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  • fluorescence in situ hybr
  • We compared specificity and sensitivity of the chromosome CGH and aCGH techniques by comparing array and chromosome CGH in the same tumor samples, by counting fluorescence in situ hybridization (FISH) signals generated by BAC clones used in the array, and by using real-time quantitative PCR. (aacrjournals.org)
  • Physical Symptoms Heart Defects Characteristics of Autism Genital defects (in males) Childhood hypotonia Respiratory infections Motor Delay Renal defects Behavioural Symptoms Passiveness Sociability Aggression Biting, and/or hitting Moodiness Disliking routine changes Tests are either conducted at birth, or later in early childhood via: fluorescence in situ hybridization (FISH), multiplex ligation-dependent probe amplification (MLPA), array comparative genomic hybridization (aCGH), and EHMT1 sequencing. (wikipedia.org)
  • Chromosomes
  • Joe Hin Tjio working in Albert Levan's lab was responsible for finding the approach: Using cells in culture Pre-treating cells in a hypotonic solution, which swells them and spreads the chromosomes Arresting mitosis in metaphase by a solution of colchicine Squashing the preparation on the slide forcing the chromosomes into a single plane Cutting up a photomicrograph and arranging the result into an indisputable karyogram. (wikipedia.org)
  • During the 1990s he realized together with P. Lichter the concept of comparative genomic hybridization to metaphase chromosomes and to a matrix with DNA spots representing specific genomic sites. (wikipedia.org)
  • probes
  • To capture genomic regions of interest using in-solution capture, a pool of custom oligonucleotides (probes) is synthesized and hybridized in solution to a fragmented genomic DNA sample. (wikipedia.org)
  • The probes (labeled with beads) selectively hybridize to the genomic regions of interest after which the beads (now including the DNA fragments of interest) can be pulled down and washed to clear excess material. (wikipedia.org)
  • microarray
  • 2014) A new direction for prenatal chromosome microarray testing: software‐targeting for detection of clinically significant chromosome imbalance without equivocal findings. (els.net)
  • locus
  • Northern hybridization experiments confirmed that the predicted smr35B locus did express a single transcript of the expected size, which accumulated differentially in free-living and endosymbiotic bacteria. (wikipedia.org)
  • data
  • Early empirical data show that the chances of finding IFs range between 1% and 7%, depending on the test used. (els.net)
  • results
  • To evaluate the prognostic significance of cytogenetic findings in clear cell renal cell carcinoma (RCC), cytogenetic results of 118 primary RCCs were evaluated in relation to classical indicators of prognosis and overall survival. (aacrjournals.org)
  • This CM was used in a further search for new members of the αr9 family in the existing bacterial genomic databases and resumed in the following Table 2: The results were manually inspected to deduce a consensus secondary structure for the family (Figure 1 and Figure 2). (wikipedia.org)
  • However
  • However, research funding for such individualised approaches is often insufficient: protocols do not always include quality assurance procedures, and research teams lack health professionals who can communicate findings to participants. (els.net)
  • research
  • Researchers generally are not thought to have a duty of care to research participants, but some experts argue that they ought to return IFs that are clinically valid, medically important and actionable, or even 'hunt' for additional findings that fit these criteria. (els.net)
  • 2014) Informed consent for return of incidental findings in genomic research. (els.net)
  • This finding led Thomas Cremer to his concept of a new field of cytogenetic research, called by him as interphase cytogenetics. (wikipedia.org)
  • method
  • Several target-enrichment strategies have been developed since the original description of the direct genomic selection (DGS) method in 2005. (wikipedia.org)
  • This method was developed to improve on the hybridization capture target-enrichment method. (wikipedia.org)