• In the present study, polymerase chain reaction and western blot analyses were performed to examine the expression levels of miR‑130a and associated proteins. (spandidos-publications.com)
  • Adipose tissue plays an important role in metabolism through its storage and release of triglycerides, peptide hormones (adipokines) and other proteins, and in the case of brown fat, for its role in thermogenesis ( 1 ). (diabetesjournals.org)
  • The team used a hybrid method that blended tests that read the order of DNA bases with those that analyze gene expression, the process by which genes make cellular proteins. (uclahealth.org)
  • A known combination marker of cancer stem cells is high expression of CD44 and low expression of CD24 surface proteins. (weeksmd.com)
  • The putative targets were cell-surface proteins (or proteins with extracellular epitopes) highly expressed in neuroblastoma compared with normal tissues, and expressed in the majority of tumors. (aacrjournals.org)
  • One clue to this dilemma has emerged from the identification of "targeting" subunits that allow for the compartmentalized activation of the phosphatase, resulting in the specific dephosphorylation of discrete pools of proteins within the cell. (jci.org)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • Cadherin genes encode a superfamily of calcium-dependent adhesion-related type-1 transmembrane proteins. (biomedcentral.com)
  • GIP receptor deletion in mice confers resistance to high-fat diet-induced obesity via alterations in energy expenditure and adipose tissue lipid metabolism. (nih.gov)
  • These mice have reduced glycogen stores in adipose tissue, liver, heart, and skeletal muscle, corresponding with decreased glycogen synthase activity and glycogen synthesis rate. (jci.org)
  • By directing AAV transduction through the regulated expression of its receptor, the approach provides precise control over which cells express the AAV-vectored transgene. (nature.com)
  • Furthermore, the expression of miR‑130a was also higher in HPV(+) cervical cancer cell lines compared with that in HPV(‑) cells. (spandidos-publications.com)
  • Knockdown of HPV18 E6 significantly inhibited the expression of miR‑130a in HeLa cervical cancer cells. (spandidos-publications.com)
  • The expression of TIMP2 was negatively mediated by miR‑130a, and HPV18 E6 inhibited the expression of TIMP2 in HeLa cells. (spandidos-publications.com)
  • Zhou Y, Yang C, Wang K, Liu X and Liu Q: MicroRNA-33b inhibits the proliferation and migration of osteosarcoma cells via targeting hypoxia-inducible factor-1α. (spandidos-publications.com)
  • Wang S, Hui Y, Li X and Jia Q: WITHDRAWN: Silencing of lncRNA-CCDC26 restrains the growth and migration of glioma cells in vitro and in vivo via targeting miR-203. (spandidos-publications.com)
  • The Adipoq-Cre exhibited no observable recombination in any other tissues examined, whereas both aP2-Cre lines resulted in recombination in endothelial cells of the heart and nonendothelial, nonmyocyte cells in the skeletal muscle. (diabetesjournals.org)
  • Brain cells and genes related to synaptic function are expressed in the blood, offering a window into gene expression. (uclahealth.org)
  • Cancer stem cells are similar to normal stem cells that renew specialized tissues. (weeksmd.com)
  • They found that GD2 expression, one such mesenchymal stem cell marker, divided the breast cancer cell lines into two distinct groups: about 4.5 percent of cells were GD2-positive and about 92.7 percent were GD2-negative. (weeksmd.com)
  • Comparing gene expression between GD2+ cells and CD44 high/CD24 low cells revealed 100 percent correlation in the expression of 231 genes. (weeksmd.com)
  • High-throughput sequencing methods are extremely powerful tools to quantify gene expression in bulk tissue and individual cells. (umd.edu)
  • Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
  • Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. (cshlpress.com)
  • Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
  • The dependency of neuroblastoma cells on GPC2 expression may prevent the emergence of immune escape mechanisms in response to therapeutic targeting. (aacrjournals.org)
  • A GPC2-targeting antibody-drug conjugate was developed and was cytotoxic to neuroblastoma cells expressing GPC2 in vitro . (aacrjournals.org)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
