• 2003). Selective Cellular uptake of fatty acids and following storage in the form of disruption of Pparγ2 or adipocyte-speci﫿c Pparγ knockout leads TGs in adipocytes are key steps in lipid storage. (deepdyve.com)
  • High blood concentrations of nonesterified fatty acids (NEFAs) provoke various metabolic disorders and are associated with mammary tissue injury and decreased milk production in dairy cows. (bvsalud.org)
  • In hepatoma cell lines, fatty acids increase Hilpda expression and protein levels. (wur.nl)
  • RESEARCH DESIGN AND METHODS- We used CL-316,243, a β3 adrenergic receptor agonist, to acutely elevate blood free fatty acids and to study its effect on insulin secretion in vivo. (diabetesjournals.org)
  • Fatty acids are involved in a diverse array of physiological functions in a variety of tissues. (diabetesjournals.org)
  • FABPs are a class of cytoplasmic proteins that bind long chain fatty acids. (thermofisher.com)
  • They are abundantly present in various cell types and seem to play an important role in the intracellular utilization of fatty acids. (thermofisher.com)
  • Lipogenesis is a process involved in lipid storage, the rate limiting step of which is fatty acids desaturation catalyzed by stearoyl-CoA desaturase (SCD). (edu.pl)
  • Emerging evidence has demonstrated that saturated fatty acids prime pro-IL-1β production and inflammasome-mediated IL-1β activation is critical in obesity-associated insulin resistance (IR). (elsevierpure.com)
  • Two decades later, the prostaglandins were deduced to be a family of related compounds that contain 20-carbon polyunsaturated fatty acids with a cyclopentane ring, as depicted below. (medscape.com)
  • Among the peripheral signals that are generated to regulate the uptake of food, signals from adipose tissue are of major relevance and involve the maintenance of energy homeostasis through processes such as lipogenesis, lipolysis, and oxidation of fatty acids. (wjgnet.com)
  • A fat tolerance test evidenced delayed plasma triglyceride clearance and greater transient availability of non-esterified fatty acids (NEFA) during the post-prandial state in the apoCIII mice plasma. (biomedcentral.com)
  • In lipodystrophy there is insufficient adipose tissue to absorb the postprandial influx of fatty acids, so these fatty acids will again be directed to other tissues. (cambridge.org)
  • Björntorp , P ( 1994 ) Fatty acids, hyperinsulinemia, and insulin resistance: which comes first? (cambridge.org)
  • Dietary fatty acids (FA) are components of the lipids, which contribute to membrane structure, energy input, and biological functions related to cellular signaling and transcriptome regulation. (nature.com)
  • Previous studies already demonstrated that some fatty acids are linked with inflammatory response, preventing metabolic diseases. (nature.com)
  • Fatty acids are the main compound of lipids, which are a class of molecules present in animals and vegetal cell types. (nature.com)
  • Its oils are rich sources of unsaturated fatty acids, such as monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids, which are previously associated with the prevention of health disorders because of their anti-inflammatory effects and cell membrane properties and structure 2 . (nature.com)
  • Essential FA, mainly polyunsaturated fatty acids (PUFA), may modulate gene expression in diverse biological processes thought regulating transcription factors (TF), including peroxisome proliferator receptors (PPAR) , liver X receptors (LXR) , and sterol regulatory element-binding proteins ( SREBP ) 3 . (nature.com)
  • Fatty acids and glucose serve as substrates for energy production. (medscape.com)
  • Low plasma levels of the anti-inflammatory factor adiponectin characterize obesity and insulin resistance. (diabetesjournals.org)
  • Scientists from the University of North Carolina School of Medicine discovered that the anti-inflammatory protein NLRP12 normally helps protect mice against obesity and insulin resistance when they are fed a high-fat diet. (integrativepractitioner.com)
