• Methods This prospective, observational study in a surgical ICU of a French tertiary hospital reports mHLA-DR measurements (fixed flow cytometry protocol) done 1st within the 3 days post-admission and 2nd after the 7th day. (medrxiv.org)
  • At 1 week, cells were analyzed by means of flow cytometry for cell-surface marker expression (HLA-DR, CD80, CD86, Toll-like receptor 2, Toll-like receptor 4, mCD14, and CD16), phagocytosis (IgG-opsonized zymosan particles), and intracellular killing of Streptococcus pneumoniae. (cdc.gov)
  • Flow Cytometry: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Flow Cytometric Analysis of Human Raji cells using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
  • Through the development of an anti-ICAM1 antibody against the newly found epitope, he succeeded in inducing antigen-specific T cell tolerance via the development of tolerogenic semi-mature dendritic cells (J Exp Med. (wikipedia.org)
  • 6 With respect to activity, HLA-G molecules can inhibit natural killer cell mediated and antigen specific CD8 + T cell mediated cytolysis, 7 induce apoptosis of activated CD8 + T cells, 8 suppress the proliferation of allogeneic CD4 + T cells, 9 and inhibit the transendothelial migration of natural killer cells. (bmj.com)
  • To perform this function, DC are capable of capturing antigens, processing them, and presenting them on the cell surface complexed to major histocompatibility (MHC) molecules, for example, human leukocyte antigen- (HLA-) DR. Along with that, context-dependent expression of costimulatory molecules, such as CD80 and CD86, and secretion of cytokines occurs. (hindawi.com)
  • This monoclonal antibody reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. (novusbio.com)
  • In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. (novusbio.com)
  • HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). (thermofisher.com)
  • Antigens are the molecules that are recognized and stimulate the cells of immune system. (medscape.com)
  • Immunoglobulins (Igs), the term is sometimes used interchangeably with "antibodies," are glycoprotein molecules produced by B lymphocytes and plasma cells in response to an immunogen or after recognition of specific epitopes on the antigen. (medscape.com)
  • Variable region constitutes the antibody binding region of the molecule to the different antigens as it consists of about 110 amino acids that vary widely among the different antibody molecules. (medscape.com)
  • A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. (bdbiosciences.com)
  • The TU169 monoclonal antibody specifically binds to the human Major Histocompatibility Complex (MHC) Class II HLA-DQ1 and DQ2 antigens and weakly to HLA-DQ3. (bdbiosciences.com)
  • Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. (thermofisher.com)
  • In contrast, interferon-gamma (IFN-gamma), which induced major histocompatibility complex (MHC) class II expression, did not induce IL-1 or TNF alpha production. (ox.ac.uk)
  • The field of the subject invention is the use of major histocompatibility complex antigen lacking cells and organs which may serve as universal donors in cellular and organ therapies including transplantation and to produce chimeric non-human mammals. (justia.com)
  • As part of the system for the self-versus-foreign recognition, the surface membrane protein major histocompatibility complex (MHC) antigens serve an important role. (justia.com)
  • In this study, we have used tetrameric major histocompatibility complex-peptide complexes to directly visualize antigen-specific cluster of differentration (CD)8+ T cells during the primary immune response to Epstein-Barr virus (EBV) infection in humans. (ox.ac.uk)
  • The majority of the antigen-specific cells had an activated/memory phenotype, with expression of human histocompatibility leukocyte antigen (HLA) DR, CD38, and CD45RO, downregulation of CD62 leukocyte (CD62L), and low levels of expression of CD45RA. (ox.ac.uk)
  • 12 - 15 These studies focused mainly on major histocompatibility complex (MHC) class II genes, and found evidence that class II alleles such as HLA-DRB1*03:01 are associated with auto-antibody production. (cdc.gov)
  • Background post-injury acquired immunodepression (AID) is frequently assessed by the diminished expression of Human Leukocyte Antigen-D Related on circulating monocytes (mHLA-DR). The relation with mortality and the occurrence of ICU-acquired infections (IAI) requires confirmation in large cohorts of patients. (medrxiv.org)
  • HLA-DQ is expressed primarily on B cells, monocytes, macrophages, dendritic cells and activated T lymphocytes. (bdbiosciences.com)
  • Sia + IgG induced increased expression of CD80 and dendritic cell immunoreceptor (DCIR) on monocytes, whereas the expression of HLA-DR was decreased. (scirp.org)
