Fibroblasts cell culture techniques. Medical search. Frequent questions

In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). (elsevierpure.com)
For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. (elsevierpure.com)
The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. (elsevierpure.com)
Here, by using a high-throughput small-molecule microarray-based screening of cGAS interacting compounds, we identify brivanib, known as a dual inhibitor of vascular endothelial growth factor receptor and fibroblast growth factor receptor, as a cGAS modulator. (bvsalud.org)
In immunofluorescence and electron microscopy of cultured fibroblasts, as well as of various other cells (endothelial, epithelial, lenticular, monocytes, neuroblastoma cells) and tissues (human kidney and liver), p230 was localized as a distinct subplasmalemmal layer in the peripheral cytoplasm of the cells. (rupress.org)
Adherent cells such as fibroblasts, epithelial cells, and endothelial cells need a proper surface to attach to, and improper attachment can lead to cell death. (coleparmer.com)
In this project, primary fibroblasts and endothelial cells from mouse heart will be cultured and treated with doxorubicin. (uidaho.edu)
The conditioned medium was collected and assayed for VEGF or used to assess its ability to stimulate angiogenesis by measuring the proliferation of human dermal microvascular endothelial cells. (uea.ac.uk)

Keratinization is the differentiation of keratinocytes in the granular layer into dead surface cells to form the stratum corneum. (wikipedia.org)
The first occurs by migration and proliferation of keratinocytes from the wound edges and by differentiation of stem cells from remaining hair follicle bulbs. (worldwidewounds.com)
At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
Cell fusion and differentiation in the culture. (pmf.hr)
Gremlin1 contributes to the inhibitory activity of GFCM Owing to the high expression in gingival fibroblasts, we investigated the effect of depleting Gremlin1 from GFCM on osteoblastic differentiation by immunoprecipitation (IP). (bso14.org)
4.?Debate The full total outcomes of the research present that gingival fibroblasts secrete BMP antagonists, most Gremlin1 abundantly, resulting in in vitro reduced amount of ALP activity, a marker of osteoblastic differentiation in both principal calvarial osteoblasts and a rat osteosarcoma cell series. (bso14.org)
In vitro, BMPs are powerful differentiation factors, causing the differentiation of multipotential mesenchymal cells into osteochondrogenic lineage cells and osteoblast precursor cells.5, 16, 17 BMPs are portrayed during alveolar bone tissue regeneration.18, 19 Certainly, the addition of BMP2 can be able to enhance the outcomes of guided tissues regeneration in individual sufferers significantly,20 but require good sized supraphysiological doses. (bso14.org)
Disease models for GERD, BE, GVHD, and ulcerative colitis based on three-dimensional human cell and tissue culture systems that recapitulate in vivo growth and differentiation in inflammatory-associated microphysiological environments would enhance our understanding of disease progression and improve our ability to test for disease-prevention strategies. (biomedcentral.com)
These intradermal epithelial structures, such as sebaceous glands, sweat glands, and hair follicles, are lined with epithelial cells with the potential for division and differentiation. (medscape.com)
In recent years reprogramming techniques, differentiation protocols, and sequencing analysis have opened a field for generating and studying human-derived neurons with greater potential than ever before. (lu.se)
Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)

Our protocol allows researchers to generate patient-specific iTS cells to interrogate the trophoblast and placenta biology as well as their interactions with embryonic cells in health and diseases. (edu.au)

This decellularization method has been used in a variety of contexts including primary human dermal and lung fibroblast cultures, cancer-associated fibroblast cultures from esophageal, ovarian, renal, pancreatic, prostatic and lung tumors, and murine mammary fibroblasts 10,12,13 . (researchsquare.com)
They also added cancer-associated fibroblast cells that were grown in the lab of Diane Simeone, surgical director at the Multidisciplinary Pancreatic Cancer Clinic of the U-M Cancer Center. (rdworldonline.com)

Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
Cell culture technique can be performed with the help of chick embryo fibroblast cells, human amnion cells, etc. (biologyease.com)