  • The study of single cells within their native tissue environment, known as spatial biology, helps scientists better characterize and understand disease. (enzo.com)
  • Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
  • The content of MCP-1, TNF-ɑ, and ICAM-1, the expression of HMGB1 and NF-κB p65 , apoptotic cells counting and tissue damage in the model group and agomir-NC group were found to be all higher than those in the control group. (magtechjournal.com)
  • Anti-FBG antibodies inhibited cytokine release by RA synovial cells and prevented disease progression and tissue destruction during collagen-induced arthritis. (bmj.com)
  • these antibodies inhibit cytokine release by RA synovial cells and prevent disease progression and tissue destruction during collagen-induced arthritis. (bmj.com)
  • However, the expression and function of PD-1 in colon cancer cells has not been reported. (jcancer.org)
  • We also demonstrated that high concentrations of C. tropicalis can down-regulate tumor cell-intrinsic PD-1 expression in colon cancer cells. (jcancer.org)
  • Mechanistically, we found that C. tropicalis modulates the expression of PD-1 via increasing the autophagy traffic in colon cancer cells. (jcancer.org)
  • C. tropicalis can down-regulate tumor cell-intrinsic PD-1 expression via enhancing tumor cells autophagy levels to promote CRC progression. (jcancer.org)
  • Quercetin also improve the action of the drug 5-Fluorouracil (5-FU) promoting increased expression of p53 and apoptosis in breast cancer T47D cells [ 6 , 7 ]. (biomedcentral.com)
  • In the study presented at SITC, Ziopharm's non-viral gene transfer technology, Sleeping Beauty, was used to develop TCR-T cells targeting EGFR, KRAS and p53 neoantigens, which are present in cancer cells but not in normal tissue, and are presented by a variety of human leukocyte antigen (HLA) molecules on the cancer cell surface. (archiebronsonoutfit.net)
  • Ziopharm's Sleeping Beauty technology exhibited greater than 60% expression of the introduced neoantigen-specific TCRs in the generated TCR-T cells. (archiebronsonoutfit.net)
  • However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
  • This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
  • Wang C, Zhou B, Liu M, Liu Y and Gao R: miR-126-5p restoration promotes cell apoptosis in cervical cancer by targeting Bcl2l2. (spandidos-publications.com)
  • CRT effects are related to alterations in genes and microRNAs (miRs) expression, which regulate cardiac processes involved in cardiac apoptosis, cardiac fibrosis, cardiac hypertrophy and angiogenesis, and membrane channel ionic currents. (hindawi.com)
  • miR-140-5p affects chondrocyte proliferation, apoptosis, and inflammation by targeting HMGB1 in osteoarthritis[J]. Inflamm Res, 2020, 69:63-73. (magtechjournal.com)
  • Immunotherapeutic GPC2 targeting may be beneficial in high-risk neuroblastoma and other embryonal tumors. (aacrjournals.org)
  • MYCN bound to the GPC2 promoter to increase its expression in neuroblastoma, thereby upregulating GPC2 in MYCN -amplified tumors. (aacrjournals.org)
  • GPC2 was highly expressed on the plasma membrane of the majority of neuroblastoma tumors, and its expression was limited in normal tissues, suggesting the possibility for therapeutic targeting with minimal toxicity. (aacrjournals.org)
  • miR-149 is a type of miRNA, which can regulate the biological functions of tumors by targeting multiple genes, such as TGF- β 2, GIT1, and HDAC4 [ 7 - 9 ]. (hindawi.com)
  • Mice develop tumors resembling those seen in humans, including expression of high levels of miR-21. (genengnews.com)
  • This antibody, a modified version of 3E10, forms non-covalent complexes with RNAs and can mediate highly specific delivery broadly into tumors via intravenous injection by targeting the nucleoside transporter ENT2. (biospace.com)
  • A luciferase reporter gene assay was performed to confirm the targeting associations of miR‑130a. (spandidos-publications.com)
  • Dual luciferase reporter gene assay was used to detect the targeting relationship between miR-140-5p and HMGB1. (magtechjournal.com)
  • miRNAs play an important role in regulating gene expression by binding with 3 - UTR of the target gene. (hindawi.com)
  • MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides, which are key regulators of gene expression [ 4 ]. (hindawi.com)
