• Chromosomes
  • Joe Hin Tjio working in Albert Levan's lab was responsible for finding the approach: Using cells in culture Pre-treating cells in a hypotonic solution, which swells them and spreads the chromosomes Arresting mitosis in metaphase by a solution of colchicine Squashing the preparation on the slide forcing the chromosomes into a single plane Cutting up a photomicrograph and arranging the result into an indisputable karyogram. (wikipedia.org)
  • During the 1990s he realized together with P. Lichter the concept of comparative genomic hybridization to metaphase chromosomes and to a matrix with DNA spots representing specific genomic sites. (wikipedia.org)
  • genome-wide
  • Using genome‐wide tests increases the likelihood that findings outside the target area will be made. (els.net)
  • Major research efforts in the field include sequence alignment, gene finding, genome assembly, drug design, drug discovery, protein structure alignment, protein structure prediction, prediction of gene expression and protein-protein interactions, genome-wide association studies, the modeling of evolution and cell division/mitosis. (wikipedia.org)
  • locus
  • Northern hybridization experiments confirmed that the predicted smr35B locus did express a single transcript of the expected size, which accumulated differentially in free-living and endosymbiotic bacteria. (wikipedia.org)
  • clinical
  • Whole‐genome approaches, which are replacing targeted tests in research and clinical practice, increase the chances of 'incidental findings' (IFs) - that is, those unrelated to the reason for the test. (els.net)
  • platforms
  • Despite the diversity of platforms, ultimately they all use genomic DNA from disrupted cells to recreate a high resolution karyotype in silico. (wikipedia.org)
  • results
  • To evaluate the prognostic significance of cytogenetic findings in clear cell renal cell carcinoma (RCC), cytogenetic results of 118 primary RCCs were evaluated in relation to classical indicators of prognosis and overall survival. (aacrjournals.org)
  • This CM was used in a further search for new members of the αr9 family in the existing bacterial genomic databases and resumed in the following Table 2: The results were manually inspected to deduce a consensus secondary structure for the family (Figure 1 and Figure 2). (wikipedia.org)
  • However
  • However, research funding for such individualised approaches is often insufficient: protocols do not always include quality assurance procedures, and research teams lack health professionals who can communicate findings to participants. (els.net)
  • data
  • Early empirical data show that the chances of finding IFs range between 1% and 7%, depending on the test used. (els.net)
  • We also explain how to construct the problem in a more appropriate, hypothesis testing framework, and provide comparative analyses of data from patients with lung cancer to illustrate the concepts. (aacrjournals.org)
  • research
  • Researchers generally are not thought to have a duty of care to research participants, but some experts argue that they ought to return IFs that are clinically valid, medically important and actionable, or even 'hunt' for additional findings that fit these criteria. (els.net)
  • 2014) Informed consent for return of incidental findings in genomic research. (els.net)
  • This finding led Thomas Cremer to his concept of a new field of cytogenetic research, called by him as interphase cytogenetics. (wikipedia.org)
  • family
  • This CM was used in a further search for new members of the αr35 family in the existing bacterial genomic databases. (wikipedia.org)
  • patients
  • The value of comparative genomic hybridisation (CGH) to differentiate between metastasis and second primary, or to identify the primary location in cases of metastases and two primary tumours was studied in seven patients. (bmj.com)
  • target
  • Several target-enrichment strategies have been developed since the original description of the direct genomic selection (DGS) method in 2005. (wikipedia.org)
  • This method was developed to improve on the hybridization capture target-enrichment method. (wikipedia.org)