Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). (cellsignal.com)
Flow cytometric analysis of Hela cells using β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 647 Conjugate) #2985 (dashed line). (cellsignal.com)
Western blot analysis of extracts from various cell lines using β-Actin (13E5) Rabbit mAb (Biotinylated). (cellsignal.jp)
Proteins4
Actin is a family of globular multi-functional proteins that form microfilaments in the cytoskeleton, and the thin filaments in muscle fibrils. (wikipedia.org)
The evolutionary origin of actin can be traced to prokaryotic cells, which have equivalent proteins. (wikipedia.org)
A large number of illnesses and diseases are caused by mutations in alleles of the genes that regulate the production of actin or of its associated proteins. (wikipedia.org)
Third, actin filaments can bind to many other proteins, which together help modify and organize microfilaments for their diverse functions. (wikipedia.org)
Interactions of actin2
Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes. (wikipedia.org)
Tropomyosin is a two-chain coiled coil protein, which together with the troponin complex controls interactions of actin with myosin in a Ca 2+ -dependent manner. (nih.gov)
Isoforms2
In fast skeletal muscle, the contractile actin filaments are regulated by tropomyosin isoforms Tpm1.1 and Tpm2.2, which form homo- and heterodimers. (nih.gov)
5. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. (nih.gov)
Monoclonal Antibody1
Monoclonal antibody is produced by immunizing rabbit with full length human β-actin and purified using protein A resin. (lifetein.com)
Globular3
Actin is a family of globular multi-functional proteins that form microfilaments in the cytoskeleton, and the thin filaments in muscle fibrils. (wikipedia.org)
It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. (wikipedia.org)
β-actin exists in two forms in cytoplasm: globular actin monomer (G-actin) with molecular weight approximately 42 kDa and fibrous polymer (F-actin). (lifetein.com)
Filaments6
An actin protein is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. (wikipedia.org)
It is believed that the diverse range of structures formed by actin enabling it to fulfill such a large range of functions is regulated through the binding of tropomyosin along the filaments. (wikipedia.org)
Actin homologs from prokaryotes and archaea polymerize into different helical or linear filaments consisting of one or multiple strands. (wikipedia.org)
The multifaceted role of actin relies on a few of the microfilaments' properties: First, the formation of actin filaments is reversible, and their function often involves undergoing rapid polymerization and depolymerization. (wikipedia.org)
Third, actin filaments can bind to many other proteins, which together help modify and organize microfilaments for their diverse functions. (wikipedia.org)
In most cells actin filaments form larger-scale networks which are essential for many key functions: Actin networks give mechanical support to cells and provide trafficking routes through the cytoplasm to aid signal transduction. (wikipedia.org)
Malignant3
Aims: We compared the clinical and pathological features of pleomorphic malignant fibrous histiocytoma (MFH)-like soft tissue sarcomas with and without myofibroblastic differentiation on electron microscopy. (johnshopkins.edu)
6. Expression of smooth muscle markers in so called malignant fibrous histiocytomas. (nih.gov)
11. Actin expression in neural crest cell-derived tumors including schwannomas, malignant peripheral nerve sheath tumors, neurofibromas and melanocytic tumors. (nih.gov)
Myosin1
2. Differential expression of smooth muscle myosin, smooth muscle actin, h-caldesmon, and calponin in the diagnosis of myofibroblastic and smooth muscle lesions of skin and soft tissue. (nih.gov)
Eukaryotic3
Due to its ubiquitous expression in eukaryotic cells, β-actin is widely used as a loading control for western blotting, markers for the integrity of cells. (lifetein.com)
Second, homologues of signature eukaryotic proteins, such as actin and tubulin that form the core of the cytoskeleton or the ubiquitin system, have been detected in diverse archaea. (catsboard.com)
The actin and tubulin cytoskeletons, the nuclear pore, the spliceosome, the proteasome and the ubiquitin signalling system are only a few of the striking examples of the organizational complexity that seems to be a 'birthright' of eukaryotic cells. (catsboard.com)
In seven myofibroblastic cases, smooth muscle in four cases and muscle-specific actin in two cases, desmin in three cases and S100 in one case were present. (johnshopkins.edu)
Mutations3
A large number of illnesses and diseases are caused by mutations in alleles of the genes that regulate the production of actin or of its associated proteins. (wikipedia.org)
Mutations in the different genes that regulate actin production in humans can cause muscular diseases, variations in the size and function of the heart as well as deafness. (wikipedia.org)
Both mutations tended to decrease actin affinity of homo-and heterodimers in the absence and presence of troponin and Ca 2+ , although the effect of Q93H was stronger. (nih.gov)
Tumours1
9. Alpha-smooth muscle actin as a marker for soft tissue tumours: a comparison with desmin. (nih.gov)
Soft tissue1
10. Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors. (nih.gov)
Contractile1
The alpha actins, found in muscle tissues, are a major constituent of the contractile apparatus. (wikipedia.org)
Rabbit4
Confocal immunofluorescent analysis of HeLa cells using Pan-Actin (D18C11) Rabbit mAb (green). (cellsignal.com)
Western blot analysis of extracts from various cell lines using Pan-Actin (D18C11) Rabbit mAb. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Pan-Actin (D18C11) Rabbit mAb. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded human heart using Pan-Actin (D18C11) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). (cellsignal.com)
Cell5
Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. (wikipedia.org)
The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. (wikipedia.org)
Rapid assembly and disassembly of actin network enables cells to migrate (Cell migration). (wikipedia.org)
Its location is regulated by cell membranesignal transduction pathways that integrate the stimuli that a cell receives stimulating the restructuring of the actin networks in response. (wikipedia.org)
β-actin is a member of highly conserved actins that are involved in cell motility, structure, and integrity. (lifetein.com)
Muscle1
Actin is extremely abundant in most cells, comprising 1-5% of the total protein mass of most cells, and 10% of muscle cells. (wikipedia.org)
Expression1
Lastly, actin plays an important role in the control of gene expression. (wikipedia.org)