Here, we describe the StemCellFactory, an automated, modular platform covering the entire process of hiPSC production, ranging from adult human fibroblast expansion, Sendai virus-based reprogramming to automated isolation, and parallel expansion of hiPSC clones. (frontiersin.org)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
Cell culture technique is used for isolation of viruses from the clinical specimens and for diagnosis of viral diseases. (biologyease.com)
The Chick embryo technique is the first technique used for the isolation of viruses. (biologyease.com)
The techniques that will be used in the project include cardio fibroblast isolation, cell culture, protein purification, PCR, western blotting, and mass spectrometry-based proteomics analysis. (uidaho.edu)
Viral pneumonia can be diagnosed by isolation and identification of the pathogen through viral culture. (medscape.com)
in conclusion, our proposed model proved itself efficient for keloid fibroblast isolation from specific regions and cultivation. (unifesp.br)
For HSV isolation, either tube or shell vial culture may be used. (medscape.com)

Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
This vector efficiently produced Tet-ON regulatable immortalized (293T) and primary (human mesenchymal stem cells and human primary fibroblasts) cells. (nih.gov)
B. Recombinant TNF-α enhances the mRNA expression of tenascin-C also in human primary fibroblasts. (bioz.com)
C. An immunoblot showing enhanced expression of protein for tenascin-C (M r 290,000 band) in the primary fibroblasts (the first two lanes) and in the cell culture supernatant (the latter two lanes) after stimulation with TNF-α and IFN-γ for 18 h. (bioz.com)
Decellularization allows for the generation of cell and tissue type-specific three-dimensional (3D) cell-derived matrices (CDMs) synthesized and organized by cultured primary cells. (researchsquare.com)
To this end, primary fibroblasts were seeded at a high density (25,000 cells per cm 2 ) and cultured for 7 days, as originally described for mouse NIH-3T3 cells, to generate 3D cultures 11 . (researchsquare.com)
Primary cell culture. (pmf.hr)
2. Primary cell culture: preparation of fibroblast culture from mouse skin. (pmf.hr)
What are the challenges of culturing primary cells compared to established cell lines? (coleparmer.com)
Culturing primary cells presents unique challenges compared to established cell lines. (coleparmer.com)
Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)
Primary cancer cells don't grow well on a flat surface, and like people, they need neighbors to really prosper," Nagrath said. (rdworldonline.com)
In the primary experiments, we verified that Compact disc14+ macrophage-like cells, attained by a parting technique using magnetic beads, acquired. (mingsheng88.org)
Standard human primary fibroblast and regular human bronchial epithelia were bought from Cambrex and grown within their extraordinary culture media as per instructions of your vendor. (micrornaarray.com)
We suggest an explant model for keloid fibroblasts harvesting, standardising the initial processing of keloid samples to obtain fragments from different regions, considering its biological differences, for primary cell culture. (unifesp.br)
Primary fibroblast culture was carried out by explant. (unifesp.br)
We also use primary retinal cells to investigate the effects of nanowires on different cell types, in particular neurons compared to glial cells. (lu.se)

Here we describe a step-by-step protocol for the generation and establishment of human iTS cells directly from dermal fibroblasts using a non-integrative reprogramming method. (edu.au)
The protocol consists of four main sections: (1) recovery of cryopreserved human dermal fibroblasts, (2) somatic cell reprogramming, (3) passaging of reprogramming intermediates and (4) derivation of iTS cell cultures followed by routine maintenance of iTS cells. (edu.au)
B lymphocytes and B-cell activating factor promote collagen and profibrotic markers expression by dermal fibroblasts in systemic sclerosis. (ox.ac.uk)
However, the ability of B cells directly to stimulate dermal fibroblasts and the role of BAFF are not fully understood. (ox.ac.uk)
We therefore investigated the involvement of B cells and BAFF in the expression of collagen and profibrotic markers by dermal fibroblasts. (ox.ac.uk)
METHODS: Cocultures of blood B cells from healthy blood donors and normal or SSc dermal fibroblasts stimulated with anti-IgM and BAFF were performed. (ox.ac.uk)
RESULTS: Coculture of blood B cells and dermal fibroblasts isolated from SSc patients induced IL-6, TGF-β1, CCL2, and collagen secretion, as well as Alpha-SMA, TIMP1, and MMP9 expression in dermal fibroblasts. (ox.ac.uk)
CONCLUSIONS: Our results showed for the first time a direct role of B cells on the production of collagen by dermal fibroblasts, which is further enhanced by BAFF. (ox.ac.uk)