  • The study , published in Cell Host & Microbe , showed that NLRP12's anti-inflammatory effect promotes the growth of a "good" family of gut-dwelling bacteria, called Lachnospiraceae , that produce small molecules butyrate and propionate, which in turn promote gut health and protect mice against obesity and insulin resistance. (integrativepractitioner.com)
  • Despite the wealth of data concerning adipose tissue metabolic alterations in conditions of obesity and insulin resistance, the involvement of RNA splicing and other post-transcriptional RNA processing events in these processes remains poorly studied. (unil.ch)
  • We particularly focus on how these proteins participate in the onset and development of diet-induced obesity and insulin resistance. (unil.ch)
  • Evidence suggests that obesity and insulin resistance are the major factors that contribute to the development of NAFLD. (wjgnet.com)
  • To this end we used a HILPDA tissue-specific knockout mice model in macrophages and hepatocytes generated by using LysM-Cre and Alb-Cre transgenic mice, respectively. (wur.nl)
  • To develop our research, we use tissue-specific knockout mice, as well as cellular models of adipocytes. (unil.ch)
  • Because the AIF knockout shifted the metabolite balance--decreased ATP, decreased NAD, and increased AMP--activation of AMPKinase might have stimulated catabolic processes including glucose uptake and fatty acid oxidation. (bruker.com)
  • My research is primarily focused on understanding the regulation of mitochondrial bioenergetics, with a particular interest in studying fatty acid oxidation (breakdown of fat yielding energy) in skeletal and cardiac muscle. (uoguelph.ca)
  • CDK4 Phosphorylates AMPKalpha2 to Inhibit Its Activity and Repress Fatty Acid Oxidation. (unil.ch)
  • Leptin and adiponectin can augment the oxidation of fatty acid in liver by activating the nuclear receptor super-family of transcription factors, namely peroxisome proliferator-activated receptor (PPAR)-α. (wjgnet.com)
  • To study the role of fat metabolism in GSD type V, Andersen et al manipulated the availability of free fatty acid for oxidation during exercise in 10 patients with the disease. (medscape.com)
  • These mice were also resistant to diet-induced obesity and diabetes when fed a high fat diet. (bruker.com)
  • A multisystem AIF knockout mouse, the Harlequin mouse, was also found to have the same qualities of improved insulin sensitivity, glucose tolerance, and resistance to diet-induced obesity and diabetes. (bruker.com)
  • Ffar1 +/+ (wild-type) and Ffar1 −/− (knockout) mice were placed on two distinct high-fat diets to study their response to diet-induced obesity. (diabetesjournals.org)
  • Decreased insulinaemia due to lowered GSIS protects B-Atgl-KO mice from diet-induced obesity, improves insulin sensitivity, increases lipid mobilisation from WAT and causes BAT activation. (omicsdi.org)
  • We previously hypothesized that apoCIII overexpression would compromise fatty acid delivery to adipose tissues and would thus contribute to resistance to diet-induced obesity, similarly to what was described for overexpression of apoCI [ 15 ]. (biomedcentral.com)
  • Therefore, the role of apoCIII on adiposity seems to be quite complex because both apoCIII overexpression or disruption results in more severe diet-induced obesity than wild type mice [ 14 ]. (biomedcentral.com)
  • Non-alcoholic fatty liver disease (NAFLD) is a silent pandemic associated with obesity and the metabolic syndrome, and also increases cardiovascular- and cirrhosis-related morbidity and mortality. (nih.gov)
  • However, knocking down the elevated ChREBP expression in liver of obese mice improves insulin sensitivity and metabolic syndrome. (grantome.com)
  • Parental metabolic syndrome epigenetically reprograms offspring hepatic lipid metabolism in mice. (theopenscholar.com)
  • One of our groundbreaking discoveries was the identification of founder mutations in the DYRK1B gene, underlying atherosclerosis, metabolic syndrome, and fatty liver disease. (yale.edu)
  • Fatty acid synthesis (de novo lipogenesis, DNL) is elevated in liver in obesity and type 2 diabetes and is usually associated with insulin resistance. (grantome.com)
  • Metabolomics analysis suggested that GH influences the serum and organ FFA flux through various aspects of lipid metabolism (e.g., lipogenesis, lipolysis and free fatty acid (FFA) uptake), especially the desaturase metabolic processes. (tus.ac.jp)
