Extracellular matrix cell proliferation. Medical search. Frequent questions

2011). Another EGFR ligand, TGF-, has been shown to enhance corneal epithelial migration and proliferation similar to Fenoterol EGF and HB-EGF, MRK whereas it inhibited the expression of the differentiation-related corneal epithelial marker keratin 3 (Wilson et Fenoterol al. (cancerrealitycheck.com)
The differentiation of endometrial stromal cells into decidual cells, termed decidualization, is an integral step in the establishment of pregnancy. (bioone.org)
Gene ontology and upstream regulator pathway analysis showed that WNK1 regulates cell migration, differentiation, and proliferation. (bioone.org)
LPA acts as an autocrine/paracrine messenger through at least six G protein-coupled receptors (GPCRs), known as LPA 1-6 , to induce various cellular processes including wound healing, differentiation, proliferation, migration, and survival. (hindawi.com)
In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. (frontiersin.org)
There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair. (frontiersin.org)
The impairment of proliferation, differentiation, and mineralization in osteoblast is the main pathophysiological mechanism of osteoporosis, which has been proven by previous studies ( 4 , 5 ). (frontiersin.org)
The aim of this study was to investigate the effects of Ishige sinicola , a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. (degruyter.com)
A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. (degruyter.com)
Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
In addition, we examined whether these substrates will support differentiation of retinal pigmented epithelial cells, an important, clinically relevant cell type that is currently in clinical trials for eye disease. (ca.gov)
This surface proved to be efficient at supporting growth of undifferentiated hESC as well as differentiation of useful cell types. (ca.gov)
There is a critical need for new technologies to facilitate growth and differentiation of human embryonic stem cells (hESC) using clinically acceptable, animal-free reagents. (ca.gov)
We propose to develop novel, well-defined, synthetic extracellular matrices for growth and differentiation of hESC. (ca.gov)
Our approach is to first understand how hESC interact with extracellular matrix materials by analyzing candidate adhesive substrate proteins and integrin receptors that mediate attachment, survival, proliferation and differentiation. (ca.gov)
Proven capable of recreating aspects of organ structure and function in vivo, organoids are organ-like structures that can be prepared by 3D cell culture and differentiation of stem cells or organ progenitors. (onenucleus.com)
Here, we report that adipocytes from tumor-invasive mammary fat undergo de-differentiation to fibroblast-like precursor cells during tumor progression and integrate into the tumor microenvironment. (usda.gov)
A series of cellular pathophysiological mechanisms involved in AS (e.g., differentiation, proliferation, and signalling pathways) are under the control of miRNAs. (hindawi.com)
I will present some of the work done on the detection of metabolites released by cells undergoing osteogenic differentiation and on the development of 3D architectures that can be used to host and monitor stem cells. (gla.ac.uk)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
CTGF is a cysteine-rich secretory protein and belongs to a six member family who are known to be involved in fundamental biological processes such as cell proliferation, attachment, migration, differentiation, wound healing and angiogenesis as well as in the development of several pathologic conditions including fibrosis and tumorigenesis. (cdc.gov)
High glucose increased TLR expression, myeloid differentiation factor 88, interleukin-1 receptor-associated kinase-1, and nuclear factor-κB (NF-κB) p65-dependent activation in THP-1 cells. (diabetesjournals.org)

The DEmRNAs were mainly enriched in tumorigenesis‑associated GO terms (angiogenesis, positive regulation of cell proliferation, positive regulation of fibroblast apoptotic processes and regulation of neutrophil migration) and pathways (Hippo signaling pathway, cancer pathways, and Wnt signaling pathway). (spandidos-publications.com)
High expression of lncRNA H19 promotes cell migration and enhances angiogenesis ( 11 , 12 ). (spandidos-publications.com)
5 The damaged brain tissue could be restored by angiogenesis, which provides oxygen and nutrients to improve recovery in the penumbra area, 6 and allows macrophages to clear necrotic nerve cells or tissues. (cbinsights.com)
The study of genes that regulate the processes involved somehow with endometriosis as ID2 (cell proliferation), PRELP (extracellular matrix) and SMOC2 (angiogenesis) may help clarify its development. (usp.br)

