ProteinsDegrade extracellular matrixIntegrinsMetalloproteinasesCollagenEpithelial cellsProteinAdhesionTissueMoleculesLaminaBasalModulatesCellsInternal limitingTissuesCytoplasmicGlycoproteinsLipidIsletMetalloproteinaseRetinalInteractionsFibronectinVitroIntegrinInvasionAccumulationVascularPerlecanAlterationsTumorsFibroblastsMorphogenesisCornealFibersComponentsBacterialFoundDefectsInvolvementThinAssembly
Proteins12
- The trimeric proteins intersect, composing a cruciform structure that is able to bind to other molecules of the extracellular matrix and cell membrane. (wikipedia.org)
- The proteins also bind to cell membranes through integrins and other plasma membrane molecules, such as the dystroglycan glycoprotein complex and Lutheran blood group glycoprotein. (wikipedia.org)
- Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold. (mayo.edu)
- He is known for his work on laminins - huge proteins that are a key component of all basement membranes. (imperial.ac.uk)
- Zinc is also an antioxidant and can directly protect cell membrane lipids and proteins and mercaptan-dependent macromolecules (tubulin and enzyme) from oxidative damage [ 6 ]. (nature.com)
- Further study applying proteomic analysis confirmed that CAFs secrete more proteases, extra-cellular matrix proteins, and proteins that modify the basement membrane compared with NAFs, pointing to a remodeling role for CAFs in invasion. (medindia.net)
- This protein is found in the extracellular matrix, which is the intricate lattice of proteins and other molecules that forms in the spaces between cells. (medlineplus.gov)
- An Illustration of the Laminin-111 complex depicting the domain organization Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. (pearltrees.com)
- The brain extracellular matrix (ECM), consisting of proteins and glycosaminoglycans, is a critical scaffold in the development, homeostasis, and disorders of the central nervous system (CNS) and undergoes remodeling in response to environmental cues. (biorxiv.org)
- Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)
- however, in the corneal stroma, excessive infiltration of monocytes/macrophages is considered to be unfavorable because they secrete matrix metalloproteinases (MMPs) and other proteins undesirable for tissue healing. (medscape.com)
- COPD-derived AECs proliferated as healthy AECs with few differences in gene and protein expression while retaining increased expression of disease marker HLA-A. The AEC2 expressed basement membrane components and a complex set of interstitial ECM proteins. (lu.se)
Degrade extracellular matrix2
- The main function of metalloproteinases is to degrade extracellular matrix and basement membrane components. (medscape.com)
- The metalloproteinase-9 (MMP-9) or gelatinase B belongs to the family of matrix metalloproteinases, enzymes which degrade extracellular matrix (ECM) and basement membrane components and are implicated in the carcinogenesis process, contributing to tumor progression, invasion and metastasis. (bvsalud.org)
Integrins3
- Flow-induced NF-κB activation is downstream of conformational activation of integrins, resulting in new integrin binding to the subendothelial extracellular matrix and signaling. (rupress.org)
- Dr. Yamada's group explores how coordination between the three-dimensional (3D) extracellular matrix of connective tissue or basement membranes, integrins, signal transduction, and cytoskeleton mediates or regulates key biological processes - cell adhesion, migration, matrix remodeling, tissue morphogenesis, and cancer cell invasion. (nih.gov)
- The laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and other receptors. (embl.de)
Metalloproteinases6
- The results showed that formononetin downregulated the expression of the key pro-angiogenic factors, including vascular endothelial growth factor (VEGF) and matrix metalloproteinases. (spandidos-publications.com)
- Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
- For example, neutrophils are short-lived and package active serine proteinases in azurophil (primary) granules and matrix metalloproteinases (MMP-8, MMP-9) in specific (secondary/tertiary) granules. (atsjournals.org)
- VEGF paves the way for new blood vessel formation by upregulating matrix metalloproteinases production by endothelial cells in the limbal vascular plexus. (aao.org)
