• The individual should apply their significant expertise in molecular biology based techniques, along with their understanding such as plasmid cloning, gene editing, AAV vector production, electroporation, qPCR, RT-PCR, nucleic acid extraction and cloning, gel electrophoresis, macromolecule blotting and probing and mouse handling is highly desirable. (fems-microbiology.org)
  • Three partial genomic clones were generated and the sequence analysis revealed that they were identical at both nucleotide and amino acid levels. (scialert.net)
  • Christine formerly managed a biotechnology lab for a major seed company, where she implemented new genomic and biotechnology tools for the breeders and trait conversion teams in order to enhance their understanding of the germplasm at a molecular genomic level. (quarles.com)
  • From genomic libraries, a gene that makes an important biomedical compound can be cloned and expressed as a chemical compound in an artificial system. (noaa.gov)
  • Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. (bvsalud.org)
  • Expertise in the in-depth analysis of gene expression and genome editing using various molecular and cell biology analysis methods such as quantitative PCR and RT-PCR, Next generation sequencing, Southern, Northern, and Western blotting, flow cytometry, and fluorescence microscopy. (fems-microbiology.org)
  • We offer reagents that cover all the workflow needed in a PCR, from DNA extraction to polymerases, buffers, primers, enhancers, PCR kits etc. (isogen-lifescience.com)
  • Ltd. specializes in primer synthesis, extraction columns DNA and RNA & PCR reagents. (biosciregister.com)
  • Severn Biotech Limited manufactures products and services for the molecular biologist. (biosciregister.com)
  • The molecular biologist begins his or her research when samples of marine organisms reach the surface. (noaa.gov)
  • I am a trained molecular biologist, biochemist and microbiologist with experience in different aspects of commercial product development, going from conception to launch. (callaghaninnovation.govt.nz)
  • Acid guanidinium thiocyanate-phenol-chloroform extraction Ethanol precipitation Spin column-based nucleic acid purification Chan, P K S. (wikipedia.org)
  • DNA and RNA extraction/purification (mini and maxi preps, etc. (ziprecruiter.com)
  • The SPINeasy PCR Purification and Gel Extraction Kit is a silica -membrane spin -column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gels. (thomassci.com)
  • Hands-on experience in molecular biology: multichannel pipetting, microplate assay preparation, multi-mode microplate reader, PCR, and nucleic acid purification. (astrixinc.com)
  • As a Senior Scientist in the Proteins and Enzymes team, my main focus is the development of extraction and purification strategies and analysis of high value proteins and enzymes. (callaghaninnovation.govt.nz)
  • The TransLab offers a comprehensive battery of cellular and molecular assays to help guide personalized therapy or support patient monitoring. (childrenshospital.org)
  • Noncompetitive immunoassays are advantageous over competitive assays for the detection of small molecular weight compounds. (cdc.gov)
  • Biotechnology Unit at Industrial Technology Institute is a comprehensive well equipped Biotechnology laboratory possessing Molecular Biology, Microbiology, Plant tissue culture and Industrial enzyme fermentation platforms for the interdisciplinary research applications and services of biotechnology. (iti.lk)
  • The laboratory has equipments such as a Biosafety Level 2 (BSL 2) cabinet, incubator shaker and electroporator to support upstream experiments such as bacterial isolation and DNA extraction. (ukm.my)
  • online Marrying signalling shows when mutants show profitable as a transsatellite or a PhIP does demanding assessed back via the clone to new teachers of experiments around the laboratory. (bcinbergen.com)
  • We provide laboratory equipments, molecular tools, and consumables. (biosciregister.com)
  • A successful candidate will have a background in molecular biology, genetics, biochemistry, or related fields, hands-on experience with molecular biology techniques and laboratory automation, and a desire to grow and learn at Capsida. (astrixinc.com)
  • We're specialized in distributing & marketing of life science products, molecular services, laboratory consumables, equipment and instruments. (bio3.my)
  • Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments. (cdc.gov)
  • It is ranging from cloning and molecular development to production and characterization. (lu.se)
  • Phenol-chloroform extraction is a liquid-liquid extraction technique in molecular biology used to separate nucleic acids from proteins and lipids. (wikipedia.org)
  • The frozen sample is later thawed at 4° C and homogenized in an extraction buffer that dissolves all the cell's membranes to release nucleic acids (DNA and RNA). (noaa.gov)
  • An organic extraction is performed to remove proteins, fats, and other organically soluble compounds, leaving nucleic acids in the aqueous solution. (noaa.gov)
  • Molecular techniques make this possible through the isolation of important genes and the production of biomedical compounds through recombinant technology. (noaa.gov)
  • Sometimes particular E. coli strains are sub-optimal for plasmid extraction. (zymoresearch.com)
  • Molecular biology provides exciting new tools for discoveries that will advance biology, biochemistry, and medicine. (noaa.gov)