  • Therefore, investigating the expression and regulation of miRNAs may elucidate the mechanism of gastric cancer. (hindawi.com)
  • Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
  • The involvement of miRNAs in cancer research emerged from studies demonstrating their aberrant expression in neoplastic tissues. (genengnews.com)
  • Transcriptomics has also expanded from sampling protein coding genes to non-coding transcripts such as miRNAs, which have the potential not only to act as more stable biomarkers, but also to regulate translation, and in themselves be used a therapeutic agents against specific targets. (videolectures.net)
  • MicroRNAs (miRNAs) constitute a class of short noncoding RNAs which regulate gene expression by targeting messenger RNA, inducing translational repression and messenger RNA degradation. (aston.ac.uk)
  • Hypergeometric analysis was used to identify canonical pathways and curated networks having statistically significant enrichment of differentially expressed genes. (urotoday.com)
  • The highest number of differentially expressed transcript clusters was found when week 1 and week 2 old scleral tissues were compared. (molvis.org)
  • The peroxisome proliferator- activated receptor gamma coactivator 1-alpha ( Ppargc1a ) gene was found to be involved in the networks generated using Ingenuity Pathway Studio (IPA) from the differentially expressed transcript cluster lists of week 2 versus 1, week 3 versus 2, week 6 versus 3, and week 8 versus 6. (molvis.org)
  • Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
  • These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)
  • Gene targeting of chondromodulin-I resulted in enhanced VEGF-A expression, angiogenesis, lipid deposition and calcification in the cardiac valves of aged mice. (wikipedia.org)
  • Transgene expression using viral vectors provides an alternative, which allows for temporal expression of genes in mice with reduced cost and effort. (nature.com)
  • The system allows for the generation of mice with specific overexpression of AAVR through breeding with available Cre mouse lines, as well as the option to detarget AAV from other tissue when paired with Aavr -KO mice. (nature.com)
  • We generated mice that possess a heterozygous deletion of the PTG gene. (jci.org)
  • To address this and to also study the oncogenic potential of unregulated function of dominant regulators of epithelial homeostasis noted above, we developed Multi-Functional Human Tissue Genetics noted above which, when combined with skin tissue regeneration on immune deficient mice, has permitted the molecular reconstruction of events sufficient to trigger human cancer. (stanford.edu)
  • In normal mice, the selected formula exhibited improved bioavailability and liver targeting efficiency compared to raw CUR. (ingentaconnect.com)
  • In conclusion, CUR-ZN nanospheres demonstrated improved liver targeting efficiency and antifibrotic activity in comparison to raw CUR in CCl 4 -induced liver fibrosis in mice. (ingentaconnect.com)
  • PCDH20 mRNA and protein expression is significantly downregulated in the colonic epithelium of Crohn's disease patients and mice with induced colitis compared with controls. (biomedcentral.com)
  • Specifically, the loss of PCDH20 impairs intestinal barrier function by unzipping adherens junctions in mice with colitis via targeting the ATF6/CHOP/β-catenin/p120-catenin axis. (biomedcentral.com)
  • At the end of 21 weeks of the experiment, mice were sacrificed, colon tissue was removed, and then subjected to ELISA, flow cytometry, real-time PCR and histology examination. (biomedcentral.com)
  • The expression of mRNA p53 was up regulated in mice received DPE 125 mg/kg BW. (biomedcentral.com)
  • 6 ], the transforming growth factor beta 1 ( Tgfβ1 ) gene and several collagen genes were significantly downregulated in 8-week-old B6 mice scleras when compared with 3-week-old B6 mice scleras. (molvis.org)
  • These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)
  • The CRT-induced "reverse molecular remodeling" in responders patients is related to an increase in the expression of genes involved in the regulation of excitation-contraction coupling and a reversal in the isoforms switching of the contractile genes [ 9 ] ultimately leading to remodeling effects and improving of myocardial performance. (hindawi.com)