The ability of lovastatin, mevastatin and simvastatin to inhibit the melanoma cell proliferation was examined using cytotoxicity and apoptosis assays. (biomedcentral.com)
Organoids are proving increasingly popular because they maintain genetic information, are CRISPR compatible, expand tractable cell line availability, make sharing libraries easier and are proving useful in drug sensitivity assays. (onenucleus.com)
Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)
Although biochemical and biophysical assays and cell biology experiments advance these insights, full elucidation of the pathophysiology caused by human disease requires a broader approach, including assessment of the impact that cells with a pathogenic mutation have on neighboring cells that do not express the mutant protein and consideration of system-wide responses to a diseased organ. (rupress.org)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
The current investigation aimed to demonstrate how interpretation of results from several standard cytotoxicity and proliferation assays vary in dependence on contributions from the pentose phosphate pathway (PPP). (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

In addition, at 48H, BPs linked to inflammatory phenotypes were activated (confirming KEGG results), while BPs linked to extracellular matrix (ECM) structural organisation, endothelial cell proliferation, and collagen metabolism were repressed. (nature.com)

For example, lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is highly expressed in GBM tissues and perturbed expression of NEAT1 suppresses cell proliferation and invasion ( 10 ). (spandidos-publications.com)
When lncHERG is highly expressed, cell multiplication, invasion and migration are inhibited in GBM ( 16 ). (spandidos-publications.com)
We examined the effects of statin drugs on in vitro proliferation, migration and invasion of melanoma cells. (biomedcentral.com)
Effects on cell migration and invasion were assessed using transwell invasion and migration chambers. (biomedcentral.com)
Lovastatin, mevastatin and simvastatin inhibited the growth, cell migration and invasion of HT144, M14 and SK-MEL-28 melanoma cells. (biomedcentral.com)
However, higher doses of statins may have a role to play in adjuvant therapy by inhibiting growth and invasion of melanoma cells. (biomedcentral.com)
The extracellular matrix (ECM) interacts with tumor and stromal cells to promote cancer cells proliferation, migration, invasion, a. (biomedcentral.com)
AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion, and proliferation. (onenucleus.com)
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)
This study aims to understand the function and mechanism of Drosophila Glycine N-acyltransferase (GLYAT) in cell invasion. (sdbonline.org)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
Matrix metalloproteases (MMPs) are zinc-dependent proteinases that participate in extracellular matrix degradation 1-3 and favor the invasion and proliferation of tumor cells 4 . (bvsalud.org)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase that regulates cell signaling, proliferation, migration, and development. (rcsb.org)
Upon cell adhesion to the extracellular matrix, FAK is being translocated toward focal adhesion sites and activated. (rcsb.org)
Adult SVZ progenitor cells express the laminin receptor alpha6beta1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche. (nih.gov)
First, we characterized the cell surface receptors for matrix proteins on stem cells and identified specific proteins that will support adhesion and growth of the cells. (ca.gov)
Second, using an interdisciplinary approach, we developed a novel method to screen peptides from these proteins for their ability to support adhesion and proliferation of the stem cells. (ca.gov)
Based on our characterization of cell surface receptors for matrix proteins on stem cells completed in the first year, we went on to test specific, purified proteins that will support adhesion and growth of the cells. (ca.gov)
Second, using an interdisciplinary approach, we developed novel hydrogels using click chemistry that support adhesion and proliferation of the stem cells. (ca.gov)
It also interacts directly or indirectly with cells to regulate such physiological processes as cell adhesion, survival, proliferation, and motility. (rndsystems.com)
Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
Interference RNA-mediated knockdown of GPR56 recapitulates the loss of adhesion seen in knock-outs, and reexpression of GPR56 rescues the adhesion defect in knock-out granule cells. (jneurosci.org)
cancer cell growth and spreading, it has also been proposed that GPR56 regulates cell adhesion, but this has remained untested in the nervous system. (jneurosci.org)
Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
These results support a novel hypothesis that the cerebellar defects in Gpr56 knock-outs, and potentially in human patients with GPR56 mutations, arise from the disrupted adhesion of developing granule cells to the pial BM. (jneurosci.org)

