Factors protein factor. Medical search. Frequent questions

The APCR assay uses snake venoms to activate factor V and prothrombin, thus triggering the lower portion of the clotting pathway and eliminating interference by factor VIII and by lupus anticoagulants (in the absence of phospholipid complexes). (medscape.com)
The assay is highly sensitive and specific for factor V Leiden, with excellent discrimination between normal individuals and factor V Leiden heterozygous carriers. (medscape.com)
NEW YORK (GenomeWeb) - A new microfluidics-driven DNA-protein binding assay has its creators dreaming of launching a startup to cash in on several potential applications. (genomeweb.com)
The assay could help accelerate efforts to map where transcription factors bind the genomes of humans and model organisms, Deplancke said. (genomeweb.com)
Bacteria often can't support over-expression needed to get workable quantities of protein for an assay, and in vitro expression often can't synthesize a fully functional transcription factor. (genomeweb.com)
The second aim of my work was to identify the interacting proteins that could influence the functionality of TWIST using yeast-two-hybrid assay. (uni-marburg.de)
The direct interaction of SEF2 with TWIST was verified in a yeast mating assay and then confirmed in an in vivo NLS-rescue assay using U2-OS cells, showing that SEF2 forms a heterodimer with TWIST protein and co-localized into the nucleus. (uni-marburg.de)
The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
We previously identified two cytosolic proteins that reconstitute this step in a permeabilized cell assay: the 54/56-kD NLS receptor and p97. (rupress.org)
The activated protein C resistance assay is a screening test for the presence of factor V Leiden. (msdmanuals.com)

One of the great missing pieces in our understanding of gene expression is how transcription factors regulate and control it in an orchestrated fashion. (genomeweb.com)
I wanted to determine if the TWIST protein or its conserved motifs interact with other regulatory proteins to help to regulate the TWIST transcriptional activity. (uni-marburg.de)
Existing studies have shown that there is crossover between Renin-Angiotensin-Aldosterone (RAAS) signaling pathway and TGF-β pathway, but little is known about whether ARB drug losartan can block Angiotensin-II (Ang-II) signaling pathway, inhibit the activation of Transforming Growth Factor- Beta (TGF-β) pathway, and regulate vascular remodeling. (ijpsonline.com)
Transcription factors help to regulate gene expression-turning genes on or off and dialing up or down their level of activity-often in partnership with the proteins that they bind. (mit.edu)
The C9orf72 protein is predicted to be a differentially expressed in normal and neoplastic cells domain protein implying that C9orf72 functions as a guanine nucleotide exchange factor (GEF) to regulate specific Rab GTPases. (bath.ac.uk)

Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. (curehunter.com)
Additionally, we find that the ESC genome is organized in such a way as to juxtapose regions of DNA that show similar regulation by demonstrating that spatially associated regions in the genome are associated with the same gene regulatory factors. (ca.gov)
Together, our data highlight a pluripotency-specific genome organization in which pluripotency factors such as Nanog and H3K27me3 occupy distinct nuclear spaces and reveal a role for cell-type-specific gene-regulatory networks in genome organization. (ca.gov)
They anchor themselves and their partner proteins to DNA at binding sites in genetic regulatory sequences, bringing together the components that are needed to make gene expression happen. (mit.edu)

The other protein inhibits the opportunity for this to occur," said John Ladbury, Ph.D., professor of Biochemistry and Molecular Biology. (mdanderson.org)
This gene encodes for a bHLH transcription factor, which inhibits osteogenic differentiation by transc. (uni-marburg.de)
Losartan has protective effect on aortic wall of rats with aortic dissection, and the mechanism may be through inhibiting transforming growth factor-beta 1 pathway, which activates downstream phosphoinositide 3-kinase/protein kinase B pathway related proteins, and then inhibits the expansion and progression of aortic dissection. (ijpsonline.com)