  • Reducing the levels of the H3K4 demethylase SMCX/Jarid1c in primary neurons reversed down-regulation of key neuronal genes caused by mutant Huntingtin expression. (nih.gov)
  • In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
  • Conclusions Up-regulation of miR-140-5p may negatively regulate HMGB1 and improve the expression of IκB-α to alleviate retinopathy of diabetic rats. (magtechjournal.com)
  • Not all mutations will influence gene expression, but this approach improves our ability to pinpoint those that do. (uclahealth.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Mutations in the NEU1 gene cause sialidosis. (medlineplus.gov)
  • Mutations in the NEU1 gene lead to a shortage (deficiency) of the NEU1 enzyme. (medlineplus.gov)
  • which means both copies of the gene in each cell have mutations. (medlineplus.gov)
  • If this is the case, gene-correction presents a solution for patients with known monogenetic mutations. (lu.se)
  • Published in the June 21 online edition of the American Journal of Human Genetics, the findings could help scientists zero in on genes that offer future therapeutic targets for the disorder. (uclahealth.org)
  • Several groups, including ourselves have now identified drugs targeting this pathway and it remains a viable target for therapeutic intervention. (videolectures.net)
  • With RNA-sequencing now becoming more affordable and with the advantages of identifying novel transcripts and detecting a greater range of expression levels, the future for gene expression profiling includes focusing on transcripts in specific cellular compartments, being an integral part of clinical trials and perhaps even a standard part of diagnostic procedures and therapeutic decisions. (videolectures.net)
  • The GMAB technology platform, exclusively licensed from Yale University, utilizes a proprietary, cell-penetrating antibody to non-covalently bind to and deliver therapeutic levels of multiple types of nucleic acids, including synthetic RNA (ribonucleic acid) messenger RNA, small interfering RNA, deoxyribonucleic acids, antisense oligonucleotides and gene editing molecules. (biospace.com)
  • Targeted expression of synapto-pHluorin in mouse will permit the analysis of previously inaccessible neuronal populations and chronic imaging from genetically identified neurons in vivo. (nih.gov)
  • Genetic perturbations of photopigment-driven neuronal activity led to global tissue transcriptomic shifts in both the retina and brain targets, but the ipRGC axonal-specific translatome was unaltered. (umd.edu)
  • Dr Tamara Jamaspishvili is a research pathologist, translational research scientist focusing on developing, evaluating, validating, and applying tissue-based prognostic and predictive biomarkers and models for improved disease prognostication and management of cancer patients. (upstate.edu)
  • In light of the advancements in tumor biology and molecular technology, further research into the molecular mechanism involved in gastric cancer and identification of biomarkers with high specificity and good targeting [ 3 ] is of great significance for the diagnosis and treatment of this condition. (hindawi.com)
  • The results of these studies have been used to investigate disease mechanisms, monitor progression of disease and identify specific genes or gene signatures which could be used as diagnostic or prognostic biomarkers. (videolectures.net)
  • Within the field of ALS, researchers have moved from using pooled spinal cord homogenates, to ensure they had sufficient material, through to isolating individual cell types from post-mortem cases and utilising peripheral tissues to determine biomarkers of disease and progression or stratification of patients. (videolectures.net)
  • The researchers used the combined method to prioritize gene targets that merit closer investigation. (uclahealth.org)
  • Protein targeting to glycogen (PTG) is a scaffolding protein that targets protein phosphatase 1α (PP1α) to glycogen, and links it to enzymes involved in glycogen synthesis and degradation. (jci.org)
  • Our goal was to examine gene transcription by B. burgdorferi following doxycycline treatment in an effort to identify both persister-associated genes and possible targets for antimicrobial intervention. (frontiersin.org)