  • D. Bruder, E. , O. Richards, J. , M. Michel, K. and Oaks, M. (2016) Differential Effects of Alternative Glycoforms of IgG on Human Monocytes and Macrophages: Sialylated IgG Induces Novel Expression Signatures of Cell Surface Markers, Cytokines, and Chemokines. (scirp.org)
  • HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. (thermofisher.com)
  • Granulocyte-macrophage colony stimulating factor induces both HLA-DR expression and cytokine production by human monocytes. (ox.ac.uk)
  • It has been suggested that the non-classic HLA class I molecule HLA-G plays a role in autoimmune disease by protecting tissues from damage by infiltrating cytotoxic T cells. (bmj.com)
  • Controversy and debate surround the role of the non-classic human lymphocyte antigen (HLA) class I molecule HLA-G. 1, 2 Initially, the expression of HLA-G was reported to occur only in a limited subset of trophoblast cells at the fetal-maternal interface. (bmj.com)
  • Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. (thermofisher.com)
  • We conducted a meta-analysis of four independent European SLE case collections for associations between SLE sub-phenotypes and MHC single-nucleotide polymorphism genotypes, human leukocyte antigen (HLA) alleles and variant HLA amino acids. (cdc.gov)
  • 13 Our study substantially expands this work by not only analysing imputed classical human leukocyte antigen (HLA) alleles, but also examining variant HLA amino-acid positions in conjunction with single-nucleotide polymorphism (SNP) genotypes across the extended MHC region (chromosome 6: 26-34 Mb). (cdc.gov)
  • Throughout his career as a T cell immunologist, Park established the theory of T cell-T cell interaction in human thymus, in which T cells expressing MHC class II drive previously unrecognized types of T cells and provide another significant developmental mechanism of T cells. (wikipedia.org)
  • HLA-G expression was also examined in cultured thyroid follicular cells (TFCs). (bmj.com)
  • 3 However, further studies have suggested that HLA-G is expressed in other tissues such as thymic epithelial cells, 4 in addition to tumours 5 and transplant biopsies. (bmj.com)
  • 10 Coupled with the reported tissue distribution, these inhibitory effects upon immune cells have led to the suggestion that HLA-G has a role in maintaining maternal-fetal tolerance, 11 reducing transplant rejection, 12 and in allowing the progression of tumours. (bmj.com)
  • 16 Such observations have initiated the idea that HLA-G might protect tissues from damage by infiltrating cytotoxic T cells during the process of inflammation 2 because HLA-G has been shown to modulate the T helper (Th) cytokine balance in favour of Th2 type anti-inflammatory cytokine responses. (bmj.com)
  • Interestingly, the number of CD80 + pDC positively correlates with the presence of IL-10-producing regulatory type 1 cells (Tr1), an important cell type for maintaining peripheral tolerance to self-antigens. (hindawi.com)
  • DC are professional antigen-presenting cells with the unique capacity to polarize the differentiation of T cells, thereby regulating the balance between inflammation and tolerance [ 12 ]. (hindawi.com)
  • Anti-HLA-DR is intended for in vitro diagnostic use in the identification of cells expressing the HLA-DR antigen, using a BD FACS™ brand flow cytometer. (bdbiosciences.com)
  • To characterize gene expression signatures in acute lymphocytic leukemia (ALL) cells associated with known genotypic abnormalities in adult patients. (aacrjournals.org)
  • The impacts of proinflammatory cytokines and antigen presentation by pleural mesothelial cells (PMCs) on Th22-cell differentiation were explored. (atsjournals.org)
  • In particular, PMCs were able to function as antigen-presenting cells to stimulate CD4 + T-cell proliferation and Th22-cell differentiation. (atsjournals.org)
  • Pleural mesothelial cells stimulate CD4 + T-cell proliferation and Th22 cell differentiation in response to tuberculosis antigen. (atsjournals.org)
  • Naive CD4 + T cells can develop into various helper T (Th) subsets with different cytokine profiles and play a discriminative role in translating antigen-specific immune responses into tissue functions or immunopathology. (atsjournals.org)
  • We have previously shown that the sialic acid-containing (Sia + ) fraction of intravenous immune globulin (IVIG) influences cell surface marker expression and cytokine/ chemokine secretion during the differentiation and maturation of human dendritic cells (DC). (scirp.org)
  • Cancer/testis antigen MAGEC2 (also known as HCA587) is highly expressed in a wide variety of tumors and plays an active role in promoting growth and metastasis of tumor cells. (biomedcentral.com)
  • However, little is known for the regulation of MAGEC2 expression in cancer cells. (biomedcentral.com)