The second screening of the yeast clones suggested some promising candidates protein as interacting partners with the NSEEE motif such as ETV5, SURF4, Spastin, Metalloproteinase 2, and ALR-like protein mRNA. (uni-marburg.de)
We also observed a clear increase in CTGF mRNA and protein expression levels in HBV-positive HCC human tissue samples in comparison with the HBV-negative controls, indicating a possible negatively associated relationship between miR-18a and CTGF. (medscimonit.com)
Compared with the BSA controls, the RGC-5 cells incubated with AGE-BSA showed a dose- and time-dependent increase in VEGF-A mRNA and VEGF-A protein secretion in the supernatant, with the highest levels achieved at 24 h. (molvis.org)

Serum protein electrophoresis (SPEP) is used to quantify the serum monoclonal component or M-protein, for diagnosis and monitoring of monoclonal gammopathies. (degruyter.com)
Using therapeutic monoclonal antibody-spiked sera and a pooled beta-migrating M-protein, we aimed to assess SPEP limitations and variability across 16 laboratories in three continents. (degruyter.com)
Founded on the secretion of a monoclonal immunoglobulin (M-protein), plasma cell proliferative disorders are classified as monoclonal gammopathies and include multiple myeloma (MM), Waldenström's macroglobulinemia (WM), amyloidosis (AL), light chain deposition disease (LCDD), POEMS syndrome, and premalignant diseases such as monoclonal gammopathy of undetermined significance (MGUS) and smoldering multiple myeloma (SMM). (degruyter.com)
Due to the wide range of disease presentations, identification of the M-protein by serum protein electrophoresis (SPEP) may be the first clue to the diagnosis of a monoclonal gammopathy followed by subsequent isotyping via immunofixation (IFE) or immunosubtraction (ISUB). (degruyter.com)
In monoclonal gammopathies, M-proteins are visualized as a restricted area of migration in the electrophoretic pattern which can be gated and quantified [ 4 ]. (degruyter.com)
A monoclonal antibody to p97 localizes the protein to the cytoplasm and the nuclear envelope. (rupress.org)

Sequence and characterization of cytoplasmic nuclear protein import factor p97. (rupress.org)
Here we report the expression, purification and biochemical characterization of C9orf72 protein. (bath.ac.uk)

Being only one atom thick and having a unique charge pattern lets these coin-shaped particles grab onto proteins and growth factors that they can then slowly release. (medgadget.com)
While these disks have not been tried in patients, laboratory experiments have shown that they do trap proteins and growth factors and then release them slowly over a period of about a month. (medgadget.com)
These interactions occur outside normal activation of FGFR2 by growth factors, so the protein with the highest concentration levels in the cell wins the contest to bind to FGFR, or fibroblast growth factor receptor 2, Ladbury said. (mdanderson.org)
6% of the cycles benefited from medullary growth factors. (curehunter.com)

In terms of predicting where in the genome the transcription factors bind, the models we're able to derive seem to be, in general, actually better than the other assays. (genomeweb.com)
That turned out to be really useful in order to express transcription factors that other assays have trouble working with. (genomeweb.com)

Selective microfluidics-based ligand enrichment followed by sequencing, or SMiLE-seq, combines antibody arrays, mechanical trapping, and next-generation sequencing readouts to provide a new platform for characterizing DNA-protein interactions. (genomeweb.com)
Additionally, we identified two further levels of genome organization in ESCs characterized by colocalization of regions with high pluripotency factor occupancy and strong enrichment for Polycomb proteins/H3K27me3, respectively. (ca.gov)

The present study was thus undertaken to determine how TWIST1 gene mutations affect protein function. (uni-marburg.de)
All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)
Mutations of factor V make it resistant to its normal cleavage and inactivation by activated protein C, and they also predispose to venous thrombosis. (msdmanuals.com)
Any of several mutations to factor V make it resistant to inactivation by APC, increasing the tendency for thrombosis. (msdmanuals.com)
Factor V Leiden is the most common of these mutations. (msdmanuals.com)