- Matrix metalloproteinases (MMPs) are a group of structurally related endopeptidases that require a metal cofactor. (medscape.com)
- Direct invasion by carcinoma cells of the stromal compartment involves active proteolysis effected principally by matrix metalloproteinases (MMPs), while degrading the BM and other ECM that lie in the path of invading tumor cells, MMP-expressing cells also liberate growth factors that are sequestered there, thereby fostering cancer cell proliferation. (medscape.com)
Collagen7
- Fibroblasts are most commonly found in connective tissue that synthesizes extra-cellular matrix and another main structural protein of animals, collagen. (medindia.net)
- The researchers then devised an assay that deployed native basement membrane to separate cancer cells on one side and fibroblasts embedded in collagen on the other. (medindia.net)
- In asthma, the increased thickness of the basement membrane appears to be an important and localized feature of fibrosis because collagen deposition does not appear to be present deeper in the submucosa ( 2 ). (atsjournals.org)
- No specific or efficient treatment exists for Alport syndrome, an X-linked hereditary disease caused by mutations in collagen type IV, a crucial component of the glomerular basement membrane. (hindawi.com)
- The formation of AGEs results in collagen accumulation in the periodontal capillary basement membranes, causing membrane thickening. (health.am)
- [9] The use of steroids (such as cortisone) in conjunction with heparin and cyclodextrins causes a greater antiangiogenic effect, leading to the development of 'angiostatic steroids', which are thought to modulate collagen metabolism that can completely disintegrate the basement membrane of the blood vessels. (aao.org)
- A CMD Subtype that results from gene defects affecting the production of collagen type 6 protein in the extracellular matrix of the muscle. (cmdir.org)
Epithelial cells3
- The enzyme metalloproteinase degrades the cornea's basement membrane and extracellular matrix, while proteolytic enzymes allow vascular epithelial cells to enter the stromal layer of the cornea. (aao.org)
- explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
- It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. (bvsalud.org)
Protein7
- They are major constituents of the basement membrane, namely the basal lamina (the protein network foundation for most cells and organs). (wikipedia.org)
- Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). (scielo.br)
- TGF-β2 is capable of inducing the expression of ECM and basement membrane components in cultured ONH astrocytes via CTGF and upregulated TSP-1, a protein naturally involved in the activation of latent TGF-β. (arvojournals.org)
- They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)
- Laminin IV domain is also found in the perlecan protein, an integral component of basement membranes, which serves also as an attachment substrate for cells, but it is not found in short laminin chains (alpha4 or beta3). (embl.de)
- They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
- Vinculin, a plasma protein, links fibers to talin, which is a cell membrane protein. (medscape.com)
Adhesion5
- Abnormal laminin-332, which is essential for epithelial cell adhesion to the basement membrane, leads to a condition called junctional epidermolysis bullosa, characterized by generalized blisters, exuberant granulation tissue of skin and mucosa, and pitted teeth. (wikipedia.org)
- Indeed, granule cells from the rostral region of perinatal Gpr56 −/− cerebella show loss of adhesion to extracellular matrix molecules of the pial basement membrane. (jneurosci.org)
- Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
- Muscle contraction alters hemicentin dynamics at the B-LINK: a newly identified basement membrane adhesion system that connects tissues. (duke.edu)
- A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
Tissue13
- The long arm is capable of binding to cells and helps anchor organized tissue cells to the basement membrane. (wikipedia.org)
- Laminins are integral to the structural scaffolding of almost every tissue of an organism-secreted and incorporated into cell-associated extracellular matrices. (wikipedia.org)
- Current Challenges and Future Promise for Use of Extracellular Matrix Scaffold to Achieve the Whole Organ Tissue Engineering Moonshot. (mayo.edu)
