passes through a flow cytometer, a device designed to identify particles in a solution. (wisegeek.com)
  • If clinical applications are part of your requirements, the streamlined, "load and go" Aquios CL flow cytometer is designed for high precision and integrated automated sample preparation. (beckman.com)
  • Hi, Soon we will test a demo flow cytometer. (protocol-online.org)
  • Since then he has worked for Flow Cytometer manufacturers, training and supporting customers as well as launching and marketing new systems. (rms.org.uk)
  • An event is either an actual biological cell or some other mass that was large enough to trigger the data acquisition capturing device of the flow cytometer instrument. (wikipedia.org)
  • fluorescence
  • The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster , filed for patent on 18 December 1968 and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen . (wikipedia.org)
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • Impulszytophotometrie ), based on the first patent application on fluorescence-based flow cytometry. (wikipedia.org)
  • Among the measurements derived from flow cytometry are the size, relative fluorescence and complexity of the particle. (scripps.edu)
  • The cells flow single file past focused laser beams and light scatter or fluorescence data is collected. (scripps.edu)
  • He specialises in Imaging Flow Cytometry and the use of fluorescence dyes to track cell proliferation. (rms.org.uk)
  • PnB] Flow cytometry data is typically saved for analysis in the form of an array, with fluorescence and scatter channels represented in columns, and individual "events" (most of which are cells) forming the rows. (wikipedia.org)
  • laboratory
  • After studying Chemistry at Salford University, Richard worked in the oligonucleotide synthesis group at the MRC's Laboratory of Molecular Biology, before changing direction and running the flow cytometry equipment for the LMB for 8 years. (rms.org.uk)
  • markers
  • More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)
  • cell
  • Owing to its capacity to measure events at a single cell level, flow cytometry allows for the identification of rare signaling events (see example below). (cellsignal.com)
  • Helen's interest in flow cytometry began when she was a DPhil student investigating B cell tolerance in autoimmunity. (rms.org.uk)
  • A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high profile publications in journals including Science and Cell. (rms.org.uk)
  • Flow microfluorimetry is also used in pharmaceutical research to determine cell type, protein and DNA expression, cell cycle, and other properties of a cell during drug treatment. (wikipedia.org)
  • Blood counting machines aspirate a very small amount of the specimen through narrow tubing followed by an aperture and a laser flow cell. (wikipedia.org)
  • fluid
  • Flow cytometry measures phenotypic, biochemical and molecular characteristics of individual cells or particles suspended in a fluid stream. (scripps.edu)
  • particles
  • Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they move in a stream and are passed through a laser. (scripps.edu)
  • Another use of microfluorometry is flow cytometry which uses the emission of fluorochrome molecules and usually a laser as a light source to create data from particles and cells. (wikipedia.org)
  • Core
  • The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis. (scripps.edu)
  • identify
  • Alas, the task in flow cytometry is to identify a signal that is a million times weaker than the illumination light. (azooptics.com)
  • Thus flow cytometry, unlike western blotting has the capacity to identify and resolve this small but real sub-population of B lymphocytes from a heterogeneous pool of human PBMCs. (cellsignal.com)
  • laser
  • Thousands of cells per second are counted and then characterized in biotechnical and medical analytics with laser-based flow cytometry. (azooptics.com)
  • As cells pass through the flow chamber they are hit with the laser light beam which is scattered in different directions and recorded as forward light scatter and side light scatter. (novapublishers.com)