Thus, we hypothesized that pericytes regulate β-cells through the production of BM components. (nature.com)
We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
PGs bind growth factors, regulate proteolytic activity and act as matrix receptors. (lu.se)

The miR-128-5p mimic promoted the migration and proliferation of VSMCs and suppressed p21, p27, and SM22ɑ expression. (hindawi.com)
Several studies have shown that miRNAs play multiple roles in the phenotypic transformation, migration, and proliferation of VSMCs by inhibiting ERK1/2 activation [ 11 , 12 ], partly by regulating the tissue inhibitor of metalloproteinases (TIMPs)-MMPs and p21-cyclins interactions [ 13 - 18 ]. (hindawi.com)

It plays important structural roles, forming loose, hydrated matrices during development and disease. (rndsystems.com)

Single-cell sequencing reveals that these de-differentiated adipocytes lose their original identities and transform into multiple cell types, including myofibroblast- and macrophage-like cells, with their characteristic features involved in immune response, inflammation, and extracellular matrix remodeling. (usda.gov)
It has been shown that inflammation caused by oxidized low-density lipoprotein (ox-LDL) contributes to the occurrence and development of AS [ 1 ], which can promote vascular smooth muscle cell (VSMC) migration, proliferation, and transformation from a contractile to a synthetic phenotype [ 2 - 6 ]. (hindawi.com)
CONCLUSIONS: The altered expression patterns of glypican and syndecan-1 and -4 in chronic ulcers reflect their possible roles during inflammation and cell proliferation. (lu.se)
Processes involved in atherosclerosis include coagulation, inflammation, lipid metabolism, intimal injury, and smooth muscle cell proliferation (see the image below). (medscape.com)
Chronic wounds fail to proceed through the normal phases of wound healing (ie, hemostasis, inflammation, proliferation, and remodeling), typically stalling in the inflammatory phase. (medscape.com)

Primary human endometrial stromal cells (HESCs) were subjected to small interfering RNA knockdown of WNK1 followed by in vitro decidualization. (bioone.org)
Through in vivo and in vitro experiments, we found that it promotes the proliferation, migration, and tubular formation of brain microvascular endothelial cells (BMECs). (cbinsights.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
In vitro maintenance of multipotent neural crest stem cells as crestospheres. (lu.se)
To complement the in vivo system of chick embryos, we also employ human cell cultures, chick neural crest derived in vitro crestospheres and in vivo mice models. (lu.se)

It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
At the age of defect onset, GPR56 expression is restricted specifically to developing granule cells in the rostral cerebellum, suggesting that GPR56 regulates properties of these cells. (jneurosci.org)

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and cell proliferation assay were used to explore the functional role of the key lncRNAs in GBM. (spandidos-publications.com)
Today, AMSBIO offers researchers an unmatched range of high-performance stem-cell-optimized and qualified growth media, sera, feeder cells, supplements, growth factors and assay kits. (onenucleus.com)
The cell survival rate was measured using the principle of water-soluble tetrazolium salt-1 assay, which is a measurement method for cell survival rate. (researchgate.net)
Senescence-associated β-galactosidase (SA-β-gal) assay was implemented to dye β-galactosidases (used as a cell aging biomarker) to measure HDF cell aging by UVA. (researchgate.net)

Transmembrane protein 88 inhibits transforming growth factor-β1-induced-extracellular matrix accumulation and epithelial-mesenchymal transition program in human pleural mesothelial cells through modulating TGF-β1/Smad pathway. (nih.gov)
TMEM88 inhibits extracellular matrix expression in keloid fibroblasts. (nih.gov)
HIV-1 gp120 inhibits TLR9-mediated activation and IFN-α secretion in plasmacytoid dendritic cells. (bdbiosciences.com)