That year, the two published a paper in Science describing a method to trap low-affinity DNA-protein interactions, specifically those of transcription factors. (genomeweb.com)
By physically restraining bound molecules against an antibody array, they capture both transient interactions, which yeast two-hybrid or tandem mass spectrometry methods don't, and weak interactions, which are usually obliterated by the wash in protein-DNA microarrays. (genomeweb.com)
For SMiLE-seq, what the button captures is interactions between GFP-labeled transcription factors and a library of oligonucleotides. (genomeweb.com)
The transcription factors bind to any number of DNA motifs in the library, those interactions are trapped, and then the readout matches up each motif with the transcription factor via the barcode. (genomeweb.com)
Underlining the independence of these networks and their functional relevance for genome organization, loss of the Polycomb protein Eed diminishes interactions between Polycomb-regulated regions without altering overarching chromosome conformation. (ca.gov)
The clues have been there all along, but I think earlier work was so focused on the DNA and protein interactions that they didn't consider RNA. (mit.edu)

Patients with abnormal APCR ratios should have genetic testing to confirm factor V Leiden mutation status. (medscape.com)
Hypertension, physical trauma, smoking, previous cardiac surgery, male sex and genetic diseases are risk factors for AD[ 2 ]. (ijpsonline.com)
Numerous genetic, acquired, and environmental factors can tip. (msdmanuals.com)
Understanding of the significant genetic risk factors for Cholangiocarcinoma (CC) remains limited. (bvsalud.org)

To investigate the effect of losartan on vascular remodeling and transforming growth factor-beta and phosphoinositide 3-kinase/protein kinase B pathway. (ijpsonline.com)
Hematoxylin and eosin staining was used to measure the thickness and diameter ratio of the aortic media of mice in each group, Masson staining was used to observe the content of collagen fibers in the aorta of mice in each group, elastic fibers in the aorta of mice in each group were stained, and transforming growth factor-beta and phosphoinositide 3-kinase/protein kinase B pathway protein expression. (ijpsonline.com)
The expression of HMGB1, c-Jun N-terminal kinase (JNK), extracellular-signal-regulated kinase (ERK), and p38 mitogen-activated protein kinase (p38 MAPK) was assessed with immunofluorescence and western blot analysis. (molvis.org)

NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. (hmdb.ca)

A few papers, including one from Young's lab, had previously identified individual transcription factors as being able to bind RNA, but researchers thought that this was a quirk of the specific transcription factors. (mit.edu)

Heterozygous carriers of factor V Leiden typically have APCR ratios in the range of 1.5 -1.8. (medscape.com)
The risk of recurrent venous thromboembolism in heterozygous carriers of factor V Leiden and a first spontaneous venous thromboembolism. (msdmanuals.com)

The aim of my work is to characterize functional domains in TWIST protein, and to determine the interacting partner for TWIST and its motifs particularly NSEEE and WR. (uni-marburg.de)
Furthermore, two more highly conserved TWIST motifs NSEEE and WR were analyzed individually to find out their interacting proteins and their role in regulating the TWIST1 transcriptional activity. (uni-marburg.de)

Insulin-like growth factor-binding proteins (IGFBPs) control bioavailability, activity, and distribution of insulin-like growth factor (IGF)1 and -2 through high-affinity IGFBP/IGF complexes. (rcsb.org)
Nuclear location sequence-mediated binding of karyophilic proteins to the nuclear pore complexes is one of the earliest steps in nuclear protein import. (rupress.org)

Factor V Leiden is found in 30-50% of patients with recurrent venous thromboembolism (VTE) and is the most common hereditary cause of thrombosis. (medscape.com)
Karmacharya P, Aryal MR, Donato A. Mesenteric vein thrombosis in a patient heterozygous for factor V Leiden and G20210A prothrombin genotypes. (medscape.com)
Spontaneous central vein thrombosis in a patient with activated protein C resistance and dengue infection: An association or causation? (medscape.com)