  • We measured body energy balance, tissue capacity to store exogenous lipids, lipogenesis and lipolysis rates in non-transgenic and apoCIII overexpressing mice fed a HFD during two months. (biomedcentral.com)
  • Lipogenesis rates were similar, while exogenous lipid retention was increased in perigonadal (2-fold) and brown adipose tissues (40 %) of apoCIII mice. (biomedcentral.com)
  • Our studies in mice have shown that this upregulation results in mTOR activation, lipogenesis and development of NASH and dyslipidemia. (yale.edu)
  • In adipocyte Senp2-de﫿ciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)
  • 2010). Senp2 also regulates fatty acid metabolism in skeletal Downloaded from https://academic.oup.com/jmcb/article-abstract/10/3/258/4763638 by Ed 'DeepDyve' Gillespie user on 26 June 2018 Senp2 regulates adipose lipid storage by de-SUMOylation of Setdb1 j 259 muscle (Koo et al. (deepdyve.com)
  • Despite enhanced lipid and cholesterol biosynthesis, Insig1 KO mice had similar systemic metabolism and insulin sensitivity to Het/WT littermates. (nih.gov)
  • Abnormal lipid metabolism has been associated with a wide range of chronic and infectious diseases including non-alcoholic fatty liver disease, viral hepatitis C infection, atherosclerosis, diabetes, and cancer. (wur.nl)
  • Our results support the major role of PPARα in regulating hepatic lipid metabolism, highlight the more modest effect of PPARα activation on gene regulation in human liver compared to mouse liver, and indicate that PPARα may have a suppressive effect on DNA synthesis in human liver. (wur.nl)
  • Type 2 diabetes mellitus (T2DM) is a progressive metabolic disease characterized by pancreatic β-cell dysfunction and peripheral insulin resistance, leading to defects in glucose metabolism and chronic low-grade inflammation. (frontiersin.org)
  • Moreover, decreased insulinaemia in B-Atgl-KO mice was associated with increased energy expenditure, and lipid metabolism in brown (BAT) and white (WAT) adipose tissues, leading to reduced fat mass and body weight. (omicsdi.org)
  • Importantly, the alternative splicing of the LMNA gene was highly sensitive to the levels of specific Serine-Arginine rich (SR) proteins (5) , and produced several splicing isoforms that affect adipose tissue metabolism and aging in opposite manners, with Lamin A and Progerin promoting energy expenditure and aging, while Lamin C favors slower metabolism and moderately slows the aging process in mice (4) . (unil.ch)
  • Conclusion: Ethanol-fed SOD -/- mice exhibited lower alcohol dehydrogenase activity and lack of CYP2E1 inducibility, thereby causing decreased ethanol metabolism compared with wild-type mice. (elsevierpure.com)
  • We have developed expertise in in vivo investigation of lipid and glucose metabolism, insulin secretion and sensitivity, and vascular biology and in human physiological studies, leading to discovery of attractive drug targets that have been either patented or being investigated for their utility in treatment of fatty liver disease and diabetes in 2 clinical trials in the outlier populations of Fars/Iran. (yale.edu)
  • Although less fat stor- adqcKO pose lipid storage in adipocyte-speci﫿c Senp2 knockout mice fed age was shown in Senp2 adipose tissues, the increased with high-fat diets (HFD). (deepdyve.com)
  • Similarly, in diet-induced NASH mice, HILPDA hepatocyte deficiency modestly yet significantly reduced liver triglyceride accumulation and plasma ALT levels. (wur.nl)
  • Insulin resistance in older PTG heterozygous mice correlates with a significant increase in muscle triglyceride content, with a corresponding attenuation of insulin receptor signaling. (jci.org)
  • To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
  • While wild-type mice exhibited fatty liver after ethanol administration, SOD -/- mice showed no evidence of ethanol-induced steatosis, although triglyceride levels were elevated in both groups of knockout mice. (elsevierpure.com)
  • These mice are generated by transplanting human hepatocytes into albumin enhancer-driven urokinase-type plasminogen activator transgenic/severe combined immunodeficiency (uPA/SCID) mice, leading to replacement of the host hepatocytes. (wur.nl)