  • To identify the photoreceptive molecule that regulates photoperiodic responses, in the present study, we have cloned and characterized the cDNA encoding an opsin gene expressed in the ayu brain, a putative site of the photoreceptor for photoperiodism. (bioone.org)
  • Cloning of cDNA encoding a decapeptide pheromone (sodefrin) that attracts conspecific female newts was attempted. (wiley.com)
  • A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. (wiley.com)
  • Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. (wiley.com)
  • This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. (wiley.com)
  • HM038439), was 731 nucleotides long and encoded a partial protein of 124 amino acids with predicted molecular weight of 13.65 kDa and calculated 10.01 isoelectric point. (scialert.net)
  • Her Ph.D. work focused on understanding the molecular and genetic mechanisms underlying maize kernel composition traits (protein, oil and starch). (quarles.com)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)
  • Methods of DNA extraction. (classicbiotech.com)
  • In this article, we will discuss about the fascinating methods of DNA extraction. (classicbiotech.com)
  • DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components. (classicbiotech.com)
  • There are different physical and chemical methods of DNA extraction. (classicbiotech.com)
  • This article provides a comprehensive overview of the most commonly used chemical methods of DNA extraction, highlighting their principles, advantages, and limitations. (classicbiotech.com)
  • Chemical methods of DNA extraction play a crucial role in various fields of research and diagnostics. (classicbiotech.com)
  • This manual will `demystify' the methods in molecular microbial ecology for readers who are novice in the field but are excited by the prospects of the technology. (nhbs.com)
  • We recommend removing the fluorescent staining dye with the gel extraction kits or PCR clean up kits because these methods are convenient and high efficiency. (smobio.com)
  • Molecular Therapy - Methods and Clinical Development. (lu.se)
  • Methods in molecular biology (Clifton, N.J.). 1937 , p. 59-87 29 p. (lu.se)
  • In depth understanding and demonstrated competency in complex DNA cloning using restriction digestion and ligation, Gibson assembly, overlapping PCR assembly, Gateway cloning etc. (fems-microbiology.org)
  • After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
  • 5) Molecular Systematics, 2nd ed. (uconn.edu)
  • The course will focus on the basics of molecular systematics theory and practice from the point of view of the data. (uconn.edu)
  • Labs will cover basic techniques in molecular systematics from DNA extraction to sequencing, alignment and cloning. (uconn.edu)
  • 2) The week prior to the start of classes you will be given a checklist discussing practical considerations, organization and data checks for molecular systematics. (uconn.edu)
  • These Powerpoint presentations will be posted on the class website so that in the future when you teach a molecular systematics class, they can be used as a starting point to revise and develop lectures of your own. (uconn.edu)
  • Lecture 2.ASRV, models of evolution, and the history of molecular systematics. (uconn.edu)
  • The IUF - Leibniz Research Institute for Environmental Medicine investigates the molecular mechanisms through which particles, radiation and environmental chemicals harm human health. (fems-microbiology.org)
  • The McDonough lab's basic and translational approaches have been driven by challenging our understanding of the molecular mechanisms responsible for the maintenance of electrolyte homeostasis and control of blood pressure in the human body. (usc.edu)
  • Initially, her lab focused on regulation of the biosynthesis and assembly of sodium pump subunits and molecular mechanisms controlling sodium pump isoforms in kidney, cardiac and skeletal muscle by potassium, hormones, and other factors under normal conditions and during human heart failure. (usc.edu)
  • Tapping panel dryness (TPD) has become the mostimportant limiting factor for increasing natural rubber yield, whereas illuminating the molecular mechanisms underlying TPD is the prerequisite for solving the problem of TPD. (bvsalud.org)
  • However, molecular mechanisms underlying TPD are largely unknown. (bvsalud.org)
  • To reveal the molecular mechanisms underlying TPD, we sequenced and compared bark transcriptomes among healthy rubber tree, and TPD-affected ones at initial and advanced stages. (bvsalud.org)
  • Our results not only provide new insights into molecular mechanisms underlying TPD occurrence and development but also contribute to developing effective measures to control TPD in rubber trees. (bvsalud.org)
  • The molecular events and evolutionary forces underlying lethal mutagenesis of virus (or virus extinction through an excess of mutations) are not well understood. (biomedcentral.com)
  • The present study aimed to evaluate the serological and molecular prevalence of T. gondii among apparently healthy rural populations in four regions of Gabon. (bvsalud.org)
  • Specified Elective Courses" must exclusively be selected from amongst a list of courses within the categories of either "Pollution Control and Modeling", or "Molecular Ecology and Microbial Biotechnology", or "Socio-Ecological Sustainability" concentration areas. (boun.edu.tr)
  • DNA extraction is a fundamental step in molecular biology research, diagnostics, and forensic analysis. (classicbiotech.com)
  • The main PCR applications are inside fields such as diagnostics, cloning, sequencing and forensic analysis. (isogen-lifescience.com)