Thus, it may refer to the "exosome complex ," being a proteic (enzymatic) macromolecular machinery, present in archaea and eukaryotic cells, being involved in RNA degradation. (frontiersin.org)
Chronic wounds typically do not progress through the normal phases of wound healing and generally remain stagnant during the inflammatory phase, resulting in an increase in proteolytic enzymes with degradation of the extracellular matrix. (medscape.com)

In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
We proposed to develop novel, well-defined, synthetic extracellular matrices that support survival and proliferation of human embryonic stem cells. (ca.gov)

Create versatile scaffold solutions for tissue repair, cell proliferation, hemostasis and carriers for your APIs. (dsm.com)
In order to control biofilm formation, sequester proteolytic enzymes, and provide a biocompatible scaffold to support healing, the investigators utilize a purified collagen matrix containing polyhexamethylene biguanide (PCMP) in a case series of 9 wounds on 8 patients with multiple comorbidities who did not respond to previous conventional or adjuvant therapy. (medscape.com)

Mediators and course of events during stromal healing have been detailed, and new treatment regimens including gene (decorin) and stem cell therapy for excessive healing have been designed. (cancerrealitycheck.com)
Attention is also paid to problems in wound healing understanding and treatment, such as lack of specific epithelial stem cell markers, reliable identification of stem cells, efficient prevention of haze and stromal scar formation, lack of data on wound regulating microRNAs in keratocytes and endothelial cells, as well as virtual lack of targeted systems for drug and gene delivery to select corneal cells. (cancerrealitycheck.com)
The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)

Alizarin red S staining demonstrated that ISE treatment tended to increase extracellular matrix calcium accumulation. (degruyter.com)
It was found that GSOs reversed GLU-induced reductions in cell viability and the LDH release rate, and rescued ROS accumulation. (sdbonline.org)

Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
AA and the extracellular regulated protein kinase 1/2 (ERK1/2) blocker U0126 markedly inhibited migration, elevated smooth muscle 22 α (SM22 α ) expression, repressed VSMC proliferation, elevated miR-466f-3p and miR-425-3p expression, and suppressed miR-27a-5p and miR-128-5p expression in ox-LDL-induced VSMCs. (hindawi.com)

Cell death escape is one of the most prominent features of tumor cells and closely linked to the dysregulation of members of the Bcl-2 family of proteins. (biomedcentral.com)
The de-differentiated cells are metabolically distinct from tumor-associated fibroblasts but exhibit comparable effects on tumor cell proliferation. (usda.gov)
Collectively, the metabolic interplay between tumor cells and adipocytes induces adipocyte mesenchymal transition and contributes to reconfigure the stroma into a more tumor-friendly microenvironment. (usda.gov)
We focus on the childhood tumor form neuroblastoma, originating from cells of the sympathetic nervous system (SNS) in turn derived from the transient neural crest stem cell population. (lu.se)

These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell. (wikipedia.org)
Once these molecules are processed, they arrange themselves into long, thin fibrils that cross-link to one another in the spaces around cells. (wikipedia.org)
OThe molecules of the extracellular matrix play an important role during cell development and morphogenesis. (bvsalud.org)

The altered protein interacts abnormally with other collagen-building proteins, disrupting the structure of type I collagen fibrils and trapping collagen in the cell. (wikipedia.org)
Because only one copy of the gene is directing the cell to make pro-alpha1(I) chains, cells from people with this disorder make only half of the normal amount of type I collagen, which results in bone fragility and other symptoms. (wikipedia.org)
DSM portfolio of innovative biomedical materials and process manufacturing technologies: Biomedical Polyethylenes, Biomedical polyurethanes, Collagen Technology, Device Development, and Extracellular Matrix (ECM) Technology. (dsm.com)