  • To understand the molecular-genetic basis of functional specialization and identify potential drug targets specific to each neuron subtype, we performed a genome wide assessment of both gene expression and splicing across EXC, PV, SST and VIP neurons from male and female mouse brains. (jneurosci.org)
  • This resource, combining a unique new dataset and novel application of analysis methods to multiple relevant datasets, identifies numerous potential drug targets for manipulating circuit function, reveals neuron subtype-specific roles for disease-linked genes, and is useful for understanding gene expression changes observed in human patient brains. (jneurosci.org)
  • Many Cre transgenes have expression in unexpected organs and tissues, even when the promoters are thought to be tissue-specific. (jax.org)
  • Sialidosis is a severe inherited disorder that affects many organs and tissues, including the nervous system. (medlineplus.gov)
  • We investigated whether targeting proinflammatory signals from the extracellular matrix that persist during pathological inflammation provides a viable strategy to treat rheumatoid arthritis (RA). (bmj.com)
  • To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin (Adipoq) gene promoters, as well as a tamoxifen-inducible Cre driven by the aP2 gene promoter (iaP2), were bred to the Rosa26R (R26R) reporter. (diabetesjournals.org)
  • It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (nih.gov)
  • We observed gene-specific changes in histone H3 lysine 4 trimethylation (H3K4me3) at transcriptionally repressed promoters in R6/2 mouse and human HD brain. (nih.gov)
  • We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
  • The expression levels from some promoters can be high enough to affect physiology of the cell. (jax.org)
  • We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
  • Here we describe a model system, SELective Expression and Controlled Transduction In Vivo (SELECTIV), that enables efficient and specific expression of transgenes by coupling adeno-associated virus (AAV) vectors with Cre-inducible overexpression of the multi-serotype AAV receptor, AAVR. (nature.com)
  • The enhanced efficacy and exquisite specificity of SELECTIV has broad utility in development of new mouse model systems and expands the use of AAV for gene delivery in vivo. (nature.com)
  • Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. (diabetesjournals.org)
  • Additionally, we've made progress in hepatocyte-targeted in vivo gene silencing by modifying the siRNAs. (genengnews.com)
  • Here, we applied a high-resolution temporal multiomic analysis during microbially induced inflammation in order to characterize the effects of localized oral inflammation on distant healthy tissues in young healthy adults. (bvsalud.org)
  • Our results provide mechanistic insight into how healthy tissues within humans are influenced by distant localized inflammation and may ultimately become susceptible to disease. (bvsalud.org)
  • Studies completed with labeled RNAs and messenger RNAs expressing a green fluorescent protein reporter gene show tumor specific delivery and functional expression by imaging, with minimal delivery to healthy tissues. (biospace.com)
  • Here, we have targeted expression of synapto-pHluorin, a pH-sensitive protein that reports synaptic vesicle fusion, to olfactory sensory neurons in mouse. (nih.gov)
  • We provide a searchable web resource for exploring differential mRNA expression and splice form usage between excitatory, PV, SST, and VIP neurons ( http://research-pub.gene.com/NeuronSubtypeTranscriptomes ). (jneurosci.org)
  • We sorted excitatory neurons and key inhibitory neuron subtypes from mouse brains and assessed differential mRNA expression. (jneurosci.org)
  • Characterisation of gene expression patterns in bladder cancer allows the identification of pathways involved in its pathogenesis, and may stimulate the development of novel therapies targeting these pathways. (urotoday.com)
  • Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)
  • The ongoing annotation of these genomes should spark a new wave of interest in the DED pathosystem, as it should lead to the formal identification of genes modulating parasitic fitness. (sisef.it)
  • Of 60 blood cultures positive for Gram-positive cocci in clusters, LAMP (via detection of the Fem A and Mec A genes) showed 100% sensitivity and specificity for identification of MRSA/MSSA. (who.int)