Tissue engineering of oral mucosa combines cells, materials and engineering to produce a three-dimensional reconstruction of oral mucosa. (wikipedia.org)
Partial-thickness tissue engineering uses one type of cell layer, this can be in monolayers or multilayers. (wikipedia.org)
Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
For example, despite improvements in cell culture techniques and developments in dermal matrices, tissue-engineered skin substitutes have yet to achieve widespread use by clinicians. (worldwidewounds.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
We first asked whether a cell-autonomous mechanism could contribute to exhaled biomarkers, so we began by searching for thematic differences in metabolic gene expression between EAC and relevant control tissue in archived transcriptomic datasets. (nature.com)
Tissue and cell type-specific cell-free extracellular matrices generated by various decellularization protocols can be important tools to study cell interactions with their distinct extracellular niche. (researchsquare.com)
The protocols for decellularization are dependent on cell and tissue type and species of origin usually requiring multiple chemical and biological reagents supplied in a series of incubations 6 . (researchsquare.com)
Sterile glasswares, pre-sterilized tissue culture, plastic wares. (biologyease.com)
For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)
And because the cells circulate in the blood, they can be gathered with a blood draw rather than a more invasive tissue biopsy. (rdworldonline.com)
The technique may also bring doctors closer to their goal of capturing cancer cells for diagnosis with a quick, non-invasive "blood biopsy" instead of the tissue biopsies that are currently used. (rdworldonline.com)
Respiratory secretions, bronchoalveolar lavage samples, and tissue specimens can be examined using cytologic and histologic techniques. (medscape.com)
Tissue from the upper or lower respiratory tract, sputum samples, and samples obtained by nasopharyngeal washing, bronchoalveolar lavage, and biopsy may be submitted for viral culture. (medscape.com)
Infiltrating immune cells and stromal components of tissue including fibroblasts contribute to dynamic changes occurring in tissue related to disease development. (biomedcentral.com)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
A great many noxious agents capable of inducing SCE in tissue culture cells are carcinogenic (see, for example, Wolff, 1977 ). (biologists.com)
These conditionally reprogrammed epithelial cells indicated markers of adult stem cells, but not of embryonic and induced pluripotent stem cells, and appeared to consist of cell populations resembling their main tissue of source, making them ideal to study tissue regeneration, as well as normal physiologic and pathologic processes [8]. (cell-metabolism.com)
Normal multipotent tissue stem cells (SCs) are the driving force behind tissue turnover and repair. (medscape.com)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
In this project, involving engineers from LTH, we apply advanced microfluidic techniques to culture hESCs under the influence of chemical gradients to mimic the environment around the developing brain in the fetus, thereby generating neural tissue with anatomical characteristics resembling the developing human brain. (lu.se)

The second occurs by influx of growth factors secreted by macrophages, platelets and fibroblasts, by fibroblast proliferation and subsequent synthesis and remodelling of collagenous dermal matrix. (worldwidewounds.com)
The effect of the materials on the cultivated fibroblasts was analyzed in terms of short-term and long-term cell growth as well as in viability and cell proliferation assays. (bvsalud.org)
Immune cells can potentiate oxidative stress, and fibroblasts have the capacity to contribute to advanced growth and proliferation of the epithelium and any resultant cancers. (biomedcentral.com)
We investigate immortalized cells in terms of migration, proliferation, stress and membrane conformation on the nanowires. (lu.se)

Together, this suggests the esophagus is exposed to aldehyde stress, and that glandular esophageal cells may be vulnerable to this stress. (nature.com)

In vitro results showed naringin presented cytotoxic effects, reduced the number of colonies, inhibited cell migration, and changed the morphology and cell cycle progression of the two cell lines evaluated. (bvsalud.org)
KetacMolar Easymix (3M/ESPE) was the glass ionomer cement with the least amount of cytotoxic activity on fibroblasts. (bvsalud.org)
On this study we observed that forced in excess of expression of bcl xL protein desensitized T cells towards the effects of specified cytotoxic chemotherapeutic agents. (micrornaarray.com)
These experiments imply a potentially novel way for you to have an effect on the resistance of bladder carcinoma cells to cytotoxic chemotherapy. (micrornaarray.com)
Inflammation initiated by an immune response targets epithelial cells, leading to cytotoxic effects, and is a key driver of several disease conditions in the gastrointestinal (GI) tract [ 1 ]. (biomedcentral.com)
The cytotoxic potential of 0.4% RPE in oral fibroblasts was observed after 1, 3 and 5 min of contact. (bvsalud.org)

