• Unlike conventionally applied faecal indicator bacteria (FIB), Bacteroides has very limited survival capabilities in the environment due to their anaerobic nature. (nwu.ac.za)
  • However, fecal indicator bacteria are not specific to human fecal contamination ( 6 ) and have a poor correlation with exposure risk to enteric viruses ( 4 , 7 - 9 ). (cdc.gov)
  • Various bacteria of the phylum Bacteroidetes have been proposed as the primary hosts of crAssphage, which was supported by recent findings that phage ΦCrAss001 from human feces could be isolated in Bacteroides ( 20 ). (cdc.gov)
  • Bacteroides bacteria were predominant in fecal samples. (nature.com)
  • Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. (wikipedia.org)
  • Studies indicate that long-term diet is strongly associated with the gut microbiome composition-those who eat plenty of protein and animal fats have predominantly Bacteroides bacteria, while for those who consume more carbohydrates the Prevotella species dominate. (wikipedia.org)
  • An alternative fecal indicator organism, Bacteroides, has been suggested because they make up a significant portion of the fecal bacterial population, have a high degree of host specificity that reflects differences in the digestive system of the host animal Over the past decade, real-time polymerase chain reaction (PCR) methods have been used to detect the presence of various microbial pathogens through the amplification of specific DNA sequences without culturing bacteria. (wikipedia.org)
  • This technique allows quantification of genetic markers that are specific to the host of the bacteria Bacteroides and allow detection of recent contamination. (wikipedia.org)
  • Do not use tools for digging the soil since they may have been previously contaminated with fecal bacteria unintentionally. (emsl.com)
  • Water samples are routinely collected by the New York State Department of Environmental Conservation in the bay and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. (usgs.gov)
  • Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. (usgs.gov)
  • We used traditional fecal indicator bacteria (FIB) and microbial source tracking (MST) methods to help identify source(s) of the FIB. (mdpi.com)
  • Human bacteroides, or cells of human specific fecal bacteria, were found in Tryon resident Eunice Whitmire's yard and crawl space, according to soil samples taken at the residence. (tryondailybulletin.com)
  • In layman's terms this can be described as cells of human specific fecal bacteria for a gram of the sample taken. (tryondailybulletin.com)
  • These mice showed a notable decrease in the abundance of Bacteroides vulgatus, beneficial gut bacteria that normally help block disease. (researchsquare.com)
  • Colonic inflammation was accompanied with a depletion of bacteria within the fecal compartment, a reduced surface area in which feces had direct contact with the colonic wall, increased thickness and spread of the mucus gap, and massive increases of bacterial concentrations in the crypt and interlaced compartments. (nih.gov)
  • Adhesive and infiltrative bacteria were observed in inflamed colon only, with dominant Bacteroides species. (nih.gov)
  • To test this hypothesis, the phylogenetic composition of faecal microbiomes was investigated by a 16S rRNA gene amplicon sequence-based approach for Bacteria and Archaea . (springer.com)
  • The phylogenetic composition of the Bacteria faecal microbiomes of baleen and toothed whales showed no significant differences at the phylum level. (springer.com)
  • The ingestion of prebiotics has repeatedly been shown to alter the composition of fecal bacteria. (preparedfoods.com)
  • It [also] suggests that gut bacteria might enhance currently used noninvasive fecal tests for the detection of colorectal polyps, and even that microbiome-modulating therapies might play a role in prevention of colorectal cancer ," said Gacesa, who won the award for best abstract in the meeting session. (medscape.com)
  • in particular, the bacteria Bacteroides fragilis and Alistipes finegoldii have been found to cause CRC in mouse models, explained Gacesa. (medscape.com)
  • To find out, researchers examined fecal samples from mice that died of V. vulnificus infection. (researchsquare.com)
  • Recent large-scale sewage contamination of the Vaal River in South Africa is an example of where microbial source tracking (MST) by means of Bacteroides host-associated assays could be applied advantageously in mitigation efforts. (nwu.ac.za)
  • To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. (cdc.gov)
  • Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands. (cdc.gov)
  • Thus, monitoring of human fecal contamination and identifying the source of contamination is an important approach to prevent transmission of gastroenteritis viruses for which humans are the only natural host (e.g., human norovirus) ( 1 ). (cdc.gov)
  • are widely used as indicators to assess the presence of human fecal contamination of environmental waters ( 2 - 5 ). (cdc.gov)
  • Culture methods are available to test for sewage contamination in buildings (M117 & M013) as well as new Bacteroides PCR based methods. (emsl.com)
  • Bacteroides is specific for fecal contamination from all sources-animals, birds, and human. (emsl.com)
  • Using Total Bacteroides (M095), it is now possible to determine the amount of fecal contamination from non-human and human sources. (emsl.com)
  • Using Human Bacteroides (M199) determines the amount of fecal contamination from human sources only. (emsl.com)
  • The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering South Oyster Bay, a shallow embayment on the southern shore of Long Island, New York. (usgs.gov)
  • Analysis revealed that the most substantial source of fecal contamination to South Oyster Bay was stormwater, particularly during the summer months. (usgs.gov)
  • Canine contamination was prolific at source sites but was associated with low fecal coliform concentrations in the winter months. (usgs.gov)
  • Based a classification scheme developed to convey the degree of fecal contamination to stakeholders and resource managers, the Cedar Creek near Bay Place and Unqua Lake Culvert sites were identified as locations that contribute substantial fecal contamination to South Oyster Bay. (usgs.gov)
  • For almost 40 years, Bonde's (1966) attributes of an ideal indicator have served as an effective model of how a fecal contamination index for public health risk and treatment efficiency should function ( Box 4-1 ). (nationalacademies.org)
  • Bacteroides species play multiple roles within the human gut microbiome. (wikipedia.org)
  • Gas chromatography/mass spectrometry, liquid chromatography/tandem mass spectrometry, clinical chemistry analysis, dual-energy X-ray absorptiometry, and 16S pyrosequencing were used to characterize blood metabolic, clinical, and fecal microbiome systems, respectively. (hindawi.com)
  • Fecal microbiome studies conducted after the first four weeks revealed few differences in the gut microbiomes of the dogs. (phys.org)
  • These were then linked with Dutch microbiome project data sourced from fecal samples of 8208 individuals taken between 2012 and 2015. (medscape.com)
  • This allowed us to associate gut microbiome compositions and functions to detailed histological information about precancerous lesions and CRC, including when lesions were detected relative to fecal sampling [and a reading of the gut microbiome]," Gacesa explained. (medscape.com)
  • When the researchers looked at microbiome diversity in people who had experienced precancerous colonic lesions 1 to 5 years before fecal sampling, they found that diversity was lower compared to controls. (medscape.com)
  • Hygienic practices, including disinfection of environmental surfaces, are important to reduce exposure to pathogens that spread through fecal-oral transmission. (cdc.gov)
  • Bacteroides species also benefit their host by excluding potential pathogens from colonizing the gut. (wikipedia.org)
  • Most respiratory infections die out of the environment fairly quickly but faecal pathogens tend to be more persistent. (thepigsite.com)
  • The strongest correlation between FIO density in sand and water was fecal Bacteroides CCE, followed by enterococci CFU, Clostridium spp. (cdc.gov)
  • Dogs that ate a low-protein, high-carbohydrate diet had higher abundances of Bacteroides uniformis and Clostridium butyricum. (phys.org)
  • Genetic analysis revealed that Lachnospiraceae and genus Bacteroides gut microbial groups were most affected by diet, with the lowest relative abundance following consumption of the rye diet. (petfoodindustry.com)
  • Bifidobacteria are not alone in their ability to consume HMOs, as members of the genus Bacteroides are known to consume some types of HMOs [ 18 ]. (biomedcentral.com)
  • In particular, diminished fecal abundance of Faecalibacterium prausnitzii and abnormalities in both directions in the abundance of the Bacteroides genus have been identified. (medscape.com)
  • To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. (wjgnet.com)
  • Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bifidobacteria using real-time polymerase chain reaction. (wjgnet.com)
  • Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology. (wjgnet.com)
  • By analyzing patients' fecal samples, the team found the resistant starch group had a different microbiota composition and functionality compared with the control. (eurekalert.org)
  • Fecal samples were obtained from patients with Crohn's disease in a pilot diet crossover trial comparing the effects of a specific carbohydrate diet (SCD) versus a low residue diet (LRD) on the composition and complexity of the gut microbiota and resolution of IBD symptoms. (scdrecipe.com)
  • Scientists examined how whole grain rye, wheat and oats influenced fecal microbiota composition, short-chain fatty acids production and diet digestibility. (petfoodindustry.com)
  • We hypothesized that oral doxycycline would alter the composition of the GM (as evidenced by changes in the fecal microbiota [FM]) in female C57BL/6NCrl mice, and that the GM would return to baseline when the drug was temporarily discontinued. (biomedcentral.com)
  • The analysis determined the composition, function, and genomic profiles of gut microbiota in participants who developed precancerous colorectal lesions before fecal sampling from 2000 to 2015, and in those participants who developed lesions after fecal sampling, between 2015 and 2022. (medscape.com)
  • We assessed alterations in gut microbiota composition, fecal and blood inflammatory makers, and gut-related symptoms before, during and after the GMP feeding period. (bvsalud.org)
  • When the team transplanted fecal microbiota from resistant starch-treatment patients to mice fed with a high-fat high-cholesterol diet, the mice saw a significant reduction in liver weight and liver triglyceride levels and improved liver tissue grading compared with mice that received microbiota from the control group. (eurekalert.org)
  • Although Bacteroides species are anaerobic, they are transiently aerotolerant and thus can survive in the abdominal cavity. (wikipedia.org)
  • Because they are anaerobic, Bacteroides will not multiply in the environment. (emsl.com)
  • Bacteroides species make up the majority of mammalian intestinal bacterial communities. (nwu.ac.za)
  • Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. (wikipedia.org)
  • Bacteroides species are normally mutualistic, making up the most substantial portion of the mammalian gastrointestinal microbiota, where they play a fundamental role in processing of complex molecules to simpler ones in the host intestine. (wikipedia.org)
  • however, the main sources of energy for Bacteroides species in the gut are complex host-derived and plant glycans. (wikipedia.org)
  • In general, Bacteroides are resistant to a wide variety of antibiotics-β-lactams, aminoglycosides, and recently many species have acquired resistance to erythromycin and tetracycline. (wikipedia.org)
  • This high level of antibiotic resistance has prompted concerns that Bacteroides species may become a reservoir for resistance in other, more highly pathogenic bacterial strains. (wikipedia.org)
  • Some Bacteroides species are associated with Crohn's disease, appendicitis and inflammatory bowel disease. (wikipedia.org)
  • Bacteroides species' main source of energy is fermentation of a wide range of sugar derivatives from plant material. (wikipedia.org)
  • In particular, the treatment-group patients had a lower level of Bacteroides stercoris, a key bacterial species that can affect fat metabolism in the liver through its metabolites. (eurekalert.org)
  • Recent studies showing an association between fecal indicator organisms (FIOs) in sand and gastrointestinal (GI) illness among beachgoers with sand contact have important public health implications because of the large numbers of people who recreate at beaches and engage in sand contact activities. (cdc.gov)
  • Our results show the association between fecal ETBF and CRC, and we suggest that detection of ETBF may be a potential marker for colorectal cancer diagnosis. (biomedcentral.com)
  • image: The presence of Bacteroides vulgatus led to colitis in the mouse colon (left). (eurekalert.org)
  • The team found that roughly 40 percent of ulcerative colitis patients show an overabundance of proteases - enzymes that break down other proteins - originating from the gut resident Bacteroides vulgatus. (eurekalert.org)
  • Bacteroides such as Bacteroides thetaiotaomicron converts these sugars to fermentation products which are beneficial to humans. (wikipedia.org)