- Basement Membrane of Tissue Engineered Extracellular Matrix Scaffolds Modulates Rapid Human Endothelial Cell Recellularization and Promote Quiescent Behavior After Monolayer Formation. (mayo.edu)
- Tissue engineered bovine saphenous vein extracellular matrix scaffolds produced via antigen removal achieve high in vivo patency rates. (mayo.edu)
- It offers a comprehensive resource of tissue-specific and sex-specific aging dysregulations and highlights age-related intercellular communication changes widespread across the whole body, such as the upregulation of immune system processes and inflammation, the downregulation of developmental processes, angiogenesis and extracellular matrix organization and the deregulation of lipid metabolism. (nature.com)
- Standing between a cancer cell in situ and the surrounding tissue of fibroblasts and extra-cellular matrix is the basement membrane, a thin sheet of fibers that normally cradles the cells above it. (medindia.net)
- 1 2 In tissue culture experiments in trabecular meshwork (TM) cells, TGF-β2 augmented the synthesis of extracellular matrix (ECM) and its cross-linking by transglutaminases. (arvojournals.org)
- GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
- Tissue remodeling due to extracellular matrix production and fibroblasts proliferation is directly modulated by growth factors classified according to their biological properties into pro-fibrotic and non-fibrotic growth factors ( 6 ). (atsjournals.org)
- Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. (embl.de)
- pan-ECM enables imaging of ECM compartments in live brain tissue, including the interstitial matrix, basement membrane (BM), and perineuronal nets (PNNs), and even the ECM in glioblastoma and stroke mouse brains. (biorxiv.org)
- It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
Molecules1
- The researchers are currently testing a role of CAF-derived molecules in basement membrane remodeling to further dissect the secret alliance between cancer cells and CAFs in basement membrane invasion. (medindia.net)
Lamina1
- The basal lamina (lamina - layers), also known as the basement membrane, is a specialised form of extracellular matrix . (cmdir.org)
Basal2
- Morphologic alterations of the extracellular matrix in diabetes include thickening of the basement membrane and, as evidenced in the corneal epithelium, a decrease in the area of the basal cell membrane occupied by hemidesmosomes. (nih.gov)
- Se localiza en la membrana basal de la lámina lúcida y funciona uniendo las células epiteliales a las membranas basales. (bvsalud.org)
Modulates1
- Shklover J, McMasters J, Alfonso-Garcia A, Higuita ML, Panitch A, Marcu L, Griffiths L . Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function. (mayo.edu)
Cells23
- β-Cells depend on the islet basement membrane (BM). (nature.com)
- The peri-islet membrane, which encapsulates the islets, separates the endocrine cells from the exocrine pancreas and serves as a barrier from immune cell infiltration to the islets. (nature.com)
- Thus, human endocrine cells are not in direct contact with the vascular BM components but with the invaginated peri-islet membrane. (nature.com)
- Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
- Furthermore, altering the extracellular matrix to promote p38 activation in cells on fibronectin suppresses NF-κB activation, suggesting a novel therapeutic strategy for treating atherosclerosis. (rupress.org)
- do cancer cells invade the basement membrane or do some fibroblasts help invading cancers? (medindia.net)
- Researchers at the Institut Curie in Paris now say that they have evidence of a coordinated attack on the basement membrane by cancer cells in situ and CAF cells in the extra-cellular matrix that begins long before the actual trans-location of cancer cells. (medindia.net)
- Using human colon cancer cells and primary human fibroblasts isolated from tumors and adjacent normal tissues, Alexandros Glentis and colleagues at the Institut Curie addressed the question of whether the cancer cells or the CAF cells were responsible for the breakdown of the basement membrane that leads to cancer progression. (medindia.net)
- Through imaging the tumors and fibroblasts, the researchers then found the smoking gun of cancer-CAF cooperation--long protrusions like puppet-strings that the tumor cells used to communicate with the fibroblasts well before the cancer cells moved to breach the basement membrane. (medindia.net)