  • We also provide training for students, researchers and collaborators in the techniques of medium (agar/broth) preparation for bacterial cultures, antibiotic susceptibility testing (minimum inhibitory concentration determination), molecular cloning and population analysis for hetero-VISA detection. (ukm.my)
  • Axon Lab AG specializes in the field of diagnostics and molecular biology. (biosciregister.com)
  • Our primary area coverage includes molecular biology, cell culture, food and environmental quality testing, diagnostics, natural product testing , services etc. (bio3.my)
  • Extraction and analysis of GO annotations proved an extremely effective method for identifying generic processes associated with biochemical pathways, proving more efficient than solely analysing Blast data output. (nih.gov)
  • We seek a highly motivated candidate (f/m) with a solid background in genetics and molecular biology. (fems-microbiology.org)
  • Prevalence of Major Methicillin-Resistant Staphylococcus aureus Clones in Korea Between 2001 and 2008. (who.int)
  • The Platform possesses state-of-the-art equipments and expertise to carry out molecular biology based research. (iti.lk)
  • The staining dye can be removed from DNA with traditional ethanol precipitation, PCR clean up kits, or the gel extraction kits. (smobio.com)
  • The ethanol precipitation method can follow conventional molecular cloning or follow the listed protocol. (smobio.com)
  • Identification of these clones in specific libraries associated with desiccation validates the computational analysis by library rather than producing a global overview of all libraries combined. (nih.gov)
  • Proven experience in classical molecular analysis (e.g. (greencolab.com)
  • Molecular analysis identified five new phylogenetic SIV lineages. (cdc.gov)
  • With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. (bvsalud.org)
  • We will explore the ways in which an understanding of processes of evolution of molecular data can help in the construction of evolutionary trees. (uconn.edu)
  • The first is the antibiotic selection required for any molecular cloning experiment. (zymoresearch.com)
  • However, reports on the molecular prevalence of T. gondii in humans are rare in Gabon. (bvsalud.org)
  • Five normalised libraries were produced from both desiccating and rehydrating populations of O. arcticus from stages that had previously been defined as potentially informative for molecular analyses. (nih.gov)
  • les analyses génétiques réalisées sur six d'entre eux ont révélé une délétion de 12 bp (155-166 del) dans l'exon 3 localisé en 1q42-43 dans le gène TBCE codant la protéine chaperon E spécifique de la tubuline. (who.int)
  • Currently, with the application of molecular biology resources in human identification, it became possible to identify a person even without physical ante-mortem data or with deteriorated biological material in negligible amounts, which are actually relatively common conditions in forensic analyses 7,12,16,19 . (bvsalud.org)
  • The CTAB (cetyltrimethylammonium bromide) method commonly used for DNA extraction from plant tissues. (classicbiotech.com)
  • This book presents a comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants. (nhbs.com)
  • Thus, these modified E. coli strains are used as workhorses for molecular cloning and plasmid production. (zymoresearch.com)
  • Molecular tools have enhanced our strains [9]. (who.int)
  • Ensure the development of next generation molecular biology activities foreseen in the R&I agenda of GreenCoLab. (greencolab.com)
  • Molecular Depot LLC specialize in the development of research tools and biochemicals. (biosciregister.com)
  • The CC30 genomes were related to the South-West Pacific clone but formed a distinct, diverse lineage, with evidence of global dissemination. (who.int)
  • Purified DNA is immediately ready for routine molecular biology applications. (thomassci.com)
  • We, CELLFREE, offers cloning services so you can free yourself from routine gene cloning and instead devote your energy and time to more creative research. (cellfreeprotein.com)
  • Currently a Professor of Physiology and Neurosciences, Keck School of Medicine of USC in Los Angeles, CA, she earned degrees in Physiology at UC Berkeley (AB) and at the University of Hawaii (PhD), and was trained in molecular Physiology at UC San Francisco Cardiovascular Research Institute and Columbia University. (usc.edu)
  • Gene Bio Application Ltd. develops novel tools for research in molecular biology and medicine. (biosciregister.com)
  • Expertise in standard molecular biology techniques and cell culture is critical, and experience with plasmid cloning, gene editing and AAV vector production. (fems-microbiology.org)
  • The aim of this project was to generate clones and sequence data in the form of ESTs to provide a platform for the future molecular characterisation of the processes involved in protective dehydration. (nih.gov)
  • This lab will be of interest to both experienced and novice molecular systematists because we will try newly developed kits/techniques and compare them to older ones and we will pursue a class project. (uconn.edu)
  • Molecular biology techniques are important new tools for exploration and excavation of the ocean. (noaa.gov)
  • After resuspending the stored samples, a process called electrophoreses is used to visualize the DNA extractions and determine their concentration. (noaa.gov)
  • As a model system we used a polyclonal antibody to 3-phenoxybenzoic acid (3-PBA) and an anti-immunocomplex phage clone bearing the cyclic peptide CFNGKDWLYC. (cdc.gov)