  • En parallèle, une identification microbiologique normalisée et un test de sensibilité à l'oxacilline par PCR visant l'amplification du gène Mec A ont été réalisés. (who.int)
  • Limitations in the usage of AAV in murine models include cell types that are refractory to transduction and difficulties achieving tissue-specificity. (nature.com)
  • The sclera is a fibrous connective tissue consisting of 90% collagen and a small percentage of proteoglycans and glycoproteins. (molvis.org)
  • This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
  • The aim of this study was to identify the genes and pathways underlying the growth of the mouse sclera during postnatal development. (molvis.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • orthogonal exPeriMental changes and pathways induced by EGE investigated the impact of aSSeSSMent of ePigenetic environmental factors in cancer causation, both current H. pylori infection and driver geneS and their link to underpinning studies of etiology e , pigenetic memory of past (eradicated) environMental carcinogenS carcinogen evaluation, and prevention. (who.int)
  • Our approach exploits the absolute dependence of AAV transduction on the expression of its receptor 22 , AAVR (also named KIAA0319L). (nature.com)
  • Gene expression profiling of ErbB receptor and ligand-dependent transcription. (rochester.edu)
  • The test group also showed significant reductions in GCF levels of inflammatory markers (matrix metalloproteinase 8 [MMP8], receptor activator of nuclear factor kappa-Β ligand [RANKL]), oral polymorphonuclear neutrophil (PMN) counts, and systemic neutrophil priming (CD11b expression) during experimental gingivitis. (bvsalud.org)
  • These results reveal numerous examples where neuron subtype-specific gene expression, as well as splice-isoform usage, can explain functional differences between neuron subtypes, including in presynaptic plasticity, postsynaptic receptor function, and synaptic connectivity specification. (jneurosci.org)
  • [ 12 ] They found that the glutamate receptor gene GRM1 recombines with several partner genes through promoter swapping and gene fusion. (medscape.com)
  • This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. (wikipedia.org)
  • This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. (nih.gov)
  • This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. (cancerindex.org)
  • It was observed that miR‑130a was significantly upregulated in cervical cancer tissues compared with that in adjacent non‑tumorous tissues. (spandidos-publications.com)
  • High expression of miR‑130a was significantly associated with lymph node metastasis and an advanced clinical stage of cervical cancer. (spandidos-publications.com)
  • When we looked at genes associated with nervous-system function we found significantly more genes were expressed at higher or lower levels in the children diagnosed with autism than we did in their siblings unaffected by the disorder," said Geschwind. (uclahealth.org)
  • Compared to raw CUR, CUR-ZN was significantly more effective in attenuating the rise in hepatic gene expression of collagen-1, tissue inhibitor of metalloproteinase-2, and transforming growth factor beta, as well as the downregulation of matrix metalloproteinase-2 expression. (ingentaconnect.com)
  • Their search turned up new regions in the genome where genetic variations showed strong links to autism and altered expression patterns. (uclahealth.org)
  • jointly fitting multiple samples, targeting genes with consistent spatial patterns across replicates. (bioconductor.org)
  • Using a knock-in Cre also allows you to evaluate the likelihood of any unexpected expression by first reviewing the expression patterns of the endogenous gene. (jax.org)
  • The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
  • In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
  • Approximately 3% of the human genome codes for miRNA sequences, which can suppress gene expression by mediating translational repression [ 5 ]. (hindawi.com)