Multi-walled carbon nanotube (MWCNT)-induced fibrogenic signaling in a human lung epithelial-fibroblast co-culture system. (cdc.gov)
In this study, we assessed the effects of pristine MWCNT at an occupationally relevant dose on fibroblasts grown in a transwell co-culture system of apical Beas-2B human bronchial epithelial cells and basolateral WI-38 normal human lung fibroblasts. (cdc.gov)
A. Recombinant TNF-α increases the mRNA expression of tenascin-C in foetal lung fibroblasts (MRC-9). (bioz.com)
Recently, this co-culture technique was altered to include epithelial cells from prostate, buy Dihydrotanshinone I breast, trachea, liver and lung, wherein the use of J2 cells and the Rho kinase inhibitor Y-27632 (Y) resulted in the quick reprogramming of cells into immortalized karyotype-stable ethnicities [6, 7]. (cell-metabolism.com)

We find that fibroblast-derived TNF-α stimulates epithelial LOX expression through activation of nuclear factor κB and TGF-β-mediated signaling. (nih.gov)
Bioactive glass stimulates fibroblasts to secrete significantly increased amounts of angiogenic growth factors and therefore has a number of potential applications in therapeutic angiogenesis. (uea.ac.uk)

In further support, MWCNT-exposed WI-38 in co-culture exhibit increased collagen type I, a hallmark of fibrosis, as compared to unexposed WI-38 in co-culture. (cdc.gov)
B cell- and BAFF-induced collagen secretion was highly reduced by anti-TGF-β1 antibodies. (ox.ac.uk)
After the cancer cells were captured on the chip, the team pumped in a mixture of collagen and Matrigel growth medium. (rdworldonline.com)
The collagen and Matrigel provide a three-dimensional environment for the cells to grow, while the cancer-associated fibroblasts give them the neighboring cells they need. (rdworldonline.com)

In a recent study, we showed that human fibroblasts can be reprogrammed into induced trophoblast stem (iTS) cells by transcription factor-mediated nuclear reprogramming using the Yamanaka factors OCT4, KLF4, SOX2 and c-MYC (OKSM) and a selection of TS cell culture conditions. (edu.au)
While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
Placental stem cells. (scielo.org.ar)
Empowered with PhytoCellTec ™ Malus Domestica which to protect and to repair the skin's natural stem cells and associated functionalities. (blogspot.com)
Thus the aging process is able to slow down by optimising the skin's stem cells performance. (blogspot.com)
Stem cells of an old apple cultivar with very good storage properties were produced by applying plant cell culture technique. (blogspot.com)
a naïve LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). (mdc-berlin.de)
We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naïve ES-like/ICM properties. (mdc-berlin.de)
The CAM method is a two-stage LTBMC assessing the potential of hematopoietic stem cells. (bremel.gr)
In this project we use induced pluripotent stem cell lines from various primate species to perform direct comparative analysis in stem cell derived cultures such as cerebral organoids. (lu.se)
We also use human induced pluripotent stem cells to investigate how polymorphic transposable elements contribute a genetic component to these disorders, using X-linked dystonia Parkinsonism as our current focus. (lu.se)
This is a tool to help students find the right project for their Bachelor's and Master's thesis at Lund Stem Cell Center. (lu.se)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)
Cancer stem cell-associated proteins revealed by proteomics. (medscape.com)
However, with a growing number of novel cell sources (stem cells and reprogrammed cells) it becomes important to develop new methodologies for a more thorough assessment of human neurons generated using these approaches. (lu.se)
The cells are called induced neurons (iNs) and offer a shortcut over induced pluripotent stem cells (iPSc) for generating patient and disease-specific neurons. (lu.se)
definitive treatment is hematopoietic stem cell transplantation. (msdmanuals.com)

To address the impact of cell-to-cell communication between the epithelium and underlying fibroblasts on pulmonary fibrosis, we first measured chemokine (C-C motif) ligand 2 (CCL2) concentrations by ELISA in monoculture and co-culture conditioned media. (cdc.gov)
If fibroblasts are absent from the matrix, the epithelium stops proliferating but continues to differentiate. (wikipedia.org)
Cell culture of 5637 human cell line (cancerous bladder epithelium). (bremel.gr)
Epidermal appendages are important sources of epithelial cells that re-epithelialize when the overlying epithelium is removed or destroyed in patients with partial thickness burns , abrasions, or split-thickness skin graft harvesting. (medscape.com)

In vitro studies of fibroblasts in monoculture have also investigated MWCNT toxicity. (cdc.gov)
In vitro studies with keloid fibroblasts frequently present contradictory results. (unifesp.br)