  • We demonstrate that MAGEC2 interacts with TRIM28 in melanoma cells and MAGEC2 expression in tumor cells depends on the expression of TRIM28. (biomedcentral.com)
  • The expression level of MAGEC2 protein was significantly reduced when TRIM28 was depleted in tumor cells, and no changes were observed in MAGEC2 mRNA level. (biomedcentral.com)
  • Mechanistic studies indicate that the regulatory role of TRIM28 on MAGEC2 protein expression in tumor cells depends on proteasome-mediated pathway. (biomedcentral.com)
  • Our findings show that TRIM28 is necessary for MAGEC2 expression in cancer cells, and TRIM28 may serve as a new potential target for immunotherapy of cancer. (biomedcentral.com)
  • In this study, we found that expression of MAGEC2 protein in tumor cells depends on the expression of TRIM28, a reduction in the level of endogenous TRIM28 expression in melanoma cells resulting in significantly decreased expression of MAGEC2 protein. (biomedcentral.com)
  • L-T4 and T3 also potentiate induction by IFN-γ of MHC class II HLA-DR antigen expression in HeLa cells. (tmu.edu.tw)
  • HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. (novusbio.com)
  • However, IFN-gamma enhanced the cell surface expression of HLA-DR and the production of IL-1 and TNF alpha on monocyte-enriched cells stimulated by GM-CSF. (ox.ac.uk)
  • Direct visualization of antigen-specific CD8+ T cells during the primary immune response to Epstein-Barr virus In vivo. (ox.ac.uk)
  • We show that massive expansion of activated, antigen-specific T cells occurs during the primary response to this virus. (ox.ac.uk)
  • After recovery from AIM, the frequency of antigen-specific T cells fell in most donors studied, although populations of antigen-specific cells continued to be easily detectable for at least 3 yr. (ox.ac.uk)
  • The cellular response is mainly a lymphocyte-mediated reaction, whereas the humoral response includes production of antibodies against the antigen by the plasma cells. (medscape.com)
  • His team treated the monkeys with the anti-ICAM1 epitope antibody before the xenotransplantation process, expecting the induction of immune-tolerance to the pancreatic antigens of other species in the non-human primates. (wikipedia.org)
  • Among the reported biomarkers for the characterization of immune status, the expression of human leucocyte antigen-D related on circulating monocyte (mHLA-DR) has gained credence during the past decade. (medrxiv.org)
  • Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression. (bdbiosciences.com)
  • Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. (bdbiosciences.com)
  • Class II Human Leukocyte Antigen (HLA) play an important role in host protection from foreign. (hla-dr.com)
  • Background: Monocytic human leukocyte antigen DR (mHLA-DR) expression levels have been reported to be a marker of immunosuppression and predictors of sepsis and death. (hla-dr.com)
  • The present study examined the effects of Sia + IgG on human peripheral blood mononuclear cell (PBMC)-derived monocyte and macrophage surface marker expression and cytokine/chemokine secretion. (scirp.org)
  • Furthermore, expression levels of MAGEC2 and TRIM28 are positively correlated in MAGEC2-positive human hepatocellular carcinoma tissues ( p = 0.0011). (biomedcentral.com)
  • Immunohistochemistry-Paraffin: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
  • Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. (thermofisher.com)
  • Gene expression profiling was performed using Whole Human Genome 4x44K Oligonucleotide Microarrays. (biomedcentral.com)
  • The effect of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) on the expression of HLA-DR, and the production of the cytokines interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha) by human peripheral blood monocyte-enriched populations was investigated. (ox.ac.uk)
  • By itself, GM-CSF did not induce surface class II expression on the human monocytic tumour cell line THP-1, whereas it synergized with IFN-gamma to induce surface expression. (ox.ac.uk)
  • Western Blot: HLA DRB1 Antibody (LN-3 + HLA-DRB/1067) [NBP2-45316] - Western Blot Analysis of Ramos cell lysate using HLA DRB1 Antibody (LN-3 + HLA-DRB/1067). (novusbio.com)
  • HLA-DRB1*03:01 was significantly associated with both sub-phenotypes. (cdc.gov)
  • Conditional analyses showed that anti-Ro and anti-La subsets are independently associated with HLA-DRB1*0301, and that the HLA-DRB1*03:01 association with SLE is largely but not completely driven by the association of this allele with these sub-phenotypes. (cdc.gov)
  • Our results provide strong evidence for a multilevel risk model for HLA-DRB1*03:01 in SLE, where the association with anti-Ro and anti-La antibody-positive SLE is much stronger than SLE without these autoantibodies. (cdc.gov)
