• The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
  • During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
  • The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • The GC-MS analysis of the methanolic extract of BG revealed 23 compounds which are reported to possess different biological activities. (biomedcentral.com)
  • Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
  • In 1969, Stähelin made the key decision to include a test system for immunosuppression into the general screening program at Sandoz for compounds and extracts. (wikipedia.org)
  • Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
  • The inclusion of other assays and compounds will most likely expand the types of activities that can be reliably detected. (holocausto.online)
  • Traditionally, the in vitro toxicity assays that have been used to identify molecules that carry drug-related DILI risk factors include two-dimensional (2D) cell systems and other standard preclinical model… The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. (holocausto.online)
  • Our researchers are now extracting bacterial DNA from soil and marine habitats, cloning large fragments into, for example, bacterial artificial chromosomes, expressing in a host bacterium and screening the library for new compounds. (microbialtec.com)
  • Compounds of interest include not only classical "drug-like" organic small molecules, but also peptides, proteins, and other bioactive chemical classes. (cancer.gov)
  • The bioassay-guided separation of the extract provided ten active compounds, including three new N-methyladenine-containing diterpenoids, agelasines W-Y ( 1 - 3 ), a new bromopyrrole alkaloid, N(1)-methylisoageliferin ( 4 ), and six known ageliferin derivatives ( 5 - 10 ). (cancer.gov)
  • This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • Prediction of Chemical Carcinogenicity in Rodents from in Vitro Genetic Toxicity Assays R AYMOND W. TENNANT, BARRY H. MARGOLIN, MICHAEL D. SHELBY, E RROL Z … As an in vivo toxicity endpoint, the acute lung inflammogenicity in a rat instillation model was compared with the in vitro toxicity endpoints comprising cytotoxicity, pro-inflammatory cytokine expression, or haemolytic potential. (holocausto.online)
  • We conduct cytotoxicity estimation using a panel of cell proliferation and viability tests, which can be sorted into four types by different endpoints: Functional assays: assessment of ATP, ADP, ATP/ADP ratios. (holocausto.online)
  • Kroll A(1), Pillukat MH, Hahn D, Schnekenburger J. The in vitro assays covered baseline toxicity in bacteria, oxidative stress, cytotoxicity in mammalian cells and yeast, estrogenicity, and anti-androgenicity. (holocausto.online)
  • In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. (holocausto.online)
  • The search for targeted drugs for cancer treatment includes consideration of natural products, which may provide new opportunities for antitumor cytotoxicity as single agents or in combination therapy. (cancer.gov)
  • L. egeregia was regarded as the prototype of the anticancer species due to its profound flavonoid concentration (85.40 µg/mL) and cytotoxicity (9.46 µg/mL) compared to other extracts. (who.int)
  • In vitro toxicity assays are used to determine the potential of a new pharmaceutical, agrochemical, food additive, or other chemical product to be hazardous to humans.In vitro studies, which are performed on cultured bacterial or mammalian cells, can be used as an initial screen to avoid the unnecessary use of animals in determining which candidates should move forward for further safety testing. (holocausto.online)
  • The application of High Content Screening for in vitro toxicity testing is a relatively new approach in the preclinical research phase of drug development. (holocausto.online)
  • In regulatory testing situation these in vitro toxicity assays must be reliable, because from these tests it is only possible to generalize the data to … First Published 1995. (holocausto.online)
  • While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss. (holocausto.online)
  • Considering the ethical issues and the cost of in-vivo animal tests, the pharmaceutical industry now relies more on in-vitro methods for toxicity testing in the drug development phase. (holocausto.online)
  • H … In vitro assays covered baseline toxicity, oxidative stress responses, and estrogenic and antiandrogenic activity. (holocausto.online)
  • The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. (holocausto.online)
  • Compared with the classic 2D cell cultures, the use of spheroids in the antitumoral drugs selection process imitates in a better way the in vivo physiology and the obtained results in vitro have a greater possibility of success. (medinadiscovery.com)
  • In vitro Analysis of Antioxidant, Antimicrobial and Antiproliferative Activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata Extracts. (google.com)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