  • Transgenic mice overexpressing human apoCIII have marked elevated TG and non-esterified fatty acid (NEFA) levels [ 21 ]. (biomedcentral.com)
  • Here, we explored the role of NPC1L1 in colorectal tumorigenesis in vivo by using transgenic mice. (biomedcentral.com)
  • B-Atgl-KO male mice fed an HFD showed reduced insulinaemia, glycaemia in the fasted and fed states and after glucose challenge, as well as enhanced insulin sensitivity. (omicsdi.org)
  • In various mouse and rat models of obesity and type 1 and 2 diabetes mellitus, eCBs generated in various renal cells activate CB 1 receptors and contribute to the development of oxidative stress, inflammation, and renal fibrosis. (degruyter.com)
  • Exceeding the capacity to store TG in adipocytes occurs in obesity and is often accompanied by deposition of TG in other tissues and metabolic diseases, such as diabetes mellitus or non-alcoholic fatty liver disease. (elifesciences.org)
  • In mice, we showed that NLRP12 reduces inflammation in the gut and in adipose fat tissues. (integrativepractitioner.com)
  • Moreover, we hypothesized that these mice would have accumulation of TGs in other tissues, such as the liver or skeletal muscle, resulting in lipotoxicity and metabolic derangements, such as insulin resistance or fatty liver disease. (elifesciences.org)
  • This view of the link between adipose tissue and insulin resistance emphasises the important role of adipose tissue in 'buffering' the daily influx of dietary fat entering the circulation and preventing excessive exposure of other tissues to this influx. (cambridge.org)
  • ChREBP has been studied mainly in liver and pancreatic cells where it regulates fatty acid synthesis and glycolysis. (grantome.com)
  • Our data indicate that GHR regulates the crosstalk between adipose tissue and liver through fatty acid desaturation. (tus.ac.jp)
  • Ting and colleagues in recent years have published studies showing that mice lacking the NLRP12 gene are highly susceptible to excessive inflammation, including experimental colon inflammation and associated colon cancer. (integrativepractitioner.com)
  • The absence of NLRP12 in these mice led to increased signs of inflammation in the gut and in fat deposits, but it wasn't clear how this led to extra weight gain until the researchers moved the animals from one facility to another. (integrativepractitioner.com)
  • While mature-onset obesity is evident in LFD-IL-1RI(-/-) mice, the additional metabolic insult of HFD was required to drive adipose inflammation and systemic IR. (elsevierpure.com)
  • Therefore, COX - also known as prostaglandin-endoperoxide synthase (PTGS), fatty acid COX, prostaglandin H (PGH) synthase, and EC 1.14.99.1 - is implicated in the production of fever, inflammation, and pain. (medscape.com)
  • However, when obesity and inflammation are sustained, these adaptive homeostatic mechanisms fail, leading to WAT dysfunction characterised by impaired secretion of adipokines, abnormal lipid storage and adipogenesis, exacerbated fibrosis deposition and insulin resistance. (springer.com)
  • NPC1L1 −/− mice had significant lower intestinal inflammation scores and expressed inflammatory markers p-c-Jun, p-ERK and Caspase-1 p20 lower than wild-type. (biomedcentral.com)
  • NPC1L1 knockout also reduced lymphadenectasis what may be caused by inflammation. (biomedcentral.com)
  • NPC1L1 knockout decreasing plasma lipid, especially cholesterol, to reduce inflammation and decreasing β-catenin, p-c-Jun and p-ERK may be involved in the mechanism. (biomedcentral.com)
  • Senp2 knockout mice exhibited a lipodystrophy-like phenotype. (deepdyve.com)
  • In the present study, we characterized the phenotype of HSD17B13-knockout (HSD17B13KO) mice deficient in Hsd17b13. (richardvigilantebooks.com)
  • Project was supported by the " Role of synaptic plasticity impairments in the development of asocial phenotype in mouse models of autism (NCN 2015/18/E/NZ4/00600)" grant. (edu.pl)
  • Studies have shown that polyunsaturated fatty acid arachidonic acid (AA) promotes prostate cancer progression. (bioxorio.com)