  • We colonized germ-free mice with Bacteroides thetaiotaomicron . (cdc.gov)
  • Water quality, weather and environmental factors associated with fecal indicator organism density in beach sand at two recreational marine beaches. (cdc.gov)
  • Unusual in bacterial organisms, Bacteroides membranes contain sphingolipids. (wikipedia.org)
  • 4.3.2 Problems Associated with Using Fecal Coliforms as Indicators of Pathogenic Organisms. (taylorfrancis.com)
  • Generally, a positive effect would be to see increases in the populations of lactobacilli and bifidobacteria and decreases in less desirable organisms such as clostridia and protein-degrading bacteroides. (preparedfoods.com)
  • The recent success gut microbial genes, obtained after has fantastic potential, it has only of faecal microbiota transplantation, sequencing whole faecal microbiota been about 10 years since the sci- especially in the context of Clostridi- metagenomes from 124 European indi- entific community first realized its im- um difficile infection, argues for such viduals [4]. (who.int)
  • Coliforms, E.coli , fecal streptococci and enterococci can grow in water, soil, sediments and on vegetation in uncontaminated environments. (emsl.com)
  • 121 4.8.3 Enumeration of Fecal Streptococci/Enterococci. (taylorfrancis.com)
  • Shotgun metagenomics sequencing of the fecal DNA in the pediatric subjects revealed diminished coverage of the butanoate pathway (abundance normalized to controls of 1 ± 0.48 versus 0.72 ± 0.33 in ERA, p = 0.037). (medscape.com)
  • The human-associated Bacteroides marker was the most frequently detected microbial source tracking marker in South Oyster Bay (50 percent positive detections). (usgs.gov)
  • The aim of this study was to investigate the frequency of ETBF in stool samples of CRC patients and healthy volunteers to find the possible correlation between fecal ETBF with CRC. (biomedcentral.com)
  • We evaluated the effect of doxycycline treatment by sequencing the V4 hypervariable region of the 16S rRNA gene from fecal samples collected during a 4 week course of treatment at a dose of 2 mg/ml in the drinking water. (biomedcentral.com)
  • Some individuals harbour Bacteroides intestinalis that is incapable of consuming polymeric xanthan gum but grows on oligosaccharide products generated by the Ruminococcaceae. (nature.com)
  • Over the past few decades, several viruses (e.g., human polyomavirus, Aichi virus, norovirus, and human adenovirus) have been studied as human fecal indicators for the detection of sewage-contaminated source and drinking water ( 10 - 13 ). (cdc.gov)
  • In addition, we tested stool samples from healthy persons (n = 173), respiratory samples (n = 113), and animal fecal specimens (n = 68) and further sequenced positive samples. (cdc.gov)
  • Methods We obtained fecal specimens from 30 children with treatment-naïve enthesitis-related arthritis (ERA) and 19 healthy controls, as well as specimens from 11 adult patients with longstanding SpA and 10 adult healthy controls. (medscape.com)
  • One study has measured the amount of Bacteroides by using qPCR to quantify the host-specific 16S rRNA genetic marker. (wikipedia.org)
  • Recently, a new DNA bacteriophage was discovered by computational analysis of publicly accessible human fecal metagenomics data and was named crAssphage, referring to the Cross-Assembly software that was used for its discovery ( 18 ). (cdc.gov)
  • A subset of the pediatric fecal samples was subjected to shotgun metagenomics sequencing. (medscape.com)
  • However, current methods of faecal sampling in a rural setting for diagnosis are not consistently sufficient for the preservation of Salmonella spp. (imperial.ac.uk)
  • Davis said the town does understand that some of the soil samples came back positive for human bacteroides, a sign of human feces, which was expected and validates what the town knows, that obviously, there has been spillage at Whitmire's property. (tryondailybulletin.com)
  • In cases where Bacteroides can move outside the gut due to gastrointestinal tract rupture or intestinal surgery, Bacteroides can infect several parts of the human body. (wikipedia.org)
  • Higher levels of beta-glucuronidase may be associated with an imbalanced intestinal microbiota profile, as well as higher circulating estrogens and lower fecal excretion of estrogens in premenopausal women. (doctorsdata.com)
  • The results revealed no changes in fecal microbiota, subtle effects on systemic and intestinal immune makers, and no changes in gut-related symptoms during and after the GMP supplementation. (bvsalud.org)
  • Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. (wjgnet.com)
  • A systematic literature review was performed identifying recurring themes with regard to challenges and limitations faced by researchers applying Bacteroides related assays to environmental water samples. (nwu.ac.za)
  • The researchers collected fecal samples at the end of each diet period. (petfoodindustry.com)
  • This study aims to demonstrate the effect of oral doxycycline on fecal microbiota of mice. (biomedcentral.com)
  • The goal of this study was to develop an optimized methodology for fecal sample processing and to detect changes in the gut microbiota of patients with Crohn's disease receiving specialized diets. (scdrecipe.com)
  • Cluster 1 had a urine cluster pattern that was distinct from fecal, whereas cluster 2 was similar to fecal. (nature.com)
  • The normal colonic flora was organized in three distinct compartments (crypt, interlaced, and fecal), each with different bacterial compositions. (nih.gov)
  • Along with measurement of SARS-CoV-2 RNA in this step, several laboratory controls should also be measured, including matrix recovery controls, human fecal normalization, quantitative measurement controls, and controls to assess molecular method inhibition. (cdc.gov)
  • upon opening the abdomen, fecal-contents are observed in abdominal cavity and the area is copiously irrigated. (cdc.gov)
  • Faecal pollution of water has long been an issue of great concern due to the potential health risks associated with faeces. (nwu.ac.za)
  • Yet, factors that influence fecal pollution in beach sand remain unclear. (cdc.gov)
  • All detections of the canine-associated Bacteroides marker were in samples collected during the winter season and were associated with fecal coliform concentrations below the reporting limit, indicating that birds are not a persistent source of fecal coliform to South Oyster Bay. (usgs.gov)
  • Overall, we detected crAssphage in 71.4% of outbreak stool samples, 48%-68.5% of stool samples from healthy persons, 56.2% of environmental swabs, and 60% of hand rinse samples, but not in human respiratory samples or animal fecal samples. (cdc.gov)
  • The absence of fecal coliform and human markers in groundwater samples collected throughout the larger study area indicates that water from cesspools or septic tanks do not contribute fecal coliform to the bay. (usgs.gov)
  • The soil samples on the left side yard were negative for human bacteroides and one of the two samples on the right side of the yard was positive at 1,774 cell equivalents/gram of soil," said Salmon's analysis of the report. (tryondailybulletin.com)
  • The crawl space sample was positive for human bacteroides with a cell equivalent count/gram of soil at 628. (tryondailybulletin.com)
  • In laboratory studies using fecal culture, they consistently display prebiotic effects, but these have not always been reproduced in human volunteer trials. (preparedfoods.com)
  • High-end consumption of soluble fiber (e.g. inulin) and supplementation with Lactobacillus acidophilus may be inconsequentially associated with lower fecal β-G. (doctorsdata.com)
  • We conclude that supplemental complex carbohydrates that increase Bifidobacteria and Lactobacilli, without increasing the deleterious Bacteroides , are most likely promoting cardiometabolic and immunological health in obese subjects. (frontiersin.org)
  • DNA extraction from fecal samples using a column based method provided consistent results. (scdrecipe.com)
  • The noninvasive fecal immunochemical test (FIT), recently shown to be a preference among patients, produces a high number of false-positive results, leading to many unnecessary colonoscopies. (medscape.com)
  • Another study showed a 5.6-times higher risk of osteoporosis fractures in the low Bacteroides group of Japanese postmenopausal women. (wikipedia.org)
  • This treatment resulted in a significant improvement in HOMA as a measure of insulin resistance in the Fecal-Microbial-Transplant-Low-Fermentable fibre group (FMT-LF), with no changes in any of the other groups. (drsharma.ca)
  • The Bacteroides group showed greater thickness of the gray matter in the frontal cortex and insula, brain regions involved with complex processing of information. (medicalxpress.com)
  • They also rated higher levels of negative feelings such as anxiety, distress and irritability after looking at photos with negative images than did the Bacteroides group. (medicalxpress.com)