Hence, recapitulating the islet ECM in both isolated islets and stem-cell-derived β-cells is of high therapeutic value. (nature.com)
There is an emerging understanding of the importance of the vascular system within stem cell niches. (nih.gov)
Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. (nih.gov)
This is an important, unmet need in the field and development of such a substrate would aid in moving stem cell therapies to the clinic. (ca.gov)
AMSBIO , a provider of cutting-edge products and services for accelerating stem cell research, has sponsored two aspiring life scientists to attend and present posters at the recent ISSCR 2023 conference in Boston, USA. (onenucleus.com)
Fellow travel grand award winner Kerstin Filippi from Dr Michael Hesse's group was impressed by "How many important people in the field of stem cell research were there. (onenucleus.com)
For over 20 years - AMSBIO has aimed to help advance stem cell discovery by supplying research scientists with the necessary tools and reagents needed to work more efficiently and obtain reliable results faster. (onenucleus.com)
The company provides unique clinical grade products for stem cell and cell therapy applications. (onenucleus.com)
Hematopoietic Stem Cell Transplantation Hematopoietic stem cell (HSC) transplantation is a rapidly evolving technique that offers a potential cure for hematologic cancers ( leukemias, lymphomas, myeloma) and other hematologic disorders. (msdmanuals.com)
Living donors are often used for kidney and hematopoietic stem cell transplants, less frequently for segmental liver transplants, and rarely for pancreas and lung transplants. (msdmanuals.com)
Enhanced expression of MycN/CIP2A drives neural crest toward a neural stem cell-like fate: Implications for priming of neuroblastoma. (lu.se)
RESULTS: We demonstrated that chemotherapy-stimulated CAFs promoted tumorigenic and stem cell-like properties of NSCLC cells, which contribute to their chemoresistance. (cdc.gov)
Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)
CONCLUSION: Our results show that PDL-1-positive CAFs modulate stem cell-like properties of NSCLC cells by secreting elevated HGF, thereby promoting chemoresistance. (cdc.gov)

1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Phenotypic and functional characterization of bone marrow mesenchymal stem cells derived from patients with multiple myeloma. (bdbiosciences.com)
However, potentially erroneous characterization of cytotoxicity and cell proliferation can arise if verification of baseline interaction of test article with method employed is neglected. (cdc.gov)
These results demonstrate differential sensitivity of standard cytotoxicity assessments on the PPP, thus (1) decoupling 'mitochondrial activity' as an interpretation of cellular formazan and Alamar Blue metabolism, and (2) demonstrating the implicit requirement for investigators to sufficiently verify interaction of these methods in routine cytotoxicity and proliferation characterization. (cdc.gov)

But, when we change the metabolism of these cells, we can slow down the growth of the breast cancer cells in our animal study. (usda.gov)

The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)
We identified 715 upregulated DEGs and 603 downregulated DEGs between MC3T3-E1 cells with and without osteoblast induction by analyzing the raw data of the GSE46400 dataset. (frontiersin.org)
The RT-qPCR also determined declined expressions of miR-204-5p and miR-24-3p concomitant with elevated expressions of miR-211-5p, miR-3470b, miR-466b-3p, and miR-466c-3p in the MC3T3-E1 cells, with osteoblast induction compared with undifferentiated MC3T3-E1 cells. (frontiersin.org)
MiR-211-5p upregulation by its mimic increased Arhgap11a expression in MC3T3-E1 cells. (frontiersin.org)

13 Thus, the application of radiation therapy following surgery appears to prevent keloid scar recurrence by decreasing fibroblast proliferation, arresting the cell cycle and inducing premature cellular senescence. (jcadonline.com)

2011). EGFRs comprise a family of four receptors, of which EGFR1 is the most studied in many cells and tissues including the cornea. (cancerrealitycheck.com)

The viability of GBM cells was significantly decreased following RNA interference with DLEU1 and TRAF4 production. (spandidos-publications.com)
mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
STEM CELLBANKER significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage. (onenucleus.com)
It is one of the chief components of the extracellular matrix, contributes significantly to cell proliferation and migration. (vitanatural.net)

VSMCs were transfected with the miR-128-5p mimic and inhibitor, and the migration, phenotypic modulation, and proliferation of VSMCs were investigated. (hindawi.com)

The results demonstrate that DLEU1 and TRAF4 is highly expressed in GBM tissues and promotes proliferation of GBM cells. (spandidos-publications.com)
Versican is a large extracellular matrix proteoglycan that is present in a variety of tissues. (rndsystems.com)

Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. (nih.gov)
Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)

11 Department of Molecular and Cell Biology, Verna and Marrs McLean Department of Biochemistry, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, Texas. (aacrjournals.org)
This study aims to clarify the time-dependent molecular pathways by which TMAO mediates endothelial dysfunction through transcriptomics and metabolomics analyses in human microvascular endothelial cells (HMEC-1). (nature.com)
Deciphering the molecular and cellular atlas of immune cells in septic patients with different bacterial infections. (nih.gov)
The journey from defining genetic etiology to molecular understanding of disease mechanism can be frustratingly slow, requiring exploration of how mutations alter functions of the encoded protein and delineation of the responses that they trigger in relevant cells. (rupress.org)

The concentrations required to inhibit proliferation of melanoma cells (0.8-2.1 μM) have previously been achieved in a phase I clinical trial of lovastatin in patients with solid tumours, (45 mg/kg/day resulted in peak plasma concentrations of approximately 3.9 μM). (biomedcentral.com)
Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)

Finally, we identified a cyclic RGD peptide that supports growth of human embryonic stem cells. (ca.gov)
Stem cells, particularly human embryonic stem cells, are a notoriously labile cell type. (onenucleus.com)

7 Besides, new vascular endothelial cells can release a variety of cytokines to nourish nerve cells and induce neurogenesis, and communication between nerves could also be repaired. (cbinsights.com)
A large number of cytokines, extracellular matrix (ECM), and matrix metalloproteinases (MMPs) are synthesized and secreted by synthetic VSMCs during the progression of AS [ 7 , 8 ]. (hindawi.com)
[ 1 , 4 , 5 ] The chronic wound environment is characterized by excessive and persistent levels of proinflammatory cytokines, leading to elevated levels of proteases that degrade the extracellular matrix and prolong the inflammatory phase. (medscape.com)

Genes involved in cell proliferation and extracellular matrix production are down-regulated while genes promoting apoptosis are upregulated. (jcadonline.com)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

It is concluded that dermal application of a contact allergen, as exemplified by OX and HCA, may induce cell proliferation in the neighboring lymph nodes and spleen indicative of hapten and/or haptenated proteins diffusing through the skin to periph- eral nodes and the blood to produce systemic sensitization. (cdc.gov)

Here, we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using three-dimensional whole mounts, confocal microscopy, and automated computer-based image quantification. (nih.gov)

Whereas α2 laminins promoted islet cell clustering, they did not affect gene expression. (nature.com)

Furthermore, downregulation of phosphorylated and/or Fenoterol total EGFR by miR-146a or miR-424 led to a delayed wound healing in cultured telomerase-immortalized human corneal epithelial cells (Funari et al. (cancerrealitycheck.com)
2013) and in primary limbal epithelial cells (Winkler et al. (cancerrealitycheck.com)
2010). Several studies also suggest that it stimulates better wound healing responses than EGF in corneal epithelial cells (Xu et al. (cancerrealitycheck.com)

The EV composition depends on the producer cell type and its physiological conditions. (frontiersin.org)

Our experiments demonstrate that GPR56 is essential for proper morphogenesis of the rostral cerebellum and suggest that defects caused by loss of GPR56 arise from granule cell abnormalities in this region. (jneurosci.org)

CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Within vascular smooth muscle cells, smooth muscle α-2 actin forms the core of structures called sarcomeres, which are necessary for muscles to contract. (medlineplus.gov)
This study aimed to identify the role of AA in oxidized low-density lipoprotein (ox-LDL)-induced vascular smooth muscle cell (VSMC) behaviours and to explore the effects of microRNAs (miRNAs). (hindawi.com)

Received in revised form 15 December 2008 nodes for assessment of lymphocyte proliferation as a marker of sensitization. (cdc.gov)

Exp Cell Res, 2020 Oct 15. (nih.gov)

shRNA-mediated suppression of and genes by adenoviral gene therapy of whole corneal epithelium or only of limbal cells normalized epithelial wound healing altered in human diabetic organ-cultured corneas with restoration of signaling pathways mediated by EGFR-Akt axis (Saghizadeh et al. (cancerrealitycheck.com)