  • Their distinct biosynthetic activity includes an endoperoxidase synthase reaction that oxygenates and cyclizes polyunsaturated fatty acid precursors (eg, arachidonic acid) to form prostaglandin G 2 (PGG2), and a peroxidase reaction that converts PGG2 to prostaglandin H 2 (PGH2), as shown below. (medscape.com)
  • It was previously reported that FFAR1 knockout ( Ffar1 −/− ) mice were resistant to high-fat diet-induced hyperinuslinemia, hyperglycemia, hypertriglyceridemia, and hepatic steatosis. (diabetesjournals.org)
  • however, Ffar1 −/− mice are not protected from high-fat diet-induced insulin resistance or hepatic steatosis. (diabetesjournals.org)
  • We found that the lipid accumulation in the adipose tissue of DKO was further aggravated compared to AKO, while the insulin resistance and hepatic steatosis were abolished in DKO, compared to LKO. (tus.ac.jp)
  • The experiment returned the AIF knockout mice to normal glucose tolerance and plasma peak insulin levels. (bruker.com)
  • Although young PTG heterozygous mice initially demonstrate normal glucose tolerance, progressive glucose intolerance, hyperinsulinemia, and insulin resistance develop with aging. (jci.org)
  • Experiments repeated with liver-specific AIF knockout mice showed the same result--increased glucose tolerance and insulin sensitivity. (bruker.com)
  • They engineered AIF knockout mice with muscle- or liver-specific OxPhos deficiency and confirmed that the animals were a valid model for OxPhos dependency of insulin resistance. (bruker.com)
  • We generated an inducible liver-specific Reptin knockout ( Repin LKO ) mouse model. (nih.gov)
  • To this end, we used the liver-specific insulin receptor knockout (LIRKO) mouse, a unique nondietary model manifesting 3 hallmarks that confer high risk for the development of NAFLD: hyperglycemia, insulin resistance, and dyslipidemia. (theopenscholar.com)
  • Adipocyte Senp2 de﫿ciency resulted in less adipose lipid storage accompanied by an ectopic fat accumulation and insulin resistance under high-fat diet feed- ing. (deepdyve.com)
  • adqcKO Mechanistically, adipocyte Senp2 de﫿ciency caused the downregula- Senp2 mice exhibit an ectopic lipid accumulation and tion of Pparg and Cebpa as well as their downstream target genes insulin resistance related to lipid storage. (deepdyve.com)
  • In diet-induced obese mice, HILPDA deficiency in macrophages markedly reduced lipid accumulation in macrophages yet it did not alter any measured inflammatory or metabolic parameters. (wur.nl)
  • In addition, high-fat diet induced comparable levels of lipid accumulation in livers of Ffar1 +/+ and Ffar1 −/− mice. (diabetesjournals.org)
  • Moreover, in the heart of SCD4-/- mice fed HFD, decreased lipid accumulation was accompanied by higher rate of lipolysis, increased mitochondria quality control, lower activity of NADH dehydrogenase and level of reactive oxygen species. (edu.pl)
  • Accumulation of triacylglycerol in skeletal muscles and in liver is associated with insulin resistance. (cambridge.org)
  • We will create mice that overexpress or lack ChREBP selectively in adipocytes. (grantome.com)
  • Aim 2 is to determine whether absence of ChREBP selectively in adipocytes causes systemic insulin resistance. (grantome.com)
  • Because reduced ChREBP expression in adipose tissue of obese humans correlates highly with insulin resistance, understanding the mechanisms that regulate ChREBP in adipocytes could lead to novel therapeutic approaches to prevent and treat type 2 diabetes. (grantome.com)
  • We hypothesized that mice lacking TG storage in adipocytes would result in excess TG storage in cell types other than adipocytes and severe lipotoxicity accompanied by metabolic disease. (elifesciences.org)
  • To test this hypothesis, we selectively deleted both TG-synthesis enzymes, DGAT1 and DGAT2, in adipocytes (ADGAT DKO mice). (elifesciences.org)
  • We generated mice lacking both known TG synthesis enzymes, DGAT1 and DGAT2 1 , 2 , in adipocytes. (elifesciences.org)
  • Model systems to study PPARα in human liver vary from hepatoma cell lines, human primary hepatocytes, human precision cut liver slices, and mice expressing human PPARα. (wur.nl)
  • Fenofibrate increased the size of the mouse but not human hepatocytes and tended to reduce steatosis in the human hepatocytes. (wur.nl)