  • Tissue inhibitor of metalloproteinase 2 (TIMP2), an antagonist of matrix metalloproteinase 2 (MMP2), was identified as a novel, direct target gene of miR‑130a. (spandidos-publications.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • In the case of a GalNAc-conjugated siRNA targeting transthyretin (TTR), target gene silencing was achieved with an ED50 of approximately 5 mg/kg with a single subcutaneous injection. (genengnews.com)
  • These results represent a greater than 30-fold improvement in target gene silencing with siRNA conjugates as compared with first-generation siRNA conjugates previously described. (genengnews.com)
  • It reportedly delivers a greater than 30-fold improvement in target gene silencing. (genengnews.com)
  • Results HMGB1 was the target gene of miR-140-5p. (magtechjournal.com)
  • Here, we identified a PPARγ/microRNA-223 (miR-223) regulatory axis that controls macrophage polarization by targeting distinct downstream genes to shift the cellular response to various stimuli. (jci.org)
  • Experimental designs often aim to quantify expression shifts to characterize developmental trajectories, disease states, or cellular drug responses. (umd.edu)
  • Cell culture studies show antibody-mediated delivery of a series of retinoic acid-inducible gene I (RIG-I) ligands, including hairpin RNAs and synthetic double-stranded RNA (poly(I:C)), resulting in robust RIG-I stimulation and induction of type-1 interferon signaling. (biospace.com)
  • Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
  • This gene is expressed in the avascular zone of prehypertrophic cartilage, and its expression decreases during chondrocyte hypertrophy and vascular invasion. (wikipedia.org)
  • Their appearance can mimic that of fibrous tissue or hyaline cartilage. (medscape.com)
  • GALNS deficiency induces the accumulation of glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) in multiple tissues, particularly bone, cartilage, heart valves, and cornea, whereas β-galactosidase deficiency induces the accumulation of only KS in those tissues. (medscape.com)
  • Based on pre-annotated spatial clusters as summarized spatial information, DESpace models gene expression using a negative binomial (NB), via edgeR, with spatial clusters as covariates. (bioconductor.org)
  • Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. (cdc.gov)
  • Although more and animals and plants and regulate gene/protein expression more progressions have been made about the through direct complementarity between their 5' region pathogenesis of asthma in recent years, the increasing and the 3' untranslated region of target mRNAs. (cdc.gov)
  • Treatment with NMT inhibitor IMP-1088 potently abrogated VACV infection, while VACV gene expression, DNA replication, morphogenesis and EV formation remained unaffected. (cdc.gov)
  • A number of genes were perturbed and most of those which were statistically significant were down-regulated in the treated versus the untreated or treated/re-grown. (frontiersin.org)
  • According to Dr. Marcusson, an important aspect of these studies was the demonstration of miR-21 knockdown using genome-wide mRNA expression studies. (genengnews.com)
  • Imaging of fluorescent protein-labeled Saccharibacteria provides high spatiotemporal resolution of phenomena accompanying epibiotic growth, and a transposon-insertion sequencing (Tn-seq) genome-wide screen reveals the contribution of enigmatic Saccharibacterial genes to growth on their hosts. (bvsalud.org)
  • We used a genome-wide analysis which not only examined differential gene expression levels but could also detect differences in splice isoform usage. (jneurosci.org)
  • A genome-wide gene-environment interaction study of breast cancer risk for women of European ancestry. (medscape.com)
  • Experimental and genetic methods are also rapidly evolving to capture specific aspects of gene expression such as in targeting individual cell types, regulatory stages, and spatially resolved cell subcompartments. (umd.edu)
  • These new models, which we term Multi-Functional Human Tissue Genetics, allow up to 10 alleles or more to be altered simultaneously, permitting genetic experiments with an unprecedented degree of rapidity and complexity. (stanford.edu)
  • This treatment tackles genetic expressions by activating youth factors, thus delaying the skin-aging process for more radiant skin. (psmedical.com.hk)