  • However, determining the potential for clinically significant drug interactions with milk thistle products has been complicated by inconsistencies between in vitro and in vivo study results. (aspetjournals.org)
  • The algorithm for assessing radio protective potential of plant extracts and natural products was assessed via some target in vitro and in vivo tests and clinical trials. (europa.eu)
  • 2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)
  • The methanolic and ethyl acetate extracts of the plant also showed remarkable anti-inflammatory activity where methanolic extracts had the lowest EC50. (biomedcentral.com)
  • It was found that methanolic extracts elicited higher total phenolic content, higher percentage scavenging activity of nitric oxide, hydrogen peroxide, hydroxyl radicals, free radical scavenging (DPPH), FRAP (ferric reducing ability plasma) ability and reducing power. (google.com)
  • Different concentrations of crude methanolic extracts of seaweeds showed potential antimicrobial activity by well diffusion method. (google.com)
  • The methanolic extracts of seaweeds Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata possess high total phenolic content and shows a good free radical scavenging activity and hence are proven to have better antioxidant activity and they might be good candidates for further investigations in order to develop potential anticancer drugs. (google.com)
  • The extract did not induce either lethality in the brine shrimp (Artemia salina Leach) bioassay or antimicrobial activity. (researchgate.net)
  • Brine Shrimp Lethality Bioassay (BSLB) can provide an indication of possible cytotoxic principles in plant extract [ 8 ]. (biomedcentral.com)
  • The use of nanoparticle (NP) delivery systems in cancer treatment has received significant interest, however use of such systems in delivery of cytotoxic chemotherapy agents can be limited by low encapsulation efficiency and burst release of the cytotoxin, as well issues with throughput and reproducibility during the fabrication of drug-loaded NPs. (bvsalud.org)
  • Moreover, the algorithm was able to distinguish cytotoxic vs. cytostatic drug effects and drug-resistant clones that will give rise to later relapse. (technologynetworks.com)
  • N. A. A. Ali, W. D. Julich, C. Kusnick and U. Lindequist, "Screening of Yemeni Medicinal Plants for Antibacterial and Cytotoxic Activities," Journal of Ethnopharmaco- logy, Vol. 74, No. 2, 2001, pp. 173-179. (scirp.org)
  • Additionally, the algorithm was able to distinguish between cytotoxic and cytostatic effects of drugs and drug-resistant clones that will cause subsequent relapse. (genericviabuy.com)
  • Mammalian genotoxicity assay reveals if the compound causes abnormalities in chromosome distribution (aneugenity) or chromosome breaks (clastogenity) during cell division. (holocausto.online)
  • N- trans -Feruloyloctopamine (FO), a natural compound, was reported in our previous study to inhibit a tumor cell malignant phenotype by AKT- and EMT-related signals and might be used as a promising drug for HCC treatment. (hindawi.com)
  • Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
  • Characterization and anti-tumor activity of alkali-extracted polysaccharide from Enteromorpha intestinalis. (google.com)
  • J. R. Zgoda and J. R. Porter, "A Convenient Microdilution Method for Screening Natural Products against Bacteria and Fungi," Pharmaceutical Research, Vol. 39, No. 3, 2001, pp. 211-225. (scirp.org)
  • Table 1 Some FDA-approved drugs from bacteria. (microbialtec.com)
  • Whipple pointed out the existence of great numbers of peculiar rod-shaped bacteria found in extracts of lymph node tissue and lamina propria of the intestine. (medscape.com)
  • Carmen Ramos presented our Natural Products antitumor screening in 3D cell cultures, the ultimate tool designed by MEDINA for the identification of new bioactive Natural Products through a high automated process. (medinadiscovery.com)
  • Bioactive components were extracted by using various solvents. (google.com)
  • Within natural diffusion limit of vascularized tissue, tumor-derived MOS allowed sufficient penetration by therapeutic immune T-cells such as CAR-T, enabling a novel T cell potency assay to assess tumor killing by the engineered T-cells. (technologynetworks.com)
  • Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • When loaded with immune complexes (IC), consisting of tumor antigens bound to antitumor antibody, BMDC induce powerful antitumor immunity in mice. (stanford.edu)
  • MOS could be further integrated with deep-learning imaging analysis for rapid drug testing of small and heterogeneous clinical tumor biopsies. (technologynetworks.com)
  • Increasing amounts of evidence indicate that autophagy is induced by a number of stressors in tumor cells, such as starvation, growth factor deprivation, hypoxia, damage stimulation and therapeutic drugs, and is an important survival mechanism in response to cellular stress ( 9 ). (spandidos-publications.com)
  • All the extracts showed high TPC and TFC along with the stronger correlation with the antioxidant activity of the extracts suggesting phenolics and flavonoids contents of the extract might be attributed to showing antioxidant activity. (biomedcentral.com)
  • NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
  • An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. (holocausto.online)
  • MTT assay was employed to study the anticancer activity against cancer cell lines Hep-G2, MCF7 and normal VERO cell lines. (google.com)
  • Crude methanolic extract of G. corticata had significant anticancer activity followed by E. antenna and E. linza on cancer cell lines Hep-G2, MCF7 and normal VERO cell lines by MTT assay. (google.com)
  • An extract of the coralline demosponge Astrosclera willeyana inhibited the ubiquitin ligase activity of the immunomodulatory protein Cbl-b. (cancer.gov)
  • A number of formulation approaches have been employed to increase the solubility and oral absorption of some herbal extracts and products and subsequently to enhance their bioavailability and therapeutic activity. (europa.eu)
  • N- trans -Feruloyloctopamine (FO) is the extract of garlic skin, which possesses high antioxidant activity and selectively induces cell apoptosis in leukemia tumors [ 6 ]. (hindawi.com)
  • We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
  • Dr. Shen's team has developed emulsion microfluidic technology for creating MOS, tiny, nanoliter-sized basal membrane extract (BME) droplets composed of tissue cell mixtures which can be generated at a rapid pace from an automated device. (technologynetworks.com)
  • Cytology/Membrane leakage assays: LDH, AK test. (holocausto.online)
  • The team of Dr. Shen has developed emulsion microfluidic technology for creating MOS, nanoliter-sized droplets of basement membrane extract (BME) composed of tissue cell mixtures, which can be generated at a rapid rate by an automated device. (genericviabuy.com)
  • While the epitope of most B7-H3 antibodies has not been described, one of the clinically tested antitumor antibodies (8H9) was found to bind to the IgV domain 19 . (nature.com)
  • Pendula Leaf Extracts Against 91 Clinically Important Pathogenic Microbial Strains," Chinese Medicine , Vol. 1 No. 2, 2010, pp. 31-38. (scirp.org)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • S. K. Chakrabarti and B. Mukherjee, "Search for Anticancer Drug from Indian Medicinal Plants," Indian Journal of Medical Research, Vol. 56, No. 4, 1968, pp. 445-455. (scirp.org)
  • The antioxidant capability of the resulting plant extracts against a stable radical DPPH was evaluated and the most of plant extracts showed high efficiency in the DPPH test. (europa.eu)
  • MOS can be further integrated with deep learning image analysis for rapid drug testing of clinical biopsies of small and heterogeneous tumors. (genericviabuy.com)
  • Extracts of hexane, ethyl acetate and methanol were quantitatively estimated for total phenolic contents (TPC) and total flavonoid contents (TFC). (biomedcentral.com)
  • E. compressa extracts alleviated the IgE levels raised against ovalbumin and otherallergens in mice. (google.com)
  • As documented in a recent publication in Stem Cell Reports , their MOS can be used as patient avatars for studies involving direct viral infection, immune cell penetration and high-throughput therapeutic drug screening, something that is not obtainable with conventional patient-derived models. (technologynetworks.com)
  • Some aspects of recent research with natural products directed to produce anti-hypertensive drugs are discussed. (scielo.br)
  • Dr. Francisca Vicente discussed MEDINA's Natural Products Screening Platform using Labcyte's ECHO System at SLAS Europe 2019 in Barcelona at the end of June and pointed out how the miniaturization of high-throughput assays using the Echo 550 Liquid Handler (powered by acoustic droplet ejection) has enabled the maximization of the use of natural product extracts by transferring ultra-low volumes and reducing costs. (medinadiscovery.com)
  • Drugs of natural origin have been classified as (i) original natural products, (ii) products derived or chemically synthesized from natural products or (iii) synthetic products based on natural product structures. (microbialtec.com)
  • The mechanisms of radio protective action of the tested extracts and natural products was analysed. (europa.eu)
  • The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. (scialert.net)
  • Chemical composition of the extracts was analyzed with non-target high-resolution mass spectrometry. (holocausto.online)
  • Amongst all the marine invertebrates, sponges represent the most described group with potential application in medicinal chemistry, representing a valid starting point for new drug leads due to their chemical defense mechanisms. (microbialtec.com)
  • A physico-chemical screening test for chemical carcinogens: The ke test. (cdc.gov)
  • A typical workflow for conventional bioprospecting and drug discovery. (microbialtec.com)
  • Further more, one of the most recent approaches in drug discovery from microorganisms is cocultivation. (microbialtec.com)
  • If you are interested in microbial drug discovery services, please contact us for more details. (microbialtec.com)