- Specifically, it is found in part of the extracellular matrix called the basement membrane, which is a thin, sheet-like structure that separates and supports cells in many tissues. (medlineplus.gov)
- The major observation considered to be involved in the pathogenesis of retinal lesions is abnormalities in the internal limiting membrane formed by Müller cells, which is corresponding to the glia limitans formed by astrocytes in the brain. (intechopen.com)
- This finding suggests that asymmetrical distribution of phospholipids in the external membranes of eukaryotic cells may represent an important factor in determining the specificity of antibacterial peptides for targeting bacteria rather than eukaryotic cells. (upenn.edu)
- A significant correlation was found between the number of epithelial or submucosal cells expressing TGF- β in both asthma and chronic bronchitis and basement membrane thickness and fibroblast number. (atsjournals.org)
- Chronic bronchitis is another chronic obstructive disease of the airways in which inflammation is a continuous process probably commencing with an increase in inflammatory cells ( 4 ) and followed by airway remodeling with extracellular matrix deposition on the airways wall ( 5 ). (atsjournals.org)
- Dr. Yamada's research emphasizes the characterization of the dynamic movements of cells and their extracellular matrix as tissues are remodeled in 3D in real time. (nih.gov)
- Defining the cells and proteinases responsible for destruction of lung extracellular matrix associated with cigarette smoking will be required for development of appropriate proteinase inhibitors for application in COPD. (atsjournals.org)
- Isolation not only disrupts the internal vascularization and innervation of islets, but also fundamentally changes interactions between islet cells and macromolecules of the extracellular matrix (ECM). (northwestern.edu)
- Hyperglycemia results in increased gingival crevicular fluid glucose levels, which may significantly alter periodontal wound-healing events by changing the interaction between cells and their extracellular matrix within the periodontium. (health.am)
- During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
- The individual cells within a single-cell layer are physically attached to the matrix, and when displaced from it, they promptly undergo programmed cell death. (elifesciences.org)
- Moreover, cancerous cells tend to cluster together when they are detached from the extracellular matrix. (elifesciences.org)
- Moreover, PVRL4 triggers a form of signaling between the cells called integrin β4 signaling that allows them to survive without being anchored to the extracellular matrix. (elifesciences.org)
- This process is marked by a complex and coordinated set of molecular changes leading to the motile behavior of the invading cancer cells, which involves dynamic cytoskeletal changes, cell-matrix interactions, localized proteolysis, actin-myosin contractions, and focal contact disassembly. (medscape.com)
Internal limiting1
- One potential limit to the efficacy of intravitreal viral injection stems from the physical barriers formed by the vitreous, internal limiting membrane (ILM), retinal extracellular matrix (ECM), and cell surface proteoglycans. (molvis.org)
Tissues2
- Basement membranes (BMs) are thin, dense sheets of extracellular matrix found covering most tissues in multicellular organisms. (duke.edu)
- A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
Cytoplasmic1
- The staining of the membrane, cytoplasmic and stroma was assessed according to intensity. (cancerindex.org)
Glycoproteins1
- Laminins are a family of glycoproteins of the extracellular matrix of all animals. (wikipedia.org)
Lipid1
- In an effort to elucidate the mechanism of bacterial killing by PBP 10, we compared its effects on artificial lipid bilayers and eukaryotic cell membranes with the actions of the mellitin, magainin II, and LL37 peptides. (upenn.edu)
Islet2
- Here, we show that pericytes produce multiple components of the mouse pancreatic and islet interstitial and BM matrices. (nature.com)
- Topics covered include matrix and receptor compositions in native islets, effects of isolation and culture on islet-ECM interactions, and potential for postisolation restoration of islet-ECM interactions. (northwestern.edu)
Metalloproteinase1
- Matrix Metalloproteinase 9. (bvsalud.org)