  • Epitopes are a component of the antigen that are recognized by the immune system and determine whether the cellular or the humoral arm of the immune system shall be activated against that particular antigen. (medscape.com)
  • This antibody does not cross-react with HLA-DR2 and HLA-DR7. (bdbiosciences.com)
  • The following product was used in this experiment: HLA-DR Monoclonal Antibody (LN3), Super Bright™ 436, eBioscience™ from Thermo Fisher Scientific, catalog # 62-9956-42, RRID AB_2744822. (thermofisher.com)
  • HIV infection can be diagnosed by antibody, nucleic acid (HIV RNA), or antigen (p24) testing. (msdmanuals.com)
  • Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. (novusbio.com)
  • Furthermore, HLA-G expression could not be detected in cultured patient TFCs under basal conditions or after stimulation with the proinflammatory cytokines-interleukin 1α, interferon γ, and tumour necrosis factor α. (bmj.com)
  • In macrophage-colony stimulating factor (MCSF) generated macrophages, Sia + IgG induced increased production of numerous cytokines/ chemokines including IL-6, TNFα, CXCL1, and IL-10, and the expression of the macrophage surface marker CD163. (scirp.org)
  • GM-CSF was shown to induce both the expression of HLA-DR and the cytokines IL-1 and TNF alpha in a dose-dependent manner. (ox.ac.uk)
  • In genetically susceptible individuals, this antigenic stimulation may ultimately result in the expression of overt rheumatoid arthritis (RA) via molecular mimicry, a process whereby foreign peptides, similar in structure to endogenous peptides, may cause antibodies or T-lymphocytes to cross-react with both foreign and endogenous peptides and thereby break immunological tolerance. (cambridge.org)
  • The antibodies then specifically bind to only those particular antigens. (medscape.com)
  • It does not cross react with HLA-DP and HLA-DQ. (novusbio.com)
  • We observed that age advancement in all three groups combined was associated with a monocyte immune phenotypic profile related to inflammation and a T cell immune phenotypic associated with immune senescence and chronic antigen exposure. (frontiersin.org)
  • The identified monocyte and T cell immune phenotypic profiles that were associated with age advancement, were strongly related to inflammation, chronic antigen exposure and immune senescence. (frontiersin.org)
  • Supervised analysis of kinase genes revealed a high-level FLT3 expression in a subset of cases without molecular rearrangements. (aacrjournals.org)
  • Interestingly, almost half of the genes was related to the patients' immune response and showed reduced expression in the metastatic cases. (biomedcentral.com)
  • Whereas up to now gene signatures containing genes with various biological functions have been described for prediction of metastasis in CRC, in this study metastasis could be well predicted by a set of gene expression markers consisting exclusively of genes related to the MHC class II complex involved in immune response. (biomedcentral.com)
  • Unlike methods that attempt to select individual genes by their significant differential expression between classes of samples (e.g. (biomedcentral.com)
  • Homologous recombination is employed to inactivate genes, particularly genes associated with MHC antigens. (justia.com)
  • The T cell immune phenotypic profile in blood donors was related to loss of T cell function, whereas the same set of markers were related to chronic antigen stimulation and immune senescence in HIV-negative individuals. (frontiersin.org)
  • CONCLUSION: Repetitive organic dust exposure significantly decreases markers of antigen presentation and host defense function in MDMs. (cdc.gov)
  • Sia + IgG also increased the expression of cell surface markers associated with macrophage polarization (e.g. (scirp.org)
  • This study aimed at the identification of prognostic gene expression markers in early primary colorectal carcinomas without metastasis at the time point of surgery by analyzing genome-wide gene expression profiles using oligonucleotide microarrays. (biomedcentral.com)
  • HLA-DQ antigens exits as heterodimers comprised of polymorphic transmembrane HLA-DQ alpha and beta glycoproteins. (bdbiosciences.com)
  • HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. (thermofisher.com)
  • Thus, PKA and PKC have discrete roles in IFN-γ-induced MHC class II antigen expression and its modulation by thyroid hormone. (tmu.edu.tw)
  • sub.2 -microglobulin gene is inactivated for reducing or eliminating Class I MHC antigens. (justia.com)
  • Each host has a personal set of Class I and II MHC antigens, which serve to distinguish that host from other hosts. (justia.com)
  • HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. (novusbio.com)