  • Quantitative PCR indicated that induction of PPARα targets by fenofibrate was less pronounced in the human hepatocytes than in the residual mouse hepatocytes. (wur.nl)
  • We worked on a mouse model that overexpresses the well-known oncogene RAS in hepatocytes. (genengnews.com)
  • However, Ffar1 +/+ and Ffar1 −/− mice had similar weight, adiposity, and hyperinsulinemia on high-fat diets, and Ffar1 −/− mice showed no improvement in glucose or insulin tolerance tests. (diabetesjournals.org)
  • IL-1RI(-/-) mice developed glucose intolerance and IR after 6 mo HFD compared with 3 mo HFD, coincident with enhanced weight gain, hyperinsulinemia, and hyperleptinemia. (elsevierpure.com)
  • Methods: We generated muscle-specific p62 gene rescue mice (p62-mRes), which express p62 only in muscle and were derived from p62-knock out mice (p62 KIKI ) using the cre/loxp system. (figshare.com)
  • p62 KIKI mice developed severe NASH when fed an HFD, but the progression of NASH was retarded by p62 gene rescue in muscle, and the expression of Tgf-β1, which encodes a factor that promotes hepatic fibrosis, was reduced. (figshare.com)
  • Ting and colleagues in this study therefore sought to determine whether mice lacking the NLRP12 gene are more susceptible to obesity. (integrativepractitioner.com)
  • The scientists fed mice that lacked the NLRP12 gene and ordinary mice a high-fat diet for several months. (integrativepractitioner.com)
  • Gene expression studies performed on PPARα null mice have shed light into a variety of genes regulated by PPARα. (wur.nl)
  • The role of PPARα in gene regulation in mouse liver is well characterized. (wur.nl)
  • We generated mice that possess a heterozygous deletion of the PTG gene. (jci.org)
  • Moreover, since more than half of Plasmodium genes are required for normal asexual blood-stage reproduction, and cannot be targeted using knockout methods, we discuss how CRISPR/Cas9 could be used to scale up conditional gene knockdown approaches to systematically assign function to essential genes. (portlandpress.com)
  • A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
  • In addition, adipocyte basal lipolysis (55 %) and in vivo lipolysis index (30 %) were significantly decreased in apoCIII mice. (biomedcentral.com)
  • OBJECTIVE- FFAR1/GPR40 is a G-protein-coupled receptor expressed predominantly in pancreatic islets mediating free fatty acid-induced insulin secretion. (diabetesjournals.org)
  • A more recent report suggested that although FFAR1 was necessary for fatty acid-induced insulin secretion in vivo, deletion of FFAR1 did not protect pancreatic islets against fatty acid-induced islet dysfunction. (diabetesjournals.org)
  • Our results showed that under HFD condition, SCD4-/- mice had lower adiposity, improved insulin resistance index and preserved heart structure compared to control mice. (edu.pl)
  • We have previously observed an increased adiposity in response to a high fat diet (HFD) in mice overexpressing apoCIII. (biomedcentral.com)
  • Transcriptomic analysis of liver biopsies in patients with NASH revealed that NASH progression is associated with rewiring of metabolic pathways, including upregulation of de novo lipid/cholesterol synthesis and fatty acid remodelling. (nih.gov)
  • The protein expression of mechanistic target of rapamycin, which promotes muscle protein synthesis, and GLUT4, a glucose transporter in skeletal muscle, were higher in the p62-mRes mice. (figshare.com)
  • In contrast, pathways connected to DNA synthesis were downregulated by fenofibrate in chimeric mice with hepatocyte humanized livers yet upregulated by fenofibrate in normal mouse livers. (wur.nl)
  • These mice have reduced glycogen stores in adipose tissue, liver, heart, and skeletal muscle, corresponding with decreased glycogen synthase activity and glycogen synthesis rate. (jci.org)
  • NMR studies have tracked a correlation between OxPhos and insulin resistance in skeletal muscle. (bruker.com)
  • Initial metabolic analysis of the mice who had OxPhos deficient skeletal muscle at 8 weeks revealed a surprise. (bruker.com)
  • Introduction: Obesity is a risk factor for many diseases because it leads to a reduction in skeletal muscle mass and promotes insulin resistance. (figshare.com)
