• fluorescence
  • The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster , filed for patent on 18 December 1968 and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen . (wikipedia.org)
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • Impulszytophotometrie ), based on the first patent application on fluorescence-based flow cytometry. (wikipedia.org)
  • He specialises in Imaging Flow Cytometry and the use of fluorescence dyes to track cell proliferation. (rms.org.uk)
  • PnB] Flow cytometry data is typically saved for analysis in the form of an array, with fluorescence and scatter channels represented in columns, and individual "events" (most of which are cells) forming the rows. (wikipedia.org)
  • laboratory
  • After studying Chemistry at Salford University, Richard worked in the oligonucleotide synthesis group at the MRC's Laboratory of Molecular Biology, before changing direction and running the flow cytometry equipment for the LMB for 8 years. (rms.org.uk)
  • ISLH has also expanded its focus to include a number of sub-disciplines related to laboratory hematology, including Cellular Analysis, Flow Cytometry, Hemostasis and Thrombosis, Molecular Diagnostics, Hematology Informatics, Hemoglobinopathies, Hemolytic Anemias, Point of Care Testing and Standards and guidelines. (wikipedia.org)
  • particles
  • A method of measuring the characteristics of microscopic particles, usually cells, as they flow in a fluid stream through a beam of light. (photonics.com)
  • Another use of microfluorometry is flow cytometry which uses the emission of fluorochrome molecules and usually a laser as a light source to create data from particles and cells. (wikipedia.org)
  • cell
  • Helen's interest in flow cytometry began when she was a DPhil student investigating B cell tolerance in autoimmunity. (rms.org.uk)
  • A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high profile publications in journals including Science and Cell. (rms.org.uk)
  • Flow microfluorimetry is also used in pharmaceutical research to determine cell type, protein and DNA expression, cell cycle, and other properties of a cell during drug treatment. (wikipedia.org)
  • Blood counting machines aspirate a very small amount of the specimen through narrow tubing followed by an aperture and a laser flow cell. (wikipedia.org)
  • software
  • The software developed by the researchers generates a two-parameter dot-plot display, similar to those found in flow cytometry, offering the objectivity of the latter method but with tools generally available to hematopathologists and other clinicians. (photonics.com)
  • Since then, FCS became the standard file format supported by all flow cytometry software and hardware vendors. (wikipedia.org)
  • markers
  • More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)
  • method
  • As reported in the Jan. 15 issue of Cytometry Part B , the method converts immunohistochemistry data to a two-parameter dot-plot display, much like those found in flow cytometry. (photonics.com)
  • Thus it provides a sort of "virtual" flow cytometry, offering the objectivity of the latter method but with relatively inexpensive tools that are readily available to most hematopathologists. (photonics.com)
  • Researchers have developed a method for converting immunohistochemistry data into objective, flow cytometrylike information. (photonics.com)
  • device
  • The first impedance-based flow cytometry device, using the Coulter principle , was disclosed in U.S. Patent 2,656,508, issued in 1953, to Wallace H. Coulter . (wikipedia.org)
  • cells
  • Thousands of cells per second are counted and then characterized in biotechnical and medical analytics with laser-based flow cytometry. (azooptics.com)
  • In this step, the cells attached to the nanoparticles (expressing the antigen) stay on the column, while other cells (not expressing the antigen) flow through. (wikipedia.org)