Retinal1
- To determine whether the co-injection of extracellular matrix degrading enzymes improves retinal transduction following intravitreal delivery of adeno-associated virus-2 (AAV2). (molvis.org)
Interactions2
- These data may indicate altered cell-extracellular matrix interactions in diabetes. (nih.gov)
- Dr. Kenneth Yamada's research focuses on discovering novel mechanisms and regulators of cell interactions with the extracellular matrix and their roles in craniofacial development and disease. (nih.gov)
Fibronectin1
- Basement membrane regulates fibronectin organization using sliding focal adhesions driven by a contractile winch. (nih.gov)
Vitro2
- To determine if the abnormal basement membrane in diabetes has altered epithelial recognition of sites for hemidesmosome formation and if diabetic epithelium can recognize sites for hemidesmosome formation on normal basement membrane, we recombined epithelial sheets and corneal stromas with denuded basement membranes in vitro using diabetic and control corneas. (nih.gov)
- 1998. Protection by extracellular glutathione against sulfur mustard induced toxicity in vitro. (cdc.gov)
Integrin2
- In muscle, it binds to alpha-dystroglycan and integrin alpha7-beta1 via the G domain, and via the other end, it binds to the extracellular matrix. (wikipedia.org)
- PVRL4 promotes anchorage-independence by driving cell-to-cell attachment and matrix-independent integrin β4/SHP-2/c-Src activation. (elifesciences.org)
Invasion3
- Perforating the basement membrane is a cancer cell's first move toward invasion, but how? (medindia.net)
- The metastatic process involves sequential steps, including invasion through the surrounding extracellular matrix (ECM) and stromal cell layers, intravasation into the lumina of blood vessels, and survival in the circulation. (medscape.com)
- One of the most basic features is cell invasion and movement through the extracellular matrix. (medscape.com)
Accumulation1
- Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)
Vascular1
- Small Diameter Xenogeneic Extracellular Matrix Scaffolds for Vascular Applications. (mayo.edu)
Perlecan1
- Perlecan has multiple functions, including cell signaling and the normal maintenance of basement membranes and cartilage. (medlineplus.gov)
Alterations1
- Microhematuria and subsequently proteinuria are hallmarks of kidney involvement, which are due to primary basement membrane alterations that mainly cause endothelial thrombosis and podocyte contraction and ulterior irreversible detachment. (hindawi.com)
Tumors1
- The basement membrane is also the frontline physical barrier that keeps primary tumors from spreading into the matrix below. (medindia.net)
Fibroblasts1
- But there are also carcinoma-associated fibroblasts (CAF) whose matrix proteinases could come in handy in breaking the membrane barrier. (medindia.net)
Morphogenesis1
- Patterned cell and matrix dynamics in branching morphogenesis. (nih.gov)
Corneal1
- The basement membrane is also important for epithelial migration, and abnormalities in basement membrane structure, whether due to trauma (eg, recurrent erosion syndrome) or dystrophy (eg, basement membrane dystrophy), can lead to persistence of corneal epithelial defects and stromal ulceration. (medscape.com)
Fibers1
- and ( 3 ) ineffective repair of elastic fibers and perhaps other extracellular matrix components. (atsjournals.org)
Components1
- PBP 10, an antibacterial, cell membrane-permeant rhodamine B-conjugated peptide derived from the polyphosphoinositide binding site of gelsolin, interacts selectively with both lipopolysaccharides (LPS) and lipoteichoic acid (LTA), the distinct components of gram-negative and gram-positive bacteria, respectively. (upenn.edu)
Bacterial1
Found1
Defects1
- Defects involve fusion of adjacent lobules, disrupted layering of neurons and glia, and fragmentation of the pial basement membrane. (jneurosci.org)
Involvement1
- Therefore, we examined the involvement of the extracellular matrix in this process. (rupress.org)
Thin1
- HN - 2008 BX - Lateral Sinus MH - Atrial Septum UI - D054087 MN - A07.541.459.249 MS - The thin membrane-like muscular structure separating the right and the left upper chambers (HEART ATRIA) of a heart. (bvsalud.org)
Assembly1
- Erhard Hohenester is a structural biologist investigating the assembly, structure, and function of extracellular matrix. (imperial.ac.uk)