  • In the present study, we aimed to determine the effects of muscle p62 on skeletal muscle mass, muscle strength, insulin resistance, and NASH pathology. (figshare.com)
  • Limb skeletal muscle mass, grip strength, and the cross-sectional area of muscle fibers were higher in p62-mRes mice than in p62 KIKI . (figshare.com)
  • The research contained in this thesis first sought to improve our current knowledge on the transcriptional regulation by peroxisome proliferator-activated receptors (PPAR)-α activation on human liver in vivo using a novel humanized-liver mouse model. (wur.nl)
  • Recent studies have revealed numerous potential regulators of adult islet function, many of which are not expressed in mouse or immature human β-like cells. (einsteinmed.edu)
  • Importantly, these are unique to human beta cell/islet maturation: they are not expressed in adult mouse islet cells or β-like cells from renewable sources. (einsteinmed.edu)
  • We are now fully characterizing this protein and evaluating its utility as a drug target for diabetes, dyslipidemia, and fatty liver disease. (yale.edu)
  • Methods: Female wild-type (SOD +/+ ) and Cu/Zn-SOD knockout (SOD -/- ) mice were pair-fed ethanol and control liquid diets for 24 days, after which liver injury was assessed. (elsevierpure.com)
  • 2005) have shown that deletion of mitochondrial flavoprotein apoptosis inducing factor (AIF) in mice leads to progressive OxPhos dysfunction. (bruker.com)
  • A novel model to study in vivo PPARα activation in human liver is a chimeric mouse carrying human liver cells. (wur.nl)
  • Emotional contagion in mouse model of Autism Spectrum Disorder. (edu.pl)
  • Aim of my PhD project was to assess the empathic abilities and the activity pattern within the amygdala and prefrontal cortex of Fmr1KO(FVB) mice (both males and females) - commonly used mouse model of ASD. (edu.pl)
  • To understand the reciprocal inter-organ regulatory routes, we reported the construction of liver/adipose double GHR knockout mouse model (DKO). (tus.ac.jp)
  • Splicing-directed therapy in a new mouse model of human accelerated aging. (unil.ch)
  • Thus, the ADGAT DKO mice provide a fascinating new model to study the coupling of metabolic energy storage to energy expenditure. (elifesciences.org)
  • We expected to generate a mouse model similar to those of classic lipodystrophy due to defects of TG storage in adipose tissue. (elifesciences.org)
  • [ 2 ] The patients, who cycled at a constant workload corresponding to 70% of their maximum oxygen consumption, received either nicotinic acid or 20% Intralipid infusion, which, respectively, reduced or increased free fatty acid availability. (medscape.com)
  • The NLRP12-knockout mice ate and drank no more than their healthy cousins but accumulated significantly more fat and became heavier. (integrativepractitioner.com)
  • RESULTS- Insulin secretion was reduced by ∼50% in Ffar1 −/− mice, confirming that FFAR1 contributes significantly to fatty acid stimulation of insulin secretion in vivo. (diabetesjournals.org)
  • Unexpectedly, ethanol feeding significantly elevated total and mitochondrial levels of glutathione in SOD knockout mice compared with wild-type mice. (elsevierpure.com)
  • NPC1L1 −/− mice significantly had fewer tumors than wild-type. (biomedcentral.com)
  • The ratio of malignant/tumor in NPC1L1 −/− mice was significantly lower than in wild-type 20 weeks after AOM-DSS treatment. (biomedcentral.com)
  • ND-fed male B-Atgl-KO mice showed decreased insulinaemia and glucose-induced insulin secretion (GSIS) in vivo. (omicsdi.org)
  • Major gaps exist in our knowledge of the molecular mechanisms underlying insulin resistance and type 2 diabetes. (grantome.com)
  • Major gaps exist in our knowledge of the molecular mechanisms underlying insulin resistance and type 2 diabetes, limiting our ability to develop highly effective and safe therapies. (grantome.com)
  • Here, we examined the potential mechanisms involved in this exacerbated response of apoCIII mice to the HFD. (biomedcentral.com)
  • Obese LFD-IL-1RI(-/-) mice exhibited preserved metabolic health. (elsevierpure.com)