Considering the biological closeness between EGF and FGF-2, results indicate the possibility of a novel isoform of these growth factors secreted by amniotic epithelial cells. (scielo.org.ar)

However, the role of other cell types and cellular communication in the mechanisms of MWCNT-induced fibrosis needs further study. (cdc.gov)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
It constituted less than 0.3% of the total cellular protein in cultured fibroblasts and was not extractable with Triton X-100. (rupress.org)
We measure cellular forces in cells and tissues with high spatial resolution using arrays of vertical nanowires. (lu.se)

The growth of the viruses in the cell line can be detected by changes in the morphology of infected cells. (biologyease.com)
Morphology - colony formation - staining - aging - physiological comparison, with acute leukemia marrow fat cells. (bremel.gr)

Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)

At the time of their establishment, 3T3 cells were different than most other cell lines in regard to the fact they did not induce tumors to develop when injected into murine species. (fsu.edu)

Cell biology techniques: Immunostaining, western blot. (ibecbarcelona.eu)

Figure 1: Comparison of analyzers with respect to their ease of implementation in microbial fermentations and mammalian cell culture unit operations for various quality attributes: (a) misincorporation, (b) nutrients, (c) glycosylation, and (d) cell growth. (biopharminternational.com)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

Below SYPRO Ruby staining of the immunoblot confirming equal loading and transfer of proteins from cell lysate samples. (bioz.com)
Our recent findings have shown that the widely used NH 4 OH/Triton X-100/DNase decellularization method is not able to completely remove cytoskeletal proteins from long-term three-dimensional human gingival fibroblast cultures. (researchsquare.com)
This protocol efficiently removes cytoskeletal proteins, including actin and beta-tubulin, and DNA from the cultures, which could interfere with the cell-matrix interactions of cells seeded on the decellularized matrices. (researchsquare.com)

Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
Lastly, detaching adherent cells from the surface for passaging or analysis can be tough and can lead to cell damage. (coleparmer.com)
Spontaneous appearance of IL-11 mRNA in clean RSC We following analyzed the gene appearance of IL-11 in clean synovial adherent cells extracted from RA and osteoarthritis (OA). (mingsheng88.org)
In contrast to soluble chemicals, cadmium oxide particles on a cm2/ml media basis, but are only ~50 particles can settle, diffuse, and aggregate differentially accord- times more potent when differences in delivery to adherent cells are ing to their size, density, and surface physicochemistry. (cdc.gov)

and fibroblast-epithelial cell coculture, the latter coupled with fluorescence-activated cell sorting. (nih.gov)

Metalloproteinases (NIMP) produced by both cancer and normal stromal fibroblast cells play a critical role in the metastatic spread of tumours, however little is known of the regulation of their release. (birmingham.ac.uk)

Contact inhibited, a confluent monolayer of 3T3 cells yields approximately 40,000 cells per square centimeter. (fsu.edu)
Human T and bladder carcinoma cell lines have been routinely cultured in carbon dioxide ambiance and passaged when to confluent. (micrornaarray.com)

A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Results Co\culture experiments showed GF\secreted factors that inhibit BMP\stimulated ALP activity. (bso14.org)
These experiments are closely linked to epigenetic analysis and gene editing experiments performed in cell culture models of glioma. (lu.se)

Using gene manifestation profiling, practical siRNA library testing and reverse-phase protein arrays (RPPA), we buy Dihydrotanshinone I found that J2 cells and Y produced a cooperative effect on human being foreskin keratinocytes (HFKs), which resulted in suppression of TGF- signaling juxtaposed with up-regulation of changes in cell cycle progression, cell adhesion and motility. (cell-metabolism.com)
Materials and Methods Cells Human being keratinocytes were cultured from discarded and de-identified neonatal foreskin cells. (cell-metabolism.com)
Cultured eukaryote cells such as human foreskin fibroblasts or vero cell cultures are used to inoculate the specimen. (medscape.com)

Transwell assays demonstrated that this induction was dependent on cell-cell contact. (ox.ac.uk)