Osteoblast mainly originates from mesenchymal stem cells (MSCs), and it is involved in bone formation, remodeling, and reconstruction via the formation of a bone matrix and regulation of bone resorption ( 6 ). (frontiersin.org)

Cell viability and reactive oxygen species (ROS) generation were also evaluated. (nature.com)
TMAO treatment for either 24H or 48H induces reduced cell viability and enhanced oxidative stress. (nature.com)

The Concept of Cancer Stem Cells: Elaborating on ALDH1B1 as an Emerging Marker of Cancer Progression. (lu.se)

Natural killer (NK) cell is a specialized immune effector cell type that plays a critical role in immune activation against abnormal cells. (biomedcentral.com)
The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
Recipients of grafts containing immune cells (particularly bone marrow, intestine, and liver) are at risk of graft-vs-host disease, in which the donor immune cells attack recipient tissue. (msdmanuals.com)

The purpose of this study was to evaluate the effects of in vivo cyclical digit loading on CTGF- positive staining cell density in the Flexor Digitorum Profundus (FDP) tendon at the epicondyle. (cdc.gov)
In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

We explored whether LPL affects (a) lipoprotein transport across bovine aortic endothelial cells or (b) lipoprotein binding to subendothelial cell matrix (retention). (jci.org)

matrix metalloproteases, extracellular matrix, odontogenic lesions. (bvsalud.org)

These conflicting reports highlight the need for further studies on the effects of statins on cancer cells. (biomedcentral.com)
These transformed cells possess different metabolic states, act differently, and help the cancer cells grow. (usda.gov)
Additional studies are needed to better the association between fat and cancer cells. (usda.gov)
In the USA, obesity has recent- obesity is associated with progres- aberrations of cancer cells, summa- ly surpassed tobacco use as the sion but not incidence [9]. (who.int)

Promising new technologies with potential for corneal wound Fenoterol healing manipulation including microRNA, induced pluripotent stem cells to generate corneal epithelium, and nanocarriers for corneal drug delivery are discussed. (cancerrealitycheck.com)
However, HB-EGFR remains bound to cell much longer than EGF and has a lasting effect on wound healing after brief treatment, consistent with immobilized pool of HB-EGF on the cell surface and ECM providing continuous EGFR stimulation (Tolino et al. (cancerrealitycheck.com)
Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)
Whereas the expression of syndecan-4 and glypican in intact skin was mostly in the pericellular regions of keratinocytes, the epidermal cells from the wound edge contained mostly intracellular PGs. (lu.se)
In the wound edge, syndecan-4 was predominantly expressed by epidermal basal layer cells. (lu.se)
[ 4 ] In contrast to planktonic, or free-floating, bacteria, microbes in the chronic wound bed are believed to exist mainly in biofilm communities, which attach to the wound surface and exist in microcolonies enclosed in a protective matrix of polysaccharide material. (medscape.com)

Anatomy43

Organisms11

Diseases10

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes48

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Differentiation28

Angiogenesis4

Assays7

Endothelial cell1

Invasion13

Adhesion13

Regulate3

Migration and proliferation2

Structural1

Inflammation5

Vitro6

Regulates2

Assay4

Inhibits3

Degradation2

Survival2

Scaffold2

Stromal5

Accumulation2

Protein kinase3

Tumor4

Molecules3

Collagen3

Stem cell14

Progenitor cells2

Characterization3

Metabolism1

MC3T3-E14

Fibroblast1

Receptors1

Significantly5

Mimic1

Tissues2

Microenvironment2

Molecular4

Inhibit2

Embryonic stem2

Cytokines3

Apoptosis2

Induce1

Neural stem1

Gene expression1

Epithelial cells3

Physiological1

Morphogenesis1

Markers2

Vascular smooth2

Lymphocyte1

20201

Pathways1

Osteoblast1

Viability2

Progression1

Immune3

Vivo2

Bovine aortic endothelial1

Lesions1

Cancer Cells4

Wound7