• Chromosomes
  • Joe Hin Tjio working in Albert Levan's lab was responsible for finding the approach: Using cells in culture Pre-treating cells in a hypotonic solution, which swells them and spreads the chromosomes Arresting mitosis in metaphase by a solution of colchicine Squashing the preparation on the slide forcing the chromosomes into a single plane Cutting up a photomicrograph and arranging the result into an indisputable karyogram. (wikipedia.org)
  • During the 1990s he realized together with P. Lichter the concept of comparative genomic hybridization to metaphase chromosomes and to a matrix with DNA spots representing specific genomic sites. (wikipedia.org)
  • genes
  • Our findings indicate that aberrant methylation seems to be a mechanism for NF2 gene inactivation, considered an early step in schwannoma tumorigenesis, and as well, aberrant hypermethylation of other tumor-related genes might represent secondary events that also contribute to the development of these tumors. (aacrjournals.org)
  • Sequencing of several candidate genes within the mapped interval led to negative findings in terms of the underlying molecular pathogenesis of the disease in the family. (csic.es)
  • Exome sequencing, also known as whole exome sequencing (WES), is a genomic technique for sequencing all of the protein-coding genes in a genome (known as the exome). (wikipedia.org)
  • locus
  • Northern hybridization experiments confirmed that the predicted smr35B locus did express a single transcript of the expected size, which accumulated differentially in free-living and endosymbiotic bacteria. (wikipedia.org)
  • clinical
  • This technique was popularized in genomics as a means of finding clusters of patients with similar gene expression patterns with a view to finding subcategories of tumors with distinct clinical characteristics. (aacrjournals.org)
  • results
  • To evaluate the prognostic significance of cytogenetic findings in clear cell renal cell carcinoma (RCC), cytogenetic results of 118 primary RCCs were evaluated in relation to classical indicators of prognosis and overall survival. (aacrjournals.org)
  • This CM was used in a further search for new members of the αr9 family in the existing bacterial genomic databases and resumed in the following Table 2: The results were manually inspected to deduce a consensus secondary structure for the family (Figure 1 and Figure 2). (wikipedia.org)
  • method
  • Several target-enrichment strategies have been developed since the original description of the direct genomic selection (DGS) method in 2005. (wikipedia.org)
  • This method was developed to improve on the hybridization capture target-enrichment method. (wikipedia.org)
  • cell
  • Loss of p53 function in urothelial cell carcinoma (UCC) by mutation or inactivation disrupts normal cell cycle checkpoints, generating a favorable milieu for genomic instability, a hallmark of UCC. (aacrjournals.org)
  • family
  • This CM was used in a further search for new members of the αr35 family in the existing bacterial genomic databases. (wikipedia.org)
  • Value
  • The value of comparative genomic hybridisation (CGH) to differentiate between metastasis and second primary, or to identify the primary location in cases of metastases and two primary tumours was studied in seven patients. (bmj.com)
  • rare
  • Since DECIPHER is opened to any accredited clinician or cytogeneticist from around the world, the chances of finding similar rare cases are significantly increased. (wikipedia.org)