Oral mucosa, however, is a complex multilayer structure with proliferating and differentiating cells and monolayer epithelial sheets have been shown to be fragile, difficult to handle and likely to contract without a supporting extracellular matrix. (wikipedia.org)
Monolayer epithelial sheets can be used to manufacture multilayer cultures. (wikipedia.org)
Now a day's monolayer cell cultures are most widely used in diagnostic and research purposes in viral diseases. (biologyease.com)
Form the monolayer of cell culture in a sterile flask which is dispersed with the treatment of trypsin and versene. (biologyease.com)
Add a small amount of MEM and 10% FCS to the monolayer of the cell. (biologyease.com)
Count the number of monolayer cells in a haemocytometer. (biologyease.com)
Observe the cytopathic changes in the monolayer of cells during 7 days of incubation. (biologyease.com)
After incubation, examine the flask of the healthy monolayer of cells and as well as infected cells. (biologyease.com)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
Collected samples should be layered on a monolayer of cells in test tubes. (medscape.com)

Researchers say it's a major step forward in the study of circulating tumor cells, which are shed from tumors and circulate through the blood of cancer patients. (rdworldonline.com)

Amniotic epithelial cells were believed to secrete an 'Epidermal Growth Factor (EGF) - like' factor and exact identification was not made. (scielo.org.ar)
The aim of this study was to assess whether it is possible to encapsulate specific quantities of bioactive glass and fibroblasts into alginate beads, which will secrete growth factors capable of stimulating angiogenesis. (uea.ac.uk)

The cells terminally differentiate as they migrate to the surface (from the basal layer where the progenitor cells are located to the dead superficial surface). (wikipedia.org)
This process relies on the formation of a blastema, a pool of progenitor cells that is formed in response to amputation. (lu.se)
We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
The first part of the thesis (Paper I and II) explores reprogramming of adult human fibroblasts and human glial progenitor cells into GABAergic interneurons in 2D cultures. (lu.se)

In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin. (fsu.edu)
However, immunostaining of the decellularized cultures for cytoskeletal actin and b-tubulin showed that the decellularization protocol was unable to remove these cytoskeletal elements completely 15 . (researchsquare.com)
In detergent-extracted cytoskeletal preparations of cultured fibroblasts, p230 remained as an elaborate peripheral network that showed a distribution distinctly different from that of the major cytoskeletal structures, stress fibers, cortical myosin, vinculin, and intermediate filaments (IF). (rupress.org)

We also discuss several characterization methods that can be performed to validate the iTS cells derived using this approach. (edu.au)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Various decellularization methods have been described in literature, and they involve removal of cells and intracellular components using various chemical (such as acids, bases, and detergents), biological (such as enzymes, chelation agents, and toxins), or physical (such as freeze-thaw cycling) agents. (researchsquare.com)
In order to avoid protease-mediated degradation during processing, decellularization methods also need to include protease inhibitors to inactivate intracellular proteases released during the cell lysis 5,6 . (researchsquare.com)
Therefore, decellularization methods need to be tailored distinctly for different cell types. (researchsquare.com)
Methods of quantitation and characterisations of the cells. (pmf.hr)
Methods of cell growth synchronisation. (pmf.hr)
5. methods of cell quantitation: cell counting, determination of the cell viability by differential staining, flow cytometry. (pmf.hr)
Non-Cytopathic cells are identified by different methods like Immunofluroscence Haemagglutination, Haemadsorption, and adsorption methods. (biologyease.com)
The new technique is more than three times as effective as previous methods. (rdworldonline.com)
It grew new cells from 73 percent of the patients in a recent study, more than three times the success rate of previous methods and a first for early-stage cancers. (rdworldonline.com)
We also work on more sophisticated 3D culturing methods to model human brain development on an anatomical level with hESCs. (lu.se)

J2 cells were produced in F medium supplemented with 5 M Y and reverse-transfected with the siRNA library using Lipofectamine RNAiMax buy Dihydrotanshinone I reagent (Invitrogen) buy Dihydrotanshinone I at a final concentration of 20 nM. (cell-metabolism.com)

CCL2 has been shown to be increased and upregulated in patients with idiopathic pulmonary fibrosis, usual interstitial pneumonia, and bronchiolitis obliterans syndrome, and responsible for fibroblast production of TGF-beta and procollagen. (cdc.gov)
These results suggest that the epithelial-fibroblast co-culture system may be more reflective of the fibrotic disease state instead of monoculture systems, and may allow us to further elucidate the mechanism of MWCNT-induced fibrosis in vitro. (cdc.gov)
Thus, these results demonstrate a new pathogenic role of B cells and BAFF in fibrosis and systemic sclerosis. (ox.ac.uk)