  • The Molecular Targets Program (MTP) provides the focus and infrastructure that enables CCR investigators to pursue molecularly targeted drug discovery research by promoting an interdisciplinary, collaborative, team-oriented approach to identifying and validating potential cancer-pertinent targets. (cancer.gov)
  • Also, there are increasing cases of antibiotics resistance among most causative agents of diseases in human beings, which calls for an alternative drug discovery using natural sources. (frontiersin.org)
  • Various anti-oxidants have found to possess properties such as anti-atherosclerotic, antitumor, anti-mutagenic, anti-carcinogenic to name a few selected ones [ 4 ]. (biomedcentral.com)
  • Dr. Shen and his team continue to refine and improve upon the MOS technology and to spotlight its versatility, not only as a physiological model for screening potential personalized treatments, but for disease studies and a variety of other applications as well," said Ali Khademhosseini, Ph.D., TIBI's Director and CEO. (technologynetworks.com)
  • Dr. Shen and his team continue to refine and improve the MOS technology and demonstrate its versatility, not only as a physiological model for screening potential personalized treatments, but also for disease studies and a variety of applications of others," said Ali. (genericviabuy.com)
  • 16 herbal extracts were analyzed for the presence of bio-active constituents and antioxidant potential. (europa.eu)
  • This has led to sudden increase in the number of herbal industries in the drug market. (pharmatutor.org)
  • These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • Using cDNA microarray screens and northern blot analysis, a previous study showed that Beclin 1 is upregulated in liver cancer tissues ( 11 ). (spandidos-publications.com)
  • Here, we report the draft genomes for 45 Enterobacterales clinical isolates, including historical and contemporary drug-resistant organisms, obtained in Pakistan between 1998 and 2016: 5 Serratia, 3 Salmonella, 3 Enterobacter, and 34 Klebsiella. (cdc.gov)
  • Milk thistle ( Silybum marianum ) extracts are widely used as a complementary and alternative treatment of various hepatic conditions and a host of other diseases/disorders. (aspetjournals.org)
  • Dr. Shen's team was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (technologynetworks.com)
  • The team of Dr. Shen was able to create a MOS atlas of human respiratory and digestive tissues from patient autopsies and infect them with SARS-COV-2 viruses, followed by drug screening to identify drugs that block viral infection and replication within those tissues. (genericviabuy.com)
  • Despite that smooth if he has a mean hyperborean , ear infection , urgent throat , or fever , more drug isn't better. (wgc2010.sk)
  • However, the drug targets and the detailed mechanisms have been masked. (hindawi.com)
  • The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
  • Hepatotoxicity (toxic effects on the liver) and cardiotoxicity (toxic effects on the heart) are the most severe problems encountered in drug development and the major reason for drug withdrawals. (holocausto.online)
  • Chemotherapeutic drugs result in not only strong resistance but also more side effects [ 4 ]. (hindawi.com)
  • Endophytes still present themselves as a peculiar source of possible drugs. (frontiersin.org)
  • Extraction and direct contact assays were performed using human epidermal keratinocytes and mouse fibroblasts and mouse epithelial cells. (holocausto.online)
  • Screening with RNA-Seq in Huh7 cells treated with FO revealed that 317 genes were modulated, of which 188 genes were upregulated and 129 genes were downregulated. (hindawi.com)
  • Herein, genome-wide transcriptome profiling (RNA-Seq) was used to screen the differentially expressed genes (DEGs) in FO-treated Huh7 cells, with the aim of uncovering the pharmacologic pattern. (hindawi.com)
  • The aim of the present study was to determine the effect of a standardized milk thistle supplement on major P450 drug-metabolizing enzymes after a 14-day exposure period. (aspetjournals.org)
  • Western blot and Enzyme-linked Immunosorbent Assays (ELISA) were used to identify and investigate the immunoreactivity of FVpE protein. (bvsalud.org)
  • From accessing medicines to intellectual property to drug safety, PhRMA is devoted to advancing public policies that support innovative medical research, improve treatments and yield real results. (wgc2010.sk)
  • Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
  • The phytochemical screening detected the presence of saponins, alkaloids, reducing sugars and coumarins. (researchgate.net)
  • The review refers to 321 plants, the parts utilized, type of extract and whether they are active or not. (scielo.br)
  • The active constituents of milk thistle supplements are believed to be the flavonolignans contained within the extracts. (aspetjournals.org)
  • Fenugreek seeds have been used as an oral insulin substitute, and seed extracts have been reported to lower blood glucose levels. (drugs.com)