  • Consequently, the role of hepatic Reptin in the pathogenesis of insulin resistance (IR) and non-alcoholic fatty liver disease consecutive to a high-fat diet was investigated. (nih.gov)
  • Sedentary behavior (SB) triggers an inability to adjust substrate use to substrate availability (low metabolic flexibility, MF), which may precede glucose intolerance in the pathogenesis of insulin resistance. (mountainscholar.org)
  • Insuf﫿cient adipose lipid storage is asso- ciated with many pathological conditions including hyperlipidemia, insulin resistance, and type 2 diabetes. (deepdyve.com)
  • Nonetheless, IL-1 receptor I-deficient (IL-1RI(-/-)) mice develop mature-onset obesity despite consuming a low-fat diet (LFD). (elsevierpure.com)
  • Deletion of Lkb1 in adult mice results in body weight reduction and lethality. (omicsdi.org)
  • At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
  • Results: Ethanol-fed SOD -/- mice had 4-fold higher blood ethanol, 2.8-fold higher alanine aminotransferase levels, 20% higher liver weight, a 1.4-fold rise in hepatic protein levels, and 35 to 70% higher levels of lipid peroxides than corresponding wild-type mice. (elsevierpure.com)
  • Ethanol administration caused no significant change in proteasome activity, but caused lysosomal leakage in livers of SOD -/- mice but not in wild-type mice. (elsevierpure.com)
  • Additionally, while ethanol administration induced cytochrome P450 2E1 (CYP2E1) activity in wild-type mice, it caused no such induction in SOD -/- mice. (elsevierpure.com)
  • Wild-type mice and NPC1L1 −/− (NPC1L1 knockout) mice were treated with azoxymethane (AOM)-dextran sodium sulfate (DSS) to induce colitis-associated colorectal tumorigenesis. (biomedcentral.com)
  • NPC1L1 was highly expressed in the small intestine of wild-type mice but its expression was undetectable in colorectal mucous membranes or tumors in either group. (biomedcentral.com)
  • We found that SET domain bifurcated 1 Since no difference in food intake was observed between adqcKO f/f (Setdb1) was a SUMOylated protein and that Senp2 de-SUMOylated Senp2 and Senp2 mice fedeitherwithNCD or HFD and regulated Setdb1 action in trimethylation at histone 3 lysine 9 (Supplementary Figure S2A). (deepdyve.com)
  • Following Reptin invalidation, mice displayed decreased body and fat mass, hypoglycaemia and hypolipidaemia. (nih.gov)
  • In this research, we performed transcriptomics analysis on the effect of fenofibrate in mice with hepatocyte-humanized livers and compared the results with other relevant transcriptomics datasets. (wur.nl)
  • 2007) investigate whether defects in mitochondrial respiration cause T2DM and obesity using AIF to generate models of OxPhos deficiency in mice. (bruker.com)
  • We recently identified a role for ABCC1, a transmembrane drug-resistance transporter, as a GC exporter which limits intracellular GC concentrations and action in adipose tissue. (endocrine-abstracts.org)
  • Fatty acid binding proteins (FABP) are small (approximately 13-14 kDa) intracellular proteins with a high degree of tissue specificity. (thermofisher.com)
  • However, expression of macrophage/inflammatory markers and fibrosis were not different between HILPDA knockout and floxed mice. (wur.nl)
  • In contrast, our new data indicate DNL in adipose tissue is metabolically beneficial since it promotes insulin sensitivity an protects against high fat diet-induced insulin resistance. (grantome.com)
  • We use modern molecular biology, cellular biology and RNA sequencing approaches, in addition of metabolically phenotyping our conditional knockout models. (unil.ch)
  • However, ADGAT DKO mice were unexpectedly otherwise metabolically healthy and did not accumulate TGs ectopically or develop associated metabolic perturbations, even when fed a high-fat diet. (elifesciences.org)
  • Basically, they made the mice very inefficient consumers of fuel, which prevented them from gaining weight on a high fat diet or developing insulin resistance. (bruker.com)
  • To address this issue, we used SCD4 knock-out (SCD4-/-) mice fed a high-fat diet (HFD) to induce obesity. (edu.pl)
  • With this apparent contradiction, the present study evaluated whether IL-1RI(-/-) mice were protected against long-term (6 mo) high-fat diet (HFD)-induced IR. (elsevierpure.com)