By contrast, the described approach allows the generation of iTS cells from the adult cells of individuals with diverse genetic backgrounds, which are readily accessible to many laboratories around the world. (edu.au)
However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
The cells also hold valuable genetic information that could lead doctors to more informed treatment decisions and even tailor-made therapies for individual patients. (rdworldonline.com)
We generate iPS from human fibroblast with rare genetic metabolic disorders to explore our questions. (joyallab.com)

We hypothesize that gossypol, through its Bcl BclXL inhibitory exercise as a BH mimetic, synergistically interacts with ApoL TRAIL to mediate profound cytotoxicity and apoptosis of cultured thoracic cancer cells in vitro. (micrornaarray.com)
Cytotoxicity and Apoptosis Assays Cells had been seeded in properly microtiter plates at predetermined plating densities suitable for every cell line to accomplish confluence with the time cells have been to become handled with ApoL TRAIL. (micrornaarray.com)

While most of the decellularization protocols were originally developed for tissues, they have also more recently been adapted for CDMs from cultured cells. (researchsquare.com)
Cell and tissues cultures RI-excised from clone pHuIL-11/PMT supplied by Dr P (kindly. (mingsheng88.org)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)

To this aim, we use STED microscopy, phase holography microscopy, flow cytometry, immunocytochemistry and electron microscopy techniques to investigate the phenotype of a variety of cells cultured on nanowires and nanotubes. (lu.se)

CCL2 levels were significantly elevated in the basolateral chamber of MWCNT-exposed cells in co-culture, without similar changes in WI-38 monoculture or unexposed WI-38 grown in the co-culture system. (cdc.gov)

Amniotic epithelial cells transplantation in Parkinson model of rats was found to reverse the condition and prevent death in neurons (Kakishita et al. (scielo.org.ar)
2001) had found amniotic epithelial cells conditioned medium showed neurotrophic effect on rat embryonic day 18 (E18) cortical neurons. (scielo.org.ar)
Following the establishment of successful conversion protocols, we then introduce a 3D model for direct conversion that replicates the cell-cell interactions pivotal for physiological relevance, leading to an accelerated induction of functional neurons (Paper III). (lu.se)
We utilise monosynaptic tracing technology, using pseudotyped rabies vectors, in order to visualise the synaptic contacts formed between host neurons and graft human cells. (lu.se)
We have shown that human fibroblasts can be directly converted into functional and subtype-specific neurons using defined sets of transcription factors. (lu.se)
Current efforts focus on identifying the optimal starting cell population for neural conversion, identification of gene combinations that yield subtype specificity and analysis of function and integration of the reprogrammed neurons. (lu.se)

The capture and culture process starts with a microfluidic chip device that captures cancer cells as a blood sample is pumped across it. (rdworldonline.com)

Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
Co-culture of human being main epithelial cells with irradiated 3T3 fibroblast feeder cells (J2 cells) and the Rho kinase inhibitor Y-27632 (Y) allows for the unrestricted growth of cells of epithelial source by the process termed conditional reprogramming. (cell-metabolism.com)

22Rv1 cells were pre-treated with the pan-caspase inhibitor z-VAD(OMe)-fmk or vehicle (DMSO) for 1 h prior to treatment with the investigated drugs ( C , D ). PC-3, LNCaP, and MRC-9 cells were treated with the investigated drugs without pre-treatment with z-VAD(OMe)-fmk ( E ). Cells that appeared in the lower right and upper right quadrants were assumed to be apoptotic. (bioz.com)
Cultured gingival fibroblasts constitutively expressed BMP antagonist genes from the same subfamily, Grem2and and the Wnt inhibitor Grem2and and the Wnt inhibitor (Figure?4A). (bso14.org)
Open in a separate window Figure 4 A. Relative expression of BMP and BMP inhibitor genes by cultured gingival fibroblasts Expression normalized to averaged and expression. (bso14.org)

O. tsutsugamushi was isolated by using animal inoculation and L-929 mouse fibroblast cell culture techniques as described ( 9 ). (cdc.gov)
A widely used, alkaline detergent-based decellularization method has been developed for 3D NIH-3T3 mouse embryonic fibroblast cultures 10,11 . (researchsquare.com)
Qualified candidates will be trained with various laboratory techniques including retinal dissection, confocal microscopy imaging, laser-capture micro-dissection, real-time PCR, cell culture and mouse colony maintenance. (joyallab.com)
Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. (mdc-berlin.de)
The problem we have been concerned with is to find an in vitro correlate with tumorigenicity in a system of cell hybrids in the mouse. (biologists.com)
HFKs were co-cultured with irradiated (3,000 Rad) J2 cells (kindly provided by Dr. Richard Schlegel, Georgetown University or college) derived from mouse NIH 3T3 fibroblasts at a 1:4 HFK:J2 cell percentage, and sub-cultured when they reached 85% confluence. (cell-metabolism.com)