  • NEW HAVEN, Conn., April 10, 2021 (GLOBE NEWSWIRE) -- Gennao Bio , a privately-held genetic medicines company developing first-in-class, targeted nucleic acid therapeutics, today announced a late-breaking poster detailing preclinical results for its proprietary, non-viral gene monoclonal antibody (GMAB) nucleic acid delivery system. (biospace.com)
  • Gennao Bio is a privately-held genetic medicines company developing first-in-class targeted nucleic acid therapeutics utilizing its proprietary gene monoclonal antibody (GMAB) platform technology. (biospace.com)
  • In summary, the present study suggests that HPV18 E6 promotes the expression of miR‑130a, which further inhibits the expression of TIMP2 and promotes cervical cancer cell invasion. (spandidos-publications.com)
  • miRNA-149 inhibits the biological behavior of gastric cancer by targeting FOXC1, providing a promising target in the treatment of human gastric cancer. (hindawi.com)
  • Therefore, targeting this epigenetic signature may be an effective strategy to ameliorate the consequences of HD. (nih.gov)
  • Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treat- ment. (lu.se)
  • Intuitive framework for identifying spatially variable genes (SVGs) via edgeR, a popular method for performing differential expression analyses. (bioconductor.org)
  • Curcumin-Zein Nanospheres Improve Liver Targeting and Antifibroti. (ingentaconnect.com)
  • The current study aimed at exploring the ability of zein (ZN) nanospheres to improve the liver targeting and antifibrotic activity of CUR in a mouse model of carbon tetrachloride (CCl 4 )-induced liver fibrosis. (ingentaconnect.com)
  • We hypothesized that AAV transgene expression could be targeted to specific tissues or cell types by selectively overexpressing AAVR. (nature.com)
  • For example, Cre might disrupt an endogenous mouse gene at its insertion site and cause unanticipated effects of its own, especially when the transgene is bred to homozygosity. (jax.org)
  • We aimed to determine the significance of detecting expression of some antigens that might be pivotal to the process, namely CD44 and CD117, in patients with active pemphigus vulgaris. (who.int)
  • A broad antibody response was detected that included immunoglobulin G (IgG) and IgA subtypes with demonstrable Fc-mediated effector function targeting multiple viral antigens. (cdc.gov)
  • We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)
  • We found that we can use gene expression to help understand whether a rare mutation is causing disease or playing a role in disease development," said Geschwind. (uclahealth.org)
  • This research aimed to determine the levels of IL-22, myeloperoxide (MPO), proliferation and wild-type p53 expression after the administration of DPE to murine models of CAC. (biomedcentral.com)
  • While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
  • This non-viral delivery platform offers significant advantages over traditional gene delivery systems as it can deliver multiple types of nucleic acids, allows for repeat dosing and dose optimization, and can be easily and affordably manufactured. (biospace.com)
  • The Company's non-viral Sleeping Beauty gene transfer platform has enabled the development of a unique TCR-T Library. (archiebronsonoutfit.net)
  • This shows that C. tropicalis promotes CRC progression via controlling the expression of tumor cell-intrinsic PD-1. (jcancer.org)
  • These findings demonstrate that direct targeting of GRM1 is a necessary and highly specific driver event for the development of CMF. (medscape.com)
  • Gene expression offers a molecular signpost pointing scientists in the right direction by narrowing the field and highlighting specific areas of the genome. (uclahealth.org)
  • This innovative design enables sensitive and specific detection of target genes in tissue or cell samples while preserving the morphology. (enzo.com)
  • 11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
  • 18 , 19 We surmised that cyclic cMOs containing an NfsB-cleavable linker could be used with analogous lines to knock down gene function in a tissue-specific manner (Figure 1 a). (cdc.gov)
  • Correction: Expression of hormone receptors is associated with specific immunological profiles of the breast cancer microenvironment. (medscape.com)
  • Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)
  • Clinical studies by Stanford University showed that there are 2265 genes in the human body which control the skin-aging process^3. (psmedical.com.hk)