The researchers found that RNA binding keeps transcription factors near their DNA binding sites for longer, helping to fine tune gene expression. (mit.edu)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

There is greater imprecision and loss of accuracy at lower M-protein concentrations. (degruyter.com)
This study suggests that quantifying exceedingly low concentrations of M-proteins, although possible, may not yield adequate accuracy and precision between laboratories. (degruyter.com)

Glycation, the result of a protein or lipid molecule bonding with sugar molecules, is a consequence of the aging process. (molvis.org)

Factor V is a key component in blood coagulation systems, where deficiencies can inhibit thrombin generation and affect hemostasis. (sigmaaldrich.com)

Immunodepletion of p97 renders the cytosol inactive for import and identifies at least three other cytosolic proteins that interact with p97. (rupress.org)

High-mobility group box 1 protein (HMGB1) has been reported to be a potent proangiogenic factor induced by inflammatory stress. (molvis.org)

Quantifying the relative concentration of these two proteins in a patient's tumor, Ladbury said, might be developed into reliable markers for gauging the likelihood that the cancer will spread and guide treatment decisions. (mdanderson.org)
Plasma tumor necrosis factor-α and C-reactive protein as biomarker for survival in head and neck squamous cell carcinoma. (diva-portal.org)
In this study, a possible role of plasma interleukin-2 (IL-2), interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) as biomarkers for survival of HNSCC patients was investigated. (diva-portal.org)
The most important prognostic factors are tumor size, local spread and distant metastases, tumor node metastasis (TNM) staging. (diva-portal.org)
Higher levels of C-reactive protein (CRP) and tumor necrosis factor alpha (TNF-α) were detected in plasma of the patients compared with the levels in the controls. (diva-portal.org)

Activated protein C (APC) is the enzymatically active form of protein C after proteolytic cleavage by thrombomodulin-bound thrombin. (medscape.com)
In the vast majority of cases, the patient with APCR carries the Leiden variant of factor V, in which the APC cleavage site on factor V is altered. (medscape.com)
In patients with homozygous or heterozygous factor V Leiden resistance , the prolongation of the clotting time is significantly reduced because their factor V is resistant to cleavage by activated protein C. (msdmanuals.com)

Advances in molecular biology have allowed research groups to identify numerous virulence factors and to explore their roles in the progression of S. iniae infection. (cdc.gov)

An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. (degruyter.com)
Protein repletion after hip fracture was associated with increased serum levels of insulin-like growth factor-I, attenuation of proximal femur bone loss, and shorter stay in rehabilitation hospitals. (acpjournals.org)

They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. (curehunter.com)

Head and neck cancer (HNC) is a collective name for heterogeneous tumors located in the head and neck regions for which smoking, alcohol and human papillomavirus (HPV) are documented risk factors. (diva-portal.org)

Lately, attempts at protein and growth factor therapies for cartilage repair have involved delivering large doses to patients, as these substances don't stay around long enough for smaller doses to work. (medgadget.com)
In paper I, a possible role of plasma cytokines or proteins associated with immune response or inflammation, as biomarkers for the survival of HNC patients was investigated. (diva-portal.org)
To investigate whether oral protein supplements benefit bone metabolism in patients with recent hip fracture. (acpjournals.org)
Older patients who received unpurified plasma‐derived clotting factor concentrates may have signs and symptoms of infectious disease (eg, hepatitis, HIV infection). (medscape.com)

Hemophilia B, or Christmas disease, is an inherited, recessive disorder that involves deficiency of functional coagulation factor IX (FIX) in plasma. (medscape.com)

As the background increases, imprecision increases and accuracy decreases leading to overestimation of M-protein quantitation especially evident in hypergamma samples, and more prominent with PD. (degruyter.com)

Deplancke himself wondered whether this was a scalable technology, or whether it would only be useful to labs interested in transcription factor function. (genomeweb.com)

The hemostatic pathway: role of factor IX. (medscape.com)