Our lab has developed advanced bioengineering techniques offering the generation of human organoids. (lu.se)

The applicant must have a good knowledge in cell biology approaches (cell culture, immunostaining) and also basic knowledge in microscopy techniques. (ibecbarcelona.eu)

The results suggest that p230 is part of a cell surface- and cytoskeleton-associated subplasmalemmal structure that may play an important role in cell surface-cytoskeleton interaction in various cells both in vitro and in vivo. (rupress.org)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
This includes long-term survival of transplanted cells in vivo to assess cell fate, maturation and integration, as well as functional behavioural assessment for graft function in vivo. (lu.se)
We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)

It involves incubation of the cultures in an alkaline detergent solution (20 mM NH 4 OH/0.5% Triton X-100, pH=8.0) to lyse cell membranes followed by digestion with DNase to remove DNA. (researchsquare.com)
Soon after an overnight incubation, cells had been handled with either gossypol or ApoL Avanafil TRAIL or hour pretreatment with gossypol followed by addition of ApoL TRAIL for a total of L per very well. (micrornaarray.com)

Cells from the supernatant of a h-LTBM C (cytospin preparations). (bremel.gr)

When keratinocytes Rabbit Polyclonal to CSTL1 are co-cultured with irradiated 3T3 fibroblast feeder cells (J2 cells) and growth factors [1, 4, 5], they undergo continuous replication over many decades resulting in their immortalization. (cell-metabolism.com)

The main cells of the lamina propria are the fibroblasts, which are responsible for the production of the extracellular matrix. (wikipedia.org)
Fibroblasts are the most common cells in extracellular matrix and are important for epithelial morphogenesis. (wikipedia.org)
These CDMs can be used to study cell interactions with the extracellular matrix (ECM) by reseeding cells in them. (researchsquare.com)

Transplantation of amniotic epithelial cells in various regions of central nervous system such as caudate nucleus (Bankiewicz et al. (scielo.org.ar)
The cell nucleus (blue) is indented by the nanowires. (lu.se)

We have also showed that this dual vector system can generate regulatable bulk cell lines (expressing high levels of TetR) that are able to modulate transgene expression either by varying doxycycline concentration and/or by varying the amount of CTetOE vector genomes per cell. (nih.gov)
Bulk doxycycline-responsive cell lines express high levels of the transgene with low amount of doxycycline and are phenotypically indistinct from its parental cells. (nih.gov)
These iTS cell lines can be established in 2-3 weeks and cultured long term over 50 passages. (edu.au)
This study aimed to evaluate the antitumor potential of naringin in silico and in vitro using two bladder cancer cell lines. (bvsalud.org)
Continuous cell lines. (biologyease.com)
We then put to use an antisense oligonucleotide technique, in which the energetic oligomers were picked from screening a panel of candidate molecules to down regulate the expression of bcl xL protein in the T and cell lines. (micrornaarray.com)

Anatomy20

Organisms8

Diseases5

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes28

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Health Care3

Endothelial8

Differentiation11

Embryonic cells1

Cancer-associated fibroblast2

Embryo Fibroblast Cells3

Isolation8

Primary18

Dermal fibroblasts8

Tissue22

Proliferation4

Esophageal1

Cytotoxic6

Lung4

Stimulates2

Collagen4

Stem23

Epithelium4

Vitro studies2

Growth factors secreted1

Cellular5

Morphology2

Somatic cell3

Murine1

Western blot1

Mammalian cell2

Proteins3

Adherent cells4

Epithelial cell1

Stromal1

Confluent2

Experiments3

Foreskin3

Transwell1

Monolayer10

Tumors1

Secrete2

Progenitor4

Cytoskeletal3

Methods12

Medium supplemented1

Fibrosis3

Genetic4

Apoptosis2

Tissues3

Electron1

CCL21

Neurons6

Microfluidic1

Reprogramming3

Inhibitor3

Mouse6

Organoids1

Microscopy Techniques1

Vivo4

Incubation2

Preparations1

Keratinocytes1

Extracellular matrix3

Nucleus2

Lines6