  • Unravelling an HLA-DR association in childhood acute lymphoblastic leukemia. (bdbiosciences.com)
  • In such a situation, specialized methods of analysis for differential gene expression are required. (biomedcentral.com)
  • In the present review, we provide evidence for how the interaction of dietary lectins with enterocytes and lymphocytes may facilitate the translocation of both dietary and gut-derived pathogenic antigens to peripheral tissues, which in turn causes persistent peripheral antigenic stimulation. (cambridge.org)
  • The aim of the current study was to analyse thyroid tissue and thyrocytes obtained from individuals with autoimmune thyroid disease for the expression of HLA-G. (bmj.com)
  • HLA-G expression was analysed in thyroid tissue taken from six patients with GD and one with HT by reverse transcriptase polymerase chain reaction. (bmj.com)
  • The expression of HLA-G was not detected in the thyroid gland of patients with either GD, HT, or MNG. (bmj.com)
  • HLA-G expression does not occur in the thyroid of patients with GD, indicating that HLA-G does not play a pathophysiological role in this autoimmune disorder. (bmj.com)
  • Although the expression of HLA-G was not detected in the thyroid sample of the patient with HT, a greater sample size would be required to conclude that HLA-G does not have a part to play in this autoimmune thyroid disease. (bmj.com)
  • In the presence of IFN-γ and 10-7 M T4, the same concentrations of staurosporine inhibited potentiation of HLA-DR expression by thyroid hormone. (tmu.edu.tw)
  • A more specific PKC inhibitor, CGP41251 (0.5-5 nM), similarly enhanced HLA-DR expression in the presence of IFN-γ but inhibited thyroid hormone potentiation of antigen expression. (tmu.edu.tw)
  • BCR/ABL + gene expression pattern was more heterogeneous and was most similar to ALL without known molecular rearrangements. (aacrjournals.org)
  • PGN, LPS, and PGN plus LPS significantly reduced MDM surface marker expression and, except for LPS alone, also reduced phagocytosis. (cdc.gov)
  • The associations between mHLA-DR and outcomes were tested by adjusted Fine and Gray sub-distribution competing risk models. (medrxiv.org)
  • Our data indicate that the Sia + fraction of IVIG possesses the ability to influence inflammatory processes in multiple immune cell types and induces novel signatures in cell surface marker expression and cytokine/chemokine production. (scirp.org)
  • The persistence or a decrease of low mHLA-DR expression are independent and reliable predictors of ICU-acquired infection. (medrxiv.org)
  • Leucocyte Typing IV: White Cell Differentiation Antigens. (bdbiosciences.com)
  • Moreover, PMCs were able to stimulate CD4 + T-cell proliferation and Th22-cell differentiation by presenting tuberculosis-specific antigen. (atsjournals.org)
  • Another important achievement of his research is the induction of antigen-specific T cell tolerance, which has been a distant dream of immunologists for pinpoint-targeted control of the immune system. (wikipedia.org)
  • The magnitude of the antigen-specific component versus the bystander component of a primary T cell response remains controversial. (ox.ac.uk)
  • Our aim was to discover genetic loci within the MHC region that are associated with specific clinical and/or immunological manifestations within SLE cases and hence to find evidence of genetic variants that may drive specific forms of the disease. (cdc.gov)
  • To our knowledge, this is the first time to report the role of TRIM28 in regulating the expression of cancer/testis antigen MAGEC2. (biomedcentral.com)
  • Gene expression profiles from 128 adult patients with newly diagnosed ALL were characterized using high-density oligonucleotide microarrays. (aacrjournals.org)
  • Overexpression and knockdown assays were used to examine the effects of TRIM28 on the expression of MAGEC2 protein. (biomedcentral.com)
  • The potentiating effect of T4 was much more sensitive to a cyclic AMP-dependent protein kinase (PKA) inhibitor, KT5720 (1-1000 nM), than was the induction of HLA-DR by IFN-γ. (tmu.edu.tw)
  • therefore, GM-CSF may be of importance in potentiating antigen presenting function. (ox.ac.uk)
  • A phospholipase C inhibitor, U73122 (1-1000 nM), did not alter the potentiating ability of T4, although it inhibited in a concentration- dependent manner the expression of HLA-DR induced by IFN-γ. (tmu.edu.tw)
  • The body's immune system can react adaptively against the antigen via 2 pathways: cellular or humoral. (medscape.com)
  • The lymphoid system is predicated upon recognition of the presence of such MHC antigens as self. (justia.com)
  • In the present studies of HLA-DR expression, the PKC inhibitor staurosporine (0.1-1 nM) enhanced the expression of HLA-DR when the inhibitor was added simultaneously with IFN- γ, 100 IU/ml. (tmu.edu.tw)