This synergistic effect is consistent with the observation that combined K38R (NLS1) and K76R (NLS2) mutants dramatically reduced nuclear localization, further suggesting that both NLS1 and NLS2 work together in regulating nuclear localization of TWIST protein. (uni-marburg.de)
Recombinant p97 binds zinc and a bound metal ion is required for the nuclear envelope binding activity of the protein. (rupress.org)

In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. (molvis.org)

The normal reference value for the activated protein C resistance (APCR) ratio is greater than 2.1. (medscape.com)
APC resistance (APCR) is a hypercoagulability disorder in which factor V cannot be inactivated by APC. (medscape.com)

Ladbury is senior author of a paper published Sunday online at Nature Structural & Molecular Biology that describes the competition, identifies Plγcl's role and its relationship to the metastasis-blocking growth factor receptor bound protein 2 (Grb2). (mdanderson.org)
In performing this role, Grb2 blocks the binding of other proteins such as Plcγ1. (mdanderson.org)
This study was designed to improve our understanding of the role of miR-18a and its target (connective tissue growth factor (CTGF), which are mediators in HBX-induced hepatocellular carcinoma (HCC). (medscimonit.com)
Long-Range Chromatin Contacts in Embryonic Stem Cells Reveal a Role for Pluripotency Factors and Polycomb Proteins in Genome Organization. (ca.gov)

An important natural anticoagulant, APC inactivates factors Va and VIIa. (medscape.com)

When transcription factors had their ARMs mutated so they couldn't bind RNA, those transcription factors were less effective in finding their target sites, remaining at those sites and regulating genes. (mit.edu)

Factor V, a glycoprotein is usually seen in the alpha granules of platelets and in plasma. (sigmaaldrich.com)
One unit is equivalent to the Factor V activity in 1.0 mL of normal human plasma at pH 7.4 at 37 °C. (sigmaaldrich.com)
Activated protein C is added to a 5-fold dilution of patient plasma, and the coagulation time is measured. (msdmanuals.com)

Homozygotes for factor V Leiden usually have APRC ratios of less than 1.5. (medscape.com)
The factor V Leiden mutation is not uncommon, with the heterozygous carrier state occurring in up to 5% of the US population. (medscape.com)
Ethnic distribution of factor V Leiden in 4047 men and women. (msdmanuals.com)
Confirmation of factor V Leiden is then done by DNA-based testing for the mutation. (msdmanuals.com)

Recently, we isolated a novel negative regulator of epidermal growth factor receptor (EGFR), termed EGFR-related protein (ERRP), whose expression attenuates EGFR activation. (aacrjournals.org)

Aberrant promoter methylation of insulin-like growth factor binding protein-3 gene in human cancers. (nih.gov)
Here, we examined the methylation status of IGFBP-3 to correlate to clinicopathological factors in human cancers. (nih.gov)

Assessment of the effects of occupational and environmental factors on all faster and slower large myelinated nerve fibers: A study of the distribution of nerve conduction velocities. (cdc.gov)

On the other end they have a region that can bind to proteins. (mit.edu)
In a paper published in Molecular Cell on July 3 , Young and postdocs Ozgur Oksuz and Jonathan Henninger reveal that along with DNA and protein, many transcription factors can also bind RNA. (mit.edu)
Further testing confirmed that many transcription factors do in fact use their ARMs to bind RNA. (mit.edu)

The distinctive presence of both Rab- and Rho-GTPase GEF activities suggests that C9orf72 may function as a dual exchange factor coupling physiological functions such as cytoskeleton modulation and autophagy with endocytosis. (bath.ac.uk)

Next, the researchers tested to see if RNA binding affected the transcription factors' function. (mit.edu)

Factor Va is the activated form of factor V and the cofactor of Xa. (sigmaaldrich.com)

This rethinking of how transcription factors work may lead to a better understanding of gene regulation, and may provide new targets for RNA-based therapeutics. (mit.edu)

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